FR2432710A1 - The illumination and capture dark field and method of use - Google Patents

The illumination and capture dark field and method of use

Info

Publication number
FR2432710A1
FR2432710A1 FR7919460A FR7919460A FR2432710A1 FR 2432710 A1 FR2432710 A1 FR 2432710A1 FR 7919460 A FR7919460 A FR 7919460A FR 7919460 A FR7919460 A FR 7919460A FR 2432710 A1 FR2432710 A1 FR 2432710A1
Authority
FR
France
Prior art keywords
gt
lt
illumination
particles
fluorescent light
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7919460A
Other languages
French (fr)
Other versions
FR2432710B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coulter Electronics Inc
Original Assignee
Coulter Electronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US05/929,267 priority Critical patent/US4199686A/en
Application filed by Coulter Electronics Inc filed Critical Coulter Electronics Inc
Publication of FR2432710A1 publication Critical patent/FR2432710A1/en
Application granted granted Critical
Publication of FR2432710B1 publication Critical patent/FR2432710B1/fr
Application status is Expired legal-status Critical

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N2021/6463Optics
    • G01N2021/6469Cavity, e.g. ellipsoid

Abstract

<P>La présente invention concerne un dispositif d'éclairement et de captation ainsi qu'une méthode pour l'analyse de fluorescence, où l'éclairement d'un courant de particules produit de la lumière fluorescente. <P> This invention relates to an illumination and capturing device and a method for fluorescence analysis, where the illumination of a product stream of particles of the fluorescent light. </P><P>Selon l'invention, un réflecteur 12, ayant une surface concave 14 avec un premier foyer 16 disposé dans le courant des particules, réfléchit un rayonnement d'éclairement organisé, ce rayonnement convergeant sur le premier foyer 16 pour stimuler les particules pour qu'elles produisent la lumière fluorescente; </ P> <P> According to the invention, a reflector 12 having a concave surface 14 with a first focus 16 disposed in the stream of particles, reflects an organized illumination radiation, this radiation converging on the first focal point 16 to stimulate particles to produce fluorescent light; la lumière fluorescente émane du premier foyer 16, dont une partie est réfléchie par la surface 14; the fluorescent light emanating from the first focal point 16, part of which is reflected by the surface 14; cette lumière fluorescente qui est focalisée sur le second foyer 18 ne contient pas de rayonnement d'éclairement et est recueillie pour une détection et un traitement subséquents. this fluorescent light that is focused on the second focal point 18 contains no illumination radiation and is collected for detection and subsequent treatment. </P><P>L'invention s'applique notamment à la détection des particules.</P> </ P> <P> The invention is particularly applicable to the detection of particles. </ P>
FR7919460A 1978-07-31 1979-07-27 Expired FR2432710B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US05/929,267 US4199686A (en) 1978-07-31 1978-07-31 Dark field illuminator and collector apparatus and method

Publications (2)

Publication Number Publication Date
FR2432710A1 true FR2432710A1 (en) 1980-02-29
FR2432710B1 FR2432710B1 (en) 1983-02-18

Family

ID=25457577

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7919460A Expired FR2432710B1 (en) 1978-07-31 1979-07-27

Country Status (6)

Country Link
US (1) US4199686A (en)
JP (1) JPS5522189A (en)
CA (1) CA1113763A (en)
DE (1) DE2930593A1 (en)
FR (1) FR2432710B1 (en)
GB (1) GB2027193B (en)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8102772L (en) * 1981-05-04 1982-11-05 Sven Staffan Folestad Method for laser-induced fluoriscensdetektering and apparatus for performing the method
US4592726A (en) * 1983-01-03 1986-06-03 Herbert Brilliant Diagnostic mirror and light filter
CA1228748A (en) * 1983-12-24 1987-11-03 Hans Oetliker Method and apparatus for guiding and collecting light in photometry or the like
DE3922153C2 (en) * 1989-07-06 1993-08-12 Hella Kg Hueck & Co, 4780 Lippstadt, De
US5018866A (en) * 1989-09-12 1991-05-28 Packard Instrument Company Method and apparatus for performing high sensitivity fluorescence measurements
US5203339A (en) * 1991-06-28 1993-04-20 The Government Of The United States Of America As Represented By The Secretary Of The Department Health And Human Services Method and apparatus for imaging a physical parameter in turbid media using diffuse waves
US5484571A (en) * 1991-10-08 1996-01-16 Beckman Instruments, Inc. Enhanced fluorescence detection of samples in capillary column
US5430541A (en) * 1993-01-12 1995-07-04 Applied Biosystems Inc. High efficiency fluorescence flow cell for capillary liquid chromatography or capillary electrophoresis
EP2264428B1 (en) * 1997-01-31 2017-05-03 Xy, Llc Optical apparatus with focussing reflector for converging radiation onto a flow of particles
DE19735119C2 (en) * 1997-08-13 2000-05-18 Deutsches Krebsforsch A method for fluorescence correlation spectroscopy, insbesbesondere for multicolour fluorescence correlation spectroscopy and apparatus for performing the method
US7198939B2 (en) * 2000-01-28 2007-04-03 Agilent Technologies, Inc. Apparatus for interrogating an addressable array
US20070059760A1 (en) * 2002-02-21 2007-03-15 Dorsel Andreas N Multi-featured arrays with reflective coating
US7300798B2 (en) * 2001-10-18 2007-11-27 Agilent Technologies, Inc. Chemical arrays
US7371582B2 (en) * 2002-01-23 2008-05-13 Boditechmed Inc. Lateral flow quantitative assay method and strip and laser-induced fluorescence detection device therefor
US6791690B2 (en) * 2002-04-30 2004-09-14 Agilent Technologies, Inc. Reading dry chemical arrays
JP2004343082A (en) * 2003-04-17 2004-12-02 Asml Netherlands Bv Lithographic projection apparatus with condenser including concave face and convex face
GB2438332B (en) * 2003-10-24 2008-04-09 Biotrace Ltd Bioluminescence monitor
GB0324784D0 (en) 2003-10-24 2003-11-26 Biotrace Ltd Bioluminescence monitor
FR2873438B1 (en) * 2004-07-23 2006-11-17 Tda Armements Sas Soc Par Acti Method and load activation system of a munition, munition also equipped with an activation device has high accuracy and system of neutralizing a target
US7616311B2 (en) 2005-05-02 2009-11-10 Jmar Llc Systems and methods for a multiple angle light scattering (MALS) instrument having two-dimensional detector array
US7564551B2 (en) * 2005-05-02 2009-07-21 Jmar Technologies, Inc. Systems and methods for a high capture angle, multiple angle light scattering (MALS) instrument
US7551279B2 (en) * 2005-09-19 2009-06-23 Jmar Technologies, Inc. Systems and methods for detecting normal levels of bacteria in water using a multiple angle light scattering (MALS) instrument
JP2007071794A (en) * 2005-09-09 2007-03-22 Rion Co Ltd Particle detector
WO2008021834A2 (en) * 2006-08-11 2008-02-21 The Regents Of The University Of California High-resolution microscope using optical amplification
JP4857253B2 (en) * 2007-12-07 2012-01-18 株式会社日立製作所 Image display device
CN102004089B (en) * 2010-11-19 2012-11-21 山东省医药生物技术研究中心 Device and method for detecting property of traditional Chinese medicine

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3248551A (en) * 1962-10-22 1966-04-26 Joseph C Frommer Optical arrangement for sensing very small particles
US3457407A (en) * 1966-07-27 1969-07-22 Us Navy Apparatus for quantitatively detecting foreign particles present in atmospheric air
US3989381A (en) * 1975-05-05 1976-11-02 Coulter Electronics, Inc. Optical chamber with spherical reflective portion and apparatus employing same
US4099872A (en) * 1975-12-11 1978-07-11 White John U Fluorescence spectrophotometer

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2661658A (en) * 1948-07-19 1953-12-08 Ass Elect Ind Optical system for increasing the working distances of microscope objectives
US3622796A (en) * 1969-11-13 1971-11-23 Te Co The Selective collector for the wide-angle portion of a radiation beam
CA962084A (en) * 1971-01-29 1975-02-04 Coulter Electronics Optical aerosol counter
US3946239A (en) * 1975-01-24 1976-03-23 The United States Of America As Represented By The United Energy Research And Development Administration Ellipsoidal cell flow system
CA1029981A (en) * 1975-07-24 1978-04-25 Parr A. Tate Device to detect small spots of fluorescent material

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3248551A (en) * 1962-10-22 1966-04-26 Joseph C Frommer Optical arrangement for sensing very small particles
US3457407A (en) * 1966-07-27 1969-07-22 Us Navy Apparatus for quantitatively detecting foreign particles present in atmospheric air
US3989381A (en) * 1975-05-05 1976-11-02 Coulter Electronics, Inc. Optical chamber with spherical reflective portion and apparatus employing same
US4099872A (en) * 1975-12-11 1978-07-11 White John U Fluorescence spectrophotometer

Also Published As

Publication number Publication date
DE2930593A1 (en) 1980-02-28
GB2027193B (en) 1983-03-23
GB2027193A (en) 1980-02-13
CA1113763A1 (en)
CA1113763A (en) 1981-12-08
FR2432710B1 (en) 1983-02-18
JPS5522189A (en) 1980-02-16
US4199686A (en) 1980-04-22

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