ES2655069T3 - Nanoparticles comprising RNA ligands - Google Patents

Nanoparticles comprising RNA ligands Download PDF

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Publication number
ES2655069T3
ES2655069T3 ES10181903.5T ES10181903T ES2655069T3 ES 2655069 T3 ES2655069 T3 ES 2655069T3 ES 10181903 T ES10181903 T ES 10181903T ES 2655069 T3 ES2655069 T3 ES 2655069T3
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ES
Spain
Prior art keywords
nanoparticles
rna
core
mrna
nanoparticle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES10181903.5T
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Spanish (es)
Inventor
Thomas William Rademacher
Khalid Gumaa
Manuel Martin-Lomas
Soledad Penades
Rafael Ojeda
Africa Barrientos
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Midatech Ltd
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Midatech Ltd
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Publication of ES2655069T3 publication Critical patent/ES2655069T3/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0063—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
    • A61K49/18—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes
    • A61K49/1818—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/12—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by a special physical form, e.g. emulsion, microcapsules, liposomes, characterized by a special physical form, e.g. emulsions, dispersions, microcapsules
    • A61K51/1241—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by a special physical form, e.g. emulsion, microcapsules, liposomes, characterized by a special physical form, e.g. emulsions, dispersions, microcapsules particles, powders, lyophilizates, adsorbates, e.g. polymers or resins for adsorption or ion-exchange resins
    • A61K51/1255—Granulates, agglomerates, microspheres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function

Abstract

Un procedimiento de detección y/u obtención de imágenes de ARNm empleando nanopartículas que comprenden un núcleo que incluye átomos metálicos y/o semiconductores, en donde el núcleo está ligado covalentemente a una pluralidad de ligandos que comprenden: (i) al menos un ligando de ARN que comprende (a) una molécula de ARNip o de miARN de entre 17 y 30 ribonucleótidos de longitud; y (ii) un ligando que comprende un grupo de hidrato de carbono, en donde el procedimiento comprende poner en contacto las nanopartículas con una muestra o células que contienen ARNm diana en condiciones en las que los ligandos de ARN presentes en las nanopartículas sean capaces de interaccionar con ARNm diana y detectar el complejo nanopartícula-ARN-ARNm, y en donde el núcleo de la nanopartícula tiene un diámetro medio entre 0,5 y 10 nm.A method of detecting and / or obtaining mRNA images using nanoparticles comprising a core that includes metal atoms and / or semiconductors, wherein the core is covalently linked to a plurality of ligands comprising: (i) at least one ligand of RNA comprising (a) an siRNA or miRNA molecule between 17 and 30 ribonucleotides in length; and (ii) a ligand comprising a carbohydrate group, wherein the method comprises contacting the nanoparticles with a sample or cells containing target mRNA under conditions where the RNA ligands present in the nanoparticles are capable of interact with target mRNA and detect the nanoparticle-RNA-mRNA complex, and where the core of the nanoparticle has an average diameter between 0.5 and 10 nm.

Description

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Claims (1)

imagen1image 1
ES10181903.5T 2004-05-24 2005-05-24 Nanoparticles comprising RNA ligands Active ES2655069T3 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US57380504P 2004-05-24 2004-05-24
GB0411537 2004-05-24
GBGB0411537.4A GB0411537D0 (en) 2004-05-24 2004-05-24 Nanoparticles comprising rna ligands
US573805P 2004-05-24

Publications (1)

Publication Number Publication Date
ES2655069T3 true ES2655069T3 (en) 2018-02-16

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
ES10181903.5T Active ES2655069T3 (en) 2004-05-24 2005-05-24 Nanoparticles comprising RNA ligands
ES05746540.3T Active ES2533238T3 (en) 2004-05-24 2005-05-24 Nanoparticles comprising RNA ligands

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES05746540.3T Active ES2533238T3 (en) 2004-05-24 2005-05-24 Nanoparticles comprising RNA ligands

Country Status (3)

Country Link
CN (2) CN102872463B (en)
ES (2) ES2655069T3 (en)
GB (1) GB0411537D0 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008073856A2 (en) * 2006-12-08 2008-06-19 Massachusetts Institute Of Technology Delivery of nanoparticles and/or agents to cells
CA2744207C (en) 2008-11-24 2019-05-28 Northwestern University Polyvalent rna-nanoparticle compositions
US20100233270A1 (en) 2009-01-08 2010-09-16 Northwestern University Delivery of Oligonucleotide-Functionalized Nanoparticles
KR20120022938A (en) * 2009-04-15 2012-03-12 노오쓰웨스턴 유니버시티 Delivery of oligonucleotide-functionalized nanoparticles
CN102631687A (en) * 2012-05-07 2012-08-15 西安电子科技大学 Multifunctional magnetic nano-carrier for targeted delivery of microRNA, preparation method and application thereof
KR20150020288A (en) * 2012-06-08 2015-02-25 에트리스 게엠베하 Pulmonary delivery of messenger rna
US20150148529A1 (en) * 2012-06-20 2015-05-28 Japan Science And Technology Agency Nucleic acid complex and nucleic acid-polysaccharide complex
US10894963B2 (en) 2013-07-25 2021-01-19 Exicure, Inc. Spherical nucleic acid-based constructs as immunostimulatory agents for prophylactic and therapeutic use
WO2015126502A2 (en) 2013-12-03 2015-08-27 Northwestern University Liposomal particles, methods of making same and uses thereof
CN107106493A (en) 2014-11-21 2017-08-29 西北大学 The sequence-specific cellular uptake of spherical nucleic acid nano particle conjugate
CN111110846B (en) * 2018-10-30 2021-07-23 国家纳米科学中心 Metal-nucleic acid nano-particle and preparation method and application thereof
CN113413467A (en) * 2021-07-01 2021-09-21 清华大学 Carrier-free mRNA delivery method

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69737598T2 (en) * 1996-07-29 2007-12-27 Nanosphere Inc., Skokie NANOPARTICLES WITH OLIGONUCLEOTIDES ADDITED TO IT AND THEIR USES
GB0025414D0 (en) * 2000-10-16 2000-11-29 Consejo Superior Investigacion Nanoparticles

Also Published As

Publication number Publication date
CN102872463B (en) 2015-04-08
CN102872463A (en) 2013-01-16
CN101180400B (en) 2013-07-10
ES2533238T3 (en) 2015-04-08
CN101180400A (en) 2008-05-14
GB0411537D0 (en) 2004-06-23

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