EP2780479A4 - Quantitative, highly multiplexed detection of nucleic acids - Google Patents

Quantitative, highly multiplexed detection of nucleic acids

Info

Publication number
EP2780479A4
EP2780479A4 EP12849697.3A EP12849697A EP2780479A4 EP 2780479 A4 EP2780479 A4 EP 2780479A4 EP 12849697 A EP12849697 A EP 12849697A EP 2780479 A4 EP2780479 A4 EP 2780479A4
Authority
EP
European Patent Office
Prior art keywords
quantitative
nucleic acids
highly multiplexed
multiplexed detection
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP12849697.3A
Other languages
German (de)
French (fr)
Other versions
EP2780479A1 (en
Inventor
Kris Scaboo
Patrick Martin
Brad Taft
Jason La
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NVS TECHNOLOGIES Inc
Original Assignee
NVS TECHNOLOGIES Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/399,872 external-priority patent/US20120214686A1/en
Application filed by NVS TECHNOLOGIES Inc filed Critical NVS TECHNOLOGIES Inc
Publication of EP2780479A1 publication Critical patent/EP2780479A1/en
Publication of EP2780479A4 publication Critical patent/EP2780479A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP12849697.3A 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids Withdrawn EP2780479A4 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161561198P 2011-11-17 2011-11-17
US13/399,872 US20120214686A1 (en) 2011-02-18 2012-02-17 Quantitative, Highly Multiplexed Detection of Nucleic Acids
PCT/US2012/025699 WO2012112925A2 (en) 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids
PCT/US2012/051236 WO2013074163A1 (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids

Publications (2)

Publication Number Publication Date
EP2780479A1 EP2780479A1 (en) 2014-09-24
EP2780479A4 true EP2780479A4 (en) 2015-04-08

Family

ID=48430026

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12849697.3A Withdrawn EP2780479A4 (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids

Country Status (12)

Country Link
EP (1) EP2780479A4 (en)
JP (1) JP2014533502A (en)
KR (1) KR20140094007A (en)
CN (1) CN104093857A (en)
AU (1) AU2012337389A1 (en)
BR (1) BR112014011937A2 (en)
CA (1) CA2855953A1 (en)
HK (1) HK1199746A1 (en)
IL (1) IL232586A0 (en)
MX (1) MX338076B (en)
SG (1) SG11201402341QA (en)
WO (1) WO2013074163A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015008985A1 (en) * 2013-07-15 2015-01-22 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
EP3283501B1 (en) 2015-04-16 2023-01-04 William Marsh Rice University Stoichiometric tuning of nucleic acid hybridization probes by auxiliary oligonucleotide species
CN110016500A (en) * 2018-01-10 2019-07-16 深圳闪量科技有限公司 Surface-probe quantifying PCR method
WO2021102039A1 (en) * 2019-11-21 2021-05-27 10X Genomics, Inc, Spatial analysis of analytes
CN111808989A (en) * 2020-06-18 2020-10-23 重庆浦洛通基因医学研究院有限公司 Coronavirus/influenza virus/rhinovirus nucleic acid combined detection kit and use method thereof
KR20230085149A (en) 2020-10-15 2023-06-13 제넨테크, 인크. Multiplexed target-binding candidate screening analysis

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0601834A1 (en) * 1992-12-07 1994-06-15 Wisconsin Alumni Research Foundation Method of cleaving a nucleic acid molecule
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
US20080241838A1 (en) * 2006-12-29 2008-10-02 Applera Corporation, Applied Biosystems Group Methods and systems for detecting nucleic acids

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7189508B2 (en) * 2001-07-17 2007-03-13 Stratagene California Methods for detection of a target nucleic acid by capture using multi-subunit probes
WO2005010199A2 (en) * 2003-07-16 2005-02-03 Geneohm Sciences, Inc. Invasive cleavage reaction with electrochemical readout
AU2003300271A1 (en) * 2003-12-19 2005-08-03 Beckman Coulter, Inc. Solid-phase multiplexed invader assay
DK2038432T3 (en) * 2006-06-30 2017-04-03 Rosetta Genomics Ltd METHOD OF DETECTING AND QUANTIFYING A TARGET NUCLEIC ACID GENERATED BY RT-PCR BY MIRNA
EP2077336A1 (en) * 2007-12-19 2009-07-08 Koninklijke Philips Electronics N.V. Device and method for parallel quantitative analysis of multiple nucleic acids
CN102224257A (en) * 2008-11-21 2011-10-19 皇家飞利浦电子股份有限公司 Real time multiplex pcr detection on solid surfaces using double stranded nucleic acid specific dyes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0601834A1 (en) * 1992-12-07 1994-06-15 Wisconsin Alumni Research Foundation Method of cleaving a nucleic acid molecule
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
US20080241838A1 (en) * 2006-12-29 2008-10-02 Applera Corporation, Applied Biosystems Group Methods and systems for detecting nucleic acids

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BERNARD JUSKOWIAK: "Nucleic acid-based fluorescent probes and their analytical potential", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, SPRINGER, BERLIN, DE, vol. 399, no. 9, 29 October 2010 (2010-10-29), pages 3157 - 3176, XP019885328, ISSN: 1618-2650, DOI: 10.1007/S00216-010-4304-5 *
MATSUBARA Y ET AL: "Microchamber array based DNA quantification and specific sequence detection from a single copy via PCR in nanoliter volumes", BIOSENSORS AND BIOELECTRONICS, ELSEVIER BV, NL, vol. 20, no. 8, 15 February 2005 (2005-02-15), pages 1482 - 1490, XP027619503, ISSN: 0956-5663, [retrieved on 20050215] *
See also references of WO2013074163A1 *
SHALON D ET AL: "A DNA MICROARRAY SYSTEM FOR ANALYZING COMPLEX DNA SAMPLES USING TWO-COLOR FLUORESCENT PROBE HYBRIDIZATION", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, WOODBURY, NY, US, vol. 6, no. 7, 1 July 1996 (1996-07-01), pages 639 - 645, XP009007958, ISSN: 1088-9051 *

Also Published As

Publication number Publication date
SG11201402341QA (en) 2014-06-27
CA2855953A1 (en) 2013-05-23
WO2013074163A1 (en) 2013-05-23
HK1199746A1 (en) 2015-07-17
BR112014011937A2 (en) 2017-05-30
MX2014005912A (en) 2014-09-04
KR20140094007A (en) 2014-07-29
JP2014533502A (en) 2014-12-15
CN104093857A (en) 2014-10-08
AU2012337389A1 (en) 2014-06-19
IL232586A0 (en) 2014-06-30
EP2780479A1 (en) 2014-09-24
MX338076B (en) 2016-04-01

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