EP2210667A1 - Pipette with tip ejector - Google Patents

Pipette with tip ejector Download PDF

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Publication number
EP2210667A1
EP2210667A1 EP09000662A EP09000662A EP2210667A1 EP 2210667 A1 EP2210667 A1 EP 2210667A1 EP 09000662 A EP09000662 A EP 09000662A EP 09000662 A EP09000662 A EP 09000662A EP 2210667 A1 EP2210667 A1 EP 2210667A1
Authority
EP
European Patent Office
Prior art keywords
stop
counter
device according
dosing device
ring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP09000662A
Other languages
German (de)
French (fr)
Other versions
EP2210667B1 (en
Inventor
Herbert Belgardt
Burkhardt Reichmuth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf AG
Original Assignee
Eppendorf AG
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Filing date
Publication date
Application filed by Eppendorf AG filed Critical Eppendorf AG
Priority to EP09000662.8A priority Critical patent/EP2210667B1/en
Publication of EP2210667A1 publication Critical patent/EP2210667A1/en
Application granted granted Critical
Publication of EP2210667B1 publication Critical patent/EP2210667B1/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/0275Interchangeable or disposable dispensing tips
    • B01L3/0279Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/08Ergonomic or safety aspects of handling devices
    • B01L2200/087Ergonomic aspects

Abstract

The dosing device has a base body (2), where a seat (8) is arranged at the base body for mounting a pipette tip (9) in a detachable manner. A starting device is arranged on the base body for releasing the pipette tip from the seat. A stop or a counter stop is elastically deformable perpendicular to the stop surface.

Description

  • The invention relates to a metering device for quantities of liquid, in particular in the range of 100 nl to several 100 ml, with at least one seat for releasably securing a pipette tip and a discharge device for releasing the pipette tip of the seat.
  • Dosing devices are mainly used in the laboratory for dosing liquids. The liquids are taken up in pipette tips and discharged from it. Pipette tips are small tubes with an opening at the bottom and an opening at the top. The lower opening is usually smaller than the upper opening. By means of a displacement device, liquid is sucked through the lower opening in the pipette tip and ejected. The displacement device is usually a cylinder with a piston displaceable therein. In air cushion systems, the piston and cylinder are integrated into the metering device and communicate with it through the top opening of the pipette tip so that the metering of the fluid is communicated via an air cushion. In direct displacement systems, the piston and cylinder are integrated into the tip and act directly on the aspirated fluid. Such pipette tips are also referred to as syringes.
  • Dosing devices that collect liquid in a single cut and deliver it into a single cut are called pipettes. Dosing device, with which liquid is taken in a single or multiple cuts and dispensed in several sections, referred to as a dispenser.
  • The pipette tip is detachably connected to the dosing device so that it can be exchanged for a fresh pipette tip after use, thereby avoiding contamination at subsequent dosages. Plastic pipette tips for single use are available at low cost.
  • The metering device has at least one seat for releasably securing one or more pipette tips. The seat is often a conical hub on which a pipette tip is clamped to the upper opening. Furthermore, there are metering devices having a bore into which a pipette tip can be clamped with the end having the upper opening. For holding syringes with pistons and cylinders, the dosing device may include receptacles for an upper portion of the cylinder and a piston rod of the piston, and gripping means for holding the upper portions of the cylinder and piston rod in the receptacles. The attachment can be done without touching the pipette tip by placing the metering device with its seat on the pipette tip, which is available in a holder.
  • To avoid contamination of the user, it is desirable over this to solve the used pipette tip without touching the seat. For this metering devices are often equipped with a discharge device for pipette tips. In known embodiments, the discharge device on a discharge bar, which is connected below with an ejector, which is designed for example as a discharge sleeve or as a discharge plate. The ejector is assigned to the upper edge region of a pipette tip attached to the metering device. A coil spring pushes the discharge rod into a starting position in which it bears against an abutment stop, which is firmly connected to the dosing device housing. The discharge bar is connected at the top with a discharge button. By pressing the ejection button can against the action of the coil spring the With its ejector, the ejection device is pressed against the upper edge of the pipette tip or syringe so that it is released from the seat.
  • In known pipettes, e.g. the eject button connected via a snap connection with the discharge rod. The stopper is a snap ring made of steel, which is mounted in an annular groove of the discharge bar. To install the snap ring, the eject button must be pressed so that the lower end of the ejector rod protrudes from the pipette housing. The assembly of the small snap ring by means of a special tool is difficult. If the release button is released during operation, the coil spring presses the ejection device into its starting position so that the snap ring strikes against the counterstop. The counter-stop is formed by the edge of a hole in a rigid housing wall, which is interspersed by the discharge rod. Here, a strong pulse is introduced into the discharge device. So that the ejection button does not accidentally loosen, it is attached by means of a shock-resistant snap on the discharge rod. When disassembling the pipette for maintenance or repair, the ejection button must be drilled to release the snapping with a tool. The drilled ejection button must be replaced by a new ejection button.
  • Based on this, the present invention seeks to provide a metering device with a discharge device available, which is easier to assemble and disassemble.
  • The object is achieved by a metering device with the features of claim 1. Advantageous embodiments of the metering device are specified in the subclaims.
  • The metering device according to the invention has
    • a basic body,
    • at least one seat arranged on the main body for releasably securing a pipette tip,
    • a discharge device arranged on the main body for releasing the pipette tip or syringe from the seat,
      • the ejector movable relative to the seat,
      • a transfer mechanism operatively connected to the ejector for moving the ejector with respect to the seat, drive means for driving the transfer mechanism, and
      • has a stop arranged on the transmission mechanism,
    • a arranged on the main body counter-attack, which is assigned to the stop and limits the movement of the transmission mechanism upwards, and
    • a return means, which acts on the one hand on the base body and on the other hand on the discharge device and presses the stop against the counter-stop,
    • characterized in that the stop and / or counter-stop is elastically deformable perpendicular to its stop surface.
  • The metering device according to the invention has a base body, which is a supporting structure for further components of the metering device. The main body is for example a housing. At least one seat for releasably securing a pipette tip is arranged on the base body. When designed as a single-channel dosing device a single seat is available. When running as Mehrkanaldosiervorrichtung the metering device has several seats, for example, eight. The seat for releasably securing a pipette tip can be designed as a conical approach for attaching a pipette tip or as a hole for insertion of a pipette tip syringe. Further, when the pipette tip is a syringe, the seat may include receptacles for upper portions of the cylinder and piston associated with gripping means for holding the syringe to the housing.
  • On the main body is a discharge device with a relative to the seat movable ejector available. The ejector is for example a discharge sleeve or a discharge plate. A Einkanaldosiervorrichtung example, with a discharge sleeve and a Mehrkanaldosiervorrichtung is provided for example with a discharge plate, the discharge plate is also assigned to several seats. The ejection device has a transmission mechanism operatively connected to the ejector and drive means for driving the transmission mechanism. The ejector can be detachably or non-detachably operatively connected to the transmission mechanism. In particular, ejector and transfer mechanism can be releasably or non-releasably firmly connected to each other, for example, by a clamp connection or by a one-piece design when transmission mechanism and ejector are made as a part of a material. The drive means may be manual drive means or motor drive means such as electric motors. Manual drive means may comprise a release button operatively connected to the transfer mechanism and motor drive means having an electrical linear drive connected to the transfer mechanism. The transmission mechanism is also provided with a stop. On the main body there is a counter-stop, which cooperates with the stop to limit the movement of the ejector upwards, ie from the seat, away. One at base body and the ejector attacking return means presses the ejection device with the stop against the counter-stop when the transmission mechanism is not driven by the drive means. Thus, by operating the drive means, the transfer mechanism is displaced against the action of the return means with the ejector down, ie toward the seat, and the return means displaces the transfer mechanism away from the seat until the stop presses against the counter-stop when the drive means is not actuated.
  • In the conventional pipette, the snap ring and the cooperating housing wall are rigid perpendicular to their stop surfaces, so that the snap ring is abruptly braked when hitting the housing wall. In the dosing device according to the invention, however, the stop and / or the counter-stop is elastically deformable at least in the direction perpendicular to the respective stop surface, so that stop and / or counter-stop deform elastically during the meeting. For this purpose, the stop and / or counter-stop may consist of a material which is elastic in particular perpendicular to the stop surface and / or may be a spring. The invention relates to embodiments in which the stopper is elastic perpendicular to its abutment surface and the counter-abutment is rigid, the counter-stopper is perpendicular to its abutment surface elastic and the abutment is rigid and both the abutment and the abutment stop are perpendicular to their abutment surfaces. The stop and / or counter-stop can be elastic only perpendicular to its stop surface or in the transverse direction or in any direction. The latter is in particular after execution of the stop and / or counter-attack of an elastic material of the case. The perpendicular to the stop surface elastic stop and / or counter-stop can be easily assembled and disassembled. Furthermore, the attack weakens and / or Counter stop from the shock, which occurs after an actuation of the discharge device, when the return means moves the discharge device with the stop against the counter-stop. The shock is not suddenly effective when hitting a hard stop on a hard counter-attack, but delayed by the perpendicular to the stop surface elastic stop and / or counter-stop and thus weakened. Due to this weakening of the shock, a connection between the drive means and the discharge rod can be designed as a detachable latching connection. This is relatively easy to disassemble without being destroyed, so that it is then re-assembled. The weakening of the impulse upon impact of the stop on the counter stop prevents unintentional release of the catch. Through targeted use of force, however, it is possible to separate the locking connection. As a result, the disassembly of the metering device is facilitated in their individual parts, especially during maintenance and repair.
  • Elastic deformable material has damping properties, so it absorbs part of the energy of the impact. The stop and / or counter-stop can be wholly or partly made of a selected elastically deformable material that dampens particularly well. Preferably, the materials used have a resilience according to DIN 53 512, which is less than 25%. Particularly preferred materials have a rebound resilience that is less than 10%. Even more suitable are materials with a resilience of less than 5%. According to one embodiment, the elastically deformable material is rubber-elastic. According to a further embodiment, the stop and / or counter-stop consists of an elastomer (for example rubber or silicone rubber) or a thermoplastic elastomer. According to a further embodiment, the stop and / or counter-stop is made of an autoclavable material. With appropriate design of the other components of the metering device this is a total autoclavable. According to a further embodiment, the stop and / or counter stop is made of a fluoroelastomer (eg Viton® from DuPont), preferably with a resilience of about 6.4%. Particularly temperature-resistant and therefore suitable for autoclaving fluoroelastomers are available. According to a further embodiment are particularly suitable as elastically deformable material: perfluorinated rubber (FFPM), butyl rubber (IIR) and chlorosulfonated polyethylene (CSM).
  • The stop and / or counter-stop can have different geometries. If the stop and / or counter-stop is made of a material which is elastically deformable at least in the direction perpendicular to its abutment surface, the abutment may be formed, for example, by a bolt of the same material passing through a bore of a rod portion of the transfer mechanism and laterally projecting from the rod portion. The stop may also be nubs of the elastic material projecting from the rod section. Further, this may be a laterally projecting portion of the rod portion, which consists entirely of the elastic material. The counter-stop may be a housing wall made entirely of the elastic material with a bore through which a rod portion of the transmission mechanism is passed. According to a preferred embodiment, the stop is a seated on a rod portion of the transmission mechanism ring and / or the counter-stop a rod portion of the transmission mechanism enclosing ring. This embodiment can be realized particularly inexpensive. In addition, the execution of the stop and / or counter-attack as a ring facilitates assembly and disassembly, since the simply axially mounted on a rod portion of the transmission mechanism and removed from this and / or inserted into a bore of a housing and removed from this. The ring can be configured differently. The invention particularly relates to circular cylindrical configurations of the ring. According to a preferred embodiment, the ring is a torus. The toroidal ring has a circular cylindrical cross-section. Pushing on a discharge rod and the removal of the discharge rod is facilitated in a toroidal ring by the relatively low friction. The toroidal ring may in particular be an O-ring that can otherwise be used as a sealing element.
  • The ring is elastically expanded placed on the rod portion so that it is stuck due to its elastic bias on the rod portion.
  • Next, the ring may consist only partially of an elastically deformable material. Thus, the elastically deformable material in a receptacle, for. B. be received from metal, so that the elastically deformable material forms a stop surface at the meeting of stop and counter-attack.
  • According to one embodiment, the ring is seated in an annular groove of the rod portion, wherein the ring protrudes outside over the lateral surface of the rod portion. In the annular groove of the ring is fixed in position on the rod section. The outside in front of the peripheral region of the ring forms a stop surface, which cooperates with the counter-stop. The ring can be arranged without elastic bias in the annular groove. Then it is only elastically expanded when brushing on the rod section until it snaps into the annular groove. Conversely, it is elastically expanded during stripping until it is released from the rod section.
  • According to one embodiment, the ring sits under elastic bias on the rod portion. This embodiment is possible in embodiments in which the ring sits on a smooth rod section. Further, it is possible in embodiments in which the ring is seated in an annular groove or on a rod portion having at least one projection for laterally supporting the ring in a certain position.
  • According to a further embodiment, the ring is arranged in a below the counter-attack from the outside accessible lower end portion of the rod portion having a releasable connection with the ejector, which is preferably guided movably on the base body. The detachable connection of the rod portion with the ejector is for example a plug or clamp connection. It can be made in particular by the fact that the lower end of the rod portion is pressed into a bore of the ejector. At least after releasing the ejector from the rod portion, the lower end portion is accessible from outside, so that the ring can be removed from the rod portion.
  • The transmission mechanism can be executed differently. You can, for example, one of the in the EP 0 992 288 B1 Be described transmission that transmits the axial drive movement of the drive device in an axial movement of the discharge element. According to one embodiment, the transmission mechanism is a discharge rod. The discharge rod may consist of a single rod portion which may be wholly or partially rectilinear and wholly or partially curved and / or bent. Furthermore, the discharge rod may have a plurality of rod sections. The invention relates to embodiments in which the discharge bar has parallel bar sections. Furthermore, the discharge bar with different Be executed cross-sections (eg., Rectangular, circular, cylindrical, hollow cylindrical), wherein along the discharge rod everywhere the same cross-sectional shape can be present with the same or different dimensions or different cross-sectional shapes.
  • According to a further embodiment, the upper end of the transmission mechanism is attached via a releasable latching connection to a drive means. According to one embodiment, the releasable latching connection comprises a latching hook and an undercut for the latching hook, the locking surfaces have an angle of 10 ° to 50 °. Preferably locking angle of 15 ° to 45 ° are used, particularly preferably locking angle of 25 ° to 35 °. Although a latching connection with locking surfaces with these angles holds operational loads, as they occur in the muted impact of the transmission mechanism with the stop on the counter-stop. Under increased use of force, however, this locking connection is solvable. This is especially true in execution of the parts of the locking connection of a plastic. The angle of the locking surfaces refers to their inclination to an axis, in the direction of which the load of the locking connection is made during operation. Optionally, the locking connection can be solved with the aid of a tool.
  • According to a further embodiment, the drive means is a discharge button with a top wall with a standing from the bottom in front of locking hooks with the locking surface at the bottom. According to a further embodiment, the ejection button on the periphery has a diaphragm which laterally covers a free space between the top wall and an upper region of a housing of the metering device. Optionally, the locking connection can be achieved by inserting a strip-shaped tool through a gap between the ejection button and the housing. According to a further embodiment, the ejection button has two dome protruding from the underside of the top wall, each having a bore, and the ejector rod has two pins at the top end which are inserted into the bore of the domes. Together with the locking connection, this causes a solid, non-rotating connection between the push button and the ejection bar. According to one embodiment, the two pins project from an upper traverse of the discharge rod, below the traverse has two substantially parallel bar sections, which are interconnected below by a lower cross member, and protrudes from the bottom of the lower cross member, a lower bar section, the wearing the ring of elastically deformable material. The two parallel bar sections can accommodate, for example, an electric drive motor of an electrically driven metering device between them and thus favor a space-saving design of the metering device.
  • Restoring means are means by which two objects are held in at least one direction in a defined position to each other and ensure that the two objects get back into the same defined position when they were brought by an external influence from the position, as soon as this Influence is no longer present. The return means can be designed differently. According to a preferred embodiment, they are spring means (eg a coil spring sitting on a discharge bar or a gas pressure spring) and / or hydraulic means (eg compressive liquids) and / or pneumatic means and / or magnetically acting means (eg with each other) repelling magnet, one fixed to the housing and the other fixedly connected to the transmission mechanism) and / or electric drive means (eg in an embodiment of the drive means as electric drive means for actuating and resetting the ejector). Used as a return means Federmittel used as a helical spring, so should your spring constant D according to the Hooke's law in particular smaller than 0.2 N / mm, preferably even less than 0.1 N / mm and more preferably less than 0.05 N / mm.
  • According to a further embodiment, the metering device is a manual and / or an electrical metering device. In a manual metering device, a displacer or displacer (eg, a piston in a cylinder) is manually driven. In an electrical metering device, the displacement element is driven by means of an electric drive motor. In an electrical metering device, the dosages can be triggered or stopped manually. A program-controlled operation is also possible. According to a further embodiment, the dosing device is a stationary dosing device and / or a manual dosing device.
  • According to a further embodiment, the metering device is a pipette and / or a dispenser. It can be designed as a pipette with a constant or adjustable dosing volume or as a dispenser with a constant or adjustable dispensing volume. Also combinations of pipette and dispenser are possible. A preferred embodiment is a manually or electrically driven handheld pipette or hand dispenser.
  • Finally, the invention relates to a method for mounting a discharge device on a metering device, in which a transmission mechanism of the discharge device is inserted into a housing of the metering device, so that a rod portion of the transfer mechanism protrudes from a hole of the housing and a ring, which is an elastically deformable material has, is pushed onto the protruding rod portion. The ring may consist partially or entirely of the elastically deformable material. Preferably, the ring is in pushed an annular groove formed on the rod portion. The sliding of the ring is possible to simplify the production step without consuming special tools.
  • Preferably, before or after the sliding of the ring at the upper end of the transmission mechanism, a drive means with a releasable connection, in particular latching connection attached. In particular, the latching connection comprises a latching hook and an undercut for the latching hook.
  • The invention will be explained in more detail with reference to the accompanying drawings of exemplary embodiments. In the drawings show.
  • Fig. 1
    an electric pipette, driven by an electric drive motor with a pipette tip equipped in a side view, in which the housing has been removed in the region of the ejection device;
    Fig. 2
    a manual pipette equipped with a pipette tip, in a side view, in which the housing was removed in the area of the actual pipette;
    Fig. 3
    a discharge rod with a discharge button of the electric pipette of FIG. 1 in a perspective view obliquely from the side;
    Fig. 4
    a discharge button separated from the top of the discharge bar in an enlarged perspective view obliquely from the side;
    Fig. 5
    the upper portion of the same discharge bar in a perspective view obliquely from the opposite side;
    Fig. 6
    lower end of the upper housing part of a pipette with the lower end of the discharge rod in non-actuated discharge device in an enlarged longitudinal section;
    Fig. 7
    lower end of the upper housing part of a pipette with non-actuated ejection device in a perspective view obliquely from below and from the side;
    Fig. 8
    lower end of the upper housing part of a pipette with the lower end of the discharge bar with actuated discharge device in an enlarged longitudinal section;
    Fig. 9
    lower end of the upper housing part of a pipette with actuated ejection device in a perspective view obliquely from below and from the side.
  • In the present application, the terms "top" and "bottom" and "top" and "bottom" and "bottom end" and "top" refer to the preferred position of the pipette in operation, in which the pipette is vertically aligned and a Pipette tip connected to the pipette is arranged below the pipette.
  • In Fig. 1 an electrical pipette is shown in side view, which is driven by an electric drive motor. It was in Fig. 1 around
  • Discard the pipette the housing omitted to represent the ejector.
    In Fig. 2 a manual pipette is shown in side view, which can be operated by hand. It was in Fig. 2 in the area of the actual pipette, ie where the actual pipetting takes place, the housing omitted to show the essential components of a pipette.
  • According to Fig. 1 and 2 has a pipette according to the invention 1 designed as a handle pipette housing 2 with a housing upper part 3 and a lower housing part 4. The upper housing part 3 has below bottom connecting means 5 and the lower housing part 4 has top connecting means 6, through the upper housing part 3 and lower housing part 4 are interconnected. The connecting means 5, 6 are designed, for example, as a screw or cancelable latching connection.
  • Below the upper connecting means 6, the lower housing part 4 has a housing shaft 7, which has at the lower end a seat 8 in the form of a conical Aufsteckzapfens.
  • A pipette tip 9 made of plastic has a tubular body 10 with an upper opening 11 and a lower opening 12. The body 10 is conically designed with a small cone angle. The pipette tip 9 is attached to the upper opening 11 on the seat 8, so that it clamps, but can be pushed off the seat 8.
  • Above the manual pipette protrudes from the upper housing part 3, an actuating knob 13 which is fixedly connected to a lifting rod 14, which is extends to the lower end of the upper housing part 3. The lifting rod 14 carries on the circumference of an annular bead 15th
  • Furthermore, an adjusting sleeve 16 protrudes upwards out of the upper housing part for setting the metering quantity, which surrounds the actuating knob 13. The adjusting sleeve 16 is connected via grooves on the inner circumference and engaging therein driver via a gear rotatably and axially relatively movable with a spindle 18 for adjusting the stroke of the lifting rod 14. The spindle 18 has a contact surface 19 for the annular bead 15, which is axially displaceable to the lifting rod 14 to limit their stroke.
  • In the lower housing part 4, a piston 21 is slidably disposed in a cylinder 22. The piston 21 is connected via a piston rod 23 with a pressure plate 24. A piston return spring 25, which is supported one end in the lower housing part 4 and other ends engages the pressure plate 24, presses the pressure plate 24 against the lower end of the lifting rod fourteenth
  • The cylinder 22 is connected via a channel 26 to an opening 27 in the lower end of the seat 8.
  • Further, on the pipette housing 2 of the pipette 1 of Fig. 1 and 2 a discharge device 28 is arranged. This has in the upper housing part 3 a discharge bar 29, which is connected at the top with a discharge button 30.
  • According to Fig. 3 to 5 the discharge rod 29 has two substantially parallel bar sections 29.1, 29.2, which are connected to each other at the top by an upper cross member 31.1 and below by a lower cross member 31.2. From the upper cross member 31.1 are two pins 32.1, 32.2 high, which are parallel to the extend two rod sections 29.1, 29.2. Furthermore, the upper cross-member 31.1 has an undercut 33 with a detent surface 34 which is directed approximately at 30 ° to the axes of the rod sections 29.1, 29.2.
  • From the lower cross member is parallel to the rod sections 29.1, 29.2, a lower rod section 29.3 before. This has in the vicinity of the lower end an annular groove 35. Under the annular groove 35, a further circumferential groove 36 is present.
  • The ejection button 30 has a top wall 37 with a locking hook 38 projecting from the underside, which has a further latching surface 39 at the lower end. The locking surface 39 has an angle with respect to a perpendicular to the latching hook of about 30 °, which corresponds to the angle of the undercut 33 of the discharge rod 29.
  • Parallel to the latching hook 38 are two dome 40.1, 40.2, each with a further bore 41.1, 41.2 from the top wall. Furthermore, the ejection button 30 has an aperture 42 at an edge portion of its top wall 37.
  • According to Fig. 3 the discharge button 30 with the other holes 41.1, 41.2 of the dome 40.1, 40.2 pushed onto the pins 32.1, 32.2 of the discharge rod 29 and latched to the locking surface 39 of the latching hook 38 with the locking surface 34 of the undercut 33. This locking connection is due to the angle of the locking surfaces 34, 39 with the use of an increased, ejection button 30 and discharge rod 29 pulling apart force, resolvable.
  • According to Fig. 1 and 2 the discharge rod 29 is arranged with the parallel rod sections 29.1, 29.2 in a guide channel 43 of the upper housing part 3. The ejection button 30 is locked with the discharge rod 29. The lower bar section 29.3 is passed through a hole 44 in a cross section of the upper housing part 3 partially obstructing bottom wall 45.
  • On the lower rod portion 29.3 is a coil spring 46, e.g. with a spring constant D of 0.13 N / mm out, which is supported one end on the lower cross member 31.2 and other ends on the bottom wall 45.
  • In the annular groove 35 of the lower rod part 29.3 sits a toroidal ring 47 made of an elastically deformable material, such as. Viton® from DuPont, in particular with a rebound resilience of 6.4%. The ring 47 protrudes slightly beyond the circumference of the rod part 29.3. On the housing shaft 7, a discharge sleeve 48 is guided, which has a lateral projection 49 at the top, which has a further bore 50.
  • According to Fig. 1 and 2 the lower end of the discharge rod 29 is pressed into the further bore 50. The lower edge 51 of the discharge sleeve 48 is assigned to the seat 8.
  • In the position according to Fig. 2 the piston 21 is maximally pushed out of the cylinder 22 by the piston return spring 25 and the release sleeve 48 is displaced away from the seat 8 by the coil spring 46 to the maximum extent. In this initial position of the ring 47 abuts against the lower bottom of the bottom wall 45, which forms a counter-stop 52 (see. Fig. 6 and 7 ). Furthermore, the pressure plate 24 is in contact with the bottom of the lifting rod 14.
  • By adjusting the adjusting sleeve 16, a metered amount can be selected. By actuating the actuating knob 13, air is forced out of the cylinder 22 and the pipette tip 9. Then, the pipette tip 9 can be immersed with the lower opening 12 in a liquid. After releasing the Actuator knob 13 pushes the piston return spring 25, the piston 22 in the starting position and liquid is sucked into the pipette tip 9.
  • The pipette 1 can be moved with the pipette tip 9 via a receptacle and by pressing the actuating button 13 again a selected amount of liquid from the pipette tip 9 are ejected. Subsequently, the operation knob 13 is relieved and the piston 22 returns to its original position.
  • The used pipette tip 9 is released by pressing the ejection button 30 against the force of the coil spring 46 from the seat 8. Here, the discharge sleeve 48 is displaced along the housing shaft 7 down and pushes with its lower edge 51 against the upper edge 11 of the pipette tip 9, so that it is released from the seat 8. In this case, the stop or ring 47 moves away from the counter-stop 52 (cf. Fig. 8 and 9 ). Finally, the discharge button 30 is released and the discharge device 28 returns to its original position. In this case, the ring 47 beats against the counter-stop 52 due to the action of the coil spring 46, wherein the pulse is damped by the elastically deformable material.
  • For disassembly of the ring 47 can be easily deducted from the lower end of the discharge bar 29.3. Further, it is possible to deduct the ejection button 30 against the action of the latching connection 34, 39 under increased effort of the discharge rod 29.

Claims (15)

  1. Dosing device with
    a base body (2),
    at least one seat (8) arranged on the base body (2) for releasably securing a pipette tip (9),
    a discharge device (28) arranged on the main body (2) for releasing the pipette tip (9) from the seat (8),
    - a relative to the seat (8) movable ejector (48),
    a transmission mechanism (29) operatively connected to the ejector (48) for moving the ejector (48) with respect to the seat (8),
    - Drive means (30) for driving the transmission mechanism (29) and
    a stop (47) on the transmission mechanism (29),
    - One on the main body (2) arranged counter-stop (52) which is associated with the stop (47) and limits the movement of the transmission mechanism (29) upwards, and
    - A return means (46), on the one hand on the base body (2) and on the other hand on the discharge device (28) engages and pushes the stop (47) against the counter-stop (52),
    - characterized in that the stop (47) and / or the counter-stop (52) is elastically deformable perpendicular to its abutment surface.
  2. Dosing device according to claim 1, wherein the stop (47) and / or counter-stop (52) comprises an elastically deformable material whose rebound resilience is less than 25%.
  3. Dosing device according to claim 1 or 2, wherein the stop (47) and / or the counter-stop at least partially made of an elastomer or a thermoplastic elastomer and / or a spring.
  4. Dosing device according to one of claims 1 to 3, wherein the stop (47) and / or the counter-stop (52) consists of autoclavable material.
  5. Dosing device according to one of claims 1 to 4, wherein the stop (47) and / or the counter-stop (52) is made of a fluoroelastomer.
  6. Dosing device according to one of claims 1 to 5, wherein the stop (47) on a rod portion (29.3) of the transmission mechanism (29) seated ring and / or the counter-stop is a rod portion (29.3) of the transmission mechanism (29) enclosing ring.
  7. Dosing device according to claim 6, wherein the ring (47) is a torus.
  8. Dosing device according to claim 6 or 7, wherein the ring (47) in an annular groove (35) of the rod portion (29.3) of the transmission mechanism (29) is seated, wherein the ring (47) on the outside over the lateral surface of the rod portion (29.3) projects.
  9. Dosing device according to one of claims 6 to 8, wherein the ring (47) under elastic bias on the rod portion (29.3) sits.
  10. Dosing device according to one of claims 6 to 9, wherein the ring (47) is arranged in a below the counter-stop from the outside accessible lower end portion of the rod portion (29.3) having a releasable connection with the ejector (48).
  11. Dosing device according to one of claims 1 to 9, wherein the ejector (48) and the transmission mechanism (29) are designed as a single part.
  12. Dosing device according to one of claims 1 to 11, wherein the transmission mechanism is a discharge rod (29).
  13. Dosing device according to one of claims 1 to 12, wherein the upper end of the transmission mechanism (29) via a latching connection (34, 39) to a drive means (30) is fixed, which has a latching hook (38) and an undercut (33) for the Latch hook (38) includes.
  14. Dosing device according to one of claims 1 to 13, wherein the return means comprises spring means (46) and / or hydraulic means and / or pneumatic means and / or magnetically acting means and / or electrical drive means.
  15. Method for mounting a discharge device (28) on a metering device (1), in which a transfer mechanism (29) of the discharge device in a housing (2) of the metering device (1) is inserted, so that a rod portion (29.3) of the transfer mechanism (29) a hole (44) of the housing (2) protrudes and an elastically deformable ring (47) on the protruding rod portion (29.3) is pushed.
EP09000662.8A 2009-01-19 2009-01-19 Metering device with tip ejector Active EP2210667B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP09000662.8A EP2210667B1 (en) 2009-01-19 2009-01-19 Metering device with tip ejector

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP09000662.8A EP2210667B1 (en) 2009-01-19 2009-01-19 Metering device with tip ejector
CN201010005412.6A CN101819054B (en) 2009-01-19 2010-01-19 Dosing device

Publications (2)

Publication Number Publication Date
EP2210667A1 true EP2210667A1 (en) 2010-07-28
EP2210667B1 EP2210667B1 (en) 2019-05-08

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EP09000662.8A Active EP2210667B1 (en) 2009-01-19 2009-01-19 Metering device with tip ejector

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EP (1) EP2210667B1 (en)
CN (1) CN101819054B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102698824A (en) * 2012-06-07 2012-10-03 林昂 Novel liquid transfer device
DE102012209314A1 (en) * 2012-06-01 2013-12-05 Albert-Ludwigs-Universität Freiburg Device and method for dispensing or receiving a liquid volume

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Publication number Priority date Publication date Assignee Title
US5104624A (en) * 1989-10-20 1992-04-14 Costar Corporation Pipetter
WO1996037302A1 (en) * 1995-05-26 1996-11-28 Rainin Instrument Co., Inc. Pipette tip ejector
WO2001057490A1 (en) * 2000-02-03 2001-08-09 Rainin Instrument Llc. Pipette device with tip ejector utilizing stored energy
EP1557222A2 (en) * 2004-01-21 2005-07-27 Eppendorf Ag Pipette with pipette tip ejector
EP0992288B1 (en) 1998-10-06 2007-03-07 Eppendorf Ag Pipette tip ejection arrangement

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006024051A1 (en) * 2006-05-23 2007-12-06 Eppendorf Ag Electronic dosing device for dosing liquids
DE102006037213A1 (en) * 2006-08-09 2008-02-14 Eppendorf Ag Electronic dosing device for dosing liquids

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5104624A (en) * 1989-10-20 1992-04-14 Costar Corporation Pipetter
WO1996037302A1 (en) * 1995-05-26 1996-11-28 Rainin Instrument Co., Inc. Pipette tip ejector
EP0992288B1 (en) 1998-10-06 2007-03-07 Eppendorf Ag Pipette tip ejection arrangement
WO2001057490A1 (en) * 2000-02-03 2001-08-09 Rainin Instrument Llc. Pipette device with tip ejector utilizing stored energy
EP1557222A2 (en) * 2004-01-21 2005-07-27 Eppendorf Ag Pipette with pipette tip ejector

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012209314A1 (en) * 2012-06-01 2013-12-05 Albert-Ludwigs-Universität Freiburg Device and method for dispensing or receiving a liquid volume
DE102012209314B4 (en) * 2012-06-01 2015-04-02 Albert-Ludwigs-Universität Freiburg Device and method for dispensing or receiving a liquid volume
US9459128B2 (en) 2012-06-01 2016-10-04 Hahn-Schickard-Gesellschaft Fuer Angewandte Forschung E.V. Device and method for dispensing or receiving a liquid volume
CN102698824A (en) * 2012-06-07 2012-10-03 林昂 Novel liquid transfer device

Also Published As

Publication number Publication date
EP2210667B1 (en) 2019-05-08
CN101819054B (en) 2015-07-01
CN101819054A (en) 2010-09-01

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