GB201107868D0 - Method and probes for nucleic acid analysis - Google Patents

Method and probes for nucleic acid analysis

Info

Publication number
GB201107868D0
GB201107868D0 GBGB1107868.0A GB201107868A GB201107868D0 GB 201107868 D0 GB201107868 D0 GB 201107868D0 GB 201107868 A GB201107868 A GB 201107868A GB 201107868 D0 GB201107868 D0 GB 201107868D0
Authority
GB
United Kingdom
Prior art keywords
target
oligonucleotide
nucleic acid
vector
complementary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GBGB1107868.0A
Other versions
GB2492042A (en
GB2492042B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agilent Technologies Inc
Original Assignee
Halo Genomics AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Halo Genomics AB filed Critical Halo Genomics AB
Priority to GB1107868.0A priority Critical patent/GB2492042B/en
Publication of GB201107868D0 publication Critical patent/GB201107868D0/en
Publication of GB2492042A publication Critical patent/GB2492042A/en
Application granted granted Critical
Publication of GB2492042B publication Critical patent/GB2492042B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Abstract

A method for detecting or enriching a target nucleic acid in a nucleic acid sample comprising fragmenting the sample into fragments containing said target nucleic acid, rendering said fragments single stranded, contacting the fragments with a partially double-stranded probe comprising a selector oligonucleotide with two non-adjacent target specific regions and a non-target specific region which comprises at least two binding sites for a vector oligonucleotide, and at least one vector oligonucleotide not complementary to the target sequence and which comprises a nucleotide sequence complementary to the vector-binding site of said selector oligonucleotide, said vector oligonucleotide also comprising a region which is not complementary to the selector oligonucleotide which comprises a nucleotide sequence containing element(s) for detection/enrichment, allowing hybridisation of complementary portions of the probe oligonucleotides and the target to occur, ligating said vector oligonucleotide(s) and target to produce a probe-target fragment hybrid, and detecting or enriching for said hybrid.
GB1107868.0A 2011-05-11 2011-05-11 Selector probes for nucleic acid analysis comprising at each end a non-adjacent target specific region Active GB2492042B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1107868.0A GB2492042B (en) 2011-05-11 2011-05-11 Selector probes for nucleic acid analysis comprising at each end a non-adjacent target specific region

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1107868.0A GB2492042B (en) 2011-05-11 2011-05-11 Selector probes for nucleic acid analysis comprising at each end a non-adjacent target specific region

Publications (3)

Publication Number Publication Date
GB201107868D0 true GB201107868D0 (en) 2011-06-22
GB2492042A GB2492042A (en) 2012-12-26
GB2492042B GB2492042B (en) 2020-04-15

Family

ID=44243946

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1107868.0A Active GB2492042B (en) 2011-05-11 2011-05-11 Selector probes for nucleic acid analysis comprising at each end a non-adjacent target specific region

Country Status (1)

Country Link
GB (1) GB2492042B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2520765A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Multiplex detection of nucleic acids
GB2520763A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Nucleic acid probe and method of detecting genomic fragments
ES2788737T3 (en) 2015-09-18 2020-10-22 Vanadis Diagnostics Set of probes to analyze a DNA sample and method to use the same

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7510829B2 (en) * 2001-11-19 2009-03-31 Affymetrix, Inc. Multiplex PCR
GB2382137A (en) * 2001-11-20 2003-05-21 Mats Gullberg Nucleic acid enrichment
SE0401270D0 (en) * 2004-05-18 2004-05-18 Fredrik Dahl Method for amplifying specific nucleic acids in parallel
US20080293589A1 (en) * 2007-05-24 2008-11-27 Affymetrix, Inc. Multiplex locus specific amplification
US20100291636A1 (en) * 2007-06-11 2010-11-18 Olink Genomics Ab Method for introducing common and/or individual sequence elements in a target nucleic acid molecule
GB0921264D0 (en) * 2009-12-03 2010-01-20 Olink Genomics Ab Method for amplification of target nucleic acid

Also Published As

Publication number Publication date
GB2492042A (en) 2012-12-26
GB2492042B (en) 2020-04-15

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Legal Events

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COOA Change in applicant's name or ownership of the application

Owner name: AGILENT TECHNOLOGIES INC

Free format text: FORMER OWNER: HALO GENOMICS AB