EP3342486B1 - Device and method for aerosol exposure - Google Patents

Device and method for aerosol exposure Download PDF

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Publication number
EP3342486B1
EP3342486B1 EP17001888.1A EP17001888A EP3342486B1 EP 3342486 B1 EP3342486 B1 EP 3342486B1 EP 17001888 A EP17001888 A EP 17001888A EP 3342486 B1 EP3342486 B1 EP 3342486B1
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EP
European Patent Office
Prior art keywords
aerosol
container
dosing
metering
fluid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP17001888.1A
Other languages
German (de)
French (fr)
Other versions
EP3342486A3 (en
EP3342486A2 (en
Inventor
Krebs Tobias
Heilemann Sascha
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PIETER VAN WEENEN and CO GmbH House of Innovation
Original Assignee
PIETER VAN WEENEN and CO GmbH House of Innovation
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Publication of EP3342486A2 publication Critical patent/EP3342486A2/en
Publication of EP3342486A3 publication Critical patent/EP3342486A3/en
Application granted granted Critical
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D83/00—Containers or packages with special means for dispensing contents
    • B65D83/14—Containers or packages with special means for dispensing contents for delivery of liquid or semi-liquid contents by internal gaseous pressure, i.e. aerosol containers comprising propellant for a product delivered by a propellant
    • B65D83/38—Details of the container body
    • B65D83/388—Details of the container body with means for suspending the aerosol container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B15/00—Details of spraying plant or spraying apparatus not otherwise provided for; Accessories
    • B05B15/20—Arrangements for agitating the material to be sprayed, e.g. for stirring, mixing or homogenising
    • B05B15/25—Arrangements for agitating the material to be sprayed, e.g. for stirring, mixing or homogenising using moving elements, e.g. rotating blades
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B7/00—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas
    • B05B7/02—Spray pistols; Apparatus for discharge
    • B05B7/04—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge
    • B05B7/0416—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing one gas and one liquid
    • B05B7/0483—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing one gas and one liquid with gas and liquid jets intersecting in the mixing chamber
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D83/00—Containers or packages with special means for dispensing contents
    • B65D83/14—Containers or packages with special means for dispensing contents for delivery of liquid or semi-liquid contents by internal gaseous pressure, i.e. aerosol containers comprising propellant for a product delivered by a propellant
    • B65D83/16—Containers or packages with special means for dispensing contents for delivery of liquid or semi-liquid contents by internal gaseous pressure, i.e. aerosol containers comprising propellant for a product delivered by a propellant characterised by the actuating means
    • B65D83/26—Containers or packages with special means for dispensing contents for delivery of liquid or semi-liquid contents by internal gaseous pressure, i.e. aerosol containers comprising propellant for a product delivered by a propellant characterised by the actuating means operating automatically, e.g. periodically
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D83/00—Containers or packages with special means for dispensing contents
    • B65D83/14—Containers or packages with special means for dispensing contents for delivery of liquid or semi-liquid contents by internal gaseous pressure, i.e. aerosol containers comprising propellant for a product delivered by a propellant
    • B65D83/38—Details of the container body
    • B65D83/384—Details of the container body comprising an aerosol container disposed in an outer shell or in an external container
    • B65D83/386—Details of the container body comprising an aerosol container disposed in an outer shell or in an external container actuation occurring by moving the aerosol container relative to the outer shell or external container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D83/00—Containers or packages with special means for dispensing contents
    • B65D83/14—Containers or packages with special means for dispensing contents for delivery of liquid or semi-liquid contents by internal gaseous pressure, i.e. aerosol containers comprising propellant for a product delivered by a propellant
    • B65D83/72—Containers or packages with special means for dispensing contents for delivery of liquid or semi-liquid contents by internal gaseous pressure, i.e. aerosol containers comprising propellant for a product delivered by a propellant with heating or cooling devices, e.g. heat-exchangers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B13/00—Machines or plants for applying liquids or other fluent materials to surfaces of objects or other work by spraying, not covered by groups B05B1/00 - B05B11/00
    • B05B13/02—Means for supporting work; Arrangement or mounting of spray heads; Adaptation or arrangement of means for feeding work
    • B05B13/04—Means for supporting work; Arrangement or mounting of spray heads; Adaptation or arrangement of means for feeding work the spray heads being moved during spraying operation
    • B05B13/0421—Means for supporting work; Arrangement or mounting of spray heads; Adaptation or arrangement of means for feeding work the spray heads being moved during spraying operation with rotating spray heads

Definitions

  • the present invention relates to devices and methods for dispensing aerosol, for example in the context of investigations of biological samples which are to be exposed to an aerosol.
  • aerosols biological effect and activity of airborne substances, gases, mixtures etc. (hereinafter referred to as aerosols) on biological cells, for example in the human or animal respiratory system, is examined. This can be done by exposing a biological sample to be examined to an aerosol, for example arranged on a membrane.
  • the US 7,185,648 B1 discloses an aerosol dosing container which has one or more stirring elements in its aerosol receiving space, which can be moved by means of a drive relative to the inside of the aerosol receiving space in order to mix aerosol present in the aerosol receiving space.
  • the object of the present invention is to provide solutions which simplify and improve investigations in which a sample is exposed to an aerosol.
  • the stirring element is connected to the aerosol container or the container closure, whereby a movement of the aerosol dosing container as a whole can lead to a relative movement between the stirring element and the aerosol.
  • a positive and / or frictional drive element is arranged on an outside of the aerosol container in order to move the aerosol dosing container relative to the aerosol contained therein.
  • the at least one stirring element can have at least one stirring element extending in a direction parallel and / or perpendicular to a longitudinal axis of the aerosol container.
  • the at least one stirring element can be arranged on an inside of the aerosol container and / or on the container closure.
  • the at least one stirring element can have at least two stirring elements, which can be arranged on an inner side of the aerosol closure and / or can extend in a direction parallel to the longitudinal axis of the aerosol container.
  • the at least one stirring element can be formed on an insert arranged in the aerosol receiving space.
  • a magnetic stirring fish that can be moved in the aerosol receiving space can be provided.
  • the magnetic stirrer fish can be designed as a stir bar, cross stirrer, triangular stirrer or tablet stirrer or the like.
  • the drive element can comprise an annular and / or elongate element, which can have a toothed outside.
  • An annular element with a toothed outside can e.g. be a gear or toothed ring.
  • An elongated element with a toothed outside can e.g. be a rack to move the dosing container in the direction of its longitudinal axis (up / down).
  • An aerosol metering arrangement is also disclosed.
  • the movement device can comprise a positive and / or frictional driven element, which can serve for the operative connection with the positive and / or frictional drive element of the aerosol dosing container.
  • the output element can comprise an annular and / or elongate element, which can have a toothed outside.
  • a ring-shaped element with a toothed outside can be a gearwheel which can interact with the gearwheel ring or the toothed rack of the metering container.
  • An elongated element with a toothed outside can be a toothed rack which can interact with the gear ring or the toothed rack of the metering container
  • the actuating device can have a movable actuating element which can be movable in a direction towards a bottom of an aerosol dosing container arranged in the dosing container holder.
  • the aerosol metering arrangement can comprise a fluid supply device which can be used to supply fluid into aerosols dispensed from the aerosol metering container.
  • the aerosol metering arrangement can comprise at least one heating and / or cooling device.
  • a heating and / or cooling device can be arranged in an area below the metering container holder.
  • a heating and / or cooling device can be arranged in and / or on the metering container holder.
  • a device using heated and / or cooled fluid e.g. water, air
  • a device using electrically operating device can be used as the heating and / or cooling device.
  • An aerosol metering system is also disclosed.
  • the at least one stirring element is connected to the aerosol dosing container. To move the at least one stirring element relative to the aerosol, the aerosol dosing container can be moved.
  • a predetermined amount of aerosol can be dispensed from the aerosol dosing container.
  • the step of dispensing a predetermined amount of aerosol from the aerosol dosing container can be carried out after or before the step of moving the at least one stirring element relative to the aerosol.
  • the steps of dispensing a predetermined amount of aerosol from the aerosol dosing container and moving the at least one stirring element relative to the aerosol can be repeated in this order or in reverse order or in alternating order at least twice at predetermined and / or different time intervals.
  • a predetermined amount of fluid can be fed into the aerosol dispensed from the aerosol dosing container.
  • a predetermined amount of fluid can be removed from the aerosol dispensed from the aerosol dosing container.
  • the drawings show an aerosol metering arrangement, generally designated 2, and an exposure module, generally designated 4.
  • the exposure module 4 has one or more sample receiving areas 6, in which biological samples 10 to be examined can be arranged, for example, by means of so-called inserts 8 (e.g. inserts referred to as Transwell®).
  • inserts 8 e.g. inserts referred to as Transwell®
  • nutrient solution 12 for the samples 10 is present below the inserts 8.
  • a sedimentation tube 14 is assigned to each of the sample receiving areas 6, each defining a sedimentation channel 16 that extends between a sedimentation channel outlet 18, which is opposite the respective sample 10, and a sedimentation channel inlet 20, which ends at the end opposite the sedimentation channel outlet 18 lies. Aerosol can be supplied to the respective samples 10 via the sedimentation tubes 14 or through the sedimentation channels 16.
  • the sedimentation tubes 16 can each have a suction opening 22 which provides a fluid connection to the respective sedimentation channel 16. Gaseous components which, for example, are not intended to reach the samples, can be removed via the suction openings 22 from the aerosol flowing through and / or sinking into the respective sedimentation channel 16.
  • the suction openings 22 can each be connected to a suction line 24, which in turn lead to a suction device 26 (for example a pump). (please refer Fig. 4a )
  • a suction pipe 28 (see Fig. 4b and 4c ) are introduced into the respective sedimentation channel 16. In this way, for example, fluid can be sucked off / removed at a predetermined location or area in the sedimentation channel 16.
  • the suction pipe 28 can be guided down (just about) over the surface of a sample 10.
  • the suction tube 28 can be cannula-like, for example.
  • the aerosol metering arrangement 2 comprises one or more metering container holders 30, in each of which an aerosol metering container 32 can be arranged. Preferably corresponds the number of dosing container holders 30 the number of sample receiving areas 6.
  • a dosing container holders 30 and components of the aerosol dosing arrangement 2 used therewith are described below. The statements made with respect to a metering container holder 30 apply accordingly to any further, unless otherwise stated.
  • the dosing container holder 30 comprises a sleeve 34 in which an aerosol dosing container 32 can be received.
  • a non-positive operative connection between the outside of the aerosol dosing container 32 and the inside of the sleeve 34 can be provided. This can be done for example by means of an O-ring 36 at the upper end of the sleeve 34 as shown.
  • the metering container holder 30 also has a drive device 38, which according to the drawings comprises a sleeve 40 arranged on the outside of the sleeve 34 and a drive element 42 arranged on the outside of the sleeve 40.
  • the drive element 42 can be arranged directly on the sleeve 34.
  • the drive element 42 has an outer circumferential surface 44 which is designed for a non-positive and / or positive connection with a correspondingly designed output element 46, which is described below.
  • the outer circumferential surface 44 can have a toothed surface in order to transmit forces and / or movements of an output element 46, which also has a toothing, from the latter to the drive element 42 in order to move the drive element 42 (e.g. rotationally and / or translationally).
  • the drive element 42 can also be referred to as a toothed wheel.
  • the outer circumferential surface 44 can have a surface that is suitable, for example, by coating and / or because of its material, so that forces and / or movements of an output element 46 can be transmitted to the drive element 42 and thus the drive device 38 by frictional engagement in order to move it ( eg rotational and / or translational).
  • the drive element 42 can also be referred to as a friction wheel.
  • movements of the drive device 38 can be transmitted to the sleeve 34 and an aerosol dosing container 32 arranged therein.
  • the drive device 38 and thus the sleeve 34 and an aerosol dosing container 32 arranged therein are supported and movable by means of a device 50.
  • the device 50 can have one or more ball bearings 50, the outer ring of which are connected to the main holder body 48 and the inner ring of which is connected to the sleeves 34 and / or the sleeve 40.
  • the aerosol metering arrangement 2 further comprises a drive device 52 which has an output element 46 which is provided for the operative connection with the drive device 38 and which serves to drive the drive device 38.
  • the output element 46 has an outer peripheral surface 54 which is designed for a non-positive and / or positive connection with a correspondingly configured drive element 42 (see above).
  • the outer peripheral surface 42 may have a toothed surface.
  • the output element 46 can also be referred to as a toothed wheel.
  • the outer peripheral surface 54 may have a surface that e.g. by coating and / or due to its material, so that forces of the output element 46 can be transmitted to a drive element 42 and thus a drive device 38 by frictional engagement in order to move them (e.g. rotationally and / or translationally).
  • the driven element 46 can also be referred to as a friction wheel.
  • the drive device 52 comprises a controllable drive 56, for example an electric motor, which is preferably designed or can be operated as a stepper motor.
  • the output shaft 58 of the drive 56 can be connected directly or by means of a transmission / gearbox to the output element 46 in order to transmit rotations of the output shaft 58 1: 1 or step-down or reduced to the output element 46.
  • the drive 56 is connected to a controller ST via a wired and / or wireless connection V.
  • the connection V can also be designed to supply the drive 56 with energy.
  • a separate energy supply or an energy supply can be provided, which is arranged in or on the aerosol metering arrangement 2.
  • the drive devices 38 and 52 can be designed to translate an aerosol dosing container 32 in one direction essentially parallel to its longitudinal axis as shown.
  • the drive element 42 can e.g. be designed as an elongated drive element extending in a direction parallel to the longitudinal axis of the sleeve 34 or an aerosol dosing container 32 arranged therein.
  • an elongated toothed rack and / or friction surface and / or coating on the outside of the sleeve 34 (or the sleeve 40) extending in the longitudinal direction thereof can be used.
  • a gearwheel or friction wheel can also be used here as the drive element 46, which can be operated in a controlled manner by means of the controlled drive 56 in order to move an aerosol dosing container 32 in a desired manner in a translatory manner (upward and / or downward as shown).
  • a common drive device 52 can be used for a plurality of metering container holders 30 or their drive devices 38, which e.g. has a shared drive 56 and for the various dosing container holders 30 or their drive devices 38 each has an output element 46.
  • the aerosol metering arrangement 2 has an actuating device 60 which comprises a selectively controllable, movable actuating element 62.
  • the actuating element 62 can be moved pneumatically, for example by means of an electric or hydraulic drive or, as shown, via pneumatic connections 64 and 66.
  • the actuating element 62 can be moved in the direction of the arrow 68 towards an aerosol dosing container 32 in order to contact it and thereby actuate it, as explained further below. By moving the actuating element 62 against the direction of arrow 68, the operative connection with the aerosol dosing container 32 and thus its actuation can be terminated.
  • the aerosol metering arrangement 2 can have a cover 72 which can be assigned by means of a joint / swivel mechanism 70.
  • a cover 72 which can be assigned by means of a joint / swivel mechanism 70.
  • aerosol containers 32 can be introduced into metering container holders 30 or removed therefrom.
  • the lid 72 is closed (see 1a and 1b ) Aerosol dosing containers 32 arranged in the dosing container holders 30 can be operated by means of the actuating device 60 and can be moved by means of the drive devices 38 and 52.
  • the aerosol metering arrangement 2 can comprise a heating and / or cooling device 74, which can have channels 76, for example, through which heated and / or cooled fluid (e.g. heated / cooled water, air) can be guided.
  • heated and / or cooled fluid e.g. heated / cooled water, air
  • the aerosol metering arrangement 2 can comprise a further heating and / or cooling device, which is integrated, for example, in the metering container holder 30 (there, for example, arranged in / on the sleeve 34) to an aerosol metering container 32 arranged there to a predetermined temperature or to bring or keep it in a certain temperature range.
  • the aerosol metering arrangement 2 can comprise a fluid supply device 78.
  • the fluid supply device 78 can serve to introduce fluid, in particular air or another gas, into aerosol flowing through and / or sinking through a sedimentation channel 16.
  • the fluid supply device 78 has one or more supply lines 80 which can be connected to a preferably controllable fluid source 82 which can supply the desired fluid and comprises, for example, a pump.
  • the fluid source 82 can also be used together for several sedimentation tubes 14.
  • a fluid supply device 78 also has inlet channels 84, which extend from a corresponding one of the supply lines 80 to a sedimentation tube 14.
  • the sedimentation tubes 14 have openings 86 adjacent to the respective sedimentation channel inlet 20.
  • the free ends of the inlet channels 84, where fluid to be introduced into sedimentation channels 16 can escape, can protrude into the openings 86 or are at least slightly spaced from the openings 86 that fluid can be introduced into the openings 86 and thus into sedimentation channels 16.
  • Such variants make it possible to use the aerosol metering arrangement 2 different types of exposure modules.
  • outlets of the inlet channels 84 and / or the sedimentation tubes 14 can be configured such that fluid can be introduced directly into the sedimentation channel inlets 20.
  • the inlet channels 84 can be connected to the openings 86 or connected to them.
  • An aerosol dosing container 32 comprises an aerosol container 88, which defines an aerosol receiving space 90 and has an aerosol container opening 92.
  • the aerosol container 88 is closed in a fluid-tight manner by means of a container closure 94 attached to the aerosol container opening 92.
  • the container closure 94 comprises a metering valve 96 which, when actuated, releases or transmits a predetermined amount of aerosol contained in the aerosol container 88 via a movable valve outlet 98.
  • the metering valve 96 can be designed in such a way that it is opened by an actuation and closes automatically after a predetermined period of time. Alternatively, the metering valve 96 can be designed in such a way that it is opened by an actuation as long as it remains open, as long as the actuation continues and only closes when the actuation is ended.
  • a metering valve 96 is provided which can be actuated by ensuring a relative movement between metering valve 96 (and in particular its valve outlet 98) and aerosol container 88.
  • the valve outlet 98 of the metering valve 96 contacts the end of a sedimentation tube 14 with the sedimentation channel inlet 20 (or another element which serves as a stop), as a result of which the valve outlet 98 no longer follows the movement of the aerosol container 88 and this occurs Dosing valve 96 opens.
  • the duration of the opening of the metering valve 96 can be controlled by controlling the movement and positioning of the actuating element 62.
  • Aerosol in the aerosol dosing container 32 can comprise particles and gas, in particular propellant gas. During storage and also when arranged in a dosing container holder 30, sedimentation of particles contained in the aerosol can occur in the aerosol dosing container 32 come. This means that the aerosol in the aerosol dosing container 32 does not have a homogeneous, uniform composition (anymore).
  • one or more stirring elements 102 are provided in the aerosol container 88.
  • One or more stirring elements 102 can be arranged on the inside of the container closure 94 and extend from this into the aerosol receiving space 90; preferably in the longitudinal direction of the aerosol container 88 and / or at a slight angle thereto.
  • three stirring elements of the same or different lengths, also with bevelled ends, can be used. (please refer Fig. 9-12 ).
  • one or more stirring elements 102 can also be provided, which, starting from the inside on the bottom 100 of the dosing container 88, extend inwards into the aerosol receiving space 90; preferably in the longitudinal direction of the aerosol container 88 and / or at a slight angle thereto.
  • three stirring elements of the same or different lengths, also with bevelled ends, can be used, for example (see Fig. 13 ).
  • one or more stirring elements 102 can also be provided, which, starting from an inner lateral surface 104 of the dosing container 88, extend inwards into the aerosol receiving space 90; preferably transverse to the longitudinal direction of the aerosol container 88 and / or at a slight angle thereto.
  • three stirring elements of the same or different lengths can be used, even with bevelled ends. (please refer Fig. 14 )
  • stirring elements 102 which can move freely in the aerosol receiving space 90 (see FIG. Fig. 15-16 ).
  • Such stirring elements 102 can be partially magnetic.
  • Stirrer elements 102 that are not freely movable can be attached to the inside of the container closure 94 and / or the inside of the bottom 100 and / or the inner lateral surface 104 of the dosing container 88.
  • stirring elements 102 which are not freely movable can be attached and / or formed on an insert E which can be introduced into the dosing container 88.
  • stirring elements 102 which are not freely movable can be provided by correspondingly shaping the inside of the dosing container 88.
  • the function of the stirring elements 102 is to stir in the aerosol or to mix its components in order to ensure a homogeneous mixing / distribution of aerosol components, particularly after sedimentation.
  • Mixing by means of the stirring elements 102 can be achieved by moving them relative to the aerosol contained in the aerosol container 88.
  • Such movement of an aerosol dosing container 32 can be achieved by manually moving the aerosol dosing container 32.
  • the movement can be achieved by means of the drive devices 38 and 52.
  • the mixing can be achieved by means of the stirring elements 102 if freely moving stirring elements 102 are present in the aerosol receiving space.
  • the freely moving stirring elements 102 (at least initially) will not move, which leads to a relative movement to the aerosol in the aerosol container 88 or its components.
  • stirring elements 102 can be used, which are partially magnetic. Such more magnetic elements 102 can be moved by means of an externally applied magnetic field in order to stir in the aerosol or to mix its components, as in the case of a magnetic stirrer. With aerosol dosing container 32 this can be done per se if a corresponding magnetic field is applied to an aerosol dosing container 32.
  • the aerosol metering arrangement includes 2 shows a magnetic field generating device which can generate a magnetic field in order to move magnetic stirring elements 102 in an aerosol receiving space.
  • Samples 10 are arranged in the exposure module 4 and the exposure module 4 is positioned below the aerosol metering arrangement, as illustrated in the drawings.
  • the cover 72 thereof is opened, whereby the aerosol dosing containers 32 can be inserted into container holders 30 and held there securely.
  • the lid 72 is then closed. This positions the actuating devices 60 with respect to the aerosol dosing container 32 such that the respective dosing valves 96 can be actuated by means of the corresponding actuating elements 62, as described above.
  • the drive device 52 In order to rotate the aerosol dosing containers 32, as described above, in order to mix components of aerosols contained in the respective aerosol container 88 by means of stirring elements 102 present in them, the drive device 52 is operated in a controlled manner in such a way that the aerosol dosing containers 32 in be rotated as desired. It is possible to specify in a controlled manner how long, how often, how fast and / or in which direction the rotation should take place. This can be specified jointly or individually for the different aerosol dosing containers 32.
  • Such a mixing process can take place before each delivery of aerosol from the aerosol metering containers 32 by actuating the respective metering valve 96, even before the first one.
  • the mixing processes with respect to the different aerosol dosing containers 32 can take place simultaneously, with a time delay, overlapping etc.
  • the corresponding aerosol dosing containers 32 are actuated by means of the respective actuating device 60, as described above. It is possible to specify in a controlled manner how long, how often, and / or how quickly an operation should take place in each case. This can be specified jointly or individually for the different aerosol dosing containers 32.
  • the respective metering valve 96 releases a certain amount of aerosol, which reaches the respective sample via a corresponding sedimentation channel.
  • fluid e.g. air
  • the respective (or possibly common) fluid supply device 78 can be introduced into the corresponding sedimentation channel via the respective (or possibly common) fluid supply device 78, for example in order to dilute the aerosol.
  • fluid e.g. air, propellant gas
  • suction opening 22 can be removed from the corresponding sedimentation channel via the respective suction opening 22, for example in order to prevent undesirable effects on the respective sample 10.
  • the aerosol can be dispensed once, several times and until the aerosol is no longer available or is no longer available in such an amount that the predetermined amount of aerosol can be dispensed.
  • One or more mixing processes (“turning aerosol dosing containers”) can be carried out between each or after a predetermined number of aerosol deliveries.
  • the cover 72 is opened in order to remove the aerosol dosing containers 32 and, if necessary, to replace them with others.
  • REFERENCE SIGN LIST REFERENCE SIGN LIST
  • Aerosol dosing arrangement 2nd Exposure module 4th Sampling area 6 Insert 8th sample 10th Nutrient solution 12th Sedimentation tube 14 Sedimentation channel 16 Sedimentation channel outlet 18th Sedimentation channel inlet 20 Suction opening 22 Suction line 24th Suction device 26 Exhaust pipe 28

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  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Containers And Packaging Bodies Having A Special Means To Remove Contents (AREA)

Description

Die vorliegende Erfindung betrifft Vorrichtungen und Verfahren zur Abgabe von Aerosol, beispielsweise im Rahmen von Untersuchungen biologischer Proben, die einem Aerosol ausgesetzt werden sollen.The present invention relates to devices and methods for dispensing aerosol, for example in the context of investigations of biological samples which are to be exposed to an aerosol.

HINTERGRUND DER ERFINDUNGBACKGROUND OF THE INVENTION

Im Rahmen der Abschätzung von Gesundheitsrisiken wird die biologische Wirkung und Aktivität von luftgetragenen Substanzen, Gasen, Mischungen etc. (im Folgenden als Aerosole bezeichnet) auf biologische Zellen, beispielsweise im menschlichen oder tierischen respiratorischen System, untersucht. Dies kann erfolgen, indem eine zu untersuchende biologische Probe beispielsweise auf einer Membran angeordnet einem Aerosol ausgesetzt wird.As part of the assessment of health risks, the biological effect and activity of airborne substances, gases, mixtures etc. (hereinafter referred to as aerosols) on biological cells, for example in the human or animal respiratory system, is examined. This can be done by exposing a biological sample to be examined to an aerosol, for example arranged on a membrane.

Die US 7,185,648 B1 offenbart einen Aerosol-Dosierbehälter, der in seinem Aerosol-Aufnahmeraum eines oder mehrere Rührelemente aufweist, welche mittels eines Antriebs relativ zu dem den Innenseiten des Aerosol-Aufnahmeraumes bewegt werden kann, um im Aerosol-Aufnahmeraum vorhandenes Aerosol zu vermischen.The US 7,185,648 B1 discloses an aerosol dosing container which has one or more stirring elements in its aerosol receiving space, which can be moved by means of a drive relative to the inside of the aerosol receiving space in order to mix aerosol present in the aerosol receiving space.

AUFGABETASK

Der vorliegenden Erfindung liegt die Aufgabe zugrunde, Lösungen bereitzustellen, welche Untersuchungen, bei denen einer Probe einem Aerosol ausgesetzt wird, vereinfachen und verbessern.The object of the present invention is to provide solutions which simplify and improve investigations in which a sample is exposed to an aerosol.

KURZBESCHREIBUNG DER ERFINDUNGBRIEF DESCRIPTION OF THE INVENTION

Diese Aufgabe wird durch die Gegenstände gemäß den unabhängigen Ansprüchen gelöst. Die abhängigen Ansprüche beschreiben Varianten derselben.This object is solved by the objects according to the independent claims. The dependent claims describe variants of the same.

So ist ein Aerosol-Dosierbehälter zur dosierten Abgabe von Aerosol offenbart. Der Aerosol-Dosierbehälter kann umfassen:

  • einen zur Aufnahme von Aerosol vorgesehenen Aerosol-Behälter, der einen Aerosol-Aufnahmeraum definiert und eine Aerosol-Behälter-Öffnung aufweist,
  • einen zum fluiddichten Verschließen der Aerosol-Behälter-Öffnung vorgesehenen Behälter-Verschluss, der ein betätigbares Dosierventil zur dosierten Abgabe von im Aerosol-Aufnahmeraum enthaltenen Aerosol,
  • wenigstens ein sich im Aerosol-Aufnahmeraum erstreckendes Rührelement, welches relativ zu im Aerosol-Aufnahmeraum enthaltenem Aerosol bewegbar ist, um dieses zu verrühren.
For example, an aerosol dosing container for the metered delivery of aerosol is disclosed. The aerosol dispenser can include:
  • an aerosol container intended for receiving aerosol, which defines an aerosol receiving space and has an aerosol container opening,
  • a container closure provided for the fluid-tight closing of the aerosol container opening, which has an actuatable metering valve for the metered delivery of aerosol contained in the aerosol receiving space,
  • at least one stirring element, which extends in the aerosol receiving space and is movable relative to the aerosol contained in the aerosol receiving space in order to stir it.

Das Rührelement ist mit dem Aerosol-Behälter bzw. dem Behälter-Verschluss verbunden sein, wobei eine Bewegung des Aerosol-Dosierbehälters insgesamt zu einer Relativbewegung zwischen Rührelement und Aerosol führen kann.The stirring element is connected to the aerosol container or the container closure, whereby a movement of the aerosol dosing container as a whole can lead to a relative movement between the stirring element and the aerosol.

An einer Außenseite des Aerosol-Behälters ist ein form- und/oder reibschlüssiges Antriebselement angeordnet sein, um den Aerosol-Dosierbehälter relativ zu darin enthaltenem Aerosol zu bewegen.A positive and / or frictional drive element is arranged on an outside of the aerosol container in order to move the aerosol dosing container relative to the aerosol contained therein.

Das wenigstens eine Rührelement kann wenigstens einen sich in einer Richtung parallel und/oder senkrecht zu einer Längsachse des Aerosol-Behälters erstreckenden Rührelement aufweisen.The at least one stirring element can have at least one stirring element extending in a direction parallel and / or perpendicular to a longitudinal axis of the aerosol container.

Der wenigstens eine Rührelement kann an einer Innenseite des Aerosol-Behälters und/oder am Behälter-Verschluss angeordnet sein.The at least one stirring element can be arranged on an inside of the aerosol container and / or on the container closure.

Das wenigstens eine Rührelement kann wenigstens zwei Rührelemente aufweisen, die an einer Innerseite des Aerosol-Verschlusses angeordnet sein können und/oder sich in einer Richtung parallel zur Längsachse des Aerosol-Behälters erstrecken können.The at least one stirring element can have at least two stirring elements, which can be arranged on an inner side of the aerosol closure and / or can extend in a direction parallel to the longitudinal axis of the aerosol container.

Das wenigstens eine Rührelement kann an einem im Aerosol-Aufnahmeraum angeordneten Einsatz ausgebildet sein.The at least one stirring element can be formed on an insert arranged in the aerosol receiving space.

Zusätzlich zu dem wenigstens einen Rührelement kann ein im Aerosol-Aufnahmeraum bewegbar angeordneter Magnetrührfisch vorgesehen sein.In addition to the at least one stirring element, a magnetic stirring fish that can be moved in the aerosol receiving space can be provided.

Der Magnetrührfisch kann als Rührstäbchen, Kreuzrührer, Dreiecksrührer oder Tablettenrührer oder dergleichen ausgebildet sein.The magnetic stirrer fish can be designed as a stir bar, cross stirrer, triangular stirrer or tablet stirrer or the like.

Das Antriebselement kann ein ringförmiges und/oder längliches Element umfassen, das eine gezahnte Außenseite aufweisen kann.The drive element can comprise an annular and / or elongate element, which can have a toothed outside.

Ein ringförmiges Element mit gezahnter Außenseite kann z.B. ein Zahnrad oder gezahnter Ring sein.An annular element with a toothed outside can e.g. be a gear or toothed ring.

Ein längliches Element mit gezahnter Außenseite kann z.B. eine Zahnstange sein, um den Dosierbehälter in Richtung seiner Längsachse (nach oben/unten) zu bewegen.An elongated element with a toothed outside can e.g. be a rack to move the dosing container in the direction of its longitudinal axis (up / down).

Ferner ist eine Aerosol-Dosieranordnung offenbart.An aerosol metering arrangement is also disclosed.

Die Aerosol-Dosieranordnung umfasst:

  • einen erfindungsgemäßen, d.h. hier weiter oben offenbarten Aerosol-Dosierbehälter,
  • eine Dosierbehälter-Halterung zur Anordnung der Aerosol-Dosierbehälter zur dosierten Abgabe von Aerosol durch Betätigung des Dosierventils des Aerosol-Dosierbehälters,
  • eine Betätigungsvorrichtung zur Betätigung der Dosierventils des Aerosol-Dosierbehälters,
  • eine steuerbare Bewegungsvorrichtung, um den Aerosol-Dosierbehälter gesteuert zu bewegen.
The aerosol dispensing arrangement includes:
  • an aerosol dosing container according to the invention, ie disclosed here above,
  • a dosing container holder for arranging the aerosol dosing container for the dosed delivery of aerosol by actuating the dosing valve of the aerosol dosing container,
  • an actuating device for actuating the metering valve of the aerosol metering container,
  • a controllable movement device to move the aerosol dosing container in a controlled manner.

Die Bewegungsvorrichtung kann ein form- und/oder reibschlüssiges Abtriebselement vorgesehen umfassen, welches zur Wirkverbindung mit dem form- und/oder reibschlüssiges Antriebselement des Aerosol-Dosierbehälters dienen kann.The movement device can comprise a positive and / or frictional driven element, which can serve for the operative connection with the positive and / or frictional drive element of the aerosol dosing container.

Das Abtriebselement kann ein ringförmiges und/oder längliches Element umfassen, das eine gezahnte Außenseite aufweisen kann.The output element can comprise an annular and / or elongate element, which can have a toothed outside.

Ein ringförmiges Element mit gezahnter Außenseite kann ein Zahnrad sein, das mit dem Zahnradring oder der Zahnstange des Dosierbehälters zusammenwirken kann.A ring-shaped element with a toothed outside can be a gearwheel which can interact with the gearwheel ring or the toothed rack of the metering container.

Ein längliches Element mit gezahnter Außenseite kann eine Zahnstange sein, die mit dem Zahnradring oder der Zahnstange des Dosierbehälters zusammenwirken kannAn elongated element with a toothed outside can be a toothed rack which can interact with the gear ring or the toothed rack of the metering container

Die Betätigungsvorrichtung kann ein bewegbares Betätigungselement aufweisen, das in einer Richtung hin zu einem Boden eines in der Dosierbehälter-Halterung angeordneten Aerosol-Dosierbehälters bewegbar sein kann.The actuating device can have a movable actuating element which can be movable in a direction towards a bottom of an aerosol dosing container arranged in the dosing container holder.

Die Aerosol-Dosieranordnung kann eine Fluidzufuhreinrichtung umfassen, welche zur Zufuhr von Fluid in vom Aerosol-Dosierbehälter abgegeben Aerosols dienen kann.The aerosol metering arrangement can comprise a fluid supply device which can be used to supply fluid into aerosols dispensed from the aerosol metering container.

Die Aerosol-Dosieranordnung kann wenigstens eine Heiz- und/oder Kühlvorrichtung umfassen.The aerosol metering arrangement can comprise at least one heating and / or cooling device.

Eine Heiz- und/oder Kühlvorrichtung kann in einem Bereich unterhalb der Dosierbehälter-Halterung angeordnet sein.A heating and / or cooling device can be arranged in an area below the metering container holder.

Eine Heiz- und/oder Kühlvorrichtung kann in und/oder an der Dosierbehälter-Halterung angeordnet sein.A heating and / or cooling device can be arranged in and / or on the metering container holder.

Als Heiz- und/oder Kühlvorrichtung kann eine erwärmtes und/oder gekühltes Fluid (z.B. Wasser, Luft) verwendende Vorrichtung und/oder eine elektrisch arbeitende Vorrichtung verwendet werden.A device using heated and / or cooled fluid (e.g. water, air) and / or an electrically operating device can be used as the heating and / or cooling device.

Ferner ist ein Aerosol-Dosiersystem offenbart.An aerosol metering system is also disclosed.

Das Aerosol-Dosiersystem umfasst:

  • eine erfindungsgemäße, d.h. hier weiter oben genannte Aerosol-Dosieranordnung, und
  • ein Expositionsmodul mit einem Sedimentationsrohr, um von der Aerosol-Dosieranordnung abgegebenes Aerosol einer Probe zuzuführen, wobei
  • das Sedimentationsrohr eine Absaugöffnung zum Absaugen von Fluid aus dem Sedimentationsrohr aufweist.
The aerosol dosing system includes:
  • an aerosol metering arrangement according to the invention, ie mentioned above, and
  • an exposure module with a sedimentation tube to supply aerosol dispensed by the aerosol metering arrangement to a sample, wherein
  • the sedimentation tube has a suction opening for extracting fluid from the sedimentation tube.

Ferner ist ein Verfahren zum Vermischen von Bestandteilen eines Aerosols in einem erfindungsgemäßen Aerosol-Dosierbehälter mit wenigstens einem sich einem Aerosol-Aufnahmeraum des Aerosol-Dosierbehälters erstreckenden Rührelement offenbart.Furthermore, a method for mixing constituents of an aerosol in an aerosol dosing container according to the invention with at least one stirring element extending over an aerosol receiving space of the aerosol dosing container is disclosed.

Das Verfahren umfasst:

  • Bereitstellen des erfindungsgemäßen Aerosol-Dosierbehälters mit einem im Aerosol-Aufnahmeraum enthaltenen Aerosol,
  • Bewegen des Aerosol-Dosierbehälters und damit des wenigstens einen Rührelements relativ zum Aerosol, um dadurch dessen Bestandteile zu Vermischen.
The process includes:
  • Providing the aerosol dosing container according to the invention with an aerosol contained in the aerosol receiving space,
  • Moving the aerosol dosing container and thus the at least one stirring element relative to the aerosol, to thereby mix its components.

Das wenigstens eine Rührelement ist mit dem Aerosol-Dosierbehälter verbunden. Dabei kann zum Bewegen des wenigstens einen Rührelements relativ zum Aerosol der Aerosol-Dosierbehälter bewegt werden.The at least one stirring element is connected to the aerosol dosing container. To move the at least one stirring element relative to the aerosol, the aerosol dosing container can be moved.

Es kann eine vorbestimmte Menge Aerosol aus dem Aerosol-Dosierbehälter abgegeben werden.A predetermined amount of aerosol can be dispensed from the aerosol dosing container.

Der Schritt, eine vorbestimmte Menge Aerosol aus dem Aerosol-Dosierbehälter abzugeben, kann nach oder vor dem Schritt, das wenigstens eine Rührelement relativ zum Aerosol zu bewegen, durchgeführt werden.The step of dispensing a predetermined amount of aerosol from the aerosol dosing container can be carried out after or before the step of moving the at least one stirring element relative to the aerosol.

Die Schritte, eine vorbestimmte Menge Aerosol aus dem Aerosol-Dosierbehälter abzugeben, und das wenigstens eine Rührelement relativ zum Aerosol zu bewegen, können in dieser Reichenfolge oder in umgekehrter Reihenfolge oder in abwechselnder Reihenfolge wenigstens zweimal in vorbestimmten und/oder unterschiedlichen zeitlichen Abständen wiederholt werden.The steps of dispensing a predetermined amount of aerosol from the aerosol dosing container and moving the at least one stirring element relative to the aerosol can be repeated in this order or in reverse order or in alternating order at least twice at predetermined and / or different time intervals.

In das aus dem Aerosol-Dosierbehälter abgegebene Aerosol kann eine vorbestimmte Menge an Fluid zugeführt werden.A predetermined amount of fluid can be fed into the aerosol dispensed from the aerosol dosing container.

Aus dem aus dem Aerosol-Dosierbehälter abgegebenen Aerosol kann eine vorbestimmte Menge an Fluid entfernt werden.A predetermined amount of fluid can be removed from the aerosol dispensed from the aerosol dosing container.

KURZBESCHREIBUNG DER ZEICHNUNGENBRIEF DESCRIPTION OF THE DRAWINGS

Im Folgenden werden Ausführungsformen der vorliegenden Erfindung anhand der Zeichnungen beschrieben.

Fig. 1a und 1b
zeigen schematische Ansichten eines Aerosol-Dosiersystems mit einer Aerosol-Dosieranordnung in geschlossenem Zustand und einem Expositionsmodul;
Fig. 2a und 2b
zeigen schematische Ansichten des Aerosol-Dosiersystems von Fig. 1 mit der Aerosol-Dosieranordnung in geöffnetem Zustand;
Fig. 3
zeigt eine schematische Schnittansicht des Aerosol-Dosiersystems von Fig. 1;
Fig. 4a und 4b
zeigen eine schematische Ansicht im Bereich eines Sedimentationsrohres des Expositionsmoduls von Fig. 1 mit Absaugung;
Fig. 4c
zeigt eine schematische Darstellung einer Absaugung in einem Sedimentationsrohr;
Fig. 5
zeigt eine schematische Schnittansicht der Aerosol-Dosieranordnung von Fig. 1;
Fig. 6
zeigt eine schematische Schnittansicht der Aerosol-Dosieranordnung von Fig. 1 im Bereich einer Dosierbehälter-Halterung;
Fig. 7
zeigt eine weitere schematische Schnittansicht der Aerosol-Dosieranordnung von Fig. 1;
Fig. 8
zeigt eine schematische Schnittansicht einer Heiz- und/oder Kühlvorrichtung;
Fig. 9 bis 16
zeigen schematische Darstellungen von Aerosol-Dosierbehältem mit unterschiedlichen Rührelementen.
Embodiments of the present invention are described below with reference to the drawings.
1a and 1b
show schematic views of an aerosol metering system with an aerosol metering arrangement in the closed state and an exposure module;
2a and 2b
show schematic views of the aerosol dosing system of Fig. 1 with the aerosol metering arrangement in the open state;
Fig. 3
shows a schematic sectional view of the aerosol metering system of FIG Fig. 1 ;
4a and 4b
show a schematic view in the area of a sedimentation tube of the exposure module of Fig. 1 with suction;
Fig. 4c
shows a schematic representation of a suction in a sedimentation tube;
Fig. 5
shows a schematic sectional view of the aerosol metering arrangement of FIG Fig. 1 ;
Fig. 6
shows a schematic sectional view of the aerosol metering arrangement of FIG Fig. 1 in the area of a dosing container holder;
Fig. 7
shows a further schematic sectional view of the aerosol metering arrangement of FIG Fig. 1 ;
Fig. 8
shows a schematic sectional view of a heating and / or cooling device;
9 to 16
show schematic representations of aerosol dosing containers with different stirring elements.

Auf Zeichnungen, die mehrere mit einer Zeichnungsnummer und Kleinbuchstaben bezeichnete Darstellungen umfassen (z.B. Fig. 1), kann zusammenfassend mit der jeweiligen Zeichnungsnummer verwiesen werden.On drawings that include multiple representations labeled with a drawing number and lowercase letters (e.g. Fig. 1 ), can be summarized with the respective drawing number.

In den Zeichnungen haben wenigstens im Wesentlichen funktionsgleiche Elemente gleiche Bezugszeichen. Ferner gelten für verschiedene Ausführungsformen gemachten Ausführungen auch für alle anderen Ausführungsformen, sofern nichts anderes gesagt wird. Ferner wird im Folgenden auf alle Zeichnungen insgesamt Bezug genommen, sofern nicht anders angegeben oder auf bestimmte Zeichnungen verwiesen wird.In the drawings, at least essentially functionally identical elements have the same reference symbols. Furthermore, statements made for various embodiments also apply to all other embodiments, unless stated otherwise. Furthermore, reference is made in the following to all drawings as a whole, unless stated otherwise or to specific drawings.

BESCHREIBUNG BEVORZUGTER AUSFÜHRUNGSFORMENDESCRIPTION OF PREFERRED EMBODIMENTS

Die Zeichnungen zeigen eine insgesamt mit 2 bezeichnete Aerosol-Dosieranordnung und ein insgesamt mit 4 bezeichnetes Expositionsmodul.The drawings show an aerosol metering arrangement, generally designated 2, and an exposure module, generally designated 4.

ExpositionsmodulExposure module

Das Expositionsmodul 4 weist einen oder mehrere Probenaufnahmebereiche 6 auf, in denen beispielsweise mittels sogenannter Inserts 8 (z.B. als Transwell® bezeichnete Inserts) zu untersuchenden biologische Proben 10 angeordnet werden können. In den Probenaufnahmebereichen 6 kann z.B. unterhalb der Inserts 8 Nährlösung 12 für die Proben 10 vorhanden sein.The exposure module 4 has one or more sample receiving areas 6, in which biological samples 10 to be examined can be arranged, for example, by means of so-called inserts 8 (e.g. inserts referred to as Transwell®). In the sample receiving areas 6 e.g. nutrient solution 12 for the samples 10 is present below the inserts 8.

Den Probenaufnahmebereichen 6 ist jeweils eine Sedimentationsröhre 14 zugeordnet, die jeweils einen Sedimentationskanal 16 definiert, der sich zwischen einem Sedimentationskanal-Auslass 18, der der jeweiligen Probe 10 gegenüberliegt, und einem Sedimentationskanal-Einlass 20 erstreckt, der am dem Sedimentationskanal-Auslass 18 gegenüberliegenden Ende liegt. Über die Sedimentationsröhren 14 bzw. durch die Sedimentationskanäle 16 kann den jeweiligen Proben 10 Aerosol zugeführt werden kann.A sedimentation tube 14 is assigned to each of the sample receiving areas 6, each defining a sedimentation channel 16 that extends between a sedimentation channel outlet 18, which is opposite the respective sample 10, and a sedimentation channel inlet 20, which ends at the end opposite the sedimentation channel outlet 18 lies. Aerosol can be supplied to the respective samples 10 via the sedimentation tubes 14 or through the sedimentation channels 16.

Die Sedimentationsröhren 16 können je eine Absaugöffnung 22 aufweisen, die eine Fluidverbindung hin zum jeweiligen Sedimentationskanal 16 bereitstellt. Über die Absaugöffnungen 22 können aus den jeweiligen Sedimentationskanal 16 durchströmendem und/oder darin absinkenden Aerosol gasförmige Bestandteile, die beispielsweise nicht zu den Proben gelangen sollen, entfernt werden. Hierfür können die Absaugöffnungen 22 jeweils mit einer Absaugleitung 24 verbunden sein, die wiederum zu einer Absaugvorrichtung 26 (z.B. eine Pumpe) führen. (siehe Fig. 4a)The sedimentation tubes 16 can each have a suction opening 22 which provides a fluid connection to the respective sedimentation channel 16. Gaseous components which, for example, are not intended to reach the samples, can be removed via the suction openings 22 from the aerosol flowing through and / or sinking into the respective sedimentation channel 16. For this purpose, the suction openings 22 can each be connected to a suction line 24, which in turn lead to a suction device 26 (for example a pump). (please refer Fig. 4a )

Über die Absaugöffnungen 22 kann auch ein Absaugrohr 28 (siehe Fig. 4b und 4c) in den jeweiligen Sedimentationskanal 16 eingebracht werden. Auf diese Weise kann beispielsweise an einer vorbestimmten Stelle oder Bereich im Sedimentationskanal 16 Fluid abgesaugt/entfernt werden. Das Absaugrohr 28 kann bis hinunter (knapp) über die Oberfläche einer Probe 10 geführt werden. Als Absaugrohr 28 kann z.B. kanülenartig sein.A suction pipe 28 (see Fig. 4b and 4c ) are introduced into the respective sedimentation channel 16. In this way, for example, fluid can be sucked off / removed at a predetermined location or area in the sedimentation channel 16. The suction pipe 28 can be guided down (just about) over the surface of a sample 10. The suction tube 28 can be cannula-like, for example.

Aerosol-DosieranordnungAerosol dosing arrangement

Die Aerosol-Dosieranordnung 2 umfasst eine oder mehrere Dosierbehälter-Halterungen 30, in denen jeweils ein Aerosol-Dosierbehälter 32 angeordnet werden kann. Vorzugsweise entspricht die Anzahl der Dosierbehälter-Halterungen 30 der Anzahl der Probenaufnahmebereiche 6. Im Folgenden werden eine Dosierbehälter-Halterungen 30 und damit verwendete Komponenten der Aerosol-Dosieranordnung 2 beschrieben. Die bezüglich einer Dosierbehälter-Halterungen 30 gemachten Ausführungen gelten entsprechend für jeder weitere, sofern nichts anderes angegeben ist.The aerosol metering arrangement 2 comprises one or more metering container holders 30, in each of which an aerosol metering container 32 can be arranged. Preferably corresponds the number of dosing container holders 30 the number of sample receiving areas 6. A dosing container holders 30 and components of the aerosol dosing arrangement 2 used therewith are described below. The statements made with respect to a metering container holder 30 apply accordingly to any further, unless otherwise stated.

Die Dosierbehälter-Halterung 30 umfasst eine Hülse 34, in der ein Aerosol-Dosierbehälter 32 aufgenommen werden kann. Um einen Aerosol-Dosierbehälter 32 in einer Hülse 34 zu positionieren und z.B. vor Verrutschen zu sichern, kann eine kraftschlüssige Wirkverbindung zwischen der Außenseite des Aerosol-Dosierbehälters 32 und der Innenseite der Hülse 34 vorgesehen sein. Dies kann beispielsweise mittels eines O-Rings 36 am darstellungsgemäß oberen Ende der Hülse 34 erfolgen.The dosing container holder 30 comprises a sleeve 34 in which an aerosol dosing container 32 can be received. In order to position an aerosol dosing container 32 in a sleeve 34 and e.g. To prevent slipping, a non-positive operative connection between the outside of the aerosol dosing container 32 and the inside of the sleeve 34 can be provided. This can be done for example by means of an O-ring 36 at the upper end of the sleeve 34 as shown.

Die Dosierbehälter-Halterung 30 weist ferner jeweils eine Antriebsvorrichtung 38 auf, die gemäß den Zeichnungen eine an der Außenseite der Hülse 34 angeordnete Hülse 40 und ein an der Außenseite der Hülse 40 angeordnete Antriebselement 42 umfassen. Bei weiteren Varianten kann das Antriebselement 42 unmittelbar an der Hülse 34 angeordnet sein.The metering container holder 30 also has a drive device 38, which according to the drawings comprises a sleeve 40 arranged on the outside of the sleeve 34 and a drive element 42 arranged on the outside of the sleeve 40. In further variants, the drive element 42 can be arranged directly on the sleeve 34.

Das Antriebselement 42 weist eine Außenumfangsfläche 44 auf, die für eine kraft- und/oder formschlüssige Wirkverbindung mit einem entsprechend ausgestalteten Abtriebselement 46, das weiter unten beschrieben wird, ausgestaltet ist.The drive element 42 has an outer circumferential surface 44 which is designed for a non-positive and / or positive connection with a correspondingly designed output element 46, which is described below.

Die Außenumfangsfläche 44 kann eine gezahnt Fläche aufweisen, um Kräfte und/oder Bewegungen eines ebenfalls eine Zahnung aufweisenden Abtriebselements 46 von diesem auf das Antriebselement 42 zu übertragen, um das Antriebselement 42 zu bewegen (z.B. rotatorisch und/oder translatorisch). In solchen Fällen kann das Antriebselement 42 auch als Zahnrad bezeichnet werden.The outer circumferential surface 44 can have a toothed surface in order to transmit forces and / or movements of an output element 46, which also has a toothing, from the latter to the drive element 42 in order to move the drive element 42 (e.g. rotationally and / or translationally). In such cases, the drive element 42 can also be referred to as a toothed wheel.

Die Außenumfangsfläche 44 kann eine Fläche haben, die z.B. durch Beschichtung und/oder aufgrund ihres Materials geeignet ist, sodass Kräfte und/oder Bewegungen eines Abtriebselements 46 auf das Antriebselement 42 und damit die Antriebsvorrichtung 38 durch Kraftschluss übertragen werden können, um diese zu bewegen (z.B. rotatorisch und/oder translatorisch). In solchen Fällen kann das Antriebselement 42 auch als Reibrad bezeichnet werden. In allen Fällen ist es vorgesehen, dass Bewegungen der Antriebsvorrichtung 38 auf die Hülse 34 und ein darin angeordneter Aerosol-Dosierbehälter 32 übertragen werden können.The outer circumferential surface 44 can have a surface that is suitable, for example, by coating and / or because of its material, so that forces and / or movements of an output element 46 can be transmitted to the drive element 42 and thus the drive device 38 by frictional engagement in order to move it ( eg rotational and / or translational). In such cases, the drive element 42 can also be referred to as a friction wheel. In all cases, it is provided that movements of the drive device 38 can be transmitted to the sleeve 34 and an aerosol dosing container 32 arranged therein.

Gegenüber einem Halterungshauptkörper 48 der Dosierbehälter-Halterung 30 ist die Antriebsvorrichtung 38 und damit die Hülse 34 sowie ein darin angeordneter Aerosol-Dosierbehälter 32 mittels einer Einrichtung 50 abgestützt und bewegbar gelagert. Die Einrichtung 50 kann, wie gezeigt, ein oder mehrere Kugellager 50 aufweisen, deren Außenring mit dem Halterungshauptkörper 48 und deren Innenring mit der Hülsen 34 und/oder der Hülse 40 verbunden sind.Compared to a holder main body 48 of the dosing container holder 30, the drive device 38 and thus the sleeve 34 and an aerosol dosing container 32 arranged therein are supported and movable by means of a device 50. As shown, the device 50 can have one or more ball bearings 50, the outer ring of which are connected to the main holder body 48 and the inner ring of which is connected to the sleeves 34 and / or the sleeve 40.

Die Aerosol-Dosieranordnung 2 umfasst ferner eine Antriebsvorrichtung 52, die ein zur Wirkverbindung mit der Antriebsvorrichtung 38 vorgesehenes Abtriebselement 46 aufweist und zum Antrieb der Antriebsvorrichtung 38 dient.The aerosol metering arrangement 2 further comprises a drive device 52 which has an output element 46 which is provided for the operative connection with the drive device 38 and which serves to drive the drive device 38.

Das Abtriebselement 46 weist eine Außenumfangsfläche 54 auf, die für eine kraft- und/oder formschlüssige Wirkverbindung mit einem entsprechend ausgestalteten Antriebselement 42 (s.o.) ausgestaltet ist.The output element 46 has an outer peripheral surface 54 which is designed for a non-positive and / or positive connection with a correspondingly configured drive element 42 (see above).

Die Außenumfangsfläche 42 kann eine gezahnt Fläche aufweisen. In solchen Fällen kann das Abtriebselement 46 auch als Zahnrad bezeichnet werden.The outer peripheral surface 42 may have a toothed surface. In such cases, the output element 46 can also be referred to as a toothed wheel.

Die Außenumfangsfläche 54 kann eine Fläche haben, die z.B. durch Beschichtung und/oder aufgrund ihres Materials geeignet ist, sodass Kräfte des Abtriebselements 46 auf ein Antriebselement 42 und damit ein Antriebsvorrichtung 38 durch Kraftschluss übertragen werden können, um diese zu bewegen (z.B. rotatorisch und/oder translatorisch). In solchen Fällen kann das Abtriebselement 46 auch als Reibrad bezeichnet werden.The outer peripheral surface 54 may have a surface that e.g. by coating and / or due to its material, so that forces of the output element 46 can be transmitted to a drive element 42 and thus a drive device 38 by frictional engagement in order to move them (e.g. rotationally and / or translationally). In such cases, the driven element 46 can also be referred to as a friction wheel.

Die Antriebsvorrichtung 52 umfasst einen steuerbaren Antrieb 56, beispielsweise einen Elektromotor, der vorzugsweise als Schrittmotor ausgeführt ist oder betrieben werden kann. Die Abtriebswelle 58 des Antriebes 56 kann direkt oder mittels einer Übersetzung/Getriebe mit dem Abtriebselement 46 verbunden sein, um Drehungen der Abtriebswelle 58 1:1 oder über-oder untersetzt auf das Abtriebselement 46 zu übertragen.The drive device 52 comprises a controllable drive 56, for example an electric motor, which is preferably designed or can be operated as a stepper motor. The output shaft 58 of the drive 56 can be connected directly or by means of a transmission / gearbox to the output element 46 in order to transmit rotations of the output shaft 58 1: 1 or step-down or reduced to the output element 46.

Der Antrieb 56 ist über eine drahtgebundene und/oder drahtlose Verbindung V mit einer Steuerung ST verbunden. Die Verbindung V kann auch dazu ausgelegt sein, den Antrieb 56 mit Energie zu versorgen. Alternativ kann eine separate Energieversorgung oder eine Energieversorgung vorgesehen sein, die in oder an der Aerosol-Dosieranordnung 2 angeordnet ist.The drive 56 is connected to a controller ST via a wired and / or wireless connection V. The connection V can also be designed to supply the drive 56 with energy. Alternatively, a separate energy supply or an energy supply can be provided, which is arranged in or on the aerosol metering arrangement 2.

Bei den obigen Varianten der Antriebsvorrichtungen 38 und 52 können Kräfte und/oder Bewegungen auf einen Aerosol-Dosierbehälter 32 übertragen werden, um diesen bezüglich seiner darstellungsgemäßen Längsachse zu drehen.In the above variants of the drive devices 38 and 52, forces and / or movements can be transmitted to an aerosol dosing container 32 in order to rotate it with respect to its longitudinal axis as shown.

Bei weiteren Varianten können die Antriebsvorrichtungen 38 und 52 ausgestaltet sein, um einen Aerosol-Dosierbehälter 32 in einer Richtung im Wesentlichen parallel zu seiner darstellungsgemäßen Längsachse translatorisch zu bewegen.In further variants, the drive devices 38 and 52 can be designed to translate an aerosol dosing container 32 in one direction essentially parallel to its longitudinal axis as shown.

Hierfür kann das Antriebselement 42 z.B. als sich in einer Richtung parallel zu darstellungsgemäßen Längsachse der Hülse 34 bzw. eines darin angeordneten Aerosol-Dosierbehälters 32 erstreckendes, längliches Antriebselement ausgebildet sein. Beispielsweise kann hierfür eine an der Außenseite der Hülse 34 (oder der Hülse 40) sich in deren Längsrichtung erstreckende längliche Zahnstange und/oder Reibschlussfläche und/oder -beschichtung verwendet werden.For this purpose, the drive element 42 can e.g. be designed as an elongated drive element extending in a direction parallel to the longitudinal axis of the sleeve 34 or an aerosol dosing container 32 arranged therein. For example, an elongated toothed rack and / or friction surface and / or coating on the outside of the sleeve 34 (or the sleeve 40) extending in the longitudinal direction thereof can be used.

Als Antriebselement 46 kann hier ebenfalls ein Zahnrad bzw. Reibrad verwendet werden, welches mittels des gesteuerten Antriebs 56 gesteuert betrieben werden kann, um einen Aerosol-Dosierbehälter 32 in gewünschter Weise translatorisch (darstellungsgemäß nach oben und/oder unten) zu bewegen.A gearwheel or friction wheel can also be used here as the drive element 46, which can be operated in a controlled manner by means of the controlled drive 56 in order to move an aerosol dosing container 32 in a desired manner in a translatory manner (upward and / or downward as shown).

Bei weiteren Varianten kann für mehrere Dosierbehälter-Halterungen 30 bzw. deren Antriebsvorrichtungen 38 eine gemeinsame Antriebsvorrichtung 52 verwendet werden, die z.B. einen gemeinsam genutzten Antrieb 56 und für die verschiedenen Dosierbehälter-Halterungen 30 bzw. deren Antriebsvorrichtungen 38 jeweils ein Abtriebselement 46 aufweist.In further variants, a common drive device 52 can be used for a plurality of metering container holders 30 or their drive devices 38, which e.g. has a shared drive 56 and for the various dosing container holders 30 or their drive devices 38 each has an output element 46.

Die Aerosol-Dosieranordnung 2 weist eine Betätigungsvorrichtung 60 auf, die ein selektiv steuerbares, bewegbares Betätigungselement 62 umfasst. Das Betätigungselement 62 kann beispielsweise mittels eines elektrischen oder hydraulischen Antriebs oder, wie gezeigt, über Pneumatikanschlüsse 64 und 66 pneumatisch bewegt werden.The aerosol metering arrangement 2 has an actuating device 60 which comprises a selectively controllable, movable actuating element 62. The actuating element 62 can be moved pneumatically, for example by means of an electric or hydraulic drive or, as shown, via pneumatic connections 64 and 66.

Das Betätigungselement 62 kann in Richtung des Pfeiles 68 auf einen Aerosol-Dosierbehälter 32 zu bewegt werden, um diesen zu kontaktieren und dadurch, wie weiter unten erläutert, zu betätigen. Durch Bewegung des Betätigungselements 62 entgegen der Richtung des Pfeiles 68 kann die Wirkverbindung mit dem Aerosol-Dosierbehälter 32 und damit dessen Betätigung beendet werden.The actuating element 62 can be moved in the direction of the arrow 68 towards an aerosol dosing container 32 in order to contact it and thereby actuate it, as explained further below. By moving the actuating element 62 against the direction of arrow 68, the operative connection with the aerosol dosing container 32 and thus its actuation can be terminated.

Die Aerosol-Dosieranordnung 2 kann einen mittels eines Gelenk-/Schwenkmechanismus 70 belegbaren Deckel 72 aufweisen. Wenn der Deckel 72 geöffnet ist (siehe Fig. 2a und 2b) können Aerosol-Behälter 32 in Dosierbehälter-Halterungen 30 eingebracht bzw. von dort wieder entfernt werden. Wenn der Deckel 72 geschlossen ist (siehe Fig. 1a und 1b) können in den Dosierbehälter-Halterungen 30 angeordnete Aerosol-Dosierbehälter 32 mittels der Betätigungsvorrichtung 60 betätigt werden und mittels der Antriebsvorrichtungen 38 und 52 bewegt werden.The aerosol metering arrangement 2 can have a cover 72 which can be assigned by means of a joint / swivel mechanism 70. When the lid 72 is open (see 2a and 2b ), aerosol containers 32 can be introduced into metering container holders 30 or removed therefrom. When the lid 72 is closed (see 1a and 1b ) Aerosol dosing containers 32 arranged in the dosing container holders 30 can be operated by means of the actuating device 60 and can be moved by means of the drive devices 38 and 52.

Die Aerosol-Dosieranordnung 2 kann eine Heiz- und/oder Kühlvorrichtung 74 umfassen, die beispielsweise Kanäle 76 aufweisen kann, durch die erwärmtes und/oder gekühltes Fluid (z.B. erwärmtes/gekühltes Wasser, Luft) geführt werden kann.The aerosol metering arrangement 2 can comprise a heating and / or cooling device 74, which can have channels 76, for example, through which heated and / or cooled fluid (e.g. heated / cooled water, air) can be guided.

Die Aerosol-Dosieranordnung 2 kann eine weitere Heiz- und/oder Kühlvorrichtung umfassen, die beispielsweise in die Dosierbehälter-Halterung 30 (dort z.B. in/an der Hülse 34 angeordnet) integriert ist, um einen dort angeordneten Aerosol-Dosierbehälter 32 auf eine vorbestimmte Temperatur oder in einen bestimmten Temperaturbereich zu bringen bzw. zu halten.The aerosol metering arrangement 2 can comprise a further heating and / or cooling device, which is integrated, for example, in the metering container holder 30 (there, for example, arranged in / on the sleeve 34) to an aerosol metering container 32 arranged there to a predetermined temperature or to bring or keep it in a certain temperature range.

Die Aerosol-Dosieranordnung 2 kann eine Fluidzufuhrvorrichtung 78 umfassen. Die Fluidzufuhrvorrichtung 78 kann dazu dienen Fluid, insbesondere Luft oder ein anderes Gas in durch einen Sedimentationskanal 16 strömendes und/oder darin absinkendes Aerosol einzubringen.The aerosol metering arrangement 2 can comprise a fluid supply device 78. The fluid supply device 78 can serve to introduce fluid, in particular air or another gas, into aerosol flowing through and / or sinking through a sedimentation channel 16.

Die Fluidzufuhrvorrichtung 78 weist einen oder mehrere Zufuhrleitungen 80 auf, die mit einer vorzugsweise steuerbaren Fluidquelle 82 verbunden werden können, die gewünschtes Fluid zuführen kann und beispielsweise eine Pumpe umfasst. Die Fluidquelle 82 kann auch gemeinsam für mehrere Sedimentationsrohre 14 verwendet werden.The fluid supply device 78 has one or more supply lines 80 which can be connected to a preferably controllable fluid source 82 which can supply the desired fluid and comprises, for example, a pump. The fluid source 82 can also be used together for several sedimentation tubes 14.

Darstellungsgemäß weist eine Fluidzufuhrvorrichtung 78 ferner Einleitkanäle 84 auf, die sich ausgehend von einer entsprechenden der Zufuhrleitungen 80 hin zu einem Sedimentationsrohr 14 erstrecken. Ebenfalls darstellungsgemäß weisen die Sedimentationsrohre 14 benachbart zum jeweiligen Sedimentationskanal-Einlass 20 Öffnungen 86 auf. Die freien Enden der Einleitkanäle 84, wo in Sedimentationskanäle 16 einzubringendes Fluid austreten kann, können in die Öffnungen 86 hineinragen oder sind von den Öffnungen 86 wenigstens so gering beabstandet, dass Fluid in die Öffnungen 86 und damit in Sedimentationskanäle 16 eingebracht werden kann. Derartige Varianten ermöglichen es, die Aerosol-Dosieranordnung 2 unterschiedlichen Typen von Expositionsmodulen zu verwenden. Bei weiteren Varianten können die Auslässen der Einleitkanäle 84 und/oder die Sedimentationsrohre 14 so ausgestaltet sein, dass Fluid direkt in die Sedimentationskanal-Einlässe 20 eingebracht werden können. Bei weiteren Varianten können die Einleitkanäle 84 an die Öffnungen 86 angeschlossen bzw. mit diesen verbunden sein.As shown, a fluid supply device 78 also has inlet channels 84, which extend from a corresponding one of the supply lines 80 to a sedimentation tube 14. Likewise, according to the illustration, the sedimentation tubes 14 have openings 86 adjacent to the respective sedimentation channel inlet 20. The free ends of the inlet channels 84, where fluid to be introduced into sedimentation channels 16 can escape, can protrude into the openings 86 or are at least slightly spaced from the openings 86 that fluid can be introduced into the openings 86 and thus into sedimentation channels 16. Such variants make it possible to use the aerosol metering arrangement 2 different types of exposure modules. In further variants, the outlets of the inlet channels 84 and / or the sedimentation tubes 14 can be configured such that fluid can be introduced directly into the sedimentation channel inlets 20. At In further variants, the inlet channels 84 can be connected to the openings 86 or connected to them.

Aerosol-DosierbehälterAerosol dosing container

Ein Aerosol-Dosierbehälter 32 umfasst einen Aerosol-Behälter 88, der einen Aerosol-Aufnahmeraum 90 definiert und eine Aerosol-Behälter-Öffnung 92 aufweist. Der Aerosol-Behälter 88 ist mittels eines an der Aerosol-Behälter-Öffnung 92 angebrachten Behälter-Verschlusses 94 fluiddicht verschlossen.An aerosol dosing container 32 comprises an aerosol container 88, which defines an aerosol receiving space 90 and has an aerosol container opening 92. The aerosol container 88 is closed in a fluid-tight manner by means of a container closure 94 attached to the aerosol container opening 92.

Der Behälter-Verschluss 94 umfasst ein Dosierventil 96, welches bei Betätigung eine vorbestimmte Menge an im Aerosol-Behälter 88 enthaltenen Aerosols über einen bewegbaren Ventilauslass 98 abgibt bzw. durchlässt.The container closure 94 comprises a metering valve 96 which, when actuated, releases or transmits a predetermined amount of aerosol contained in the aerosol container 88 via a movable valve outlet 98.

Das Dosierventil 96 kann so ausgestaltet sein, dass es durch eine Betätigung geöffnet wird und sich nach einer vorbestimmten Zeitdauer selbsttätig, automatisch schließt. Alternativ kann das Dosierventil 96 kann so ausgestaltet sein, dass es durch eine Betätigung geöffnet wird, solange geöffnet bleibt, solange die Betätigung andauert und sich erst schließt, wenn die Betätigung beendet wird.The metering valve 96 can be designed in such a way that it is opened by an actuation and closes automatically after a predetermined period of time. Alternatively, the metering valve 96 can be designed in such a way that it is opened by an actuation as long as it remains open, as long as the actuation continues and only closes when the actuation is ended.

Insbesondere ist ein Dosierventil 96 vorgesehen, das betätigt werden kann, indem für eine Relativbewegung zwischen Dosierventil 96 (und insbesondere dessen Ventilauslass 98) und Aerosol-Behälter 88 gesorgt wird.In particular, a metering valve 96 is provided which can be actuated by ensuring a relative movement between metering valve 96 (and in particular its valve outlet 98) and aerosol container 88.

Dies kann mittels der Betätigungsvorrichtung 60 erreicht werden, wenn deren Betätigungselement 62 auf den Boden 100 des Aerosol-Behälters 88 zubewegt wird und diesen kontaktiert und dadurch Kraft auf den Aerosol-Dosierbehälter 32 ausübt. Dies hat zur Folge, dass der Aerosol-Behälter 88 darstellungsgemäß nach unten bewegt wird. Dabei kontaktiert der Ventilauslass 98 des Dosierventils 96 das Ende eines Sedimentationsrohrs 14 mit dem Sedimentationskanal-Einlass 20 (oder ein anderes Element, das als Anschlag dient), wodurch der Ventilauslass 98 nicht (mehr) der Bewegung des Aerosol-Behälters 88 folgt und sich das Dosierventil 96 öffnet. Durch Steuerung der Bewegung und Positionierung des Betätigungselements 62 kann die Dauer der Öffnung des Dosierventils 96 gesteuert werden.This can be achieved by means of the actuating device 60 when its actuating element 62 is moved towards the bottom 100 of the aerosol container 88 and contacts it, thereby exerting force on the aerosol dosing container 32. As a result, the aerosol container 88 is moved downward as shown. The valve outlet 98 of the metering valve 96 contacts the end of a sedimentation tube 14 with the sedimentation channel inlet 20 (or another element which serves as a stop), as a result of which the valve outlet 98 no longer follows the movement of the aerosol container 88 and this occurs Dosing valve 96 opens. The duration of the opening of the metering valve 96 can be controlled by controlling the movement and positioning of the actuating element 62.

Aerosol in dem Aerosol-Dosierbehälter 32 kann Partikel und Gas, insbesondere Treibgas umfassen. Bei der Lagerung und auch bei Anordnung in einer Dosierbehälter-Halterung 30 kann es im Aerosol-Dosierbehälter 32 zu Sedimentation von im Aerosol enthaltenen Partikeln kommen. Dies führt dazu, dass das Aerosol im Aerosol-Dosierbehälter 32 keine homogene gleichmäßige Zusammensetzung (mehr) hat.Aerosol in the aerosol dosing container 32 can comprise particles and gas, in particular propellant gas. During storage and also when arranged in a dosing container holder 30, sedimentation of particles contained in the aerosol can occur in the aerosol dosing container 32 come. This means that the aerosol in the aerosol dosing container 32 does not have a homogeneous, uniform composition (anymore).

Um dies zu vermeiden bzw. die Folgen von Sedimentation im Aerosol-Dosierbehälter 32 zu beseitigen, sind im Aerosol-Behälter 88 ein oder mehrere Rührelemente 102 vorgesehen.In order to avoid this or to eliminate the consequences of sedimentation in the aerosol dosing container 32, one or more stirring elements 102 are provided in the aerosol container 88.

Ein oder mehrere Rührelemente 102 können an der Innenseite des Behälter-Verschlusses 94 angeordnet sein und sich ausgehend von diesem in den Aerosol-Aufnahmeraum 90 hinein erstrecken; vorzugsweise in Längsrichtung des Aerosol-Behälters 88 und/oder in einem geringen Winkel dazu. Es können beispielsweise drei Rührelemente gleicher oder unterschiedlicher Länge, auch mit abgeschrägten Enden verwenden werden. (siehe Fig. 9-12).One or more stirring elements 102 can be arranged on the inside of the container closure 94 and extend from this into the aerosol receiving space 90; preferably in the longitudinal direction of the aerosol container 88 and / or at a slight angle thereto. For example, three stirring elements of the same or different lengths, also with bevelled ends, can be used. (please refer Fig. 9-12 ).

Ergänzend oder alternativ können auch ein oder mehrere Rührelemente 102 vorgesehen sein, die sich ausgehend von der Innenseite am Boden 100 des Dosier-Behälters 88 nach innen in den Aerosol-Aufnahmeraum 90 hinein erstrecken; vorzugsweise in Längsrichtung des Aerosol-Behälters 88 und/oder in einem geringen Winkel dazu. Auch hier können beispielsweise drei Rührelemente gleicher oder unterschiedlicher Länge, auch mit abgeschrägten Enden verwenden werden, (siehe Fig. 13).Additionally or alternatively, one or more stirring elements 102 can also be provided, which, starting from the inside on the bottom 100 of the dosing container 88, extend inwards into the aerosol receiving space 90; preferably in the longitudinal direction of the aerosol container 88 and / or at a slight angle thereto. Here, too, three stirring elements of the same or different lengths, also with bevelled ends, can be used, for example (see Fig. 13 ).

Ergänzend oder alternativ können auch ein oder mehrere Rührelemente 102 vorgesehen sein, die sich ausgehend von einer inneren Mantelfläche 104 des Dosier-Behälters 88 nach innen in den Aerosol-Aufnahmeraum 90 hinein erstrecken; vorzugsweise quer zur Längsrichtung des Aerosol-Behälters 88 und/oder in einem geringen Winkel dazu. Auch hier können beispielsweise drei Rührelemente gleicher oder unterschiedlicher Länge, auch mit abgeschrägten Enden verwenden werden. (siehe Fig. 14)Additionally or alternatively, one or more stirring elements 102 can also be provided, which, starting from an inner lateral surface 104 of the dosing container 88, extend inwards into the aerosol receiving space 90; preferably transverse to the longitudinal direction of the aerosol container 88 and / or at a slight angle thereto. Here too, for example, three stirring elements of the same or different lengths can be used, even with bevelled ends. (please refer Fig. 14 )

Ergänzend oder alternativ können auch ein oder mehrere Rührelemente 102, die sich frei im Aerosol-Aufnahmeraum 90 bewegen können (s. Fig. 15-16). Solche Rührelemente 102 können teilweise magnetisch sein.Additionally or alternatively, one or more stirring elements 102, which can move freely in the aerosol receiving space 90 (see FIG. Fig. 15-16 ). Such stirring elements 102 can be partially magnetic.

Nicht freibewegliche Rührelemente 102 können an der Innenseite des Behälter-Verschlusses 94 und/oder der Innenseite des Bodens 100 und/oder der inneren Mantelfläche 104 des Dosier-Behälters 88 angebracht sein.Stirrer elements 102 that are not freely movable can be attached to the inside of the container closure 94 and / or the inside of the bottom 100 and / or the inner lateral surface 104 of the dosing container 88.

Alternativ oder ergänzend können nicht freibewegliche Rührelemente 102 an einem Einsatz E angebracht und/oder daran ausgebildet sein, der in den Dosier-Behälter 88 eingebracht werden kann.Alternatively or in addition, stirring elements 102 which are not freely movable can be attached and / or formed on an insert E which can be introduced into the dosing container 88.

Alternativ oder ergänzend können nicht freibewegliche Rührelemente 102 durch entsprechende Ausformungen der Innenseiten des Dosier-Behälters 88 bereitgestellt sein.As an alternative or in addition, stirring elements 102 which are not freely movable can be provided by correspondingly shaping the inside of the dosing container 88.

Die Funktion der Rührelemente 102 besteht darin, im Aerosol zu rühren bzw. dessen Bestandteile zu vermischen, um insbesondere nach Sedimentation für eine homogene Mischung/Verteilung von Aerosolbestandteilen zu sorgen.The function of the stirring elements 102 is to stir in the aerosol or to mix its components in order to ensure a homogeneous mixing / distribution of aerosol components, particularly after sedimentation.

Das Mischen mittels der Rührelemente 102 kann erreicht werden, indem diese relativ zum im Aerosol-Behälter 88 enthaltenen Aerosol bewegt werden.Mixing by means of the stirring elements 102 can be achieved by moving them relative to the aerosol contained in the aerosol container 88.

Dies kann dadurch erreicht werden, dass ein Aerosol-Dosierbehälter 32 insgesamt und damit auch mit diesem verbundene Rührelemente 102 bewegt werden, wodurch aufgrund der Trägheit des Aerosols bzw. dessen Bestandteilen im Aerosol-Behälter 88 eine Relativbewegung zu den Rührelementen 102 entsteht.This can be achieved in that an aerosol dosing container 32 as a whole and thus also the stirring elements 102 connected to it are moved, which results in a relative movement to the stirring elements 102 due to the inertia of the aerosol or its components in the aerosol container 88.

Eine solche Bewegung eines Aerosol-Dosierbehälters 32 kann erreicht werden, indem man den Aerosol-Dosierbehälter 32 manuell bewegt.Such movement of an aerosol dosing container 32 can be achieved by manually moving the aerosol dosing container 32.

Wenn der Aerosol-Dosierbehälter 32 in einer Dosierbehälter-Halterung 30 angeordnet ist, kann die Bewegung mittels der Antriebsvorrichtungen 38 und 52 erreicht werden.If the aerosol dosing container 32 is arranged in a dosing container holder 30, the movement can be achieved by means of the drive devices 38 and 52.

Ergänzend oder alternativ kann das Mischen mittels der Rührelemente 102 erreicht werden, wenn im Aerosol-Aufnahmeraum frei bewegliche Rührelemente 102 vorhanden sind. Bei Bewegung eines Aerosol-Dosierbehälters 32 (z.B. manuell oder mittels der Antriebsvorrichtungen 38 und 52) insgesamt werden sich die frei beweglichen Rührelemente 102 (wenigsten anfänglich) nicht mitbewegen, was zu einer Relativbewegung zum Aerosol im Aerosol-Behälter 88 bzw. dessen Bestandteilen führt.Additionally or alternatively, the mixing can be achieved by means of the stirring elements 102 if freely moving stirring elements 102 are present in the aerosol receiving space. When an aerosol dosing container 32 is moved (e.g. manually or by means of the drive devices 38 and 52), the freely moving stirring elements 102 (at least initially) will not move, which leads to a relative movement to the aerosol in the aerosol container 88 or its components.

Ergänzend oder alternativ können Rührelemente 102 verwendet werden, die teilweise magnetisch sind. Solche magnetischere Elemente 102 können mittels eines von außen angelegten Magnetfeldes bewegt werden, um, wie bei einem Magnetrührer, im Aerosol zu rühren bzw. dessen Bestandteile zu vermischen. Dies kann bei Aerosol-Dosierbehälter 32 an sich erfolgen, wenn an einen Aerosol-Dosierbehälter 32 entsprechendes Magnetfeld angelegt wird.Additionally or alternatively, stirring elements 102 can be used, which are partially magnetic. Such more magnetic elements 102 can be moved by means of an externally applied magnetic field in order to stir in the aerosol or to mix its components, as in the case of a magnetic stirrer. With aerosol dosing container 32 this can be done per se if a corresponding magnetic field is applied to an aerosol dosing container 32.

Allerdings ist es auch vorgesehen, dies bei einem in der Aerosol-Dosieranordnung 2 angeordneten Aerosol-Dosierbehälter 32 zu ermöglichen. Für solche Varianten umfasst die Aerosol-Dosieranordnung 2 eine Magnetfelderzeugungseinrichtung, die ein Magnetfeld erzeugen kann, um magnetische Rührelemente 102 in einem Aerosol-Aufnahmeraum zu bewegen.However, it is also envisaged to make this possible in the case of an aerosol dosing container 32 arranged in the aerosol dosing arrangement 2. For such variants, the aerosol metering arrangement includes 2 shows a magnetic field generating device which can generate a magnetic field in order to move magnetic stirring elements 102 in an aerosol receiving space.

Beispielhafte VerfahrensdurchführungExemplary procedure implementation

Im Expositionsmodul 4 sind Proben 10 angeordnet und das Expositionsmodul 4 ist wie in den Zeichnungen veranschaulicht unter der Aerosol-Dosieranordnung positioniert.Samples 10 are arranged in the exposure module 4 and the exposure module 4 is positioned below the aerosol metering arrangement, as illustrated in the drawings.

Zum Einbringen von Aerosol-Dosierbehälter 32 in Dosierbehälter-Halterungen 30 der Aerosol-Dosieranordnung 2 wird deren Deckel 72 geöffnet, wodurch die Aerosol-Dosierbehälter 32 in Behälter-Halterungen 30 eingebracht und dort sicher gehalten werden können. Danach wird der Deckel 72 geschlossen. Dies positioniert die Betätigungsvorrichtungen 60 bezüglich der Aerosol-Dosierbehälter 32 so, dass mittels der entsprechenden Betätigungselemente 62, wie oben beschrieben, die jeweiligen Dosierventile 96 betätigt werden können.For the introduction of aerosol dosing containers 32 into dosing container holders 30 of the aerosol dosing arrangement 2, the cover 72 thereof is opened, whereby the aerosol dosing containers 32 can be inserted into container holders 30 and held there securely. The lid 72 is then closed. This positions the actuating devices 60 with respect to the aerosol dosing container 32 such that the respective dosing valves 96 can be actuated by means of the corresponding actuating elements 62, as described above.

Um die Aerosol-Dosierbehälter 32, wie oben beschrieben, zu drehen, um mittels in diesen vorhandenen Rührelementen 102 Bestandteile von in den jeweiligen Aerosol-Behälter 88 enthaltenem Aerosols zu vermischen, wird die Antriebsvorrichtung 52 so gesteuert betrieben, dass die Aerosol-Dosierbehälter 32 in gewünschter Weise gedreht werden. Hierbei ist es möglich, gesteuert vorzugeben, wie lange, wie oft, wie schnell und/oder in welche Richtung die Drehung jeweils erfolgen soll. Dies kann für die verschiedenen Aerosol-Dosierbehälter 32 gemeinsam oder individuell vorgegeben werden.In order to rotate the aerosol dosing containers 32, as described above, in order to mix components of aerosols contained in the respective aerosol container 88 by means of stirring elements 102 present in them, the drive device 52 is operated in a controlled manner in such a way that the aerosol dosing containers 32 in be rotated as desired. It is possible to specify in a controlled manner how long, how often, how fast and / or in which direction the rotation should take place. This can be specified jointly or individually for the different aerosol dosing containers 32.

Ein solcher Mischvorgang kann vor jeder Abgabe von Aerosol aus den Aerosol-Dosierbehältern 32 durch Betätigung des jeweiligen Dosierventils 96, auch vor der erstmaligen, erfolgen.Such a mixing process can take place before each delivery of aerosol from the aerosol metering containers 32 by actuating the respective metering valve 96, even before the first one.

Die Mischvorgänge bezüglich der verschiedenen Aerosol-Dosierbehälter 32 können gleichzeitig, zeitversetzt, überlappend etc. erfolgen.The mixing processes with respect to the different aerosol dosing containers 32 can take place simultaneously, with a time delay, overlapping etc.

Um einzelne, mehrere oder alle Proben mit Aerosol zu beaufschlagen wird bzw. werden die entsprechenden Aerosol-Dosierbehälter 32, wie oben beschrieben, mittel der jeweiligen Betätigungsvorrichtung 60 betätigt. Hierbei ist es möglich, gesteuert vorzugeben, wie lange, wie oft, und/oder wie schnell eine Betätigung jeweils erfolgen soll. Dies kann für die verschiedenen Aerosol-Dosierbehälter 32 gemeinsam oder individuell vorgegeben werden.In order to apply aerosol to individual, several or all samples, the corresponding aerosol dosing containers 32 are actuated by means of the respective actuating device 60, as described above. It is possible to specify in a controlled manner how long, how often, and / or how quickly an operation should take place in each case. This can be specified jointly or individually for the different aerosol dosing containers 32.

In Folge der Betätigung gibt das jeweilige Dosierventil 96 eine bestimmte Menge an Aerosol ab, das über einen entsprechenden Sedimentationskanal zur jeweiligen Probe gelangt.As a result of the actuation, the respective metering valve 96 releases a certain amount of aerosol, which reaches the respective sample via a corresponding sedimentation channel.

Dabei, aber auch schon davor und danach, kann über die jeweilige (oder gegebenenfalls gemeinsame) Fluidzufuhrvorrichtung 78 Fluid (z.B. Luft) in den entsprechenden Sedimentationskanal eingebracht werden, um beispielsweise das Aerosol zu verdünnen.In the process, but also before and afterwards, fluid (e.g. air) can be introduced into the corresponding sedimentation channel via the respective (or possibly common) fluid supply device 78, for example in order to dilute the aerosol.

Dabei, aber auch schon davor und danach, kann über die jeweilige Absaugöffnung 22 Fluid (z.B. Luft, Treibgas) aus dem entsprechenden Sedimentationskanal entfernt werden, um beispielsweise unerwünschte Einwirkungen auf die jeweilige Probe 10 zu verhindern.During this, but also before and after, fluid (e.g. air, propellant gas) can be removed from the corresponding sedimentation channel via the respective suction opening 22, for example in order to prevent undesirable effects on the respective sample 10.

Die Aerosolabgabe kann einmal, mehrmals und solange erfolgen, bis nicht mehr Aerosol zur Verfügung steht bzw. nicht mehr in einer solchen Menge zur Verfügung steht, dass die vorbestimmte Aerosolmenge abgegeben werden kann. Zwischen jeder oder nach einer vorbestimmten Anzahl an Aerosolabgaben kann ein oder mehrere Mischvorgänge ("Drehen von Aerosol-Dosierbehältern") durchgeführt werden.The aerosol can be dispensed once, several times and until the aerosol is no longer available or is no longer available in such an amount that the predetermined amount of aerosol can be dispensed. One or more mixing processes (“turning aerosol dosing containers”) can be carried out between each or after a predetermined number of aerosol deliveries.

Zur Entnahme der Aerosol-Dosierbehälter 32 wird der Deckel 72 geöffnet, um die Aerosol-Dosierbehälter 32 zu entnehmen und gegebenenfalls durch andere zu ersetzen. BEZUGSZEICHENLISTE Aerosol-Dosieranordnung 2 Expositionsmodul 4 Probenaufnahmebereich 6 Insert 8 Probe 10 Nährlösung 12 Sedimentationsrohr 14 Sedimentationskanal 16 Sedimentationskanal-Auslass 18 Sedimentationskanal-Einlass 20 Absaugöffnung 22 Absaugleitung 24 Absaugvorrichtung 26 Absaugrohr 28 Dosierbehälter-Halterung 30 Aerosol- Dosierbehälter 32 Hülse zur Aufnahme eines Aerosol-Dosierbehälters 34 O-Ring 36 Antriebsvorrichtung (Halterung) 38 Hülse der Antriebsvorrichtung 40 Antriebselement 42 Außenumfangsfläche des Antriebselements 44 Abtriebselement 46 Halterungshauptkörper 48 Stütz-/Lager-Einrichtung (Kugellager) 50 Antriebsvorrichtung 52 Außenumfangsfläche des Abtriebselements 54 Antrieb der Antriebsvorrichtung 56 Verbindung Antrieb Steuerung V Steuerung ST Abtriebswelle des Antriebes 58 Betätigungsvorrichtung 60 Betätigungselement 62 Pneumatikanschluss 64 Pneumatikanschluss 66 Pfeil (Betätigungsrichtung des Betätigungselementes) 68 Gelenk-/Schwenkmechanismus 70 Deckel 72 Heiz- und/oder Kühlvorrichtung 74 Kanäle der Heiz- und/oder Kühlvorrichtung 76 Fluidzufuhrvorrichtung 78 Zufuhrleitungen 80 Fluidquelle 82 Einleitkanäle 84 Öffnungen an Sedimentationskanal-Einlass 86 Aerosol-Behälter 88 Aerosol-Aufnahmeraum 90 Aerosol-Behälter-Öffnung 92 Behälter-Verschluss 94 Dosierventil 96 Ventilauslass 98 Boden Aerosol-Dosierbehälter 100 Rührelement 102 innere Mantelfläche Aerosol-Dosierbehälter 104 Einsatz E To remove the aerosol dosing containers 32, the cover 72 is opened in order to remove the aerosol dosing containers 32 and, if necessary, to replace them with others. <b> REFERENCE SIGN LIST </b> Aerosol dosing arrangement 2nd Exposure module 4th Sampling area 6 Insert 8th sample 10th Nutrient solution 12th Sedimentation tube 14 Sedimentation channel 16 Sedimentation channel outlet 18th Sedimentation channel inlet 20 Suction opening 22 Suction line 24th Suction device 26 Exhaust pipe 28 Dosing container holder 30th Aerosol dosing container 32 Sleeve for holding an aerosol dosing container 34 O-ring 36 Drive device (bracket) 38 Drive device sleeve 40 Drive element 42 Outer peripheral surface of the drive element 44 Output element 46 Bracket main body 48 Support / bearing device (ball bearing) 50 Drive device 52 Outer peripheral surface of the output element 54 Drive the drive device 56 Connection drive control V control ST Output shaft of the drive 58 Actuator 60 Actuator 62 Pneumatic connection 64 Pneumatic connection 66 Arrow (direction of actuation of the actuating element) 68 Articulated / swivel mechanism 70 cover 72 Heating and / or cooling device 74 Channels of the heating and / or cooling device 76 Fluid supply device 78 Supply lines 80 Fluid source 82 Inlet channels 84 Openings at the sedimentation channel inlet 86 Aerosol container 88 Aerosol recording room 90 Aerosol container opening 92 Container closure 94 Dosing valve 96 Valve outlet 98 Bottom aerosol dosing container 100 Stirring element 102 inner surface of aerosol dosing container 104 commitment E

Claims (12)

  1. Aerosol dosing container (32) for metered delivery of aerosol, comprising:
    - an aerosol container (88) for receiving aerosol, said aerosol container defining an aerosol receiving space (90) and having an aerosol container opening (92),
    - a container closure (94) provided for the fluid-tight closure of the aerosol container opening (92), said container closure comprising an actuatable metering valve (96) for the metered delivery of aerosol contained in the aerosol receiving space (90),
    - at least one stirring element (102) extending in the aerosol receiving space and connected in a stationary manner to an inner side of the aerosol container (88) and/or the container closure (94),
    wherein a positive-locking and/or frictionally engaged drive element (42) is arranged on an outer side of the aerosol container (88) for moving the aerosol metering container (32) relative to the aerosol contained therein, so that, when the aerosol metering container (32) is moved, the at least one stirring element (102) moves relative to the aerosol contained in the aerosol receiving space in order to stir it.
  2. Aerosol metering container according to patent claim 1, wherein the at least one stirring element (102) has at least one stirring element (120) extending in a direction parallel and/or perpendicular to a longitudinal axis of the aerosol container (88).
  3. Aerosol dosing container according to patent claim 1 or 2, wherein
    - the at least one stirring element is arranged in the aerosol container (88) by means of an insert arranged in the aerosol receiving space.
  4. Aerosol dosing container according to one of the previous patent claims, further comprising a magnetic stirring fish movably arranged in the aerosol receiving space.
  5. Aerosol metering arrangement, comprising:
    - an aerosol metering container (32) according to one of the previous patent claims 1 to 4,
    - a dosing container holder (30) for arranging the aerosol dosing container (32) for the dosed delivery of aerosol by actuating the dosing valve (96) of the aerosol dosing container (32),
    - an actuating device (60) for actuating the dosing valve (96) of the aerosol dosing container (32),
    - a controllable movement device to move the aerosol dosing container (32) in a controlled manner.
  6. Aerosol dosing arrangement according to patent claim 5, wherein
    - the movement device comprises a positive and/or frictional drive element (42) provided for engagement with the positive and/or frictional drive element (46) of the aerosol metering container (32).
  7. Aerosol metering arrangement according to patent claim 5 or 6, further comprising a fluid supply device (78) for supplying fluid into aerosol dispensed from the aerosol metering container (32).
  8. Aerosol dosing system comprising:
    - an aerosol dosing arrangement (2) according to one of patent claims 6 to 8, and
    - an exposure module (4) with a sedimentation tube (14) for supplying aerosol dispensed by the aerosol dosing arrangement (2) to a sample (10), wherein
    - the sedimentation tube (14) has a suction opening (22) for sucking fluid out of the sedimentation tube (14).
  9. Method for mixing components of an aerosol in an aerosol dosing container, comprising:
    - providing an aerosol dosing container (32) in accordance with one of patent claims 1 to 4,
    - moving the aerosol dosing container (32) and thus the at least one stirring element relative to the aerosol to thereby mix its components.
  10. Method according to patent claim 9, wherein a predetermined amount of aerosol is dispensed from the aerosol dosing container.
  11. Method according to patent claim 10, wherein the step of dispensing a predetermined amount of aerosol from the aerosol metering container is carried out after or before the step of moving the at least one stirring element relative to the aerosol and these steps are repeated at least twice at predetermined time intervals.
  12. Method according to any one of patent claims 9 to 11, wherein
    - a predetermined amount of fluid is supplied into the aerosol dispensed from the aerosol dispensing container, and/or
    - a predetermined amount of fluid is removed from the aerosol dispensed from the aerosol dispensing container.
EP17001888.1A 2016-11-18 2017-11-18 Device and method for aerosol exposure Active EP3342486B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102016013773.8A DE102016013773B4 (en) 2016-11-18 2016-11-18 Devices and methods for aerosol exposure

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EP3342486A2 EP3342486A2 (en) 2018-07-04
EP3342486A3 EP3342486A3 (en) 2018-10-03
EP3342486B1 true EP3342486B1 (en) 2020-07-29

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Application Number Title Priority Date Filing Date
EP17001888.1A Active EP3342486B1 (en) 2016-11-18 2017-11-18 Device and method for aerosol exposure

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EP (1) EP3342486B1 (en)
DE (1) DE102016013773B4 (en)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5829648A (en) * 1997-01-21 1998-11-03 Bath & Body Works, Inc. Sheet spray and sprayer with beads
GB2341642A (en) * 1998-07-28 2000-03-22 Robert George Shephard Aerosol container with different sized agitating elements
GB9924808D0 (en) * 1999-10-21 1999-12-22 Glaxo Group Ltd Medicament dispenser
JP2005145457A (en) * 2003-10-20 2005-06-09 Mitani Valve Co Ltd Valve mechanism for aerosol container
GB2460620B (en) * 2008-03-07 2012-12-12 Otter Controls Ltd Electrical appliances and components

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

Also Published As

Publication number Publication date
DE102016013773B4 (en) 2023-09-07
EP3342486A3 (en) 2018-10-03
DE102016013773A1 (en) 2018-06-07
EP3342486A2 (en) 2018-07-04

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