GB1351836A - Method of measuring serum thyroxine and apparatus for use therein - Google Patents

Method of measuring serum thyroxine and apparatus for use therein

Info

Publication number
GB1351836A
GB1351836A GB3027671A GB3027671A GB1351836A GB 1351836 A GB1351836 A GB 1351836A GB 3027671 A GB3027671 A GB 3027671A GB 3027671 A GB3027671 A GB 3027671A GB 1351836 A GB1351836 A GB 1351836A
Authority
GB
United Kingdom
Prior art keywords
thyroxine
column
serum
determination
gel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3027671A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yissum Research Development Co of Hebrew University of Jerusalem
Original Assignee
Yissum Research Development Co of Hebrew University of Jerusalem
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Research Development Co of Hebrew University of Jerusalem filed Critical Yissum Research Development Co of Hebrew University of Jerusalem
Publication of GB1351836A publication Critical patent/GB1351836A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/804—Radioisotope, e.g. radioimmunoassay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/825—Pretreatment for removal of interfering factors from sample
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/826—Additives, e.g. buffers, diluents, preservatives

Abstract

1351836 Determination of thyroxine YISSUM RESEARCH DEVELOPMENT CO OF THE HEBREW UNIVERSITY OF JERUSALEM 28 June 1971 [29 June 1970] Heading G1B Apparatus for determination of thyroxine in serum comprises a tubular body having closure means at both ends and containing a column of dextran gel cross-linked with epichlorhydrin and having a water regain of 1 to 5 gm per gm dry gel, the dextran gel being in equilibrium with an aqueous medium having a pH of at least 11. The apparatus may comprise a syringe barrel closed by polyethylene caps, the gel being retained between porous sintered polyethylene discs. In carrying out the determination, predetermined amounts of the serum and of radioactive thyroxine solution are allowed to flow into the column, the column is washed with an aqueous alkaline solution pH 8 to 10 to remove protein, a predetermined quantity of a binding agent for the thyroxine, for example human or bovine serum, human alpha globulin or bovine gamma globulin is added.to the column to partially remove the thyroxine from the column, the ratio of radioactive thyroxine retained by the column to that originally added is determined, and the thyroxine content of the serum is calculated by comparing the ratio determined and ratios obtained with known concentrations of thyroxine in serum.
GB3027671A 1970-06-29 1971-06-28 Method of measuring serum thyroxine and apparatus for use therein Expired GB1351836A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US5100570A 1970-06-29 1970-06-29

Publications (1)

Publication Number Publication Date
GB1351836A true GB1351836A (en) 1974-05-01

Family

ID=21968790

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3027671A Expired GB1351836A (en) 1970-06-29 1971-06-28 Method of measuring serum thyroxine and apparatus for use therein

Country Status (11)

Country Link
US (1) US3659104A (en)
JP (1) JPS5331399B1 (en)
CA (1) CA955159A (en)
DE (1) DE2132112C3 (en)
ES (1) ES392664A1 (en)
FR (1) FR2100008A5 (en)
GB (1) GB1351836A (en)
HU (1) HU166424B (en)
IL (1) IL37046A (en)
SE (1) SE392346B (en)
ZA (1) ZA713408B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0021214A1 (en) * 1979-06-21 1981-01-07 Ames-Yissum Limited Multilayer column chromatography specific binding assay method, test device and test kit

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3929410A (en) * 1970-11-09 1975-12-30 Benjamin Schloss Analytical process
DE2129553A1 (en) * 1971-06-15 1972-12-21 Merck Patent Gmbh Method for the determination of thyroxine
NL7112927A (en) * 1971-09-21 1973-03-23
US3753655A (en) * 1971-11-09 1973-08-21 B Schreiber Process for isolation and separation of thyroid hormones
US3911096A (en) * 1972-06-23 1975-10-07 Professional Staff Ass Of The Radioimmunoassay for measurement of thyroxine (T{HD 4{B ) and triiodothyonine (T{HD 3{B ) in blood serum
USRE32098E (en) * 1972-06-23 1986-03-25 Research And Education Institute, Inc. Radioimmunoassay for measurement of thyroxine (T4) and triiodothyronine (T3) in blood serum
IL42105A (en) * 1973-04-25 1976-08-31 Yissum Res Dev Co Process for determination of triiodothyronine
GB1412274A (en) * 1973-05-29 1975-11-05 Bio Rad Laboratories Radioactive determination of serum thyroxine
US3941564A (en) * 1973-09-13 1976-03-02 Miles Laboratories, Inc. Method for assessing thyroid function
US4230797A (en) * 1975-04-28 1980-10-28 Miles Laboratories, Inc. Heterogenous specific binding assay employing a coenzyme as label
IT1206354B (en) * 1977-03-10 1989-04-21 Lepetit Spa KIT TO BE USED FOR THE DETERMINATION OF FREE HORMONES IN BIOLOGICAL FLUIDS
US4170454A (en) * 1978-03-30 1979-10-09 Union Carbide Corporation Process for the preparation of a solid-phase radioimmunoassay support and use thereof
US4318980A (en) * 1978-04-10 1982-03-09 Miles Laboratories, Inc. Heterogenous specific binding assay employing a cycling reactant as label
US4492751A (en) * 1978-04-10 1985-01-08 Miles Laboratories, Inc. Heterogenous specific binding assay employing an enzyme substrate as label
US4225576A (en) * 1978-11-20 1980-09-30 Miles Laboratories, Inc. Combined radioimmunoassay for triiodothyronine and thyroxine
US5217903A (en) * 1990-05-15 1993-06-08 Trustees Of Boston University Measuring connective tissue breakdown products in body fluids
KR20020065698A (en) * 2001-02-07 2002-08-14 주식회사 바이오라인 Detection kit of thyroid hormone using ria

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3507618A (en) * 1964-11-27 1970-04-21 Squibb & Sons Inc Apparatus and method for determining thyroid function
US3451777A (en) * 1965-08-20 1969-06-24 Walter Di Giulio Method and apparatus for determining the thyroid hormone content of blood

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0021214A1 (en) * 1979-06-21 1981-01-07 Ames-Yissum Limited Multilayer column chromatography specific binding assay method, test device and test kit

Also Published As

Publication number Publication date
IL37046A (en) 1974-05-16
DE2132112A1 (en) 1972-01-27
JPS5331399B1 (en) 1978-09-02
ES392664A1 (en) 1973-08-01
SE392346B (en) 1977-03-21
CA955159A (en) 1974-09-24
DE2132112C3 (en) 1974-09-19
US3659104A (en) 1972-04-25
HU166424B (en) 1975-03-28
ZA713408B (en) 1972-01-26
FR2100008A5 (en) 1972-03-17
DE2132112B2 (en) 1974-02-21

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee