ES441738A1 - Method for the enzyme immunoassay of progesterone - Google Patents

Method for the enzyme immunoassay of progesterone

Info

Publication number
ES441738A1
ES441738A1 ES441738A ES441738A ES441738A1 ES 441738 A1 ES441738 A1 ES 441738A1 ES 441738 A ES441738 A ES 441738A ES 441738 A ES441738 A ES 441738A ES 441738 A1 ES441738 A1 ES 441738A1
Authority
ES
Spain
Prior art keywords
progesterone
called
molecules
antibody
antiprogesterone
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES441738A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur de Lille
Original Assignee
Institut Pasteur de Lille
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur de Lille filed Critical Institut Pasteur de Lille
Publication of ES441738A1 publication Critical patent/ES441738A1/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/743—Steroid hormones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

An immunoenzymatic dosing procedure for progesterone, which uses competitive reactions in liquid medium of the cited progesterone molecules, molecules called Ag, and progesterone molecules coupled to an enzyme, β-D-galactosidase, molecules called Ag-GZ, against spots equals of an antibody consisting of antiprogesterone serum called Ab1, and a precipitation of the soluble complexes obtained by a second insoluble antibody called Ab2, a reaction that leads to a residue that contains all the antibody populations and to a supernatant liquid that contains the fractions of Ag and Ag-GZ that have not reacted with Ab1, characterized by using the same progesterone derivative, 11-α-hemisuccinate of progesterone, for coupling with β-galactosidase and for the production of antiprogesterone serum. (Machine-translation by Google Translate, not legally binding)
ES441738A 1974-10-14 1975-10-13 Method for the enzyme immunoassay of progesterone Expired ES441738A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7434519A FR2288312A1 (en) 1974-10-14 1974-10-14 PROGESTERONE IMMUNOENZYMATIC ASSAY PROCESS

Publications (1)

Publication Number Publication Date
ES441738A1 true ES441738A1 (en) 1977-03-16

Family

ID=9144070

Family Applications (1)

Application Number Title Priority Date Filing Date
ES441738A Expired ES441738A1 (en) 1974-10-14 1975-10-13 Method for the enzyme immunoassay of progesterone

Country Status (8)

Country Link
JP (1) JPS5191325A (en)
BE (1) BE834248A (en)
CA (1) CA1071535A (en)
DE (1) DE2545980A1 (en)
ES (1) ES441738A1 (en)
FR (1) FR2288312A1 (en)
GB (1) GB1531974A (en)
NL (1) NL7512025A (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6111664A (en) 1984-06-28 1986-01-20 Ono Pharmaceut Co Ltd Reagent composition for enzyme immunomeasurement of prostaglandin and measuring method of prostaglandin using said composition
CH670709A5 (en) * 1986-04-24 1989-06-30 Univ Moskovsk

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3637711A (en) * 1968-03-25 1972-01-25 Ashland Oil Inc Beta-alkenyl substituted 8-hydroxyquinolines
NL154599B (en) * 1970-12-28 1977-09-15 Organon Nv PROCEDURE FOR DETERMINING AND DETERMINING SPECIFIC BINDING PROTEINS AND THEIR CORRESPONDING BINDABLE SUBSTANCES, AND TEST PACKAGING.
NL171930C (en) * 1972-05-11 1983-06-01 Akzo Nv METHOD FOR DETERMINING AND DETERMINING BITES AND TEST PACKAGING.

Also Published As

Publication number Publication date
FR2288312B1 (en) 1977-03-18
NL7512025A (en) 1976-04-20
DE2545980A1 (en) 1976-05-06
FR2288312A1 (en) 1976-05-14
GB1531974A (en) 1978-11-15
CA1071535A (en) 1980-02-12
JPS5191325A (en) 1976-08-10
BE834248A (en) 1976-04-06

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