EP2536490A1 - Mischer zum einsetzen in einen rotor einer zentrifuge - Google Patents
Mischer zum einsetzen in einen rotor einer zentrifugeInfo
- Publication number
- EP2536490A1 EP2536490A1 EP11710456A EP11710456A EP2536490A1 EP 2536490 A1 EP2536490 A1 EP 2536490A1 EP 11710456 A EP11710456 A EP 11710456A EP 11710456 A EP11710456 A EP 11710456A EP 2536490 A1 EP2536490 A1 EP 2536490A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- rotor
- angular velocity
- mixing
- mixer
- mixing trough
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/05—Stirrers
- B01F27/051—Stirrers characterised by their elements, materials or mechanical properties
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F29/00—Mixers with rotating receptacles
- B01F29/15—Use of centrifuges for mixing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F29/00—Mixers with rotating receptacles
- B01F29/60—Mixers with rotating receptacles rotating about a horizontal or inclined axis, e.g. drum mixers
- B01F29/63—Mixers with rotating receptacles rotating about a horizontal or inclined axis, e.g. drum mixers with fixed bars, i.e. stationary, or fixed on the receptacle
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/50—Mixing receptacles
- B01F35/513—Flexible receptacles, e.g. bags supported by rigid containers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F29/00—Mixers with rotating receptacles
- B01F29/40—Parts or components, e.g. receptacles, feeding or discharging means
- B01F29/401—Receptacles, e.g. provided with liners
- B01F29/402—Receptacles, e.g. provided with liners characterised by the relative disposition or configuration of the interior of the receptacles
- B01F29/4022—Configuration of the interior
- B01F29/40222—Configuration of the interior provided with guide tubes on the wall or the bottom
Definitions
- Embodiments of the present invention relate to a mixer for insertion into a rotor of a centrifuge, for example a standard laboratory centrifuge.
- a mixing process can be carried out, for example, in a reaction vessel which is introduced into a centrifuge.
- a reaction vessel which is introduced into a centrifuge.
- two different liquids may be added to a reaction vessel, such as a glass tube or plastic tube. This is then added to mix the two liquids in a centrifuge and centrifuged.
- a disadvantage of the use of such standard reaction vessels for mixing liquids is that, due to the inertia of standard centrifuges, a mixing process, in particular in the case of liquids of different densities, does not take place or at least does not take place completely.
- Embodiments of the present invention provide a mixer for insertion into a rotor of a centrifuge.
- the mixer has a mixing trough and an obstacle device with at least one obstacle, which is designed to influence a flow of a liquid located in the mixing trough.
- a distance between at least one wall portion of the mixing trough and the obstacle device is variable. The liquid in the mixing tub flows around the obstacle of the obstacle device.
- Embodiments of the present invention thus enable a mixing of liquids based on a rotation of the rotor and thus on a centrifugal force generated by the rotor.
- the distance between the wall portion of the mixing trough and the obstacle device may vary in response to an angular velocity of the rotor of the centrifuge.
- embodiments of the present invention enable mixing of liquids based on the angular velocity of the rotor, wherein by varying the angular velocity of the rotor, one or more liquids can bypass the at least one obstacle multiple times to achieve a mixing effect.
- a mixer may include a return means.
- the return means is designed to generate a restoring force acting in the opposite direction to at least one component, a centrifugal force generated by the rotation of the rotor.
- the return means is designed such that in a first phase, at a first angular speed of the rotor, a first amount of the component of the centrifugal force acting in the opposite direction to the restoring force is greater than an amount of the restoring force. In a second phase, at a second angular velocity of the rotor, a second amount of the component of the centrifugal force acting in the opposite direction to the restoring force is smaller than the the restoring force.
- the amount of restoring force generated by the return means may be independent of the angular velocity of the rotor.
- a first distance of the wall section of the mixing trough to the obstacle device is greater than a second distance of the wall section of the mixing trough to the obstacle device in the second phase.
- a liquid present in the mixer or at least a part of the liquid flows around the at least one obstacle of the obstacle device in a first direction in the first phase.
- the liquid in the mixer or at least part of the liquid in the mixer flows around the at least one obstacle of the obstacle device in a direction opposite the first direction.
- the multiple flow around the liquid of the obstacle creates a mixing effect of the liquid in the mixer or of the liquid mixture present in the mixer.
- Embodiments of the present invention thus allow a mixture of different liquids based on an angular velocity of the rotor of a centrifuge.
- the return means may be formed as a spring.
- the wall portion of the obstruction means may be an elastic membrane and the elastic membrane may constitute the return means itself.
- the elastic membrane can act as a quasi pump, in the first phase, the elastic membrane is thereby stretched radially outwards (away from a rotational axis of the rotor) based on the centrifugal force and in the second phase, the elastic membrane is based on the through them generated restoring force radially inwardly (toward the axis of rotation of the rotor) and thus presses the liquid past the at least one obstacle of the obstacle device.
- the mixing trough may be movably mounted in the mixer, for example with respect to a housing of the mixer, wherein in the first phase, the mixing trough moves radially outward and in the second phase based on the restoring force generated by the spring moves radially inwardly to press the liquid past the at least one obstacle of the obstacle device.
- the liquid moves radially further inwards from a first location to a second location radially further outward.
- the liquid moves from the second location radially further outward to the first location radially further inward.
- the mixing trough can be firmly locked in the mixer, for example on a housing of the mixer.
- the obstacle device can be arranged to be movable in the mixing trough.
- the spring can be arranged, for example, between the wall portion of the mixing trough and the obstacle device. In the first phase, the obstacle device moves radially outward (based on the centrifugal force, as it were, through the liquid in the mixing trough), and in the second phase, the obstacle device moves radially inward based on the restoring force generated by the spring ,
- FIG. 1 is a schematic representation of a mixer according to an embodiment of the present invention.
- FIGS. 2a and 2b are schematic representations of embodiments according to the present invention.
- FIGS. 3a and 3b are schematic representations of further embodiments of the present invention.
- Fig. 4 is a schematic representation of another embodiment of the present invention.
- Fig. 5 is a schematic representation of another embodiment of the present invention
- Fig. 6 is a schematic representation of a device for receiving in a
- Fig. 1 shows a schematic representation of a mixer 10 according to an embodiment of the present invention.
- the mixer 10 for insertion into a rotor of a centrifuge has a mixing trough 11 and an obstacle device 12 with a first obstacle 9a and a second obstacle 9b. Between the first obstacle 9a and the second obstacle 9b, the wiper mixer 10 has a passage opening 13.
- an obstacle device may also have only one obstacle or a plurality of obstacles.
- An obstacle may be, for example, a bollard, a part of a rake (for example a ridge of a rake), a border or border of a through-opening (as shown by way of example in FIG. 1) or the like.
- a distance Li between a wall portion 14 of the mixing trough 1 1 and the obstacle device 12 is, in response to rotation of the rotor and at a proper recording of the mixer 10 in a holder of the rotor variable so that the liquid contained in the mixing trough 11 15 the obstacles 9a and 9b of the obstacle device 12 flows around.
- the liquid 15 flows through the passage opening 13 of the obstacle device 12 therethrough.
- the distance Li between the wall section 14 of the mixing trough 11 and the obstacle device 12 may be dependent on the angular velocity of the rotor of the centrifuge.
- a mixing of the liquid 15 located in the mixing tub 11 can thus be generated by changing the angular velocity of the rotor, wherein the liquid 15 thereby repeatedly through the at least one through hole 13 of the obstacle device 12 flows through (each in the opposite direction) and thereby several times the obstacles 9a, 9b of the obstacle device 12 flows around.
- the wall portion 14 of the mixing trough 1 1 form a bottom of the mixer 10 and can be arranged radially further outwardly than the obstacle device 12 during rotation of the mixer in the rotor of the centrifuge.
- the obstacle device 12 may be arranged in the mixing trough 11.
- the obstacle device 12 can be arranged movably in the mixing trough 1 1 or be locked in the mixing trough 1 1 (for example, at one edge of the mixing trough 1 1).
- the obstacle device 12 may be mechanically coupled to the mixing trough 11.
- Fig. 2a shows two mixers according to embodiments of the present invention.
- the mixer 20 shown above in FIG. 2 differs from the mixer 10 shown in FIG. 1 in that the obstacle device 12 has a plurality of passage openings 13 (five passage openings 13 are shown in FIG. 2 a above) and thus a plurality of obstacles 9 having.
- the schematic representation of the mixer 20 shown in FIG. 2 a above may, for example, be a sectional view of the mixer 20.
- the obstacle device 12 may therefore have further through openings 13 and obstacles 9, not shown here.
- the obstacles 9 can be designed so that the passage openings 13 are formed for example in the form of holes or strips.
- the mixer 20 has a housing 17, on which the obstacle device 12 is arranged.
- the mixing trough 11 is movably mounted in the housing 17 on a spring 16, which forms a return means.
- the spring 16 may be arranged, for example, between the wall portion 14 of the mixing trough 1 1 and a bottom (not shown here) of the housing 17.
- the variable distance between the wall portion 14, which may be a bottom of the mixing trough 1 1, for example, and the obstacle device 12 is realized in the mixer 20 shown in Fig. 2a above, that upon rotation of the mixer 20 about an axis of rotation 140 of the rotor the centrifuge, a generated by the rotation, centrifugal force F z opposite to a restoring force F r generated by the spring 16 acts.
- the mixing trough 1 1 moves radially outward and thus moves away from the obstacle device 12, whereby the distance Li between the wall section 14 and the obstacle device 12 enlarged.
- a liquid 15 located in the mixing trough 11 is pressed or flows through the passage openings 13 of the obstacle device 12 due to the centrifugal force.
- the liquid 15 thus flows from a radially inner location (from a location a smaller distance to the rotational axis 150 of the rotor) to a radially outer location (a greater distance from the rotational axis 140).
- the phase in which the centrifugal force F z is greater than the restoring force F r may be referred to as a first phase of the mixer 20.
- a phase in which the restoring force F r is greater than the centrifugal force F z may also be referred to as a second phase of the mixer 20.
- This rise and fall or movement of the mixing trough 11 from a radially inner location to a radially outer location can be carried out several times during a mixing operation, for example based on an alternating rotational frequency of the rotor of the centrifuge.
- the flow around the obstacle device 12 (the obstacles 9) and thus the flow through the liquid 15 through the through holes 13 of the Hindemis Rhein 12 are controlled.
- a flexible component (the mixing trough 11) moves relative to a fixed component (the hindrance device 12).
- a liquid (the liquid 15) is forced to flow around the solid component (the hindrance device 12 with the obstacles 9 and with the passage openings 13).
- the flexible component has been realized by a mixing trough 11 mounted on the spring 16.
- the centrifugal force causes a deflection of the flexible member (the mixing trough 11) from a radially further inward location to a radially outward location.
- a Force the restoring force F r generated by the spring, which counteracts the centrifugal force F z .
- a first arrow 18 in Fig. 2a above indicates a direction of centrifugal force Fz and an amount of centrifugal force F z .
- a second arrow 19 indicates a direction of the restoring force F r generated by the spring 16 and an amount of the restoring force F r .
- a length of the arrows 18, 19 represents a size of the amount of the respective force. From the length of the two arrows 18 and 19 in Fig. 2a above is thus apparent that an amount of the restoring force F r is greater than an amount of the centrifugal force F z .
- the mixer 20 is therefore located in the schematic representation of the mixer 20 shown in FIG. 2 a in the second phase already described above.
- Fig. 2a shows a mixer 21 according to another embodiment of the present invention.
- the mixer 21 differs from the mixer 20 shown in Fig. 2a above in that a wall portion 14 'whose distance L ⁇ to an obstacle device 12' is variable, is oblique.
- the distance L 2 from the wall portion 14 'to the axis of rotation 140 of the rotor at the right edge of the mixing trough 11 may be greater than at the left edge of the mixing trough 11.
- An embodiment of the wall portion 14', as shown in Fig. 2a, may in particular lead to a better mixing of liquids of different densities.
- the obstacle device 12 ' is arranged obliquely in the mixer 21. That is, a distance L 3 from a first passage opening 13a to the rotational axis 140 of the rotor of the centrifuge is different (in the embodiment shown in FIG. 2a greater) than a distance L 4 of a second passage opening 13b to the rotational axis 140 of the rotor , In other words, a first distance of a first obstacle 9a to the axis of rotation 140 of the rotor is different to a second distance of a second obstacle 9b to the axis of rotation 140 of the rotor.
- the obstacle device 12 ' may extend in a direction of propagation, for example from a right side of the obstacle device 12' to a left side of the obstacle device 12 'parallel to the wall portion 14' of the mixing trough 11.
- the passage openings 13 have different cross sections, that is to say, for example, opening diameters.
- an opening cross-section of the first through-opening 13a may be smaller than an opening cross-section of the second through-opening 13a. be opening 13b.
- a first distance between two obstacles of the obstacle device 12 ' is thus different from a second distance between two further obstacles of the obstacle device 12'.
- a mixer according to an exemplary embodiment of the present invention may also have only one inclined wall section 14 'or an inclined obstacle device 12' or different distances of the obstacles 9 from each other (and thus different cross sections of the through openings 13), or a combination of these three.
- a design of the obstacle device as well as its obstacles and / or passage openings and the mixing trough in dependence on a (bio) -chemical process to be performed with the mixer take place.
- Fig. 2b above shows the mixer 20 of Fig. 2a above. While in Fig. 2a above the mixer 20 is in a second phase, for example a phase of low angular velocity, the mixer 20 in Fig. 2b is at the top in a first phase, for example a phase of high angular velocity of the rotor. It can be seen from the length of the arrow 18 that an amount of the centrifugal force F z in FIG. 2b above (ie in the first phase) is greater than the amount of the centrifugal force F z in FIG. 2a above (in the second phase). In particular, it will be seen that the magnitude of the centrifugal force F z in FIG. 2b above is greater than the amount of the restoring force F r .
- a spring constant of the spring 16 is independent of the angular velocity of the rotor.
- the mixing trough 11 and thus the mixing trough section 14 are located radially further outward in FIG. 2b than was the case in FIG. 2a above.
- the distance Li between the wall portion 14 of the mixing trough 11 and the Hindemis recognized 12 in Fig. 2b above at the second phase is greater than in Fig. 2a above at the first phase.
- the larger centrifugal force F z in the second phase can be achieved by a higher angular velocity of the rotor relative to the first phase.
- the mixing trough 11 moves to a location located radially further outward, and with it the liquid 15, which passes through the passage openings 13 of dernis prepared 12 flows through and the obstacles of the obstacle device 12 flows around.
- the spring 16 is compressed.
- the obstacle device 12 is fully extended from the mixing trough 11 and is no longer in contact with the liquid 15, so according to further exemplary embodiments, the mixing trough 11 may be formed so that even at a maximum deflection of the Mixing trough 11 opposite the obstacle device 12, the obstacle device 12 is not extended from the mixing trough 11.
- Fig. 2b below shows analogously to Fig. 2b above the mixer 21 in a first phase in which a sum of the centrifugal force F z generated by the rotation of the rotor is greater than the amount of return force F R generated by the spring 16.
- the distance Li between the wall section 14 'and the obstacle device 12' is also larger in Fig. 2b below the distance L ⁇ between the wall portion 14 'and the obstacle device 12' in Fig. 2a below.
- the spring 16 is also compressed here.
- the mixer 21 therefore does not differ in its function from the mixer 20.
- the mixer 21 can be used in particular for mixing liquids of different densities.
- Fig. 3 above shows a mixer 30 for insertion into a rotor of a centrifuge according to an embodiment of the present invention.
- the mixer 30 differs from the mixer 20 shown in FIGS. 2 a and 2 b in that the wall section of the mixing trough 11, the distance of which is variable with respect to the obstacle device 12, is formed as an elastic membrane 22.
- the elastic membrane 22 thus also forms the return means.
- the mixer 30 therefore has no spring 16 to generate the restoring force against the centrifugal force.
- the obstacle device 12 can be arranged on a non-elastic part of the mixing trough 11 or on the housing 17 (as shown in Fig. 3 above).
- the elastic diaphragm 22 may radially expand outwardly based on the centrifugal force generated by the rotation of the rotor about the rotation axis 140, so that the distance of the elastic diaphragm 22 from the obstacle device 12 varies.
- Fig. 3 above shows with a dotted line the elastic membrane 22 in a first state at a low angular velocity. Furthermore, FIG. 3 shows at the top with a dashed line the elastic membrane 22 in a second state, at a higher angular velocity of the rotor than the first state. Further, Fig. 3 shows at the top with a solid line the elastic membrane 22 in a third state at an even higher angular velocity of Rotor, as in the second state.
- a dotted arrow 18a is thereby an amount of the centrifugal force F z in the angular velocity in the first state
- a dashed arrow 18b this case indicates an amount of centrifugal force F z in the angular speed of the rotor in the second state
- a solid arrow 18c are thereby an amount of the centrifugal force F z at the angular velocity in the third state.
- the amount of centrifugal force F z is greater than the amount of restoring force F r in the first state, whereby the elastic membrane 22 stretches away from the obstacle device 12 and the liquid 15 passes through the passage openings 13 of the obstacle device 12 flows therethrough.
- the liquid 15 flows around the obstacles (between the passage openings 13) of the obstacle device 12, which results in thorough mixing.
- the angular velocity of the rotor is further increased and thus the amount of centrifugal force F z is greater than in the second state, whereby the elastic membrane 22 continues to expand, and thus the distance L [between the elastic membrane 22 and the obstacle device 12 further increased.
- the liquid 15 urges the elastic membrane 22 radially outward, flowing in a first direction through which the passage openings 13 of the obstruction device 12 flow doing the obstacles of the obstacle device 12th
- the elastic membrane 22 pushes the liquid 15 in a second direction through the passage openings 13 of the obstacle device 12 through the obstacles of the obstacle device 12 (in the second direction).
- FIG. 3 shows a mixer 31 according to a further exemplary embodiment of the present invention.
- the mixer 31 differs from the mixer 30 shown in FIG. 3 in that it has an inclined obstacle 12 '. Furthermore, through openings 13 of the obstacle device 12 'have different opening cross sections or, in other words, distances between obstacles of the obstacle device 12' vary along a propagation direction of the obstacle device 12 '.
- the oblique obstacle device 12 ' has already been explained below with reference to FIGS. 2a and 2b below, a repeated description is therefore omitted.
- An elasticity of the elastic membrane 22 of the mixing trough 11 of the mixer 30 and the mixer 31 is higher than an elasticity of the wall portion 14 of the mixing trough 11 of the mixer 20 and the mixer 21.
- the wall portion 14 of the mixing trough 11 may for example be formed of a hard plastic material
- the elastic membrane 22 may for example be formed of a soft plastic material, for example an elastomeric material.
- the spring 16 of the mixers 20 and 21 may, for example, be formed of the same elastic material as the elastic membrane 22 of the mixers 30, 31.
- a coefficient of elasticity or a spring force coefficient of the spring 16 and the elastic membrane 22 may be the same, for example, such that a restoring force generated by the spring 16 is identical to a restoring force generated by the elastic membrane 22.
- the elastic membrane 22 may be configured to emerge in response to a given angular velocity of the rotor, thereby releasing the liquid 15 in the mixing trough 11.
- An amount of angular velocity required to travel the elastic membrane 22 may be greater than amounts of angular velocities used in mixing the liquid 15.
- the amount of angular velocity needed to travel the elastic membrane 22 may be greater than the amount of angular velocity of the rotor in the third state, indicated by the solid lines.
- FIG. 4 shows the mixer 30 from FIG.
- the mixer 30 shown in FIG. 4 furthermore having a mandrel 32, which is arranged radially further outward than the elastic membrane 22 during the rotation of the rotor.
- the mandrel is designed to puncture the elastic membrane 22 at a given angular velocity, so that the liquid 15 located in the mixing trough 11 in FIG. 5 is released.
- the elastic membrane 22 may, for example, stretch so far that the mandrel 32 is retracted into it and thus pierces the elastic membrane 22.
- An amount of angular velocity required for retracting the mandrel 32 may be greater than an amount of a maximum mixing angular velocity.
- the amount of angular velocity needed for retraction of the mandrel 32 may be greater than the amount of angular velocity in the third state of the mixer 30 shown in solid lines in FIGS. 3 and 4.
- the liquid 15 released by the migration of the membrane 22 or the piercing of the membrane 22 can be located, for example, within the housing 17 of the mixer 30 or via passage openings 33 or a passage opening 33 of the mixer 30, for example on a floor of the housing 17 leave the mixer 30, for example, to flow in a cavity of a downstream body.
- a mixer according to an embodiment of the present invention may comprise sedimentation cavities, for example in a mixing trough.
- sedimentation cavities for example in a mixing trough.
- Fig. 5 shows a mixer 40 according to another embodiment of the present invention.
- the mixer 40 shown in FIG. 5 differs from that shown in Fig. 2a above mixer 20 in that not the mixing pan is movably 11, special * 30 which Hindemis noticed 12 (here designed as a perforated plate 12) movable in the Mischwanne 11 is stored.
- the mixing trough 11 is locked to a housing 17 of the mixer 40.
- the Hindemis Rhein 12 is therefore movably mounted to the mixing trough 11 and movable to the housing 17 of the mixer 40.
- the spring 16 is disposed between the wall portion 14 whose distance Li to the hindrance device 12 is variable, and 35 of the hindrance device 12.
- the Hindemis worn 12 within the mixing trough 11 up and down (from radially inward to radially outward and backward) and thereby travels through the liquid 15.
- the liquid 15 is not moved from a radially inner to a radially outer location, but the obstacle device 12 (the orifice plate 12).
- the obstacle device 12 By moving the obstacle device 12, the liquid 15 flows through through openings 13 of the obstacle device 12 therethrough.
- the liquid 15 flows around obstacles 9 (shown hatched in Fig. 5) of the obstacle device 12, whereby a mixing effect is achieved.
- Fig. 6 shows a sectional view of a device 700 for insertion into a rotor of a centrifuge.
- the device 700 has a mixer 730 according to an exemplary embodiment of the present invention in a cavity 160 a of a second body 120.
- the mixer 730 may also be referred to below as a mixing device 730.
- the device 700 has three bodies 110, 120, 510, which are arranged in a stacking direction in a housing 130, wherein upon rotation of the device 700 about a rotation axis 140, a first body 110 at the radially farthest inward and a fourth body 510 at radially farthest outward.
- the second body 120 is disposed between the first body 110 and the third body 510.
- the device 700 is configured such that, in response to rotation of the rotor, the second body 120 may rotate relative to the first body 110 and the second body 510.
- a coupling of different cavities of the first body 110 with the cavity 160a of the second body 120 based on a rotation of the rotor can be achieved in different phases.
- the first body 110 has eight cavities, for example as reagent pre-storage chambers.
- the second body 120 has in its cavity 160a the mixing device 730 (the mixer 730), which is designed to mix at least two fluids located in the cavity 160a in response to a rotation of the rotor.
- the third body 510 has a first cavity 720 and a second cavity 720b.
- the first cavity 720a of the third body 510 may be, for example, an eluate collection vessel or an eluate chamber
- the second cavity 720b of the third body 510 may be, for example, a so-called waste container or a waste chamber.
- the housing 130 has two housing parts 132, 134 which can be separated from one another, so that when these two housing parts 132, 134 are separated, at least one of the bodies of the device 700 (for example the third body 510) can be removed from the device 700.
- the housing 130 may also include a plurality of housing parts 132, 134. The individual housing parts 132, 134 may, for example, be inserted into one another via springs and grooves or else via Screwed screwed together.
- a first housing part 132 of the two housing parts 132, 134 of the housing 130 may also be referred to as a first sleeve 132, and a second housing part 134 of the two housing parts of the housing 130 may also be referred to as a second sleeve 134.
- a first sleeve 132 A first housing part 132 of the two housing parts 132, 134 of the housing 130 may also be referred to as a first sleeve 132
- a second housing part 134 of the two housing parts of the housing 130 may also be referred to as a second sleeve 134.
- the second sleeve 134 is slipped onto the first sleeve 132.
- first body 110 may be referred to as a first turret 110
- second body 120 as a second turret 120
- third body 510 as a third turret 510.
- the first turret 110 has, as already described above, a reagent pre-storage.
- the second turret 120 has the mixing device 730 as already described above.
- the third turret 510 has, as already described above, an eluate chamber 720a and a waste chamber 720b.
- the device 700 has a spring 710 for the lateral movement of the three revolvers 110, 120, 510.
- the spring 710 serves to generate a restoring force which opposes a centrifugal force generated by the rotation of the rotor to allow a shifting operation (for example, a rotation of the second revolver 120 with respect to the other two revolvers).
- the spring 710 may be similar to a return spring for a ballpoint pen, a twist of the second turret 120 with respect to the other two turrets 110 and 510 may be based on a ballpoint pen mechanism.
- the device 700 shown in FIG. 6 with three revolvers 110, 120, 510 can be used, for example, for the purpose of DNA extraction.
- a ballpoint pen mechanism may translate the centrifugation protocol into a stepwise rotation of the second turret 120 relative to the first turret 110 and the third turret 510.
- the spring 710 below the third turret 510 regulates the distance to the casing or to the housing 130, which has (or consists of) the two housing parts 132, 134.
- the spring 710 may be formed as a compression spring or tension spring.
- the spring 710 may also be formed as another return means, which generates a restoring force on at least one body of the device 700 '5.
- elastomers rubber band
- metal springs metal springs
- thermoplastics or thermosets can be used as restoring means, for example.
- the return means may be manufactured as part of a body (for example as part of the third body 510). Such manufacturing methods are known from the packaging industry and z. For example, in the manufacture of disgusting tablets of tablets by injection molding. In this way, both the number of parts can be reduced, and the assembly can be simplified.
- FIG. 7a shows on the left the first housing part 132 of the housing 130 in a side view and a sectional view along a section axis A-A. Furthermore, FIG. 7a on the right shows the second housing part 134 of the housing 130 in a side view and a sectional view along a section axis A-A.
- the second housing part 134 forms a lower end of the device 700, d. H. during a rotation of the device 700, the second housing part 134 is arranged radially outermost, and in particular radially further outward than the first housing part 132.
- the first housing part 132 has a cylindrical shape and a circular cross section.
- the first housing part 132 On a base side 804 of the first housing part 132, the first housing part 132 has two opposing hooks 810.
- the two opposed hooks 810 are configured to be received in two opposing hook receivers 812 of the second housing 134.
- the two hooks 810 project beyond the base side 804 of the first housing part
- the housing portion 132 may include a viewing window 814 (eg, on a transparent plastic material) which, for example, in combination with a display on the second body 120, provides a phase indication to indicate a phase in which the device 700 is located at the time of tapping ,
- the first housing part 132 may have on an inner side a plurality of guide grooves 816, which extend in at least a partial region of the inner region of the first housing part 132 in a direction orthogonal to a cover side 802 of the first housing part 132.
- the inner grooves of the first housing part 132 may be accessible, for example, from the base side 804 of the first housing part 132, for example around the three turrets 110, 120, 510 into the first one Housing part 132.
- the first housing part 132 on its cover side 802 may be open or closed and may, for example, have a lid on the top 802.
- the second housing part 134 has on a cover side 806 the same circular cross-section as the first housing part 132 on its base side 804.
- the hook receivers 812 adapted to the hooks 810 of the first housing part 132, are set back from the cover side 806 of the second housing part 134 on the second housing part 134.
- the circular cross-section of the second housing part 134 can taper in a region in which the hook receptacles 812 no longer extend to a base side 808 of the second housing part 134, d.
- the housing part 134 may be frusto-conical on an end opposite the cover side 806. Within the frusto-conical end, the housing part 134 may have a receptacle 818 for the spring 710.
- An inner region of the second housing part 134 may be accessible from the cover side 806 of the second housing part 134, for example to receive the third body 510, or to remove it from the housing 130.
- a length from the cover side 802 to the base side 804 of the first housing part 132 may be greater than a length from the cover side 806 to the base side 808 of the second housing part 134.
- FIG. 7b shows schematic representations of the first body 110 of the device 700 according to FIG. 6.
- FIG. 7b-a shows the first body 110 and the first revolver 110 in a side view.
- the first body 110 is a cylindrical body 110 having a top side 820 and an opposite bottom side 822.
- the first body 110 has a plurality of guide springs 824 on its outside.
- the number of guide springs 824 may, for example, be adapted to the number of guide grooves 816 of the first housing part 132 (ie of the housing 130).
- the guide springs 824 of the first body 110 are configured to engage with the guide grooves 816 of the housing part 132.
- the guide springs 824 may be configured (in conjunction with the guide grooves 816 of the first housing portion 132) to permit rotation of the first body 110 with respect to the other bodies 120, 510 (eg, transitioning from a first phase to a second phase) prevent.
- the guide springs 824 of the first body 110 may be chamfered on the cover side 820 facing ends, for example, a simpler insertion of the first body 110 in to allow the housing 130 (ie in the second housing part 134). Due to the tapered ends of the guide springs 824, wedging of the guide springs 824 with the guide grooves 816 of the first housing 132 upon insertion of the first body 110 is precluded (or at least almost eliminated).
- the first body 110 may have on its base side 822 a plurality of profile teeth 826, which are arranged circumferentially around the first body 110.
- a number of the profile teeth 826 may be adapted to a number of process steps to be performed in the device.
- a number of the teeth may vary in different devices which are suitable for different (bio) chemical processes.
- the number of guide springs 824 and the guide grooves 816 may vary.
- the first housing part 132 has eight guide grooves 816.
- the first body 110 has eight guide springs 824 and eight profile teeth 826.
- the profile teeth 826 may be formed, for example, to allow a guide of the second body 120 and the second turret 120.
- Fig. 7b-a shows in a side view of the first turret 110 structures for the ballpoint pen mechanism with grooves between guide springs 824 for guiding in the column (in the first housing part 132) and recesses (profile teeth 826) for guiding the second turret 120th
- FIG. 7b-b shows a top view of the first turret 110 with a multiplicity of cavities for the preliminary reagent storage.
- the first turret 110 has eight cavities. In the eight cavities, for example, eight different reagents can be pre-stored for processing.
- FIG. 7 b-c shows a bottom view of the first revolver 110 with tracks of three spikes, which are arranged, for example, on the second revolver 120 for opening closure means of the cavities of the first revolver 110.
- the three spikes pierce each of the chambers (the cavities) with the upstream reagents.
- FIG. 7b-c the respective paths that make up the individual mandrels in the rotation of the second body 120 with respect to the first body 110 are shown.
- a path of a first mandrel 828a is shown with a dotted arrow.
- a path of a second mandrel 828b is shown with a dashed arrow and a path of a third mandrel 828c is shown with a solid arrow.
- the individual numbers in the respective cavities indicate both in FIGS. 7b-b and in FIG. 7b-c, in which phase, that is also in which order, the individual cavities or their closure means of one of Spikes are being cut.
- a first cavity 150a of the first body 110 is pierced by the first mandrel 828a in a first phase.
- a liquid or a process agent located in the first cavity 150a of the first body 110 can then flow into a cavity of the second body 120.
- a second cavity 150 b of the first body 110 is pierced by the first mandrel 828 a, so that one in the The second cavity 150 b of the first body 110 liquid can flow into a cavity of the second body 120 (for example, in the same cavity in which even the liquid from the first catality 150 a of the first body 110 has flowed).
- a third cavity 150c is pierced by the first mandrel 828a, so that a liquid located in the third cavity 150c can flow into a cavity of the second body 120.
- the first mandrel 828a may in this case be connected to a cavity of the second body 120, so that liquids of cavities which have been pierced by the first mandrel 828a all flow into one and the same cavity within the second body 120.
- a seventh cavity 150 g of the first body 110 is pierced by the second mandrel 828 b, so that a liquid located in the seventh cavity 150 g flows into a cavity of the second body 120.
- an eighth cavity 150h of the first body 110 is pierced by the second mandrel 828b such that a liquid located in the eighth cavity 828a enters a cavity of the second body 120 (e.g., the same cavity into which the liquid from the seventh cavity has flowed 150g) flows.
- the second mandrel 828b may be configured analogously to the first mandrel 828a such that liquids from cavities pierced by the second mandrel 828b flow into a common cavity in the second body 120 or at least via a common fluid path in the second body 120 run.
- a fourth cavity 150d is pierced by the third mandrel 828c so that a liquid located in the fourth cavity 150d flows into a cavity of the second body 120.
- further reagents may be upstream, or no reagents may be upstream.
- the mandrels can be arranged offset on the second body 120, and the closure means of the respective cavities only at certain locations, which are shown in FIGS. 7b-b and 7b-c are marked hatched, pierceable by the thorns. Furthermore, it is also possible that the individual mandrels 828a, 828b, 828c in a phase in which they are needed from the second body 120th be extended and retracted in another phase in the body 120. This can be initiated, for example, via the centrifugation protocol.
- FIG. 7c shows the second body 120 (the second revolver 120) from different views.
- Fig. 7c-a shows the second body 120 in a side view.
- Fig. 7c-b shows the second body in a sectional view along a section axis A-A.
- Fig. 7c-c shows the second body 120 in an isometric view.
- Fig. 7c-d shows the second body 120 in a plan view.
- Fig. 7c-e shows the second body 120 in a further sectional view along a section axis B-B.
- the second body 120 forms a housing of the mixing device 730 or the mixer 730.
- a mixing trough 835 of the mixer 730 and an obstacle device 840 (here designed as a hole trough 840) of the mixer 730 are located in the cavity 160a of the cylindrical housing (the second body 120). are arranged.
- the second body 120 is a cylindrical body having a top side 830 and a base side 832 opposite thereto.
- the second body 120 has on its top side 830, which may also be referred to as a lid, the three pins 828a, 828b, 828c.
- the three mandrels have a different distance from a rotation axis 250 of the body 120.
- the first mandrel 828a is farthest from the axis of rotation 250, and the third mandrel 828c is least distant from the axis of rotation.
- the second body 120 further includes a plurality of guide springs 834 disposed on an outer side of the second body 120. In the embodiment shown in FIG. 7 c, the second body 120 has four guide springs 834.
- the guide springs 834 project beyond the top side 830 of the second body 120 and each have beveled ends in an end region in which they project beyond the top side 830.
- the guide springs are configured to interengage with the tread teeth 826 of the first body 110 and the guide grooves 816 of the housing 130 upon transition from one phase of the device 700 to a next phase (eg, from the first phase to the second phase) ,
- a number of the guide springs 834 may depend on the number of process steps to be performed for a process for which the device 700 is provided.
- the second body 120 comprises the mixing device 730 or, in other words, the second body 120 forms a housing of the mixing device 730.
- the mixing device 730 is designed to form at least two different fluids or liquids within the cavity 160a of the second body 120 to mix.
- the cavity 160a of the second body 120 can therefore also be referred to below as the mixing chamber 160a be designated.
- the mixing device 730 has within the mixing chamber 160a a first mixing spring 836 (comparable to the spring 16 of the mixer 20 according to FIG. 2a above) for mixing.
- the mixing device 730 has the hole trough 840 locked in the mixing chamber 160a on the first body 120 (comparable to the obstacle 12 of the mixer 20 according to FIG.
- the well 840 or obstruction 840 may also be referred to as the orifice plate 840.
- the openings 845 of the hole trough 840 are arranged on the hole trough 840 such that when the device 700 is received in a rotor, a centrifuge, and with a rotation of the rotor, the openings 845 are arranged radially outermost with respect to the hole trough 840.
- the well 840 may be open to the top 830 of the second body 120 so that liquid may flow from a cavity of the first body 110 into the cavity 160a of the second body 120, and thus into the well 840.
- the mixing device 730 has a mixing trough 835 (comparable to the mixing trough 11 of the mixer 20 according to FIG. 2 a) or a mixing bowl 835 in the mixing chamber 160 a.
- the mixing trough 835 is movably mounted with respect to the hole trough 840 within the mixing chamber 160a.
- the mixing trough 835 is arranged such that upon a rotation of the device 700, the mixing trough 835 (or at least one wall section 14 of the mixing trough 835) is arranged radially further outside than the hole trough 840.
- a liquid which may be in the well 840 may flow from the well 840 into the mixing well 835 through the openings 845 due to the centrifugal force created by the rotation.
- the hole trough 840 and the mixing trough 835 are designed in such a way that, when the mixing trough 835 moves, the hole trough 840 can be retracted into the mixing trough 835.
- the mixing trough 835 therefore has a larger cross-section than the hole trough 840 to accommodate the hole trough 840 as the mixing trough 835 moves.
- the mixing trough 835 has an elevation 846 for receiving the first mixing spring 836.
- the hole trough 840 has an elevation 848, which is adapted to the elevation 846 of the mixing trough 835, so that the hole trough 840 can be received by the mixing trough 835 when the mixing trough 835 moves toward the perforated plate 840.
- the first mixing spring 836 is arranged between the mixing trough 835 and the second body 120 (the housing of the mixing device 730) in order to exert an opposing force acting on the centrifugal force restoring force on the mixing trough 835.
- the mixing trough 835 may have a hole 841 or a plurality of holes 841 with a closing means, such as a cover foil 847.
- a hole 841 may also be referred to as a through opening 841 of the mixing trough 835.
- the hole 841 of the mixing trough 835 is arranged on the mixing trough 835 such that upon rotation of the rotor, the hole 841 is arranged radially outermost with respect to the mixing trough 835.
- a mandrel 833 may be disposed on the second body 120. In this case, the mandrel 833 can be arranged on the second body 120 in order to puncture the cover foil 847 of the hole 841 in response to a given angular speed of the rotor. Mandrel 833, in conjunction with hole 841 and cover sheet 847, thus forms a valve of mixing tub 835 and also mixing chamber 160a of second body 120.
- Mixing device 730 may further include a second mixing spring 837 within mixing chamber 160a.
- the second mixing spring 837 may be disposed between the mixing trough 835 and the second body 120, wherein a spring constant of the second mixing spring 837 may be greater than a spring constant of the first mixing spring 836. That is, a restoring force generated by the first mixing spring 836 is less than a restoring force generated by the second mixing spring 837.
- the mixing trough 835 in the wall section 14 may have at least one passage opening 841 with a cover foil 847.
- the mixing device 730 may include a mandrel 833 configured to pierce the lid sheet 847 in response to a given angular velocity.
- An angular velocity of the rotor required for the piercing of the lidding film 847 is greater than an amount of angular velocity required for the mixing of liquids present in the mixing trough 835.
- a maximum mixing angular velocity of the rotor may be referred to as a first angular velocity of the rotor, and a minimum mixing angular velocity, for example, at which the distance L] between the well 845 and the wall portion 14 of the mixing trough 835 is minimum, will be referred to as the second angular velocity of the rotor.
- a third angular speed of the rotor which is required for the piercing of the cover film 847 with the mandrel 833, is greater than the first angular velocity and the second angular velocity of the rotor.
- the distance Li between the wall portion 14 and the hole trough 845 is even greater than at the first angular velocity of the rotor. While the first angular velocity and the second angular velocity of the rotor can be achieved several times during a mixing process, for example to produce a multiple movement of the mixing trough 835 in the cavity 160a, the third angular velocity of the rotor is typically only reached once, since after opening the cover film 847, the liquid in the mixing tub 835 leaves the mixing trough 835 and no further mixing within the mixing trough 835 is possible. Furthermore, the second body 120 may have a drip-off nose 843 on its base 832.
- the first mixing spring 836 moves the mixing trough 835 up and down within the cavity 160a (the mixing chamber 160a), whereby a liquid in the mixing chamber 160a is in communication with another in the mixing chamber 160a Liquid is mixed.
- the mixing trough 835 is moved by the changing centrifugal force with a change in the angular velocity of the rotor and the restoring force of the first mixing spring 836 acting in the opposite direction to the centrifugal force.
- the mixing tub 835 is moved radially outward by the centrifugal force to a point, and the first mixing spring 836 counteracts this movement.
- the mixing trough 835 moves back and forth.
- a liquid present in the mixing trough 835 is transported through the openings 845 of the hole trough 840 with each movement of the mixing trough 835.
- the liquid flows through the openings 845 of the hole trough 840 with a variable spring length, whereby a mixing process takes place.
- This mixture is realized by the interaction of centrifugal force and restoring force (generated by the first mixing spring 836).
- the change in the rotational frequency of the centrifuge moves the mixing trough (or mixing bowl) 835 from a radially further inward to a radially outward location, and vice versa.
- the liquid present in the mixing trough 835 is guided through the openings 845 of the hole trough 840 and flows around the edges of the openings 845, that is to say the obstacles 9 of the perforated trough 840, which results in thorough mixing.
- the second mixing spring is used to switch the valve (formed from the hole 841, the cover sheet 847 and the mandrel 833).
- the second mixing spring 837 has a higher spring constant than the first mixing spring 836.
- a holding force generated by the second mixing spring 837 is thus larger than the restoring force generated by the first mixing spring 836.
- the second mixing spring 837 is compressed only at comparatively high rotational frequencies of the centrifuge, so that the mixing trough 835 moves radially outward to the mandrel 833, so that the mandrel 833 opens the lid foil 847 of the hole 841.
- An angular velocity required for the compression of the second mixing spring 837 (for example the third angular velocity described above) of the rotor of the centrifuge can be greater than the angular velocity (for example the first angular velocity) of the rotor required for a compression of the first mixing spring 836.
- an amount of a holding force generated by the second mixing spring 837 at the first angular velocity and the second angular velocity is larger than amounts of the component of the centrifugal force opposing the restoring force.
- the amount of the holding force is smaller than an amount opposite to the restoring force-acting component of the centrifugal force.
- a spring constant of the first mixing spring 836 may be greater than a spring constant of the spring 710 which serves to rotate the second body 120 with respect to the other two bodies 110, 510 of the device 700.
- the liquid in the mixing trough 835 can exit the second turret 120 via a column 838 (for example via a silicate column 838) in the mixing chamber 160a through the drip nose 843 and, for example, into the waste collection container (into the waste bin) Waste chamber) 720b or into the eluate collection container (into the eluate chamber) 720a of the third body 510.
- a column 838 for example via a silicate column 838
- the mandrels 828a, 828b, 828c may have on the top side 830 of the second body 120 fluid guides, for example in the form of funnels and subsequent channels, or in the form of bevels so as to provide different paths for fluids whose cavities they pierce within the mixing chamber 160a , enable.
- fluids released by the first mandrel 828a may be directed into the well 840 with a first fluid guide 829a formed as a bevel.
- Fluids released from the second mandrel 828b may be provided, for example, with a second fluid guide 829b serving as a Funnel with a channel that leads past the hole trough 840 and the mixing trough 835, is formed on the column 838 or in a region of the mixing chamber 160 a, outside the mixing trough 835, passed.
- the region may be fluidly connected to the column 838 so that the fluid flows from the region to the column 838.
- Fluids released from the third mandrel 828c may, for example, be passed directly over the column 838 with a third fluid guide 829c, which is also formed as a funnel with a channel passing the hole trough 840 and the mixing trough 835 ,
- the channel of the third fluid guide 829c may have a smaller cross-section than the channel of the second fluid guide 829b, for example such that a fluid flows slower through the third fluid guide 829c than through the second fluid guide 829b.
- the mixing chamber 160a may be frusto-conical in a region below the mixing trough 835 (radially further outward than the mixing trough 835), for example, around a funnel toward the drip hare 843 for the fluids in the mixing chamber 160a.
- the valve in the mixing chamber 160a may also be designed as a predetermined breaking point or a siphon, for example to mix a plurality of liquids or reagents from the first body 110 within the mixing chamber 160a, and in a predetermined process step this valve or to open the predetermined breaking point or siphon so that the mixed reagents can exit the mixing chamber 160a (eg, via the dripping hare 843).
- the lidding film 847 may be formed in the wall portion 14 of the mixing trough 835 so as to travel in response to the third angular velocity whose magnitude is greater than the magnitude of the first angular velocity and the magnitude of the second angular velocity. The mandrel 833 would then no longer be needed, which would result in a simpler manufacture of the mixing device 730.
- the mixing chamber 160a may have a (chromatographic) column 838 at an exit (at the drip hare 843) facing the base 832, such as required for DNA extraction to form reagents.
- a mixed liquid can, as described above, be passed through the column 838 via a valve or via a predetermined breaking point or via a siphon.
- the mixing chamber 160a may include a foil 847 or a membrane 847 which extends from one within the second body 120 located mandrel 833 can be pierced in response to a given angular velocity of the rotor.
- the mixing trough 835 can be locked in the second body 120 or mounted on the second mixing spring 837.
- the hole trough 840 based on the variable angular velocity of the rotor, within the mixing trough 835 move up and down.
- the first mixing spring 836 may be arranged between the mixing trough 835 and the hole trough 840.
- the second body 120 may comprise a plurality of cavities and thus also a plurality of mixing chambers, for example with separate mixing devices.
- the second body 120 may have on its outer side a scale display 842 which, for example in conjunction with the viewing window 814 of the first housing part 132, may form a phase display of the device 700.
- the scale display 842 may simply consist of letters and / or numbers indicating a phase of the device 700.
- Fig. 7d shows the third body 510 (the third turret 510) in two different views.
- Fig. 7d-a shows the third body 510 in a side view and
- Fig. 7d-b shows the third body 510 in an isometric view.
- the third body 510 is a cylindrical body having a top side 850 and a base side 852 opposite thereto.
- the third body 510 has, as already described with reference to FIG.
- the third body 510 has guide springs 854 on its outer side, for example to prevent a rotation of the third body 510 during a transition from one phase to a next phase of the device 700.
- the third body 510 may be formed to be detachable from the housing 130, for example, to perform further processing of the liquid collected in the eluate chamber 720a.
- a mixer may also have sedimentation cavities in which bacteria and other solids can be sedimented. These bacteria and solids can have a greater density than a liquid mixture which is suitable for further use from the mixer or the mixed tub is removable.
- embodiments of the present invention also allow for sedimentation of insoluble cell constituents of the liquids or higher density constituents than the liquids themselves.
- Embodiments of the present invention can be made particularly easily, for example, in an injection molding process from a plastic material.
- Exemplary embodiments of the present invention can be produced, for example, as disposable articles.
- embodiments of the present invention allow for improved mixing of liquids over standard reaction vessels, such as simple centrifuge tubes.
- a restoring force generated by a restoring means is perpendicular to an axis of rotation of the rotor of the centrifuge. This is typically the case when using a mixer in a holder of a rotor of a swing-out centrifuge.
- a restoring force F r generated by a return means is not perpendicular to the axis of rotation 140.
- a centrifugal force F z generated by the rotation 140 does not act directly opposite to the restoring force F r .
- embodiments of the present invention may be adapted to be received in both rotatable swing-wing rotors and swing-arm rotors of solid angle centrifuges.
- a restoring force F r generated in a mixer can act counter to a centrifugal force generated by the rotation of the rotor or against a component of the centrifugal force generated by the rotation of the rotor.
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mixers Of The Rotary Stirring Type (AREA)
- Centrifugal Separators (AREA)
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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DE102010003224.7A DE102010003224B4 (de) | 2010-03-24 | 2010-03-24 | Mischer zum Einsetzen in einen Rotor einer Zentrifuge |
PCT/EP2011/054115 WO2011117148A1 (de) | 2010-03-24 | 2011-03-18 | Mischer zum einsetzen in einen rotor einer zentrifuge |
Publications (2)
Publication Number | Publication Date |
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EP2536490A1 true EP2536490A1 (de) | 2012-12-26 |
EP2536490B1 EP2536490B1 (de) | 2014-09-10 |
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Application Number | Title | Priority Date | Filing Date |
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EP11710456.2A Not-in-force EP2536490B1 (de) | 2010-03-24 | 2011-03-18 | Mischer zum einsetzen in einen rotor einer zentrifuge |
Country Status (4)
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US (2) | US20130015114A1 (de) |
EP (1) | EP2536490B1 (de) |
DE (1) | DE102010003224B4 (de) |
WO (1) | WO2011117148A1 (de) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8905624B1 (en) * | 2009-08-20 | 2014-12-09 | Harold W. Howe | Control of vibratory/oscillatory mixers |
DE102011007779A1 (de) | 2011-04-20 | 2012-10-25 | Robert Bosch Gmbh | Mischkammer, Kartusche sowie Verfahren zum Mischen einer ersten und zweiten Komponente |
DE102012213756A1 (de) * | 2012-08-03 | 2014-02-06 | Robert Bosch Gmbh | Reagenzgefäß-Einsetzteil, Einsetzkomponente und Reagenzgefäß |
DE102012213757A1 (de) | 2012-08-03 | 2014-02-27 | Robert Bosch Gmbh | Reagenzgefäß-Einsetzteil, Reagenzgefäße, Verfahren zum Zentrifugieren mindestens eines Materials und Verfahren zum Druckbehandeln mindestens eines Materials |
DE102013200352A1 (de) * | 2013-01-14 | 2014-07-17 | Robert Bosch Gmbh | Kartusche, Zentrifuge für diese Kartusche sowie Verfahren |
DE102013220064B3 (de) * | 2013-10-02 | 2014-12-24 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Vorrichtung und verfahren zum bewegen einer festphase in eine mehrzahl von kammern |
DE102013220257B3 (de) | 2013-10-08 | 2015-02-19 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Vorrichtung und verfahren zur durchmischung zumindest einer flüssigkeit |
CN109406403A (zh) * | 2018-09-29 | 2019-03-01 | 天津诺塑料制品有限公司 | 一种便于消毒杀菌的生物检测试剂桶 |
CN112387167A (zh) * | 2021-01-20 | 2021-02-23 | 广州中安基因科技有限公司 | 一种化妆品生产用高效搅拌设备 |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US209205A (en) * | 1878-10-22 | Improvement in egg-beaters | ||
US212263A (en) * | 1879-02-11 | Improvement in churns | ||
US200271A (en) * | 1878-02-12 | Improvement in devices for use in reciprocating churns | ||
DE524254C (de) * | 1929-12-25 | 1931-05-05 | Max Mueller Maschinen Und Form | Mischvorrichtung fuer Gummi-Rohmassen |
US2198256A (en) * | 1938-04-11 | 1940-04-23 | Henri G Levy | Centrifuge tube |
US2507309A (en) * | 1945-11-26 | 1950-05-09 | Larsson Gustav Allan | Centrifuge |
US3401876A (en) * | 1966-07-25 | 1968-09-17 | Dade Reagents Inc | Mixing and decanting centrifuge |
US4001122A (en) * | 1973-08-22 | 1977-01-04 | Telan Corporation | Method and device for separating blood components |
US3905528A (en) | 1974-04-10 | 1975-09-16 | Hugh V Maiocco | Two-piece concentric centrifuge sample container |
US3929646A (en) * | 1974-07-22 | 1975-12-30 | Technicon Instr | Serum separator and fibrin filter |
IL74967A (en) | 1985-04-18 | 1988-10-31 | Assaf Pharmaceutical Ind | Separation of materials from a liquid dispersion by sedimentation |
US4857187A (en) * | 1987-09-28 | 1989-08-15 | The Government Of The U.S. As Represented By The Secretary Of The Department Of Health And Human Services | Multistage mixer-settler centrifuge |
US4900435A (en) * | 1988-06-09 | 1990-02-13 | Large Scale Biolocy | Centrifugal fast chromatograph |
DE19838540C2 (de) * | 1998-08-25 | 2001-07-26 | Herbert Huettlin | Verfahren zum Behandeln eines partikelförmigen Guts mit einem Überzugsmedium sowie Vorrichtung zur Durchführung eines derartigen Verfahrens |
US6595680B2 (en) * | 2000-02-24 | 2003-07-22 | Kubota Corporation | Food mixing apparatus |
WO2001081002A1 (en) * | 2000-04-18 | 2001-11-01 | Large Scale Proteomics Corporation | Method and apparatus for making density gradients |
ES2298234T3 (es) * | 2000-04-28 | 2008-05-16 | Harvest Technologies Corporation | Disco separador de componentes sanguineos. |
WO2002009840A1 (en) * | 2000-07-28 | 2002-02-07 | Large Scale Proteomics Corporation | Method and apparatus for unloading gradients |
DE102010003223B4 (de) * | 2010-03-24 | 2014-09-18 | Albert-Ludwigs-Universität Freiburg | Vorrichtung zum Einsetzen in einen Rotor einer Zentrifuge, Zentrifuge und Verfahren zum fluidischen Koppeln von Kavitäten |
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2010
- 2010-03-24 DE DE102010003224.7A patent/DE102010003224B4/de not_active Expired - Fee Related
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2011
- 2011-03-18 WO PCT/EP2011/054115 patent/WO2011117148A1/de active Application Filing
- 2011-03-18 EP EP11710456.2A patent/EP2536490B1/de not_active Not-in-force
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2012
- 2012-09-21 US US13/624,085 patent/US20130015114A1/en not_active Abandoned
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2014
- 2014-05-27 US US14/287,373 patent/US9616397B2/en active Active
Non-Patent Citations (1)
Title |
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Also Published As
Publication number | Publication date |
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US9616397B2 (en) | 2017-04-11 |
DE102010003224A1 (de) | 2011-09-29 |
EP2536490B1 (de) | 2014-09-10 |
US20140251886A1 (en) | 2014-09-11 |
US20130015114A1 (en) | 2013-01-17 |
DE102010003224B4 (de) | 2022-11-03 |
WO2011117148A1 (de) | 2011-09-29 |
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