ES2060949T3 - Metodo de diferenciacion de leucocitos utilizando una citometria de flujo. - Google Patents
Metodo de diferenciacion de leucocitos utilizando una citometria de flujo.Info
- Publication number
- ES2060949T3 ES2060949T3 ES90305229T ES90305229T ES2060949T3 ES 2060949 T3 ES2060949 T3 ES 2060949T3 ES 90305229 T ES90305229 T ES 90305229T ES 90305229 T ES90305229 T ES 90305229T ES 2060949 T3 ES2060949 T3 ES 2060949T3
- Authority
- ES
- Spain
- Prior art keywords
- litical
- flow cytometry
- agent
- cytometry
- differentiation method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
Abstract
EL INVENTO SE REFIERE A UN AGENTE LITICO Y UN METODO PARA REALIZAR UN COMPUTO DE SUBPOBLACION DE CELULAS DIFERENCIAL, PARA UTILIZAR POR EJEMPLO, EN CITOMETRIA DE CIRCULACION. EL AGENTE LITICO COMPRENDE UN OXIETANOL AROMATICO, UN AMORTIGUADOR ORGANICO CON UN PK DE CASI 8,5 Y UN DETERGENTE NO IONICO. PREFERENTEMENTE, EL OXIETANOL AROMATICO ES 2-FENOXIETANOL. EL METODO DEL AGENTE LITICO PERMITE LA DETERMINACION DIFERENCIAL DE 5 PARTES DE LAS SUBPOBLACIONES DE CELULAS LEUCOCITOS DE UNA MUESTRA COMPLETA DE SANGRE UTILIZANDO LOS METODOS DE LA CITOMETRIA DE CIRCULACION.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US35210689A | 1989-05-15 | 1989-05-15 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2060949T3 true ES2060949T3 (es) | 1994-12-01 |
Family
ID=23383823
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES90305229T Expired - Lifetime ES2060949T3 (es) | 1989-05-15 | 1990-05-15 | Metodo de diferenciacion de leucocitos utilizando una citometria de flujo. |
Country Status (8)
Country | Link |
---|---|
US (1) | US5510267A (es) |
EP (1) | EP0398652B1 (es) |
JP (1) | JPH0726956B2 (es) |
AT (1) | ATE109568T1 (es) |
AU (1) | AU636607B2 (es) |
CA (1) | CA2016699C (es) |
DE (1) | DE69011198T2 (es) |
ES (1) | ES2060949T3 (es) |
Families Citing this family (84)
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JP2565844B2 (ja) * | 1992-02-07 | 1996-12-18 | アボツト・ラボラトリーズ | 細胞溶解処理条件下で異種細胞集団を正確に計数し,感受性に関する格付けを行う方法 |
US6509192B1 (en) * | 1992-02-24 | 2003-01-21 | Coulter International Corp. | Quality control method |
AU2095992A (en) * | 1992-08-11 | 1994-03-24 | Abbott Laboratories | Flow cytometry sheath reagent for elimination of red cells with preservation of nucleated cells |
DE69327775T2 (de) * | 1992-11-19 | 2000-06-21 | Sysmex Corp | Verfahren zur Vorbehandlung für Blutanalyse |
US6812032B1 (en) | 1993-01-21 | 2004-11-02 | Cdc Technologies, Inc. | Apparatus and method for making a plurality of reagent mixtures and analyzing particle distributions of the reagent mixtures |
JP3355038B2 (ja) * | 1994-08-03 | 2002-12-09 | シスメックス株式会社 | 白血球分類方法 |
FR2733835B1 (fr) * | 1995-05-03 | 1997-07-18 | Hycel Groupe Lisabio | Procede et dispositif de detection du point de lyse de globules rouges |
US5639630A (en) * | 1995-05-16 | 1997-06-17 | Bayer Corporation | Method and reagent composition for performing leukocyte differential counts on fresh and aged whole blood samples, based on intrinsic peroxidase activity of leukocytes |
US5817518A (en) * | 1995-12-18 | 1998-10-06 | Coulter International Corp. | Reagent and method for differential determination of leukocytes in blood |
JP2000502446A (ja) * | 1995-12-18 | 2000-02-29 | センター フォー ラボラトリー テクノロジー,インク. | 血液診断装置及び判定方法 |
US6025201A (en) * | 1995-12-28 | 2000-02-15 | Bayer Corporation | Highly sensitive, accurate, and precise automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples |
US5817519A (en) * | 1995-12-28 | 1998-10-06 | Bayer Corporation | Automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples |
US5874310A (en) * | 1997-11-21 | 1999-02-23 | Coulter International Corp. | Method for differentiation of nucleated red blood cells |
US5917584A (en) * | 1997-11-21 | 1999-06-29 | Coulter International Corp. | Method for differentiation of nucleated red blood cells |
US6304328B1 (en) * | 1998-08-14 | 2001-10-16 | Research Foundation Of State University Of New York, The Office Of Technology Licensing, Suny | Non-contact temperature and concentration measurement on liquid surfaces |
US6067157A (en) * | 1998-10-09 | 2000-05-23 | University Of Washington | Dual large angle light scattering detection |
US6423505B1 (en) | 1998-12-03 | 2002-07-23 | Becton Dickinson And Company | Methods and reagents for quantitation of HLA-DR and CD11b expression on peripheral blood cells |
US6200766B1 (en) | 1998-12-03 | 2001-03-13 | Becton Dickinson And Company | Methods and reagents for quantitation of HLA-DR expression on peripheral blood cells |
US6210969B1 (en) * | 1999-04-28 | 2001-04-03 | Coulter International Corp. | Composition and method for differentiation of basophil and eosinophil subpopulations of leukocytes in blood |
US6214625B1 (en) * | 1999-04-28 | 2001-04-10 | Coulter International Corp. | Composition and method for differentiation of basophils and eosinophils in blood |
US6232125B1 (en) | 1999-08-09 | 2001-05-15 | Coulter International Corp. | Method and apparatus for differentiating and enumerating leukocytes |
US7471394B2 (en) * | 2000-08-02 | 2008-12-30 | Honeywell International Inc. | Optical detection system with polarizing beamsplitter |
US8071051B2 (en) | 2004-05-14 | 2011-12-06 | Honeywell International Inc. | Portable sample analyzer cartridge |
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US20060263888A1 (en) * | 2000-06-02 | 2006-11-23 | Honeywell International Inc. | Differential white blood count on a disposable card |
US7553453B2 (en) * | 2000-06-02 | 2009-06-30 | Honeywell International Inc. | Assay implementation in a microfluidic format |
US7242474B2 (en) * | 2004-07-27 | 2007-07-10 | Cox James A | Cytometer having fluid core stream position control |
US6784981B1 (en) * | 2000-06-02 | 2004-08-31 | Idexx Laboratories, Inc. | Flow cytometry-based hematology system |
US7420659B1 (en) * | 2000-06-02 | 2008-09-02 | Honeywell Interantional Inc. | Flow control system of a cartridge |
US7277166B2 (en) * | 2000-08-02 | 2007-10-02 | Honeywell International Inc. | Cytometer analysis cartridge optical configuration |
US7061595B2 (en) * | 2000-08-02 | 2006-06-13 | Honeywell International Inc. | Miniaturized flow controller with closed loop regulation |
WO2002099800A1 (en) | 2001-06-01 | 2002-12-12 | Fujitsu Limited | Magneto-optic recording medium device |
US6869798B2 (en) * | 2003-04-17 | 2005-03-22 | Clinical Diagnostics Solutions, Inc. | Lytic reagent composition for leukocyte differential analysis |
US7630075B2 (en) * | 2004-09-27 | 2009-12-08 | Honeywell International Inc. | Circular polarization illumination based analyzer system |
US20060179731A1 (en) * | 2004-11-02 | 2006-08-17 | Purvis Harrison G | Temporary railing for a building including access opening and associated methods |
FR2883972B1 (fr) * | 2005-03-31 | 2007-11-16 | C2 Diagnostics Sa | Procede pour l'analyse d'un echantillon de sang et appareil et reactif pour sa mise en oeuvre |
JP4965561B2 (ja) | 2005-04-29 | 2012-07-04 | ハネウェル・インターナショナル・インコーポレーテッド | サイトメータ細胞計数及びサイズ測定システム |
US8361410B2 (en) * | 2005-07-01 | 2013-01-29 | Honeywell International Inc. | Flow metered analyzer |
JP2009500612A (ja) * | 2005-07-01 | 2009-01-08 | ハネウェル・インターナショナル・インコーポレーテッド | 流量測定分析器 |
EP1902298B1 (en) * | 2005-07-01 | 2012-01-18 | Honeywell International Inc. | A molded cartridge with 3-d hydrodynamic focusing |
US7843563B2 (en) * | 2005-08-16 | 2010-11-30 | Honeywell International Inc. | Light scattering and imaging optical system |
US7806604B2 (en) * | 2005-10-20 | 2010-10-05 | Honeywell International Inc. | Face detection and tracking in a wide field of view |
WO2007075922A2 (en) * | 2005-12-22 | 2007-07-05 | Honeywell International Inc. | Portable sample analyzer cartridge |
JP2009521683A (ja) * | 2005-12-22 | 2009-06-04 | ハネウェル・インターナショナル・インコーポレーテッド | アナライザーシステム |
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US7674622B2 (en) * | 2006-12-22 | 2010-03-09 | Abbott Laboratories, Inc. | Method for determination of nucleated red blood cells and leukocytes in a whole blood sample in an automated hematology analyzer |
US7449337B2 (en) * | 2007-01-24 | 2008-11-11 | Idexx Laboratories, Inc. | Lytic reagent and method for leukocytes differential in whole blood |
US7816135B2 (en) | 2007-07-05 | 2010-10-19 | Becton, Dickinson And Company | Method of analyzing lymphocytes |
CN101349644B (zh) * | 2007-07-20 | 2012-06-27 | 深圳迈瑞生物医疗电子股份有限公司 | 一种白细胞分类试剂和其使用方法 |
US8102161B2 (en) * | 2007-09-25 | 2012-01-24 | Tdk Corporation | Stable output in a switching power supply by smoothing the output of the secondary coil |
JP2009083382A (ja) * | 2007-10-01 | 2009-04-23 | Brother Ind Ltd | 画像形成装置および画像処理プログラム |
CN101470108B (zh) | 2007-12-24 | 2013-11-27 | 深圳迈瑞生物医疗电子股份有限公司 | 一种对白细胞进行分类的试剂和方法 |
CN101475754A (zh) * | 2008-01-04 | 2009-07-08 | 深圳迈瑞生物医疗电子股份有限公司 | 不对称菁类荧光染料,组合物及在生物样品染色中的用途 |
BRPI0906620A8 (pt) * | 2008-01-31 | 2016-01-19 | Amyris Biotechnologies Inc | Método para quanitificar um cofator e um metabólico da via do mevalonato, extrato de um célula, e, método de fermentação |
JP2009236798A (ja) * | 2008-03-28 | 2009-10-15 | Sysmex Corp | 好塩基球の分類計数方法 |
CN102016577B (zh) | 2008-05-09 | 2015-06-17 | 希森美康株式会社 | 血液分析装置,血液分析方法及溶血剂 |
CN101602762B (zh) * | 2008-06-10 | 2013-10-16 | 深圳迈瑞生物医疗电子股份有限公司 | 不对称菁类化合物、其制备方法及应用 |
US20100034704A1 (en) * | 2008-08-06 | 2010-02-11 | Honeywell International Inc. | Microfluidic cartridge channel with reduced bubble formation |
US8037354B2 (en) | 2008-09-18 | 2011-10-11 | Honeywell International Inc. | Apparatus and method for operating a computing platform without a battery pack |
CN101726579B (zh) * | 2008-10-17 | 2014-06-18 | 深圳迈瑞生物医疗电子股份有限公司 | 血液检测试剂和方法 |
CN101750274B (zh) * | 2008-12-17 | 2014-06-25 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒以及白细胞分类计数的方法 |
CN101988082B (zh) * | 2009-07-31 | 2015-04-08 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法 |
JP4907705B2 (ja) * | 2009-09-29 | 2012-04-04 | シスメックス株式会社 | 尿中赤血球分析用試薬 |
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US8741235B2 (en) | 2011-12-27 | 2014-06-03 | Honeywell International Inc. | Two step sample loading of a fluid analysis cartridge |
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ES2869919T3 (es) * | 2013-03-12 | 2021-10-26 | Abbott Lab | Sistema de análisis de glóbulos blancos |
WO2015073952A1 (en) * | 2013-11-15 | 2015-05-21 | University Of Maryland | Integrated and standalone label and reagent-free microfluidic devices and microsystems for differential white blood cell counts |
US10655188B2 (en) | 2014-06-13 | 2020-05-19 | Q-Linea Ab | Method for determining the identity and antimicrobial susceptibility of a microorganism |
GB201507026D0 (en) | 2015-04-24 | 2015-06-10 | Linea Ab Q | Medical sample transportation container |
NO340218B1 (en) | 2015-07-10 | 2017-03-20 | Marine Harvest Norway As | Device and method for removing parasites on fish |
EP3371591A4 (en) | 2015-11-02 | 2018-11-14 | Chiranjit Deka | Light scatter based apparatus and methods for hematology analysis using only three detectors |
GB2554767A (en) | 2016-04-21 | 2018-04-11 | Q Linea Ab | Detecting and characterising a microorganism |
WO2018226991A1 (en) | 2017-06-07 | 2018-12-13 | Shifamed Holdings, Llc | Intravascular fluid movement devices, systems, and methods of use |
WO2019094963A1 (en) | 2017-11-13 | 2019-05-16 | Shifamed Holdings, Llc | Intravascular fluid movement devices, systems, and methods of use |
EP3746149A4 (en) | 2018-02-01 | 2021-10-27 | Shifamed Holdings, LLC | INTRAVASCULAR BLOOD PUMPS AND METHODS OF USE AND METHODS OF MANUFACTURING |
CA3094373C (en) | 2018-03-30 | 2023-10-17 | Idexx Laboratories, Inc. | Flow cytometer, laser optics assembly thereof, and methods of assembling the same |
WO2021016372A1 (en) | 2019-07-22 | 2021-01-28 | Shifamed Holdings, Llc | Intravascular blood pumps with struts and methods of use and manufacture |
WO2021062265A1 (en) | 2019-09-25 | 2021-04-01 | Shifamed Holdings, Llc | Intravascular blood pump systems and methods of use and control thereof |
EP4168773A1 (en) | 2020-06-17 | 2023-04-26 | Idexx Laboratories, Inc. | Flow cytometer and laser optics assembly thereof |
Family Cites Families (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3874852A (en) * | 1974-07-05 | 1975-04-01 | Coulter Diagnostics Inc | Reagent and method for determining leukocytes and hemoglobin in the blood |
US3962125A (en) * | 1975-01-13 | 1976-06-08 | Coulter Diagnostics, Inc. | Multi-purpose diluent for use in blood analysis by electronic instrumentation of the coulter type |
US4185964A (en) * | 1977-02-08 | 1980-01-29 | Central Laboratories of Associated Maryland Pathologists, Ltd. | Lysing reagent |
US4099917A (en) * | 1977-07-21 | 1978-07-11 | Technicon Instruments Corporation | Process for preparing a cell suspension from blood for discrimination of white blood cells and platelets from other blood particles |
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US4284412A (en) * | 1979-07-13 | 1981-08-18 | Ortho Diagnostics, Inc. | Method and apparatus for automated identification and enumeration of specified blood cell subclasses |
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US4465774A (en) * | 1981-07-16 | 1984-08-14 | Sherwood Medical Company | Standard or control material for glysocylated or total hemoglobin determination |
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US4743678A (en) * | 1983-04-27 | 1988-05-10 | President And Fellows Of Harvard College | Method and products for detection of human T cell leukemia virus |
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JPS6073356A (ja) * | 1983-09-29 | 1985-04-25 | Toa Medical Electronics Co Ltd | 血液分析用試薬 |
US4654312A (en) * | 1984-05-14 | 1987-03-31 | Becton, Dickinson And Company | Lysing agent for analysis of peripheral blood cells |
US4902613A (en) * | 1984-05-14 | 1990-02-20 | Becton, Dickinson And Company | Lysing agent for analysis of peripheral blood cells |
US4751179A (en) * | 1984-05-31 | 1988-06-14 | Coulter Electronics, Inc. | Method and reagents for differential determination of four populations of leukocytes in blood |
US4727020A (en) * | 1985-02-25 | 1988-02-23 | Becton, Dickinson And Company | Method for analysis of subpopulations of blood cells |
US4745071A (en) * | 1985-09-05 | 1988-05-17 | Sequoia-Turner Corporation | Method for the volumetric differentiation of blood cells types |
NL8601000A (nl) * | 1986-04-21 | 1987-11-16 | Jan Greve T H Twente Afdeling | Het gebruik van gepolariseerd licht in stromingscytometrie. |
JPH0635972B2 (ja) * | 1986-11-27 | 1994-05-11 | 東亜医用電子株式会社 | フロ−サイトメトリ−による白血球の分類方法 |
US5039613A (en) * | 1986-11-27 | 1991-08-13 | Toa Medical Electronics Co., Ltd. | Reagents used in a method of classifying leukocytes by flow cytometry |
JPH0782010B2 (ja) * | 1987-10-16 | 1995-09-06 | 東亜医用電子株式会社 | 粒子分析装置用シース液 |
-
1990
- 1990-05-14 CA CA002016699A patent/CA2016699C/en not_active Expired - Lifetime
- 1990-05-15 DE DE69011198T patent/DE69011198T2/de not_active Expired - Fee Related
- 1990-05-15 AU AU55037/90A patent/AU636607B2/en not_active Ceased
- 1990-05-15 AT AT90305229T patent/ATE109568T1/de not_active IP Right Cessation
- 1990-05-15 ES ES90305229T patent/ES2060949T3/es not_active Expired - Lifetime
- 1990-05-15 JP JP2125266A patent/JPH0726956B2/ja not_active Expired - Lifetime
- 1990-05-15 EP EP90305229A patent/EP0398652B1/en not_active Expired - Lifetime
-
1992
- 1992-08-24 US US07/934,282 patent/US5510267A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
ATE109568T1 (de) | 1994-08-15 |
JPH034171A (ja) | 1991-01-10 |
EP0398652A1 (en) | 1990-11-22 |
DE69011198T2 (de) | 1995-01-19 |
CA2016699C (en) | 2003-11-18 |
DE69011198D1 (de) | 1994-09-08 |
AU636607B2 (en) | 1993-05-06 |
CA2016699A1 (en) | 1990-11-15 |
AU5503790A (en) | 1990-11-15 |
JPH0726956B2 (ja) | 1995-03-29 |
EP0398652B1 (en) | 1994-08-03 |
US5510267A (en) | 1996-04-23 |
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