ES372113A1 - Prothrombin complex - Google Patents

Prothrombin complex

Info

Publication number
ES372113A1
ES372113A1 ES372113A ES372113A ES372113A1 ES 372113 A1 ES372113 A1 ES 372113A1 ES 372113 A ES372113 A ES 372113A ES 372113 A ES372113 A ES 372113A ES 372113 A1 ES372113 A1 ES 372113A1
Authority
ES
Spain
Prior art keywords
supernatant
calcium phosphate
precipitate
tribasic calcium
prothrombin complex
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES372113A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxter International Inc
Original Assignee
Baxter Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Laboratories Inc filed Critical Baxter Laboratories Inc
Publication of ES372113A1 publication Critical patent/ES372113A1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6429—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21005—Thrombin (3.4.21.5)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

A method of preparing a prothrombin complex comprises adjusting the pH of an aqueous suspension of Cohn Plasma Fraction III to within a range of from about 7.0 to about 7.4, separating the resulting suspension and thoroughly mixing the supernatant with tribasic calcium phosphate to adsorb the coagulation factors, thoroughly mixing the tribasic calcium phosphate adsorbed - protein precipitate with trisodium citrate to elute the coagulation factors, separating the resulting suspension and subjecting the supernatant to a succession of two cold ethanol precipitations, first at a pH of about 6.8 and a final concentration of from about 14% to about 16% ethanol at about -5 C with retention of the supernatant, and then at a pH of about 5.2 and a final concentration of about 25% ethanol at about -7 C with retention of the precipitate as the active prothrombin complex. After removal of the tribasic calcium phosphate precipitate, an anticoagulant such as heparin may be added to the solution.
ES372113A 1968-10-02 1969-10-02 Prothrombin complex Expired ES372113A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US76465868A 1968-10-02 1968-10-02

Publications (1)

Publication Number Publication Date
ES372113A1 true ES372113A1 (en) 1971-09-16

Family

ID=25071363

Family Applications (1)

Application Number Title Priority Date Filing Date
ES372113A Expired ES372113A1 (en) 1968-10-02 1969-10-02 Prothrombin complex

Country Status (9)

Country Link
BE (1) BE738269A (en)
CA (1) CA920947A (en)
DE (1) DE1949314A1 (en)
DK (1) DK122105B (en)
ES (1) ES372113A1 (en)
FR (1) FR2035810B1 (en)
GB (1) GB1222810A (en)
IL (1) IL32859A0 (en)
NL (1) NL6914928A (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4304700A (en) 1979-08-27 1981-12-08 Celanese Corporation Two component aqueous based coating composition
JPH0686385B2 (en) * 1980-01-28 1994-11-02 バクスタ−、インタ−ナショナル、インコ−ポレイテッド Active prothrombin complex composition and method for producing the same
US5659017A (en) * 1995-11-07 1997-08-19 Alpha Therapeutic Corporation Anion exchange process for the purification of Factor VIII
CA3078625C (en) 2017-10-09 2023-01-17 Terumo Bct Biotechnologies, Llc Lyophilization container and method of using same
WO2020242552A1 (en) 2019-03-14 2020-12-03 Terumo Bct Biotechnologies, Llc Multi-part lyophilization container and method of use

Also Published As

Publication number Publication date
IL32859A0 (en) 1969-11-12
FR2035810A1 (en) 1970-12-24
BE738269A (en) 1970-02-16
FR2035810B1 (en) 1973-01-12
NL6914928A (en) 1970-04-06
DE1949314A1 (en) 1970-04-16
DK122105B (en) 1972-01-24
GB1222810A (en) 1971-02-17
CA920947A (en) 1973-02-13

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