EP3370744A4 - Cell cycle block improves efficiency in generating induced pluripotent stem cells - Google Patents

Cell cycle block improves efficiency in generating induced pluripotent stem cells Download PDF

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Publication number
EP3370744A4
EP3370744A4 EP16862739.6A EP16862739A EP3370744A4 EP 3370744 A4 EP3370744 A4 EP 3370744A4 EP 16862739 A EP16862739 A EP 16862739A EP 3370744 A4 EP3370744 A4 EP 3370744A4
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EP
European Patent Office
Prior art keywords
stem cells
cell cycle
pluripotent stem
induced pluripotent
improves efficiency
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP16862739.6A
Other languages
German (de)
French (fr)
Other versions
EP3370744A1 (en
Inventor
Robin Y. SMITH
Marcie A. Glicksman
Nikhat F. ZAIDI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seaport Diagnostics Inc
Original Assignee
Orig3n Inc
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Filing date
Publication date
Application filed by Orig3n Inc filed Critical Orig3n Inc
Publication of EP3370744A1 publication Critical patent/EP3370744A1/en
Publication of EP3370744A4 publication Critical patent/EP3370744A4/en
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/606—Transcription factors c-Myc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Cell Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Reproductive Health (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
EP16862739.6A 2015-11-02 2016-10-27 Cell cycle block improves efficiency in generating induced pluripotent stem cells Withdrawn EP3370744A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562249520P 2015-11-02 2015-11-02
PCT/US2016/059112 WO2017079029A1 (en) 2015-11-02 2016-10-27 Cell cycle block improves efficiency in generating induced pluripotent stem cells

Publications (2)

Publication Number Publication Date
EP3370744A1 EP3370744A1 (en) 2018-09-12
EP3370744A4 true EP3370744A4 (en) 2019-04-17

Family

ID=58662634

Family Applications (1)

Application Number Title Priority Date Filing Date
EP16862739.6A Withdrawn EP3370744A4 (en) 2015-11-02 2016-10-27 Cell cycle block improves efficiency in generating induced pluripotent stem cells

Country Status (6)

Country Link
EP (1) EP3370744A4 (en)
JP (1) JP2018531610A (en)
KR (1) KR20180072817A (en)
CN (1) CN108348556A (en)
IL (1) IL258640A (en)
WO (1) WO2017079029A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107589002B (en) * 2017-09-08 2020-05-29 杭州市妇产科医院 Preparation method of induced pluripotent stem cell chromosome
US20220267736A1 (en) * 2017-12-28 2022-08-25 Kaneka Corporation Pluripotent stem cell aggregation suppressor
CN110016460A (en) * 2018-01-09 2019-07-16 中国农业大学 A kind of efficient chicken embryo tire primary fibroblast G2/M phase method for synchronizing
CN108531447B (en) * 2018-04-13 2021-11-23 上海市生物医药技术研究院 Compound for regulating sperm motility and assisted reproduction and application thereof
CN115531382B (en) * 2021-06-29 2024-01-30 中国科学院生物物理研究所 Use of quinoline thiazolinone derivative Ro-3306 in treatment of beta-coronavirus infection
CN113462638B (en) * 2021-06-30 2022-10-25 呈诺再生医学科技(珠海横琴新区)有限公司 Efficient genetic-modification-free iPSC induction and industrialization monoclonal picking platform and application
WO2023243679A1 (en) * 2022-06-17 2023-12-21 学校法人日本医科大学 Method for producing high-titer viral vector

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010111422A2 (en) * 2009-03-25 2010-09-30 The Salk Institute For Biological Studies Induced pluripotent stem cell generation using two factors and p53 inactivation
WO2011060100A1 (en) * 2009-11-11 2011-05-19 Sanford-Burnham Medical Research Institute Method for generation and regulation of ips cells and compositions thereof
WO2012136841A1 (en) * 2011-04-08 2012-10-11 INSERM (Institut National de la Santé et de la Recherche Médicale) Method for rejuvenating cells

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6033847A (en) * 1995-02-06 2000-03-07 St. Jude Children's Research Hospital InK4c-p18 and InK4d-p19, inhibitors of cyclin-dependent kinases CDK4 and CDK6, and uses thereof
WO1998032847A2 (en) * 1997-01-23 1998-07-30 The Johns Hopkins University School Of Medicine Characterization of the yeast transcriptome
JP2008307007A (en) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag Human pluripotent stem cell induced from human tissue-originated undifferentiated stem cell after birth
US9238797B2 (en) * 2008-07-07 2016-01-19 Takara Bio Inc. Method for production of pluripotent stem cell
CN104278008B (en) * 2013-07-12 2020-08-21 北京宏冠再生医学科技有限公司 Method, kit and application for preparing pluripotent stem cells through small molecule compound treatment
JP6232907B2 (en) * 2013-10-09 2017-11-22 富士レビオ株式会社 Fused cell and production method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010111422A2 (en) * 2009-03-25 2010-09-30 The Salk Institute For Biological Studies Induced pluripotent stem cell generation using two factors and p53 inactivation
WO2011060100A1 (en) * 2009-11-11 2011-05-19 Sanford-Burnham Medical Research Institute Method for generation and regulation of ips cells and compositions thereof
WO2012136841A1 (en) * 2011-04-08 2012-10-11 INSERM (Institut National de la Santé et de la Recherche Médicale) Method for rejuvenating cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KESHI CHEN ET AL: "Gadd45a is a heterochromatin relaxer that enhances iPS cell generation", EMBO REPORTS, vol. 17, no. 11, 4 October 2015 (2015-10-04), pages 1641 - 1656, XP055564950, ISSN: 1469-3178, DOI: 10.15252/embr.201642402 *
See also references of WO2017079029A1 *

Also Published As

Publication number Publication date
JP2018531610A (en) 2018-11-01
CN108348556A (en) 2018-07-31
KR20180072817A (en) 2018-06-29
EP3370744A1 (en) 2018-09-12
WO2017079029A1 (en) 2017-05-11
IL258640A (en) 2018-06-28

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