ES2113885T3 - Proteina de cacao recombinante de 21kd y precursor. - Google Patents

Proteina de cacao recombinante de 21kd y precursor.

Info

Publication number
ES2113885T3
ES2113885T3 ES91911055T ES91911055T ES2113885T3 ES 2113885 T3 ES2113885 T3 ES 2113885T3 ES 91911055 T ES91911055 T ES 91911055T ES 91911055 T ES91911055 T ES 91911055T ES 2113885 T3 ES2113885 T3 ES 2113885T3
Authority
ES
Spain
Prior art keywords
pct
precursor
recombinant
cocoa
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91911055T
Other languages
English (en)
Inventor
Margaret Elizabeth Spencer
Rachel Hodge
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wrigley Candy UK
Original Assignee
Mars UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mars UK Ltd filed Critical Mars UK Ltd
Application granted granted Critical
Publication of ES2113885T3 publication Critical patent/ES2113885T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23GCOCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G1/00Cocoa; Cocoa products, e.g. chocolate; Substitutes therefor
    • A23G1/30Cocoa products, e.g. chocolate; Substitutes therefor
    • A23G1/32Cocoa products, e.g. chocolate; Substitutes therefor characterised by the composition containing organic or inorganic compounds
    • A23G1/42Cocoa products, e.g. chocolate; Substitutes therefor characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
    • A23G1/423Cocoa products, e.g. chocolate; Substitutes therefor characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins containing microorganisms, enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23GCOCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G1/00Cocoa; Cocoa products, e.g. chocolate; Substitutes therefor
    • A23G1/02Preliminary treatment, e.g. fermentation of cocoa
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • C12N15/73Expression systems using phage (lambda) regulatory sequences
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23GCOCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G2200/00COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF containing organic compounds, e.g. synthetic flavouring agents
    • A23G2200/02COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF containing organic compounds, e.g. synthetic flavouring agents containing microorganisms, enzymes, probiotics
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Inorganic Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Macromonomer-Based Addition Polymer (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Information Retrieval, Db Structures And Fs Structures Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

HA SIDO IDENTIFICADA UNA PROTEINA 21 KD, Y SU PRECURSOR DE EXPRESION 23 KD, PENSADA PARA SER LA FUENTE DE PRECURSORES DE FRAGANCIA PEPTIDA DE CACAO (TEOBROMINA DE CACAO). CODIFICACION DE GENES QUE HAN SIDO INVESTIGADOS, IDENTIFICADOS Y OBTENIDAS SUS SECUENCIAS, Y PROTEINAS RECOMBINADAS HAN SIDO SINTETIZADAS.
ES91911055T 1990-06-11 1991-06-07 Proteina de cacao recombinante de 21kd y precursor. Expired - Lifetime ES2113885T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB909013017A GB9013017D0 (en) 1990-06-11 1990-06-11 Compounds
PCT/GB1991/000913 WO1991019800A1 (en) 1990-06-11 1991-06-07 RECOMBINANT 21 kD COCOA PROTEIN AND PRECURSOR

Publications (1)

Publication Number Publication Date
ES2113885T3 true ES2113885T3 (es) 1998-05-16

Family

ID=10677435

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91911055T Expired - Lifetime ES2113885T3 (es) 1990-06-11 1991-06-07 Proteina de cacao recombinante de 21kd y precursor.

Country Status (21)

Country Link
US (1) US5668007A (es)
EP (1) EP0586372B1 (es)
JP (1) JPH05507849A (es)
KR (1) KR930701602A (es)
AT (1) ATE161884T1 (es)
AU (1) AU659410B2 (es)
BR (1) BR9106558A (es)
CA (1) CA2084058A1 (es)
DE (1) DE69128647T2 (es)
DK (1) DK0586372T3 (es)
ES (1) ES2113885T3 (es)
FI (1) FI105204B (es)
GB (2) GB9013017D0 (es)
GR (1) GR3026456T3 (es)
HK (1) HK168295A (es)
HU (1) HU216905B (es)
IE (1) IE74903B1 (es)
NO (1) NO307834B1 (es)
PL (3) PL168529B1 (es)
PT (1) PT97897B (es)
WO (1) WO1991019800A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9013016D0 (en) * 1990-06-11 1990-08-01 Mars Uk Ltd Compounds
EP0999283A1 (en) * 1998-11-05 2000-05-10 Societe Des Produits Nestle S.A. Use of DNA identification techniques for the determination of genetic material of cocoa in fermented or roasted beans and chocolate
EP1253200A1 (en) 2001-04-25 2002-10-30 Société des Produits Nestlé S.A. Cocoa polypeptides and their use in the production of cocoa and chocolate flavour
US20030129709A1 (en) * 2001-11-02 2003-07-10 Olga Makarova Method for site-directed mutagenesis of nucleic acid molecules using a single primer
AU2002364587A1 (en) 2001-12-21 2003-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
WO2003060071A2 (en) * 2001-12-21 2003-07-24 Human Genome Sciences, Inc. Albumin fusion proteins
WO2005003296A2 (en) 2003-01-22 2005-01-13 Human Genome Sciences, Inc. Albumin fusion proteins
KR101184391B1 (ko) * 2004-02-09 2013-03-14 휴먼 게놈 사이언시즈, 인코포레이티드 알부민 융합 단백질
CA2732614A1 (en) * 2008-08-01 2010-02-04 Natraceutical, S.A. Obtaining cocoa extracts rich in bioactive peptides with ace and pep enzyme inhibitory activity
CA2784333A1 (en) * 2009-12-23 2011-06-30 Biopolis S.L. Production of bioactive products from cocoa with pep enzyme inhibitory activity and antioxidant and/or anti-neurogenerative activity
EP3668529A4 (en) * 2017-07-20 2021-07-14 Spogen Biotech Inc. BIOACTIVE POLYPEPTIDES TO IMPROVE PLANT PROTECTION, GROWTH AND PRODUCTIVITY

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4910297A (en) * 1987-06-29 1990-03-20 Abi Biotechnology Inc. Alpha-amylase inhibitor
GB9013016D0 (en) * 1990-06-11 1990-08-01 Mars Uk Ltd Compounds

Also Published As

Publication number Publication date
HK168295A (en) 1995-11-10
GB2260327A (en) 1993-04-14
IE911961A1 (en) 1991-12-18
US5668007A (en) 1997-09-16
DE69128647D1 (de) 1998-02-12
AU659410B2 (en) 1995-05-18
ATE161884T1 (de) 1998-01-15
NO924737L (no) 1993-02-08
PL169957B1 (pl) 1996-09-30
GR3026456T3 (en) 1998-06-30
DK0586372T3 (da) 1998-09-07
PT97897B (pt) 1998-10-30
CA2084058A1 (en) 1991-12-12
HUT65581A (en) 1994-07-28
HU216905B (hu) 1999-10-28
FI925612A0 (fi) 1992-12-10
GB9225933D0 (en) 1993-02-17
BR9106558A (pt) 1993-06-22
AU7977491A (en) 1992-01-07
FI925612A (fi) 1992-12-10
NO924737D0 (no) 1992-12-08
EP0586372A1 (en) 1994-03-16
EP0586372B1 (en) 1998-01-07
PT97897A (pt) 1992-03-31
WO1991019800A1 (en) 1991-12-26
KR930701602A (ko) 1993-06-12
HU9203912D0 (en) 1993-03-29
GB2260327B (en) 1994-10-12
GB9013017D0 (en) 1990-08-01
FI105204B (fi) 2000-06-30
PL169138B1 (pl) 1996-06-28
IE74903B1 (en) 1997-08-13
DE69128647T2 (de) 1998-08-06
JPH05507849A (ja) 1993-11-11
PL168529B1 (pl) 1996-02-29
NO307834B1 (no) 2000-06-05

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