ES2122692T3 - Procedimiento de amplificacion de acidos nucleicos en fase solida y equipo de reactivos para su realizacion. - Google Patents

Procedimiento de amplificacion de acidos nucleicos en fase solida y equipo de reactivos para su realizacion.

Info

Publication number
ES2122692T3
ES2122692T3 ES95936616T ES95936616T ES2122692T3 ES 2122692 T3 ES2122692 T3 ES 2122692T3 ES 95936616 T ES95936616 T ES 95936616T ES 95936616 T ES95936616 T ES 95936616T ES 2122692 T3 ES2122692 T3 ES 2122692T3
Authority
ES
Spain
Prior art keywords
solid phase
realization
nucleic acid
acid amplification
amplification procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95936616T
Other languages
English (en)
Inventor
Christine Peponnet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Biodevelopment SAS
Original Assignee
Genset SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genset SA filed Critical Genset SA
Application granted granted Critical
Publication of ES2122692T3 publication Critical patent/ES2122692T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A UN PROCEDIMIENTO DE AMPLIFICACION DE ACIDOS NUCLEICOS EN FASE SOLIDA EN EL QUE SE UTILIZA UN CEBO INMOVILIZADO SOBRE UN SOPORTE SOLIDO TERMORRESISTENTE FUNCIONALIZADO, CARACTERIZADO PORQUE DICHO CEBO ESTA INMOVILIZADO SOBRE EL SOPORTE SOLIDO MEDIANTE UN BRAZO DE CONEXION QUE CONSISTE EN UNA MOLECULA POLIFUNCIONAL QUE ESTABLECE UN ENLACE COVALENTE ENTRE EL SOPORTE SOLIDO Y UN PRIMER GRUPO FUNCIONAL DE DICHA MOLECULA POLIFUNCIONAL, Y ENTRE EL EXTREMO 5'' DE DICHO CEBO Y UN SEGUNDO GRUPO FUNCIONAL DE DICHA MOLECULA POLIFUNCIONAL.
ES95936616T 1994-10-28 1995-10-27 Procedimiento de amplificacion de acidos nucleicos en fase solida y equipo de reactivos para su realizacion. Expired - Lifetime ES2122692T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9412972A FR2726286B1 (fr) 1994-10-28 1994-10-28 Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede

Publications (1)

Publication Number Publication Date
ES2122692T3 true ES2122692T3 (es) 1998-12-16

Family

ID=9468346

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95936616T Expired - Lifetime ES2122692T3 (es) 1994-10-28 1995-10-27 Procedimiento de amplificacion de acidos nucleicos en fase solida y equipo de reactivos para su realizacion.

Country Status (9)

Country Link
US (2) US6274351B1 (es)
EP (1) EP0787205B1 (es)
JP (1) JP4067057B2 (es)
AT (1) ATE169683T1 (es)
DE (1) DE69504094T2 (es)
DK (1) DK0787205T3 (es)
ES (1) ES2122692T3 (es)
FR (1) FR2726286B1 (es)
WO (1) WO1996013609A1 (es)

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US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
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US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
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JP5180845B2 (ja) * 2006-02-24 2013-04-10 カリダ・ジェノミックス・インコーポレイテッド Ｄｎａアレイ上でのハイスループットゲノム配列決定
SG10201405158QA (en) * 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
WO2007140417A2 (en) * 2006-05-31 2007-12-06 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
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Also Published As

Publication number Publication date
DK0787205T3 (da) 1999-05-17
DE69504094T2 (de) 1999-03-11
JPH10507923A (ja) 1998-08-04
EP0787205A1 (fr) 1997-08-06
ATE169683T1 (de) 1998-08-15
FR2726286A1 (fr) 1996-05-03
US6274351B1 (en) 2001-08-14
DE69504094D1 (de) 1998-09-17
EP0787205B1 (fr) 1998-08-12
WO1996013609A1 (fr) 1996-05-09
JP4067057B2 (ja) 2008-03-26
US6277604B1 (en) 2001-08-21
FR2726286B1 (fr) 1997-01-17

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