ES2122692T3 - Procedimiento de amplificacion de acidos nucleicos en fase solida y equipo de reactivos para su realizacion. - Google Patents
Procedimiento de amplificacion de acidos nucleicos en fase solida y equipo de reactivos para su realizacion.Info
- Publication number
- ES2122692T3 ES2122692T3 ES95936616T ES95936616T ES2122692T3 ES 2122692 T3 ES2122692 T3 ES 2122692T3 ES 95936616 T ES95936616 T ES 95936616T ES 95936616 T ES95936616 T ES 95936616T ES 2122692 T3 ES2122692 T3 ES 2122692T3
- Authority
- ES
- Spain
- Prior art keywords
- solid phase
- realization
- nucleic acid
- acid amplification
- amplification procedure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Abstract
LA PRESENTE INVENCION SE REFIERE A UN PROCEDIMIENTO DE AMPLIFICACION DE ACIDOS NUCLEICOS EN FASE SOLIDA EN EL QUE SE UTILIZA UN CEBO INMOVILIZADO SOBRE UN SOPORTE SOLIDO TERMORRESISTENTE FUNCIONALIZADO, CARACTERIZADO PORQUE DICHO CEBO ESTA INMOVILIZADO SOBRE EL SOPORTE SOLIDO MEDIANTE UN BRAZO DE CONEXION QUE CONSISTE EN UNA MOLECULA POLIFUNCIONAL QUE ESTABLECE UN ENLACE COVALENTE ENTRE EL SOPORTE SOLIDO Y UN PRIMER GRUPO FUNCIONAL DE DICHA MOLECULA POLIFUNCIONAL, Y ENTRE EL EXTREMO 5'' DE DICHO CEBO Y UN SEGUNDO GRUPO FUNCIONAL DE DICHA MOLECULA POLIFUNCIONAL.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9412972A FR2726286B1 (fr) | 1994-10-28 | 1994-10-28 | Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2122692T3 true ES2122692T3 (es) | 1998-12-16 |
Family
ID=9468346
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES95936616T Expired - Lifetime ES2122692T3 (es) | 1994-10-28 | 1995-10-27 | Procedimiento de amplificacion de acidos nucleicos en fase solida y equipo de reactivos para su realizacion. |
Country Status (9)
Country | Link |
---|---|
US (2) | US6274351B1 (es) |
EP (1) | EP0787205B1 (es) |
JP (1) | JP4067057B2 (es) |
AT (1) | ATE169683T1 (es) |
DE (1) | DE69504094T2 (es) |
DK (1) | DK0787205T3 (es) |
ES (1) | ES2122692T3 (es) |
FR (1) | FR2726286B1 (es) |
WO (1) | WO1996013609A1 (es) |
Families Citing this family (56)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9624165D0 (en) * | 1996-11-19 | 1997-01-08 | Amdex A S | Use of nucleic acids bound to carrier macromolecules |
DE69837913T2 (de) | 1997-04-01 | 2008-02-07 | Solexa Ltd., Saffron Walden | Verfahren zur vervielfältigung von nukleinsäure |
US6485944B1 (en) * | 1997-10-10 | 2002-11-26 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
US6511803B1 (en) * | 1997-10-10 | 2003-01-28 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
US6322968B1 (en) | 1997-11-21 | 2001-11-27 | Orchid Biosciences, Inc. | De novo or “universal” sequencing array |
US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US6872521B1 (en) * | 1998-06-16 | 2005-03-29 | Beckman Coulter, Inc. | Polymerase signaling assay |
DE19853185C1 (de) * | 1998-11-19 | 2001-01-18 | Guenter Von Kiedrowski | Chemische Evolution durch exponentielle Amplifikation molekularer Matrizen |
DE19848403A1 (de) * | 1998-10-21 | 2000-04-27 | Guenter Von Kiedrowski | Verfahren zur exponentiellen Amplifikation molekularer Matrizen |
US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
WO2001029248A2 (en) * | 1999-10-19 | 2001-04-26 | Bionex, Inc. | Method for amplifying and detecting nucleic acid |
FR2802550B1 (fr) * | 1999-12-17 | 2004-03-05 | Centre Nat Rech Scient | Biopuces, preparation et utilisations |
EP1111068A1 (en) * | 1999-12-21 | 2001-06-27 | LION Bioscience AG | Branched compound for use in nucleic acid detection and analysis reactions |
AU2170901A (en) * | 1999-12-21 | 2001-07-03 | Lion Bioscience Ag | Branched compound for use in nucleic acid detection and analysis reactions |
EP1130121A3 (en) | 2000-01-26 | 2003-04-16 | Nisshinbo Industries Inc. | Immobilized nucleic acid and method for detecting nucleic acid |
JP2004523243A (ja) * | 2001-03-12 | 2004-08-05 | カリフォルニア インスティチュート オブ テクノロジー | 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置 |
DE10224339A1 (de) * | 2002-05-29 | 2003-12-11 | Axaron Bioscience Ag | Verfahren zur hochparallelen Nukleinsäuresequenzierung |
JP4473878B2 (ja) | 2003-01-29 | 2010-06-02 | 454 コーポレーション | 核酸を増幅および配列決定する方法 |
WO2004083429A1 (ja) * | 2003-03-18 | 2004-09-30 | Nec Corporation | Dna断片増幅方法、dna断片の増幅を行う反応装置およびその製造方法 |
EP1641809B2 (en) | 2003-07-05 | 2018-10-03 | The Johns Hopkins University | Method and compositions for detection and enumeration of genetic variations |
US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
KR101077603B1 (ko) * | 2004-01-28 | 2011-10-27 | 삼성전자주식회사 | 카르복실기 또는 아미노기로 코팅된 고체상 물질을 이용한핵산의 증폭 방법 |
ATE463584T1 (de) | 2004-02-19 | 2010-04-15 | Helicos Biosciences Corp | Verfahren zur analyse von polynukleotidsequenzen |
DK2620510T4 (da) | 2005-06-15 | 2020-03-30 | Complete Genomics Inc | Enkeltmolekyle-arrays til genetisk og kemisk analyse |
US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
US7960104B2 (en) * | 2005-10-07 | 2011-06-14 | Callida Genomics, Inc. | Self-assembled single molecule arrays and uses thereof |
GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
JP5180845B2 (ja) * | 2006-02-24 | 2013-04-10 | カリダ・ジェノミックス・インコーポレイテッド | Dnaアレイ上でのハイスループットゲノム配列決定 |
SG10201405158QA (en) * | 2006-02-24 | 2014-10-30 | Callida Genomics Inc | High throughput genome sequencing on dna arrays |
WO2007140417A2 (en) * | 2006-05-31 | 2007-12-06 | Sequenom, Inc. | Methods and compositions for the extraction and amplification of nucleic acid from a sample |
US7910302B2 (en) | 2006-10-27 | 2011-03-22 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
WO2008070375A2 (en) | 2006-11-09 | 2008-06-12 | Complete Genomics, Inc. | Selection of dna adaptor orientation |
US20090105961A1 (en) * | 2006-11-09 | 2009-04-23 | Complete Genomics, Inc. | Methods of nucleic acid identification in large-scale sequencing |
WO2008092213A1 (en) | 2007-02-02 | 2008-08-07 | Genera Biosystems Pty Ltd | Generation of nucleic acid molecules |
GB0712882D0 (en) * | 2007-07-03 | 2007-08-15 | Leicester University Of | Nucleic acid amplification |
EP2191011B1 (en) * | 2007-08-29 | 2017-03-29 | Illumina Cambridge Limited | Method for sequencing a polynucleotide template |
US8518640B2 (en) * | 2007-10-29 | 2013-08-27 | Complete Genomics, Inc. | Nucleic acid sequencing and process |
US7897344B2 (en) * | 2007-11-06 | 2011-03-01 | Complete Genomics, Inc. | Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs |
US20090263872A1 (en) * | 2008-01-23 | 2009-10-22 | Complete Genomics Inc. | Methods and compositions for preventing bias in amplification and sequencing reactions |
US8415099B2 (en) * | 2007-11-05 | 2013-04-09 | Complete Genomics, Inc. | Efficient base determination in sequencing reactions |
WO2009061840A1 (en) * | 2007-11-05 | 2009-05-14 | Complete Genomics, Inc. | Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation |
WO2009073629A2 (en) | 2007-11-29 | 2009-06-11 | Complete Genomics, Inc. | Efficient shotgun sequencing methods |
US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
WO2009097368A2 (en) | 2008-01-28 | 2009-08-06 | Complete Genomics, Inc. | Methods and compositions for efficient base calling in sequencing reactions |
WO2009132028A1 (en) * | 2008-04-21 | 2009-10-29 | Complete Genomics, Inc. | Array structures for nucleic acid detection |
JP5438922B2 (ja) * | 2008-06-25 | 2014-03-12 | 日東電工株式会社 | 核酸の製造方法 |
DE102009012169B3 (de) | 2009-03-06 | 2010-11-04 | Albert-Ludwigs-Universität Freiburg | Vorrichtung und Verfahren zum Herstellen eines Replikats oder eines Derivats aus einem Array von Molekülen und Anwendungen derselben |
CA2757493C (en) | 2009-04-03 | 2018-11-13 | Sequenom, Inc. | Nucleic acid preparation compositions and methods |
US9524369B2 (en) | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
KR20120045909A (ko) * | 2010-11-01 | 2012-05-09 | 엘지전자 주식회사 | 복수 종의 표적 핵산을 실시간으로 검출하는 장치 및 방법 |
US20120152743A1 (en) * | 2010-12-17 | 2012-06-21 | General Electric Company | Method for electroeluting genetic material from dried samples |
DE102011054101A1 (de) | 2011-09-30 | 2013-04-04 | Albert-Ludwigs-Universität Freiburg | Verfahren zur räumlichen Anordnung von Probenfragmenten zur Amplifikation und Immobilisierung für weitere Derivatisierungen |
US10167501B2 (en) * | 2014-06-12 | 2019-01-01 | Auckland Uniservices Limited | Methods and apparatus for quantification of nucleic acid amplification by monitoring impedances |
CA3046659A1 (en) | 2016-12-22 | 2018-06-28 | Illumina, Inc. | Flow cell package and method for making the same |
TW202120693A (zh) * | 2019-08-21 | 2021-06-01 | 美商伊路米納有限公司 | 偵測核苷酸之聚合酶併入的方法 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4849077A (en) * | 1984-08-06 | 1989-07-18 | Akademie Der Wissenschaften Der Ddr | Process for solid phase-sequencing of nucleic acid fragments |
WO1990001546A1 (en) * | 1988-08-05 | 1990-02-22 | Applied Biotechnology, Inc. | Equine herpesvirus-1 vaccine |
WO1991000868A1 (en) * | 1989-07-07 | 1991-01-24 | National Research Development Corporation | Immobilised polynucleotides |
EP0504321B1 (en) * | 1989-12-04 | 1997-05-07 | Microprobe Corporation | Enhanced capture of target nucleic acid by the use of oligonucleotides covalently attached to polymers |
JP3011987B2 (ja) | 1990-10-11 | 2000-02-21 | 旭化成工業株式会社 | 固相化プライマーを用いる逆転写酵素の測定法 |
US5437976A (en) * | 1991-08-08 | 1995-08-01 | Arizona Board Of Regents, The University Of Arizona | Multi-domain DNA ligands bound to a solid matrix for protein and nucleic acid affinity chromatography and processing of solid-phase DNA |
WO1993004199A2 (en) * | 1991-08-20 | 1993-03-04 | Scientific Generics Limited | Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids |
WO1993009250A1 (en) * | 1991-11-01 | 1993-05-13 | Adelaide Children's Hospital | Solid phase amplification process |
NZ246203A (en) * | 1991-12-24 | 1996-09-25 | Tepnel Medical Ltd | Copying of a nucleic acid sequence using a solid support system, apparatus therefor |
MX9300492A (es) * | 1992-01-29 | 1994-07-29 | Hitachi Chemical Co Ltd | Soporte inmovilizado de nucleotido y metodo para su produccion. |
US5344701A (en) * | 1992-06-09 | 1994-09-06 | Minnesota Mining And Manufacturing Company | Porous supports having azlactone-functional surfaces |
US6060288A (en) * | 1994-08-03 | 2000-05-09 | Mosaic Technologies | Method for performing amplification of nucleic acid on supports |
-
1994
- 1994-10-28 FR FR9412972A patent/FR2726286B1/fr not_active Expired - Fee Related
-
1995
- 1995-10-27 ES ES95936616T patent/ES2122692T3/es not_active Expired - Lifetime
- 1995-10-27 WO PCT/FR1995/001422 patent/WO1996013609A1/fr active IP Right Grant
- 1995-10-27 DE DE69504094T patent/DE69504094T2/de not_active Expired - Fee Related
- 1995-10-27 US US08/836,031 patent/US6274351B1/en not_active Expired - Fee Related
- 1995-10-27 AT AT95936616T patent/ATE169683T1/de active
- 1995-10-27 JP JP51436296A patent/JP4067057B2/ja not_active Expired - Fee Related
- 1995-10-27 DK DK95936616T patent/DK0787205T3/da active
- 1995-10-27 EP EP95936616A patent/EP0787205B1/fr not_active Expired - Lifetime
-
1999
- 1999-10-29 US US09/430,035 patent/US6277604B1/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
DK0787205T3 (da) | 1999-05-17 |
DE69504094T2 (de) | 1999-03-11 |
JPH10507923A (ja) | 1998-08-04 |
EP0787205A1 (fr) | 1997-08-06 |
ATE169683T1 (de) | 1998-08-15 |
FR2726286A1 (fr) | 1996-05-03 |
US6274351B1 (en) | 2001-08-14 |
DE69504094D1 (de) | 1998-09-17 |
EP0787205B1 (fr) | 1998-08-12 |
WO1996013609A1 (fr) | 1996-05-09 |
JP4067057B2 (ja) | 2008-03-26 |
US6277604B1 (en) | 2001-08-21 |
FR2726286B1 (fr) | 1997-01-17 |
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Legal Events
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---|---|---|---|
FG2A | Definitive protection |
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