ES2610633T3 - Método para generar aptámeros con velocidades de disociación mejoradas - Google Patents
Método para generar aptámeros con velocidades de disociación mejoradas Download PDFInfo
- Publication number
- ES2610633T3 ES2610633T3 ES10797920.5T ES10797920T ES2610633T3 ES 2610633 T3 ES2610633 T3 ES 2610633T3 ES 10797920 T ES10797920 T ES 10797920T ES 2610633 T3 ES2610633 T3 ES 2610633T3
- Authority
- ES
- Spain
- Prior art keywords
- aptamer
- target
- aptamers
- tissue
- dissociation rate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 0 *c(cc1[N+]([O-])=O)ccc1NCCCCCC(NCCOCCOC(NCCCCCCOP([O-])(O*)=O)=O)=O Chemical compound *c(cc1[N+]([O-])=O)ccc1NCCCCCC(NCCOCCOC(NCCCCCCOP([O-])(O*)=O)=O)=O 0.000 description 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1048—SELEX
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US499967 | 2000-02-08 | ||
| US12/499,967 US7964356B2 (en) | 2007-01-16 | 2009-07-09 | Method for generating aptamers with improved off-rates |
| PCT/US2010/041540 WO2011006075A1 (en) | 2009-07-09 | 2010-07-09 | Method for generating aptamers with improved off-rates |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2610633T3 true ES2610633T3 (es) | 2017-04-28 |
Family
ID=43429566
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES10797920.5T Active ES2610633T3 (es) | 2009-07-09 | 2010-07-09 | Método para generar aptámeros con velocidades de disociación mejoradas |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7964356B2 (https=) |
| EP (1) | EP2451981B1 (https=) |
| JP (2) | JP5926178B2 (https=) |
| AU (1) | AU2010271271B2 (https=) |
| CA (2) | CA2982304A1 (https=) |
| ES (1) | ES2610633T3 (https=) |
| WO (1) | WO2011006075A1 (https=) |
Families Citing this family (78)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070166741A1 (en) | 1998-12-14 | 2007-07-19 | Somalogic, Incorporated | Multiplexed analyses of test samples |
| US7947447B2 (en) | 2007-01-16 | 2011-05-24 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| US20110136099A1 (en) * | 2007-01-16 | 2011-06-09 | Somalogic, Inc. | Multiplexed Analyses of Test Samples |
| US7855054B2 (en) * | 2007-01-16 | 2010-12-21 | Somalogic, Inc. | Multiplexed analyses of test samples |
| US7964356B2 (en) | 2007-01-16 | 2011-06-21 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| US8975026B2 (en) * | 2007-01-16 | 2015-03-10 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| US8586303B1 (en) | 2007-01-22 | 2013-11-19 | Steven Albert Benner | In vitro selection with expanded genetic alphabets |
| US9725713B1 (en) | 2007-01-22 | 2017-08-08 | Steven A Benner | In vitro selection with expanded genetic alphabets |
| US20100151465A1 (en) | 2008-03-27 | 2010-06-17 | Jingyue Ju | Selective Capture and Release of Analytes |
| US8404830B2 (en) * | 2007-07-17 | 2013-03-26 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| US8906700B2 (en) | 2007-11-06 | 2014-12-09 | Ambergen, Inc. | Methods and compositions for phototransfer |
| US8703416B2 (en) | 2008-07-17 | 2014-04-22 | Somalogic, Inc. | Method for purification and identification of sperm cells |
| WO2012006632A2 (en) | 2010-07-09 | 2012-01-12 | Somalogic, Inc. | Lung cancer biomarkers and uses thereof |
| US9090663B2 (en) * | 2009-04-21 | 2015-07-28 | The Trustees Of Columbia University In The City Of New York | Systems and methods for the capture and separation of microparticles |
| US8841429B2 (en) * | 2009-11-03 | 2014-09-23 | Vivonics, Inc. | Nucleic acid ligands against infectious prions |
| US8236570B2 (en) | 2009-11-03 | 2012-08-07 | Infoscitex | Methods for identifying nucleic acid ligands |
| WO2012055573A1 (en) * | 2010-10-29 | 2012-05-03 | Noxxon Pharma Ag | Use of hepcidin binding nucleic acids for depletion of hepcidin from the body |
| AU2012225273B2 (en) * | 2011-03-10 | 2016-10-13 | Somalogic, Inc. | Aptamers for Clostridium difficile diagnostics |
| WO2012174496A2 (en) * | 2011-06-17 | 2012-12-20 | Somalogic, Inc. | Method for purification and identification of sperm cells |
| WO2013019714A1 (en) | 2011-07-29 | 2013-02-07 | The Trustees Of Columbia University In The City Of New York | Mems affinity sensor for continuous monitoring of analytes |
| WO2013067268A1 (en) * | 2011-11-03 | 2013-05-10 | Tripath Imaging, Inc. | Methods and compositions for preparing samples for immunostaining |
| EP2798085B1 (en) | 2011-12-30 | 2018-01-24 | Quest Diagnostics Investments Incorporated | Aptamers and diagnostic methods for detecting the egf receptor |
| TWI465566B (zh) * | 2012-06-07 | 2014-12-21 | Univ Nat Taiwan | 具專一性核適體之製備方法及套組 |
| WO2013185078A1 (en) | 2012-06-07 | 2013-12-12 | Somalogic, Inc. | Aptamer-based multiplexed assays |
| WO2014068553A1 (en) * | 2012-10-29 | 2014-05-08 | Yeda Research And Development Co. Ltd. | Aptamers, multimeric aptamers and uses thereof |
| CA2902186C (en) * | 2013-03-14 | 2023-04-11 | Somalogic, Inc. | Aptamers that bind to il-6 and their use in treating or diagnosing il-6 mediated conditions |
| US9695424B2 (en) | 2013-09-09 | 2017-07-04 | Somalogic, Inc. | PDGF and VEGF aptamers having improved stability and their use in treating PDGF and VEGF mediated diseases and disorders |
| WO2016022696A1 (en) | 2014-08-05 | 2016-02-11 | The Trustees Of Columbia University In The City Of New York | Method of isolating aptamers for minimal residual disease detection |
| WO2016123058A1 (en) | 2015-01-27 | 2016-08-04 | Somalogic, Inc. | Biomarkers for detection of tuberculosis risk |
| US10577609B2 (en) * | 2016-03-07 | 2020-03-03 | Postech Academy—Industry Foundation | Glypican-3 specific aptamer and use thereof |
| EP4339288A3 (en) * | 2016-03-18 | 2024-06-05 | Caris Science, Inc. | Oligonucleotide probes and uses thereof |
| ES2972849T3 (es) | 2016-10-14 | 2024-06-17 | Cns Biosciences Inc | Tratamiento del dolor neuropático en personas con lesión de la médula espinal |
| DK3548652T3 (da) | 2016-12-01 | 2024-07-08 | Nautilus Subsidiary Inc | Fremgangsmåder til proteinanalyse |
| KR20250152680A (ko) | 2017-12-29 | 2025-10-23 | 노틸러스 서브시디어리, 인크. | 단백질 식별을 위한 디코딩 접근법 |
| US10481155B2 (en) | 2018-03-09 | 2019-11-19 | Somalogic, Inc. | Proteomic assay using quantum sensors |
| WO2019202448A1 (en) | 2018-04-16 | 2019-10-24 | University Of Cape Town | A three-protein proteomic biomarker for prospective determination of risk for development of active tuberculosis |
| EP3810563A4 (en) | 2018-06-22 | 2022-03-23 | Somalogic, Inc. | IMPROVED PROTEOMIC MULTIPLEX TESTS |
| WO2020117914A1 (en) | 2018-12-04 | 2020-06-11 | Roche Sequencing Solutions, Inc. | Spatially oriented quantum barcoding of cellular targets |
| BR112021010715A2 (pt) | 2018-12-20 | 2021-11-16 | Ksq Therapeutics Inc | Pirazolopirimidinas substituídas e purinas substituídas e seu uso como inibidores de protease de processamento ubiquitina-específica 1 (usp1) |
| WO2020204151A1 (ja) * | 2019-04-04 | 2020-10-08 | 株式会社リボミック | Ｆｇｆ９に対するアプタマー及びその使用 |
| JP7323162B2 (ja) * | 2019-06-27 | 2023-08-08 | 株式会社アドバンジェン | 核酸アプタマー |
| US20230167453A1 (en) * | 2020-04-02 | 2023-06-01 | Board Of Regents, The University Of Texas System | Rna aptamers and use thereof for treating cancer |
| AU2021288692A1 (en) | 2020-06-11 | 2023-02-02 | Nautilus Subsidiary, Inc. | Methods and systems for computational decoding of biological, chemical, and physical entities |
| GB2628478A (en) * | 2020-10-29 | 2024-09-25 | Ambergen Inc | Novel photocleavable mass-tags for multiplexed mass spectrometric imaging of tissues using biomolecular probes |
| KR20230098186A (ko) | 2020-10-30 | 2023-07-03 | 케이에스큐 세러퓨틱스 인코포레이티드 | 치환된 피라졸로피리미딘의 고체 상태 형태 및 이의 용도 |
| EP4281774B1 (en) | 2021-01-20 | 2025-11-26 | Nautilus Subsidiary, Inc. | Biomolecule quantitation |
| EP4281775B1 (en) | 2021-01-21 | 2026-04-29 | Nautilus Subsidiary, Inc. | Systems and methods for biomolecule preparation |
| US12037637B2 (en) * | 2021-01-25 | 2024-07-16 | The Broad Institute, Inc. | Photoselective non-invasive targeted genomic and epigenomic sequencing of spatially-defined cells or subcellular regions |
| IL305336B2 (en) | 2021-03-11 | 2025-09-01 | Nautilus Subsidiary Inc | Systems and methods for preserving biomolecules |
| WO2022232308A1 (en) | 2021-04-27 | 2022-11-03 | Singular Genomics Systems, Inc. | High density sequencing and multiplexed priming |
| CA3227872A1 (en) | 2021-09-09 | 2023-03-16 | James Henry JOLY | Characterization and localization of protein modifications |
| CA3227592A1 (en) | 2021-09-22 | 2023-03-30 | Gregory KAPP | Methods and systems for determining polypeptide interactions |
| WO2023066881A1 (en) | 2021-10-18 | 2023-04-27 | Astrazeneca Ab | Inhibition of map3k15 for treating and preventing diabetes |
| US20230149883A1 (en) | 2021-11-03 | 2023-05-18 | Nautilus Biotechnology, Inc. | Systems and methods for surface structuring |
| US12092578B2 (en) | 2022-03-29 | 2024-09-17 | Nautilus Subsidiary, Inc. | Integrated arrays for single-analyte processes |
| US20240287583A1 (en) | 2022-04-08 | 2024-08-29 | Illumina, Inc. | Aptamer dynamic range compression and detection techniques |
| WO2023212490A1 (en) | 2022-04-25 | 2023-11-02 | Nautilus Subsidiary, Inc. | Systems and methods for assessing and improving the quality of multiplex molecular assays |
| EP4544077A1 (en) | 2022-06-21 | 2025-04-30 | Nautilus Subsidiary, Inc. | Method for detecting analytes at sites of optically non-resolvable distances |
| WO2024059655A1 (en) | 2022-09-15 | 2024-03-21 | Nautilus Subsidiary, Inc. | Characterizing accessibility of macromolecule structures |
| WO2024073599A1 (en) | 2022-09-29 | 2024-04-04 | Nautilus Subsidiary, Inc. | Preparation of array surfaces for single-analyte processes |
| WO2024107857A1 (en) | 2022-11-15 | 2024-05-23 | Nautilus Subsidiary, Inc. | Standard polypeptides |
| WO2024124073A1 (en) | 2022-12-09 | 2024-06-13 | Nautilus Subsidiary, Inc. | A method comprising performing on a single-analyte array at least 50 cycles of a process |
| US20240201182A1 (en) | 2022-12-15 | 2024-06-20 | Nautilus Subsidiary, Inc. | Inhibition of photon phenomena on single molecule arrays |
| US20240241110A1 (en) | 2023-01-12 | 2024-07-18 | Nautilus Subsidiary, Inc. | Characterization of glycans and glycoconjugates |
| CN119452097A (zh) | 2023-03-10 | 2025-02-14 | 因美纳有限公司 | 适体检测技术 |
| EP4689173A1 (en) | 2023-03-24 | 2026-02-11 | Nautilus Subsidiary, Inc. | Improved transfer of nanoparticles to array surfaces |
| WO2024216233A1 (en) | 2023-04-13 | 2024-10-17 | Nautilus Subsidiary, Inc. | Artificial proteins for displaying epitopes |
| US20240394902A1 (en) | 2023-05-24 | 2024-11-28 | Nautilus Subsidiary, Inc. | Systems and methods for machine vision |
| WO2025006625A1 (en) | 2023-06-26 | 2025-01-02 | Illumina, Inc. | Interlocking nucleic acids for target hybridization |
| WO2025010324A1 (en) | 2023-07-06 | 2025-01-09 | Somalogic Operating Co., Inc. | Detection of target molecules in dried biologic matrices |
| WO2025049720A2 (en) | 2023-08-31 | 2025-03-06 | Illumina, Inc. | Aptamer dynamic range compression and detection techniques |
| WO2025049331A2 (en) | 2023-08-31 | 2025-03-06 | Illumina, Inc. | Aptamer detection techniques |
| WO2025064457A1 (en) | 2023-09-19 | 2025-03-27 | Somalogic Operating Co., Inc. | Methods of solid-phase nucleic acid hybridization |
| US20250189519A1 (en) | 2023-12-11 | 2025-06-12 | Nautilus Subsidiary, Inc. | Methods and compositions of particle-based arrays |
| WO2025137166A1 (en) | 2023-12-20 | 2025-06-26 | Nautilus Subsidiary, Inc. | Fluidic devices for single-analyte assays |
| WO2025178951A1 (en) | 2024-02-22 | 2025-08-28 | Illumina, Inc. | Techniques for dynamic range compression grouping in analyte assays |
| US20250298016A1 (en) | 2024-03-22 | 2025-09-25 | Nautilus Subsidiary, Inc. | Plasmonic particle systems for single-analyte assays |
| WO2025212338A1 (en) | 2024-04-01 | 2025-10-09 | Nautilus Subsidiary, Inc. | Nanostructures for modulation of analyte conformation |
Family Cites Families (70)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5599720A (en) | 1982-08-27 | 1997-02-04 | Multilyte Limited | Measurement of analyte concentration |
| GB8314523D0 (en) | 1983-05-25 | 1983-06-29 | Lowe C R | Diagnostic device |
| US4737453A (en) | 1984-12-12 | 1988-04-12 | Immunomedics, Inc. | Sandwich immunoassay utilizing a separation specific binding substance |
| GB2183661B (en) | 1985-03-30 | 1989-06-28 | Marc Ballivet | Method for obtaining dna, rna, peptides, polypeptides or proteins by means of a dna recombinant technique |
| US4752566A (en) | 1985-12-17 | 1988-06-21 | Genetics Institute, Inc. | Displacement polynucleotide method and reagent complex employing labeled probe polynucleotide |
| US5124246A (en) | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| WO1989006694A1 (en) | 1988-01-15 | 1989-07-27 | Trustees Of The University Of Pennsylvania | Process for selection of proteinaceous substances which mimic growth-inducing molecules |
| US5763177A (en) * | 1990-06-11 | 1998-06-09 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex |
| US5861254A (en) * | 1997-01-31 | 1999-01-19 | Nexstar Pharmaceuticals, Inc. | Flow cell SELEX |
| US5567588A (en) | 1990-06-11 | 1996-10-22 | University Research Corporation | Systematic evolution of ligands by exponential enrichment: Solution SELEX |
| US6127119A (en) | 1990-06-11 | 2000-10-03 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands of tissue target |
| US5660985A (en) * | 1990-06-11 | 1997-08-26 | Nexstar Pharmaceuticals, Inc. | High affinity nucleic acid ligands containing modified nucleotides |
| US5750342A (en) | 1990-06-11 | 1998-05-12 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands of tissue target |
| US5864026A (en) | 1990-06-11 | 1999-01-26 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: tissue selex |
| US5874218A (en) | 1990-06-11 | 1999-02-23 | Nexstar Pharmaceuticals, Inc. | Method for detecting a target compound in a substance using a nucleic acid ligand |
| US5580737A (en) | 1990-06-11 | 1996-12-03 | Nexstar Pharmaceuticals, Inc. | High-affinity nucleic acid ligands that discriminate between theophylline and caffeine |
| US5459015A (en) | 1990-06-11 | 1995-10-17 | Nexstar Pharmaceuticals, Inc. | High-affinity RNA ligands of basic fibroblast growth factor |
| US5763566A (en) | 1990-06-11 | 1998-06-09 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: tissue SELEX |
| US5789157A (en) | 1990-06-11 | 1998-08-04 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: tissue selex |
| US5688935A (en) | 1990-06-11 | 1997-11-18 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands of tissue target |
| US5712375A (en) | 1990-06-11 | 1998-01-27 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: tissue selex |
| US6716580B2 (en) | 1990-06-11 | 2004-04-06 | Somalogic, Inc. | Method for the automated generation of nucleic acid ligands |
| US5496938A (en) | 1990-06-11 | 1996-03-05 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands to HIV-RT and HIV-1 rev |
| US6001577A (en) | 1998-06-08 | 1999-12-14 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex |
| US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
| US5707796A (en) | 1990-06-11 | 1998-01-13 | Nexstar Pharmaceuticals, Inc. | Method for selecting nucleic acids on the basis of structure |
| US5705337A (en) | 1990-06-11 | 1998-01-06 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: chemi-SELEX |
| US5962219A (en) | 1990-06-11 | 1999-10-05 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: chemi-selex |
| EP0786469B1 (en) | 1990-06-11 | 2006-03-01 | Gilead Sciences, Inc. | Methods of use of nucleic acid ligands |
| AU1456092A (en) | 1991-02-21 | 1992-09-15 | Gilead Sciences, Inc. | Aptamers specific for thrombin and methods of use |
| CA2104698A1 (en) | 1991-02-21 | 1992-08-22 | John J. Toole | Aptamers specific for biomolecules and methods of making |
| US5582981A (en) | 1991-08-14 | 1996-12-10 | Gilead Sciences, Inc. | Method for identifying an oligonucleotide aptamer specific for a target |
| US5412087A (en) | 1992-04-24 | 1995-05-02 | Affymax Technologies N.V. | Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces |
| AU4842693A (en) | 1992-09-11 | 1994-04-12 | Duke University | Method for deriving epitopes |
| US5580972A (en) | 1993-06-14 | 1996-12-03 | Nexstar Pharmaceuticals, Inc. | Purine nucleoside modifications by palladium catalyzed methods |
| US5428149A (en) | 1993-06-14 | 1995-06-27 | Washington State University Research Foundation | Method for palladium catalyzed carbon-carbon coulping and products |
| US6458539B1 (en) | 1993-09-17 | 2002-10-01 | Somalogic, Inc. | Photoselection of nucleic acid ligands |
| US5614503A (en) | 1993-11-12 | 1997-03-25 | Aronex Pharmaceuticals, Inc. | Amphipathic nucleic acid transporter |
| US5681697A (en) | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| US5658738A (en) * | 1994-05-31 | 1997-08-19 | Becton Dickinson And Company | Bi-directional oligonucleotides that bind thrombin |
| US5681702A (en) | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
| DE69633227T2 (de) | 1995-05-03 | 2005-09-15 | Gilead Sciences, Inc., Foster City | Systematische evoultion von liganden durch exponentielle anreicherung: gewebe-selex |
| US6013443A (en) | 1995-05-03 | 2000-01-11 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: tissue SELEX |
| US5945527A (en) * | 1996-05-30 | 1999-08-31 | Nexstar Pharmaceuticals, Inc. | Palladium catalyzed nucleoside modification methods using nucleophiles and carbon monoxide |
| WO1999027133A1 (en) | 1997-11-26 | 1999-06-03 | Medical Research Council | Improved selex procedure and an anti-cd4 aptamer |
| US20070166741A1 (en) | 1998-12-14 | 2007-07-19 | Somalogic, Incorporated | Multiplexed analyses of test samples |
| US6242246B1 (en) | 1997-12-15 | 2001-06-05 | Somalogic, Inc. | Nucleic acid ligand diagnostic Biochip |
| US6376424B1 (en) | 1998-01-21 | 2002-04-23 | Syngenta Crop Protection, Inc. | Herbicidal composition |
| US6403693B2 (en) * | 1999-06-18 | 2002-06-11 | The Goodyear Tire & Rubber Company | Silica reinforced rubber composition and use in tires |
| US6897015B2 (en) | 2000-03-07 | 2005-05-24 | Bioforce Nanosciences, Inc. | Device and method of use for detection and characterization of pathogens and biological materials |
| US8278034B2 (en) | 2000-06-22 | 2012-10-02 | Nuclea Biotechnologies, Inc. | Methods of making frozen tissue microarrays |
| US6376190B1 (en) | 2000-09-22 | 2002-04-23 | Somalogic, Inc. | Modified SELEX processes without purified protein |
| AU2002224401B2 (en) * | 2000-10-16 | 2007-12-06 | Gilead Sciences, Inc. | Nucleic acid ligands to the prostate specific membrane antigen |
| US20030148335A1 (en) | 2001-10-10 | 2003-08-07 | Li Shen | Detecting targets by unique identifier nucleotide tags |
| AU2003217379A1 (en) | 2002-02-15 | 2003-09-09 | Somalogic, Inc. | Methods and reagents for detecting target binding by nucleic acid ligands |
| US20030228603A1 (en) | 2002-04-05 | 2003-12-11 | Cload Sharon T. | Compositions selective for caffeine or aspartame and methods of using same |
| US7767803B2 (en) | 2002-06-18 | 2010-08-03 | Archemix Corp. | Stabilized aptamers to PSMA and their use as prostate cancer therapeutics |
| CA2407825A1 (en) | 2002-10-11 | 2004-04-11 | Andrew J. Simmonds | Trap-tagging: a novel method for the identification and purification of rna-protein complexes |
| US20040176282A1 (en) | 2003-01-09 | 2004-09-09 | Brian Dalby | Cellular delivery and activation of polypeptide-nucleic acid complexes |
| WO2004085049A2 (en) | 2003-03-28 | 2004-10-07 | The Regents Of The University Of California | Preparation and application of encoded bead aggregates in combinatorial chemistry |
| US7672786B2 (en) | 2003-07-02 | 2010-03-02 | Sergey Krylov | Non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM)—based methods for drug and diagnostic development |
| US8062897B2 (en) | 2003-07-21 | 2011-11-22 | Aureon Laboratories, Inc. | Diagnostic histopathology using multiplex gene expression FISH |
| EP1584923A3 (en) | 2004-04-07 | 2006-01-04 | Roche Diagnostics GmbH | Stabilization of biomolecules in samples |
| US7674778B2 (en) | 2004-04-30 | 2010-03-09 | Alnylam Pharmaceuticals | Oligonucleotides comprising a conjugate group linked through a C5-modified pyrimidine |
| WO2007044427A2 (en) | 2005-10-05 | 2007-04-19 | Panomics, Inc. | Detection of nucleic acids from whole blood |
| WO2007084886A2 (en) * | 2006-01-17 | 2007-07-26 | Somalogic, Incorporated | Multiplexed analyses of test samples |
| US7964356B2 (en) | 2007-01-16 | 2011-06-21 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| US7855054B2 (en) | 2007-01-16 | 2010-12-21 | Somalogic, Inc. | Multiplexed analyses of test samples |
| US7947447B2 (en) | 2007-01-16 | 2011-05-24 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| DK2172566T4 (da) | 2007-07-17 | 2022-06-13 | Somalogic Inc | Fremgangsmåde til generering af aptamerer med forbedrede off-rates |
-
2009
- 2009-07-09 US US12/499,967 patent/US7964356B2/en active Active
-
2010
- 2010-07-09 CA CA2982304A patent/CA2982304A1/en not_active Abandoned
- 2010-07-09 WO PCT/US2010/041540 patent/WO2011006075A1/en not_active Ceased
- 2010-07-09 CA CA2765857A patent/CA2765857C/en active Active
- 2010-07-09 ES ES10797920.5T patent/ES2610633T3/es active Active
- 2010-07-09 AU AU2010271271A patent/AU2010271271B2/en active Active
- 2010-07-09 JP JP2012519765A patent/JP5926178B2/ja active Active
- 2010-07-09 EP EP10797920.5A patent/EP2451981B1/en active Active
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2015
- 2015-11-02 JP JP2015215544A patent/JP2016065872A/ja active Pending
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| Publication number | Publication date |
|---|---|
| EP2451981A1 (en) | 2012-05-16 |
| WO2011006075A1 (en) | 2011-01-13 |
| JP2016065872A (ja) | 2016-04-28 |
| EP2451981A4 (en) | 2013-07-31 |
| JP2012532612A (ja) | 2012-12-20 |
| US20100055695A1 (en) | 2010-03-04 |
| CA2982304A1 (en) | 2011-01-13 |
| US7964356B2 (en) | 2011-06-21 |
| AU2010271271A1 (en) | 2012-01-19 |
| CA2765857C (en) | 2018-03-06 |
| CA2765857A1 (en) | 2011-01-13 |
| EP2451981B1 (en) | 2016-10-19 |
| AU2010271271B2 (en) | 2015-01-29 |
| JP5926178B2 (ja) | 2016-05-25 |
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