EP1840222A4 - The optimum method of amplification on polymerase chain reaction - Google Patents

The optimum method of amplification on polymerase chain reaction

Info

Publication number
EP1840222A4
EP1840222A4 EP05824023A EP05824023A EP1840222A4 EP 1840222 A4 EP1840222 A4 EP 1840222A4 EP 05824023 A EP05824023 A EP 05824023A EP 05824023 A EP05824023 A EP 05824023A EP 1840222 A4 EP1840222 A4 EP 1840222A4
Authority
EP
European Patent Office
Prior art keywords
materials
optimum
amplification
polymerase chain
chain reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP05824023A
Other languages
German (de)
French (fr)
Other versions
EP1840222B1 (en
EP1840222A1 (en
Inventor
Haikuo Li
Jiehuan Huang
Junhong Lv
Xiaodong Zhang
Jun Hu
Zhizhou Zhang
Chunbai Fan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SUZHOU YANGZE DELTA ACADEMY OF BIO-X SCIENCE Ltd
SUZHOU YANGZE DELTA ACADEMY OF
Original Assignee
SUZHOU YANGZE DELTA ACADEMY OF BIO-X SCIENCE Ltd
SUZHOU YANGZE DELTA ACADEMY OF
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CNB2004100991867A external-priority patent/CN1297667C/en
Priority claimed from CN 200510023780 external-priority patent/CN1289687C/en
Application filed by SUZHOU YANGZE DELTA ACADEMY OF BIO-X SCIENCE Ltd, SUZHOU YANGZE DELTA ACADEMY OF filed Critical SUZHOU YANGZE DELTA ACADEMY OF BIO-X SCIENCE Ltd
Publication of EP1840222A1 publication Critical patent/EP1840222A1/en
Publication of EP1840222A4 publication Critical patent/EP1840222A4/en
Application granted granted Critical
Publication of EP1840222B1 publication Critical patent/EP1840222B1/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

An optimum method of amplification on polymerase chain reaction, by adding metal gold materials, titanium materials, nickel materials, bismuth materials, antimony materials, selenium materials, chromium materials, or adding a mixture of above optimum materials for optimization of amplification on polymerase chain reaction, which contains the steps as follows: (1) preparation of optimum materials; (2) sterilizing the optimum materials; (3) optimization of PCR systems; (4) detection of PCR results; (5) separating the optimum materials from the reaction system.
EP05824023A 2004-12-29 2005-12-28 The optimum method of amplification on polymerase chain reaction Expired - Fee Related EP1840222B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CNB2004100991867A CN1297667C (en) 2004-12-29 2004-12-29 Method for optimizing amplification of chain reaction of nucleic acid polymerase
CN 200510023780 CN1289687C (en) 2005-02-03 2005-02-03 Optimizing method of amplifying nucleic acid polymerase chain reaction
PCT/CN2005/002345 WO2006069537A1 (en) 2004-12-29 2005-12-28 The optimum method of amplification on polymerase chain reaction

Publications (3)

Publication Number Publication Date
EP1840222A1 EP1840222A1 (en) 2007-10-03
EP1840222A4 true EP1840222A4 (en) 2009-03-04
EP1840222B1 EP1840222B1 (en) 2011-02-23

Family

ID=36614503

Family Applications (1)

Application Number Title Priority Date Filing Date
EP05824023A Expired - Fee Related EP1840222B1 (en) 2004-12-29 2005-12-28 The optimum method of amplification on polymerase chain reaction

Country Status (5)

Country Link
US (1) US7651839B2 (en)
EP (1) EP1840222B1 (en)
JP (1) JP2008525042A (en)
DE (1) DE602005026578D1 (en)
WO (1) WO2006069537A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012201475B4 (en) * 2012-02-01 2014-12-18 Gna Biosolutions Gmbh Method for multiplying nucleic acids
US9512468B2 (en) 2012-11-06 2016-12-06 Industrial Technology Research Institute Detection method uses magnetic and detectable nanoparticles with oligonucleotides attached thereto

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003048769A1 (en) * 2001-11-30 2003-06-12 Nanosphere, Inc. Real-time monitoring of pcr amplification using nanoparticle probes

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6984491B2 (en) * 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US20020090649A1 (en) * 1999-12-15 2002-07-11 Tony Chan High density column and row addressable electrode arrays
AU2001255203A1 (en) * 2000-03-28 2001-10-08 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
CN1381589A (en) * 2001-04-13 2002-11-27 缪金明 Simple and fast technique for testing mRNA chromatographic chip
GR1003966B (en) * 2001-10-16 2002-08-06 Dipping test strip assay system and assay method for the detection and/or determination of specific nucleic acid sequences
EP1361228A1 (en) * 2002-05-10 2003-11-12 Von Kiedrowski, Günter Thermostable and monoconjugatable gold cluster complexes
CN1203188C (en) * 2002-07-26 2005-05-25 中国人民解放军第三军医大学 Nano-class amplifying detection method for biochip
CN1187457C (en) * 2002-08-29 2005-02-02 上海交通大学 Prepn of bar code-type gene chip
US7300755B1 (en) * 2003-05-12 2007-11-27 Fred Hutchinson Cancer Research Center Methods for haplotyping genomic DNA

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003048769A1 (en) * 2001-11-30 2003-06-12 Nanosphere, Inc. Real-time monitoring of pcr amplification using nanoparticle probes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LI MIN ET AL: "Enhancing the efficiency of a PCR using gold nanoparticles", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 33, no. 21, 27 November 2005 (2005-11-27), pages E - 1841, XP008090941, ISSN: 0305-1048 *
See also references of WO2006069537A1 *

Also Published As

Publication number Publication date
JP2008525042A (en) 2008-07-17
DE602005026578D1 (en) 2011-04-07
US20060166240A1 (en) 2006-07-27
EP1840222B1 (en) 2011-02-23
US7651839B2 (en) 2010-01-26
EP1840222A1 (en) 2007-10-03
WO2006069537A1 (en) 2006-07-06

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