GB1533410A - Antigen detecting reagents - Google Patents

Antigen detecting reagents

Info

Publication number
GB1533410A
GB1533410A GB5127175A GB5127175A GB1533410A GB 1533410 A GB1533410 A GB 1533410A GB 5127175 A GB5127175 A GB 5127175A GB 5127175 A GB5127175 A GB 5127175A GB 1533410 A GB1533410 A GB 1533410A
Authority
GB
United Kingdom
Prior art keywords
reactant
molecules
polymer
coupled
dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB5127175A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BLOCK ENG Inc
BLOCK ENGINEERING
Original Assignee
BLOCK ENG Inc
BLOCK ENGINEERING
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US05/535,095 priority Critical patent/US4166105A/en
Priority to US05/535,111 priority patent/US4169137A/en
Application filed by BLOCK ENG Inc, BLOCK ENGINEERING filed Critical BLOCK ENG Inc
Publication of GB1533410A publication Critical patent/GB1533410A/en
Application status is Expired legal-status Critical

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • G01N33/533Production of labelled immunochemicals with fluorescent label

Abstract

1533410 Radiant energy emitting polymeric analytical reagent BLOCK ENG Inc 15 Dec 1975 [26 Dec 1974 (2)] 51271/75 Addition to 1470074 Heading C3R [Also in Division G1] The invention comprises polymer molecules covalently bonded to first reactant molecules which are capable of coupling with second reactant molecules, each polymer molecule also having attached to it a plurality of fluorescent dye molecules and the molecular ratio between the polymer molecules and the first reactant molecules being substantially 1 : 1. The presence of second reactant (or analyte) may be detected by coupling to it the first reactant/ polymer/dye complex and observing fluorescence. The polymer may be a polyethylenimine and dye molecules, e.g. fluorescein, may be attached to nitrogen atoms along the polymer chains, reactive end-groups of the polymer being temporarily blocked, e.g. with an aldehyde, while the dye molecules are attached; the polymer with dye molecules attached is then coupled to the first reactant. The first reactant may be an antibody and the second reactant (analyte) may be an antigen; also, the first reactant may be a ligand and the second reactant may be a polyvalent metal. In examples polyethylenimines are coupled with fluorescein and polylysine is coupled with lissamine Rhodamine-B; polyamine/dye complexes are then coupled with Anti-Echo virus antiserum (antibody) and used to detect Hepatitis B antigen; also, a polyamine/dye complex is coupled with benzyl p-amino diisonitrosoethane (a ligand) and used to detect nickel.
GB5127175A 1973-07-30 1975-12-15 Antigen detecting reagents Expired GB1533410A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US05/535,095 US4166105A (en) 1973-07-30 1974-12-20 Dye tagged reagent
US05/535,111 US4169137A (en) 1974-12-20 1974-12-20 Antigen detecting reagents

Publications (1)

Publication Number Publication Date
GB1533410A true GB1533410A (en) 1978-11-22

Family

ID=27064710

Family Applications (1)

Application Number Title Priority Date Filing Date
GB5127175A Expired GB1533410A (en) 1973-07-30 1975-12-15 Antigen detecting reagents

Country Status (6)

Country Link
JP (1) JPS51106721A (en)
AU (1) AU8770775A (en)
DE (1) DE2557419C2 (en)
FR (1) FR2295426A2 (en)
GB (1) GB1533410A (en)
NL (1) NL7514864A (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1111762A (en) * 1977-12-28 1981-11-03 David S. Frank Fluorescent rare earth chelate in polymeric latex particles
CA1121345A (en) * 1979-03-05 1982-04-06 Robert A. Yoshida Method for competitive protein binding assays inhibiting non-specific interference
NL8000173A (en) 1980-01-11 1981-08-03 Akzo Nv Use of water-dispersible, hydrophobic dyes as labels in immunochemical assays.
IT1140209B (en) * 1981-09-25 1986-09-24 Anic Spa Reagents for immunoluorescenza and method for their preparation
US4434150A (en) * 1981-10-19 1984-02-28 Ortho Diagnostic Systems, Inc. Immunological reagents employing polymeric backbone possessing reactive functional groups
AT377095B (en) * 1982-11-23 1985-02-11 List Hans Sensor element for the determination of a sample O2 content and methods for producing same
US4687732A (en) * 1983-06-10 1987-08-18 Yale University Visualization polymers and their application to diagnostic medicine
EP0134307B1 (en) * 1983-07-29 1989-04-19 Henning Berlin GmbH Chemie und Pharmawerk Preactivated surfaces of synthetic materials for immobilizing organochemical and biological substances, process for preparing them and their use
US4959306A (en) * 1986-11-28 1990-09-25 Sclavo, Inc. Labeling design for a binding assay reagent
DE3921498A1 (en) * 1988-09-28 1990-03-29 Bayer Ag Polymer-bound dyes, processes for their preparation and use
US5019521A (en) * 1990-03-12 1991-05-28 Photest Diagnostics, Inc. Paired polypeptides
DE4114482A1 (en) * 1991-05-03 1992-11-05 Bayer Ag Polymeric dyes, processes for their preparation and their use
US5750357A (en) * 1994-05-18 1998-05-12 Microquest Diagnostics, Inc. Method of rapid analyte detection
JP4848988B2 (en) * 2007-03-26 2011-12-28 セイコーエプソン株式会社 Detection kit

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3880934A (en) * 1972-02-10 1975-04-29 Syntex Inc Nitrophenyloxy-butanediols

Also Published As

Publication number Publication date
AU8770775A (en) 1977-06-23
DE2557419C2 (en) 1986-09-04
FR2295426A2 (en) 1976-07-16
NL7514864A (en) 1976-06-22
DE2557419A1 (en) 1976-06-24
JPS51106721A (en) 1976-09-21

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee