EP1800754A1 - Disposable blood centrifuge - Google Patents

Disposable blood centrifuge Download PDF

Info

Publication number
EP1800754A1
EP1800754A1 EP20050405717 EP05405717A EP1800754A1 EP 1800754 A1 EP1800754 A1 EP 1800754A1 EP 20050405717 EP20050405717 EP 20050405717 EP 05405717 A EP05405717 A EP 05405717A EP 1800754 A1 EP1800754 A1 EP 1800754A1
Authority
EP
European Patent Office
Prior art keywords
chamber
opening
blood
centrifugation
channel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP20050405717
Other languages
German (de)
French (fr)
Inventor
Jean-Denis Rochat
Original Assignee
Jean-Denis Rochat
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jean-Denis Rochat filed Critical Jean-Denis Rochat
Priority to EP20050405717 priority Critical patent/EP1800754A1/en
Publication of EP1800754A1 publication Critical patent/EP1800754A1/en
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/04Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B11/00Feeding, charging, or discharging bowls
    • B04B11/08Skimmers or scrapers for discharging ; Regulating thereof
    • B04B11/082Skimmers for discharging liquid
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B7/00Elements of centrifuges

Abstract

The device has a static admission/evacuation unit (30) comprising a pressurized gaseous fluid inlet channel emerging into a centrifugation chamber (10). An interstice is arranged in an opening (11) between the chamber and the static unit for evacuating a fluid out of the chamber. The chamber and/or the static unit slide along a revolution axis (2) between an interstice closing position and an interstice opening position.

Description

  • The present invention relates to a disposable blood centrifugation device for separating or also washing constituents of the blood. Such blood may be for example whole blood, blood already leucocyte filtered or blood bled from an operative field.
  • The most commonly used principle for separating blood components, such as red blood cells, plasma, white blood cells and platelets, from blood directly from the donor, a collection bag, an operating field or a sample blood, is the centrifugation. To do this, separators are used consisting of a centrifugation chamber rotated about a vertical axis by a motor that can rotate at high speed, generally between 1,000 and 50,000 rpm. The blood is introduced continuously or discontinuously into the centrifugation chamber through a feed channel forming part of a fixed axial member of the centrifuge device.
  • Under the effect of centrifugal force, the different blood components separate due to their unequal densities. This separation occurs naturally in a predefined order so that the blood component of higher density, namely the red blood cells, always positions itself as far as possible from the axis of revolution, while the weaker constituent density will always be closer to this axis than all other constituents. By this separation into distinct layers, these constituents can be separately extracted by collectors respectively extending into the lamination zones. By this means, each blood component is conducted separately to a outlet channel located in the fixed axial member of the device. Once extracted from the centrifuge chamber by these outlet channels, they can be collected in separate pockets or re-injected to the patient.
  • Such a device is described in more detail in the patent application EP 05405037 . When it is intended to be used in non-sterile environments, such as in blood transfusion centers or in hospitals, it is imperative to be able to guarantee the sterility of all the volumes through which the blood and its constituents will pass. In order to fulfill this requirement, the separation sets are packaged in a sterile package and are designed to define an enclosed space, sealed to any gas or fluid inlet other than the blood. For this purpose, the different collection pockets of the blood products are generally pre-connected to the separation set.
  • The sterility of this assembly would not be complete if no means were provided to ensure the possible non-contamination of the centrifuge chamber at the junction of the fixed and movable parts, namely between the axial member of admission / evacuation and the centrifuge chamber. This means must meet two main criteria which are, on the one hand to guarantee the sterility of the centrifugation device, and on the other hand to allow high rotation speeds of the centrifugation chamber by minimizing the heating between the fixed parts. and mobile device. This last criterion aims to prevent any heating of the blood by conduction within this device. Indeed, it is imperative to keep the blood at a temperature below 40 ° C to prevent its constituents from degrading.
  • In attempting to meet these criteria, various systems have been used, including the device described in the document US 3,586,413 , and known to those skilled in the art under the designation "lasso". This system consists of the arrangement of a flexible tube whose one end is integral with the center of the centrifugation chamber and the other end is secured to a fixed part of the device. The two ends of this tube give it a half-loop shape rotated around the centrifugation chamber at a speed equal to half that of the chamber. This system makes it possible to create a connection between the tube and the centrifugal chamber without friction and without any other heating than that resulting from the bending and twisting forces of the flexible tube because of its rotation on itself. The main drawbacks of this device lie in the fact that it is of complex construction, that it involves the cumbersome rotation of a tube around the centrifugation chamber and that this tube is subjected to a significant tensile stress generated by the centrifugal force to which it is subjected.
  • The document US 5,045,048 describes another device, much simpler manufacturing than the previous, wherein the junction between the fixed inlet / outlet ducts and the rotatable chamber is formed by a pair of parts having between them a very low coefficient of friction. The first piece is part of the fixed set and, in order to ensure the sealing of the connection, is in abutment against the second piece which is secured to the centrifuge chamber. This pair of parts can be achieved by means of a polymer V-ring, rotating on a metal washer, or consist of a ceramic ring based on a graphite ring. Although this system has the advantages of a low cost of implementation and simplicity in its use, it has the disadvantage of limiting the maximum speed of rotation of the chamber because of the heating induced in the friction parts.
  • To reduce this heating it has also been thought to add a plurality of ventilation vanes, disposed near the friction surface of the two parts in friction. The arrangement within a centrifuge device of such a ventilated joint contributes to improving the dissipation of heat emanating from these parts without giving full satisfaction. Such a device is described in more detail in the document EP 619'145 .
  • In the patent application EP 05'405'037 , sealing between the fixed and movable parts of the centrifuge device is achieved by means of a tubular seal. One of the ends of this seal is fixed on a cylindrical portion of the fixed axial member, while the other end is introduced into an annular space of the neck of the centrifuge chamber while bearing against a convex surface of this collar. Because of this support, the tubular seal undergoes a radial deformation which seals the centrifuge chamber. Although the diameter of the neck of the centrifuge chamber against which rubs this seal is of small diameter, the fact remains that it results in a heating directly depending on the speed of rotation of the chamber. With this system, the maximum speed of rotation is limited because of this heating. In addition, the use of chambers provided with a larger diameter neck would again remove any satisfaction in the use of this tubular joint to solve the problem of heating referred.
  • The purpose of the present invention aims to remedy at least in part the aforementioned drawbacks by suggesting a disposable blood centrifugation device which can allow, on the one hand to ensure the sterility of all the internal volumes of this device which are in contact with the blood, and secondly to eliminate any heating resulting from the rotation of this chamber around the static inlet / outlet member disposed on the axis of revolution of the chamber.
  • Another object of the present invention is to provide a centrifuge device which is also economical. Indeed, in the field of handling or blood processing, it is not uncommon that the material used is thrown after its first use. Although still functional from a functional point of view, such a centrifugation device will be intended for a single use for the reasons that are known and which aim to avoid any risk of blood contamination. It is therefore advantageous to provide a single-use device design that is as simple as possible while meeting the requirements of sterility and efficiency requirements.
  • The first advantage of this device lies in the fact that it suppresses any possible rise in temperature of the blood or its constituents. As a result, the quality of the latter is ensured since it is not altered. In addition and thanks to the object of the present invention, the speed of rotation of the centrifugation chamber is no longer dependent on the means ensuring the sterility of the device relative to a non-sterile external medium. Advantageously, the higher this speed, the shorter the residence time of the blood in the chamber in order to obtain the separation of its constituents. As a result, the treated blood flow can usefully be increased and the effectiveness of the device will also be improved.
  • Other advantages will become apparent in the light of the description which follows, referring to a preferred embodiment of the subject of the present invention, taken in a nonlimiting manner and illustrated by the appended figures in which:
    • Figure 1 is a schematic vertical sectional view of the device of the present invention shown in a so-called opening position.
    • FIG. 2 is a detailed view of a portion of the centrifugation chamber shown diagrammatically in FIG.
    • Figure 2a is a detail view of a variant of the illustration given in Figure 2.
    • Figure 3 is a view similar to that of Figure 1 illustrating the connection of a channel of the device of the present invention to an external machine.
    • Figure 4 is a schematic view, similar to that of Figure 2, showing a variant of the device of the present invention wherein the device is shown in a so-called closing position.
  • Referring to Figure 1, the disposable device of the present invention comprises a centrifuge chamber 10 having an axis of revolution 2, preferably vertical. This axis of revolution passes through an opening 11 formed in the upper part 3 of the centrifugation chamber. The latter is rotated about its axis of revolution by drive means 20, such as an electric motor whose rotation shaft 21 is secured to the lower portion 4 of the centrifuge chamber.
  • The positioning, guidance and maintenance of this rotating chamber are provided by guide means 22 preferably arranged in its upper part 3 so as to engage with the latter. These means may for example consist of three centering rollers, engaged with a tread 13 and arranged at 120 ° around the axis of revolution 2 of the chamber. Advantageously, they can be mounted retractable, for example at the end of a pivoting arm or a telescopic arm. As illustrated in the figures, the tread 13 will preferably be part of the neck 12 of the centrifugation chamber and will be formed of a perfectly circular outer surface and concentric with the axis of revolution 2. In order to take account of a eventual run-out of the centrifugation chamber, at least one centering roller could advantageously be mounted on an elastic suspension. Such an arrangement could for example be achieved by arranging a roller at the end of an arm pivotally mounted under the action of a return spring so as to ensure the permanent contact of the roller against the tread 13.
  • The opening 11 of the centrifugation chamber 10 is traversed by at least one static member 30 for admission / evacuation of the blood and at least one of its constituents. The static connotation given to this member 30 is simply to clarify that it is not rotated, unlike the centrifuge chamber. Thus, it will be understood that the device of the present invention comprises a part movable in rotation, through the opening of which is engaged at least one non-movable part in rotation. These parts may be fixed or preferably movable, relative to each other, in translation along the axis of revolution 2 as will be seen later.
  • The static inlet / outlet member 30 is engaged in the centrifuge chamber 10 through its opening 11, so that a portion of this member is located inside the centrifugation chamber 10 while another portion is outside of it. This organ comprises at least one supply channel 31 of the blood, for example from a pocket, not shown, for collecting a determined volume of blood. Alternatively, it could also be possible to treat blood directly from a donor via flexible conduits connected to the end outside the canal or, in the case of autotransfusion, to treat bleeding blood from an operative field. In accordance with the direction given by the arrow 31a, the flow of blood is continuously or intermittently from the outside to the inside of the centrifuge chamber. The static member also comprises at least one outlet channel 32 of a blood component. The flow of this component is from the inside to the outside of the chamber, according to the arrow 32a. Such a constituent will for example consist of a concentrate of red blood cells. The outlet channel 33 is a third channel which is preferably arranged within the static organ 30 and which is used to extract a second component of the blood, such as, for example, the platelet-rich plasma. The direction of flow of this second blood constituent is in accordance with the illustration given by the arrow 33a.
  • In the case of autotransfusion, an additional channel, not shown in Figure 1, may be added to introduce into the centrifugation chamber 10 a blood wash solution, such as saline, for example. This solution will be mixed with the blood in the chamber 10, then, thanks to the centrifugal forces, separated from the red blood cells by taking with it the impurities contained in the collected blood before being extracted with the plasma by the outlet channel 33.
  • The ends of the outlet channels 32, 33 are intended to be connected to flexible bags, not shown, for collecting the blood components. In the case of autotransfusion, these outputs are respectively connected to a means for re-injecting the washed component to the patient and to a bag containing washing waste. It goes without saying that the connections between these bags and the channels of the centrifuge device, are hermetic and performed under the required sterility conditions. In Generally, these connections are made in advance, following the manufacture of the centrifugation device, so that this assembly can be packaged in a preferably hermetic and sterile packaging for marketing. However, such a package may also be porous to allow subsequent sterilization with steam or gas, before use of the device.
  • According to the present invention, the static member 30 further comprises at least one inlet channel 34 of a gaseous fluid under pressure opening inside the centrifugation chamber, preferably in the upper part 3 thereof. This fluid may be pre-sterilized air from a source allowing at least to deliver this gas under a specific pressure, preferably at an adjustable flow rate. The gaseous fluid enters the inlet channel 34, in accordance with the direction of flow indicated by the arrow 34a, before emerging at the mouth 35 of this channel in the centrifugation chamber, preferably slightly below the neck 12 of the centrifuge chamber. it.
  • Although not necessary to understand the object of the present invention, it will be mentioned that below this mouth 35, the static admission / discharge member 30 comprises a plurality of collectors 37 for capturing the constituents. blood. These collectors extend radially until they reach the different zones of stratification 3a, 3b of these constituents. By these capture channels, these can be extracted simultaneously or successively from the centrifugation chamber via the outlet channels 32, 33 of the static member 30. According to the illustration given in Figures 1 and 3, the zone 3a corresponds to the one where red blood cells of higher density are found than the plasma which will be located in zone 3b, closer to the axis of revolution 2 than the previous one. This distribution occurs naturally under the effect of the centrifugal force applied to the blood spilled in the centrifuge chamber. In these figures, only the collectors 37 for capturing red blood cells have been represented. Similar collectors will also be used to capture plasma or wash waste. Because these collectors are located below the previous ones, they do not appear in the illustrations of the figures attached to this description.
  • With reference to FIG. 2, this schematically represents an enlargement of the central part of the device of the present invention. More specifically, this figure shows a detail of an embodiment of the neck 12 of the centrifuge chamber in which is engaged the static member 30 of admission / evacuation. The device is illustrated here in an open position which corresponds to the position in which it is when it is in operation.
  • As clearly visible in this figure, it is noted that a gap 38 is formed in the opening 11 of the chamber, between the latter and the static member 30 of admission / evacuation. This gap is intended to constitute an escape route for the gaseous fluid introduced under pressure into the centrifuge chamber. From the inlet channel 34, this gaseous fluid arrives in the chamber through the mouth 35. It fills the space available in this chamber by putting it under pressure. The pressure results from the pressure loss created by the flow through the gap 38. The latter will therefore be dimensioned so as to generate a measurable and sufficient overpressure in the centrifuge chamber. Thanks to this calibrated gap, the gaseous fluid escapes from this chamber in a controlled manner in a continuous flow in accordance with the direction given by the arrow 34b.
  • The main function performed by this gas flow is to repel any infiltration of germs thus protecting the centrifuge chamber and its contents from any contamination via gas escape through the gap 38. This function acts as a barrier against the infiltration of microorganisms into the device. Advantageously, no rise in temperature of the blood or its constituents will be observed. This is due to the fact that there is no longer any contact between the static member 30 and the spinning chamber 10 rotatable. Advantageously, the arrangement of this gap makes it possible to guarantee the absence of any wear between the rotating part and the non-rotating part of the device of the present invention. According to the preferred embodiment, it will be ensured that the opening 11 and the static member 30 are, around the neck 12, circular and concentric with the axis of revolution 2 so that the distribution of the gas flow through gap 38 can be as homogeneous as possible.
  • If necessary, the inlet channel 34 may be provided with at least one filter 39, for single use, sterilization of the gaseous fluid as clearly visible in FIG. 1. The arrangement of such a filter may have as its object to guarantee the sterility of the gaseous fluid so as not to contaminate the blood or its constituents. Usefully, such a filter can also serve as a sterile barrier preventing any microorganism from entering the inlet channel 34 when no other means closes the latter upstream. Advantageously, it also makes it possible to guarantee the flawless state of the fluid filtration means because it is part of the single-use disposable device of the present invention. The fluid used may be either a sterile gas or a gas pre-filtered at the source, or a gas that is intended to be purified by one or more filters 39 arranged in the upstream portion of the inlet channel 34 It should also be noted that by filter means any conventional device which allows the resulting fluid to destroy, inactivate, trap or reduce the microorganisms it contains at a level of sterility in accordance with the standards imposed in the field of transfusions and other blood manipulations.
  • According to another characteristic of the invention, provision is made for the device to be provided with a hermetic sealing means 40 of the gap 38. Such a closure means may consist of a shoulder 41 and / or a bearing 42 , of conical rounded or cylindrical shape, which can fit into the opening 11 of the centrifugation chamber or around the neck 12, more precisely fit tightly against this neck, for example in the gap 38 by translation of the one or the other of the mobile and non-mobile parts in rotation of the device. The hermetic closure of this means against the opening in the neck of the centrifugation chamber could if necessary be improved by the arrangement of an O-ring, not shown, secured to either the static member 30 or the collar 12 of the centrifuge chamber.
  • Associated with this feature, it is also provided that the centrifugation chamber 10 and / or the static admission / discharge member 30 can slide along the axis of revolution 2, between a closed position of the gap 38 and an open position thereof, and secondly hold in this open position by means of a means for automatically opening the gap 38. According to a first mode of execution, such a means of opening the gap 38 may be constituted by the pressure exerted by the gaseous fluid on the closure means 40, more particularly on the shoulder 41. When the device is in its initial configuration closed by interlocking the static member 30 in the neck 12 of the centrifugation chamber, this opening means will make it possible to automatically obtain the clearance of the gap 38 during the admission of the gaseous fluid into the centrifugation chamber . Indeed, the overpressure within the chamber will allow to expel sufficiently, or the chamber 10 downwards in the case where the static member 30 is held fixed along the axis of revolution 2, or the static member 30 upwards in the opposite case where it is the centrifugation chamber which is kept fixed along its axis of revolution.
  • According to a second possible embodiment of the opening means, the latter may be constituted by a conical portion 60 adjacent to the tread 13. This variant is given in Figure 2a which shows in detail an illustration of the collar 12 in FIG. the guide means 22 are already engaged with the tread 13. In this illustration, the presence of the conical portion 60, preferably a conical trunk portion, located just below the tread 13 is noted. The operation of this opening means according to the second embodiment is as follows.
  • When positioning the centrifugation chamber between the guide means 22, the gap 38 is initially kept closed and the guide means 22 are in the retracted position to facilitate the insertion of the chamber between them. This configuration is illustrated in Figure 4 with reference to another variant. In this initial configuration, the chamber is temporarily supported by the static member 30, which is still fitted into the centrifugation chamber and held in this position by the closure means 40. As a result, the centrifugation chamber is held in position. a slightly raised position relative to the guide means 22, as shown in FIG. 4.
  • The automatic opening of the gap 38 is obtained by a slight sliding down of the centrifugation chamber 10 along its axis of revolution 2. This sliding is obtained automatically when the guide means 22 come into contact with the room in a room first time in contact with the opening means consisting of the conical portion 60. Indeed, the support and clamping of the guide means 22 against this conical portion will push the latter down until the means of guidance are engaged with the tread 13, as shown in Figure 2a. By this slight displacement of the sliding chamber downwards, the gap 38 will be released, the lower part 4 of the chamber will be able to engage the shaft 21 of the drive means 20, and the device will be soon ready to operate.
  • Whatever the embodiment chosen, it will be noted that the opening means 40 also allows the gap 38 to be maintained in its open position, thus avoiding its inadvertent closing during the time necessary for using the device.
  • Thanks to the filter 39, the closure means 40 and the possible sliding of one or the other of the two main parts 30, 10 of the device between two relatively close positions, it is possible to condition and then sterilize, in the closed position , the device of the present invention in its packaging and to ensure the sterility of this device until its use.
  • In order to control the pressure of the gaseous fluid, it is also provided that the inlet channel 34 can be connected to a common, and therefore not shown, control or pressure regulation member within this channel. Alternatively, it will be mentioned that this member could be supplemented or replaced by a control member or regulating the flow of the gaseous fluid. Advantageously, these control means make it possible to ensure the proper functioning of the centrifugation chamber by detecting for example a possible uncontrolled leak or an abnormal throttling, or even a total obstruction or blockage of the opening 11. It will be noted that the overpressure within the centrifuge chamber can typically be of the order of 0.1 to 100 millibars for the main function of the gaseous fluid can be filled correctly.
  • With reference to the schematic illustrations given in FIGS. 1 and 3, it will be noted that the inlet channel 34 of the gaseous fluid terminates upstream by a protective membrane 45 which at least partially closes this channel. Made of a flexible and elastic material, this membrane can be in two possible configurations.
  • According to the first configuration, the protective membrane 45 hermetically closes the inlet channel 34 in order to guarantee the sterility of the entire device when it is not yet connected to the source of gaseous fluid for which it is intended. Thus, the protective membrane 45 and the closure device 40 make it possible to ensure the non-contamination of the device of the present invention as soon as it is removed from its sterile packaging, not shown, and until it is is connected to the source of gaseous fluid via the inlet channel 34. For this purpose, it is provided that this protective membrane can be pierced as is referred to in FIG.
  • In this FIG. 3, this protective membrane may, if necessary, also be applied to a lining 46 integral with the upstream end portion of the inlet channel 34. Indeed, it is provided that the device of the present invention can be connected to a machine 50 for treating blood fluids. The wall of the machine will then comprise, for example, a tubular tip 51 for perforating the protective membrane 45. After perforation of this membrane, the insertion of this circular tip through the membrane will cause a natural deformation thereof until 'to present an elastic lip 47. This lip will surround the tubular tip and come to be plated by acting as joint sealing. Usefully, such a seal will prevent the gaseous fluid from escaping out of the liner 46. This same machine is equipped with the means necessary to produce or connect to a source of gaseous fluid under pressure, preferably sterile or pre-sterilized.
  • According to the second possible configuration of this membrane, it is expected that it is provided with a circular opening of diameter smaller than that of the tubular nozzle 51, and that it therefore only partially closes the inlet channel 34 In this case, the seal 46 will be sealed only after insertion of the tubular nozzle through the circular opening formed in advance within this membrane, thanks to the elastic lip 47 that constitutes the part peripheral of the circular opening. In the same way, this lip will be automatically pressed against the tubular end because of the diameter of the latter which is greater than that of the circular opening previously formed in the membrane.
  • The purpose of the arrangement of this membrane is then above all to ensure the seal between the tubular nozzle 51 and the device during its operation. It makes it possible to prevent the gaseous fluid injected into the channel 34a from escaping from the device before being passed through the centrifugation chamber 10.
  • When connecting the disposable device to the machine 50, the tubular nozzle 51 on the one hand allows the piercing of the membrane 45 or the widening of its existing circular opening, and on the other hand acts as a delivery channel of the gaseous fluid within the intake channel 34.
  • Referring to Figure 4, it shows schematically an enlargement of the central portion of a variant of the device of the present invention. More precisely, this figure shows a detail of a second mode embodiment of the neck 12 of the centrifuge chamber, in which is engaged the static member 30 of admission / evacuation. The latter is illustrated in an initial position, called the closed position, which corresponds for example to the position in which the device is located when it leaves its packaging packaging. In this figure, it will be noted that the leakage path of the gaseous fluid out of the centrifugation chamber is via a gap 38 with sinuous shapes. The labyrinth shape given to this gap is defined by the arrangement of baffles 36 which occupy at least part of the space of this interstice. These baffles may be integral with either the static member 30 or the neck 12 of the centrifugation chamber, or still partly of the static member 30 and partly of this chamber as shown in Figure 4.
  • Advantageously, the arrangement of these baffles makes it possible, for the same gas flow rate, to increase the pressure drop within the exhaust path constituted by the gap 38. Thus, the overpressure in the centrifugation chamber will be all the more important. Advantageously, the arrangement of such baffles improves the sealing of the disposable device, particularly where the static member 30 passes through the neck 12 of the centrifuge chamber. The shape that the gap 38 takes can be more or less complex depending on the number, the position and the shape of the baffles 36.
  • According to a preferred embodiment, the parts of this device are intended to be made by injection of a plastic material into a mold. The dimensions of the centrifugation chamber are of the order of 20 to 200 mm in length for a diameter of between 10 and 100 mm approximately. In addition, the parts that constitute this device are mainly cylindrical in shape which facilitates their injection. The realization of such a device by the An injection method is therefore perfectly suited to this type of implementation because of its small size, its single-use nature and the imperatives to obtain limited manufacturing costs.
  • Finally, it will also be mentioned that the subject of the present invention could also be used for blood analysis purposes from samples or else to perform other blood manipulations such as the washing of red blood cells, for the purpose of autotransfusion in particular, or deglycerolization of frozen red blood cells.

Claims (13)

  1. Disposable blood centrifugation device for separating or also washing blood components comprising a centrifugation chamber (10) having an axis of revolution (2) and an opening (11) therethrough and formed in an upper part (3) ) of said centrifugation chamber (10), means (22) for rotating said centrifugation chamber (10) for engaging said centrifugal chamber against a tread (13), driving means ( 20) in rotation of this centrifugation chamber around its axis of revolution (2), at least one static inlet / outlet member (30) engaged in said centrifugation chamber (10) by its opening (11) and comprising at least one at least one delivery channel (31) of said blood and at least one outlet channel (32, 33) of a constituent of said blood, characterized in that said static admission / evacuation member (30) comprises at least one intake channel (34) of a fluid pressurized gas emerging inside the centrifuge chamber (10) and in that a gap (38) is formed in said opening (11) between the centrifugation chamber (10) and the static member (30) intake / exhaust system for exhausting said gaseous fluid from said centrifuge chamber (10).
  2. Device according to claim 1, characterized in that the centrifugation chamber (10) or / and the static admission / evacuation member (30) slides along the axis of revolution (2) between a closed position of the gap (38) and an open position thereof.
  3. Device according to claim 1 or 2, characterized in that it comprises a hermetic closure means (40) of the gap (38).
  4. Device according to claim 3, characterized in that said closing means (40) is constituted by a shoulder (41) and / or by a bearing surface (42) which can fit into the opening (11) of said centrifuge chamber (10).
  5. Device according to claim 2, characterized in that it comprises means for automatically opening the gap (38).
  6. Device according to claim 5, characterized in that said opening means is constituted by the pressure exerted by said gaseous fluid on said closure means (40).
  7. Device according to claim 5, characterized in that said opening means is constituted by a conical portion (60) adjacent to said tread (13).
  8. Device according to claim 1, characterized in that baffles (36) occupy at least part of the space of the gap (38).
  9. Device according to claim 1, characterized in that the inlet channel (34) of the gaseous fluid is provided with at least one disposable filter (39) for sterilizing the gaseous fluid.
  10. Device according to claim 1, characterized in that a protective membrane (45) at least partially closes the inlet channel (34) upstream thereof.
  11. Device according to claim 10, characterized in that said protective membrane (45) allows the insertion within it of a tubular nozzle (51).
  12. Device according to claim 11, characterized in that said protective membrane (45) forms a lip resilient (47) surrounding the tubular tip (51) when the protective membrane is traversed by the latter.
  13. Device according to claim 1, characterized in that the admission channel (34) of the gaseous fluid is connected to a control or pressure regulating member within this channel and / or to a control or regulation of the flow of the gaseous fluid.
EP20050405717 2005-12-21 2005-12-21 Disposable blood centrifuge Withdrawn EP1800754A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP20050405717 EP1800754A1 (en) 2005-12-21 2005-12-21 Disposable blood centrifuge

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP20050405717 EP1800754A1 (en) 2005-12-21 2005-12-21 Disposable blood centrifuge
EP20060817753 EP1965925B1 (en) 2005-12-21 2006-12-14 Disposable device for centrifugation of blood
US12/158,119 US8070666B2 (en) 2005-12-21 2006-12-14 Disposable device for centrifugation of blood
AT06817753T AT544524T (en) 2005-12-21 2006-12-14 Disposable device for centrifuging blood
PCT/CH2006/000701 WO2007071086A1 (en) 2005-12-21 2006-12-14 Disposable device for centrifugation of blood

Publications (1)

Publication Number Publication Date
EP1800754A1 true EP1800754A1 (en) 2007-06-27

Family

ID=36274050

Family Applications (2)

Application Number Title Priority Date Filing Date
EP20050405717 Withdrawn EP1800754A1 (en) 2005-12-21 2005-12-21 Disposable blood centrifuge
EP20060817753 Active EP1965925B1 (en) 2005-12-21 2006-12-14 Disposable device for centrifugation of blood

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP20060817753 Active EP1965925B1 (en) 2005-12-21 2006-12-14 Disposable device for centrifugation of blood

Country Status (4)

Country Link
US (1) US8070666B2 (en)
EP (2) EP1800754A1 (en)
AT (1) AT544524T (en)
WO (1) WO2007071086A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107635668A (en) * 2015-05-07 2018-01-26 生物安全股份有限公司 For by biofluid continuous processing and being separated into the devices, systems, and methods of component

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1683579A1 (en) * 2005-01-25 2006-07-26 Jean-Denis Rochat Disposable device for the continuous separation by centrifugation of a physiological liquid
EP1800754A1 (en) * 2005-12-21 2007-06-27 Jean-Denis Rochat Disposable blood centrifuge
EP1911520A1 (en) * 2006-10-10 2008-04-16 Jean-Denis Rochat Disposable set for separating blood or for washing blood components
WO2012137086A1 (en) 2011-04-08 2012-10-11 Sorin Group Italia S.R.L. Disposable device for centrifugal blood separation
US10039876B2 (en) 2014-04-30 2018-08-07 Sorin Group Italia S.R.L. System for removing undesirable elements from blood using a first wash step and a second wash step
US10040077B1 (en) * 2015-05-19 2018-08-07 Pneumatic Scale Corporation Centrifuge system including a control circuit that controls positive back pressure within the centrifuge core
US10683478B1 (en) * 2019-05-16 2020-06-16 Shenzhen Eureka biotechnology Co. Ltd Device and system for processing a liquid sample containing cells

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3073517A (en) * 1959-04-07 1963-01-15 Beckman Instruments Inc Continuous flow centrifuge apparatus and rotor therefor
US3616992A (en) * 1969-06-06 1971-11-02 James S Deacon Partial vacuum centrifugal separator
US4417885A (en) * 1981-10-22 1983-11-29 Westfalia Separator Ag Centrifuge with vertical axis of rotation
US5045048A (en) * 1990-03-29 1991-09-03 Haemonetics Corporation Rotary centrifuge bowl and seal for blood processing
US5851169A (en) * 1996-01-31 1998-12-22 Medtronic Electromedics, Inc. Rotary plate and bowl clamp for blood centrifuge

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3601307A (en) * 1970-03-19 1971-08-24 Pennwalt Corp Centrifuge with spindle-sealing means
EP0568191B1 (en) * 1992-04-29 1997-05-28 Cobe Laboratories, Inc. Centrifuge having a single swing arm for retaining a stator tube
JPH0775746A (en) 1993-04-05 1995-03-20 Electromedics Inc Rotating sealing member for centrifugal separator
US5505683A (en) * 1993-12-10 1996-04-09 Haemonetics Corporation Centrifuge bowl gripping apparatus having a retaining arm with a stationary jaw and a moveable jaw
US5514070A (en) * 1994-01-21 1996-05-07 Haemonetics Corporation Plural collector centrifuge bowl for blood processing
DE19500600C1 (en) * 1995-01-11 1996-02-08 Westfalia Separator Ag Solid sleeve centrifuge for separating fluid or solids mixture
JPH09192215A (en) * 1996-01-17 1997-07-29 Takaharu Nakane Centrifugal bowl
US7717274B2 (en) * 2004-06-09 2010-05-18 The Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Device and method for preparing washed red blood cells for newborn transfusions
EP1683578A1 (en) * 2005-01-25 2006-07-26 Jean-Denis Rochat Centrifugal separator for a physiological liquid, in particular blood
EP1800754A1 (en) * 2005-12-21 2007-06-27 Jean-Denis Rochat Disposable blood centrifuge

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3073517A (en) * 1959-04-07 1963-01-15 Beckman Instruments Inc Continuous flow centrifuge apparatus and rotor therefor
US3616992A (en) * 1969-06-06 1971-11-02 James S Deacon Partial vacuum centrifugal separator
US4417885A (en) * 1981-10-22 1983-11-29 Westfalia Separator Ag Centrifuge with vertical axis of rotation
US5045048A (en) * 1990-03-29 1991-09-03 Haemonetics Corporation Rotary centrifuge bowl and seal for blood processing
US5851169A (en) * 1996-01-31 1998-12-22 Medtronic Electromedics, Inc. Rotary plate and bowl clamp for blood centrifuge

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107635668A (en) * 2015-05-07 2018-01-26 生物安全股份有限公司 For by biofluid continuous processing and being separated into the devices, systems, and methods of component
CN107635668B (en) * 2015-05-07 2019-12-03 生物安全股份有限公司 For by biofluid continuous processing and being separated into the devices, systems, and methods of component
US10562041B2 (en) 2015-05-07 2020-02-18 Biosafe S.A. Device, system and method for the continuous processing and separation of biological fluids into components
US10773262B2 (en) 2015-05-07 2020-09-15 Biosafe S.A. Device, system and method for the continuous processing and separation of biological fluids into components

Also Published As

Publication number Publication date
WO2007071086A1 (en) 2007-06-28
AT544524T (en) 2012-02-15
US8070666B2 (en) 2011-12-06
EP1965925A1 (en) 2008-09-10
US20080264841A1 (en) 2008-10-30
EP1965925B1 (en) 2012-02-08

Similar Documents

Publication Publication Date Title
JP5944935B2 (en) Method for controlling the operation of a blood treatment system
US6102883A (en) Blood collection and separation process
CA2480236C (en) Improved apparatus for the continuous separation of biological fluids into components and method of using same
US5494578A (en) Centrifugation pheresis system
EP0839072B1 (en) Blood processing system
CA2452055C (en) Integrated automatic blood collection and processing unit
EP1757318B1 (en) Apparatus and method for separating a composite liquid into at least two components
EP0608519B1 (en) Centrifugal separator
US10258998B2 (en) Optical monitoring system for blood processing system
US4921473A (en) Multicomponent fluid separation and irradiation system
US5076911A (en) Centrifugation chamber having an interface detection surface
US4204537A (en) Process for pheresis procedure and disposable plasma
US7168571B2 (en) Inverting-filter centrifuge
EP0299054B1 (en) Centrifugation pheresis system
US7476209B2 (en) Method and apparatus for collecting a blood component and performing a photopheresis treatment
US9388377B2 (en) Regenerative cell extraction unit and regenerative cell extraction system
RU2577447C2 (en) Surgical approach devices with sorbent agents
AU670880B2 (en) Separation of cells from a cellular suspension
JP6398104B2 (en) Centrifuge device, centrifuge, and inspection kit including centrifuge
US7582049B2 (en) Fluid separation devices, systems and/or methods using a centrifuge and roller pump
DE4132716C2 (en) Process for washing cells
US7396451B2 (en) Methods and apparatus for blood component separation
US6733433B1 (en) Blood separation system particularly for concentrating hematopoietic stem cells
US3672564A (en) Rotary fluid seal and distribution means for centrifuges
JP5740692B2 (en) Serum preparation device

Legal Events

Date Code Title Description
AX Extension of the european patent to

Countries concerned: ALBAHRMKYU

AK Designated contracting states:

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

AKX Payment of designation fees
REG Reference to a national code

Ref country code: DE

Ref legal event code: 8566

18D Deemed to be withdrawn

Effective date: 20071228