GB1385320A - Cholesterol assay - Google Patents
Cholesterol assayInfo
- Publication number
- GB1385320A GB1385320A GB3306874A GB3306874A GB1385320A GB 1385320 A GB1385320 A GB 1385320A GB 3306874 A GB3306874 A GB 3306874A GB 3306874 A GB3306874 A GB 3306874A GB 1385320 A GB1385320 A GB 1385320A
- Authority
- GB
- United Kingdom
- Prior art keywords
- amount
- cholesterol
- hydrogen peroxide
- cholestenone
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
Abstract
1385320 Determination of cholesterol NATIONAL RESEARCH DEVELOPMENT CORP 18 Sept 1972 [22 Sept 1971 19 June 1972] 33069/74 and 33068/74 Divided out of 1385319 Heading G1B A method of assaying for cholesterol in a liquid (e.g. serum) comprises incubating the liquid with an enzyme preparation capable of oxidizing the cholesterol into #4-cholestenone and hydrogen peroxide and determining the amount of cholesterol present by measuring the amount of hydrogen peroxide or #4-cholestenone produced or the amount of oxygen absorbed in the reaction. Preferably the enzyme is obtained from a microorganism of the group Mycobacterium rhodocrous e.g. Nocardia species NCIB 10554 or NCIB 10555 wherein the enzyme preparation has a cholesterol oxidase specific activity of at least 1 unit per 5mg of protein nitrogen and a potency of at least 10<SP>-2</SP> units/ml. The amount of hydrogen peroxide may be measured fluorimetrically or by means of a chromogenic reagent, for example by means of quadrivalent titanium and xylenol oranges or by reaction of the hydrogen peroxide with 4-aminophenazone in the presence of phenol and peroxidase, the product being measured colorimetrically. Other reagents are mentioned. The amount of oxygen taken up may be measured by means of an oxygen electrode and the amount of #<SP>4</SP>-cholestenone may be determined by its carbonyl absorption or a derivative of #4-cholestenone such as its 2, 4 diphenyl hydrazone or iso nicotinic acid hydrazone is formed and is measured colorimetrically. A kit for use in assaying the amount in a liquid comprises in association: (i) an enzyme preparation capable of oxidizing cholesterol into #4-cholestenone and hydrogen peroxide preferably as defined above and (ii) at least one reagent which is capable of being used in the determination of the amount in which hydrogen peroxide or #<SP>4</SP>-cholestenone is formed. The reagent (ii) may be a reagent which takes part in a reaction by means of which hydrogen peroxide may be determined fluorimetrically or colorimetrically and the kit may comprise additionally at least one reagent capable of being used in hydrolyzing serum for serum cholesterol assays.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB3306874A GB1385320A (en) | 1971-09-22 | 1972-06-19 | Cholesterol assay |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB3306974 | 1971-09-22 | ||
GB3306874A GB1385320A (en) | 1971-09-22 | 1972-06-19 | Cholesterol assay |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1385320A true GB1385320A (en) | 1975-02-26 |
Family
ID=26261702
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB3306874A Expired GB1385320A (en) | 1971-09-22 | 1972-06-19 | Cholesterol assay |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB1385320A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0016947A1 (en) * | 1979-04-04 | 1980-10-15 | Roche Diagnostics GmbH | Process and reagent for the enzymatic determination of enzymatic substrates |
US4247631A (en) * | 1979-01-31 | 1981-01-27 | Millipore Corporation | Reagent and method for the analytic determination of hydrogen peroxide |
US6737275B2 (en) | 2001-02-05 | 2004-05-18 | The Board Of Regents For Oklahoma State University | Direct serum lipids assays for evaluation of disease states |
-
1972
- 1972-06-19 GB GB3306874A patent/GB1385320A/en not_active Expired
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4247631A (en) * | 1979-01-31 | 1981-01-27 | Millipore Corporation | Reagent and method for the analytic determination of hydrogen peroxide |
FR2476129A1 (en) * | 1979-01-31 | 1981-08-21 | Millipore Corp | METHOD FOR PRODUCING DYE FOR DETECTING THE PRESENCE OF HYDROGEN PEROXIDE IN AQUEOUS MEDIUM, METHOD FOR ENZYMATIC DETERMINATION OF THE QUANTITY OF A COMPONENT IN A BODILY FLUID, AND REACTIVE SYSTEM FOR USE |
EP0016947A1 (en) * | 1979-04-04 | 1980-10-15 | Roche Diagnostics GmbH | Process and reagent for the enzymatic determination of enzymatic substrates |
US6737275B2 (en) | 2001-02-05 | 2004-05-18 | The Board Of Regents For Oklahoma State University | Direct serum lipids assays for evaluation of disease states |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed | ||
732 | Registration of transactions, instruments or events in the register (sect. 32/1977) | ||
PE20 | Patent expired after termination of 20 years |