ES8602120A1 - Un procedimiento para preparar un vector que comprende la region reguladora arg3 de levadura y un sitio de restriccion proximo a ella - Google Patents

Un procedimiento para preparar un vector que comprende la region reguladora arg3 de levadura y un sitio de restriccion proximo a ella

Info

Publication number
ES8602120A1
ES8602120A1 ES532731A ES532731A ES8602120A1 ES 8602120 A1 ES8602120 A1 ES 8602120A1 ES 532731 A ES532731 A ES 532731A ES 532731 A ES532731 A ES 532731A ES 8602120 A1 ES8602120 A1 ES 8602120A1
Authority
ES
Spain
Prior art keywords
hepatitis
preparation
recombinant dna
dna molecules
surface antigen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES532731A
Other languages
English (en)
Other versions
ES532731A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline RIT
Original Assignee
SmithKline RIT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline RIT filed Critical SmithKline RIT
Publication of ES8602120A1 publication Critical patent/ES8602120A1/es
Publication of ES532731A0 publication Critical patent/ES532731A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Communicable Diseases (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Oncology (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Public Health (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

PROCEDIMIENTO PARA PREPARAR UN VECTOR QUE COMPRENDE LA REGION REGULADORA ARG3 DE LEVADURA Y UN SITIO DE RESTRICCION PROXIMO A ELLA. COMPRENDE: FUSIONAR LA REGION REGULADORA, QUE ES UN FRAGMENTO DE 1.480 PARES DE BASES DE PMC 200 QUE CONSTA DE UNA EXTENSION DE ECORI EN UNA EXTREMIDAD Y QUE TERMINA EN UN RESIDUO 3T EN LA OTRA EXTREMIDAD QUE ES DE EXTREMOS ROMOS A UN FRAGMENTO DE XBAL-ECORI A 5.700 PARES DE BASES DE PMC 200, DESPUES DE REPARACION POR POLIMERASA DEL SITIO XBAL DEL FRAGMENTO DE 5.700 PARES DE BASES.
ES532731A 1982-09-08 1984-05-23 Un procedimiento para preparar un vector que comprende la region reguladora arg3 de levadura y un sitio de restriccion proximo a ella Granted ES532731A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US41578982A 1982-09-08 1982-09-08

Publications (2)

Publication Number Publication Date
ES8602120A1 true ES8602120A1 (es) 1985-11-01
ES532731A0 ES532731A0 (es) 1985-11-01

Family

ID=23647197

Family Applications (4)

Application Number Title Priority Date Filing Date
ES525439A Granted ES525439A0 (es) 1982-09-08 1983-09-07 Un procedimiento para preparar una molecula de dna recombinante
ES532732A Granted ES532732A0 (es) 1982-09-08 1984-05-23 Un procedimiento para preparar una vacuna para el virus de la hepatitis b
ES532730A Expired ES8600938A1 (es) 1982-09-08 1984-05-23 Un procedimiento para preparar el antigeno de superficie de la hepatitis b
ES532731A Granted ES532731A0 (es) 1982-09-08 1984-05-23 Un procedimiento para preparar un vector que comprende la region reguladora arg3 de levadura y un sitio de restriccion proximo a ella

Family Applications Before (3)

Application Number Title Priority Date Filing Date
ES525439A Granted ES525439A0 (es) 1982-09-08 1983-09-07 Un procedimiento para preparar una molecula de dna recombinante
ES532732A Granted ES532732A0 (es) 1982-09-08 1984-05-23 Un procedimiento para preparar una vacuna para el virus de la hepatitis b
ES532730A Expired ES8600938A1 (es) 1982-09-08 1984-05-23 Un procedimiento para preparar el antigeno de superficie de la hepatitis b

Country Status (22)

Country Link
EP (1) EP0106828B1 (es)
JP (5) JPS5971694A (es)
KR (1) KR840006014A (es)
AT (1) ATE91718T1 (es)
AU (4) AU584580B2 (es)
CA (1) CA1222707A (es)
CY (1) CY1932A (es)
DE (1) DE3382704T2 (es)
DK (1) DK406483A (es)
ES (4) ES525439A0 (es)
FI (1) FI833205A (es)
GR (1) GR78982B (es)
HK (1) HK1004570A1 (es)
HU (1) HU196625B (es)
IE (1) IE60387B1 (es)
IL (1) IL69202A (es)
MA (1) MA19895A1 (es)
MY (1) MY102920A (es)
NO (1) NO833198L (es)
NZ (1) NZ204952A (es)
PT (1) PT77265B (es)
ZA (1) ZA836658B (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5931799A (ja) * 1982-08-16 1984-02-20 Science & Tech Agency Ｂ型肝炎ウイルス遺伝子を組込んだ組換えプラスミド
JPS5936699A (ja) * 1982-08-20 1984-02-28 Chemo Sero Therapeut Res Inst シヤトルベクタ−
WO1986001534A1 (en) * 1984-09-04 1986-03-13 Takeda Chemical Industries, Ltd. Recombinant dna and its use
WO1986007384A1 (en) * 1985-06-03 1986-12-18 Takeda Chemical Industries, Ltd. Process for preparing hapatitis b virus surface antigen p31
WO1986003411A1 (en) * 1984-12-12 1986-06-19 Takeda Chemical Industries, Ltd. Process for preparing novel protein
US4683294A (en) * 1985-04-03 1987-07-28 Smith Kline Rit, S.A. Process for the extraction and purification of proteins from culture media producing them
DE3677851D1 (de) * 1985-08-05 1991-04-11 Merck & Co Inc Verfahren zur steigerung der produktion von rekombinantem protein in hefearten der gattung saccharomyces.
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
AP56A (en) * 1987-01-30 1989-09-26 Smithkline Biologicals S A Hepatitis B virus surface antigens and hybrid antigehs containing them.
DE3883596T2 (de) * 1987-03-09 1994-02-17 Merck & Co Inc Reinigung von rekombiniertem Hepatitis-B-Oberflächen-Antigen.
NZ229260A (en) * 1988-06-03 1994-02-25 Merck & Co Inc Hepatitis b virus, expression cassette for pre-s domain, host cells and
US4962656A (en) * 1989-06-30 1990-10-16 The United States Of America As Represented By The United States Department Of Energy Control and monitoring method and system for electromagnetic forming process
LT3988B (en) 1992-02-17 1996-06-25 Fermentas Biotech Inst Recombinant plasmides pfs19, pfps2-48 and pjlfds1 codingsynthesis of human hepatite b of surfice virus antigenes, methods fof producing thereof
TW340132B (en) * 1994-10-20 1998-09-11 Ibm Structure for use as an electrical interconnection means and process for preparing the same
JP4028850B2 (ja) * 2004-02-04 2007-12-26 株式会社名機製作所 ディスク基板の成形用金型

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
LU88257I2 (es) * 1978-12-22 1994-02-03
NZ199722A (en) * 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
GR76274B (es) * 1981-08-04 1984-08-04 Univ California
NZ201705A (en) * 1981-08-31 1986-03-14 Genentech Inc Recombinant dna method for production of hepatitis b surface antigen in yeast
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides

Also Published As

Publication number Publication date
IL69202A (en) 1991-08-16
AU1675083A (en) 1984-03-15
HK1004570A1 (en) 1998-11-27
ES532730A0 (es) 1985-10-16
AU1569288A (en) 1988-07-28
ES8600938A1 (es) 1985-10-16
ES8506350A1 (es) 1985-07-01
ES8600939A1 (es) 1985-10-16
DK406483A (da) 1984-03-09
KR840006014A (ko) 1984-11-21
JPH02464A (ja) 1990-01-05
NZ204952A (en) 1986-05-09
FI833205A0 (fi) 1983-09-07
EP0106828A2 (fr) 1984-04-25
EP0106828B1 (fr) 1993-07-21
FI833205A (fi) 1984-03-09
JP2522825B2 (ja) 1996-08-07
JPH02116A (ja) 1990-01-05
JP2702899B2 (ja) 1998-01-26
EP0106828A3 (en) 1985-11-21
IE60387B1 (en) 1994-07-13
AU584580B2 (en) 1989-06-01
DE3382704D1 (de) 1993-08-26
ES532731A0 (es) 1985-11-01
ES525439A0 (es) 1985-07-01
ATE91718T1 (de) 1993-08-15
CA1222707A (en) 1987-06-09
IE832099L (en) 1984-03-08
DE3382704T2 (de) 1994-02-10
AU9001491A (en) 1992-02-27
PT77265B (en) 1986-02-04
AU3131889A (en) 1989-07-20
MY102920A (en) 1993-03-31
ES532732A0 (es) 1985-10-16
NO833198L (no) 1984-03-09
PT77265A (en) 1983-09-01
MA19895A1 (fr) 1984-04-01
DK406483D0 (da) 1983-09-07
GR78982B (es) 1984-10-02
HU196625B (en) 1988-12-28
CY1932A (en) 1983-09-06
ZA836658B (en) 1984-06-27
JPH08317796A (ja) 1996-12-03
JPS5971694A (ja) 1984-04-23
JPH02231084A (ja) 1990-09-13

Similar Documents

Publication Publication Date Title
ES8602120A1 (es) Un procedimiento para preparar un vector que comprende la region reguladora arg3 de levadura y un sitio de restriccion proximo a ella
AP8600035A0 (en) Hepatitis B surface antigen formed by recombinant DNA Techniques, vaccines, diagnostics, cell lines and methods of forming same.
EP0120551A3 (en) Yeast expression systems with vectors having gapdh or pyk promoters, and synthesis of foreign proteins
EP0291586A3 (en) Recombinant hepatitis b surface antigen and vaccine containing it
ES8105388A1 (es) Un metodo para mantener, replicar y expresar por lo menos una porcion del genoma de un virus n.p.
IL90035A (en) Synthetic multimeric hepatitis b virus vaccine including both t cell and b cell determinants
EP0171908A3 (en) Hepatitis b virus surface antigen and production thereof
IL69423A0 (en) Recombinant plasmid inserted with hepatitis b virus gene,yeast transformed with said recombinant plasmid and production of hepatitis b virus surface antigen
EP0105149A3 (en) Recombinant plasmid containing hepatitis b virus gene, yeast transformed with said recombinant plasmid, and production of hepatitis b virus surface antigen
EP0068719A3 (en) Deoxyribonucleic acids, their production and use
EP0152030A3 (en) Adult t cell leukemia virus antigen polypeptide
FI861559A (fi) Menetelmä hepatitis B-viruksen pinta-antigeenin PreS1-PreS2-S-proteiinin koodaavan alueen koodaamia proteiineja käsittävän proteiinin valmistamiseksi sekä menetelmässä käytettävät yhdistelmä-DNA-vektorit ja -kontameerit sekä nisäkässolut
ES8602935A1 (es) Un vector reversible
JPS6467191A (en) Recombinant dna plasmid having integrated hepatitis b virus gene, transformed animal cell, hepatitis b viral surface antigen protein and production thereof
SU1380209A1 (ru) РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК PUR 291-HAV 22, КОДИРУЮЩАЯ СИНТЕЗ ГИБРИДНОГО ПОЛИПЕПТИДА АНТИГЕННЫХ ДЕТЕРМИНАНТ ВИРУСА ГЕПАТИТА А С β -ГАЛАКТОЗИДАЗОЙ

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970519