ES2433697B1 - Minitransposón miniUIB y usos derivados - Google Patents

Minitransposón miniUIB y usos derivados Download PDF

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Publication number
ES2433697B1
ES2433697B1 ES201230697A ES201230697A ES2433697B1 ES 2433697 B1 ES2433697 B1 ES 2433697B1 ES 201230697 A ES201230697 A ES 201230697A ES 201230697 A ES201230697 A ES 201230697A ES 2433697 B1 ES2433697 B1 ES 2433697B1
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Spain
Prior art keywords
transposon
mini
miniuib
nucleic acid
bacterium
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Active
Application number
ES201230697A
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English (en)
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ES2433697A1 (es
Inventor
Joseph Alexander CHRISTIE OLEZA
Isabel BRUNET GALMÉS
Jorge LALUCAT JO
Balbina NOGALES FERNÁNDEZ
Rafael BOSCH ZARAGOZA
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Universitat de les Illes Balears
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Universitat de les Illes Balears
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Priority to ES201230697A priority Critical patent/ES2433697B1/es
Priority to PCT/ES2013/070294 priority patent/WO2013167784A1/es
Publication of ES2433697A1 publication Critical patent/ES2433697A1/es
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Minitransposón miniUIB y usos derivados.#La presente invención se refiere a un minitransposón que comprende las repeticiones invertidas izquierda y derecha de ISPst9, a su uso para la transferencia de un ácido nucleico o para la mutagénesis aleatoria. Así mismo, la presente invención se refiere a un método para la transposición de un ácido nucleico desde una bacteria dadora a una bacteria receptora que comprende introducir el ácido nucleico a transponer entre las repeticiones invertidas del minitransposón de la invención, introducir el minitransposón en la bacteria dadora y conjugar ambas bacterias dadora y receptora.

Description

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Claims (1)

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ES201230697A 2012-05-09 2012-05-09 Minitransposón miniUIB y usos derivados Active ES2433697B1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201230697A ES2433697B1 (es) 2012-05-09 2012-05-09 Minitransposón miniUIB y usos derivados
PCT/ES2013/070294 WO2013167784A1 (es) 2012-05-09 2013-05-09 Minitransposón miniuib y usos derivados

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201230697A ES2433697B1 (es) 2012-05-09 2012-05-09 Minitransposón miniUIB y usos derivados

Publications (2)

Publication Number Publication Date
ES2433697A1 ES2433697A1 (es) 2013-12-12
ES2433697B1 true ES2433697B1 (es) 2014-09-29

Family

ID=49550190

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201230697A Active ES2433697B1 (es) 2012-05-09 2012-05-09 Minitransposón miniUIB y usos derivados

Country Status (2)

Country Link
ES (1) ES2433697B1 (es)
WO (1) WO2013167784A1 (es)

Also Published As

Publication number Publication date
WO2013167784A1 (es) 2013-11-14
WO2013167784A8 (es) 2014-01-03
ES2433697A1 (es) 2013-12-12

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