EP2078206A1 - Process for providing a plant with an identification label - Google Patents

Process for providing a plant with an identification label

Info

Publication number
EP2078206A1
EP2078206A1 EP07834641A EP07834641A EP2078206A1 EP 2078206 A1 EP2078206 A1 EP 2078206A1 EP 07834641 A EP07834641 A EP 07834641A EP 07834641 A EP07834641 A EP 07834641A EP 2078206 A1 EP2078206 A1 EP 2078206A1
Authority
EP
European Patent Office
Prior art keywords
tracer molecules
seed
plant
tracer
molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP07834641A
Other languages
German (de)
French (fr)
Inventor
Albert Van Duijn
Arie Draaijer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Original Assignee
Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO filed Critical Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Priority to EP07834641A priority Critical patent/EP2078206A1/en
Publication of EP2078206A1 publication Critical patent/EP2078206A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

Definitions

  • the invention relates to a process for providing a plant with an identification label, seed for cultivating plants which seed comprises tracer molecules, a process for preparing said seed, and the use of tracer molecules for identifying a particular plant among one or more other type of plants.
  • tracer molecules can be used to identify plants and/or plant parts.
  • the plants and/or plant parts are contacted with the tracer molecules which are either taken up inside the plant and/or plant parts or on the surface thereof. In case the plant parts are in the form of a seed, the tracer molecules will remain on the outside of the seed. In this way, the origin, time of production or the owner of plant material can be identified. Moreover, tracking and tracing of plant material throughout the production process facilities a better quality control.
  • Object of the present invention is to provide a less time-consuming and labour-intensive process which allows easy identification of plants.
  • the present invention relates to a process for providing a plant with an identification label, which process comprises cultivating the plant from seed which comprises tracer molecules, thereby allowing the tracer molecules to be incorporated in the plant, whereby the incorporated tracer molecules generate and/or form the identification label.
  • the tracer molecules can be attached to the surface of the seed or the tracer molecules can be incorporated in the seed.
  • the tracer molecules are incorporated in the seed to be used in accordance with the present invention.
  • the tracer molecules are preferably fluorescent tracer molecules.
  • the tracer molecules are selected from the group consisting of vitamins, food grade dyes, laser dyes, textile dyes and pH indicators.
  • Suitable examples of vitamines include Vitamin B and Vitamin A.
  • Suitable examples of food grade dyes include Vitamin B Phosphate and Carotene.
  • Suitable examples of laser dyes include Rhodamine B and Sulfo Rhodamine G.
  • Suitable examples of textile dyes include Basic Red and Basic Yellow.
  • Suitable examples of pH indicators include Porphyrines and HPTS.
  • the tracer molecules are selected from the group consisting of vitamins and food grade dyes.
  • the tracer molecules to be used in accordance with the present invention are preferably non-toxic and non-phytotoxic.
  • the seed to be applied in accordance with the invention and which comprises the tracer molecules can suitably be obtained by applying the tracer molecules onto the surface of seed particles.
  • Any known method can be used to apply the tracer molecules onto the surface of the seed particles or to incorporate the tracer molecules in the seed particles. Suitable techniques include seed coating techniques and seed pelleting techniques.
  • the seed particles can be brought into contact with water which contains the tracer molecules during a priming or steeping step.
  • use can be made of capsules that encapsulate the seed particles and the tracer molecules.
  • the tracer molecules can first be applied onto the surface of the seed particles, whereafter the tracer molecules can be incorporated in the seed particles once the seed particles start to take up water, for instance during a priming or steeping step.
  • the seed which comprises the tracer molecules is obtained by adding the tracer molecules to a coating or pellet which is applied onto the seed particles.
  • the coating is formed by spray-drying a solution which comprises the tracer molecules on the surface of the seed particles or the coating is formed by impregnating the seed particles with a solution which comprises the tracer molecules.
  • the tracer molecules are applied onto the surface of the seed particles by means of a coating which is formed by spray-drying a solution which comprises the tracer molecules on the surface of the seed particles.
  • the coating to be used in accordance with the present invention can be of any type commonly used in the seed industry. Suitable examples include mixtures based on different water based non-phytotoxic polymer structures.
  • pellets to be used in accordance with the present invention can be of any type commonly used in the seed industry. Suitable examples include mixtures with different clay types, fibres from wood flour or peat, fillers such as different kinds of diatomaceous earth and other inert materials with a specific character.
  • the present invention also relates to seed for cultivating plants which seed comprises tracer molecules.
  • the present invention provides a process or seed according to the present invention wherein the plant is selected from plant species that are individually planted in field applications. Suitable examples of such plant species include corn, sugar beet, cotton, onion and most vegetable crops.
  • the amount of tracer molecules should be sufficient to make plant identification possible.
  • the actual amounts to be used will depend on seed particle size, uptake rate of the tracer molecules by the plant, and the size of the plant in question.
  • the amount of tracer molecules will suitably be in the range of from 0.1 to 10 mg per pelleted seed.
  • the amount of tracer molecules in respect of said plant species will be in the range of from 0.1 to 2 mg per pelleted seed.
  • the present invention also relates to the use of tracer molecules for identifying a particular plant among one or more other type of plants, whereby the tracer molecules, present in the plant, originate from the seed of the particular plant.
  • the present invention will now be illustrated by means of the following
  • Figures 1-3 show the result of a test with pelleted Onion seeds.
  • the pellet was provided with 0 (bianco), 0.5 and 2 mg Vitamin B phosphate per seed.
  • the dye was simply mixed with the pelleting material.
  • the pelleted seeds were put into a standard mineral substrate growing medium, and they were provided with normal amounts of water to which standard nutrition solutions were added.
  • the difference between the plantlets originating from the differently treated seeds is clearly visible from pictures taken with a blue excitation source (470-490 nm) which illuminated the plantlets, and detection of the emitted fluorescence was allowed with a camera in the green part of the visible spectrum (520-550 nm).
  • Figure 1 taken from plantlets which were not treated, barely any plantlet is visible.
  • Rhodamine B in beet plants The photograph shown in Figure 4 shows the effect of growing seeds in a growing medium that is provided with different Rhodamine B concentrations. This experiment was done by taking plantlets that were not treated and putting them in a growing solution provided with Rhodamine B to show the uptake of the dye through the roots of the plant. From left to right the Rhodamine B concentrations were 0.01, 0.001, 0.0001, mg/ml water, whereas the right hand plantlet was in a medium without dye (i.e. a bianco experiment). Pictures of the fluorescence from the dye in the plants were taken by illuminating them with green light of a wavelength between 530 nm and 560 nm.
  • the fluorescence was detected with a camera provided with a long pass filter, passing all wavelengths longer than 580 nm. There is a clearly visible difference between the plants growing in the medium which were provided with two highest concentrations when compared with the low concentration and the bianco experiment. The yellowish fluorescence is brightest in the left plantlet and is the least in the two plantlets on the right hand side
  • the present invention provides a very attractive process for providing a plant with a label that clearly allows the plant to be identified amongst other plants.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention provides a process for providing a plant with an identification label, which process comprises cultivating the plant from seed which comprises tracer molecules, thereby allowing the tracer molecules to be incorporated in the plant, whereby the incorporated tracer molecules generate and/or form the identification label. The invention further relates to seed for cultivating plants which seed comprises tracer molecules, a process for preparing seed, and the use of tracer molecules for identifying a particular plant among one or more other type of plants.

Description

Title: PROCESS FOR PROVIDING A PLANT WITH AN IDENTIFICATION LABEL
The invention relates to a process for providing a plant with an identification label, seed for cultivating plants which seed comprises tracer molecules, a process for preparing said seed, and the use of tracer molecules for identifying a particular plant among one or more other type of plants. It is known from WO 2004/065945 that tracer molecules can be used to identify plants and/or plant parts. The plants and/or plant parts are contacted with the tracer molecules which are either taken up inside the plant and/or plant parts or on the surface thereof. In case the plant parts are in the form of a seed, the tracer molecules will remain on the outside of the seed. In this way, the origin, time of production or the owner of plant material can be identified. Moreover, tracking and tracing of plant material throughout the production process facilities a better quality control. Further, it allows the identification of batches of plant material that have received special treatments or have a specific property. An essential drawback of such known application is that the plants and/or plant parts to be treated with the tracer molecules need as such, of course, to be identified first before the tracer molecules can be brought into contact with the plants and/or plant parts concerned. In other words, a preselection of the plants needs to be carried out before the tracer molecules can actually be used. Evidently, this makes such an application a time-consuming and labour-intensive exercise.
Object of the present invention is to provide a less time-consuming and labour-intensive process which allows easy identification of plants.
Surprisingly, it has been found that this can be realised when use is made of seed that comprises tracer molecules. Accordingly, the present invention relates to a process for providing a plant with an identification label, which process comprises cultivating the plant from seed which comprises tracer molecules, thereby allowing the tracer molecules to be incorporated in the plant, whereby the incorporated tracer molecules generate and/or form the identification label.
Suitably, the tracer molecules can be attached to the surface of the seed or the tracer molecules can be incorporated in the seed. Preferably, the tracer molecules are incorporated in the seed to be used in accordance with the present invention.
In the process according to the invention, the tracer molecules are preferably fluorescent tracer molecules.
Suitably, the tracer molecules are selected from the group consisting of vitamins, food grade dyes, laser dyes, textile dyes and pH indicators.
Suitable examples of vitamines include Vitamin B and Vitamin A. Suitable examples of food grade dyes include Vitamin B Phosphate and Carotene.
Suitable examples of laser dyes include Rhodamine B and Sulfo Rhodamine G.
Suitable examples of textile dyes include Basic Red and Basic Yellow. Suitable examples of pH indicators include Porphyrines and HPTS. Preferably, the tracer molecules are selected from the group consisting of vitamins and food grade dyes. The tracer molecules to be used in accordance with the present invention are preferably non-toxic and non-phytotoxic.
The seed to be applied in accordance with the invention and which comprises the tracer molecules can suitably be obtained by applying the tracer molecules onto the surface of seed particles. Any known method can be used to apply the tracer molecules onto the surface of the seed particles or to incorporate the tracer molecules in the seed particles. Suitable techniques include seed coating techniques and seed pelleting techniques. In other suitable embodiments, the seed particles can be brought into contact with water which contains the tracer molecules during a priming or steeping step. In yet another embodiment, use can be made of capsules that encapsulate the seed particles and the tracer molecules. The tracer molecules can first be applied onto the surface of the seed particles, whereafter the tracer molecules can be incorporated in the seed particles once the seed particles start to take up water, for instance during a priming or steeping step.
Preferably, the seed which comprises the tracer molecules is obtained by adding the tracer molecules to a coating or pellet which is applied onto the seed particles.
Preferably, the coating is formed by spray-drying a solution which comprises the tracer molecules on the surface of the seed particles or the coating is formed by impregnating the seed particles with a solution which comprises the tracer molecules.
More preferably, the tracer molecules are applied onto the surface of the seed particles by means of a coating which is formed by spray-drying a solution which comprises the tracer molecules on the surface of the seed particles.
The coating to be used in accordance with the present invention can be of any type commonly used in the seed industry. Suitable examples include mixtures based on different water based non-phytotoxic polymer structures.
The pellets to be used in accordance with the present invention can be of any type commonly used in the seed industry. Suitable examples include mixtures with different clay types, fibres from wood flour or peat, fillers such as different kinds of diatomaceous earth and other inert materials with a specific character.
The spray-dry and impregnation processes to be used for applying the coating on the seed particles will be carried out under conditions well understood by the skilled person in the art.
The present invention also relates to seed for cultivating plants which seed comprises tracer molecules.
Further, the present invention provides a process or seed according to the present invention wherein the plant is selected from plant species that are individually planted in field applications. Suitable examples of such plant species include corn, sugar beet, cotton, onion and most vegetable crops.
The amount of tracer molecules should be sufficient to make plant identification possible. The actual amounts to be used will depend on seed particle size, uptake rate of the tracer molecules by the plant, and the size of the plant in question.
In case of sugar beet, corn, cotton or onion the amount of tracer molecules will suitably be in the range of from 0.1 to 10 mg per pelleted seed. Preferably, the amount of tracer molecules in respect of said plant species will be in the range of from 0.1 to 2 mg per pelleted seed.
The present invention also relates to the use of tracer molecules for identifying a particular plant among one or more other type of plants, whereby the tracer molecules, present in the plant, originate from the seed of the particular plant. The present invention will now be illustrated by means of the following
Examples.
Examples
Vitamin B phosphate in Onion seeds
Figures 1-3 show the result of a test with pelleted Onion seeds. The pellet was provided with 0 (bianco), 0.5 and 2 mg Vitamin B phosphate per seed. The dye was simply mixed with the pelleting material. Next, the pelleted seeds were put into a standard mineral substrate growing medium, and they were provided with normal amounts of water to which standard nutrition solutions were added. The difference between the plantlets originating from the differently treated seeds is clearly visible from pictures taken with a blue excitation source (470-490 nm) which illuminated the plantlets, and detection of the emitted fluorescence was allowed with a camera in the green part of the visible spectrum (520-550 nm). In the left photograph (Figure 1) taken from plantlets which were not treated, barely any plantlet is visible. The two right hand photographs (Figures 2 and 3) clearly show the effect of adding vitamin B phosphate to the pellet. The small difference in intensity between the fluorescence detected from both plants treated with the fluorescent tracer shows that the dye uptake by the plant was probably saturated en that lower doses are possible.
Rhodamine B in beet plants The photograph shown in Figure 4 shows the effect of growing seeds in a growing medium that is provided with different Rhodamine B concentrations. This experiment was done by taking plantlets that were not treated and putting them in a growing solution provided with Rhodamine B to show the uptake of the dye through the roots of the plant. From left to right the Rhodamine B concentrations were 0.01, 0.001, 0.0001, mg/ml water, whereas the right hand plantlet was in a medium without dye (i.e. a bianco experiment). Pictures of the fluorescence from the dye in the plants were taken by illuminating them with green light of a wavelength between 530 nm and 560 nm. The fluorescence was detected with a camera provided with a long pass filter, passing all wavelengths longer than 580 nm. There is a clearly visible difference between the plants growing in the medium which were provided with two highest concentrations when compared with the low concentration and the bianco experiment. The yellowish fluorescence is brightest in the left plantlet and is the least in the two plantlets on the right hand side
From the above experiments it will be clear that the present invention provides a very attractive process for providing a plant with a label that clearly allows the plant to be identified amongst other plants.

Claims

Claims
1. A process for providing a plant with an identification label, which process comprises cultivating the plant from seed which comprises tracer molecules, thereby allowing the tracer molecules to be incorporated in the plant, whereby the incorporated tracer molecules generate and/or form the identification label.
2. The process according to claim 1, wherein the tracer molecules are fluorescent tracer molecules.
3. The process according to claim 2, wherein the tracer molecules are selected from the group consisting of vitamins, food grade dyes, laser dyes, textile dyes and pH indicators.
4. The process according to claim 3, wherein the tracer molecules are selected from the group consisting of vitamins and food grade dyes.
5. The process according to claim 3 or 4, wherein the tracer molecules are non-toxic and non-phytotoxic.
6. The process according to any one of claims 1-5, wherein the seed which comprises the tracer molecules is obtained by applying the tracer molecules onto the surface of seed particles.
7. The process according to claim 6, wherein a coating or pellet is applied on the seed particles which coating or pellet comprises the tracer molecules.
8. The process according to claim 7, wherein the coating is formed by spray-drying a solution which comprises the tracer molecules on the surface of the seed particles or the coating is formed by impregnating the seed particles with a solution which comprises the tracer molecules.
9. The process according to claim 8, wherein the coating is formed by spray-drying a solution which comprises the tracer molecules on the surface of the seed particles.
10. The process according to any one of claims 1-6, wherein the tracer molecules are incorporated in the seed.
11. Seed for cultivating plants which seed comprises tracer molecules.
12. Seed according to claim 11, wherein the tracer molecules are incorporated in the seed.
13. A process or seed according to any one of claims 1-12, wherein the plant is selected from plant species that are individually planted in field applications.
14. A process for preparing seed as defined in claim 11 or 12 comprising spray-drying a solution comprising tracer molecules on the surface of seed particles or impregnating seed particles with a solution comprising tracer molecules.
15. Use of tracer molecules for identifying a particular plant among one or more other type of plants, whereby the tracer molecules, present in the plant, originate from the seed of the particular plant.
EP07834641A 2006-10-20 2007-10-18 Process for providing a plant with an identification label Withdrawn EP2078206A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07834641A EP2078206A1 (en) 2006-10-20 2007-10-18 Process for providing a plant with an identification label

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP06076900A EP1914551A1 (en) 2006-10-20 2006-10-20 Process for providing a plant with an identification label
PCT/NL2007/050503 WO2008048101A1 (en) 2006-10-20 2007-10-18 Process for providing a plant with an identification label
EP07834641A EP2078206A1 (en) 2006-10-20 2007-10-18 Process for providing a plant with an identification label

Publications (1)

Publication Number Publication Date
EP2078206A1 true EP2078206A1 (en) 2009-07-15

Family

ID=37892043

Family Applications (2)

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EP06076900A Withdrawn EP1914551A1 (en) 2006-10-20 2006-10-20 Process for providing a plant with an identification label
EP07834641A Withdrawn EP2078206A1 (en) 2006-10-20 2007-10-18 Process for providing a plant with an identification label

Family Applications Before (1)

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EP06076900A Withdrawn EP1914551A1 (en) 2006-10-20 2006-10-20 Process for providing a plant with an identification label

Country Status (2)

Country Link
EP (2) EP1914551A1 (en)
WO (1) WO2008048101A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105830758A (en) * 2016-03-30 2016-08-10 中棉种业科技股份有限公司 Method for identifying premature senescence resistance of cotton

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3543395A1 (en) * 1985-12-07 1987-06-11 J Gebhard Dr Koch Identification of plant cultivars, in particular using seeds
DE19642439C1 (en) * 1996-10-15 1997-11-20 Heinz Dr Ing Ganzelmeier Treatment of crops based on fluorescence emission
US6472219B1 (en) * 2000-08-31 2002-10-29 Ondeo Nalco Company Use of tracers to monitor application of treatment products to cut flowers
WO2004065945A1 (en) 2003-01-24 2004-08-05 Plant Research International B.V. Identification labels in plants or plant parts
DE102004017424B4 (en) * 2004-04-08 2008-10-23 Hötger, Gerhard Process for the traceable marking of agricultural bulk products for the food and feed industry
JP2006053099A (en) * 2004-08-13 2006-02-23 Institute Of Physical & Chemical Research Plant metabolic analysis method, labeled plant production method, labeled plant, and plant NMR measurement method
WO2006075953A1 (en) * 2005-01-13 2006-07-20 Swetree Technologies Ab A chemical label, plug therefore and plant with this label.

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2008048101A1 *

Also Published As

Publication number Publication date
WO2008048101A1 (en) 2008-04-24
EP1914551A1 (en) 2008-04-23

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