ES2797480T3 - Un procedimiento de cromatografía de reparto débil - Google Patents

Un procedimiento de cromatografía de reparto débil Download PDF

Info

Publication number
ES2797480T3
ES2797480T3 ES06738028T ES06738028T ES2797480T3 ES 2797480 T3 ES2797480 T3 ES 2797480T3 ES 06738028 T ES06738028 T ES 06738028T ES 06738028 T ES06738028 T ES 06738028T ES 2797480 T3 ES2797480 T3 ES 2797480T3
Authority
ES
Spain
Prior art keywords
product
medium
protein
column
conditions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES06738028T
Other languages
English (en)
Spanish (es)
Inventor
Paul Brown
Jon Coffman
Ranganathan Godavarti
Tim Iskra
Brian D Kelley
Suresh Vunnum
Shujun Sun
Tianning Yu
James Booth
Mary Beth Switzer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wyeth LLC
Original Assignee
Wyeth LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36992346&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2797480(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Wyeth LLC filed Critical Wyeth LLC
Application granted granted Critical
Publication of ES2797480T3 publication Critical patent/ES2797480T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/30—Partition chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
ES06738028T 2005-03-11 2006-03-10 Un procedimiento de cromatografía de reparto débil Expired - Lifetime ES2797480T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US66043705P 2005-03-11 2005-03-11
PCT/US2006/008919 WO2006099308A2 (en) 2005-03-11 2006-03-10 A method of weak partitioning chromatography

Publications (1)

Publication Number Publication Date
ES2797480T3 true ES2797480T3 (es) 2020-12-02

Family

ID=36992346

Family Applications (1)

Application Number Title Priority Date Filing Date
ES06738028T Expired - Lifetime ES2797480T3 (es) 2005-03-11 2006-03-10 Un procedimiento de cromatografía de reparto débil

Country Status (21)

Country Link
US (3) US8067182B2 (https=)
EP (1) EP1869065B1 (https=)
JP (1) JP5199063B2 (https=)
KR (2) KR101660575B1 (https=)
CN (2) CN104610422A (https=)
AU (1) AU2006223180B2 (https=)
BR (1) BRPI0608584B8 (https=)
CA (1) CA2601062C (https=)
CR (1) CR9377A (https=)
DK (1) DK1869065T3 (https=)
ES (1) ES2797480T3 (https=)
HK (1) HK1210476A1 (https=)
HU (1) HUE049797T2 (https=)
IL (1) IL185687A (https=)
MX (1) MX2007011129A (https=)
NO (1) NO20074443L (https=)
PL (1) PL1869065T3 (https=)
PT (1) PT1869065T (https=)
RU (1) RU2457214C2 (https=)
SI (1) SI1869065T1 (https=)
WO (1) WO2006099308A2 (https=)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006223180B2 (en) 2005-03-11 2011-11-24 Wyeth Llc A method of weak partitioning chromatography
US20100234577A1 (en) * 2006-06-14 2010-09-16 Smithkline Beecham Corporation Methods for purifying antibodies using ceramic hydroxyapatite
DK2061803T4 (da) 2006-08-28 2022-12-05 Ares Trading Sa Fremgangsmåde til oprensning af fc-holdige proteiner
WO2008031020A2 (en) * 2006-09-08 2008-03-13 Wyeth Arginine wash in protein purification using affinity chromatography
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
TWI456062B (zh) 2006-09-13 2014-10-11 Abbvie Inc 細胞培養改良
ES2538986T3 (es) * 2006-11-08 2015-06-25 Wyeth Llc Medios diseñados racionalmente para un cultivo celular
CN101889025B (zh) 2007-10-30 2013-11-13 健泰科生物技术公司 通过阳离子交换层析进行的抗体纯化
EP2275431A3 (en) 2008-01-18 2011-05-25 Bio-Rad Laboratories, Inc. Enhanced fractionation of phosphorylated and nonphosphorylated biomolecules by apatite chromotography
MX2011001506A (es) † 2008-08-14 2011-03-15 Genentech Inc Metodos para eliminar un contaminante utilizado cromatografia de membrana de intercambio de ion en desplazamiento de proteina indigena.
US20110166332A1 (en) * 2008-09-12 2011-07-07 Ge Healthcare Bio-Sciences Ab Enhanced antibody aggregate removal with capto adhere in the presence of protein-excluded zwitterions
US20100069617A1 (en) * 2008-09-12 2010-03-18 Ge Healthcare Bio-Sciences Ab Enhanced protein aggregate removal by mixed mode chromatography on hydrophobic interaction media in the presence of protein-excluded zwitterions
MX2011004201A (es) 2008-10-20 2011-05-24 Abbott Lab Desactivacion viral durante purificacion de anticuerpos.
AU2009347206C1 (en) 2008-10-20 2016-12-08 Abbvie Inc. Isolation and purification of antibodies using Protein A affinity chromatography
EP2362767B1 (en) 2008-10-29 2017-12-06 Ablynx N.V. Formulations of single domain antigen binding molecules
BRPI0919879A2 (pt) 2008-10-29 2016-02-16 Wyeth Llc métodos para purificação de moléculas de ligação a antígeno de domínio único
ES2488542T3 (es) * 2008-12-22 2014-08-27 F. Hoffmann-La Roche Ag Purificación de inmunoglobulinas
EP2513134B1 (en) * 2009-12-18 2017-09-06 Novartis AG Wash solution and method for affinity chromatography
WO2011088225A1 (en) 2010-01-15 2011-07-21 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
EP4492053A3 (en) 2010-05-25 2025-03-19 F. Hoffmann-La Roche AG Methods of purifying polypeptides
EP3266793A1 (en) * 2010-06-21 2018-01-10 Kyowa Hakko Kirin Co., Ltd. Method for purifying protein using amino acid
GB201012603D0 (en) * 2010-07-27 2010-09-08 Ucb Pharma Sa Protein purification
CA2808116C (en) 2010-07-30 2016-06-07 Pfizer Inc. Tandem purification of proteins
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
WO2012068134A1 (en) 2010-11-15 2012-05-24 Biogen Idec Inc. Enrichment and concentration of select product isoforms by overloaded bind and elute chromatography
JP5881744B2 (ja) 2011-02-02 2016-03-09 バイオ−ラッド ラボラトリーズ インコーポレーティッド アルカリ溶液でのアパタイトの表面中和方法
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
CN103827134A (zh) 2011-07-20 2014-05-28 泽普泰恩股份有限公司 多肽分离方法
BR112014010406A2 (pt) 2011-11-02 2017-04-25 Genentech Inc cromatografia de sobrecarga e eluto
SG10201701224UA (en) 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9150645B2 (en) 2012-04-20 2015-10-06 Abbvie, Inc. Cell culture methods to reduce acidic species
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9249182B2 (en) * 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
US9815695B2 (en) * 2012-05-30 2017-11-14 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins
CN104507955A (zh) * 2012-05-31 2015-04-08 新加坡科技研究局 采用具有多形式官能性的颗粒对免疫球蛋白g制备物的色谱纯化
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
HK1211981A1 (en) 2012-09-02 2016-06-03 Abbvie Inc. Methods to control protein heterogeneity
JP6348507B2 (ja) 2012-12-14 2018-06-27 ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ 充填ベッドクロマトグラフィーカラムの洗浄方法
SG11201507230PA (en) 2013-03-12 2015-10-29 Abbvie Inc Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
EP3395423B1 (en) 2013-03-14 2023-09-20 Amgen Inc. Removal of leaked affinity purification ligand
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
WO2014145744A1 (en) 2013-03-15 2014-09-18 Alder Biopharmaceuticals, Inc. Antibody purification and purity monitoring
AR096713A1 (es) 2013-06-25 2016-01-27 Cadila Healthcare Ltd Proceso de purificación para anticuerpos monoclonales
ES2915378T3 (es) 2013-09-13 2022-06-22 Hoffmann La Roche Procedimientos para detectar y cuantificar una proteína de célula huésped en líneas celulares
EP4331605A3 (en) 2013-09-13 2024-05-22 F. Hoffmann-La Roche AG Methods and compositions comprising purified recombinant polypeptides
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
WO2015088677A1 (en) 2013-12-12 2015-06-18 Emd Millipore Corporation Protein separations using an acrylamide containing filter
CN105392569B (zh) 2014-06-23 2019-08-20 生物辐射实验室股份有限公司 磷灰石预处理
US10099157B2 (en) 2014-06-23 2018-10-16 Bio-Rad Laboratories, Inc. Apatite in-situ restoration
US10688412B2 (en) 2016-07-25 2020-06-23 Cehpalon, Inc. Affinity chromatography wash buffer
CN111902720B (zh) 2018-03-21 2025-02-07 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
WO2019224660A1 (ja) * 2018-05-24 2019-11-28 ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 吸着剤の生産方法
EP3826743A4 (en) * 2018-07-25 2022-09-14 Merck Sharp & Dohme Corp. METHODS FOR SEPARATION OF HOST CELL LIPASES FROM A PRODUCTION PROTEIN IN CHROMATOGRAPHIC METHODS
WO2020172658A1 (en) 2019-02-24 2020-08-27 Bristol-Myers Squibb Company Methods of isolating a protein
WO2020191369A1 (en) 2019-03-21 2020-09-24 Codiak Biosciences, Inc. Process for preparing extracellular vesicles
EP4096802A4 (en) * 2020-01-29 2024-07-03 Merck Sharp & Dohme LLC METHOD FOR SEPARATION OF HOST CELL LIPASES FROM ANTI-LAG3 ANTIBODY PRODUCTION
US20240241020A1 (en) 2020-09-23 2024-07-18 Lonza Sales Ag Process for preparing extracellular vesicles
US20230366794A1 (en) 2020-09-23 2023-11-16 Codiak Biosciences, Inc. Process for preparing extracellular vesicles
JP2024532238A (ja) 2021-08-23 2024-09-05 ジェネンテック， インコーポレイテッド 抗体薬物複合体のフロースルー陽イオン交換クロマトグラフィー精製方法
AU2021471146A1 (en) * 2021-10-27 2024-06-13 Plasma Technologies, Llc Compositions and methods for isolating proteins
JP7820943B2 (ja) * 2021-10-28 2026-02-26 花王株式会社 Ｖｈｈ抗体の製造方法
US20260109751A1 (en) * 2022-12-30 2026-04-23 Eli Lilly And Company Methods of dulaglutide purification using hydrophobic interaction chromatography

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4029583A (en) * 1975-02-28 1977-06-14 Purdue Research Foundation Chromatographic supports and methods and apparatus for preparing the same
JPS63126826A (ja) * 1986-11-14 1988-05-30 Asahi Chem Ind Co Ltd プラスミノ−ゲン活性化因子誘導物質
EP0313343B2 (en) * 1987-10-23 2003-07-23 Schering Corporation Method of purifying protein
US5451662A (en) * 1987-10-23 1995-09-19 Schering Corporation Method of purifying protein
US4983722A (en) * 1988-06-08 1991-01-08 Miles Inc. Removal of protein A from antibody preparations
DE68919647T2 (de) 1988-10-26 1995-05-11 American Cyanamid Co Verfahren zur Verringerung des Aggregatinhaltes in Wachstumshormonen gleichenden Stoffen.
GB9022547D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Purified immunoglobulin
US5264555A (en) 1992-07-14 1993-11-23 Enzon, Inc. Process for hemoglobin extraction and purification
FR2703049B1 (fr) * 1993-03-22 1995-04-21 Rhone Poulenc Rorer Sa Procédé de purification des taxoïdes.
CA2106612C (en) 1993-09-21 2001-02-06 Diana Pliura Displacement chromatography process
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
AU7241294A (en) * 1994-04-01 1995-10-23 Unisyn Technologies, Inc. High salt binding buffer for immunoglobulin purification with a synthetic affinity ligand
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
RU2094077C1 (ru) * 1996-07-23 1997-10-27 Акционерное общество закрытого типа "Фосфосорб" Хроматографический способ выделения альфа-фетопротеина
TW505655B (en) * 1997-10-14 2002-10-11 Tanox Inc Enhanced aggregate removal from bulk-biologicals using ion exchange chromatography
US6504013B1 (en) 2000-02-01 2003-01-07 Idexx Laboratories, Inc. Canine allergy therapeutic recombinant chimeric anti-IgE monoclonal antibody
WO2000015260A1 (en) 1998-09-16 2000-03-23 Tanox, Inc. Anti-ige gene therapy
ATE266672T1 (de) * 1998-12-30 2004-05-15 Folke Tjerneld Trennungsmethode unter verwendung von flüssig- flüssig partition
WO2003059935A2 (en) 2001-12-21 2003-07-24 Immunex Corporation Methods for purifying protein
GB0304576D0 (en) * 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
AU2004215653B2 (en) 2003-02-28 2011-03-17 Lonza Biologics Plc. Antibody purification by protein A and ion exchange chromatography
WO2005010528A1 (ja) * 2003-07-28 2005-02-03 Reverse Proteomics Research Institute Co., Ltd. 非特異的物質の除去方法
DE602004026343D1 (de) 2003-10-24 2010-05-12 Amgen Inc Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen
EP2336172B1 (en) 2003-10-27 2014-12-10 Wyeth LLC Removal of high molecular weight aggregates using hydroxyapatite chromatography
AU2006223180B2 (en) 2005-03-11 2011-11-24 Wyeth Llc A method of weak partitioning chromatography

Also Published As

Publication number Publication date
HK1210476A1 (en) 2016-04-22
SI1869065T1 (sl) 2020-08-31
WO2006099308A2 (en) 2006-09-21
KR20070121667A (ko) 2007-12-27
MX2007011129A (es) 2007-11-06
PT1869065T (pt) 2020-06-18
DK1869065T3 (da) 2020-06-08
BRPI0608584B1 (pt) 2018-02-06
NO20074443L (no) 2007-09-27
US20070054390A1 (en) 2007-03-08
EP1869065A2 (en) 2007-12-26
KR20130128020A (ko) 2013-11-25
US20070060741A1 (en) 2007-03-15
US9144755B2 (en) 2015-09-29
EP1869065B1 (en) 2020-05-06
AU2006223180A1 (en) 2006-09-21
JP5199063B2 (ja) 2013-05-15
IL185687A0 (en) 2008-01-06
HUE049797T2 (hu) 2020-10-28
RU2457214C2 (ru) 2012-07-27
US20120138537A1 (en) 2012-06-07
WO2006099308A3 (en) 2007-10-25
CR9377A (es) 2008-02-21
CA2601062A1 (en) 2006-09-21
KR101660575B1 (ko) 2016-09-27
JP2008533473A (ja) 2008-08-21
IL185687A (en) 2015-05-31
CN104610422A (zh) 2015-05-13
CA2601062C (en) 2016-08-02
BRPI0608584A2 (pt) 2010-01-19
KR101482791B1 (ko) 2015-01-21
PL1869065T3 (pl) 2020-09-21
CN101175765A (zh) 2008-05-07
RU2007132851A (ru) 2009-04-20
BRPI0608584B8 (pt) 2021-05-25
AU2006223180B2 (en) 2011-11-24
US8067182B2 (en) 2011-11-29
EP1869065A4 (en) 2012-07-04

Similar Documents

Publication Publication Date Title
ES2797480T3 (es) Un procedimiento de cromatografía de reparto débil
US9957318B2 (en) Protein purification methods to reduce acidic species
US7385040B2 (en) Antibody purification
US20160272674A1 (en) Isolation and purification of antibodies
US20160115193A1 (en) Novel purification of antibodies using hydrophobic interaction chromatography
BR122020007658B1 (pt) Método para a remoção de agregados de elevado peso molecular utilizando cromatografia de hidroxiapatita
US20080058507A1 (en) Method For The Removal Of Aggregate Proteins From Recombinant Samples Using Ion Exchange Chromatography
Dong et al. A two-column process for bispecific antibody purification based on MabSelect VL resin’s strong byproduct removal capability
US20230406881A1 (en) Buffers and methods for purifying proteins