GB201103167D0 - Gene silencing - Google Patents

Gene silencing

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Publication number
GB201103167D0
GB201103167D0 GB201103167A GB201103167A GB201103167D0 GB 201103167 D0 GB201103167 D0 GB 201103167D0 GB 201103167 A GB201103167 A GB 201103167A GB 201103167 A GB201103167 A GB 201103167A GB 201103167 D0 GB201103167 D0 GB 201103167D0
Authority
GB
United Kingdom
Prior art keywords
gene
interest
pol
promoter element
dna molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
GB201103167A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford University Innovation Ltd
Original Assignee
Oxford University Innovation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford University Innovation Ltd filed Critical Oxford University Innovation Ltd
Priority to GB201103167A priority Critical patent/GB201103167D0/en
Publication of GB201103167D0 publication Critical patent/GB201103167D0/en
Priority to PCT/GB2012/050410 priority patent/WO2012114111A1/en
Ceased legal-status Critical Current

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides a method of reducing or preventing the expression of a gene of interest comprising providing a DNA molecule having a sequence which comprises in a 5' to 3' direction (i) a Pol II promoter element in sense orientation, (ii) the gene of interest or a portion thereof comprising 40 nucleotides or more, and (iii) a Pol II promoter element in antisense orientation, and expressing the gene of interest incorporated into the DNA molecule in a mammalian cell. It also provides a method of reducing or preventing the expression of a gene of interest in a mammalian cell comprising integrating a Pol II promoter element in antisense orientation downstream (3') of the gene of interest and inducing expression from the antisense promoter. It also provides an in vitro method of preparing a pool of siRNA molecules specific for a gene of interest comprising providing a DNA molecule having a sequence which comprises in a 5' to 3' direction (i) a Pol II promoter element in sense orientation, (ii) the gene of interest or a portion thereof comprising 100 nucleotides or more, and (iii) a Pol II promoter element in antisense orientation, and transcribing the gene of interest in the presence of Dicer, preferably recombinant Dicer, to produce siRNA molecules.
GB201103167A 2011-02-24 2011-02-24 Gene silencing Ceased GB201103167D0 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB201103167A GB201103167D0 (en) 2011-02-24 2011-02-24 Gene silencing
PCT/GB2012/050410 WO2012114111A1 (en) 2011-02-24 2012-02-23 Gene silencing

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB201103167A GB201103167D0 (en) 2011-02-24 2011-02-24 Gene silencing

Publications (1)

Publication Number Publication Date
GB201103167D0 true GB201103167D0 (en) 2011-04-06

Family

ID=43881589

Family Applications (1)

Application Number Title Priority Date Filing Date
GB201103167A Ceased GB201103167D0 (en) 2011-02-24 2011-02-24 Gene silencing

Country Status (2)

Country Link
GB (1) GB201103167D0 (en)
WO (1) WO2012114111A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2014337506B2 (en) * 2013-10-14 2020-10-15 Ionis Pharmaceuticals, Inc. Methods for modulating expression of C9ORF72 antisense transcript
WO2015057727A1 (en) * 2013-10-14 2015-04-23 Isis Pharmaceuticals, Inc. Compositions for modulating expression of c9orf72 antisense transcript
US11162096B2 (en) 2013-10-14 2021-11-02 Ionis Pharmaceuticals, Inc Methods for modulating expression of C9ORF72 antisense transcript
WO2016112132A1 (en) 2015-01-06 2016-07-14 Ionis Pharmaceuticals, Inc. Compositions for modulating expression of c9orf72 antisense transcript
US10407678B2 (en) 2015-04-16 2019-09-10 Ionis Pharmaceuticals, Inc. Compositions for modulating expression of C9ORF72 antisense transcript

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001077350A2 (en) 2000-04-07 2001-10-18 Large Scale Biology Corporation Compositions and methods for inhibiting gene expression
CA2501065A1 (en) * 2002-09-04 2004-03-18 Johnson & Johnson Research Pty Ltd Methods using dsdna to mediate rna interference (rnai)
US20050060771A1 (en) * 2003-09-11 2005-03-17 Farmer Andrew Alan siRNA encoding constructs and methods for using the same
US7713735B2 (en) * 2004-09-24 2010-05-11 J.R. Simplot Company Gene silencing
EP2208785A1 (en) * 2009-01-15 2010-07-21 Newbiotechnic, S.A. Methods and kits to generate miRNA- and smallRNA-expressing vectors, and its application to develop lentiviral expression libraries

Also Published As

Publication number Publication date
WO2012114111A1 (en) 2012-08-30

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Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)