GB1323521A - Diffusion method for determination of enzymes - Google Patents
Diffusion method for determination of enzymesInfo
- Publication number
- GB1323521A GB1323521A GB1323521DA GB1323521A GB 1323521 A GB1323521 A GB 1323521A GB 1323521D A GB1323521D A GB 1323521DA GB 1323521 A GB1323521 A GB 1323521A
- Authority
- GB
- United Kingdom
- Prior art keywords
- plate
- determination
- dpn
- enzyme
- tpn
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
1323521 Gel plate for enzyme determination BAXTER LABORATORIES INC 22 Jan 1971 3024/71 Heading B1X An agar gel plate for use in a micro method for the quantitative determination of low levels of DPN-or TPN-linked enzyme activity comprises a shallow plate carrying an agar gel medium buffered to a pH of 6 to 9 and containing 0.2 to 4% agar, DPN or TPN, a substrate for the enzyme and a chromogenic or fluorogenic substance. The plate may be packed in metal foil or plastics film. For the determination, the sample under test is diffused from a 1 to 10 mm within the gel medium and the diameter of the coloured or fluorescent radial diffusion zone produced is measured and compared with that obtained with control samples. The DPN or TPN &c may be incorporated into the gel medium when the plate is prepared or alternatively a solution of these components is laid on the plate after the plate has been incubated with the test sample in the wells, and the plate incubated again. Examples relate to determination of LDH using DPN, lithium lactate, p-nitrotetrazolium blue, phenazine methosulphate and cyanide ion; other enzyme-substrate systems are mentioned.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB302471 | 1971-01-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1323521A true GB1323521A (en) | 1973-07-18 |
Family
ID=9750532
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1323521D Expired GB1323521A (en) | 1971-01-22 | 1971-01-22 | Diffusion method for determination of enzymes |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB1323521A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2147415A (en) * | 1983-08-05 | 1985-05-09 | Powell & Scholefield Limited | Device and method for antibiotic sensitivity testing |
| EP0924302A2 (en) * | 1997-12-19 | 1999-06-23 | Unitika Ltd. | Test strip for creatine kinase activity measurement |
-
1971
- 1971-01-22 GB GB1323521D patent/GB1323521A/en not_active Expired
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2147415A (en) * | 1983-08-05 | 1985-05-09 | Powell & Scholefield Limited | Device and method for antibiotic sensitivity testing |
| EP0924302A2 (en) * | 1997-12-19 | 1999-06-23 | Unitika Ltd. | Test strip for creatine kinase activity measurement |
| EP0924302A3 (en) * | 1997-12-19 | 2000-02-23 | Unitika Ltd. | Test strip for creatine kinase activity measurement |
| US6130054A (en) * | 1997-12-19 | 2000-10-10 | Unitika Ltd. | Test strip for creatine kinase activity measurement |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PCNP | Patent ceased through non-payment of renewal fee |