EP3610031A4 - Library quantitation and qualification - Google Patents

Library quantitation and qualification Download PDF

Info

Publication number
EP3610031A4
EP3610031A4 EP17905299.8A EP17905299A EP3610031A4 EP 3610031 A4 EP3610031 A4 EP 3610031A4 EP 17905299 A EP17905299 A EP 17905299A EP 3610031 A4 EP3610031 A4 EP 3610031A4
Authority
EP
European Patent Office
Prior art keywords
qualification
library quantitation
quantitation
library
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP17905299.8A
Other languages
German (de)
French (fr)
Other versions
EP3610031A1 (en
Inventor
Douglas A. Amorese
Bin Li
Benjamin G. Schroeder
Richard A. FEKETE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tecan Genomics Inc
Original Assignee
Nugen Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nugen Technologies Inc filed Critical Nugen Technologies Inc
Publication of EP3610031A1 publication Critical patent/EP3610031A1/en
Publication of EP3610031A4 publication Critical patent/EP3610031A4/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/10Signal processing, e.g. from mass spectrometry [MS] or from PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/20Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
EP17905299.8A 2017-04-11 2017-04-11 Library quantitation and qualification Pending EP3610031A4 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2017/027060 WO2018190814A1 (en) 2017-04-11 2017-04-11 Library quantitation and qualification

Publications (2)

Publication Number Publication Date
EP3610031A1 EP3610031A1 (en) 2020-02-19
EP3610031A4 true EP3610031A4 (en) 2020-11-18

Family

ID=63792657

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17905299.8A Pending EP3610031A4 (en) 2017-04-11 2017-04-11 Library quantitation and qualification

Country Status (4)

Country Link
EP (1) EP3610031A4 (en)
JP (1) JP6983906B2 (en)
CN (1) CN110582577A (en)
WO (1) WO2018190814A1 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020197611A1 (en) * 2001-06-21 2002-12-26 Chagovetz Alexander Michael Method for real-time detection and quantification of nucleic acid sequences using fluorescent primers
US20100069250A1 (en) * 2008-08-16 2010-03-18 The Board Of Trustees Of The Leland Stanford Junior University Digital PCR Calibration for High Throughput Sequencing
EP2599879A1 (en) * 2011-11-30 2013-06-05 Agilent Technologies, Inc. A quantitative PCR-based method to predict the efficiency of target enrichment for next-generation sequencing using repetitive DNA elements (lines/sines) as negative controls
US20140186827A1 (en) * 2010-05-14 2014-07-03 Fluidigm, Inc. Assays for the detection of genotype, mutations, and/or aneuploidy

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602005025791D1 (en) * 2004-10-18 2011-02-17 Univ Brandeis PROCESS FOR NUCLEIC ACID AMPLIFICATION
CN104093890B (en) * 2012-01-26 2016-04-20 纽亘技术公司 The composition produced for the enrichment of target nucleotide sequence and efficient library and method
SG11201405274WA (en) * 2012-02-27 2014-10-30 Cellular Res Inc Compositions and kits for molecular counting
US10501783B2 (en) * 2013-12-11 2019-12-10 Qiagen Gmbh Nucleic acid detection and quantification
CN105890722A (en) * 2016-06-13 2016-08-24 常州大学 Method for measuring DNA molecular mass according to resonance principle

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020197611A1 (en) * 2001-06-21 2002-12-26 Chagovetz Alexander Michael Method for real-time detection and quantification of nucleic acid sequences using fluorescent primers
US20100069250A1 (en) * 2008-08-16 2010-03-18 The Board Of Trustees Of The Leland Stanford Junior University Digital PCR Calibration for High Throughput Sequencing
US20140186827A1 (en) * 2010-05-14 2014-07-03 Fluidigm, Inc. Assays for the detection of genotype, mutations, and/or aneuploidy
EP2599879A1 (en) * 2011-11-30 2013-06-05 Agilent Technologies, Inc. A quantitative PCR-based method to predict the efficiency of target enrichment for next-generation sequencing using repetitive DNA elements (lines/sines) as negative controls

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2018190814A1 *

Also Published As

Publication number Publication date
EP3610031A1 (en) 2020-02-19
JP6983906B2 (en) 2021-12-17
JP2020516274A (en) 2020-06-11
CN110582577A (en) 2019-12-17
WO2018190814A1 (en) 2018-10-18

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