GB2017125A - Polyglutaraldehyde synthesis and protein bonding substrates - Google Patents

Polyglutaraldehyde synthesis and protein bonding substrates

Info

Publication number
GB2017125A
GB2017125A GB7909329A GB7909329A GB2017125A GB 2017125 A GB2017125 A GB 2017125A GB 7909329 A GB7909329 A GB 7909329A GB 7909329 A GB7909329 A GB 7909329A GB 2017125 A GB2017125 A GB 2017125A
Authority
GB
United Kingdom
Prior art keywords
polyglutaraldehyde
pgl
synthesis
microspheres
suspension polymerization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB7909329A
Other versions
GB2017125B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
California Institute of Technology CalTech
Original Assignee
California Institute of Technology CalTech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California Institute of Technology CalTech filed Critical California Institute of Technology CalTech
Publication of GB2017125A publication Critical patent/GB2017125A/en
Application granted granted Critical
Publication of GB2017125B publication Critical patent/GB2017125B/en
Expired legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/44—Antibodies bound to carriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/096—Polyesters; Polyamides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/5094—Microcapsules containing magnetic carrier material, e.g. ferrite for drug targeting
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G6/00—Condensation polymers of aldehydes or ketones only
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/5434—Magnetic particles using magnetic particle immunoreagent carriers which constitute new materials per se
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2446/00—Magnetic particle immunoreagent carriers
    • G01N2446/20—Magnetic particle immunoreagent carriers the magnetic material being present in the particle core
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2446/00—Magnetic particle immunoreagent carriers
    • G01N2446/30—Magnetic particle immunoreagent carriers the magnetic material being dispersed in the polymer composition before their conversion into particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2446/00—Magnetic particle immunoreagent carriers
    • G01N2446/40—Magnetic particle immunoreagent carriers the magnetic material being dispersed in the monomer composition prior to polymerisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2446/00—Magnetic particle immunoreagent carriers
    • G01N2446/60—Magnetic particle immunoreagent carriers the magnetic material being dispersed in a medium other than the main solvent prior to incorporation into the polymer particle

Abstract

Polyglutaraldehyde (PGL) is polymerized in aqueous base or in aqueous highly polar solvent basic media, by raising the pH of the solution to above 7, to prepare powders, castable films or coatings for substrates such as amine substituted microbeads. PGL microspheres can be prepared by suspension polymerization in presence of a surfactant or by precipitating PGL from solution containing surfactant. Magnetic PGL microspheres are formed by suspension polymerization in the presence of magnetic particles such as iron oxide. Polyglutaraldehyde can be converted to a fluorescent polymer by reaction with m-amino- phenol or other reagent. Proteins can be readily covalently bound to the polyglutaraldehyde.
GB7909329A 1978-03-17 1979-03-16 Polyglutaraldehyde synthesis and protein bonding substrates Expired GB2017125B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US88782578A 1978-03-17 1978-03-17

Publications (2)

Publication Number Publication Date
GB2017125A true GB2017125A (en) 1979-10-03
GB2017125B GB2017125B (en) 1982-07-21

Family

ID=25391942

Family Applications (1)

Application Number Title Priority Date Filing Date
GB7909329A Expired GB2017125B (en) 1978-03-17 1979-03-16 Polyglutaraldehyde synthesis and protein bonding substrates

Country Status (3)

Country Link
JP (1) JPS54130696A (en)
DE (1) DE2910414A1 (en)
GB (1) GB2017125B (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0087786A1 (en) * 1982-02-28 1983-09-07 Yeda Research And Development Company, Ltd. Agarose-polyaldehyde beads, process for their production and their use
EP0088022B1 (en) * 1982-02-26 1987-11-11 Institut Pasteur Improved antitumour agents, their preparation and process to enhance the activity of antitumour agents
EP0369515A1 (en) * 1988-11-14 1990-05-23 Akzo Nobel N.V. Aqueous suspension for diagnostic tests
EP0441468A2 (en) * 1990-02-09 1991-08-14 Schering Aktiengesellschaft Gas-containing microparticles composed of polyaldehydes as contrast agents
WO1994004918A1 (en) * 1992-08-26 1994-03-03 James Howard Slater A method of marking a liquid
US6013531A (en) * 1987-10-26 2000-01-11 Dade International Inc. Method to use fluorescent magnetic polymer particles as markers in an immunoassay
WO2006079016A1 (en) * 2005-01-20 2006-07-27 Luminex Corporation Magnetic microspheres for use in fluorescence-based applications
EP2055727A1 (en) * 2007-10-30 2009-05-06 Corning Incorporated High-capacity nanoparticulate immobilization surface
US8747677B2 (en) 2010-04-09 2014-06-10 Luminex Corporation Magnetic separation device

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4279787A (en) * 1979-07-30 1981-07-21 Tetra Consultants Inc. Method of binding antigens to insoluble polymeric substances
JP3662347B2 (en) * 1996-06-10 2005-06-22 日鉄鉱業株式会社 Medical powder

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4035316A (en) * 1975-11-24 1977-07-12 California Institute Of Technology Cell specific, variable density, polymer microspheres
US4070246A (en) * 1976-04-09 1978-01-24 Abbott Laboratories Reactive matrices

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0088022B1 (en) * 1982-02-26 1987-11-11 Institut Pasteur Improved antitumour agents, their preparation and process to enhance the activity of antitumour agents
EP0087786A1 (en) * 1982-02-28 1983-09-07 Yeda Research And Development Company, Ltd. Agarose-polyaldehyde beads, process for their production and their use
US6013531A (en) * 1987-10-26 2000-01-11 Dade International Inc. Method to use fluorescent magnetic polymer particles as markers in an immunoassay
EP0369515A1 (en) * 1988-11-14 1990-05-23 Akzo Nobel N.V. Aqueous suspension for diagnostic tests
EP0441468A2 (en) * 1990-02-09 1991-08-14 Schering Aktiengesellschaft Gas-containing microparticles composed of polyaldehydes as contrast agents
EP0441468A3 (en) * 1990-02-09 1992-05-27 Schering Aktiengesellschaft Berlin Und Bergkamen Contrast agents composed of polyaldehydes
WO1994004918A1 (en) * 1992-08-26 1994-03-03 James Howard Slater A method of marking a liquid
US5643728A (en) * 1992-08-26 1997-07-01 Slater; James Howard Method of marking a liquid
CN101650998A (en) * 2005-01-20 2010-02-17 卢米尼克斯股份有限公司 Microspheres, populations of microspheres, and methods for forming microspheres
WO2006079016A1 (en) * 2005-01-20 2006-07-27 Luminex Corporation Magnetic microspheres for use in fluorescence-based applications
US7718262B2 (en) 2005-01-20 2010-05-18 Luminex Corporation Magnetic microspheres for use in fluorescence-based applications
EP2244270A3 (en) * 2005-01-20 2011-02-23 Luminex Corporation Microspheres having fluorescent and magnetic properties
US8283037B2 (en) 2005-01-20 2012-10-09 Luminex Corporation Magnetic microspheres for use in fluorescence-based applications
CN101650998B (en) * 2005-01-20 2013-02-06 卢米尼克斯股份有限公司 Microspheres, populations of microspheres, and methods for forming microspheres
US8568881B2 (en) 2005-01-20 2013-10-29 Luminex Corporation Magnetic microspheres for use in fluorescence-based applications
US8968874B2 (en) 2005-01-20 2015-03-03 Luminex Corporation Magnetic microspheres for use in fluorescence-based applications
US9376613B2 (en) 2005-01-20 2016-06-28 Luminex Corporation Magnetic microspheres for use in fluorescence-based applications
US9645142B2 (en) 2005-01-20 2017-05-09 Luminex Corporation Magnetic microspheres for use in fluorescence-based applications
EP2055727A1 (en) * 2007-10-30 2009-05-06 Corning Incorporated High-capacity nanoparticulate immobilization surface
US8747677B2 (en) 2010-04-09 2014-06-10 Luminex Corporation Magnetic separation device

Also Published As

Publication number Publication date
JPS54130696A (en) 1979-10-11
DE2910414A1 (en) 1979-09-27
GB2017125B (en) 1982-07-21

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