EP2084285A4 - Co-expression of proline hydroxylases to facilitate hyp-glycosylation of proteins expressed and secreted in plant cells - Google Patents

Co-expression of proline hydroxylases to facilitate hyp-glycosylation of proteins expressed and secreted in plant cells

Info

Publication number
EP2084285A4
EP2084285A4 EP07799434A EP07799434A EP2084285A4 EP 2084285 A4 EP2084285 A4 EP 2084285A4 EP 07799434 A EP07799434 A EP 07799434A EP 07799434 A EP07799434 A EP 07799434A EP 2084285 A4 EP2084285 A4 EP 2084285A4
Authority
EP
European Patent Office
Prior art keywords
hyp
glycosylation
secreted
expression
facilitate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP07799434A
Other languages
German (de)
French (fr)
Other versions
EP2084285A2 (en
Inventor
Marcia Kieliszewski
Jianfeng Xu
Iver P Cooper
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ohio University
Ohio State University
Original Assignee
Ohio University
Ohio State University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ohio University, Ohio State University filed Critical Ohio University
Publication of EP2084285A2 publication Critical patent/EP2084285A2/en
Publication of EP2084285A4 publication Critical patent/EP2084285A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
EP07799434A 2006-07-10 2007-07-10 Co-expression of proline hydroxylases to facilitate hyp-glycosylation of proteins expressed and secreted in plant cells Withdrawn EP2084285A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81955706P 2006-07-10 2006-07-10
PCT/US2007/073137 WO2008008766A2 (en) 2006-07-10 2007-07-10 Co-expression of proline hydroxylases to facilitate hyp-glycosylation of proteins expressed and secreted in plant cells

Publications (2)

Publication Number Publication Date
EP2084285A2 EP2084285A2 (en) 2009-08-05
EP2084285A4 true EP2084285A4 (en) 2010-01-13

Family

ID=38924091

Family Applications (1)

Application Number Title Priority Date Filing Date
EP07799434A Withdrawn EP2084285A4 (en) 2006-07-10 2007-07-10 Co-expression of proline hydroxylases to facilitate hyp-glycosylation of proteins expressed and secreted in plant cells

Country Status (2)

Country Link
EP (1) EP2084285A4 (en)
WO (1) WO2008008766A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6639050B1 (en) 1997-07-21 2003-10-28 Ohio University Synthetic genes for plant gums and other hydroxyproline-rich glycoproteins
US7378506B2 (en) 1997-07-21 2008-05-27 Ohio University Synthetic genes for plant gums and other hydroxyproline-rich glycoproteins
US20060148680A1 (en) 2004-01-14 2006-07-06 Kieliszewski Marcia J Glycoproteins produced in plants and methods of their use
WO2005110015A2 (en) 2004-04-19 2005-11-24 Ohio University Cross-linkable glycoproteins and methods of making the same
EP2448958A4 (en) * 2009-06-01 2013-11-06 Kenneth James Friel Human milk peptides
US8815806B2 (en) 2009-10-28 2014-08-26 University Of Manitoba Yellow pea seed protein-derived peptides
EP2844750B1 (en) * 2012-05-03 2018-06-20 DSM IP Assets B.V. Improved enzyme variants
CN109022464A (en) * 2018-07-02 2018-12-18 西安巨子生物基因技术股份有限公司 The hydroxylacion method of recombination human source collagen type

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006035442A2 (en) * 2004-09-29 2006-04-06 Collplant Ltd. Collagen producing plants and methods of generating and using same

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060148680A1 (en) * 2004-01-14 2006-07-06 Kieliszewski Marcia J Glycoproteins produced in plants and methods of their use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006035442A2 (en) * 2004-09-29 2006-04-06 Collplant Ltd. Collagen producing plants and methods of generating and using same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BERRA EDURNE ET AL: "The hypoxia-inducible-factor hydroxylases bring fresh air into hypoxia signalling", EMBO REPORTS, vol. 7, no. 1, January 2006 (2006-01-01), pages 41 - 45, XP002558270, ISSN: 1469-221X *
MERLE C ET AL: "Hydroxylated human homotrimeric collagen I in Agrobacterium tumefaciens-mediated transient expression and in transgenic tobacco plant", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 515, no. 1-3, 27 March 2002 (2002-03-27), pages 114 - 118, XP004347752, ISSN: 0014-5793 *

Also Published As

Publication number Publication date
WO2008008766A2 (en) 2008-01-17
EP2084285A2 (en) 2009-08-05
WO2008008766A3 (en) 2008-11-20

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