EP4743580A1 - Efficient transgene-free genome mediation in plants in T0 production based on a co-editing strategy - Google Patents

Efficient transgene-free genome mediation in plants in T0 production based on a co-editing strategy

Info

Publication number
EP4743580A1
EP4743580A1 EP24840566.4A EP24840566A EP4743580A1 EP 4743580 A1 EP4743580 A1 EP 4743580A1 EP 24840566 A EP24840566 A EP 24840566A EP 4743580 A1 EP4743580 A1 EP 4743580A1
Authority
EP
European Patent Office
Prior art keywords
mediation
plants
production based
editing strategy
efficient transgene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24840566.4A
Other languages
German (de)
French (fr)
Inventor
Nian Wang
Xiaoen Huang
Hongge Jia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Florida
University of Florida Research Foundation Inc
Original Assignee
University of Florida
University of Florida Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Florida, University of Florida Research Foundation Inc filed Critical University of Florida
Publication of EP4743580A1 publication Critical patent/EP4743580A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8278—Sulfonylurea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01006—Acetolactate synthase (2.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP24840566.4A 2023-07-11 2024-07-11 Efficient transgene-free genome mediation in plants in T0 production based on a co-editing strategy Pending EP4743580A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363526089P 2023-07-11 2023-07-11
PCT/US2024/037618 WO2025015176A1 (en) 2023-07-11 2024-07-11 Efficient transgene-free genome editing in plants in the t0 generation based on a co-editing strategy

Publications (1)

Publication Number Publication Date
EP4743580A1 true EP4743580A1 (en) 2026-05-20

Family

ID=94216446

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24840566.4A Pending EP4743580A1 (en) 2023-07-11 2024-07-11 Efficient transgene-free genome mediation in plants in T0 production based on a co-editing strategy

Country Status (2)

Country Link
EP (1) EP4743580A1 (en)
WO (1) WO2025015176A1 (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA201992615A1 (en) * 2017-09-01 2020-03-10 Институте Оф Генетисс Анд Девелопментал Биологй, Чинесе Асадемй Оф Ссиенсес METHODS FOR ISOLATING CELLS WITHOUT USING TRANSGENIC MARKER SEQUENCES
AU2018338318B2 (en) * 2017-09-21 2022-12-22 Massachusetts Institute Of Technology Systems, methods, and compositions for targeted nucleic acid editing

Also Published As

Publication number Publication date
WO2025015176A1 (en) 2025-01-16

Similar Documents

Publication Publication Date Title
EP4030893C0 (en) ESTABLISHMENT OF A PRODUCTION PLANT FOR AUTOMATED AGRICULTURE WITH CONTROLLED ENVIRONMENT
EP3921145A4 (en) WAVE PLATES ON A CURVED SURFACE AND PROCESS FOR THEIR MANUFACTURE
SE0802203L (en) Hard brazed heat exchanger and method of manufacturing brazed heat exchanger
IL309598A (en) A spray forming substrate with expanded graphite
EP4340590C0 (en) OPTIMIZING A GREENHOUSE ENVIRONMENT
ATE483352T1 (en) CIRCUIT BOARD
EP4643154A4 (en) LIDAR SYSTEM WITH A MODULAR ARRANGEMENT
EP4496865A4 (en) SINGLE-PHASE RETARDED ACID BASED ON A CATIONIC SURFACE ...
EP3848841C0 (en) ABILITY TO FORGET IN A BLOCKCHAIN
JP2012520955A5 (en)
EP4254107C0 (en) OPTIMIZED MANUFACTURING PROCESS BASED ON A 3D MODEL
EP4742886A1 (en) Cucurbitaceae plant resistant to cucumbertaphide-managed yellows
EP4508282A4 (en) Lifting arrangement system with a collared fastening system
EP4010591C0 (en) HEATING A WIND TURBINE SHAFTS
AT505195A3 (en) DEVICE FOR TRANSFERRING STRUCTURES INTO A MASK TO A SUBSTRATE
DK3987928T3 (en) AGRICULTURAL EQUIPMENT WITH A BOOM
JP2017118833A5 (en)
EP4546068A4 (en) PRODUCTION PLANT
CN204762442U (en) Overpass pier afforestation module
EP4104674C0 (en) MODULE FOR PROTECTING A CULTIVATED AREA AGAINST FROST
WO2020101626A3 (en) Modular, passive solar greenhouse structure having hexagon placement made of glass
EP3993960C0 (en) STOPPING A PRODUCTION MACHINE ON A COLLISION-FREE ROAD
CN201036277Y (en) Riveting type flower modeling flowerpot
CN102798911A (en) Component for producing reflector sheets
EP4421307C0 (en) Hydroelectric power plant with a calculator arrangement

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE