FR2849601A1 - Synergistic, plant extract-based, non-solid oral composition, useful e.g. for reducing weight or stress, contains vitamins and minerals or trace elements to improve bioavailability and efficacy - Google Patents

Abstract

The use of a synergistic, orally administered, non-solid composition (I) is claimed for improving the efficacy of plant extracts. (I) Comprises (A) plant extracts, (B) at least two of vitamins A, B2, B9 and E and (C) at least two minerals or trace elements selected from magnesium, zinc, copper and selenium, for stimulating cellular metabolism. An Independent claim is included for a combination of (I) with an excipient acceptable in foodstuffs.

Description

The present invention relates to the use of

  synergistic compositions based on plant extracts with drinks rich in vital elements which improve their effectiveness.

  It is well known that plants contain different natural compounds which influence certain physiological functions of the organism. Many natural compounds have thus been isolated from plants such as white willow aspirin (Salix alba), caffeine from coffee 10 (Coffea spp), artichoke cynarin (Cynara scolymus), from fungi such as penicillium penicillin or algae. Plants containing an active compound, for example aspirin from Salix alba, are not directly used as a medicament since the active molecules which they contain must be identified, isolated and chemically synthesized to be effective. The first cause which prevents the direct use of plants or plant extracts is that it may be necessary to consume the active parts of plants in very large quantities to obtain a satisfactory effect, which makes their use difficult. .

  The second cause that limits the effectiveness of herbal products is their poor oral absorption. Very often, powders or dried plants, plant extracts, can be used orally but the active fraction remains very low or is very little released in the stomach or intestine to be absorbed in the digestive tract.

  The third cause which limits the use of plants is linked to their very bad taste which prevents their direct use by the oral route, which requires carrying out specific technological operations (de-amerization, deodorization) or using capsules 35 which reduce the efficiency of plants.

  Finally, the fourth cause of lack of activity or higher toxicity of medicinal plants is the weakness of the metabolic activity of the cells of the body. For optimal cellular metabolism, cells need sufficient amounts of certain specific vital elements such as vitamins, minerals and trace elements which act as catalysts for metabolism. Scientific and epidemiological studies show that around 7 in 10 people have a more or less complete deficiency with respect to at least one vitamin, mineral or trace element because they do not receive the Recommended Daily Allowance (RDA) from 10 these elements, daily contributions which are nevertheless essential (SOUCCAR T. CURTAY JP The new vitamin guide, Editions du Seuil, Paris, 1996.). As these vital elements act in synergy and regulate most of the functions of the cells of our organism, the lack of only one of them can disrupt the whole cellular or organism metabolism and generate numerous physical or psychological disorders such as overweight, intestinal transit problems, accelerated hair loss, stress, anxiety, fatigue, depressive state, headache, memory loss, sleep disturbances, lack of vitality, tone, lack of sexual desire, joint pain, disorders which may be aggravated by the hormonal status of individuals such as hot flashes, osteoporosis or increased cholesterol level.

  Several plants are known for their beneficial effects but their general use remains limited because of the four drawbacks mentioned above, the need to consume a large quantity of the plants or their extracts; poor oral absorption in the stomach or intestine; inevitable technological transformations altering the efficiency of plants; decrease in cellular metabolism due to the lack of certain vital elements. The effectiveness of therapeutic plants is therefore generally limited and much less important than the molecules obtained by chemical synthesis.

  In the course of our research, we discovered that certain very specific combinations of vitamins, minerals and trace elements stimulate cell metabolism in vitro and improve the efficiency of plant extracts (see pharmacological study). The present invention therefore relates to the use of a non-solid synergistic composition 5 orally to stimulate the efficacy of plant extracts, characterized in that it comprises plant extracts and at least two vitamins chosen from vitamin A, vitamin B2, vitamin B9, vitamin E with at least two minerals or trace elements chosen from magnesium, zinc, copper and selenium to stimulate cell metabolism. The Applicant has also surprisingly discovered that drinks such as fruit juices are naturally rich in these elements which stimulate cell metabolism such as vitamin A, vitamin B6, vitamin B9, vitamin E, certain minerals such as magnesium. and certain trace elements such as zinc, copper, selenium. (PERCIVAL S. et al. Passion fruit juice: a review of the literature. Food Science and Human nutrition, 20 September 2000, pp. 1-17). For example, certain drinks such as passion fruit juice (Passiflora edulis) naturally have proportions of vitamins, minerals and trace elements ideal for stimulating cell metabolism which improves the efficiency of plant extracts. Efficacy tests have shown that the activity of many plant extracts is greatly improved when combined with certain drinks such as fruit juices or vegetable juices which contain contents very close to combinations of vitamins, 30 minerals and trace elements that stimulate cell metabolism. The combination of these drinks with plant extracts has made it possible to obtain a strong improvement in the activity of plant extracts and a marked reduction in their side effects.

  These compositions also make it possible to accelerate the speed of activity of plant extracts, the effect of which becomes greater and faster. These synergistic compositions of plant extracts and drinks have a surprisingly pleasant taste without altering the effectiveness of plant extracts.

  By "drinks" is meant both fruit juices and vegetable juices (tomato, carrot), nectars, still waters or mineral waters (naturally carbonated or carbonated), sodas, products based on milk, fermented or unfermented drinks such as beer, cider, wine, and generally any other liquid form enriched with vitamins, minerals and trace elements to stimulate cell metabolism and physiological functions of the body. The present invention therefore relates to the use of oral synergistic compositions combining plant extracts and liquids naturally rich or enriched with vitamins, minerals and trace elements which stimulate cell metabolism in order to improve bioavailability and efficiency. plants or active compounds present in plant extracts.

  Indeed, the Applicant has discovered that the use of compositions combining drinks rich in vital elements such as vitamins, minerals or trace elements surprisingly improves the absorption, the bioavailability and the effectiveness of almost all of the compounds. active ingredients in plant extracts. This leads to an increase in therapeutic effects and / or a decrease in possible toxic effects. It is essential to use appropriate proportions of vitamins, minerals and trace elements, or sources of these vital elements in particular proportions, to enhance the effects of plant extracts.

  Furthermore, these drinks which improve the efficiency of plant extracts have an excellent taste which facilitates their use by the oral route and thus authorizes the possible consumption of large quantities. When the liquid forms containing these elements are used orally with plant extracts, the pharmacological activity is greatly improved, probably due to an optimization of cell metabolism in general.

  Synergistic compositions as defined above have never been described.

  This is why the present application relates to the use of synergistic compositions containing plant extracts associated with drinks rich in vitamins, minerals and trace elements which make it possible to stimulate cell metabolism.

  The fruit juices rich in vitamins, minerals and trace elements used in the constitution of these compositions can come from different origins for 15 beings associated with plant extracts in different forms, the methods of preparation of which are familiar to those skilled in the art. 'art.

  In the present application and in what follows, the term "composition" according to the invention refers to non-solid preparations (liquids, semi-liquids, viscous).

  Under preferential conditions for implementing the invention, the compositions are food, dietetic or pharmaceutical preparations for use by the oral route.

  Under preferential conditions for implementing the invention, such a non-solid composition can thus be in the form of a directly drinkable solution based on fresh fruit juice, reconstituted juice or concentrate to be diluted, concentrated syrup or single dose ampoules containing 30 plant extracts.

  According to an advantageous embodiment, the composition making it possible to improve the activity of plant extracts results from the use of agents allowing the supply of the organism with vitamins, minerals and trace elements.

  Under preferential conditions of implementation, the agents allowing the improvement of the activity of plant extracts may be chosen from the usual synthetic or natural forms of vitamin supply, a source or an extract rich in vitamins.

  Under preferential conditions of implementation, the agents allowing the improvement of the activity of the plant extracts 5 may be chosen from the usual synthetic or natural intake forms of minerals and trace elements, a source or an extract rich in minerals or trace elements.

  Such agents are for example marketed by the company QUIMDIS (France) or by the company COOPER (French Pharmaceutical Cooperation, France).

  The usual dose, which varies according to the composition chosen, can for example be administered daily for a period at least equal to 14 days, preferably 30 days, at doses of between 15 and 200 percent of each person's Recommended Daily Allowance (RDA). of these elements, more particularly 50% of the RDI. By RDA is meant the amount of micronutrients actually available for use by the body and not the gross weight of the form of intake used.

  Under preferential conditions of implementation, these compositions are used to accelerate the drainage of the organism, to reduce weight, to act on obesity, to improve intestinal transit, to decrease waist size, to combat hair loss. , decrease stress, anxiety, fatigue, improve depressive states, decrease the frequency or intensity of headaches, improve memory, sleep, tone, fitness, sexual desire, decrease pain joint disorders, age-related disorders such as hot flashes, osteoporosis or cholesterol levels.

  Depending on the problem, these compositions can be combined with a drug, a food or a low-calorie diet, a nutritional or cosmetic product to obtain synergistic effects.

  Under preferential conditions for implementing the invention, various active principles are used, in particular plant extracts.

  By "plant extract" is meant both plants and parts of plants, for example and preferably whole plant juices, liquid, dehydrated, lyophilized or instant plant extracts, essential oils or also extracts of such plants or parts of plants obtained using at least one aqueous and / or organic solvent and which are in a form conventionally used in food, dietetics or pharmacy, liquid or solid.

  Under preferential conditions of implementation, the plant extracts may be chosen from the group of plant extracts naturally containing active principles stimulating thermogenesis, or containing cholagogic and choleretic substances, agents capable of retaining fats in the intestine. , stimulate intestinal transit, the immune system, reduce fatigue, improve strength and vitality.

  Thus, one can find in the compositions according to the invention, one or more substances capable of stimulating thermogenesis, and in particular, caffeine or a plant extract rich in caffeine obtained for example from the seeds of Guarana (Paullinia cupana ), an extract of Green Tea (Thea sinensis), an extract of Cola nuts (Cola nitida); choleretic substances and cholagogues, preferably cynarin or an extract of Artichoke (Cynara scolymus), Rosemary (Rosmarinus officinalis); extracts of diuretic plants, preferably Fennel (Foeniculum vulgare), Dandelion (Taraxacum officinale), Cranberry (Vaccinum oxycoccus); extracts of plants stimulating intestinal transit, preferably tamarind (Tamarindus indica), Senna (Cassia angustifolia); plant extracts capable of stimulating the body's immune system, preferably Ginseng (Panax ginseng), royal jelly, Acerola (Malpighia puncifolia); certain spice plants, preferably Ginger (Zingiber officinalis); essential oils (Citrus limonum, Myristica fragrans, Juniperus communis, Elettaria cardamomum, Cinnamomum zeylanicum); disinfiltrating substances such as enzymes, preferably bromelain or an extract of Pineapple (Ananas comosus) rich in bromelane, papain or an extract of Papaya (Carica papaya) rich in papane; vegetable fibers, in particular fruit fibers (lemon, grapefruit, apple), cereals, pectins, fructo-oligosaccharides, preferably inulin; chitosan; clay, preferably a green clay of the illite type with a phyllitic structure, a montmorrillonite clay, kaolinite, attapulgite, sepiolite, smectite and bentonite.

  Such active ingredients or plant extracts are for example marketed by the company BIOSPHERE (France) or by the company GREENTECH (France) or also or by the company COOPER (French Pharmaceutical Cooperation, France).

  Under preferential processing conditions, the plant extracts may be combined synergistically with the drinks chosen from fruit juices, vegetable juices or soups, nectars, still waters or mineral waters (naturally carbonated or carbonated), sodas, milk-based products, fermented or unfermented drinks such as beer, cider, wine, tea, coffee, whether or not enriched with vital elements as required.

  Thus, one can find in the compositions according to the invention, one or more fruit juices or nectars alone or mixed together and in particular, orange, apple, pineapple, banana, grapefruit, lemon, passion fruit, vegetable juices, preferably tomatoes, carrots, flavored or unflavored tea, coffee, chocolate, sodas made from Cola nuts, Guarana, milk-based drinks, preferably drinking yogurts, liquids based on lactic ferments. These combinations can therefore be in the form of an oral liquid or in the form of a concentrate to be diluted in a liquid. A further subject of the present invention is the use of the compositions described above in combination with a particular diet, an apparatus for physical exercise or for muscular electrostimulation.

  Under preferential conditions of implementation, the particular diets may be chosen from the group of low-calorie diets, high-protein diets, diets improving intestinal transit.

  Under preferential conditions of implementation, the physical exercise or electrostimulation devices 5 may be devices or devices making it possible to stimulate the metabolism.

  The following examples illustrate the present application. These examples of compositions for improving the activity of plant extracts were developed on the basis of the results obtained during pharmacological studies. Certain compositions were then subjected to clinical experiments.

  EXAMPLE 1 A slimming composition was prepared based on pineapple juice corresponding to the following weight formula: Pineapple juice Guarana extract Fennel extract Dandelion extract Fructooligosaccharides Rosemary extract Lemon essential oil Nutmeg essential oil Dosage: About 200 ml all weights 97.5% 0.75% 0.5% 0.375% 0.499125% 0.375% 0.00075% 0.000125% mornings to reduce

EXAMPLE 2:

  A composition has been prepared for improving intestinal transit and waist circumference based on apple juice and 35 grape juices corresponding to the following weight formula: Apple juice White grape juice 73.13 12.19 o Red grape juice 12.19% Lemon fiber 0.5% Oat fiber 0.5% Fructo-oligosaccharides 0.4985% Tamarind extract 0.375% Green tea extract 0.25% Cranberry extract 0.25% Pineapple dry extract 0.125% Juniper essential oil 0.00075% Cardamom essential oil 0 00075% Dosage: About 200 ml every morning and 100 ml in the evening to obtain an improvement in intestinal transit and a decrease in waist circumference 15

EXAMPLE 3

  An invigorating composition has been prepared to improve the form based on orange juice corresponding to the following weight formula: Orange juice 58.721% Banana nectar 39.00% Guarana extract 0.75% Ginseng extract 0.625% Ginger extract 0.5 % Acerola extract 0.375% Magnesium citrate 0.05% Cinnamon essential oil 0.00075% Dosage: A glass every morning and whenever fatigue is manifested.

2849601 there

EXAMPLE 4:

  We have prepared 25 cl bottles of slimming composition based on different drinks corresponding to the following formula: Chicory Inulin Fructose Artichoke Extract (1% cynarin) Maltodextrin Green Tea Extract (3% caffeine) 10 Guarana Extract ( 10% caffeine) Drink 1.22 0.14 0.014 0.0112 0.084 0.084 qs 25 gggggg cl. The beverages used to prepare these vials were fruit juices, vegetable juices, mineral waters, tea, milk, common lager.

  Dosage: use 1 bottle of 25 cl per day.

  EXAMPLE 5 A composition was prepared to improve the intestinal transit and the waist circumference corresponding to the following formula Green clay Magnesium oxide Yeasts enriched with selenium Tamarind Ginger Karaya gum 30 Drink 804 mg 300 mg 72 mg 28 mg 28 mg 24 mg qs 25 cl.

  The beverages used to prepare these vials were fruit juices, vegetable juices, mineral waters, tea, milk and lager beer.

  Dosage: use 1 bottle per day.

  EXAMPLE 6 A slimming composition was prepared corresponding to the following formula: Chicory inulin 1.1558 g Fructose 0.1379 g Soybean 0.0350 g Wheat germ 0.0235 g Artichoke extract (1% cynarin) 0.0133 g Maltodextrin 0.0106 g Guarana extract (10% caffeine) 0.0080 g Green tea extract (3% caffeine) 0.0080 g Oats 0.0035 g Apple fibers 0.0014 g Lithothamne 0.0013 g Yeasts enriched with selenium 0.0004 g Beverage qs 25 cl.

  The beverages used to prepare these vials were fruit juices, vegetable juices, mineral waters, tea, milk and lager for everyday consumption.

  Dosage: use 1 bottle per day.

  EXAMPLE 7 Preparations of approximately 1 liter of a slimming composition were prepared corresponding to the following formula: Vitamin A 0.008 g Vitamin C 0.12 g Vitamin E 0.01 g Iron 0.014 g Zinc 0.015 g Selenium 0.000075 g Pineapple extract 0.3 g Aqueous extract of Green Tea 0.4 g Fennel extract 0.4 g UHT cow's milk qs 1000 g Dosage: use a glass or about 20 cl per day.

  EXAMPLE 8 25 cl bottles of a composition were prepared to improve the strength and vitality corresponding to the following formula Light beer 90 g Guarana fluid extract 9.85 g Caffeine 0.15 g Dosage: use one to two vials of 25 cl per day.

  EXAMPLE 9 Preparations of approximately 1 liter of a composition were prepared to improve intestinal transit and waist circumference corresponding to the following weight formula: Liquid yogurt 98,800 g Tamarind pulp 6,500 g Papaya extract 2,500 g Green clay 3.000 g Vitamin B6 0.002 g Vitamin E 0.010 g Dosage: use 100 ml in the morning or in the evening. 25

EXAMPLE 12

  Combination of the synergistic composition of Example 4 with 100 grams of protein-enriched pasta for lunch and dinner and unlimited green vegetables to reduce weight. 30

EXAMPLE 13

  Combination of the synergistic composition of Example 1 with a slimming gel corresponding to the formula: Percentage by weight Caffeine 2.0 Extract of Centella asiatica 2.0 Extract of Mimosa tenuiflora 1.5 Extract of Azadirachta indica 0.6 Extract of Garcinia cambogia 5.5 Excipients qs 100% Dosage: apply a nut of product morning and evening and penetrate with a light massage.

  TOLERANCE STUDIES: Acute toxicity studies in rats carried out according to OECD guidelines No. 401 under the guise of quality assurance show that the preparations used orally presented as an example are non-toxic at a dose of 5 g / kg. Studies of local skin tolerance and eye tolerance in rabbits carried out according to the official method for the assessment of skin surface aggressiveness (Order of May 11, 1993) and the official method for the evaluation of eye irritation ( Decree of June 9, 1992) under the guise of quality assurance show that the preparation presented in example n013 is non-irritant by local route and slightly irritant by ocular route. 20

PHARMACOLOGICAL STUDIES

  Search for combinations of agents capable of stimulating cell metabolism combined with plant extracts.

  To assess the effect of vitamins, minerals and trace elements on the metabolic activity of cells, the cell culture model with accumulation of intracellular lipids was used. In vitro cultures of smooth muscle cells were prepared on 1 cm diameter plastic coverslips according to the method described by Shrivastava et al. (Meth. Find. Exp. Clin. Pharmacol., 15, 345-350, 1993.). A monolayer culture at 5060% confluence was obtained in 4 days. The hyperlipidemic rabbit serum was added to the culture medium, the cells were incubated at 37 ° C. and 5% CO 2 for 48 hours and the deposits of intracytoplasmic lipids were quantified. The culture medium was then replaced by a white medium predefined with 1% fetal calf serum but containing no vitamins, minerals and trace elements.

  The desired concentrations and combinations of vitamins, minerals and trace elements were prepared in the predefined white medium by dissolving the substances tested in the medium and replacing the original culture medium with the medium containing the products to be tested.

  The cytotoxicity of each vitamin, mineral or trace element has been previously determined. None of the concentrations used were cytotoxic. The concentration of product tested represented 1/100 of the Recommended Daily Allowance 10 (RDA) per milliliter of culture medium.

  The products tested were: vitamin A (beta carotene), vitamin Bl (thiamine hydrochloride), vitamin B2 (riboflavin), Vitamin B3 or PP (nicotinamide), vitamin B5 (pantothenic acid), vitamin B6 (pyridoxine hydrochloride) ), Vitamin B8 or H, vitamin B9 (folic acid), vitamin B12 (cyanocobalamin), vitamin C (ascorbic acid), vitamin E (tocopherol acetate), iron (iron gluconate), iodine (potassium iodide), potassium (potassium chloride), zinc (zinc oxide), copper (copper oxide), magnesium (magnesium oxide), calcium (calcium gluconate), selenium (sodium selenate). Only the elements which showed a certain activity during the preliminary selection stage were retained for the following experiments.

  Intracellular lipid deposits were evaluated 3 days after exposure to the products tested according to the method described by Shrivastava et al. (Meth. Find. Exp.

  Clin. Pharmacol., 15, 345-350, 1993.). The results were expressed as a percentage of average decrease in intracellular lipid deposits compared to the untreated control cultures considered to contain 100% lipid deposits. The decrease in intracellular lipids is directly proportional to the increase in the metabolic activity of cells.

  The following results were obtained with the products tested alone (Table 1), with the associations of two products (Table 2) and with the synergistic associations of several micronutrients and different drinks (Table 3).

  Table 1: Percentage decrease in intracellular lipid deposits with the products tested alone (n = 24).

  Element Concentration Tested in pg / ml (0.016% of RDA) Vitamin A 15 Vitamin B1 Vitamin B2 Vitamin B3 Vitamin B5 Vitamin B6 20 Vitamin B8 Vitamin B9 Vitamin B12 Vitamin C Vitamin E 25 Magnesium Calcium Iron Iodine Potassium 30 Zinc Copper Selenium 8.0 0.23 0.26 3.0 1.0 0.335 0.025 0.035 0.0001 20.0 3.33 66.67 178.0 18.64 0.03 24.49 3.11 0.42 0.013 Percentage decrease intracellular lipids 12.6 + 2.3 7.7 + 1.4 14.5 + 3.9 2.5 1.0 4.5 + 2.1 9.9 2.9 1.8 + 0.7 17 5.5 + 4.6 8.2 2.5 11.2 4.1 21.4 3.9 22.0 + 5.2 o0 6.3 2.7 o0 o0 16.3 + 4.4 24.3 3 6.2 24.4 + 5.3 These results show that the products tested alone 35 have no effect on reducing the accumulation of intracellular lipid waste except a slight effect for vitamin A, vitamin B2 , vitamin B9, vitamin E, magnesium, zinc, copper and selenium.

  Table 2: Percentage decrease in intracellular lipid deposits with combinations of two products (n = 24) Percentage decrease in intracellular lipid Vitamin A + vitamin B2 20.2 4.6 Vitamin A + vitamin B9 26.3 5.5 Vitamin A + vitamin E 29.2 i 6.7 Vitamin A + magnesium 26.1 4.4 Vitamin A + zinc 18.3 4.4 Vitamin A + copper 21.3 9.1 Vitamin A + selenium 27.2 6, 1 Vitamin B2 + vitamin B9 18.3 2.7 Vitamin B2 + vitamin E 20.0 4, 1 Vitamin B2 + magnesium 30.2 8.1 Vitamin B2 + zinc 21.1 6.6 Vitamin B2 + copper 23.3 7.3 Vitamin B9 + vitamin E 24.4 6.6 Vitamin B9 + magnesium 18.1 8.2 Vitamin B9 + zinc 18.4 3.2 Vitamin B9 + copper 22.6 4.9 Vitamin E + magnesium 25, 9 4.9 Vitamin E + zinc 26.6 7.6 Vitamin E + copper 17.7 2.7 Zinc + copper 16.6 4.9 Zinc + magnesium 21.9 7.2 Copper + magnesium 24.6 6, 2 Vitamin B2 + selenium 24.6 2.7 Vitamin E + selenium 22.2 7.8 Magnesium + selenium 23, 7 8.2 These results show that the combination of two vital elements does not greatly improve the elimination of intracellular lipids. The activity is not additive.

  Table 3: Percentage decrease in intracellular lipid deposits with synergistic associations of several micronutrients (n = 24) possibly in combination with plant extracts and different drinks. (vitamin A = A, vitamin B2 = B2 and so on for vitamins; the international symbols of the periodic table of the elements designate the corresponding minerals and trace elements).

  A + B2 + B9 + EA + B2 + Mg + Zn 15 A + B5 + B6 + B8 A + Fe + I + K B9 + Fe + Mg + Zn A + B2 + Cu + Se A + B2 + Mg + Se 20 B5 + B6 + Cu + Se B2 + B9 + Mg + Zn B2 + B9 + Zn + Se B2 + B9 + Mg + Se B2 + B9 + Cu + Se 25 B2 + Mg + Cu + Se B5 + B6 + B8 + Fe + IA + B2 + Mg + Cu B2 + B9 + E + Zn D + E + Zn + Si E + Ca + Fe + Cr A + B2 + B9 + E + Mg + Zn + Cu + Se A + B2 + B9 + Mg + Zn + Cu + Se B2 + B9 + E + Mg + Zn + Cu + Se A + E + Mg + Zn + Cu + Se 35 A + E + Zn + Cu + Se E + Mg + Zn + Cu + Se A + B2 + Mg + Cu + caffeine (0.01%) Percentage decrease in intracellular lipids 16. 4 6.9 60.0 9.3 12.4 7.6 5.3 4.3 21.2 8.4 58.4 8.8 71.2 11.4 cytotoxic 58.8 11.4 67.3 9.9 66.3 13.2 70.4 + 8.2 24.9 6.7 14.6 _ 7.2 66.3 14.6 32.6 14.6 42.0 9.0 26.4 + 8.8 79.2 + 12.6 62.1 11. 6 71.6 12.0 61.6 + 9.9 51.4 _ 13.0 30.1 8.8 74.4 _ 14.6 A + B2 + Mg + Cu + Soybean phytoestrogens (10%) Caffeine alone (0.01%) Guarana extract alone (0.03%) Green tea only (co extract now 3% caffeine) Brewer's yeast extract (4% filtered at 0.22p) Caffeine (0.01%) + A + B2 + B9 15 + E + Mg + Zn + Cu + Se Guarana extract (0.03%) + A + B2 + B9 + E + Mg + Zn + Cu + Se Green tea (3% caffeine)%) + A + B2 + B9 + E + Mg + Zn + Cu + Se Brewer's yeast extract (4% filtered at 0.22 p) + A + B2 + B9 + E 25 Brewer's yeast extract (4% filtered at 0.22 b) + B2 + Mg + Cu + Se Brewer's yeast extract (4% filtered at 0.22 b) + A + B2 + B9 + E + Mg + Zn + Cu + Se Orange juice only Pineapple juice only 35 Apple juice only Pineapple juice + Guarana extract 83.4 26.4 41.2 17.3 + 11.3 + 28.11 14.7 3.7 22.3 + 0.2 78.1 + 6.8 90.2 + 13.2 81.7 14.5 62.4 8.6 21.9 12.4 82.9 24.2 + 19.2 + 28.4 86.9 9.8 6.7 8.6 6.3 6.2 Milk + Guarana extract + Chicory inulin 63.4 9.6 Yeasts + Guarana extract 59.3 10.2 Pineapple juice + Yeasts + Guarana extract 87.1 8.6 Pineapple juice + A + B2 + B9 + E + Mg + Zn + Cu + Se + Guarana extract 89.6 4.6 The results show that to eliminate intracellular lipids, it is essential to combine at least two vitamins among vitamin A, vitamin B2, vitamin B9, vitamin E and at least two minerals or trace elements among magnesium, zinc, copper and selenium or to use, to replace them, a fruit juice naturally rich in these vital elements. The best results are obtained by combining these 8 elements.

  Plant extracts alone help decrease intracellular lipid deposition but the effects are not very pronounced. Their combination with at least two vitamins and two minerals produces a synergistic effect. The yeast extract rich in minerals and trace elements used alone or in combination with other minerals and trace elements has no effect but the addition of vitamins greatly increases the activity, indicating that the minerals and trace elements alone have no marked effect on cell metabolism.

  These results also show that vitamins A, B2, B9, 30 E, magnesium and zinc, copper and selenium are essential to improve the activity of plant extracts. Fruit juices or milk rich in vitamins or yeasts rich in minerals and trace elements make it possible to greatly improve the activity of plant extracts.

  Pain-relieving effect of Salix alba in rats Four groups of 12 rats were subjected to the hot plate test. The tail of the animals was placed on a plate 5 gradually heated to a moderate temperature.

  When the feeling of heat becomes unbearable for the animals, they naturally remove the tail. The longer the tail retention time, the less the pain sensation. This test measures the pain-relieving effect of therapeutic agents. The following results have been obtained.

  Treatment group Percentage reduction in pain sensation 0% Group 1 Group 2 20 Group 3 none mineral vitamins trace elements Salix alba (500 mg / kg) 1.5% -12.6% (p <0.05) Group 4 25 Group 5 Salix alba (500 mg / kg) + mineral vitamins trace elements Salix alba (500 mg / kg) + fruit juice rich in mineral vitamins -34.2% (p <0.001) -39.7% (p <0.001) These results clearly show that the pain-relieving effect of Salix alba has been improved when it is combined with vitamins and minerals or with a drink rich in vitamins, minerals and trace elements.

  Likewise, experiments carried out in vivo have shown that certain compositions based on plant extracts help to reduce weight but that their effect can be extremely improved when these compositions are used in the same proportions in a fruit juice.

  Effect of the association of slimming plant extracts on weight reduction in rats Twelve rats per group (6 males and 6 females) aged 8 weeks received food in the form of granules enriched with 1% fat ( cholesterol) for 4 weeks. One group of rats received a specific combination of plant extracts and everyday drinking water while the other groups received the same treatment mixed with different fruit juices rich in vitamins, minerals and trace elements. Two other control groups receiving only water or fruit juices without plant extracts were formed. The percentage increase in body mass was measured after 4 weeks of treatment. The average increase in weight was as follows Water alone + 112% Fruit juice alone + 88% Plant extract + fruit juice + 43% Plant extract + Water + 101% These results clearly show that the composition based on slimming plants used alone or with running water influences body weight gain less than when used in combination with fruit juices rich in vitamins, minerals and trace elements in rats receiving enriched food in fat.

CLINICAL STUDIES

  Study nl: research on the slimming effect of synergistic compositions of vitamins, minerals and trace elements, 5 plant extracts and fruit juices, and a high-protein diet Adult male volunteers (approximately 35%) or female (about 65%) with a body mass index of 20% higher than normal were selected to evaluate the effect of different diets on the evolution of body weight over a period of 30 days. The weight of the volunteers was also evaluated 3 months after the start date of the study. The effect on different parameters of well-being was evaluated using score scales ranging from 0 to 4. Three groups studied under identical conditions were formed for the study conducted by a research institute accredited by the Ministry of the research. Group 1: one capsule per day containing 50% of the RDI in vitamin A, B2, B9, E, magnesium, zinc, copper and selenium. Group 2 composition combining plant extracts according to Example 4 based on pineapple juice.

  Group 3: combination of a dietary supplement of vitamins, minerals and trace elements associated with a diet containing unlimited green vegetables, and 100 grams of high protein pasta to consume for lunch and dinner.

  The following average results were obtained Number of Weight Loss Average volunteer well-being index days (1 to 4) HFHF Average Group 1: 8 30 -2.22 -2.61 -2.47 3.21 Group 2: 9 18 -3.77 -3.92 - 3.84 3. 52 Group 3: 4 12 -2.10 -2.93 -2.62 3.11 These results show that the association of 50% of RDA in vitamin A, vitamin B2, vitamin B9, vitamin E, magnesium, zinc, copper and selenium, decreases weight in men and women (average of -2.47 kg in 30 days) by accelerating cell metabolism. An improvement in the results is obtained when the plant extracts are combined with a fruit juice rich in vitamins, minerals and trace elements.

  Results can also be obtained with diets but the reduction in weight obtained is not always significant, which shows that the greatest part of the weight loss observed is due to the synergistic composition of vitamins (A, B2, B9 , E), minerals and trace elements (magnesium, zinc, copper, selenium).

  Furthermore, the weight of the volunteers who used a synergistic composition combining plant extracts with fruit juice containing vitamins, minerals and trace elements remained stable at the end of the three months of the observation period, which was not the case for subjects using a low-calorie diet alone. This observation shows that the use of a synergistic composition also makes it possible to stabilize the weight in order to prevent the rebound effect observed at the end of a diet.

  Effect on well-being parameters In the subjects studied, the well-being index was greatly increased with the synergistic composition combining plant extracts and fruit juices rich in vitamins, minerals and trace elements and which improves remarkably the parameters for evaluating stress, sleep, physical shape and fatigue, which shows that the synergistic composition used has a favorable action on general health.

  Study n02: Research on the effect of plant extracts used alone or in combination with fruit juices on waist size, intestinal transit and the wellbeing index in healthy volunteers The same results were obtained with a specific composition for reducing the waist circumference according to Example 5 which was used for 20 days by volunteers during a clinical study. Five groups were formed: 10 the first group received the composition according to Example 5 with apple juice, the second group received the composition according to Example 5 with mineral water, the third group received the same extracts alone while two other control groups received fruit juice or mineral water alone. The following 15 results have been obtained: Treatment Decrease Intestinal belly transit score Group 1 plant extracts + fruit juice - 3.83 cm +6 Group 2 plant extracts + mineral water -2.46 cm +5 Group 3 plant extracts Only -2.30 cm +3 Group 4 fruit juice alone -0.42 cm +1 Group 5 mineral water alone -0.64 cm 0 Wellbeing index (0-10) Group 1 +8 Group 2 +7 Group 3 +3 Group 4 +3 Group 5 +0 These results show that the decrease in waist size, the intestinal transit score and the well-being index are improved when the plant extracts are combined with water or a juice fruit rich in vitamins, minerals and trace elements.

  Study No. 03: A 6-month clinical study was carried out with two groups of 20 volunteers for 6 months. The first group used two bottles per day of the composition according to example No. 08 10 based on alcohol-free beer and the second control group received the same alcohol-free beer without caffeine or plant extracts (placebo). The following results were obtained: Treatment placebo group Group receiving the composition according to Example 8 Effect on weight (kg) after 6 months +1.5 0.5 kg These results show that after 6 months, the group receiving the composition according to example n08 lost weight (-0.5 kg on average) while the volunteers in the group who used the placebo saw their weight increase (+1.5 kg on average).

Claims (11)

  1. Use of a non-solid synergistic composition by the oral route to improve the effectiveness of plant extracts, characterized in that it comprises plant extracts and at least two vitamins chosen from vitamin A, vitamin B2, vitamin B9, vitamin E with at least two minerals or trace elements chosen from magnesium, zinc, copper and selenium 10 to stimulate cell metabolism.   2. Use of a synergistic composition according to claim 1 characterized in that it comprises plant extracts and drinks rich or enriched with at least two vitamins chosen from vitamin A, vitamin B2, vitamin B9, vitamin E with at least two minerals or trace elements chosen from magnesium, zinc, copper and selenium to stimulate cell metabolism. 20 3. Use of a composition according to claims i to 2 with plant extracts which are known to reduce weight to treat overweight.   4. Use of a composition according to claims 1 to 2 with plant extracts which are known to improve intestinal transit to reduce the abdominal circumference or to obtain a flatter stomach. 5. Use of a composition according to claims 1 to 2 with plant extracts which are known to reduce stress or improve vitality.   6. Use of a synergistic composition according to claim 1 characterized in that it comprises plant extracts and drinks such as milk, mineral waters, or fermented drinks such as beer or cider.   7. Use of a synergistic composition according to claims 1 to 6 in combination with a low-calorie, high-protein diet or stimulating intestinal transit.   8. Use of a synergistic composition according to claims 1 to 6 in combination with a cosmetic product. 9. Use of a synergistic composition according to claims 1 to 6 in combination with a physical exercise device or a muscle electrostimulation device. 10. A combination of compositions as defined in one of claims 1 to 6 and an acceptable excipient in food.   11. A combination of compositions as defined in one of claims 1 to 6 as a dietetic, nutritional or pharmaceutical or cosmetic product.