EP1272520A2 - Gpcr-proteins and nucleic acids encoding same - Google Patents

Gpcr-proteins and nucleic acids encoding same

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Publication number
EP1272520A2
EP1272520A2 EP01930484A EP01930484A EP1272520A2 EP 1272520 A2 EP1272520 A2 EP 1272520A2 EP 01930484 A EP01930484 A EP 01930484A EP 01930484 A EP01930484 A EP 01930484A EP 1272520 A2 EP1272520 A2 EP 1272520A2
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
polypeptide
amino acid
novx
iii
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01930484A
Other languages
German (de)
French (fr)
Inventor
Suresh Shenoy
Esha A. Gangolli Gangolli
Luca Rastelli
Glennda Smithson
Muralidhara Padigaru
Corine A. M. Vernet
Adam R. Wolenc
Stacie J. Casman
Velizar T. Tchernev
Edward S. Szekeres
William Gorsse
John P. Alsobrook
Catherine E. Burgess
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CuraGen Corp
Original Assignee
CuraGen Corp
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Filing date
Publication date
Priority claimed from US09/826,734 external-priority patent/US20030017457A1/en
Application filed by CuraGen Corp filed Critical CuraGen Corp
Publication of EP1272520A2 publication Critical patent/EP1272520A2/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Definitions

  • the present invention generally relates to novel proteins encoded by genomic DNA sequences and proteins similar to them, namely, new proteins bearing sequence similarity to G-protein coupled receptors (GPCRs), nucleic acids that encode these proteins or fragments thereof, and antibodies that bind immunospecifically to a protein of the invention.
  • GPCRs G-protein coupled receptors
  • Olfactory receptors have been identified as an extremely large subfamily of GPCRs in a number of species. These receptors share a seven transmembrane domain structure with many neurotransmitter and hormone receptors, and are likely to underlie the recognition of G-protein-mediated transduction of odorant signals.
  • ORs Olfactory receptors
  • the invention is based, in part, upon the discovery of novel polynucleotide sequences encoding novel polypeptides.
  • the invention provides an isolated nucleic acid molecule that includes the sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 or a fragment, homolog, analog or derivative thereof.
  • the nucleic acid can include, e.g., a nucleic acid sequence encoding a polypeptide at least 85% identical to a polypeptide that includes the amino acid sequences of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24.
  • the nucleic acid can be, e.g., a genomic DNA fragment, or a cDNA molecule.
  • Also included in the invention is a vector containing one or more of the nucleic acids described herein, and a cell containing the vectors or nucleic acids described herein.
  • the invention is also directed to host cells transformed with a vector comprising any of the nucleic acid molecules described above.
  • the invention includes a pharmaceutical composition that includes a NOVX nucleic acid and a pharmaceutically acceptable carrier or diluent.
  • the invention includes a substantially purified NOVX polypeptide, e.g., any of the NOVX polypeptides encoded by a NOVX nucleic acid, and fragments, homologs, analogs, and derivatives thereof.
  • the invention also includes a pharmaceutical composition that includes a NOVX polypeptide and a pharmaceutically acceptable carrier or diluent.
  • the invention provides an antibody that binds specifically to a NOVX polypeptide.
  • the antibody can be, e.g., a monoclonal or polyclonal antibody, and fragments, homologs, analogs, and derivatives thereof.
  • the invention also includes a pharmaceutical composition including NOVX antibody and a pharmaceutically acceptable carrier or diluent.
  • the invention is also directed to isolated antibodies that bind to an epitope on a polypeptide encoded by any of the nucleic acid molecules described above.
  • the invention also includes kits comprising any of the pharmaceutical compositions described above.
  • the invention further provides a method for producing a NOVX polypeptide by providing a cell containing a NOVX nucleic acid, e.g., a vector that includes a NOVX nucleic acid, and culturing the cell under conditions sufficient to express the NOVX polypeptide encoded by the nucleic acid.
  • the expressed NOVX polypeptide is then recovered from the cell.
  • the cell produces little or no endogenous NOVX polypeptide.
  • the cell can be, e.g., aprokaryotic cell or eukaryotic cell.
  • the invention is also directed to methods of identifying a NOVX polypeptide or nucleic acid in a sample by contacting the sample with a compound that specifically binds to the polypeptide or nucleic acid, and detecting complex formation, if present.
  • the invention further provides methods of identifying a compound that modulates the activity of a NOVX polypeptide by contacting a NOVX polypeptide with a compound and determining whether the NOVX polypeptide activity is modified.
  • the invention is also directed to compounds that modulate NOVX polypeptide activity identified by contacting a NOVX polypeptide with the compound and determining whether the compound modifies activity of the NOVX polypeptide, binds to the NOVX polypeptide, or binds to a nucleic acid molecule encoding a NOVX polypeptide.
  • the invention provides a method of determining the presence of or predisposition of a NOVX-associated disorder in a subject. The method includes providing a sample from the subject and measuring the amount of NOVX polypeptide in the subject sample. The amount of NOVX polypeptide in the subject sample is then compared to the amount of NOVX polypeptide in a control sample.
  • An alteration in the amount of NOVX polypeptide in the subject protein sample relative to the amount of NOVX polypeptide in the control protein sample indicates the subject has a tissue proliferation-associated condition.
  • a control sample is preferably taken from a matched individual, i.e., an individual of similar age, sex, or other general condition, but who is not suspected of having a tissue proliferation- associated condition.
  • the control sample may be taken from the subject at a time when the subject is not suspected of having a tissue proliferation-associated disorder.
  • the NOVX is detected using a NOVX antibody.
  • the method includes providing a nucleic acid sample, e.g., RNA or DNA, or both, from the subject and measuring the amount of the NOVX nucleic acid in the subject nucleic acid sample.
  • the amount of NOVX nucleic acid sample in the subject nucleic acid is then compared to the amount of a NOVX nucleic acid in a control sample.
  • An alteration in the amount of NOVX nucleic acid in the sample relative to the amount of NOVX in the control sample indicates the subject has a NOVX- associated disorder.
  • the invention provides a method of treating or preventing or delaying a NOVX-associated disorder.
  • the method includes administering to a subject in which such treatment or prevention or delay is desired a NOVX nucleic acid, a NOVX polypeptide, or a NOVX antibody in an amount sufficient to treat, prevent, or delay a NOVX- associated disorder in the subject.
  • NOV1-12 are homologous to members of the odorant receptor (OR) family of the human G-protein coupled receptor (GPCR) superfamily of proteins, as shown in Table 56.
  • OR odorant receptor
  • GPCR human G-protein coupled receptor
  • Olfactory receptors are the largest family of G-protein-coupled receptors
  • GPCRs GPCRs and belong to the first family (Class A) of GPCRs, along with catecholamine receptors and opsins.
  • the OR family contains over 1,000 members that traverse the phylogenetic spectrum from C. elegans to mammals. ORs most likely emerged from prototypic GPCRs several times independently, extending the structural diversity necessary both within and between species in order to differentiate the multitude of ligands. Individual olfactory sensory neurons are predicted to express a single, or at most a few, ORs. All ORs are believed to contain seven ⁇ -helices separated by three extracellular and three cytoplasmic loops, with an extracellular amino-terminus and a cytoplasmic carboxy-terminus.
  • the pocket of OR ligand binding is expected to be between the second and sixth transmembrane domains of the proteins.
  • Overall amino acid sequence identity within the mammalian OR family ranges from 45% to >80%, and genes greater than 80% identical to one another at the amino acid level are considered to belong to the same subfamily. Since the first ORs were cloned in 1991, outstanding progress has been made into their mechanisms of action and potential dysregulation during disease and disorder. It is understood that some human diseases result from rare mutations within GPCRs. Drug discovery avenues could be used to produce highly specific compounds on the basis of minute structural differences of OR subtypes, which are now being appreciated with in vivo manipulation of OR levels in transgenic and knock-out animals.
  • OR proteins bind odorant ligands and transmit a G-protein-mediated intracellular signal, resulting in generation of an action potential.
  • the accumulation of DNA sequences of hundreds of OR genes provides an opportunity to predict features related to their structure, function and evolutionary diversification. See Pilpel Y, et.al., Essays Biochem 1998;33:93- 104.
  • the OR repertoire has evolved a variable ligand-binding site that ascertains recognition of multiple odorants, coupled to constant regions that mediate the cAMP-mediated signal transduction.
  • the cellular second messenger underlies the responses to diverse odorants through the direct gating of olfactory-specific cation channels. This situation necessitates a mechanism of cellular exclusion, whereby each sensory neuron expresses only one receptor type, which in turn influences axonal projections.
  • a 'synaptic image' of the OR repertoire thus encodes the detected odorant in the central nervous system.
  • ORs odorant receptors
  • ORs are expressed by nasal olfactory sensory neurons, and each neuron expresses only 1 allele of a single OR gene.
  • different sets of ORs are expressed in distinct spatial zones. Neurons that express the same OR gene are located in the same zone; however, in that zone they are randomly interspersed with neurons expressing other ORs.
  • the cell chooses an OR gene for expression, it may be restricted to a specific zonal gene set, but it may select from that set by a stochastic mechanism.
  • Proposed models of OR gene choice fall into 2 classes: locus-dependent and locus-independent.
  • Locus-dependent models posit that OR genes are clustered in the genome, perhaps with members of different zonal gene sets clustered at distinct loci. In contrast, locus-independent models do not require that OR genes be clustered.
  • OR genes have been mapped to 11 different regions on 7 chromosomes. These loci lie within paralogous chromosomal regions that appear to have arisen by duplications of large chromosomal domains followed by extensive gene duplication and divergence. Studies have shown that OR genes expressed in the same zone map to numerous loci; moreover, a single locus can contain genes expressed in different zones.
  • Issel-Tarver and Rine characterized 4 members of the canine olfactory receptor gene family.
  • the 4 subfamilies comprised genes expressed exclusively in olfactory epithelium. Analysis of large DNA fragments using Southern blots of pulsed field gels indicated that subfamily members were clustered together, and that two of the subfamilies were closely linked in the dog genome. Analysis of the four olfactory receptor gene subfamilies in 26 breeds of dog provided evidence that the number of genes per subfamily was stable in spite of differential selection on the basis of olfactory acuity in scent hounds, sight hounds, and toy breeds.
  • Issel-Tarver and Rine performed a comparative study of four subfamilies of olfactory receptor genes first identified in the dog to assess changes in the gene family during mammalian evolution, and to begin linking the dog genetic map to that of humans.
  • These four families were designated by them OLF1, OLF2, OLF3, and OLF4 in the canine genome.
  • the subfamilies represented by these four genes range in size from 2 to 20 genes. They are all expressed in canine olfactory epithelium but were not detectably expressed in canine lung, liver, ovary, spleen, testis, or tongue.
  • the OLF1 and OLF2 subfamilies are tightly linked in the dog genome and also in the human genome.
  • the smallest family is represented by the canine OLF1 gene.
  • dog gene probes individually to hybridize to Southern blots of genomic DNA from 24 somatic cell hybrid lines. They showed that the human homologous OLF1 subfamily maps to human chromosome 11. The human gene with the strongest similarity to the canine OLF2 gene also mapped to chromosome 11. Both members of the human subfamily that hybridized to canine OLF3 were located on chromosome 7. It was difficult to determine to which chromosome or chromosomes the human genes that hybridized to the canine OLF4 probe mapped. This subfamily is large in mouse and hamster as well as human, so the rodent background largely obscured the human cross-hybridizing bands.
  • Issel-Tarver and Rine demonstrated that in the human OLF1 and OLF2 homologs are likewise closely linked. By studying YACs, Issel-Tarver and Rine (1997) found that the human OLF3 homolog maps to 7q35.
  • a chromosome 19-specific cosmid library was screened by hybridization with the canine OLF4 gene probe, and clones that hybridized strongly to the probe even at high stringency were localized to 19pl3.1 and 19pl3.2. These clones accounted, however, for a small fraction of the homologous human bands. Rouquier et al. (1998) demonstrated that members of the olfactory receptor gene family are distributed on all but a few human chromosomes.
  • OR sequences reside at more than 25 locations in the human genome. Their distribution was biased for terminal bands of chromosome arms. Flow-sorted chromosomes were used to isolate 87 OR sequences derived from 16 chromosomes. Their sequence relationships indicated the inter- and intrachromosomal duplications responsible for OR family expansion. Rouquier et al. (1998) determined that the human genome has accumulated a striking number of dysfunctional copies: 72% of these sequences were found to be pseudogenes. ORF-containing sequences predominate on chromosomes 7, 16, and 17.
  • telomere a subtelomeric DNA duplication that provided insight into the variability, complexity, and evolutionary history of that unusual region of the human genome, the telomere.
  • OR olfactory receptor
  • ORs odorant receptor
  • Buck and Axel (1991) discovered this large family of genes encoding putative odorant receptor genes. Zhao et al. (1998) provided functional proof that one OR gene encodes a receptor for odorants.
  • the isolation of OR genes from the rat by Buck and Axel (1991) was based on three assumptions. First, ORs are likely G protein-coupled receptors, which characteristically are 7-transmembrane proteins. Second, ORs are likely members of a multigene family of considerable size, because an immense number of chemicals with vastly different structures can be detected and discriminated by the vertebrate olfactory system. Third, ORs are likely expressed selectively in olfactory sensory neurons. Ben-Arie et al.
  • the human OR genes differ markedly from their counterparts in other species by their high frequency of pseudogenes, except the testicular OR genes. Research showed that individual olfactory sensory neurons express a small subset of the OR repertoire. In rat and mouse, axons of neurons expressing the same OR converge onto defined glomeruli in the olfactory bulb.
  • the present invention generally relates to novel proteins encoded by genomic DNA sequences and proteins similar to them, namely, new proteins bearing sequence similarity to GPCRs, nucleic acids that encode these proteins or fragments thereof, and antibodies that bind immunospecifically to a protein of the invention. Included in the invention are the novel nucleic acid sequences and their polypeptides.
  • NOVX nucleic acids or “NOVX polynucleotides” and the corresponding encoded polypeptides are referred to as “NOVX polypeptides” or “NOVX proteins.” Unless indicated otherwise, “NOVX” is meant to refer to any of the novel sequences disclosed herein.
  • Table 1 provides a summary of the NOVX nucleic acids and their encoded polypeptides. Example 1 provides a description of how the novel nucleic acids were identified.
  • NOVX nucleic acids and their encoded polypeptides are useful in a variety of applications and contexts.
  • the various NOVX nucleic acids and polypeptides according to the invention are useful as novel members of the protein families according to the presence of domains and sequence relatedness to previously described proteins. Additionally, NOVX nucleic acids and polypeptides can also be used to identify proteins that are members of the family to which the NOVX polypeptides belong.
  • NOV1 through NOV12 are homologous to members of the GPCR family of proteins that are important in maintaining G-protein mediated signal transduction.
  • the NOV1 nucleic acids, polypeptides, antibodies and related compounds according to the invention will be useful in therapeutic and diagnostic applications in disorders characterized by G-protein signaling, e.g., bacterial, fungal, protozoal and viral infections, pain, cancer, anorexia, bulimia, asthma, and Parkinson's disease.
  • the NOVX nucleic acids and polypeptides can also be used as immunogens to produce antibodies specific to the invention, and to screen for molecules, which inhibit or enhance NOVX activity or function.
  • a NOVl sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins.
  • a NOVl nucleic acid is found on human chromosome 11.
  • a NOVl nucleic acid and its encoded polypeptide includes the sequences shown in Table 2A.
  • the disclosed nucleic acid (SEQ ID NO.: 1) is 978 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 21-23 and ends with a TAA stop codon at nucleotides 954-956.
  • a putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 2A, and the start and stop codons are in bold letters.
  • the representative ORF encodes a 311 amino acid polypeptide (SEQ ID NO. : 2) with a predicted molecular weight of 34796.5 daltons (Da).
  • PSORT analysis of a NOVl polypeptide predicts a plasma membrane protein with a certainty of 0.6400 and an endoplasmic reticulum membrane protein with a certainty of 0.6850.
  • SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 55 and 56 in SEQ ID NO.: 2.
  • a NOVl nucleic acid has homology (67% identity) to a Mus musculus GPCR mRNA 15 (MUSI; GENBANK-ID: AF121979), as is shown in Table 2B.
  • a NOVl polypeptide also has homology (63% identity, 80% similarity) with the 318 amino acid residue protein from Mus musculus (MUS2; SPTREMBL-ACC: Q9WU93), as is shown in Table 2C.
  • NOVl expression was analyzed by TaqMan analysis. Results shown in Example 4, Table 14C demonstate that NOVl expression is higher in lung cancer (small cell), colon cancer, ovarian cancer, prostate cancer and uterine cancer as compared to normal tissue.
  • a NOV2 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins.
  • a NOV2 nucleic acid is found on human chromosome 11.
  • a NOV2 nucleic acid and its encoded polypeptide include the sequences shown in Table 3 A.
  • the disclosed nucleic acid (SEQ ID NO.: 3) is 983 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 21-23 and ends with a TAA stop codon at nucleotides 960-962.
  • a putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 3A, and the start and stop codons are in bold letters.
  • the representative ORF encodes a 313 amino acid polypeptide (SEQ ID NO.: 4) with a predicted molecular weight of 35077.8 Da.
  • PSORT analysis of a NOV2 polypeptide predicts a plasma membrane protein with a certainty of 0.600. Using SIGNALP analysis, it is predicted that a NOV2 polypeptide has no signal peptide.
  • a NOV2 polypeptide has homology (51% identity, 70% similarity) with a 319 amino acid residue olfactory receptor-like protein from Gallus gallus (GALl ; SPTREMBL-ACC: Q9YH55), as shown in Table 3C.
  • a NOV2 polypeptide also has homology (46% identity, 67% similarity) to a HOR 5'BETA3 polypeptide from Homo sapiens (HORl; SPTREMBL-ACC: Q9Y5P1), as is shown in Table 3D.
  • NOV2 201 TCACCACCTTCTTCTTGGTTGGGATGCCAGGGCTAGAATATGCACACATCTGGATCTCTA 260
  • NOV2 261 TCCCCATCTGCAGCATGTATCTTATTGCTATTCTAGGAAATGGCACCATTCTTTTTATCA 320
  • NOV2 321 TCAAGACAG-AGC-CCTCCTTGCATGGGCCCATGTACTATTTTCTTTCCATGTTGGCTAT 378
  • GALl 29158 TGA-GAGTGCATCGCCAGCTC-CACCAGCCCATGTACTATTTCCTTTTGATGCTGGCCAC 29215
  • GALl ' 29216 CACAGACCTGGGCCTGACTCTGTCCACCCTGCCCACCGTTCTGCGCGTCTTCTGGTTGGG 29275
  • GALl 29276 TGCCATGGAAATCAGCTTCCCC-GCCTGCCTCATCCAGATGTTCTGCATCCATGTCTTTT 29334
  • NOV2 498 CAGTACTGGAGTCCTCAGTCCTCCTGATCATGTCATTTGATAGATTCCTAGCCATCCACA 557
  • GALl 29335 CCTTCATGGAGTCCTCAGTGCTCCTGGCCATGGCCTTTGATCGCTATGTGGCCATCTGCT 29394
  • NOV2 558 ATCCTCTGAGATACACCTCAATCCTGACAACTGTCAGAGTTGCCCAAATAGGGATAGTAT 617
  • GALl 29395 GCCCGCTGAGGTACTCCTCCATCCTCACTGGTGCCAGGGTTGCACAGATTGGACTGGGGA 29454
  • GALl 29455 TCATCTGCC-GATGCACACTGTCACTTCTCCCATTAATCTGCCTCCTGACGTGGCT-GCC 29512
  • NOV2 676 ATATTGCAAGAAAAACCAATTATCCCATTCCTACTGTCTCCACCAGGATGTCATGAAGT- 734
  • GALl 29631 -ATCCTGGACTTTGTTCTCATTGCGTTGTCCTACATCATGATCTTTAGGACTGTGCTAGG 29689
  • N0V2 853 AATTGCATCCAAAAAGGAGGAGCTTAAGGCTCTCAATACTTGTGTTTCACACATCTGTGC 912
  • GALl 29690 CATCACCTCCAAAGAGGAGCAGACCAAAGCCCTAAACACTTGTGTCTCTCACTTCTGTGC 29749
  • NOV2 913 AGTGATCATCTTCTACCTGCCCATCATCAAC-CTGGCCGTTGTCCACCGCTTTGCCGGGC 971 II IN I
  • GALl 29750 CGTCCTCATCTTCTACAT-CCCTTTGGCTGGGCTGTCCATTATACACCGGTATGGAAGGA 29808
  • GALl 29809 ATGCCCCACCTATTAGCCATGCTGTCATGGCCAATGT-CTACCTCTTTGTCCCACCTATA 29867 NOV2: 1031 ATGAAACCAATTGTTTATTGTGTAAAAACTAA 1062 (SEQ ID NO.: 31)
  • OLR1 252 IPLAGLSIIHRYGRNAPPISHAVMANVYLFVPPILNPVLYSMKSKAICKGLLRLLCQ 308 NOV2 (SEQ ID NO. : 33) OLR1 (SEQ ID NO. : 34)
  • NOV2 74 GLSLSSLPTVLSIFLFNAPETSSSACFAQEFFIHGFSVLESSVLLIMSFDRFLAIHNPLR 133
  • HORl 70 MVTLTTMPTVMGIL VNHREISSVGCFLQAYFIHSLSVVESGSLLAMAYDRFIAIRNPLR 129 NOV2: 134 YTSILTTVRVAQIGIVFSFKSMLLVLPFPFTLRSLRYCKKNQLSHSYCLHQDVMKLACSD 193 I I I I I + 1 + + + + 1 1 I I I I + ++ ++ M M ++ I + M l + l
  • HORl 190 ITFNRLYPVILISLTIFLDSLIILFSYILILNTVIGIASGEERAKALNTCISHISCVLIF 249 NOV2 254 YLPIINLAVVHRFAGHVSPLINVLMANVLLLVPPLMKPIVYCVKTKQIRVRVVAKL 309
  • SNP single nucleotide polymorphic
  • TTA 241 GCTGCTCCTTTTTGGATGCAATTCCCGGTACAGTCTTGAGGATCAGGGTGTA ⁇ GACACAGCAATGAGA ⁇ TAAAGTC
  • NOV2 expression was analyzed by TaqMan analysis. Results shown in Example 4, Table 15B demonstate that NOV2 expression is higher in lung cancer (small cell) as compared to normal tissue.
  • a NOV3 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins.
  • a NOV3 nucleic acid is found on human chromosome 11.
  • a NOV3 nucleic acid and its encoded polypeptide includes the sequences shown in Table 4A.
  • the disclosed nucleic acid (SEQ LD NO.: 5) is 980 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 25-27 and ends with a TAA stop codon at nucleotides 964-966.
  • a putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 4A, and the start and stop codons are in bold letters.
  • the representative ORF encodes a 313 amino acid polypeptide (SEQ ID NO.: 6) with a predicted molecular weight of 35255.9 Da.
  • PSORT analysis of aNOV3 polypeptide predicts aplasma membrane protein with a certainty of 0.6000. Using SIGNALP analysis, it is predicted that a NOV3 polypeptide has no signal peptide.
  • a NOV3 nucleic acid has homology (61% identity) to a Homo sapiens GPCR mRNA (GPCR; patn: T59274), as is shown in Table 4B.
  • a NOV3 polypeptide has homology (53% identity, 72%> similarity) with a 319 amino acid residue olfactory receptor-like protein from Gallus gallus (GALl; SPTREMBL-ACC: Q9YH55), as is shown in Table 4C. Additional homology (46% identity, 69% similarity) to a HOR 5'BETA3 polypeptide from Homo sapiens (HORl; SPTREMBL-ACC: Q9Y5P1) was also identified, as is shown in Table 4D.
  • NOV3 expression was analyzed by TaqMan analysis. The sequence is moderately expressed in many tissues; it is slightly higher in brain regions, especially cerebral cortex, indicating a utility as a marker to identify neuronal derived tissues and cells, as shown in Example 4 at Table 16B.
  • a NOV4 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins.
  • a NOV4 nucleic acid is found on human chromosome 11.
  • a NOV4 nucleic acid and its encoded polypeptide include the sequences shown in Table 5 A.
  • the disclosed nucleic acid (SEQ ID NO.: 7) is 952 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 6-8 and ends with a TAA stop codon at nucleotides 945-947.
  • a putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 5 A, and the start and stop codons are in bold letters.
  • the representative ORF encodes a 313 amino acid polypeptide (SEQ ID NO.: 8) with a predicted molecular weight of 35077.8 Da.
  • PSORT analysis of a NOV4 polypeptide predicts a plasma membrane protein with a certainty of 0.6000. Using SIGNALP analysis, it is predicted that a NOV4 polypeptide has no signal peptide.
  • a NOV4 nucleic acid was cloned according to the method in Example 2.
  • a NOV4 nucleic acid has homology (64% identity) to a Homo sapiens olfactory receptor mRNA (OLR2; GENBANK-ID: HPFH6OR/acc: X81445), as is shown in Table 5B.
  • a NOV4 polypeptide has homology (51% identity, 71% similarity) with an olfactory receptor- like protein from Gallus gallus (GALl ; SPTREMBL-ACC: Q9YH55), as is shown in Table 5C.
  • the global sequence homology (as defined by FASTA alignment with the full length sequence of this protein) is 49.518 % amino acid identity and 60.450 % amino acid similarity.
  • this protein contains the following protein domains: 7 transmembrane receptor (rhodopsin family) (IPR000276) from amino acid residues 3 to 222 (as defined by Interpro).
  • OLR2 1433 TCACCATGACGTTT-TCAA-ACGTTCCATAACATTCACACCTACAACATTCA-CTCTCGT 1489
  • OLR2 1490 TGGCATCCCGGGACTGGAGGCAGAACATTATGTGGATATCCATCCCCTTCTGCCTGATAT 1549
  • OLR2 1550 ACACCATCATCT-TTCCGGGAAATGGCATCATTCTTCACATCATCCGAATTGACTCTTCC 1608
  • OLR2 1609 TTGCACCAACCCATGTACTATTTTCTGGCCATGCCGGCCTTTGTTGAACTTGG-TGTCTC 1667 NOV4: 233 TTTATCATCTCTGCCCACTGTGTTAAGCATCTTCCTGTTCAATGCTCCTGAAACTTCATC 292
  • OLR2 1668 TGCTTCCACCATGCCCACTGTGTTAAGCATATTCCTCTTTGGCATTAACGATGTCAGTTT 1727
  • NOV4 293 CAATGCCTGCTTTGC-CCAGGAATTCTTC-ATTCATGGATTCTCAGTACTGGAGTCCTCA 350
  • OLR2 1728 TGGTGGTTGCCT-GCTCCAGATGTT-TTCTATGCACTCTTTCACTCTTATGGAGTCAGGT 1785
  • OLR2 1786 GTCCTTCTGGCAATGTCAGTGGACCGCTTTGTGGCCATCTACAGCCCACTGCGCTACACA 1845
  • NOV4 411 TCAATCCTGACAACTGTCAGAGTTGCCCAAATAGGGATAGT-ATTCTCCTTTAAGAGCAT 469
  • OLR2 1846 ACCATTCTGACAATTGCCTGCATTTCTGGGATGGGTGCCGCCATTG-CCTTGCGCAGTGT 1904
  • NOV4 470 GCTCCTGGTTCTTCC-CTTCCCTTTCACTTTAAGAAGCTTGAGATAT-TG-CAAGAAAAA 526
  • OLR2 1905 AATGCTTATGCTCCCACTGCTCTTTCTCCTGAGGCGTCTGCCTTTCTGTGGCCACAATAC 1964
  • NOV4 527 CCAATTATCCCATTCCTACTGTCTCCAC-CAGGATGTCATGAAGTTGGCCTGTTCTGACA 585
  • NOV4 586 ACA-GAATTGATGTTATCTATGGCTTT-TTTGG-AGCACTCTGC-CTTATGG—TAGACT 639
  • OLR2 2021 -CACGCCCCAATAGCATCC-TGGCTCTATTTGTCATTAC-CTTCACATTTGGACTGGACT 2077
  • NOV4 640 TTATTCT-CATTGCTGTGTCTTACACCCTGATCCTCAAGACTGTACCGGGAATTGCATCC 698
  • OLR2 2078 T-ATTGTTCATTGTGGTTTCTTATGTGCTGATTCTTCATACAGTACTGGAAATAGCTTCT 2136
  • NOV4 699 AAAA-AGGAGCAGCTTAAGGCTCTCAATACTTGTGTTTCACACATCTGTGCAGTGATCAT 757
  • OLR2 2137 GGAGCAGG-GC-GTGGCAGGCACTCAACACATGTGTGTCGCACATATGTGCTGTGCTTGT 2194
  • NOV4 758 CTTCTACCTGCCCATCATCAACCTGGCCGTTGTCCACCGCTTTGCCCGGCATGTCTCTCC 817
  • OLR2 2195 GTACTATGTGCCCATGATCAGCCTCTCC-TGATGCACCGCTTTGGACGGCATTTACCTCC 2253
  • NOV4 818 CCTCATTAATGTTCTCATGGCAAATGTTCTCCTACTTGTA-CCTCCGCTGATGAAACCAA 876
  • OLR2 2254 ACTTTTCCAGACTGTCACGGCCAATGCTTACCT-CTTCTTTCCTCCTGTGGTCAACCCCA 2312
  • OLR2 2313 TTGTCTATAGTATCAAAATCAAAGAAATTCGCAACAGCGTTGT 2355 (SEQ ID NO. 47)
  • GALl 12 FLLAGLPGMAQFHH VFLPFGLMYLVAVLGNGTILLVVRVHRQLHQPMYYFLLMLATTDL 71 N0V4 225 GLSLSSLPTVLSIFLFNAPETSSNACFAQEFFIHGFSVLESSVLLIMSFDRFLAIHNPLR 404
  • the possible novel SNP variants of NOV4 are shown in Table 5D.
  • the PHRED score indicates the SNP base pair quality, where a minimum score of 23 is acceptable.
  • the method of identifying and confirming novel SNPs is described in Example 3.
  • NOV4 expression was analyzed by TaqMan analysis. Results are shown in Example 4,
  • Table 14C demonstate that NOVl expression is higher in lung cancer (small cell), colon cancer, ovarian cancer, prostate cancer and uterine cancer as compared to normal tissue.
  • PCR probe/primer sets generated against GPCRs amplify the corresponding genomic region because GPCRs are single exon genes, as shown in Example 4 at Table 16B.
  • the sequence is moderately expressed in many tissues; it is slightly higher in brain regions, especially cerebral cortex, indicating a utility as a marker to identify neuronal derived tissues and cells.
  • a NOV5 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins.
  • a NOV5 nucleic acid is found on human chromosome 11.
  • a NOV5 nucleic acid and its encoded polypeptide include the sequences shown in Table 6A.
  • the disclosed nucleic acid (SEQ ID NO.: 9) is 885 nucleotides in length and contains an open reading frame (ORF) that begins with a GGG codon at nucleotides 2-4, which codes for the amino acid Glycine, and ends with a TAA stop codon at nucleotides 881-883.
  • a putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 6A, and the start and stop codons are in bold letters.
  • the representative ORF encodes a 293 amino acid polypeptide (SEQ ID NO.: 10).
  • PSORT analysis of a NOV5 polypeptide predicts a plasma membrane protein with a certainty of 0.6400.
  • SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 33 and 34 in SEQ LD NO.: 10.
  • the reverse complement (SEQ ID NO. 47) of a NOV5 nucleic acid sequence (SEQ LD NO. 9) has high homology (98% identity) with a nucleic acid encoding a human olfactory receptor-like polypeptide (HUMl ; : SeqCalling Accession No. : 150205708), as is shown in Table 6B.
  • HUMl human olfactory receptor-like polypeptide
  • a NOV5 polypeptide was found to have homology (52% identity, 72% similarity) to a Gallus gallus olfactory receptor-like protein COR3 ⁇ ETA (GALl; SPTREMBL-ACC: Q9YH55), as is shown in Table 6C.
  • NOV5 765 ACATTAATGAGGGGAGAGACATGCCGGGCAAAGCGGTGGACAACGGCCAGGTTGATGATG 706
  • HUMl 304 ATGAGAATAAAGTCTACCATAAGGCAGAGTGCTCCAA ⁇ A ⁇ A ⁇ GCCATAGATAACATCAAT 363
  • HUMl 424 TTGGTTTTTCTTGCAATATCTCAAGTTTCTTAAAGTGAA ⁇ GGGAAGGGAAGAACCAGGAG 483
  • HUMl 604 TGAGGACTCCAGTACTGAGAATCCATGAATGAAGAATTCCTGGGCAAAGCAGGCATTGGA 663
  • HUMl 724 CAAACCCAAGTCTGACATAGCCAACATGGAA ⁇ GAAAATAGTACATGGGCTCATGCAAGGA 783
  • HUMl 844 GCTGCAGATGGGGATAGAGATCCAGATGTGTGCATATTCTAGCCC 888 (SEQ ID NO. 51)
  • GALl (SEQ ID NO. 52)
  • a NOV6 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins.
  • a NOV6 nucleic acid is found on human chromosome 11.
  • a NOV6 nucleic acid and its encoded polypeptide include the sequences shown in Table 7A.
  • the disclosed nucleic acid (SEQ D NO.: 11) is 798 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 24-26 and ends with a TGA stop codon at nucleotides 780-782.
  • a putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 7A, and the start and stop codons are in bold letters.
  • the representative ORF encodes a 252 amino acid polypeptide (SEQ ID NO.: 12) with a predicted molecular weight of 28399.4 Da.
  • PSORT analysis of a NOV6 polypeptide predicts a plasma membrane protein with a certainty of 0.6400.
  • SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 61 and 62 in SEQ ID NO.: 12.
  • a NOV6 nucleic acid has homology (67% identity) with a Mus musculus GPCR mRNA (MUS3; GENBANK-LD: AF102539), as is shown in Table 7B.
  • a NOV6 polypeptide has homology (59% identity, 74% similarity) with a 223 amino acid residue olfactory receptor protein bom Mus musculus (OLR3; SPTREMBL-ACC: Q9Z1T8), as is shown in Table 7C.
  • this protein contains a seven transmembrane receptor domain (rhodopsin family) (LPR000276) from amino acid residues 1 to 232 (as defined by Interpro).
  • a NOV7 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins.
  • a NOV7 nucleic acid is found on human chromosome 11.
  • a NOV7 nucleic acid and its encoded polypeptide include the sequences shown in Table 8 A.
  • the disclosed nucleic acid (SEQ ID NO.: 13) is 995 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 21-23 and ends with a TAA stop codon at nucleotides 966-968.
  • a putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 8A, and the start and stop codons are in bold letters.
  • the representative ORF encodes a 315 amino acid polypeptide (SEQ ID NO.: 14) with a predicted molecular weight of 34934.2 Da.
  • PSORT analysis of a NOV7 polypeptide predicts a plasma membrane protein with a certainty of 0.6000.
  • SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 46 and 47 in SEQ ID NO.: 14.
  • a NOV7 nucleic acid has homology (38% identity) with a Pan tro lodytes GPCR mRNA (PAN1; GENBANK-ID: AF101730), as is shown in Table 8B.
  • ANOV7 polypeptide has homology (49% identity, 64% similarity) with an olfactory-like receptor protein from Homo sapiens (OLR3; SPTREMBL-ACC: CAB65799), as is shown in Table 8C.
  • this protein contains a seven transmembrane receptor domain (rhodopsin family) (LPR000276) from amino acid residues 40 to 149 (as defined by Interpro).
  • OLR3 361 GACCGCTATGTGGCCATCTGCTTCCCCCTGCACTACACCGCCATCATGAGCCCCATGCTC 420 NOV7 435 TGTGTC-CAGTTGATGGCTCTGTCCTGGCTCAACAGAGGGGCCTTGGGACTCTTGT-ACA 492
  • NOV7 expression was analyzed by TaqMan analysis. Results are shown in Example 4, Table 17B, 17C and 17E. Tissue expression of NOV7 (AC026038_B) is overall at a low level in many tissues, as shown in Example 4 at Table 17B. The highest expression is seen in testis. Thus, NOV7 (AC026038_B) may be used as a marker for male germ cells and may have therapeutic applications in fertility disorders as a potential target. NOV7 (AC026038_B) is overexpressed in tumors derived from tissues responsive to steroid hormones- ovarian, uterine and prostate cancers as shown in Example 4 at Table 17C (Panel 2D). It is therefore a marker for cells, especially tumor cells responsive to steroid hormones.
  • the protein can be used to screen candidate therapeutics for molecules able to modulate tumor growth, preferably small molecule therapeutic and human monoclonal antibodies.
  • Tables 17C and 17E Panel 4D shows that Agl201 may have a potential role in inflammation, since NOV7 is expressed in activated basophils. Basophils are one of the key cell mediators of inflammation during asthma and allergy (lfnmunopharmacolo y 2000 Jul 25;48(3):269-81.
  • Immunologically mediated signaling in basophils and mast cells finding therapeutic targets for allergic diseases in the human Fcvar epsilonRl signaling pathway. Oliver JM, Kepley CL, Ortega E, Wilson BS.). This molecule may be important in allowing these cells to extravasate into the site of inflammation and/or in the activation of these cells.
  • Antibody therapeutics to Agl201 may inhibit nasal and lung inflammation due to basophil activation and effectively reduce or eliminate symptoms of asthma, emphysema, and allergic rhinitis.
  • the high affinity IgE receptor, Fcvar epsilon RI plays key roles in an array of acute and chronic human allergic reactions including asthma, allergic rhinitis, atopic dermatitis, urticaria and anaphylaxis. In humans and rodents, this receptor is found at high levels on basophils and mast cells where its activation by IgE and multivalent antigen produces mediators and cytokines responsible for Fcvar epsilon Rl-dependent acute inflammation. Mast cells can additionally contribute to sustained inflammatory responses by internalizing antigen bound to IgE-Fcvar epsilon RI complexes for processing to peptides and presentation to T cells.
  • the Fcvar epsilon RI is also expressed, at lower density, on monocytes, macrophages and dendritic cells (DC), where its likely functions again include both signaling to mediator and cytokine production and antigen presentation.
  • DC dendritic cells
  • Antigen-stimulated Fcvar epsilon RI signaling may be limited by: (1) sequestering the Fcvar epsilon Rl-associated protein-tyrosine kinase, Lyn, that initiates FcvarepsilonRIsignaling; (2) eliminating; or (3) inactivating the protein-tyrosine kinase, Syk, that propagates FcvarepsilonRI signaling; and (4) establishing inhibitory crosstalk between Fcvar epsilon RI and a co-expressed receptor, FcgammaRII, that again limits Fc varepsilonRI- mediated Syk activation. These strategies may form the basis for new therapies for allergic inflammation.
  • a NOV8 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins.
  • a NOV8 nucleic acid is found on human chromosome 11.
  • a NOV8 nucleic acid and its encoded polypeptide include the sequences shown in Table 9A.
  • the disclosed nucleic acid (SEQ ID NO.: 15) is 969 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 27-29 and ends with a TAA stop codon at nucleotides 951-953.
  • a putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 9A, and the start and stop codons are in bold letters.
  • the representative ORF encodes a 308 amino acid polypeptide (SEQ LD NO.: 16) with a predicted molecular weight of 33960.9 Da.
  • PSORT analysis of a NOV8 polypeptide predicts a plasma membrane protein with a certainty of 0.6400.
  • SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 41 and 42 in SEQ LD NO.: 16.
  • a NOV8 nucleic acid has homology (71% identity) to a Rattus norvegicus GPCR mRNA (RAT1; GENBANK-ID: M64378) as is shown in Table 9B, and aNOV8 nucleic acid reverse complementary sequence has a high degree of homology (97% identity) to a nucleic acid encoding a human olfactory receptor-like polypeptide (HUM2; SeqCalling Accession No.:87740262) as is shown in Table 9C.
  • RAT1 Rattus norvegicus GPCR mRNA
  • HUM2 human olfactory receptor-like polypeptide
  • a NOV8 polypeptide has homology (68% identity, 79%o similarity) to an olfactory-like receptor protein from Rattus norvegicus (OLR4; SPTREMBL-ACC: O95007), as is shown in Table 9D.
  • the global sequence homology (as defined by GAP alignment with the full length sequence of this protein) is 67 % amino acid identity and 73 % amino acid similarity.
  • this protein contains a seven transmembrane receptor domain (rhodopsin family) (LPR000276) from amino acid residue 41 to 290 (as defined by Interpro).
  • I III I II I III II III II I III I II I I 11 II 1 1 I RAT1 420 TCCTGGGCTGGCGATGCGGTTGGCCCTGGGATCCTGGCTGTGTGGGTTTTCTG-CAATCA 478 NOV8 496 CAGTGCCCACAGCCCTCATCAGTGGCCTGTCCTTCTGTGGCCCCCGTGCCATCAACCACT 555
  • RATl 896 ATGTCAAGGAAG-CTCTGC-GCAGGACGGTGAAGGGGAAGTGA 936 (SEQ ID NO. 62)
  • NOV8 expression was analyzed by TaqMan analysis.
  • a NOV8 nucleic acid is expressed in several tumor cell lines, including ovarian carcinoma OVCAR-8, and lung cancer (non-small cell) HOP-62, indicating a utility as a marker to identify tumor cells, preferably from ovarian and lung tumors, as shown in Example 4 at Table 18B.
  • the protein can be used to screen for molecules able to modulate tumor growth, preferably small molecule therapeutics and human monoclonal antibodies.
  • the expression in the adipose is tainted by genomic contamination.
  • the expression in the testis indicates a utility to specifically identify male germ cells.
  • a NOV9 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins.
  • a NOV9 nucleic acid is found on human chromosome 11.
  • a NOV9 nucleic acid and its encoded polypeptide include the sequences shown in Table 10A.
  • the disclosed nucleic acid (SEQ ID NO.: 17) is 936 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 5-7 and ends with a TAA stop codon at nucleotides 929-931.
  • a putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 10A, and the start and stop codons are in bold letters.
  • the representative ORF encodes a 308 amino acid polypeptide (SEQ ID NO.: 18).
  • PSORT analysis of a NOV9 polypeptide predicts a plasma membrane protein with a certainty of 0.6400.
  • SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 41 and 42 in SEQ LD NO.: 18.
  • a NOV9 nucleic acid has a high degree of homology (98% identity) to a Gorilla gorilla olfactory receptor mRNA (GORl ; GENBANK-ID: AF179756
  • a NOV9 polypeptide has homology (68%> identity, 79% similarity) to an olfactory-like receptor protein from Rattus norvegicus (OLR5; SWISSPROT-ACC: P23267), as is shown in Table IOC.
  • this protein contains a seven transmembrane receptor domain (rhodopsin family) (1PR000276) from amino acid residues 41 to 290 (as defined by Interpro).
  • a NOV10 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins.
  • a NOV10 nucleic acid is found on human chromosome 11.
  • a NOV10 nucleic acid and its encoded polypeptide include the sequences shown in Table 11 A.
  • the disclosed nucleic acid (SEQ LD NO.: 19) is 971 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 17-19 and ends with a TGA stop codon at nucleotides 950-952.
  • a putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 11 A, and the start and stop codons are in bold letters.
  • the representative ORF encodes a 311 amino acid polypeptide (SEQ LD NO.: 20) with a predicted molecular weight of 35147.4 Da.
  • PSORT analysis of a NOV10 polypeptide predicts a plasma membrane protein with a certainty of 0.6000.
  • SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 59 and 60 in SEQ ID NO.: 20.
  • a NOV10 nucleic acid has homology (63% identity) to a Homo sapiens GPCR mRNA (GPCR2; GENBANK-LD: AL096770), as is shown in Table 1 IB.
  • a NOV10 polypeptide has homology (51% identity, 68% similarity) to an olfactory receptor-like protein from Homo sapiens (OLR3; SPTREMBL-ACC: CAB65799), as is shown in Table 1 lC.
  • the global sequence homology is 50.49% amino acid identity and 57.37% amino acid similarity.
  • this protein contains a seven transmembrane receptor domain (rhodopsin family) (LPR000276) from amino acid residues 47 to 296 (as defined by Interpro).
  • NOV10 779 AGAGTGTTACAACCACAAGATGTGGAATGCAGGTAGAAAATGTTTTTGATCTA-CCCTCC 721
  • GPCR2 81387 AGAAGGTGGCTACAAATAGGTGTGGTAGGCAGGTGGAGAA-GACCTTGGTCCGGCCCTCA 81445
  • NOV10 720 TTAGAAGGAATCCTCATGA-TGACAGAAAAAATGTACATGTAGGAGAGAGTAATT-ACAA 663
  • NOV10 662 CAAAGGAGATTA-T-CACAAGAC-TTGTACCAAAAACCATGACTCCAATCTCAATGGT-A 607 n i i I I I I I I m i m i i n i i i m u m i i i
  • GPCR2 81505 CAAAC-AGATAAATGCTGCAGACGTTGTGAATGCAGCCA-G—TGCAATCTCATTAATGA 81560
  • NOV10 606 ATTACTTTGGGGTCTAAGAGGCTTAGGAGCTGTGGAATATTACAGAAAAATTGACGTATT 547
  • NOV10 546 -CTATTGCGCCCACAGAATGGTAATGAGAATGTTGCTATCA-CATGCATGAATCCACAGA 489 n i ni m 11 mi mu m i 11 m mi 11 m m
  • GPCR2 81620 ACTCTCTT-CCCACAGAGAGGTATGGAGAA-GTTAATGGCAGCATGCATGAGCCCAGAGA 81677 NOV10: 488 TCACCCCACTGAGCCACGACATGGCCATCA-TCC-T-CAGACAGACACCTTGGTTTATGA 432 i mi i in i ni i i mi i i i mi ni i i n i
  • GPCR2 81678 GGCCCCCAGCAATCCAC-ACA-G-CTATCACTGCATGCCTACAGGCACGGGGATCCATAA 81734 NOV10: 431 TGACCTCATAATGTAAAGGCCGGCAGATGGCCATATAGCGGTCATAGGACATCACTGTAA 372
  • GPCR2 81735 TAGTCTCATAATGAAGTGGTTGACAGATTGCTGCGTACCTGTCATAAGACATCACTGTGA 81794
  • NOV10 371 GGATGGCTACCTCCGTAGTTGCCA-AGTCCATGAAAAAGAAGGCTTGCAGAGCACACCCA 313
  • NOV10 312 AAGAAAGAAATGGAAGTATCATGAGTCAAG-GAACTAACAATAGATTTTGGAATTGTGAC 254
  • NOV10 253 AGAGATGTAACAAAAATCTAAGAA-AGACAAGTTTCTCAAAAAGAAATACATTGGGGTTT 195 n i i i i i i i i m n n i i 1 1 i m i n i n m i i i n i
  • GPCR2 81913 AGAGATGAAGCAGAGGTCCA-GAAGAGAGAGGTGCTTTAAAAAGTAATACATGGGGGAAT 81971 NOV10: 194 GGAGGTGAACATCCAGAGTGGTAAGTATGATGATTAGGAGATTTCCTATGACAGCTGCCA 135 GPCR2 81972 GGACGACGGTCCACGGTAATGATGGTGATAATGAGGAGGTTGCCTGTCAAGGCCAGCA 82031 NOV10 134 GGTAAACTAGGAAGAATAGAGCAGCATGTACAATCTGAATATCCCAGGAATTCGAAAATC 75 i m i i m m i in in m n i i II i mi i GPCR2 82032 GGTATGTCACCAGAAATACCAATGCATGTAAAATCTGAAGCTTACGCTCATCAGAAAACC 82091 NOV10 74 CCATGAGGAAAAATTCAGCAACTTCAGT 47 (SEQ ID NO. 71) III I 11 I III
  • OLR3 3 NLTSMSGFLLMGFSDERKLQILHALVFLVTYLLALTGNLLIITIITVDRRLHSPMYYFLK 62
  • OLR3 63 HLSLLDLCFISVTVPQSIANSLMGNGYISLVQCILQVFFFIALASSEVAILTVMSYDRYA 122
  • OLR3 123 AICQPLHYETIMDPRACRHAVIAV IAGGLSGLMHAAINFSIPLCGKRVIHQFFCDVPQM 182
  • OLR3 183 LKLACSYEFINEIALAAFTTSAAFICLISIVLSYIRIFSTVLRIPSAEGRTKVFSTCLPH 242
  • NOV10 249 LVVVTLFMISGSIAYVKPISNSPPVLDVFLSAFYTWPPTLNPVIYSLRNRDMKAALRR 307
  • OLR3 243 LFVATFFLSAAGFEFLRLPSDSSSTVDLVFSVFYTVIPPTLNPVIYSLRNDSMKAALRK 301
  • NOV10 expression was analyzed by TaqMan analysis, as is shown in Table 19B.
  • a NOVl 1 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins.
  • a NOVl 1 nucleic acid is found on human chromosome 11.
  • a NOVl 1 nucleic acid and its encoded polypeptide include the sequences shown in Table 12A.
  • the disclosed nucleic acid (SEQ ID NO.: 21) is 1010 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 37-39 and ends with a TAA stop codon at nucleotides 982-984.
  • a putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 12A, and the start and stop codons are in bold letters.
  • the representative ORF encodes a 315 amino acid polypeptide (SEQ ID NO.: 22) with a predicted molecular weight of 35620.1 Da.
  • PSORT analysis of a NOVl 1 polypeptide predicts a plasma membrane protein with a certainty of 0.6000.
  • SIGNALP analysis suggests the presence of a signal peptide with the two possible cleavage sites: one occuring between positions 49 and 50, and one between positions 59 and 60 in SEQ LD NO.: 22.
  • a NOVl 1 nucleic acid has homology (88% identity) to a Homo sapiens GPCR mRNA (GPCR3; GENBANK-ID: AF065869), as is shown in Table 12B.
  • a NOVl 1 polypeptide has homology (69%> identity, 81% similarity) to an odorant receptor protein Rattus norvegicus (ODOR1; SPTREMBL-ACC: G264618), as is shown in Table 12C.
  • the global sequence homology (as defined by GAP alignment with the full length sequence of this protein) is 48% amino acid identity and 57% amino acid similarity.
  • this protein contains a seven transmembrane receptor domain (rhodopsin family) (LPR000276) from amino acid residues 39 to 285 (as defined by Interpro). TABLE 12B.
  • GPCR3 1 ATGAGACAGAATAATAATATTACAGAATTTGTCCTCCTGGGCTTCTCTCAGGATCTGGAT 60
  • GPCR3 61 GTGCAAAAAGCATTATTTGTCATATTTTTACTCACATACTTGGTGACAGTGGTGGGGAAC 120
  • GPCR3 121 CTGCTCATTGTGGTGACTATTATTACCAG-CCCTTCCTTGGGCTCCCCAATGTACTTCTT 179
  • NOVll 216 CCTTGCCTGCCTGTCATTTATAGATGCTGTGTATTCCACCACCATTTCTCCTGTATTGAT 275
  • GPCR3 180 CCTTGCCTGCCTGTCATTTATAGATGCTGCATATTCCACTACAATTTCTCCCAAATTGAT 239
  • GPCR3 240 TGTAGACTTACTCTGTGATAAAAAGACTATTTCTTTCCCAGCTTGCATGGGCCAGTTATT 299
  • NOVll 336 TATAGAGCACTTGTTTGGTGATACTGACGTCTTCCTTCTGGTGGTGATGGCCTATGATCG 395
  • GPCR3 300 TATATACCACTTGTTTGGTGGTTCTGAGGTCTTCCTTCTTGTGGTGATGGCCTGTGATCA 359
  • GPCR3 360 CTATGTGGCCATCTGTAAGCCACTGCACTATTTGACCATCATGAATCGACAGGTTTGAAT 419
  • GPCR3 420 CCTTCTGTTGGTGGTGGTCGTGACTGGAGGTTTTCTGCATTCTGTTTCAAATTGTTGT 479
  • GPCR3 480 TGTATACAGTCTCGCTTTCTGTGGCCCCAATGTCATTGACTACTTTGTCTGTGACATGTA 539
  • NOVll 576 CCCTTTATTGGACCTGGAATGCACTGACACCTACTTCGTAGGCCTCGCTGTGGTTTTCAA 635
  • GPCR3 540 CCCATTATTGGAACTGGTATGCACTGACACCTACTTTATTGGCCTTACTGTTTTTGTCAA 599
  • GPCR3 600 TGGTGGAACAATCTGTATAGTCGTCTTCACCCTTCTACTAATCTCCTATGGAGTCATCCT 659
  • GPCR3 660 AAACTCCCTTAAAACTTACAGTCAAGAAGGGAGGCATAAAG-TCCTGTTTACCTGCAGCT 718
  • GPCR3 719 CCCACATTATCGTCTTTGCCCTCTTTTTTGTTCCCTGTATTTTCATGTATGTTAGACCTG 778
  • NOVll 815 TTTCAAACTTTCCTATTGATAAATTCCTGACTGTGTTTTATTCAGTTATCACACCCAAGT 874
  • GPCR3 838 TGAATCCTTTAATATACACATTGAGAAATTCAGAGATGAGAAATTCTGTAGAAACACTCT 897
  • NOVll 1 MRQNNNITEFVLLGFSQYPDVQNALFVMFLLIYIVTMVGNLLIVVSIIASPFLGSPVYFF 60
  • ODOR1 1 MGENNNITEFILLGLTQDPDGRKALFVIFFLIYIVTMMGNLLIVVTVIASPSLGSPMYFF 60
  • ODOR1 61 LASLSLLDALFSTAISPKLIADLLYDQKTISFRACMSQLFIEHLFGGVDIVILVAMAYDR 120
  • NOVll 121 YVATCKPLRYLTIMNRQVCILLLWAVTGGFLHSVFQILVVYSLPFCGPNVIYHFFCNIY 180
  • ODOR1 121 YVAICKPLHYLAIMNRRVCITLLIFAWTGGFTHSLIQIVFVYNLPFCGPNVIDHFICDMS 180
  • ODOR1 181 PLLVLACTDTYFIGLTVIANGGVNCIVIFTLLLGSYGIILRSLKTQSQEGRRKALSTCSS 240
  • ODOR1 241 HILVVILFFVPCIFMYARPVYNFPIDKCITVFYTIITPMLNPLIYTLRNSEIKSCMKKL 299
  • ODOR1 (SEQ ID NO. 78)
  • a NOVl 2 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins.
  • a NOV12 nucleic acid is found on human chromosome 11.
  • a NOV12 nucleic acid and its encoded polypeptide include the sequences shown in Table 13A.
  • the disclosed nucleic acid (SEQ LD NO.: 23) is 988 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 23-25 and ends with a TAA stop codon at nucleotides 959-961.
  • a putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 13 A, and the start and stop codons are in bold letters.
  • the representative ORF encodes a 312 amino acid polypeptide (SEQ ID NO.: 24) with a predicted molecular weight of 35354.0 Da.
  • PSORT analysis of a NOV12 polypeptide predicts a plasma membrane protein with a certainty of 0.6000.
  • SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 38 and 39 in SEQ ID NO.: 24. TABLE 13A.
  • the reverse complement (SEQ LD NO. 75) of aNOV12 nucleic acid sequence (SEQ ID NO. 23) has homology (73% identity) to a Homo sapiens GPCR mRNA (GPCR4;
  • a NOV12 polypeptide has homology (48%o identity, 60% similarity) with a Mus musculus odorant receptor protein (ODOR2; SPTREMBL-ACC: BAA86125), as is shown in Table 13C.
  • the global sequence homology (as defined by GAP alignment with the full length sequence of this protein) is 48 % amino acid identity and 57 % amino acid similarity.
  • this protein contains a seven transmembrane receptor domain (rhodopsin family) (LPR000276) from amino acid residues 41 to 287 (as defined by Interpro).
  • NOVl 2 966 TAT-ATTTAAAAGATTTTACTGTAATTCACAAACTGAGAGCATCGTCTTCTCATTGCCAC 908
  • GPCR4 8393 TATCATTTATGAGATCTTCTTGTAAATCACTAGCTGTTTGCATACTCTCTTTATTGCTGC 8452
  • NOVl 2 907 CATCATCTCTTTATTTCTAAAAGTATAGATGACTGGATTCAAAACGGGAGTGATAACTGC 848
  • GPCR4 8453 CTTCATCTCCTTATTCCTGAATGTATAGACAACTGGATTCAGAAAAGGAGTGAGAACTGC 8512
  • NOVl 2 847 ATCA ⁇ AGATGGCAAGGAATTTATCAAGATGTGATGTGGGAAATGGAAAAATATAGAAA ⁇ 788
  • GPCR4 8513 ATCA ⁇ AAATAGCCAGAAACTTGTCCATCTGTGAATTAGGGTGTGGCCATGTATACACAAA 8572
  • NOV12 87 GATTAATGGCCCA ⁇ AGACCAAAACCACCACAATGACATGAGCTGACAGCATAGAGAAAGC 728
  • GPCR4 8573 CATGGGTGGACCAA ⁇ GA ⁇ CAAAAGGACCGCTGTGCTGTGAGCTGAAAGAGTGGA ⁇ AGGGC 8632
  • GPCR4 J633 CTTGGATGAACCACCTGAGGAATGTTTCCAAACAGTAAACAGGATGAAGATGTAGGAGAT 8692
  • NOVl 2 667 TATGAGAATTAAAAAAGAAGCCAGAGAAATAAATCCACTATTGGCAGTAACCATGAATCC 608
  • GPCR4 8693 TAGAAGTATGAAGAAAGTACCCACACAGATAAACCCACTGTTAACAGTGACCATGAACTG 8752
  • NOVl 2 607 CAATGTGTAGGTCTCTATGCAAGCCAGTTTGATAAATCGAGGAAGATCACAAAAGAAACT 548
  • GPCR4 8753 CAATCTGTAGGTGTCGGTACAGGCTAGTCTGAGAAGCTGAGGAAGGTCACAGTAGAAGCT 8812
  • NOVl 2 547 ATCTAATTCATTAGGGCCACAGAACAGCAGGTCTACAACAAAAGCCAACTGAATCACTGA 488
  • NOVl2 427 GATGGTCAGGTAGTGGAGAGGCTTACATATGGCCACATATCGGTCAAAAGCCATGGCTAT 368
  • GPCR4 8933 AATGGTCAGATAGTGGAGGGGCTTA-ATAGGGCCACATAACTGTCAAAGGCCATGGCTAT 8991
  • NOVl2 367 GAGCAGCACCATCTCAGTTCCCCCAACTG-CATGGATAAAGAAGATCTGAACTACACAGC 309
  • GPCR4 8992 GAGCAGCACCATCTCCACACCACCAA-TGACGTGGATGAAGAAGATTTGAGCGATGCAGC 9050
  • NOVl2 308 CCCCAAAAGAGATGGTCTTGTGCTTCCTGAAAAGGTCATAAATCATCTTGGGAGCTGTGG 249
  • GPCR4 9051 CTCCAAAGGAGATGACTTTGCGCTTTCTGAACAGGTCATAAATCATCTTGGGAGAAGTGA 9110
  • NOVl2 248 AGGAACAAAATACCAAATTGATGATGGAAAGGTTGGCCAGCAGGAAGTACATGGGGGACT 189
  • GPCR4 9111 CAGAGCAGGCTCCTAAGTCAATGAAGGAGAGACTGGCCAGTAGAAAGTACATGGGGGAGT 9170
  • NOVl2 188 GTAAACGAGGGTCAGAGGTCACAGTTAGCACAATGAGGAGATTTCCCATCAGGCTTGATA 129
  • GPCR4 9171 GTAAGTGAGGGTCAGTGGTCACAGAAAACACAATGAGGATGTTTCCAGTAATGCTTGCCA 9230
  • NOVl 2 128 CATAGAACACTGAGAAAAAGAGGAAGAGGAGGAGCTGGATCTTCCGCGAGTCAGAGAGTC 69 llllll III III I 11 I I I I II I I III II I I Ml I 1 II II I 11 II I I I
  • GPCR4 9231 CATAGAGCACAGAGGAAAACACTAGGAGGAGGAGCTGGATCTCCCATGAATGAGTGAGTC 9290
  • GPCR4 9291 CCAGAAACAAAAACTCAGATACCACTGAGTGATTCTCTCCATCCATTGGTCCAGCCAACT 9350
  • GPCR4 9351 GGG 9353 (SEQ ID NO. 80)
  • NOV12 1 MDEANHSVVSEFVFLGLSDSRKIQLLLFLFFSVFYVSSLMGNLLIVLTVTSDPRLQSPMY 60 I I + I+II+IIII+I+ +++I I+ I+I I+ +I+II III+I+ III +111
  • ODOR2 1 MGALNQTRVTEFIFLGLTDN VLEILFFVPFTVTYMLTLLGNFLIVVTIVFTPRLHNPMY 60
  • NOVl2 61 FLLANLSIINLVFCSSTAPKMIYDLFRKHKTISFGGCVVQIFFIHAVGGTEMVLLIAMAF 120
  • ODOR2 61 FFLSNLSFIDICHSSVTVPKMLEGLLLERKTISFDNCIAQLFFLHLFACSEIFLLTIMAY 120
  • NOVl2 121 DRYVAICKPLHYLTIMNPQRCILFLVISWIIGIIHSVIQLAFVVDLLFCGPNELDSFFCD 180 l llll l l lll l +11 + 1+ + I+ I IM++I + I +1111 +11 + 111
  • ODOR2 121 DRYVAICIPLHYSNVMNMKVCVQLVFAL LGGTIHSLVQTFLTIRLPYCGPNIIDSYFCD 180 NOVl2: 181 LPRFIKLACIETYTLGFMVTANSGFISLASFLILIISYIFILVTVQKKSSGGIFKAFSML 240
  • ODOR2 181 VPPVIKLACTDTYLTGILIVSNSGTISLVCFLALVTSYTVILFSLRKKSAEGRRKALSTC 240
  • NOVl2 241 SAHVIVWLVFGPLIFFYIFPFPTSHLDKFLAIFDAVITPVLNPVIYTFRNKEMMVAM— 298
  • ODOR2 241 SAHFMVVTLFFGPCIFLYTRPDSSFSIDKVVSVFYTVVTPLLNPLIYTLRNEEVKTAMKH 300
  • Novel SNP variants of NOV12 and their corresponding polypeptides are shown in Table 13D.
  • the method of identifying and confirming novel SNPs is described in Example 3.
  • GAG 561 GCTTACATATGGCCACATATCGGTCAAAAGCCATGGCTATGAGCAGCACCATCTCAGTTCCCCCAACTGCATGGATA AAG 641
  • NOVl 2 expression was analyzed by TaqMan analysis.
  • the expression of NOV12 in indicates highest levels in skeletal muscle and lower levels in the adipose tissue, as shown in Example 4 at Table 20B (Panel 1.2).
  • the expression in skeletal muscle could indicate that this GPCR may be involved in energy metabolism and regulation of either carbohydrate or lipid as energy sources. It therefore could be a potential small molecule target in the treatment of diabetes and/or obesity.
  • Polypeptides encoded by NOV1-NOV12 nucleic acids were analyzed by CLUSTALW analysis, as is shown in Table 21. TABLE 21.
  • NOV12 AFS LSAHVIWVLVFGPLIFFYIFP—FP—TSHLDKFLAIFDAVITPVLNPV1YTFRN
  • rOR indicates an olfactory-like receptor protein from Rattus norvegicus (SWISSPROT-ACC: P23267).
  • NOVl through NOV12 represent new members of the GPCR family.
  • NOVl through NOV12 can be used as markers for human chromosome 11.
  • NOVl through NOV12 are useful in determining changes in expression of genes contained within the GPCR protein family.
  • NOVl through NOV12 satisfy a need in the art by providing new diagnostic or therapeutic compositions useful in the treatment of disorders associated with alterations in the expression of members of the GPCR-associated protein families. Therefore, NOVl through NOV12, nucleic acids and proteins, and other compositions of the present invention identified here may be useful in potential therapeutic applications implicated in (but not limited to) various pathologies and disorders as indicated below.
  • the potential therapeutic applications for this invention include, but are not limited to: protein therapeutic, small molecule drug target, antibody target (therapeutic, diagnostic, drug targeting/cytotoxic antibody), diagnostic and/or prognostic marker, gene therapy (gene delivery/gene ablation), research tools, tissue regeneration in vivo and in vitro of all tissues and cell types composing (but not limited to) those defined here.
  • the nucleic acids and proteins of the invention are useful in potential therapeutic applications, including the treatment of infections such as bacterial, fungal, protozoal and viral infections (particularly infections caused by HIV-1 or HLV-2), pain, cancer (including but not limited to neoplasm, adenocarcinoma, lymphoma, prostate cancer, and uterine cancer), anorexia, bulimia, asthma, Parkinson's disease, acute heart failure, hypotension, hypertension, urinary retention, osteoporosis, Crohn's disease, multiple sclerosis, Albright hereditary ostoeodystrophy, angina pectoris, myocardial infarction, ulcers, asthma, allergies, benign prostatic hypertrophy, and psychotic and neurological disorders, including anxiety, schizophrenia, manic depression, delirium, dementia, severe mental retardation and dyskinesias, such as Huntington's disease or Gilles de la Tourette syndrome, and/or other pathologies and disorders.
  • infections such as bacterial, fungal, protozoal and viral
  • the polypeptides can be used as immunogens to produce antibodies specific for the invention, and as vaccines. They can also be used to screen for potential agonist and antagonist compounds.
  • a cDNA encoding the GPCR-like protein may be useful in gene therapy, and the GPCR-like protein may be useful when administered to a subject in need thereof.
  • compositions of the present invention will have efficacy for treatment of patients suffering from bacterial, fungal, protozoal and viral infections (particularly infections caused by HLV-1 or HIV-2), pain, cancer (including but not limited to neoplasm, adenocarcinoma, lymphoma, prostate cancer, and uterine cancer), anorexia, bulimia, asthma, Parkinson's disease, acute heart failure, hypotension, hypertension, urinary retention, osteoporosis, Crohn's disease, multiple sclerosis, Albright hereditary ostoeodystrophy, angina pectoris, myocardial infarction, ulcers, asthma, allergies, benign prostatic hypertrophy, and psychotic and neurological disorders, including anxiety, schizophrenia, manic depression, delirium, dementia, severe mental retardation and dyskinesias, such as Huntington's disease or Gilles de la Tourette syndrome, and/or other pathologies and disorders.
  • cancer including but not limited to neoplasm, adenocarcinoma, lymph
  • novel nucleic acid encoding GPCR-like protein, and the GPCR-like protein of the invention, or fragments thereof, may further be useful in diagnostic applications, wherein the presence or amount of the nucleic acid or the protein are to be assessed. These materials are further useful in the generation of antibodies that bind immunospecifically to the novel substances of the invention for use in therapeutic or diagnostic methods.
  • nucleic acids of the invention include those that encode a NOVX polypeptide or protein.
  • polypeptide and “protein” are interchangeable.
  • a NOVX nucleic acid encodes a mature NOVX polypeptide.
  • a "mature" form of a polypeptide or protein relates to the product of a naturally occurring polypeptide or precursor form or proprotein.
  • the naturally occurring polypeptide, precursor or proprotein includes, by way of nonlimiting example, the full-length gene product, encoded by the corresponding gene. Alternatively, it may be defined as the polypeptide, precursor or proprotein encoded by an open reading frame described herein.
  • the product "mature" form arises, again by way of nonlimiting example, as a result of one or more naturally occurring processing steps that may take place within the cell in which the gene product arises.
  • Examples of such processing steps leading to a "mature" form of a polypeptide or protein include the cleavage of the N-te ⁇ ninal methionine residue encoded by the initiation codon of an open reading frame, or the proteolytic cleavage of a signal peptide or leader sequence.
  • a mature form arising from a precursor polypeptide or protein that has residues 1 to N, where residue 1 is the N-terminal methionine would have residues 2 through N remaining after removal of the N-terminal methionine.
  • a mature form arising from a precursor polypeptide or protein having residues 1 to N, in which an N-terminal signal sequence from residue 1 to residue M is cleaved, would have the residues from residue M+l to residue N remaining.
  • a "mature" form of a polypeptide or protein may arise from a step of post-translational modification other than a proteolytic cleavage event. Such additional processes include, by way of non-limiting example, glycosylation, myristoylation or phosphorylation.
  • a mature polypeptide or protein may result from the operation of only one of these processes, or a combination of any of them.
  • NOVX nucleic acids is the nucleic acid whose sequence is provided in
  • SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or a fragment thereof includes mutant or variant nucleic acids of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or a fragment thereof, any of whose bases may be changed from the corresponding bases shown in SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, while still encoding a protein that maintains at least one of its NOVX-like activities and physiological functions.
  • the invention further includes the complement of the nucleic acid sequence of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, including fragments, derivatives, analogs and homologs thereof.
  • the invention additionally includes nucleic acids or nucleic acid fragments, or complements thereto, whose structures include chemical modifications.
  • nucleic acid molecules that encode NOVX proteins or biologically active portions thereof. Also included are nucleic acid fragments sufficient for use as hybridization probes to identify NOVX-encoding nucleic acids (e.g., NOVX mRNA) and fragments for use as polymerase chain reaction (PCR) primers for the amplification or mutation of NOVX nucleic acid molecules.
  • nucleic acid molecule is intended to include DNA molecules (e.g., cDNA or genomic DNA), RNA molecules (e.g., mRNA), analogs of the DNA or RNA generated using nucleotide analogs, and derivatives, fragments and homologs thereof.
  • the nucleic acid molecule can be single-stranded or double-stranded, but preferably is double-stranded DNA.
  • Probes refer to nucleic acid sequences of variable length, preferably between at least about 10 nucleotides (nt), 100 nt, or as many as about, e.g., 6,000 nt, depending on use. Probes are used in the detection of identical, similar, or complementary nucleic acid sequences. Longer length probes are usually obtained from a natural or recombinant source, are highly specific and much slower to hybridize than oligomers. Probes may be single- or double-stranded and designed to have specificity in PCR, membrane-based hybridization technologies, or ELISA-like technologies.
  • an "isolated" nucleic acid molecule is one that is separated from other nucleic acid molecules that are present in the natural source of the nucleic acid.
  • isolated nucleic acid molecules include, but are not limited to, recombinant DNA molecules contained in a vector, recombinant DNA molecules maintained in a heterologous host cell, partially or substantially purified nucleic acid molecules, and synthetic DNA or RNA molecules.
  • an "isolated" nucleic acid is free of sequences which naturally flank the nucleic acid (i.e., sequences located at the 5' and 3' ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived.
  • the isolated NOVX nucleic acid molecule can contain less than about 50 kb, 25 kb, 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb or 0.1 kb of nucleotide sequences which naturally flank the nucleic acid molecule in genomic DNA of the cell from which the nucleic acid is derived.
  • an "isolated" nucleic acid molecule such as a cDNA molecule, can be substantially free of other cellular material or culture medium when produced by recombinant techniques, or of chemical precursors or other chemicals when chemically synthesized.
  • a nucleic acid molecule of the present invention e.g., a nucleic acid molecule having the nucleotide sequence of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or a complement of any of this nucleotide sequence, can be isolated using standard molecular biology techniques and the sequence information provided herein.
  • NOVX nucleic acid sequences can be isolated using standard hybridization and cloning techniques (e.g., as described in Sambrook et ⁇ l, eds., MOLECULAR CLONING: A LABORATORY MANUAL 2 nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989; and Ausubel, et ⁇ l, eds., CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, New York, NY, 1993.)
  • a nucleic acid of the invention can be amplified using cDNA, mRNA or alternatively, genomic DNA, as a template and appropriate oligonucleotide primers according to standard PCR amplification techniques.
  • the nucleic acid so amplified can be cloned into an appropriate vector and characterized by DNA sequence analysis.
  • oligonucleotides corresponding to NOVX nucleotide sequences can be prepared by standard synthetic techniques, e.g., using an automated DNA synthesizer.
  • oligonucleotide refers to a series of linked nucleotide residues, which oligonucleotide has a sufficient number of nucleotide bases to be used in a PCR reaction.
  • a short oligonucleotide sequence may be based on, or designed from, a genomic or cDNA sequence and is used to amplify, confirm, or reveal the presence of an identical, similar or complementary DNA or RNA in a particular cell or tissue.
  • Oligonucleotides comprise portions of a nucleic acid sequence having about 10 nt, 50 nt, or 100 nt in length, preferably about 15 nt to 30 nt in length.
  • an oligonucleotide comprising a nucleic acid molecule less than 100 nt in length would further comprise at lease 6 contiguous nucleotides of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or a complement thereof. Oligonucleotides may be chemically synthesized and may be used as probes.
  • an isolated nucleic acid molecule of the invention comprises a nucleic acid molecule that is a complement of the nucleotide sequence shown in SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or a portion of this nucleotide sequence.
  • a nucleic acid molecule that is complementary to the nucleotide sequence shown in SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 is one that is sufficiently complementary to the nucleotide sequence shown in SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 that it can hydrogen bond with little or no mismatches to the nucleotide sequence shown in SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, thereby forming a stable duplex.
  • binding means the physical or chemical interaction between two polypeptides or compounds or associated polypeptides or compounds or combinations thereof. Binding includes ionic, non-ionic, Von der Waals, hydrophobic interactions, etc.
  • a physical interaction can be either direct or indirect. Indirect interactions may be through or due to the effects of another polypeptide or compound. Direct binding refers to interactions that do not take place through, or due to, the effect of another polypeptide or compound, but instead are without other substantial chemical intermediates.
  • nucleic acid molecule of the invention can comprise only a portion of the nucleic acid sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, e.g., a fragment that can be used as a probe or primer, or a fragment encoding a biologically active portion of NOVX. Fragments provided herein are defined as sequences of at least 6
  • (contiguous) nucleic acids or at least 4 (contiguous) amino acids a length sufficient to allow for specific hybridization in the case of nucleic acids or for specific recognition of an epitope in the case of amino acids, respectively, and are at most some portion less than a full length sequence.
  • Fragments may be derived from any contiguous portion of a nucleic acid or amino acid sequence of choice.
  • Derivatives are nucleic acid sequences or amino acid sequences formed from the native compounds either directly or by modification or partial substitution.
  • Analogs are nucleic acid sequences or amino acid sequences that have a structure similar to, but not identical to, the native compound but differs from it in respect to certain components or side chains. Analogs may be synthetic or from a different evolutionary origin and may have a similar or opposite metabolic activity compared to wild type.
  • Derivatives and analogs may be full length or other than full length, if the derivative or analog contains a modified nucleic acid or amino acid, as described below.
  • Derivatives or analogs of the nucleic acids or proteins of the invention include, but are not limited to, molecules comprising regions that are substantially homologous to the nucleic acids or proteins of the invention, in various embodiments, by at least about 70%, 80%, 85%, 90%, 95%o, 98%, or even 99% identity (with a preferred identity of 80-99%) over a nucleic acid or amino acid sequence of identical size or when compared to an aligned sequence in which the alignment is done by a computer homology program known in the art, or whose encoding nucleic acid is capable of hybridizing to the complement of a sequence encoding the aforementioned proteins under stringent, moderately stringent, or low stringent conditions.
  • a “homologous nucleic acid sequence” or “homologous amino acid sequence,” or variations thereof, refer to sequences characterized by a homology at the nucleotide level or amino acid level as discussed above.
  • Homologous nucleotide sequences encode those sequences coding for isoforms of a NOVX polypeptide. Isoforms can be expressed in different tissues of the same organism as a result of, for example, alternative splicing of RNA. Alternatively, isoforms can be encoded by different genes.
  • homologous nucleotide sequences include nucleotide sequences encoding for a NOVX polypeptide of species other than humans, including, but not limited to, mammals, and thus can include, e.g., mouse, rat, rabbit, dog, cat cow, horse, and other organisms.
  • homologous nucleotide sequences also include, but are not limited to, naturally occurring allelic variations and mutations of the nucleotide sequences set forth herein.
  • a homologous nucleotide sequence does not, however, include the nucleotide sequence encoding huma NOVX protein.
  • Homologous nucleic acid sequences include those nucleic acid sequences that encode conservative amino acid substitutions (see below) SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24, as well as a polypeptide having NOVX activity. Biological activities of the NOVX proteins are described below. A homologous amino acid sequence does not encode the amino acid sequence of a huma NOVX polypeptide.
  • the nucleotide sequence determined from the cloning of the huma NOVX gene allows for the generation of probes and primers designed for use in identifying and/or cloning NOVX homologues in other cell types, e.g., from other tissues, as well as NOVX homologues from other mammals.
  • the probe/primer typically comprises a substantially purified oligonucleotide.
  • the oligonucleotide typically comprises a region of nucleotide sequence that hybridizes under stringent conditions to at least about 12, 25, 50, 100, 150, 200, 250, 300, 350 or 400 or more consecutive sense strand nucleotide sequence of SEQ ED NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23; or an anti-sense strand nucleotide sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23; or of anaturally occurring mutant of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23.
  • Probes based on the human NOVX nucleotide sequence can be used to detect transcripts or genomic sequences encoding the same or homologous proteins.
  • the probe further comprises a label group attached thereto, e.g., the label group can be a radioisotope, a fluorescent compound, an enzyme, or an enzyme co-factor.
  • Such probes can be used as a part of a diagnostic test kit for identifying cells or tissue which misexpress a NOVX protein, such as by measuring a level of a NOVX-encoding nucleic acid in a sample of cells from a subject e.g., detecting NOVX mRNA levels or determining whether a genomic NOVX gene has been mutated or deleted.
  • polypeptide having a biologically active portion of NOVX refers to polypeptides exhibiting activity similar, but not necessarily identical to, an activity of a polypeptide of the present invention, including mature forms, as measured in a particular biological assay, with or without dose dependency.
  • a nucleic acid fragment encoding a "biologically active portion of NOVX” can be prepared by isolating a portion of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 that encodes a polypeptide having a NOVX biological activity (biological activities of the NOVX proteins are described below), expressing the encoded portion of NOVX protein (e.g., by recombinant expression in vitro) and assessing the activity of the encoded portion of NOVX.
  • a nucleic acid fragment encoding a biologically active portion of NOVX can optionally include an ATP -binding domain.
  • a nucleic acid fragment encoding a biologically active portion of NOVX includes one or more regions.
  • the invention further encompasses nucleic acid molecules that differ from the nucleotide sequences shown in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 due to the degeneracy of the genetic code.
  • These nucleic acids thus encode the same NOVX protein as that encoded by the nucleotide sequence shown in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 e.g., the polypeptide of SEQ D NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24.
  • an isolated nucleic acid molecule of the invention has a nucleotide sequence encoding a protein having an amino acid sequence shown SEQ ED NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24.
  • DNA sequence polymorphisms that lead to changes in the amino acid sequences of NOVX may exist within a population (e.g., the human population). Such genetic polymorphism in the NOVX gene may exist among individuals within a population due to natural allelic variation.
  • the terms "gene” and “recombinant gene” refer to nucleic acid molecules comprising an open reading frame encoding a NOVX protein, preferably a mammalia NOVX protein.
  • Such natural allelic variations can typically result in 1-5% variance in the nucleotide sequence of the NOVX gene. Any and all such nucleotide variations and resulting amino acid polymorphisms in NOVX that are the result of natural allelic variation and that do not alter the functional activity of NOVX are intended to be within the scope of the invention. Moreover, nucleic acid molecules encoding NOVX proteins from other species, and thus that have a nucleotide sequence that differs from the human sequence of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 are intended to be within the scope of the invention.
  • Nucleic acid molecules corresponding to natural allelic variants and homologues of the NOVX cDNAs of the invention can be isolated based on their homology to the huma NOVX nucleic acids disclosed herein using the human cDNAs, or a portion thereof, as a hybridization probe according to standard hybridization techniques under stringent hybridization conditions.
  • a soluble huma NOVX cDNA can be isolated based on its homology to human membrane-bound NOVX.
  • a membrane-bound huma NOVX cDNA can be isolated based on its homology to soluble huma NOVX.
  • an isolated nucleic acid molecule of the invention is at least 6 nucleotides in length and hybridizes under stringent conditions to the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23.
  • the nucleic acid is at least 10, 25, 50, 100, 250, 500 or 750 nucleotides in length.
  • an isolated nucleic acid molecule of the invention hybridizes to the coding region.
  • hybridizes under stringent conditions is intended to describe conditions for hybridization and washing under which nucleotide sequences at least 60% homologous to each other typically remain hybridized to each other.
  • Homologs i.e., nucleic acids encoding NOVX proteins derived from species other than human
  • other related sequences e.g., paralogs
  • stringent hybridization conditions refers to conditions under which a probe, primer or oligonucleotide will hybridize to its target sequence, but to no other sequences. Stringent conditions are sequence-dependent and will be different in different circumstances. Longer sequences hybridize specifically at higher temperatures than shorter sequences.
  • stringent conditions are selected to be about 5°C lower than the thermal melting point (T- for the specific sequence at a defined ionic strength and pH.
  • Tm is the temperature (under defined ionic strength, pH and nucleic acid concentration) at which 50% of the probes complementary to the target sequence hybridize to the target sequence at equilibrium. Since the target sequences are generally present at excess, at Tm, 50% of the probes are occupied at equilibrium.
  • stringent conditions will be those in which the salt concentration is less than about 1.0 M sodium ion, typically about 0.01 to 1.0 M sodium ion (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30°C for short probes, primers or oligonucleotides (e.g., 10 nt to 50 nt) and at least about 60°C for longer probes, primers and oligonucleotides.
  • Stringent conditions may also be achieved with the addition of destabilizing agents, such as formamide.
  • Stringent conditions are known to those skilled in the art and can be found in CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, NN. (1989), 6.3.1-6.3.6.
  • the conditions are such that sequences at least about 65%, 70%, 75%, 85%, 90%), 95%, 98%, or 99% homologous to each other typically remain hybridized to each other.
  • a non-limiting example of stringent hybridization conditions is hybridization in a high salt buffer comprising 6X SSC, 50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.02% BSA, and 500 mg/ml denatured salmon sperm D ⁇ A at 65°C. This hybridization is followed by one or more washes in 0.2X SSC, 0.01% BSA at 50°C.
  • An isolated nucleic acid molecule of the invention that hybridizes under stringent conditions to the sequence of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 corresponds to a naturally occurring nucleic acid molecule.
  • a "naturally-occurring" nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that occurs in nature (e.g., encodes a natural protein).
  • a nucleic acid sequence that is hybridizable to the nucleic acid molecule comprising the nucleotide sequence of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or fragments, analogs or derivatives thereof, under conditions of moderate stringency is provided.
  • moderate stringency hybridization conditions are hybridization in 6X SSC, 5X Denhardt's solution, 0.5% SDS and 100 mg/ml denatured salmon sperm DNA at 55°C, followed by one or more washes in IX SSC, 0.1 % SDS at 37°C.
  • Other conditions of moderate stringency that may be used are well known in the art. See, e.g., Ausubel et al.
  • nucleic acid that is hybridizable to the nucleic acid molecule comprising the nucleotide sequence of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or fragments, analogs or derivatives thereof, under conditions of low stringency, is provided.
  • low stringency hybridization conditions are hybridization in 35% formamide, 5X SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 mg/ml denatured salmon sperm DNA, 10% (wt/vol) dextran sulfate at 40°C, followed by one or more washes in 2X SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS at 50°C.
  • Other conditions of low stringency that may be used are well known in the art (e.g., as employed for cross-species hybridizations).
  • allelic variants of the NOVX sequence that may exist in the population, the skilled artisan will further appreciate that changes can be introduced by mutation into the nucleotide sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, thereby leading to changes in the amino acid sequence of the encoded NOVX protein, without altering the functional ability of the NOVX protein.
  • nucleotide substitutions leading to amino acid substitutions at "non-essential" amino acid residues can be made in the sequence of SEQ ED NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23.
  • a "non-essential" amino acid residue is a residue that can be altered from the wild-type sequence of NOVX without altering the biological activity, whereas an "essential" amino acid residue is required for biological activity.
  • amino acid residues that are conserved among the NOVX proteins of the present invention are predicted to be particularly unamenable to alteration.
  • Another aspect of the invention pertains to nucleic acid molecules encoding NOVX proteins that contain changes in amino acid residues that are not essential for activity. Such NOVX proteins differ in amino acid sequence from SEQ LD NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24, yet retain biological activity.
  • the isolated nucleic acid molecule comprises a nucleotide sequence encoding a protein, wherein the protein comprises an amino acid sequence at least about 75% homologous to the amino acid sequence of SEQ LD NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24 .
  • the protein encoded by the nucleic acid is at least about 80% homologous to SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24, more preferably at least about 90%, 95%, 98%, and most preferably at least about 99% homologous to SEQ LD NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24.
  • An isolated nucleic acid molecule encoding a NOVX protein homologous to the protein of can be created by introducing one or more nucleotide substitutions, additions or deletions into the nucleotide sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, such that one or more amino acid substitutions, additions or deletions are introduced into the encoded protein.
  • Mutations can be introduced into the nucleotide sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 by standard techniques, such as site-directed mutagenesis and PCR-mediated mutagenesis.
  • conservative amino acid substitutions are made at one or more predicted non-essential amino acid residues.
  • a "conservative amino acid substitution” is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art.
  • amino acids with basic side chains e.g., lysine, arginine, histidine
  • acidic side chains e.g., aspartic acid, glutamic acid
  • uncharged polar side chains e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine
  • nonpolar side chains e.g., alanine, valine, leucine, isoleucine, praline, phenylalanine, methionine, tryptophan
  • beta-branched side chains e.g., threonine, valine, isoleucine
  • aromatic side chains e.g., tyrosine, phenylalanine, tryptophan, histidine
  • a predicted nonessential amino acid residue in NOVX is replaced with another amino acid residue from the same side chain family.
  • mutations can be introduced randomly along all or part of a NOVX coding sequence, such as by saturation mutagenesis, and the resultant mutants can be screened for NOVX biological activity to identify mutants that retain activity.
  • the encoded protein can be expressed by any recombinant technology known in the art and the activity of the protein can be determined.
  • a mutant NOVX protein can be assayed for (1) the ability to form proteimprotein interactions with other NOVX proteins, other cell-surface proteins, or biologically active portions thereof, (2) complex formation between a mutant NOVX protein and a NOVX receptor; (3) the ability of a mutant NOVX protein to bind to an intracellular target protein or biologically active portion thereof; (e.g., avidin proteins); (4) the ability to bind NOVX protein; or (5) the ability to specifically bind an anti-NOVX protein antibody.
  • Another aspect of the invention pertains to isolated antisense nucleic acid molecules that are hybridizable to or complementary to the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or fragments, analogs or derivatives thereof.
  • An "antisense" nucleic acid comprises a nucleotide sequence that is complementary to a "sense" nucleic acid encoding a protein, e.g., complementary to the coding strand of a double-stranded cDNA molecule or complementary to an mRNA sequence.
  • antisense nucleic acid molecules comprise a sequence complementary to at least about 10, 25, 50, 100, 250 or 500 nucleotides or an entire NOVX coding strand, or to only a portion thereof.
  • Nucleic acid molecules encoding fragments, homologs, derivatives and analogs of a NOVX protein of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24 or antisense nucleic acids complementary to a NOVX nucleic acid sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 are additionally provided.
  • an antisense nucleic acid molecule is antisense to a "coding region" of the coding strand of a nucleotide sequence encoding NOVX.
  • coding region refers to the region of the nucleotide sequence comprising codons which are translated into amino acid residues (e.g., the protein coding region of huma NOVX corresponds to SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24).
  • the antisense nucleic acid molecule is antisense to a "noncoding region" of the coding strand of a nucleotide sequence encoding NOVX.
  • noncoding region refers to 5' and 3' sequences which flank the coding region that are not translated into amino acids (i.e., also referred to as 5' and 3' untranslated regions).
  • antisense nucleic acids of the invention can be designed according to the rules of Watson and Crick or Hoogsteen base pairing.
  • the antisense nucleic acid molecule can be complementary to the entire coding region of NOVX mRNA, but more preferably is an oligonucleotide that is antisense to only a portion of the coding or noncoding region of NOVX mRNA.
  • the antisense oligonucleotide can be complementary to the region surrounding the translation start site of NOVX mRNA.
  • An antisense oligonucleotide can be, for example, about 5, 10, 15, 20, 25, 30, 35, 40, 45 or 50 nucleotides in length.
  • An antisense nucleic acid of the invention can be constructed using chemical synthesis or enzymatic ligation reactions using procedures known in the art.
  • an antisense nucleic acid e.g., an antisense oligonucleotide
  • an antisense nucleic acid e.g., an antisense oligonucleotide
  • modified nucleotides that can be used to generate the antisense nucleic acid include: 5-fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4-acetylcytosine, 5-(carboxyhydroxylmethyl) uracil, 5-carboxymethylaminomethyl- 2-thiouridine, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-D-galactosylqueosine, inosine, N6-isopentenyladenine, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 5-methylcytosine, N6-adenine, 7-methylguanine, 5-methylaminomethyluracil, 5-methoxyaminomethyl-2-thiouracil, beta-D-mannosylqueosine, 5'-methoxy
  • the antisense nucleic acid can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation (i.e., RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest, described further in the following subsection).
  • the antisense nucleic acid molecules of the invention are typically administered to a subject or generated in situ such that they hybridize with or bind to cellular mRNA and/or genomic DNA encoding a NOVX protein to thereby inhibit expression of the protein, e.g., by inhibiting transcription and/or translation.
  • the hybridization can be by conventional nucleotide complementarity to form a stable duplex, or, for example, in the case of an antisense nucleic acid molecule that binds to DNA duplexes, through specific interactions in the major groove of the double helix.
  • An example of a route of administration of antisense nucleic acid molecules of the invention includes direct injection at a tissue site.
  • antisense nucleic acid molecules can be modified to target selected cells and then administered systemically.
  • antisense molecules can be modified such that they specifically bind to receptors or antigens expressed on a selected cell surface, e.g., by linking the antisense nucleic acid molecules to peptides or antibodies that bind to cell surface receptors or antigens.
  • the antisense nucleic acid molecules can also be delivered to cells using the vectors described herein. To achieve sufficient intracellular concentrations of antisense molecules, vector constructs in which the antisense nucleic acid molecule is placed under the control of a strong pol II or pol III promoter are preferred.
  • the antisense nucleic acid molecule of the invention is an ⁇ -anomeric nucleic acid molecule.
  • An ⁇ -anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual ⁇ -units, the strands run parallel to each other (Gaultier et al. (1987) Nucleic Acids Res 15: 6625-6641).
  • the antisense nucleic acid molecule can also comprise a 2'-o-methylribonucleotide (Inoue et al (1987) Nucleic Acids Res 15: 6131-6148) or a chimeric RNA -DNA analogue (Inoue et al (1987) FEBSLett 215: 327-330).
  • modifications include, by way of nonlimiting example, modified bases, and nucleic acids whose sugar phosphate backbones are modified or derivatized. These modifications are carried out at least in part to enhance the chemical stability of the modified nucleic acid, such that they may be used, for example, as antisense binding nucleic acids in therapeutic applications in a subject.
  • an antisense nucleic acid of the invention is a ribozyme.
  • Ribozymes are catalytic RNA molecules with ribonuclease activity that are capable of cleaving a single-stranded nucleic acid, such as a mRNA, to which they have a complementary region.
  • ribozymes e.g., hammerhead ribozymes (described in Haselhoff and Gerlach (1988) Nature 334:585-591)
  • a ribozyme having specificity for a NOVX-encoding nucleic acid can be designed based upon the nucleotide sequence of a NOVX DNA disclosed herein (i.e., SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23).
  • a derivative of a Tetrahymena L-19 IVS RNA can be constructed in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be cleaved in a NOVX-encoding mRNA. See, e.g., Cech et al. U.S. Pat. No. 4,987,071; and Cech et al. U.S. Pat. No. 5,116,742.
  • NOVX mRNA can be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA molecules. See, e.g., Bartel et al, (1993) -Science 261:1411-1418.
  • NOVX gene expression can be inhibited by targeting nucleotide sequences complementary to the regulatory region of the NOVX (e.g. , the NOVX promoter and/or enhancers) to form triple helical structures that prevent transcription of the NOVX gene in target cells.
  • nucleotide sequences complementary to the regulatory region of the NOVX e.g. , the NOVX promoter and/or enhancers
  • the nucleic acids of NOVX can be modified at the base moiety, sugar moiety or phosphate backbone to improve, e.g. , the stability, hybridization, or solubility of the molecule.
  • the deoxyribose phosphate backbone of the nucleic acids can be modified to generate peptide nucleic acids (see Hyrup et al. (1996) BioorgMed Chem 4: 5-23).
  • the terms "peptide nucleic acids” or "PNAs” refer to nucleic acid mimics, e.g., DNA mimics, in which the deoxyribose phosphate backbone is replaced by a pseudopeptide backbone and only the four natural nucleobases are retained.
  • PNAs The neutral backbone of PNAs has been shown to allow for specific hybridization to DNA and RNA under conditions of low ionic strength.
  • the synthesis of PNA oligomers can be performed using standard solid phase peptide synthesis protocols as described in Hyrup et al. (1996) above; Perry-O'Keefe et ⁇ /. (1996) EN S 93: 14670-675.
  • P ⁇ As of ⁇ OVX can be used in therapeutic and diagnostic applications. For example,
  • P ⁇ As can be used as antisense or antigene agents for sequence-specific modulation of gene expression by, e.g., inducing transcription or translation arrest or inhibiting replication.
  • P ⁇ As of ⁇ OVX can also be used, e.g., in the analysis of single base pair mutations in a gene by, e.g., P ⁇ A directed PCR clamping; as artificial restriction enzymes when used in combination with other enzymes, e.g., SI nucleases (Hyrup B. (1996) above); or as probes or primers for D ⁇ A sequence and hybridization (Hyrup et al. (1996), above; Perry-O'Keefe (1996), above).
  • P ⁇ As of ⁇ OVX can be modified, e.g., to enhance their stability or cellular uptake, by attaching lipophilic or other helper groups to P ⁇ A, by the formation of P ⁇ A-D ⁇ A chimeras, or by the use of liposomes or other techniques of drug delivery known in the art.
  • P ⁇ A-D ⁇ A chimeras of ⁇ OVX can be generated that may combine the advantageous properties of P ⁇ A and D ⁇ A.
  • Such chimeras allow D ⁇ A recognition enzymes, e.g., R ⁇ ase H and D ⁇ A polymerases, to interact with the D ⁇ A portion while the P ⁇ A portion would provide high binding affinity and specificity.
  • P ⁇ A-D ⁇ A chimeras can be linked using linkers of appropriate lengths selected in terms of base stacking, number of bonds between the nucleobases, and orientation (Hyrup (1996) above).
  • the synthesis of PNA-DNA chimeras can be performed as described in Hyrup (1996) above and Finn et al. (1996) Nucl Acids Res 24: 3357-63.
  • a DNA chain can be synthesized on a solid support using standard phosphoramidite coupling chemistry, and modified nucleoside analogs, e.g., 5'-(4-methoxytrityl) amino-5'-deoxy-thymidine phosphoramidite, can be used between the PNA and the 5' end of DNA (Mag et al. (1989) Nucl Acid Res 17: 5973-88). PNA monomers are then coupled in a stepwise manner to produce a chimeric molecule with a 5' PNA segment and a 3' DNA segment (Finn et al. (1996) above). Alternatively, chimeric molecules can be synthesized with a 5' DNA segment and a 3' PNA segment. See, Petersen et al. (1975) Bioorg Med Chem Lett 5: 1119-11124.
  • the oligonucleotide may include other appended groups such as peptides (e.g., for targeting host cell receptors in vivo), or agents facilitating transport across the cell membrane (see, e.g., Letsinger et al, 1989, Proc. Natl. Acad. Sci. U.S.A. 86:6553-6556; Lemaifre et al, 1987, Proc. Natl. Acad. Sci. 84:648-652; PCT Publication No. W088/09810) or the blood-brain barrier (see, e.g., PCT Publication No. W089/10134).
  • peptides e.g., for targeting host cell receptors in vivo
  • agents facilitating transport across the cell membrane see, e.g., Letsinger et al, 1989, Proc. Natl. Acad. Sci. U.S.A. 86:6553-6556; Lemaifre et al, 1987
  • oligonucleotides can be modified with hybridization triggered cleavage agents (See, e.g., Krol et al, 1988, BioTechniques 6:958-976) or intercalating agents. (See, e.g., Zon, 1988, Pharm. Res. 5: 539-549).
  • the oligonucleotide may be conjugated to another molecule, e.g., a peptide, a hybridization triggered cross-linking agent, a transport agent, a hybridization-triggered cleavage agent, etc.
  • a NOVX polypeptide of the invention includes the NOVX-like protein whose sequence is provided SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24.
  • the invention also includes a mutant or variant protein any of whose residues may be changed from the corresponding residue shown SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24 while still encoding a protein that maintains its NOVX-like activities and physiological functions, or a functional fragment thereof. In some embodiments, up to 20% or more of the residues may be so changed in the mutant or variant protein, hi some embodiments, the NOVX polypeptide according to the invention is a mature polypeptide.
  • a NOVX -like variant that preserves NOVX-like function includes any variant in which residues at a particular position in the sequence have been substituted by other amino acids, and further include the possibility of inserting an additional residue or residues between two residues of the parent protein as well as the possibility of deleting one or more residues from the parent sequence.
  • Any amino acid substitution, insertion, or deletion is encompassed by the invention. In favorable circumstances, the substitution is a conservative substitution as defined above.
  • One aspect of the invention pertains to isolated NOVX proteins, and biologically active portions thereof, or derivatives, fragments, analogs or homologs thereof. Also provided are polypeptide fragments suitable for use as immunogens to raise anti-NOVX antibodies.
  • native NOVX proteins can be isolated from cells or tissue sources by an appropriate purification scheme using standard protein purification techniques.
  • NOVX proteins are produced by recombinant DNA techniques.
  • a NOVX protein or polypeptide can be synthesized chemically using standard peptide synthesis techniques.
  • an “isolated” or “purified” protein or biologically active portion thereof is substantially free of cellular material or other contaminating proteins from the cell or tissue source from which the NOVX protein is derived, or substantially free from chemical precursors or other chemicals when chemically synthesized.
  • the language “substantially free of cellular material” includes preparations of NOVX protein in which the protein is separated from cellular components of the cells from which it is isolated or recombinantly produced.
  • the language "substantially free of cellular material” includes preparations of NOVX protein having less than about 30% (by dry weight) of non-NOVX protein (also referred to herein as a "contaminating protein"), more preferably less than about 20% of non-NOVX protein, still more preferably less than about 10% of non-NOVX protein, and most preferably less than about 5% non-NOVX protein.
  • non-NOVX protein also referred to herein as a "contaminating protein”
  • NOVX protein or biologically active portion thereof is recombinantly produced, it is also preferably substantially free of culture medium, i.e., culture medium represents less than about 20%, more preferably less than about 10%, and most preferably less than about 5% of the volume of the protein preparation.
  • the language “substantially free of chemical precursors or other chemicals” includes preparations of NOVX protein in which the protein is separated from chemical precursors or other chemicals that are involved in the synthesis of the protein.
  • the language “substantially free of chemical precursors or other chemicals” includes preparations of NOVX protein having less than about 30% (by dry weight) of chemical precursors or non-NOVX chemicals, more preferably less than about 20%o chemical precursors or non-NOVX chemicals, still more preferably less than about 10% chemical precursors or non-NOVX chemicals, and most preferably less than about 5%> chemical precursors or non-NOVX chemicals.
  • Biologically active portions of a NOVX protein include peptides comprising amino acid sequences sufficiently homologous to or derived from the amino acid sequence of the NOVX protein, e.g., the amino acid sequence shown SEQ LD NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24 that include fewer amino acids than the full length NOVX proteins, and exhibit at least one activity of a NOVX protein.
  • biologically active portions comprise a domain or motif with at least one activity of the NOVX protein.
  • a biologically active portion of a NOVX protein can be a polypeptide which is, for example, 10, 25, 50, 100 or more amino acids in length.
  • a biologically active portion of a NOVX protein of the present invention may contain at least one of the above-identified domains conserved between the NOVX proteins, e.g. TSR modules.
  • other biologically active portions, in which other regions of the protein are deleted can be prepared by recombinant techniques and evaluated for one or more of the functional activities of a native NOVX protein.

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Abstract

Disclosed herein are nucleic acid sequences that encode G-coupled protein-receptor related polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies, which immunospecifically-bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The invention further discloses therapeutic, diagnostic and research methods for diagnosis, treatment, and prevention of disorders involving any one of these novel human nucleic acids and proteins.

Description

NOVEL GPCR-PROTEINS AND NUCLEIC ACIDS ENCODING SAME
FIELD OF THE INVENTION
The present invention generally relates to novel proteins encoded by genomic DNA sequences and proteins similar to them, namely, new proteins bearing sequence similarity to G-protein coupled receptors (GPCRs), nucleic acids that encode these proteins or fragments thereof, and antibodies that bind immunospecifically to a protein of the invention.
BACKGROUND OF THE INVENTION
Olfactory receptors (ORs) have been identified as an extremely large subfamily of GPCRs in a number of species. These receptors share a seven transmembrane domain structure with many neurotransmitter and hormone receptors, and are likely to underlie the recognition of G-protein-mediated transduction of odorant signals. Previously, OR genes cloned in different species were from random locations in the respective genomes. The human OR genes are intron-less and belong to four different gene subfamilies, displaying great sequence variability. These genes are predominantly expressed in olfactory epithelium.
SUMMARY OF THE INVENTION
The invention is based, in part, upon the discovery of novel polynucleotide sequences encoding novel polypeptides.
Accordingly, in one aspect, the invention provides an isolated nucleic acid molecule that includes the sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 or a fragment, homolog, analog or derivative thereof. The nucleic acid can include, e.g., a nucleic acid sequence encoding a polypeptide at least 85% identical to a polypeptide that includes the amino acid sequences of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24. The nucleic acid can be, e.g., a genomic DNA fragment, or a cDNA molecule.
Also included in the invention is a vector containing one or more of the nucleic acids described herein, and a cell containing the vectors or nucleic acids described herein.
The invention is also directed to host cells transformed with a vector comprising any of the nucleic acid molecules described above.
In another aspect, the invention includes a pharmaceutical composition that includes a NOVX nucleic acid and a pharmaceutically acceptable carrier or diluent. In a further aspect, the invention includes a substantially purified NOVX polypeptide, e.g., any of the NOVX polypeptides encoded by a NOVX nucleic acid, and fragments, homologs, analogs, and derivatives thereof. The invention also includes a pharmaceutical composition that includes a NOVX polypeptide and a pharmaceutically acceptable carrier or diluent.
In still a further aspect, the invention provides an antibody that binds specifically to a NOVX polypeptide. The antibody can be, e.g., a monoclonal or polyclonal antibody, and fragments, homologs, analogs, and derivatives thereof. The invention also includes a pharmaceutical composition including NOVX antibody and a pharmaceutically acceptable carrier or diluent. The invention is also directed to isolated antibodies that bind to an epitope on a polypeptide encoded by any of the nucleic acid molecules described above.
The invention also includes kits comprising any of the pharmaceutical compositions described above.
The invention further provides a method for producing a NOVX polypeptide by providing a cell containing a NOVX nucleic acid, e.g., a vector that includes a NOVX nucleic acid, and culturing the cell under conditions sufficient to express the NOVX polypeptide encoded by the nucleic acid. The expressed NOVX polypeptide is then recovered from the cell. Preferably, the cell produces little or no endogenous NOVX polypeptide. The cell can be, e.g., aprokaryotic cell or eukaryotic cell. The invention is also directed to methods of identifying a NOVX polypeptide or nucleic acid in a sample by contacting the sample with a compound that specifically binds to the polypeptide or nucleic acid, and detecting complex formation, if present.
The invention further provides methods of identifying a compound that modulates the activity of a NOVX polypeptide by contacting a NOVX polypeptide with a compound and determining whether the NOVX polypeptide activity is modified.
The invention is also directed to compounds that modulate NOVX polypeptide activity identified by contacting a NOVX polypeptide with the compound and determining whether the compound modifies activity of the NOVX polypeptide, binds to the NOVX polypeptide, or binds to a nucleic acid molecule encoding a NOVX polypeptide. In another aspect, the invention provides a method of determining the presence of or predisposition of a NOVX-associated disorder in a subject. The method includes providing a sample from the subject and measuring the amount of NOVX polypeptide in the subject sample. The amount of NOVX polypeptide in the subject sample is then compared to the amount of NOVX polypeptide in a control sample. An alteration in the amount of NOVX polypeptide in the subject protein sample relative to the amount of NOVX polypeptide in the control protein sample indicates the subject has a tissue proliferation-associated condition. A control sample is preferably taken from a matched individual, i.e., an individual of similar age, sex, or other general condition, but who is not suspected of having a tissue proliferation- associated condition. Alternatively, the control sample may be taken from the subject at a time when the subject is not suspected of having a tissue proliferation-associated disorder. In some embodiments, the NOVX is detected using a NOVX antibody. hi a further aspect, the invention provides a method of determining the presence of or predisposition of a NOVX-associated disorder in a subj ect. The method includes providing a nucleic acid sample, e.g., RNA or DNA, or both, from the subject and measuring the amount of the NOVX nucleic acid in the subject nucleic acid sample. The amount of NOVX nucleic acid sample in the subject nucleic acid is then compared to the amount of a NOVX nucleic acid in a control sample. An alteration in the amount of NOVX nucleic acid in the sample relative to the amount of NOVX in the control sample indicates the subject has a NOVX- associated disorder.
In a still further aspect, the invention provides a method of treating or preventing or delaying a NOVX-associated disorder. The method includes administering to a subject in which such treatment or prevention or delay is desired a NOVX nucleic acid, a NOVX polypeptide, or a NOVX antibody in an amount sufficient to treat, prevent, or delay a NOVX- associated disorder in the subject.
Also, NOV1-12 are homologous to members of the odorant receptor (OR) family of the human G-protein coupled receptor (GPCR) superfamily of proteins, as shown in Table 56. Thus, the NOV1-12 nucleic acids and polypeptides, antibodies and related compounds according to the invention will be useful in therapeutic and diagnostic applications in disorders of olfactory loss, e.g., trauma, HIV illness, neoplastic growth and neurological disorders e.g. Parkinson's disease and Alzheimer's disease.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In the case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.
Other features and advantages of the invention will be apparent from the following detailed description and claims.
DETAILED DESCRIPTION OF THE INVENTION
Olfactory receptors (ORs) are the largest family of G-protein-coupled receptors
(GPCRs) and belong to the first family (Class A) of GPCRs, along with catecholamine receptors and opsins. The OR family contains over 1,000 members that traverse the phylogenetic spectrum from C. elegans to mammals. ORs most likely emerged from prototypic GPCRs several times independently, extending the structural diversity necessary both within and between species in order to differentiate the multitude of ligands. Individual olfactory sensory neurons are predicted to express a single, or at most a few, ORs. All ORs are believed to contain seven α-helices separated by three extracellular and three cytoplasmic loops, with an extracellular amino-terminus and a cytoplasmic carboxy-terminus. The pocket of OR ligand binding is expected to be between the second and sixth transmembrane domains of the proteins. Overall amino acid sequence identity within the mammalian OR family ranges from 45% to >80%, and genes greater than 80% identical to one another at the amino acid level are considered to belong to the same subfamily. Since the first ORs were cloned in 1991, outstanding progress has been made into their mechanisms of action and potential dysregulation during disease and disorder. It is understood that some human diseases result from rare mutations within GPCRs. Drug discovery avenues could be used to produce highly specific compounds on the basis of minute structural differences of OR subtypes, which are now being appreciated with in vivo manipulation of OR levels in transgenic and knock-out animals. Furthermore, due to the intracellular homogeneity and ligand specificity of ORs, renewal of specific odorant-sensing neurons lost in disease or disorder is possible by the introduction of individual ORs into basal cells. Additionally, new therapeutic strategies may be elucidated by further study of so-called orphan receptors, whose ligand(s) remain to be discovered. OR proteins bind odorant ligands and transmit a G-protein-mediated intracellular signal, resulting in generation of an action potential. The accumulation of DNA sequences of hundreds of OR genes provides an opportunity to predict features related to their structure, function and evolutionary diversification. See Pilpel Y, et.al., Essays Biochem 1998;33:93- 104. The OR repertoire has evolved a variable ligand-binding site that ascertains recognition of multiple odorants, coupled to constant regions that mediate the cAMP-mediated signal transduction. The cellular second messenger underlies the responses to diverse odorants through the direct gating of olfactory-specific cation channels. This situation necessitates a mechanism of cellular exclusion, whereby each sensory neuron expresses only one receptor type, which in turn influences axonal projections. A 'synaptic image' of the OR repertoire thus encodes the detected odorant in the central nervous system.
The ability to distinguish different odors depends on a large number of different odorant receptors (ORs). ORs are expressed by nasal olfactory sensory neurons, and each neuron expresses only 1 allele of a single OR gene. In the nose, different sets of ORs are expressed in distinct spatial zones. Neurons that express the same OR gene are located in the same zone; however, in that zone they are randomly interspersed with neurons expressing other ORs. When the cell chooses an OR gene for expression, it may be restricted to a specific zonal gene set, but it may select from that set by a stochastic mechanism. Proposed models of OR gene choice fall into 2 classes: locus-dependent and locus-independent. Locus-dependent models posit that OR genes are clustered in the genome, perhaps with members of different zonal gene sets clustered at distinct loci. In contrast, locus-independent models do not require that OR genes be clustered. OR genes have been mapped to 11 different regions on 7 chromosomes. These loci lie within paralogous chromosomal regions that appear to have arisen by duplications of large chromosomal domains followed by extensive gene duplication and divergence. Studies have shown that OR genes expressed in the same zone map to numerous loci; moreover, a single locus can contain genes expressed in different zones. These findings raised the possibility that OR gene choice is locus-independent or involved consecutive stochastic choices. Issel-Tarver and Rine (1996) characterized 4 members of the canine olfactory receptor gene family. The 4 subfamilies comprised genes expressed exclusively in olfactory epithelium. Analysis of large DNA fragments using Southern blots of pulsed field gels indicated that subfamily members were clustered together, and that two of the subfamilies were closely linked in the dog genome. Analysis of the four olfactory receptor gene subfamilies in 26 breeds of dog provided evidence that the number of genes per subfamily was stable in spite of differential selection on the basis of olfactory acuity in scent hounds, sight hounds, and toy breeds. Issel-Tarver and Rine (1997) performed a comparative study of four subfamilies of olfactory receptor genes first identified in the dog to assess changes in the gene family during mammalian evolution, and to begin linking the dog genetic map to that of humans. These four families were designated by them OLF1, OLF2, OLF3, and OLF4 in the canine genome. The subfamilies represented by these four genes range in size from 2 to 20 genes. They are all expressed in canine olfactory epithelium but were not detectably expressed in canine lung, liver, ovary, spleen, testis, or tongue. The OLF1 and OLF2 subfamilies are tightly linked in the dog genome and also in the human genome. The smallest family is represented by the canine OLF1 gene. Using dog gene probes individually to hybridize to Southern blots of genomic DNA from 24 somatic cell hybrid lines. They showed that the human homologous OLF1 subfamily maps to human chromosome 11. The human gene with the strongest similarity to the canine OLF2 gene also mapped to chromosome 11. Both members of the human subfamily that hybridized to canine OLF3 were located on chromosome 7. It was difficult to determine to which chromosome or chromosomes the human genes that hybridized to the canine OLF4 probe mapped. This subfamily is large in mouse and hamster as well as human, so the rodent background largely obscured the human cross-hybridizing bands. It was possible, however, to discern some human-specific bands in blots corresponding to human chromosome 19. They refined the mapping of the human OLF1 homolog by hybridization to YACs that map to llqll. In dogs, the OLF1 and OLF2 subfamilies are within 45 kb of one another (Issel-Tarver and Rine (1996)).
Issel-Tarver and Rine (1997) demonstrated that in the human OLF1 and OLF2 homologs are likewise closely linked. By studying YACs, Issel-Tarver and Rine (1997) found that the human OLF3 homolog maps to 7q35. A chromosome 19-specific cosmid library was screened by hybridization with the canine OLF4 gene probe, and clones that hybridized strongly to the probe even at high stringency were localized to 19pl3.1 and 19pl3.2. These clones accounted, however, for a small fraction of the homologous human bands. Rouquier et al. (1998) demonstrated that members of the olfactory receptor gene family are distributed on all but a few human chromosomes. Through fluorescence in situ hybridization analysis, they showed that OR sequences reside at more than 25 locations in the human genome. Their distribution was biased for terminal bands of chromosome arms. Flow-sorted chromosomes were used to isolate 87 OR sequences derived from 16 chromosomes. Their sequence relationships indicated the inter- and intrachromosomal duplications responsible for OR family expansion. Rouquier et al. (1998) determined that the human genome has accumulated a striking number of dysfunctional copies: 72% of these sequences were found to be pseudogenes. ORF-containing sequences predominate on chromosomes 7, 16, and 17.
Trask et al. (1998) characterized a subtelomeric DNA duplication that provided insight into the variability, complexity, and evolutionary history of that unusual region of the human genome, the telomere. Using a DNA segment cloned from chromosome 19, they demonstrated that the blocks of DNA sequence shared by different chromosomes can be very large and highly similar. Three chromosomes appeared to have contained the sequence before humans migrated around the world. In contrast to its multicopy distribution in humans, this subtelomeric block maps predominantly to a single locus in chimpanzee and gorilla, that site being nonorthologous to any of the locations in the human genome. Three new members of the olfactory receptor (OR) gene family were found to be duplicated within this large segment of DNA, which was found to be present at 3q, 15q, and 19p in each of 45 unrelated humans sampled from various populations. From its sequence, one of the OR genes in this duplicated block appeared to be potentially functional. The findings raised the possibility that functional diversity in the OR family is generated in part through duplications and interchromosomal rearrangements of the DNA near human telomeres.
Mombaerts (1999) reviewed the molecular biology of the odorant receptor (OR) genes in vertebrates. Buck and Axel (1991) discovered this large family of genes encoding putative odorant receptor genes. Zhao et al. (1998) provided functional proof that one OR gene encodes a receptor for odorants. The isolation of OR genes from the rat by Buck and Axel (1991) was based on three assumptions. First, ORs are likely G protein-coupled receptors, which characteristically are 7-transmembrane proteins. Second, ORs are likely members of a multigene family of considerable size, because an immense number of chemicals with vastly different structures can be detected and discriminated by the vertebrate olfactory system. Third, ORs are likely expressed selectively in olfactory sensory neurons. Ben-Arie et al.
(1994) focused attention on a cluster of human OR genes on 17p, to which the first human OR gene, OR1D2, had been mapped by Schurmans et al. (1993). According to Mombaerts (1999), the sequences of more than 150 human OR clones had been reported.
The human OR genes differ markedly from their counterparts in other species by their high frequency of pseudogenes, except the testicular OR genes. Research showed that individual olfactory sensory neurons express a small subset of the OR repertoire. In rat and mouse, axons of neurons expressing the same OR converge onto defined glomeruli in the olfactory bulb. The present invention generally relates to novel proteins encoded by genomic DNA sequences and proteins similar to them, namely, new proteins bearing sequence similarity to GPCRs, nucleic acids that encode these proteins or fragments thereof, and antibodies that bind immunospecifically to a protein of the invention. Included in the invention are the novel nucleic acid sequences and their polypeptides. The sequences are collectively referred to as "NOVX nucleic acids" or "NOVX polynucleotides" and the corresponding encoded polypeptides are referred to as "NOVX polypeptides" or "NOVX proteins." Unless indicated otherwise, "NOVX" is meant to refer to any of the novel sequences disclosed herein. Table 1 provides a summary of the NOVX nucleic acids and their encoded polypeptides. Example 1 provides a description of how the novel nucleic acids were identified.
TABLE 1. Sequences and Corresponding SEQ ID Numbers
NOVX nucleic acids and their encoded polypeptides are useful in a variety of applications and contexts. The various NOVX nucleic acids and polypeptides according to the invention are useful as novel members of the protein families according to the presence of domains and sequence relatedness to previously described proteins. Additionally, NOVX nucleic acids and polypeptides can also be used to identify proteins that are members of the family to which the NOVX polypeptides belong.
For example, NOV1 through NOV12 are homologous to members of the GPCR family of proteins that are important in maintaining G-protein mediated signal transduction. Thus, the NOV1 nucleic acids, polypeptides, antibodies and related compounds according to the invention will be useful in therapeutic and diagnostic applications in disorders characterized by G-protein signaling, e.g., bacterial, fungal, protozoal and viral infections, pain, cancer, anorexia, bulimia, asthma, and Parkinson's disease.
The NOVX nucleic acids and polypeptides can also be used as immunogens to produce antibodies specific to the invention, and to screen for molecules, which inhibit or enhance NOVX activity or function.
Additional utilities for the NOVX nucleic acids and polypeptides according to the invention are disclosed herein.
NOVl
A NOVl sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins. A NOVl nucleic acid is found on human chromosome 11. A NOVl nucleic acid and its encoded polypeptide includes the sequences shown in Table 2A. The disclosed nucleic acid (SEQ ID NO.: 1) is 978 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 21-23 and ends with a TAA stop codon at nucleotides 954-956. A putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 2A, and the start and stop codons are in bold letters. The representative ORF encodes a 311 amino acid polypeptide (SEQ ID NO. : 2) with a predicted molecular weight of 34796.5 daltons (Da). PSORT analysis of a NOVl polypeptide predicts a plasma membrane protein with a certainty of 0.6400 and an endoplasmic reticulum membrane protein with a certainty of 0.6850. SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 55 and 56 in SEQ ID NO.: 2.
TABLE 2A. CTTATGCATTCACAAGCAGGATGTTCCTTCCCAATAACACCCAGTTTCACCCC
TCCTCCTTCCTGTTGCTGGGGATCCCAGGGCTAGAAACACTTCACATCTGGATCGG CTTTCCCTTTTGTGCTGTGTACATAATTGCACTCATAGGGCGCTTCACTATTCTACT TGTGATCAAGACTGACAGCAGCCTATACCAGCCCATGTTCTACTTCCTGGCCATGT TGGCCACCATTGACTTGGGCCTTTCAACAGCTACCATCCCTAAGATGCTTGGGATC TTCTGGTTTAGCCTCAGGGAGATTATCTGTGATGCCTGCCTCATCCAGATGTTTTTC ATCCACAACTTTACTGGCATGGAGTCAGCAGCCCTCGTGGGAATGGCTTATGACC ACTTTGTGGCCATCTGCAACCCGCTACGATATAGCATCATCCTCACCAAAAAGGCT GTTTCTGTGATTGGTCTTGGTGTGTTAGTGAGGTCATTTATGTCTGTTATTCCATTT GTTTTTCTCATTTTGCGGTTGCCCTTCTGTGGGGATCATGTCATTCCCCACACCAAC TGTGAGCAC ATGGGTCTTGCTC ATCTGTCTTGTTCCAGTATC AAGATC AATATAAT CTATGGCTTGGGTGCTATTTCAATCCTAGTATTCGACATCATAGCCATTGCCCTTTC TTATGTGCAAATACTTCACGCTGTTTTCCATCTTCCTTCCTGTAAAGCCCGACTCAA GTCCCTCAGCACATGTGGTTCACATGTGTGTGTAATCCTTGCCTTCTATACACCAG CCCTCTTTTCCTTTGTGACTCATCGCTTTGGCCAAAATGTGCCCCGCTATATCCATA TACTCCTAGCCAATCTCTATGTTGTGGTGCCACCAATGCTCAATCCTGTCATATAT GGAGTCAGAACCAAGCAGATCTATGTCTGTGTGAAGAATATATTCTTACAAAAAT 5 AAGAAATTGAAAAGAAATCGCATC (SEQ ID NO. : 1)
MFLPNNTQFPIPSSFLLLGIPGLETLMWIGFPFCAVYJIALIGRFTILLVIKTDSSLYQP MFYFLAMLATROLGLSTATIPHVILGIFWFSLREIICDACLIQMFFJ-HNFTGMESAALVG MAYDHFVAICNPLRYSΠLTKKAVSVIGLGVLVRSFMSVJTFVFLΓLRLPFCGDHVΓPHT l o NCEHMGLAHLSCSSIKIMΓYGLGAISILVFDΠAIALSYVQILHAVFHLPSCKARLKSLST
CGSHVCVILAFYTPALFSFVTIffiFGQNWRYffllLLA-^YVVVPPMLNPVrYGVRTKQI YVCVKNIFLQK (SEQ ID NO.: 2)
A NOVl nucleic acid has homology (67% identity) to a Mus musculus GPCR mRNA 15 (MUSI; GENBANK-ID: AF121979), as is shown in Table 2B. A NOVl polypeptide also has homology (63% identity, 80% similarity) with the 318 amino acid residue protein from Mus musculus (MUS2; SPTREMBL-ACC: Q9WU93), as is shown in Table 2C.
TABLE 2B. 0 64 ACATACAGTCATAAAGATCATGGACTTATGCATTCACAAG-CAGGATGTTCCTTCCCAAT 122
I I I I I I I I I I I 1 1 1 I I I I I I I I I I I I I 1 I I I
41 AGATTCCTTCA-AATGAATATGTCCATCAG-AGGCTCCTGACAACATGTCACCAGGCAAC 98
123 AACACCCAGTTTCACCCCTCCTCCTTCCTGTTGCTGGGGATCCCAGGGCTAGAAACACTT 182
25 I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I II II I I II
99 AGCTCATGGATTCATCCTTCTTCCTTCCTGCTCTTGGGAATCCCAGGACTGGAAG-AGTT 157 183 -CACATCTGGATCGGCTTTCCCTTTTGTGCTGTGTACATAATTGCACTCATAGGGCGCTT 241
M I II II I I I I I I I I II I I 1 I I I I I I I I I I I I Ml I I
30 158 GCAGTTCTGGCTTGGTTTGCCATTTGGAACAGTCTATCTTATTGCTGTCCTAGGGAATGT 217 242 CACTATTCTACTTGTGATCAAGACT-GA-CAGCAGCCTATACCAGCCCATGTTCTACTTC 299
II I I I II I I II III I I I I I I I I I I I I I I I I I I I I II III I I I I I
218 CATCATTCTCTTTGTAATCTAT-CTAGAGCA-CAGCCTTCACCAACCTATGTTCTACTTA 275
35
300 CTGGCCATGTTGGCCACCATTGACTTGGGCCTTTCAACAGCTACCATCCCTAAGATG-CT 358 I I II II II I IN I I Mill I I || II I II II II I II I II M
276 CTGGCCATACTGGCTGTTACTGACTTGGGTCTGTCTACAGCAACTGTTCCCA-GAGCACT 334
40 359 TGGGATCTTCTGGTTTAGCCTCAGGGAGATTATCTGTGATGCCTGCCTCATC-CAGATGT 417
I I I I I I I I I I I I I I I I I M i l l M l M M I I I I I I I I I
335 CGGTATATTCTGGTTTGGCTTCCATAAGATTGCCTTTAGGGACTGTGT-AGCTCAAATGT 393 418 TTTTCATCCACAACTTTACTGGCATGGAGTCAG-CAGCCCTCGTGGGAATGGCTTATGAC 476
45 I I I I I II II 1 I I II I MM I I II II I I II I II I II I III
394 TTTTCATACATCTGTTTACAGGCATCGAAACATTCATGCTT-GTAGCTATGGCCTTTGAT 452 477 CACTTTGTGGCCATCTGCAACCCGCTACGATATAGCATCATCCTCACCAAAAAGGCTGTT 536
I II I I I II II I I II I I I II I I I I II I I I II I I I I I I I I I I I I
50 453 CGCTACATTGCCATCTGTAACCCTCTCCGATATAACACTATCCTCACCAACAGAACAATC 512 537 TCTGTGATTGGTCTTGGTGTGTTAGTGAGGTCATTT-ATGTCTGTTATTCCATTTGTTTT 595
I I II II I I II II I I I II I II I I II I I II I II I I I
513 TGCATTATTGTTGGAGTTGGACTATTTAAAA-ATTTCATTTTGGTTTTTCCACTTATATT 571
55 596 TCTCATTTTGCGGTTGCCCTTCTGTGGGGATCATGTCATTCCCCACACCAACTGTGAGCA 655
1 I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M l I I I I I I 572 TCTCATTCTAAGGCTTTCATTCTGTGGACACAATATCATACCACACACATACTGTGAGCA 631
656 CATGGGTCTTGCTCATCTGTCTTGTTCCAGTATCAAGATCAATATAATCTATGGCTTGGG 715
II II II II II II II I I M II I II II II I I II I I I I II I II
632 CATGGGCATTGCTCGACTGGCATGCGTCAGCATCAAGGTTAATGTATTATTTGGATTAA- 690 716 TGCT-ATTTCAATCCTAGTATTCGACATCATAGCCATTGCCCTTTCTTATGTGCAAATAC 774
I I I I I I I II II I I II I I I 111 II I I II II I I II I I
691 TACTCATATCTATGATACTTCTGGATGTTGTTTTGAGTGCTCTGTCCTATGCGAAAATTC 750
775 TTCACGCTGTTTTCCATCTTCCTTCCTGTAAAGCCTGACTCAAGTCCCTCAGCACATGTG 834
II 11 II III I I I II II Mill Mill I I I 1 II I III
751 TTCATGCTGTATTTAAACTCCCATCCTGGGAAGCCAGACTCAAAGCTCTTAATACCTGTG 810 835 GTTCACATGTGTGTGTAATCCTTGCCTTCTATACACCAGCCCTCTTTTCCTTTGTGACTC 894
I I II I II I I II II II III I II II I I II I I II II I II Mill I II Ml
811 GTTCCCATGTGTGTGTGATCTTGGCTTTGTTCACTCCAGCCTTTTTCTCCTTCTTGACTC 870 895 ATCGCTTTGGCCAAAATGTGCCCCGCTATATCCATATACTCCTAGCCAATCTCTATGTTG 954
II I I Mill M III I II II II II II II II II I II II II I Mill
871 ATCGATTTGGACACAATATTCCACGATATATCCACATCCTCCTTGCTAACTTATATGTGA 930 955 TGGTGCCACCAATGCTCAATCCTGTCATATATGGAGTCAGAACCAAGCAGATCTATG-TC I I II I II I I II I I II II I I I II I I I I III I I Mil I I I I
931 TCATTCCCCNGGCTCTTAACCCTATTATTTATGGGGTGAGAACCAAACAGATACAAGATC 990 1014 TGTGTGAAGAATATATTCTTACAA 1037 (SEQ ID NO.: 25)
991 -GTGCGGTGΆCAATATTGTG-CAA 1012 (SEQ ID NO.: 26)
TABLE 2C.
NOVl 5 NNTQFHPSSFLLLGIPGLETLHIWIGFPFCAVYIIA IGRFTILLVIKTDSSLYQPMFYF 64
I++ I I I I I I I I I I II I I I l + l II I l + l I++I I I I I + 11 + 11111 MUS2 5 NSS IHPSSFLLLGIPGLEELQF GLPFGTVYLIAVLGNVIILFVIYLEHSLHQPMFYL 64 NOVl 65 LAMLATIDLGLSTATIPKMLGIFWFSLREIICDACLIQMFFIHNFTGMΞSAALVGMAYDH 124
I l + l I lllllll 1 + 1+ I I II II +1 1+ II I I I I I I 1 + 1+ II I l + l MUS2 65 LAILAVTDLGLSTATVPRALGIF FGFHKIAFRDCVAQMFFIHLFTGIETFMLVAMAFDR 124 NOVl 125 FVAICNPLRYSIILTKKAVSVI-GLGVLVRSFMSVIPFVFLILRLPFCGDHVIPHTNCEH 183
++I I I I II I 1+ III + + +1 l + l 1 ++I+ I I +1 I I 1 I I III ++I I I I III MUS2 125 YIAICNPLRYNTILTNRTICIIVGVG-LFKNFILVFPLIFLILR SFCGHNIIPHTYCEH 183 NOVl 184 MGLAHLSCSSIKINIIYGLGAISILVFDIIAIALSYVQILHAVFHLPSCKA*LKSLSTCG 243 i i + i i + i H I + I +++I I n +++ ι ++ m i + 1 1 1 1 1 1 1 1 1 + 1 1 1 + 1 + 1 1 1 MUS2 184 MGIARLACVSIKVNVLFGLILISMILLDVVLSALSYAKILHAVFKLPS EARLKALNTCG 243 NOVl 244 SHVCVILAFYTPALFSFVTHRFGQNVPRYIHILLANLYVVVPPMLNPVIYGVRTKQI 300
II I II I I II + II I 1 I I + 1 I I I I I + II I M II I II I I I ++ 1 I II + II I I II I II MUS2 244 SHVCVILAFFTPAFFSFLTHRFGHNIPRYIHILLANLYVIIPXALNPIIYGVRTKQI 300 NOVl (SEQ ID NO. : 27) MUS2 (SEQ ID NO. 28'
Where | indicates identity and + indicates similarity. NOVl expression was analyzed by TaqMan analysis. Results shown in Example 4, Table 14C demonstate that NOVl expression is higher in lung cancer (small cell), colon cancer, ovarian cancer, prostate cancer and uterine cancer as compared to normal tissue.
NOV2
A NOV2 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins. A NOV2 nucleic acid is found on human chromosome 11. A NOV2 nucleic acid and its encoded polypeptide include the sequences shown in Table 3 A. The disclosed nucleic acid (SEQ ID NO.: 3) is 983 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 21-23 and ends with a TAA stop codon at nucleotides 960-962. A putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 3A, and the start and stop codons are in bold letters. The representative ORF encodes a 313 amino acid polypeptide (SEQ ID NO.: 4) with a predicted molecular weight of 35077.8 Da. PSORT analysis of a NOV2 polypeptide predicts a plasma membrane protein with a certainty of 0.600. Using SIGNALP analysis, it is predicted that a NOV2 polypeptide has no signal peptide.
TABLE 3A. CAGAGCCCTATGAATGAATCATGTCCATTATCAACACATCATATGTTGAAATCACCACCTT
CTTCTTGGTTGGGATGCCAGGGCTAGAATATGCACACATCTGGATCTCTATCCCCATCTGCAG CATGTATCTTATTGCTATTCTAGGAAATGGCACCATTCTTTTTATCATCAAGACAGAGCCCTC CTTGCATGGGCCCATGTACTATTTTCTTTCCATGTTGGCTATGTCAGACTTGGGTTTGTCTTT ATCATCTCTGCCCACTGTGTTAAGCATCTTCCTGTTCAATGCCCCTGAAACTTCTTCTAGTGC CTGCTTTGCCCAGGAATTCTTCATTCATGGATTCTCAGTACTGGAGTCCTCAGTCCTCCTGAT CATGTCATTTGATAGATTCCTAGCCATCCACAATCCTCTGAGATACACCTCAATCCTGACAAC TGTCAGAGTTGCCCAAATAGGGATAGTATTCTCCTTTAAGAGCATGCTCCTGGTTCTTCCCTT CCCTTTCACTTTAAGAAGCTTGAGATATTGCAAGAAAAACCAATTATCCCATTCCTACTGTCT CCACCAGGATGTCATGAAGTTGGCCTGTTCTGACAACAGAATTGATGTTATCTATGGCTTTTT TGGAGCACTCTGCCTTATGGTAGACTTTATTCTCATTGCTGTGTCTTACACCCTGATCCTCAA GACTGTACCGGGAATTGCATCCAAAAAGGAGGAGCTTAAGGCTCTCAATACTTGTGTTTCACA CATCTGTGCAGTGATCATCTTCTACCTGCCCATCATCAACCTGGCCGTTGTCCACCGCTTTGC CGGGCATGTCTCTCCCCTCATTAATGTTCTCATGGCAAATGTTCTCCTACTTGTACCTCCGCT GATGAAACCAATTGTT ATTGTG AAAAACTAAACAGATTAGAGTGAGAGTTGTAGCAAAATT GTGTCAATGGAAGATTTAACAGTCATATGTGACAGAAAAC (SEQ IDNO.: 3)
MSIINTSYVEITTFFLVGMPGLEYAHIWISIPICSMYLIAILGNGTILFIIKTEPSLHGPMYY FLSMLAMSDLGLSLSSLPTVLSIFLFNAPETSSSACFAQEFFIHGFSVLESSVLLIMSFDRFL AIHNPLRYTSILTTVRVAQIGIVFSFKSMLLVLPFPFTLRSLRYCKKNQLSHSYCLHQDVMKL ACSDNRIDVIYGFFGALCLMVDFILIAVSYTLILKTVPGIASKKEELKALNTCVSHICAVIIF YLPIINLAVVHRFAGHVSPLINVLMANVLLLVPPLMKPIVYCVKTKQIRVRVVAKLCQWKI (SEQ ID NO.: 4) A NOV2 nucleic acid has homology (63% identity) with a Gallus gallus GPCR mRNA (GALl; GENBANK-ID: CHKHBBRE/acc:L17432), as is shown in Table 3B. A NOV2 polypeptide has homology (51% identity, 70% similarity) with a 319 amino acid residue olfactory receptor-like protein from Gallus gallus (GALl ; SPTREMBL-ACC: Q9YH55), as shown in Table 3C. A NOV2 polypeptide also has homology (46% identity, 67% similarity) to a HOR 5'BETA3 polypeptide from Homo sapiens (HORl; SPTREMBL-ACC: Q9Y5P1), as is shown in Table 3D.
TABLE 3B.
NOV2: 201 TCACCACCTTCTTCTTGGTTGGGATGCCAGGGCTAGAATATGCACACATCTGGATCTCTA 260
. II I I I II I I Ml M I II I II I I I III MU M GALl : 29039 TCAGC-CTTTCCTGCTGGCAGGCCTTCCCGGGATGGCTCAGTTTCACCACTGGGTGTTCC
29097
NOV2: 261 TCCCCATCTGCAGCATGTATCTTATTGCTATTCTAGGAAATGGCACCATTCTTTTTATCA 320
MM I I II II II II I MM I II II II III I III II II I I GALl : 29098 TCCCTTTTGGTCTCATGTATCTGGTTGCAGTGCTGGGAAATGGTACCATCCTGCTGGTTG 29157
NOV2: 321 TCAAGACAG-AGC-CCTCCTTGCATGGGCCCATGTACTATTTTCTTTCCATGTTGGCTAT 378
I I I I I I I II II II II II I II I I I II III I I II II I 1 II I 1
GALl: 29158 TGA-GAGTGCATCGCCAGCTC-CACCAGCCCATGTACTATTTCCTTTTGATGCTGGCCAC 29215
NOV2: 379 GTCAGACTTGGGTTTGTCTTTATCATCTCTGCCCACTGTGTTAAGCATCTTCCTGTTCAA 438
I 1 II I I II I ll ll l ll I II I II 111 I I I ll lllll III
GALl : ' 29216 CACAGACCTGGGCCTGACTCTGTCCACCCTGCCCACCGTTCTGCGCGTCTTCTGGTTGGG 29275
NOV2 : 439 TGCCCCTGAAA-CTTCTTCTAGTGCCTGCTTTGCCCAGGAATTCTTCATTCATGGATTCT 497
I I I I I I I I I I M l l l l l l l l I I I I I I I I I I I I I I I 1 1 1
GALl: 29276 TGCCATGGAAATCAGCTTCCCC-GCCTGCCTCATCCAGATGTTCTGCATCCATGTCTTTT 29334
NOV2: 498 CAGTACTGGAGTCCTCAGTCCTCCTGATCATGTCATTTGATAGATTCCTAGCCATCCACA 557
I I I I M I M I I M M M i l l I I I I I 1 M l I I I I I l I
GALl: 29335 CCTTCATGGAGTCCTCAGTGCTCCTGGCCATGGCCTTTGATCGCTATGTGGCCATCTGCT 29394
NOV2: 558 ATCCTCTGAGATACACCTCAATCCTGACAACTGTCAGAGTTGCCCAAATAGGGATAGTAT 617
I I II I I I II I I I II MM I II I I I II I II II I I I I I I I I
GALl: 29395 GCCCGCTGAGGTACTCCTCCATCCTCACTGGTGCCAGGGTTGCACAGATTGGACTGGGGA 29454
NOV2: 618 TCTCCTTTAAGA-GCATGCTCCTGGTTCTTCCCTTCC-CTTTCACTTTAAGAAGCTTGAG 675
M II 11 I I I II II II I I 1! II I I I I I II I 1
GALl: 29455 TCATCTGCC-GATGCACACTGTCACTTCTCCCATTAATCTGCCTCCTGACGTGGCT-GCC 29512
NOV2: 676 ATATTGCAAGAAAAACCAATTATCCCATTCCTACTGTCTCCACCAGGATGTCATGAAGT- 734
I Mil l I I I I I II II II II II II II I II II II I II I I GALl : 29513 TTTCTGCAGGTCACATGTGCTTTCTCACCCCTACTGTCTGCACCAGGATATTAT-ACGAC 29571
NOV2: 735 TGGCCTGTTCTGACAACAGAATTGATGTTATCTATGGCTTT-T-TTGGAGCACTCTGCCT 792
M i l l I I I M I I I I I I I I I I I I I I I I I I I GALl : 29572 TAGCGTGCACAGATGCCACCCTGAACAGCCTGTATGGATTAATCTTGGTGTTGGTTGCA- 29630
NOV2: 793 TATGGTAGACTTTATTCTCATTGCTGTGTCTTACACCCTGATCCTCAAGACTGTACCGGG 852
II MM II MM I II II I I II I I I I I I I I II II I I I I I II I I II
GALl : 29631 -ATCCTGGACTTTGTTCTCATTGCGTTGTCCTACATCATGATCTTTAGGACTGTGCTAGG 29689
N0V2: 853 AATTGCATCCAAAAAGGAGGAGCTTAAGGCTCTCAATACTTGTGTTTCACACATCTGTGC 912
II I II I II I III I I II II II II II II II II I I II III I MM II
GALl: 29690 CATCACCTCCAAAGAGGAGCAGACCAAAGCCCTAAACACTTGTGTCTCTCACTTCTGTGC 29749
NOV2: 913 AGTGATCATCTTCTACCTGCCCATCATCAAC-CTGGCCGTTGTCCACCGCTTTGCCGGGC 971 II IN I || II M I M II I I II I I I II I II I I I I I I II
GALl : 29750 CGTCCTCATCTTCTACAT-CCCTTTGGCTGGGCTGTCCATTATACACCGGTATGGAAGGA 29808
NOV2 : 972 ATGTCTCTCCCCTCATTAATGTTCTCATGGCAAATGTTCT-CCTACTTGTACCTCCGCTG 1030 lll l l ll I I II I I 111 I II I I I I II I I I II II II II I I 1
GALl : 29809 ATGCCCCACCTATTAGCCATGCTGTCATGGCCAATGT-CTACCTCTTTGTCCCACCTATA 29867 NOV2: 1031 ATGAAACCAATTGTTTATTGTGTAAAAACTAA 1062 (SEQ ID NO.: 31)
MM II I I 1 1 I I I I II I II GALl: 29868 CTGAACCCAGTCCTCTACAGCATGAAAAGCAA 29899 (SEQ ID NO.: 32)
TABLE 3C.
NOV2 14 FFLVGMPGLEYAHI ISIPICSMYLIAILGNGTILFIIKTEPSLHGPMYYFLSMLAMSDL 73
I 1 1 + 11+ 1 1+ +1 I M + l + M II I II +++ I I I II II I I II +1 I OLR1 12 FLLAGLPGMAQFHH VFLPFGLMYLVAVLGNGTILLVVRVHRQLHQPMYYFLLMLATTDL 71 NOV2 74 GLSLSSLPTVLSIFLFNAPETSSSACFAQEFFIHGFSVLESSVLLIMSFDRFLAIHNPLR 133
M + M + IMM +I 1 1 1 II I I 1 I I I +1 I I I I I l + l I I++I I 111 OLR1 72 GLTLSTLPTVLRVF LG7AMEISFPACLIQMFCIHVFSFMESSVLLAMAFDRYVAICCPLR 131 NOV2 134 YTSILTTVRVAQIGIVFSFKSMLLVLPFPFTLRSLRYCKKNQLSHSYCLHQDVMKLACSD 193 l + l I I I I I I I I 1+ + I +11 I I +1+ + I I I I I I I I I+++I I l + l OLR1 132 YSSILTGARVAQIGLGIICRCTLSLLPLICLLT LPFCRSHVLSHPYCLHQDIIRLACTD 191 NOV2 194 NRIDVIYGFFGALCLMVDFILIAVSYTLILKTVPGIASKKEELKALNTCVSHICAVIIFY 253
++ +II I ++I l + l I l + l I +1 +1 I II I 1 + 1+ I I I I I I I I I I I l + l I I OLR1 192 ATLNSLYGLILVLVAILDFVLIALSYIMIFRTVLGITSKEEQTKALNTCVSHFCAVLIFY 251 NOV2 254 LPIINLAVVHRFAGHVSPLINVLMANVLLLVPPLMKPIVYCVKTKQIRVRVVAKLCQ 310
+ 1+ I+++M+ + 1+ + +11 II I I I I++ I++I +1 + 1 I ++ III OLR1 252 IPLAGLSIIHRYGRNAPPISHAVMANVYLFVPPILNPVLYSMKSKAICKGLLRLLCQ 308 NOV2 (SEQ ID NO. : 33) OLR1 (SEQ ID NO. : 34)
Where | indicates identity and + indicates similarity.
TABLE 3D.
NOV2: 14 FFLVGMPGLEYAHI ISIPICSMYLIAILGNGTILFIIKTEPSLHGPMYYFLSMLAMSDL 73
I I I MM II II I II ++I+ +11 II +I++I I + III I I I I I l + l I I +11 HORl: 10 FLLTGFPGLEAAHHWISIPFFAVYVCILLGNGMLLYLIKHDHSLHEPMYYFLTMLAGTDL 69
NOV2: 74 GLSLSSLPTVLSIFLFNAPETSSSACFAQEFFIHGFSVLESSVLLIMSFDRFLAIHNPLR 133
++I+++I 11+ I I I I I I I I +1 II I l + l I I I I++ 1 I l + l I I I I I
HORl: 70 MVTLTTMPTVMGIL VNHREISSVGCFLQAYFIHSLSVVESGSLLAMAYDRFIAIRNPLR 129 NOV2: 134 YTSILTTVRVAQIGIVFSFKSMLLVLPFPFTLRSLRYCKKNQLSHSYCLHQDVMKLACSD 193 I I I I I I + 1 + + + + 1 1 I I I I I + ++ ++ M M ++ I + M l + l
HORl 130 YASIFTNTRVIALGVGVFLRGFVSILPVILRLFSFSYCKSHVITRAFCLHQEIMRLACAD 189 NOV2 194 NRIDVIYG-FFGALCLMVDFILIAVSYTLILKTVPGIASKKEELKALNTCVSHICAVIIF 253
+ + I + I + + I ++ 1 I I M i l l M M + 1 I I 1 I 1 l + l I I I + 1 1
HORl 190 ITFNRLYPVILISLTIFLDSLIILFSYILILNTVIGIASGEERAKALNTCISHISCVLIF 249 NOV2 254 YLPIINLAVVHRFAGHVSPLINVLMANVLLLVPPLMKPIVYCVKTKQIRVRVVAKL 309
1+ ++ I ++|| +| +++++I+ + l MM I++I +11 II 1+ ++ I HORl 250 YVTVMGLTFIYRFGKNVPEVVHIIMSYIYFLFPPLMNPVIYSIKTKQIQYGIIRLL 305 NOV2 (SEQ ID NO. : 35) OLR1 (SEQ ID NO. : 36) Where | indicates identity and + indicates similarity.
The DNA and protein sequences for the novel single nucleotide polymorphic (SNP) variants of the olfactory receptor-like gene of NOV2 are in Table 3E. The method of identifying and confirming novel SNPs is described in Example 3. Table 3E. SNPs
1(a). Variant 1 of NOVl nucleotide sequence.
T/G at position 81.
1(b). Nucleotide sequence of variant 1.
1
TACTGTTAAATCTTCCATTGACACAATTTTGCTACAACTCTCACTCTAATCTGTTTAGTTTTTACACAATAAACAAT
TGG
81 CTTCATCAGCGGAGGTACAAGTAGGAGAACATTTGCCATGAGAACATTAATGAGGGGAGAGACATGCCGGGCAAAGC
GGT
161
GGACAACGGCCAGGTTGATGATGGGCAGGTAGAAGATGATCACTGCACAGATGTGTGAAACACAAGTATTGAGAGCC
TTA 241 GCTGCTCCTTTTTGGATGCAATTCCCGGTACAGTCTTGAGGATCAGGGTGTAΆGACACAGCAATGAGAΆTAAAGTC
TAC 321 CAT- AGGCAGAGTGCTCCA-Y-AAAGCCATAGATAACATCAATTCTGTTGTCAG- ACAGGCCAFT.CTTCATGACATCCT GGT 401
GGAGACAGTAGGAATGGGATAATTGGTTTTTCTTGCAATATCTCAZ-GCTTCTTAAAGTGAAAGGGAAGGGAAGAA.CC AGG 481 AGCATGCTCTTAAAGGAGAATACTATCCCTATTTGGGCAACTCTGACAGTTGTCAGGATTGAGGTGTATCTCAGAGG
ATT 561
GTGGATGGCTAGGAATCTATCAAATGACATGATCAGGAGGACTGAGGACTCCAGTACTGAGAATCCATGAATGAAGA
ATT 641 CCTGGGCAAAGCAGGCATTGGATGAAGTTTCAGGAGCATTGAACAGGAAGATGCTTAACACAGTGGGCAGAGATGAT
AAA 721 GACAAACCCAAGTCTGACATAGCCAACATGGAAAGAAAATAGTACATGGGCCCATGCAAGGAGGGCTCTGTCTTGAT GAT 801
AAAAAGAATGGTGCCATTTCCTAGAATAGCAATAAGATACATGCTGCAGATGGGGATAGAGATCCAGATGTGTGCAT ATT
881 CTAGCCCTGGCATCCCAACCAAGAAGAAGGTGGTGATTTCAACATATGATGTGTTGATAATGGACATGATTA (SEQ ID NO. : 29) 1(c). Protein Sequence of variant 1.
1
MSIINTSYVEITTFFLVGMPGLEYAHIWISIPICSMYLIAILGNGTILFIIKTEPSLHGPMYYFLSMLAMSDLGLSL SSL 81
PTVLSIFLFNAPETSSNACFAQEFFIHGFSVLESSVLLIMSFDRFLAIHNPLRYTSILTTVRVAQIGIVFSFKSMLL VLP 161
FPFTLRSLRYCKKNQLSHSYCLHQDVMKLACSDNRIDVIYGFFGALCLMVDFILIAVSYTLILKTVPGIASKKEQLK ALN
241 TCVSHICAVIIFYLPIINLAVVHRFARHVSPLINVLMANV LLVPPLMKPIVYCVKTKQIRVRWAKLCQ KI (SEQ ID NO. : 30)
1(d). Alteration effect of variant 1 on amino acid residue None
2(a). Variant 2 ofNOV2 nucleotide sequence.
C/G at position 149.
2(b). Nucleotide sequence of variant 2.
1
TACTGTTAAATCTTCCATTGACACAATTTTGCTACAACTCTCACTCTAATCTGTTTAGTTTTTACACAATAAACAAT
TGG
81 TTTCATCAGCGGAGGTACAAGTAGGAGAACATTTGCCATGAGAACATTAATGAGGGGAGAGACATGCCCGGCAAAGC
GGT
161
GGACAACGGCCAGGTTGATGATGGGCAGGTAGAAGATGATCACTGCACAGATGTGTGAAACACAAGTATTGAGAGCC TTA 241
AGCTGCTCCTTTTTGGATGCAATTCCCGGTACAGTCTTGAGGATCAGGGTGTAAGACACAGCAATGAGAATAAAGTC TAG 321
CATAAGGCAGAGTGCTCCAAAAAAGCCATAGATAACATCAATTCTGTTGTCAGAACAGGCCAACTTCATGACATCCT GGT 401
GGAGACAGTAGGAATGGGATAATTGGTTTTTCTTGCAATATCTCAAGCTTCTTAAAGTGAAAGGGAAGGGAAGAACC AGG 481 AGCATGCTCTTAAAGGAGAATACTATCCCTATTTGGGCAACTCTGACAGTTGTCAGGATTGAGGTGTATCTCAGAGG
ATT 561
GTGGATGGCTAGGAATCTATCAAATGACATGATCAGGAGGACTGAGGACTCCAGTACTGAGAATCCATGAATGAAGA ATT 641
CCTGGGCAAAGCAGGCATTGGATGAAGTTTCAGGAGCATTGAACAGGAAGATGCTTAACACAGTGGGCAGAGATGAT AAA 721
GACAAACCCAAGTCTGACATAGCCAACATGGAAAGAAAATAGTACATGGGCCCATGCAAGGAGGGCTCTGTCTTGAT GAT 801
AAAAAGAATGGTGCCATTTCCTAGAATAGCAATAAGATACATGCTGCAGATGGGGATAGAGATCCAGATGTGTGCAT ATT
881 CTAGCCCTGGCATCCCAACCAAGAAGAAGGTGGTGATTTCAACATATGATGTGTTGATAATGGACATGATTA (SEQ ID NO. : 37)
2(c). Protein Sequence of variant 2.
1
MSIINTSYVEITTFF VGMPGLEYAHI ISIPICSMYLIAILGNGTILFIIKTEPSLHGPMYYFLSMLAMSDLGLSL ssL
81
PTVLSIFLFNAPETSSNACFAQEFFIHGFSVLESSVLLIMSFDRF AIHNPLRYTSILTTVRVAQIGIVFSFKSMLL VLP 161 FPFTLRSLRYCKKNQLSHSYCLHQDVMKLACSDNRIDVIYGFFGALCL VDFILIAVSYTLILKTVPGIASKKEQLK
ALN
241 TCVSHICAVIIFYLPIINLAWHRFAGHVSPLINVLMANVLLLVPPLMKPIVYCVKTKQIRVRVVAKLCQWKI
( SEQ ID NO . : 38 ) 2(d). Alteration effect of variant 2 on amino acid residue
Arg to Gly at position 267.
3(a). Variant 3 ofNOVl.
G/C at postion 245.
3(b). Nucleotide sequence of variant 3.
1 TACTGTTAAATCTTCCATTGACACAATTTTGCTACAACTCTCACTCTAATCTGTTTAGTTTTTACACAATAAACAAT
TGG 81
TTTCATCAGCGGAGGTACAAGTAGGAGAACATTTGCCATGAGAACATTAATGAGGGGAGAGACATGCCGGGCAAAGC GGT 161
GGACAACGGCCAGGTTGATGATGGGCAGGTAGAAGATGATCACTGCACAGATGTGTGAAACACAAGTATTGAGAGCC TTA 241
AGCTCCTCCTTTTTGGATGCAATTCCCGGTACAGTCTTGAGGATCAGGGTGTAAGACACAGCAATGAGAATAAAGTC TAG 321
CATAAGGCAGAGTGCTCCAAAAAAGCCATAGATAACATCAATTCTGTTGTCAGAACAGGCCAACTTCATGACATCCT GGT 401 GGAGACAGTAGGAATGGGATAATTGGTTTTTCTTGCAATATCTCAAGCTTCTTAAAGTGAAAGGGAAGGGAAGAACC
AGG 481
AGCATGCTCTTAAAGGAGAATACTATCCCTATTTGGGCAACTCTGACAGTTGTCAGGATTGAGGTGTATCTCAGAGG ATT 561
GTGGATGGCTAGGAATCTATCAAATGACATGATCAGGAGGACTGAGGACTCCAGTACTGAGAATCCATGAATGAAGA ATT 641
CCTGGGCAAAGCAGGCATTGGATGAAGTTTCAGGAGCATTGAACAGGAAGATGCTTAACACAGTGGGCAGAGATGAT AAA 721
GACAAACCCAAGTCTGACATAGCCAACATGGAAAGAAAATAGTACATGGGCCCATGCAAGGAGGGCTCTGTCTTGAT GAT 801 AAAAAGAATGGTGCCATTTCCTAGAATAGCAATAAGATACATGCTGCAGATGGGGATAGAGATCCAGATGTGTGCAT
ATT
881 CTAGCCCTGGCATCCCAACCAAGAAGAAGGTGGTGATTTCAACATATGATGTGTTGATAATGGACATGATTA (SEQ ID NO. : 39) 3(c). Protein Sequence of variant 3.
1
MSIINTSYVEITTFFLVGMPGLEYAHI ISIPICSMYLIAILGNGTILFIIKTEPSLHGP YYFLSMLAMSDLG SL
SSL
81 PTVLSIFLFNAPETSSNACFAQEFFIHGFSVLESSVLLI SFDRFLAIHNPLRYTSILTTVRVAQIGIVFSFKSMLL
VLP
161
FPFTLRSLRYCKKNQLSHSYCLHQDVMKLACSDNRIDVIYGFFGALCLMVDFILIAVSYTLILKTVPGIASKKEELK ALN 241 TCVSHICAVIIFYLPIINLAVVHRFARHVSPLINVL ANVLLLVPPLM PIVYCVKTKQIRVRVVAKLCQWKI (SEQ ID NO. : 40)
3(d). Alteration effect of variant 3.
Gin to Glu at position 235. NOV2 expression was analyzed by TaqMan analysis. Results shown in Example 4, Table 15B demonstate that NOV2 expression is higher in lung cancer (small cell) as compared to normal tissue.
NOV3
A NOV3 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins. A NOV3 nucleic acid is found on human chromosome 11. A NOV3 nucleic acid and its encoded polypeptide includes the sequences shown in Table 4A. The disclosed nucleic acid (SEQ LD NO.: 5) is 980 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 25-27 and ends with a TAA stop codon at nucleotides 964-966. A putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 4A, and the start and stop codons are in bold letters. The representative ORF encodes a 313 amino acid polypeptide (SEQ ID NO.: 6) with a predicted molecular weight of 35255.9 Da. PSORT analysis of aNOV3 polypeptide predicts aplasma membrane protein with a certainty of 0.6000. Using SIGNALP analysis, it is predicted that a NOV3 polypeptide has no signal peptide.
TABLE 4A. ATAGCAGAGCCCTATGAATGAATCATGTCCATTATCAACACATCATATGTTGAAA TCACCACCTTCTTCTTGGTTGGGATGCCAGGGCTAGAATATGCACACATCTGGATC TCTATCCCCATCTGCAGCATGTATCTTATTGCTATTCTAGGAAATGGCACCATTCTT TTTATCATCAAGACAGAGCCCTCCTTGCATGAGCCCATGTACTATTTTCTTTCCAT GTTGGCTATGTCAGACTTGGGTTTGTCTTTATCATCTCTGCCCACTGTGTTAAGCAT CTTCCTGTTCAATGCTCCTGAAATTTCATCCAATGCCTGCTTTGCCCAGGAATTCTT CATTCATGGATTCTCAGTACTGGAGTCCTCAGTCCTCCTGATCATGTCATTTGATA GATTCCTAGCCATCCACAACCCTCTGAGATACACCTCAATCCTGACAACTGTCAGA GTTGCCCAAATAGGGATAGTATTCTCCTTTAAGAGCATGCTCCTGGTTCTTCCCTT CCCTTTCACTTTAAGAAACTTGAGATATTGCAAGAAAAACCAATTATCCCATTCCT ACTGTCTCCACCAGGATGTCATGAAGTTGGCCTGTTCTGACAACAGAATTGATGTT ATCTATGGCTTTTTTGGAGCACTCTGCCTTATGGTAGACTTTATTCTCATTGCTGTG TCTTACACCCTGATCCTCAAGACTGTACTGGGAATTGCATCCAAAAAGGAGCAGC TTAAGGCTCTCAATACTTGTGTTTCACACATCTGTGCAGTGATCATCTTCTACCTGC CCATCATCAACCTGGCCGTTGTCCACCGCTTTGCCCGGCATGTCTCTCCCCTCATT AATGTTCTCATGGCAAATGTTCTCCTACTTGTACCTCCACTGACGAACCCAATTGT TTATTGTGTAAAAACTAAACAGATTAGAGTGAGAGTTGTAGCAAAATTGTGTCAA CGGAAGATTTAACAGTCATATGTGAC (SEQ LD NO.: 5) MSIL TSYVEITTFFLVGMPGLEYAmWISMCSMYLIAILGNGTILFIIKTEPSLHEPMYY FLSMLAMSDLGLSLSSLPTVLSLFLFNAPEISSNACFAQEFFIHGFSVLESSVLLLMSFDR FLA-HNPLRYTSILTTVRVAQIGIWSFKSMLLVLPFPFTLR .RYCKKNQLSHSYCLHQ DVMKLACSDNRTOV-YGFFGALCLMVDFILIAVSYTLILKTVLGIASKKEQLKALNTCV SfflCAVI-FYLPIINLAVVHRFAR-EWSPLINVLMA-tWLLLVPPLT^ WAKLCQRKI (SEQ LD NO.: 6)
A NOV3 nucleic acid has homology (61% identity) to a Homo sapiens GPCR mRNA (GPCR; patn: T59274), as is shown in Table 4B. A NOV3 polypeptide has homology (53% identity, 72%> similarity) with a 319 amino acid residue olfactory receptor-like protein from Gallus gallus (GALl; SPTREMBL-ACC: Q9YH55), as is shown in Table 4C. Additional homology (46% identity, 69% similarity) to a HOR 5'BETA3 polypeptide from Homo sapiens (HORl; SPTREMBL-ACC: Q9Y5P1) was also identified, as is shown in Table 4D.
TABLE 4B.
163 TCATGTCCAT-TATCAACACAT-CATATGTTGAAATCACCACCT-TCTTCTTGGTTGGGA 219
Ml II I I MM I I II I 1 11 I II II I I I I II 111 I I
262 TCAGTTCCAGCTATGAGTTCCTGCAACTTCACACATG-CCACCTGTGTGCTTA-TTGGTA 319 220 TGCCAGGGCTAGAATATGCACACATCTGGATCT-CTATCCCCATCTGCAGCATGTATCTT 278
I I 1 II I I I I I I II II 111 II I II II I I I I I MM II I I
320 TCCCAGGATTAGAGAAAGCCCATTTCTGGGTTGGCT-TCCCCCTCCTTTCCATGTATGTA 378 279 ATTGCTAT-TCTAGGAAATGGCACCATTCTTTTTATCATCAAGACAGAGCCCTCCTTGCA 337
I I M I I I II II I III I I M M M I I M M I I I Mill
379 GTGGCAATGTGT-GGAAACTGCATCGTGGTCTTCATCGTAAGGACGGAACGCAGCCTGCA 437 338 TGAGCCCATGTACTATTTTCTTTCCATGTTGGCTATGTCA—GACTTGGGTTTGTCTTTA 395
I I I I I I Ml I II I I I I Ml I I I I I I I I II I II I I II I
438 CGCTCCGATGTACCTCTTTCTCTGCATGCTTGC-A-GCCATTGACCTGGCCTTATCCACA 495 396 TCATCTCTGCCCACTGTGTTAAGCATCTTCCTGTTCAATGCTCCTGAAATTT-CATCCAA 454
II I II II I I I I I I 1 I III II I I II II I III I
496 TCCACCATGCCTAAGATCCTTGCCCTTTTCTGGTTTGATTCCCGAGAGATTAGCATTGAG 555
455 TGCCTGCTTTGCCCAGGAATTCTTCATTCATGGATTCTCAGTACTGGAGTCCTCAGTCCT 514 Mill II II II I I II M II II II I II II II M Ml M MM
556 -GCCTGTCTTACCCAGATGTTCTTTATTCATGCCCTCTCAGCCATTGAATCCACCATCCT 614 515 CCTGATCATGTCATTTGATAGATTCCTAGCCATCCACAACCCTCTGAGATACACCTCAAT 574
Ml II II M II 11 M l I I II I I I I I 1 I 11 1
615 GCTGGCCATGGCCTTTGACCGTTATGTGGCCATCTGCCACCCACTGCGCCATGCTGCAGT 674
575 CCTGA-CAACTGTCAG-AGTTGCCCAAATAGGGATAGTATTCTCCTTTAAGAGCATGCTC 632 ll l lll l MM II III II I I I I I I II I I I II I
675 GCTCAACAA-TA-CAGTAACAGCCCAGATTGGCATCGTGG-CTGTGGTCCGCGGATCCCT 731 633 CTGGTTCTTCCCT-TCCCTTT-CACTTTAAGAAACTTGAGATATTGCAAGAAAAACCAAT 690
II I I I I M I I I I I I I I I II II I I I II I M
732 CTTTTTTTTCCCACTGCCTCTGCTGATCAAGCGGCTGGCCTTCT-GCCACTCCAATGTCC 790 691 TATCCCATTCCTACTGTCTCCACCAGGATGTCATGAAGTTGGCCTGTTCTGACAACAGAA 750
I II II II II I III I II I I I I I I II I I II I I I II II I I II I I I I I I
791 TCTCGCACTCCTATTGTGTCCACCAGGATGTAATGAAGTTGGCCTATGCAGACACTTTGC 850 751 TTGATGTTATCTATGGCTTTTTTGGAGCACT-CTGC-CTTATG-GTAGACTTTATTCTCA 807 II II I II II I II II II I I I I I 1 II II I I I I III
GPCR 851 CCAATGTGGTATATGGTCTTACTGCCATTCTGCTGGTCATGGGCGTGGACGTAATGTTCA 910
NOV3 808 T-TGCTGTGTCTTACACCCTGATCCTCAAGA-CTGTACTGGGAATTGCAT-CCAAAAAGG 864
I I II I I I 1 11 II I I I I I I 1 1 11 I II I I I I I I II I I I
GPCR 911 TCTCCT-TGTCCTATTTTCTGATAAT-ACGAACGGTTCTGC-AACTGCCTTCCAAGTCAG 967
NOV3 865 AGCAGCTTAAGGC-TCTCAATACTTGTGTTTCACACATCTGTGCAGTGATCATCTTCTAC 923
I I I I lllll l l I I I I II II I II I I II I II I II II II I I I I
GPCR 968 AGCGGGCCAAGGCCTTTGGA-ACCTGTGTGTCACACATTGGTGTGGTACTCGCCTTCTAT 1026
NOV3: 924 CTGCCCATCATCAACCTGGCCGTTGTCCACCGCTTTGCCCGGCATGTCT-C-TCCCCTCA III I Ml III I I II II II II II II II II I I I I II II I
GPCR: 1027 GTGCCACTTATTGGCCTCTCAGTTGTACACCGCTTTGGAAA-CA-GCCTTCATCCCATTG 1084
NOV3: 982 TTAATGTTCTCATGGCAAATGTTCT-CCTACTTGTACCTCCACTGACGAACCCAATTGTT 1040
I I I II I II II I I I I I I II II I I I I II I I II I I I I I
GPCR: 1085 TGCGTGTTGTCATGGGTGACAT-CTACCTGCTGCTGCCTCCTGTCATCAATCCCATCATC 1143
NOV3 1041 TATTGTGTAAAAACTAAACAGATTAGAGTGAGAGTTGTAGCAAAATT 1087
I I I I I I II 11 MM II II MM I II I I I I II GPCR 1144 TATGGTGCCAAAACCAAACAGATCAGAACACGGGTGCTGGCTATGTT 1190 NOV3 (SEQ ID NO. 41) GPCR (SEQ ID NO. 42)
TABLE 4C.
NOV3 14 FFLVGMPGLEYAHI ISIPICSMYLIAILGNGTILFIIKTEPSLHEPMYYFLSMLAMSDL 73
I M + M+ M+ +I I I l + l + l I I I I I I +++ M + M MIMM +M GALl 12 FLLAGLPGMAQFHH VFLPFGLMYLVAVLGNGTILLVVRVHRQLHQPMYYFLLMLATTDL 71 NOV3 74 GLSLSSLPTVLSIFLFNAPEISSNACFAQEFFIHGFSVLESSVLLIMSFDRFLAIHNPLR 133
M + M + IMM +I I I II II I I I I I I +1 I I I 1 I l + l I I++I I I II GALl 72 GLTLSTLPTVLRVF LGAMEISFPACLIQMFCIHVFSFMESSVLLAMAFDRYVAICCPLR 131 NOV3 134 YTSILTTVRVAQIGIVFSFKSMLLVLPFPFTLRNLRYCKKNQLSHSYCLHQDVMKLACSD 193 l + l I I I I I I I I 1+ + I +11 I I +1+ + I I I I I I I I I+++I I l + l GALl 132 YSSILTGARVAQIGLGIICRCTLSLLPLICLLT LPFCRSHVLSHPYCLHQDIIRLACTD 191 NOV3 194 NRIDVIYGFFGALCLMVDFILIAVSYTLILKTVLGIASKKEQLKALNTCVSHICAVIIFY 253
++ +II I ++I l + l I l + l I +1 +1 I I I I M + ll I I I I I I I I I IM + MI GALl 192 ATLNSLYGLILVLVAILDFVLIALSYIMIFRTVLGITSKEEQTKALNTCVSHFCAVLIFY 251 NOV3 254 LPIINLAVVHRFARHVSPLINVLMANVLLLVPPLTNPIVYCVKTKQIRVRVVAKLCQRK 312
+ |+ I+++M+ |+ I+ + +IMI I 111+ II++I +1 + 1 I ++ MM GALl 252 IPLAGLSIIHRYGRNAPPISHAVMANVYLFVPPILNPVLYSMKSKAICKGLLRLLCQR 309 NOV3 (SEQ ID NO. 43) GALl (SEQ ID NO. 44)
Where | indicates identity and + indicates similarity.
TABLE 4D.
NOV3 14 FFLVGMPGLEYAHIWISIPICSMYLIAILGNGTILFIIKTEPSLHEPMYYFLSMLAMSDL 73
I I I II I I II Mill ++I+ +1 I I I +I++1I + I I I I I I I I I l + l I I +11 HORl 10 FLLTGFPGLEAAHHWISIPFFAVYVCILLGNGMLLYLIKHDHSLHEPMYYFLTMLAGTDL 69 NOV3 74 GLSLSSLPTVLSIFLFNAPEISSNACFAQEFFIHGFSVLESSVLLIMSFDRFLAIHNPLR 133
++I+++I I 1+ I I I I II I I I +11 I I I + 11 I I I++I M + ll I II I HORl 70 MVTLTTMPTVMGILWVNHREISSVGCFLQAYFIHSLSVVESGSLLAMAYDRFIAIRNPLR 129 NOV3 134 YTSILTTVRVAQIGIVFSFKSMLLVLPFPFTLRNLRYCKKNQLSHSYCLHQDVMKLACSD 193
I I I I I I + 1 + + + + M 1 + I I I + ++ ++ I I I I ++ I + I I l + l HORl 130 YASIFTNTRVIALGVGVFLRGFVSILPVILRLFSFSYC SHVITRAFCLHQEIMRLACAD 189 NOV3 194 NRIDVIYG-FFGALCLMVDFILIAVSYTLILKTVLGIASKKEQLKALNTCVSHICAVIIF 253
+ + I + I + + I ++ I M M M I + M M + I + M M M + I M l + l I HORl 190 ITFNRLYPVILISLTIFLDSL1ILFSYILILNTVIGIASGEERAKALNTCISHISCVLIF 249 NOV3 254 YLPIINLAVVHRFARHVSPLINVLMANVLLLVPPLTNPIVYCVKTKQIRVRVVAKLCQRK 312
1+ ++ 1 ++|| ++I +++++I+ + I 1)1 II++I +IIIII+ ++ I + + HORl 250 YVTVMGLTFIYRFGKNVPEVVHIIMSYIYFLFPPLMNPVIYSIKTKQIQYGIIRLLSKHR 309 NOV3 (SEQ ID NO. 43) GALl (SEQ ID NO. 45)
Where | indicates identity and + indicates similarity.
NOV3 expression was analyzed by TaqMan analysis. The sequence is moderately expressed in many tissues; it is slightly higher in brain regions, especially cerebral cortex, indicating a utility as a marker to identify neuronal derived tissues and cells, as shown in Example 4 at Table 16B.
NOV4 A NOV4 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins. A NOV4 nucleic acid is found on human chromosome 11. A NOV4 nucleic acid and its encoded polypeptide include the sequences shown in Table 5 A. The disclosed nucleic acid (SEQ ID NO.: 7) is 952 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 6-8 and ends with a TAA stop codon at nucleotides 945-947. A putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 5 A, and the start and stop codons are in bold letters. The representative ORF encodes a 313 amino acid polypeptide (SEQ ID NO.: 8) with a predicted molecular weight of 35077.8 Da. PSORT analysis of a NOV4 polypeptide predicts a plasma membrane protein with a certainty of 0.6000. Using SIGNALP analysis, it is predicted that a NOV4 polypeptide has no signal peptide. A NOV4 nucleic acid was cloned according to the method in Example 2.
TABLE 5A. TAATCATGTCCATTATCAACACATCATATGTTGAAATCACCACCTTCTTCTTGGTT GGGATGCCAGGGCTAGAATATGCACACATCTGGATCTCTATCCCCATCTGCAGCA TGTATCTTATTGCTATTCTAGGAAATGGCACCATTCTTTTTATCATCAAGACAGAG CCCTCCTTGCATGGGCCCATGTACTATTTTCTTTCCATGTTGGCTATGTCAGACTTG GGTTTGTCTTTATCATCTCTGCCCACTGTGTTAAGCATCTTCCTGTTCAATGCTCCT GAAACTTCATCCAATGCCTGCTTTGCCCAGGAATTCTTCATTCATGGATTCTCAGT ACTGGAGTCCTCAGTCCTCCTGATCATGTCATTTGATAGATTCCTAGCCATCCACA ATCCTCTGAGATACACCTCAATCCTGACAACTGTCAGAGTTGCCCAAATAGGGAT AGTATTCTCCTTTAAGAGCATGCTCCTGGTTCTTCCCTTCCCTTTCACTTTAAGAAG CTTGAGATATTGCAAGAAAAACCAATTATCCCATTCCTACTGTCTCCACCAGGATG TCATGAAGTTGGCCTGTTCTGACAACAGAATTGATGTTATCTATGGCTTTTTTGGA GCACTCTGCCTTATGGTAGACTTTATTCTCATTGCTGTGTCTTACACCCTGATCCTC AAGACTGTACCGGGAATTGCATCCAAAAAGGAGCAGCTTAAGGCTCTCAATACTT GTGTTTCACACATCTGTGCAGTGATCATCTTCTACCTGCCCATCATCAACCTGGCC GTTGTCCACCGCTTTGCCCGGCATGTCTCTCCCCTCATTAATGTTCTCATGGCAAAT GTTCTCCTACTTGTACCTCCGCTGATGAAACCAATTGTTTATTGTGTAAAAACTAA ACAGATTAGAGTGAGAGTTGTAGCAAAATTGTGTCAATGGAAGATTTAACAGTA (SEQ LD NO.: 7)
MSILNTS YVEITTFFLVGMPGLEYAHIWISIPICSMYLIAILGNGTILFILKTEPSLHGPMY YFLSMLAMSDLGLSLSSLPTVLSLFLFNAPETSSNACFAQEFFIHGFSVLESSVLLLMSFD --^LAIHNPLRYTSILTTVRVAQIGIVFSFKSMLLVLPFPFTLRSLRYCKKNQLSHSYCLH QDVMKLACSDNRIDVΓYGFFGALCLMVDFILIAVSYTLILKTVPGIASKKEQLKALNTC VSHICAVIIFYLPIIRXLAVVI-IRFARH^ RVVAKLCQWKI (SEQ ID NO. : 8)
A NOV4 nucleic acid has homology (64% identity) to a Homo sapiens olfactory receptor mRNA (OLR2; GENBANK-ID: HPFH6OR/acc: X81445), as is shown in Table 5B. A NOV4 polypeptide has homology (51% identity, 71% similarity) with an olfactory receptor- like protein from Gallus gallus (GALl ; SPTREMBL-ACC: Q9YH55), as is shown in Table 5C. The global sequence homology (as defined by FASTA alignment with the full length sequence of this protein) is 49.518 % amino acid identity and 60.450 % amino acid similarity. In addition, this protein contains the following protein domains: 7 transmembrane receptor (rhodopsin family) (IPR000276) from amino acid residues 3 to 222 (as defined by Interpro).
TABLE 5B.
NOV4 : 1 TAATCATGTCCATTATCAACACAT-C-ATATG-TTGAAATC-ACCACCTTCTTCT-TGGT 55 l l llll l I I Mil II I I 111 II I I I II II III II I II
OLR2 : 1433 TCACCATGACGTTT-TCAA-ACGTTCCATAACATTCACACCTACAACATTCA-CTCTCGT 1489
NOV4 : 56 TGGGATGCCAGGGCTAGAATATGCACAC-ATCTGGATCTCTATCCCCATCTGCAGCATGT 114
I I I I I I I n i i n i n i i i n m i i m m n m n i
OLR2 : 1490 TGGCATCCCGGGACTGGAGGCAGAACATTATGTGGATATCCATCCCCTTCTGCCTGATAT 1549
NOV4 : 115 ATCTTATTG-CTATTCTAGGAAATGGCACCATTCTTTTTATCATCAAGACAGAGCCCTCC 173 i i i i i i 1 1 m m i m i m m m m i n i m
OLR2 : 1550 ACACCATCATCT-TTCCGGGAAATGGCATCATTCTTCACATCATCCGAATTGACTCTTCC 1608
NOV4 : 174 TTGCATGGGCCCATGTACTATTTTCTTTCCATGTTGGC-TATGTCAGACTTGGGTTTGTC 232
I I I II III III III III II I II Mill I I I I II I III III I I II
OLR2 : 1609 TTGCACCAACCCATGTACTATTTTCTGGCCATGCCGGCCTTTGTTGAACTTGG-TGTCTC 1667 NOV4: 233 TTTATCATCTCTGCCCACTGTGTTAAGCATCTTCCTGTTCAATGCTCCTGAAACTTCATC 292
I II I III I III I 111 MM MM II MM I 1 II I
OLR2: 1668 TGCTTCCACCATGCCCACTGTGTTAAGCATATTCCTCTTTGGCATTAACGATGTCAGTTT 1727
NOV4: 293 CAATGCCTGCTTTGC-CCAGGAATTCTTC-ATTCATGGATTCTCAGTACTGGAGTCCTCA 350
II II I l ll llll II lll ll ll III I I lllllll
OLR2 : 1728 TGGTGGTTGCCT-GCTCCAGATGTT-TTCTATGCACTCTTTCACTCTTATGGAGTCAGGT 1785
NOV4: 351 GTCCTCCTGATCATGTCATTTGATAGATTCCTAGCCATCCACAATCCTCTGAGATACACC 410
I II II III II I I II I I I I II I I I I III I I I II II I I I I II I
OLR2: 1786 GTCCTTCTGGCAATGTCAGTGGACCGCTTTGTGGCCATCTACAGCCCACTGCGCTACACA 1845
NOV4: 411 TCAATCCTGACAACTGTCAGAGTTGCCCAAATAGGGATAGT-ATTCTCCTTTAAGAGCAT 469
I I I I I I I 11 1 M M I III I I II II I
OLR2: 1846 ACCATTCTGACAATTGCCTGCATTTCTGGGATGGGTGCCGCCATTG-CCTTGCGCAGTGT 1904
NOV4: 470 GCTCCTGGTTCTTCC-CTTCCCTTTCACTTTAAGAAGCTTGAGATAT-TG-CAAGAAAAA 526
I I I Ml I I I I MM II I I I I II I I II I I I I I
OLR2: 1905 AATGCTTATGCTCCCACTGCTCTTTCTCCTGAGGCGTCTGCCTTTCTGTGGCCACAATAC 1964
NOV4: 527 CCAATTATCCCATTCCTACTGTCTCCAC-CAGGATGTCATGAAGTTGGCCTGTTCTGACA 585
II I I I II 1 I I I I I I II I I Ml I I I I I I I I MM I I II II II
OLR2: 1965 CC—TCA-CACACTCTTATTGCCTCCACTCAG-ATCTGATCAAATTGCCCTGTGGAGACA 2020
NOV4: 586 ACA-GAATTGATGTTATCTATGGCTTT-TTTGG-AGCACTCTGC-CTTATGG—TAGACT 639
II I II 111 I Ml I I III I I II I I I I I I II I MM
OLR2: 2021 -CACGCCCCAATAGCATCC-TGGCTCTATTTGTCATTAC-CTTCACATTTGGACTGGACT 2077
NOV4: 640 TTATTCT-CATTGCTGTGTCTTACACCCTGATCCTCAAGACTGTACCGGGAATTGCATCC 698
I I II I I III I ll lllll lllll ll I II III I I MM II I I
OLR2: 2078 T-ATTGTTCATTGTGGTTTCTTATGTGCTGATTCTTCATACAGTACTGGAAATAGCTTCT 2136
NOV4: 699 AAAA-AGGAGCAGCTTAAGGCTCTCAATACTTGTGTTTCACACATCTGTGCAGTGATCAT 757
I II I I I I III I I II II I I I III I I I I II II I III I I I I I I
OLR2: 2137 GGAGCAGG-GC-GTGGCAGGCACTCAACACATGTGTGTCGCACATATGTGCTGTGCTTGT 2194
NOV4: 758 CTTCTACCTGCCCATCATCAACCTGGCCGTTGTCCACCGCTTTGCCCGGCATGTCTCTCC 817
I Ml I MM I I llll lll II I I MM MM I I llllll l MM
OLR2: 2195 GTACTATGTGCCCATGATCAGCCTCTCC-TGATGCACCGCTTTGGACGGCATTTACCTCC 2253
NOV4: 818 CCTCATTAATGTTCTCATGGCAAATGTTCTCCTACTTGTA-CCTCCGCTGATGAAACCAA 876
I I I I I I I I I I I I I I I I l l l l l l l I I I I I I I I I I I I I
OLR2: 2254 ACTTTTCCAGACTGTCACGGCCAATGCTTACCT-CTTCTTTCCTCCTGTGGTCAACCCCA 2312
NOV4: 877 TTGTTTATTGTGTAAAAACTAAACAGATTAGAGTGAGAGTTGT 919 (SEQ ID NO. 46)
II I I I II II Ml II III I I II I II II Ml
OLR2 : 2313 TTGTCTATAGTATCAAAATCAAAGAAATTCGCAACAGCGTTGT 2355 (SEQ ID NO. 47)
TABLE 5C.
NOV4: 45 FFLVGMPGLEYAHI ISIPICSMYLIAILGNGTILFIIKTEPSLHGPMYYFLSMLAMSDL 224
1 1 1 + 11+ I 1+ +1 I ll + l + ll I I II I +++ I I I II I I I I II +1 I
GALl: 12 FLLAGLPGMAQFHH VFLPFGLMYLVAVLGNGTILLVVRVHRQLHQPMYYFLLMLATTDL 71 N0V4 225 GLSLSSLPTVLSIFLFNAPETSSNACFAQEFFIHGFSVLESSVLLIMSFDRFLAIHNPLR 404
M + M + I M M + I I I I I I I I I I I 1 + 1 I I I I I l + l I I ++ I I I I I
GALl 72 GLTLSTLPTVLRVFWLGAMEISFPACLIQMFCIHVFSFMESSVLLAMAFDRYVAICCPLR 131 N0V4 405 YTSILTTVRVAQIGIVFSFKSMLLVLPFPFTLRSLRYCKKNQLSHSYCLHQDVMKLACSD 584 l + l I I I 1III1I+ + I +11 I I +1+ + I I I I I I I I I+++I I l + l
GALl 132 YSSILTGARVAQIGLGIICRCTLSLLPLICLLT LPFCRSHVLSHPYCLHQDIIRLACTD 191 NOV4 585 NRIDVIYGFFGALCLMVDFILIAVSYTLILKTVPGIASKKEQLKALNTCVSHICAVIIFY 764
++ + I I 1 ++ M + I M + M + 1 + 1 1 I I I l + l I I I I I I I I I I I I l + l I I GALl 192 ATLNSLYGLILVLVAILDFVLIALSYIMIFRTVLGITSKEEQTKALNTCVSHFCAVLIFY 251 NOV4 765 LPIINLAVVHRFARHVSPLINVLMANVLLLVPPLMKPIVYCVKTKQIRVRVVAKLCQ 935
+ 1+ I+++M+ |+ 1+ + +11 II I I I I++ I++I +1 + 1 I ++ III GALl 252 IPLAGLSIIHRYGRNAPPISHAVMANVYLFVPPILNPVLYSMKSKAICKGLLRLLCQ 308 NOV4 ( SEQ ID NO . 48 ) GALl ( SEQ ID NO . 49 ) Where | indicates identity and + indicates similarity.
The possible novel SNP variants of NOV4 are shown in Table 5D. The PHRED score indicates the SNP base pair quality, where a minimum score of 23 is acceptable. The method of identifying and confirming novel SNPs is described in Example 3.
Table 5D.
82: T->G(11) 125218920(i), phred 40 125218923(f), phred 42 125219376(0, phred 40 125219632(i), phred 33 125219739(0, phred 33 125586244(0, phred 29 125586186(f), phred 34 125586110(0, phred 35 126544369(0, phred 45 125588716(0, phred 33 125219986(0, phred 37
91: C->T(11)
125218920(0, phred 37 125218923(0, phred 33 125219376(0, phred 37 125219632(0, phred 22 125219739(0. phred 37 125586244(0, phred 32 125586186(0. phred 25 125586110(0, phred 37 126544369(0. phred 37 125588716(0. phred 33 • 125219986(0, phred 37
150: C->G(10)
• 125218920(0, phred 45 • 125218923(f), phred 51
• 125219376(0, phred 38
• 125219632(0, phred 41
• 125219739(Tι. phred 51
• 125586244(0, phred 40 • 125586186(0, phred 45
• 125586110(0, phred 45
• 126544369(0. phred 40
• 125588716(0. phred 45
157: G->A(2)
• 125219739(0. phred 45
• 125586186(0. phred 45
246: G->C(10) • 125218920(0. phred 40
• 125218923(0. phred 45
• 125219376m. phred 42
• 125219632(0. phred 21
• 125219739(0, phred 45 • 125586244(0. phred 38
• 125586186(0. phred 32
• 125586110(0. phred 36
• 126544369(f). phred 45
• 125588716(0. phred 45
296: G->A(10)
• 125218920(0. phred 39
• 125218923(0. phred 36
• 125219376(0. phred 36 • 125219632(0. phred 36
• 125219739(0. phred 49
• 125586244(0. phred 36
• 125586186(0. phred 36
• 125586110(0. phred 39 • 126544369(0. phred 36
• 125588716(0. phred 36
406: A->G(2)
• 125586198(i). phred 38 • 125219755(0, phred 29
450: C->T(8)
• 125218920(0, phred 27
• 125218923(0, phred 24 • 125219376(0, phred 22
• 125219739(0, phred 29
• 125586244(0, phred 30
• 125586186(0. phred 19 • 126544369(0. phred 28
• 125530948(0. phred 27
562: A->G(5)
• 125531346(0, phred 29 • 125530963(0, phred 29
• 125531302(0, phred 49
• 125530948(0. phred 31
• 125531257(0. phred 24
662: C->T(6)
• 125531346(0. phred 36
• 125530963(0. phred 41
• 125531302(0. phred 37
• 125530948(0, phred 40 • 125531257(0. phred 40
• 126652213(0. phred 37
664: A->G(6)
• 125531346(0. phred 45 • 125530963(0. phred 41
• 125531302(0. phred 45
• 125530948(0. phred 45
• 125531257(0, phred 44
• 126652213(0. phred 45
667: A->T(6)
• 125531346(0, phred 37
• 125530963(0, phred 45
• 125531302(0, phred 45 • 125530948(0, phred 40
• 125531257(0. phred 45
• 126652213(0. phred 45
671: A->G(7) • 125531283(0. phred 38
• 126652328(0. phred 45
• 126652243(0. phred 37
• 125531218(0. phred 45
• 125531233(0. phred 51 • 125531199(0. phred 45
• 125531268(ii phred 39
679: G->A(6)
• 125531346(0. phred 45 • 125530963(0. phred 45
• 125531302(0, phred 45
• 125530948(0, phred 45
• 125531257(0. phred 45 • 126652213(0, phred 37
776: C->T(6)
• 125531346(0, plired 41
• 125531302(0. phred 41 • 125530948(0, phred 45
• 126652243(0. phred 36
• 125531257(0. phred 45
• 126652213(0, phred 45
820: C->A(4)
• 125531346(0, phred 37
• 125530948(0, phred 40
• 125531257(0. phred 41
• 126652213(0. phred 45
NOV4 expression was analyzed by TaqMan analysis. Results are shown in Example 4,
Table 14C demonstate that NOVl expression is higher in lung cancer (small cell), colon cancer, ovarian cancer, prostate cancer and uterine cancer as compared to normal tissue.RTQ-
PCR probe/primer sets generated against GPCRs amplify the corresponding genomic region because GPCRs are single exon genes, as shown in Example 4 at Table 16B. The sequence is moderately expressed in many tissues; it is slightly higher in brain regions, especially cerebral cortex, indicating a utility as a marker to identify neuronal derived tissues and cells.
NOV5 A NOV5 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins. A NOV5 nucleic acid is found on human chromosome 11. A NOV5 nucleic acid and its encoded polypeptide include the sequences shown in Table 6A. The disclosed nucleic acid (SEQ ID NO.: 9) is 885 nucleotides in length and contains an open reading frame (ORF) that begins with a GGG codon at nucleotides 2-4, which codes for the amino acid Glycine, and ends with a TAA stop codon at nucleotides 881-883. A putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 6A, and the start and stop codons are in bold letters. The representative ORF encodes a 293 amino acid polypeptide (SEQ ID NO.: 10). PSORT analysis of a NOV5 polypeptide predicts a plasma membrane protein with a certainty of 0.6400. SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 33 and 34 in SEQ LD NO.: 10.
TABLE 6A. AGGGCTAGAATATGCACACATCTGGATCTCTATCCCCATCTGCAGCATGTATCTTATTGC 60 TATTCTAGGAAATTGCACCATTCTTTTTATCATCAAGACAGAGCCCTCCTTGCATGAGCC 120 CATGTACTATTTTCTTTCCATGTTGGCTATGTCAGACTTGGGTTTGTCTTTATCATCTCT 180 GCCCACTGTGTTAAGCATCTTCCTGTTCAATGCTCCTGA-AATTTCATCCAATGCCTGCTT 240 TGCCCAGGAATTCTTCATTCATGGATTCTCAGTACTGGAGTCCTCAGTCCTCCTGATCAT 300 GTCATTTGATAGATTCCTAGCCATCCACAACCCTCTGAGATACACCTCCATCCTGACAAC 360 TGTCAGAGTTGCCCAAATAGGGATAGTATTCTCCTTTAAGAGCATGCTCCTGGTTCTTCC 420 CTTCCCTTTCACTTTAAGAAΛCTTGAGATATTGCAAGAAAAACCAATTATCCCATTCCTA 480 CTGTCTCCACCAGGATGTCATGAAGTTGGCCTGTTCTGACAACAGAATTGATGTTATCTA 540 TGGCTTTTTTGGAGCACTCTGCCTTATGGTAGACTTTATTCTCATTGCTGTGTCTTACAC 600 CCTGATCCTCAAGACTGTACTGGGAATTGCATCCAAAAAGGAGCAGCTTAAGGCTCTCAA 660 TACTTGTGTTTCACACATCTGTGCAGTGATCATCTTCTACCTGCCCATCATCAACCTGGC 720 CGTTGTCCACCGCTTTGCCCGGCATGTCTCTCCCCTCATTAATGTTCTCATGGCAAATGT 780 TCTCCTACTTGTACCTCCACTGATGAACCCAATTGTTTATTGTGTAAAAACTAAACAGAT 840 TAGAGTGAGAGTTGTAGCAAAATTGTGTCAACGGAAGATTTAACA 885 (SEQ LD NO.: 9)
GLEYAHIWISI ICSMYLIAILGNCTILFIΓKTEPSLHEPMYYFLSMLAMSDLGLSLSSL 60 PTVLSIFLFNAPEISSNACFAQEFFIHGFSVLESSVLLIMSFDPJFLAIHNPLRYTSILTT 120 VRVAQIGIVFSFKSMLLVLPFPFTLRNLRYCKKNQLSHSYCLHQD VTVIKLACSDNRIDVIY 180 GFFGALCLMVOFILIAVSYTLILKTVLGIASKKEQLKALNTCVSHICAVIIFYLPIINLA 240 EDRFAlfflVSPLINVLMANV^^ 293 (SEQ ID NO.:
10)
The reverse complement (SEQ ID NO. 47) of a NOV5 nucleic acid sequence (SEQ LD NO. 9) has high homology (98% identity) with a nucleic acid encoding a human olfactory receptor-like polypeptide (HUMl ; : SeqCalling Accession No. : 150205708), as is shown in Table 6B. A NOV5 polypeptide was found to have homology (52% identity, 72% similarity) to a Gallus gallus olfactory receptor-like protein COR3ΕETA (GALl; SPTREMBL-ACC: Q9YH55), as is shown in Table 6C.
TABLE 6B.
NOV5 885 TGTTAAATCTTCCGTTGACACAATTTTGCTACAACTCTCACTCTAATCTGTTTAGTTTTT 826
I I I II II II I I II I III III II II II I I II I I II 11 I III I III I I I I III Mill I I I
4 TGTTAAATCTTCCATTGACACAATTTTGCTACAACTCTCACTCTAATCTGTTTAGTTTTT 63
NOV5 825 ACACAATAAACAATTGGGTTCATCAGTGGAGGTACAAGTAGGAGAACATTTGCCATGAGA 766
I I 11 II II I III I II II Ml I I I I I III II III II III III III I III III I II I III
HUMl 64 ACACAATAAACAATTGGTTTCATCAGCGGAGGTACAAGTAGGAGAACATTTGCCATGAGA 123
NOV5 765 ACATTAATGAGGGGAGAGACATGCCGGGCAAAGCGGTGGACAACGGCCAGGTTGATGATG 706
I I I II II II III II I III I I III II I I I II I II II II II III I I I II Ml III III I I I HUMl 124 ACATTAATGAGGGGAGAGACATGCCCGGCAAAGCGGTGGACAACGGCCAGGTTGATGATG 183
NOV5 705 GGCAGGTAGAAGATGATCACTGCACAGATGTGTGAAACACAAGTATTGAGAGCCTTAAGC 646
I I III III I III III II Ml I I III I 111 III III III III III II I I Mill IIMI II
HUMl 184 GGCAGGTAGAAGATGATCACTGCACAGATGTGTGAAACACAAGTATTGAGAGCCTTAAGC 243 N0V5 645 TGCTCCTTTTTGGATGCAATTCCCAGTACAGTCTTGAGGATCAGGGTGTAAGACACAGCA 586
Mill I III I MM II II I I I II 1 III I I II I Mill 11 II I 1 I II I Mill I 111 II
HUMl 244 TGCTCCTTTTTGGATGCAATTCCCGGTACAGTCTTGAGGATCAGAGTGTAAGACACAGCA 303
NOV5 585 ATGAGAATAAAGTCTACCATAAGGCAGAGTGCTCCAAAAAA-GCCATAGATAACATCAAT 527
I Ml I Ml I II II II III III II Ml II III 1 I Mill 111 MUM I Mill I III II
HUMl 304 ATGAGAATAAAGTCTACCATAAGGCAGAGTGCTCCAAΆAΆAΆGCCATAGATAACATCAAT 363
NOV5 526 TCTGTTGTCAGAACAGGCCAACTTCATGACATCCTGGTGGAGACAGTAGGAATGGGATAA 467 i M I 1 1 n i I I i i n i n i 1 1 i i n i I I n i m m i n i i m I I 1 1 n m n i i i i
HUMl 364 TCTGTTGTCAGAACAGGCCAACTTCATGACATCCTGGTGGAGACAGTAGGAATGGGATAA 423
NOV5 466 TTGGTTTTTCTTGCAATATCTCAAGTTTCTTAAAGTGAΆAGGGAAGGGAΆGAACCAGGAG 407
III II I I I I I II II III I II I II I Ml III 11 I III Ml III I III II I Mill III III
HUMl 424 TTGGTTTTTCTTGCAATATCTCAAGTTTCTTAAAGTGAAΆGGGAAGGGAAGAACCAGGAG 483
NOV5 406 CATGCTCTTAAAGGAGAATACTATCCCTATTTGGGCAACTCTGACAGTTGTCAGGATGGA 347
Mill III II III I III I III I I II I II II I I MM I I Ml I II II
HUMl 484 CATGCTCTTAAAGGAGAATACTATCCCTATTTGGGCAACTCTGACAGTTGTCAGGATTGA 543
NOV5 346 GGTGTATCTCAGAGGGTTGTGGATGGCTAGGΆATCTATCAAATGACATGATCAGGAGGAC 287
I MM Ml I II II III II II II II II II I II III I III M II II I II III II I
HUMl 544 GGTGTATCTCAGAGGATTGTGGATGGCTAGGAATCTATCAAΆTGACATGATCAGGAGGAC 603
NOV5 286 TGAGGACTCCAGTACTGAGAATCCATGAATGAAGAATTCCTGGGCAAAGCAGGCATTGGA 227 I Ml I Ml Ml Ml II II III Ml I Ml III III III III I II I I M M MM Ml I II I
HUMl 604 TGAGGACTCCAGTACTGAGAATCCATGAATGAAGAATTCCTGGGCAAAGCAGGCATTGGA 663
NOV5 226 TGAΆATTTCAGGAGCATTGAACAGGAAGATGCTTAACACAGTGGGCAGAGATGATAAΆGA 167 III II III II III II I I III II I III M II I II II II I III I III I II I I III I I II II I
HUMl 664 TGAAΆTTTCAGGAGCATTGAACAGGAAGATGCTTAΆCACAGTGGGCΆGAGATGATAAAGA 723
NOV5 166 CAAACCCAAGTCTGACATAGCCAACATGGAAAGAAAATAGTACATGGGCTCATGCAAGGA 107
I I I M II III II II II I I I I III III II I I II I I I I II I I II I MM II Ml II I I II I I
HUMl 724 CAAACCCAAGTCTGACATAGCCAACATGGAAΆGAAAATAGTACATGGGCTCATGCAAGGA 783
NOV5 106 GGGCTCTGTCTTGATGATAAAAAGAATGGTGCAATTTCCTAGAATAGCAATAAGATACAT 47
I I I M II II II II II II III II I I II II I II I III II I II I I II II 11 MM III I I II
HUMl 784 GGGCTCTGTCTTGATGATAAAAAGAATGGTGCCATTTCCTAGAATAGCAΆTAAGATACAT 843
NOV5 46 GCTGCAGATGGGGATAGAGATCCAGATGTGTGCATATTCTAGCCC 2 (SEQ ID NO. 50) Ill I II II I I III III II III I II MM II I I II II I III I 111 I
HUMl: 844 GCTGCAGATGGGGATAGAGATCCAGATGTGTGCATATTCTAGCCC 888 (SEQ ID NO. 51)
TABLE 6C.
NOV5 1 GLEYAHI ISIPICSMYLIAILGNCTILFIIKTEPSLHEPMYYFLSMLAMSDLGLSLSSL 60
1+ I I+ +I I I l + l + l I I I I I +++ ll + llllll lll +llll + ll + l GALl 19 GMAQFHHWVFLPFGLMYLVAVLGNGTILLVVRVHRQLHQPMYYFLLMLATTDLGLTLSTL 78 NOV5 61 PTVLSIFLFNAPEISSNACFAQEFFIHGFSVLESSVLLIMSFDRFLAIHNPLRYTSILTT 120
II II +1 I I II II I I I I I I +1 I I I I I l + l I I++ 1 I II M + l I II GALl 79 PTVLRVF LGAMEISFPACLIQMFCIHVFSFMESSVLLAMAFDRYVAICCPLRYSSILTG 138 NOV5 121 VRVAQIGIVFSFKSMLLVLPFPFTLRNLRYCKKNQLSHSYCLHQDVMKLACSDNRIDVIY 180
I II I 11+ + I +1 I I I +1+ + I II II I I I I+++ 1 I l + l ++ +1 GALl 139 ARVAQIGLGIICRCTLSLLPLICLLT LPFCRSHVLSHPYCLHQDIIRLACTDATLNSLY 198 NOV5 181 GFFGALCLMVDFILIAVSYTLILKTVLGIASKKEQLKALNTCVSHICAVIIFYLPIINLA 240
I I ++I l + l I l + l I +1 +IIIII M + ll I I II I I I I I I I l + l I 1 + 1+ 1 + GALl 199 GLILVLVAILDFVLIALSYIMIFRTVLGITSKEEQTKALNTCVSHFCAVLIFYIPLAGLS 258 NOV5 241 VVHRFARHVSPLINVLMANVLLLVPPLMNPIVYCVKTKQIRVRWAKLCQRKI 293
++M+ 1+ 1+ + +11 II I II I++M++I +1 + 1 1 ++ I I I I GALl: 225599 IIIIHHRRYYGGRRNNAAPPPEISHAVMANVYLFVPPILNPVLYSMKSKAICKGLLRLLCQR 309
NOV5: (SEQ ID NO. 10)
GALl: (SEQ ID NO. 52)
Where | indicates identity and + indicates similarity.
NOV6
A NOV6 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins. A NOV6 nucleic acid is found on human chromosome 11. A NOV6 nucleic acid and its encoded polypeptide include the sequences shown in Table 7A. The disclosed nucleic acid (SEQ D NO.: 11) is 798 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 24-26 and ends with a TGA stop codon at nucleotides 780-782. A putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 7A, and the start and stop codons are in bold letters. The representative ORF encodes a 252 amino acid polypeptide (SEQ ID NO.: 12) with a predicted molecular weight of 28399.4 Da. PSORT analysis of a NOV6 polypeptide predicts a plasma membrane protein with a certainty of 0.6400. SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 61 and 62 in SEQ ID NO.: 12.
TABLE 7A.
ATAACAACACCTTGCATACGCCCATGTATGTTTTCCTTCTGACACTGGCT GTTGTGGACATCATCTGCACAACAAGCATCATACCGAAGATGCTGGGGA CCATGCTAACATCAGAAAATACCATTTCATATGCAGGCTGCATGTCCCA GCTCTTCTTGTTCACATGGTCTCTGGGAGCTGAGATGGTTCTCTTCACCA CCATGGCCTATGACCGCTATGTGGCCATTTGTTTCCCTCTTCATTACAGT ACTGTTATGAACCACCATATGTGTGTAGCCTTGCTCAGCATGGTCATGGC TATTGCAGTCACCAATTCCTGGGTGCACACAGCTCTTATCATGAGGTTGA CTTTCTGTGGGCCAAACACCATTGACCACTTCTTCTGTGAGATACCCCCA TTGCTGGCTTTGTCCTGTAGCCCTGTAAGAATCAATGAGGTGATGGTGTA TGTTGCTGATATTACCCTGGCCATAGGGGACTTTATTCTTACCTGCATCT CCTATGGTTTTATCATTGTTGCTATTCTCCGTATCCGCACAGTAGAAGGC AAGAGGAAGGCCTTCTCAACATGCTCATCTCATCTCACAGTGGTGACCC TTTACTATTCTCCTGTAATCTACACCTATATCCGCCCTGCTTCCAGCTATA CATTTGAAAGAGACAAGGTGGTAGCTGCACTCTATACTCTTGTGACTCCC ACATTAAACCCGATGGTGTACAGCTTCCAGAATAGGGAGATGCAGGCAG GAATTAGGAAGGTGTTTGCATTTCTGAAACACTAGTAGTTTCAACATGC AA (SEQ ED NO.: 11) MYVFLLTLAVVDIICTTSILPKMLGTMLTSENTISYAGCMSQLFLFTWSLGAEMVLFTT MAYDRYVAICFPLHYSTVMNTfflMCVALLSMVMA-ϊAVTNSWVHTALIMRLTFCGPNT roHFFCEIPPLLALSCSPVRINEVMVYVADITLAIGDFILTCISYGFirVAILR-[RT^ AFSTCSS--1LTVVTLYYSPVIYTYIRPASSYTFERDKVVAALYTLVTPTLNPIV1VYSFQNR EMQAGIRKVFAFLKH (SEQ LD NO.: 12)
A NOV6 nucleic acid has homology (67% identity) with a Mus musculus GPCR mRNA (MUS3; GENBANK-LD: AF102539), as is shown in Table 7B. A NOV6 polypeptide has homology (59% identity, 74% similarity) with a 223 amino acid residue olfactory receptor protein bom Mus musculus (OLR3; SPTREMBL-ACC: Q9Z1T8), as is shown in Table 7C. In addition, this protein contains a seven transmembrane receptor domain (rhodopsin family) (LPR000276) from amino acid residues 1 to 232 (as defined by Interpro).
TABLE 7B.
NOV6 5 CTGGCTGTTGTGGACATCATCTGCACAACAAGCATCATACCGAAGATGCTGGG-GACCAT 103
I I III III I I I I Ml I III I I I I I Ml Mill I I I MUS3 7 CTGGCCACCATGGATATTATCTGCACCTCCTCTGTGCTGCCCAAGGCGCTGGTTGGTC-T 65 NOV6 104 GCTAACATCAG-AAAATACCATTTCATATGCAGGCTGCATGTCCCAGCTCTTCTTGTTCA 162
Ml I I II II II HIM I I 1 I 111 II Ml I Mill I III II II I MUS3 66 ACTATC-TGAGGAAAACACCATCTCCTTTAAAGGGTGCATGGCCCAGCTCTTCTTCCTTG 124 NOV6 163 CATGGTCTCTGGGAGCTGAGATGGTTCTCTTCACCACCATGGCCTATGACCGCTATGTGG 222
II III II I III I I I II II I I II III II II II I 11111 III II I MUS3 125 TGTGGTCCTTGTCTTCAGAGCTGCTGCTGCTCACAGTCATGGCCTATGACCGCTATGTGG 184 NOV6 223 CCATTTG-TTTCCCTCTTCATTACAGTACTGTTATGAACCACCATATGTGTGTAGC-CTT 280
II M II II III I I I I I I II II II MU M II II I III M M MUS3 185 CCATCTGCTTTCCC-CTGCACTACAGCTCTAGAATGAGCCCACAGTTGTGTGGGGCTCTG 243 NOV6 281 GCTCA-GCATGGTCATGG-CTAT-TGCAGTCACCAATTCCTGGGTGCACACAGCTCTTAT 337
I I I I I I I I I I I I I I I I I I I I I I I I 1 I I I I I I I I I I MUS3 244 GC-CATGGGTG-T-ATGGTCCATCTGTGCTCTG-AATGCATCTATCAACACTGGTCTGAT 299 NOV6 338 CATGAGGTTGACTTTCTGTGGGCCAAACACCATTGACCACTTCTTCTGTGAGATACCCCC 397 I II II I II I II I I I I I I 1 111 II III II I II II I I I II I I I II I MUS3 300 GACACGGCTGTCATTCTGTGGACCCAAGGTCATCACCCACTTCTTCTGTGAGATTCCCCC 359 NOV6 398 ATTGCTGGCTT-TGTCCTGTAGCCCTGTAAGAATCAATGAGG-TGATGG-TGTATGTTGC 454
I I II II I I II III Mill I I II II I II I I II I II MUS3 360 ACTCCTT-CTGCTCTCCTGTAGCCCCACATACGTAAAC-AGCATTATGACTCTAA-TAGC 416 NOV6 455 TGATAT-TACCCTGGCCATAGGGGACTTTATTCTTACCTGCATCTCCTATGGTTTTATCA 513 l l l l l l I I I I 1 11 I I I I I M M I I I I I I I I I 1 I I I I MUS3 417 AGATGTCTTCTATGGAGGCATCA-ATTTTGTGCTTACCTTACTATCCTATGGCTGCATCA 475 NOV6 514 TTGTT-GCTATTCTCCGTATCCGCACAGTAGAAGGCAAGAGGAAGGCCTTCTCAACATGC 572
Ml II II I I II I I II I I II III Mill II MM III II II I II MUS3 476 TTGCCAGC-ATCCTGCGCATGCGTTCTGCTGAGGGCAAGAGGAAGGCCTTTTCTACCTGC 534 NOV6 573 TCATCTCATCTCACAGTGGTGACCCTTTACTATTCTCCTGTAATCTACACCTATATCCGC 632
Ml II II I II I III II I I I III I I I Ml I III II II I I I I I MUS3 535 TCATCCCACCTCATCGTGGTCTCTGTGTACTACTCATCTGTGTTCTGTGCCTATGTCAGC 594 NOV6 633 CCTGCTTCCAGCTATACATTTGAAAGAGACAAGGTGGTAGCTGCACTCTA-TACTC-TTG 690
II Ml I MM II II I llllll III II II 11 I I I lllll ll MUS3 595 CCTGCATCCAGCTATAGCCCAGAAAGAAGCAAAGT—TACCT-CTGTGTTGTACTCATTC 651 NOV6: 691 -TGACTCCCACATTAAAC 707 (SEQ ID NO. 53)
I I II II I III
MUS3: 652 CTCAGCCCAACCCTGAAC 669 (SEQ ID NO. 54)
TABLE 7C.
NOV6 8 LAVVDIICTTSIIPKMLGTMLTSENTISYAGCMSQLFLFT SLGAEMVLFTTMAYDRYVA 67
II +11 + 11 l + l I I I +++ II II 1+ I M + ll I I II +I++I I II I I II II OLR3 3 LATMDIVCTPSVIPKALIGLVSEENTISF GCMAQLFFLL SLSSELLLLTVMAYDRYVA 62 NOV6 68 ICFPLHYSTVMNHHMCVALLSMVMAIAVTNS VHTALIMRLTFCGPNTIDHFFCEIPPLL 127
II I I III 1+ 1+ +1 II l +l 1+ I I I 1+ I l + l I I I I I II II II II I OLR3 63 ICFPLHYSSRMSPQLCGALAVGV SICAVNASVHTGLMTRLSFCGPKVITHFFCEIPPLL 122 NOV6 128 ALSCSPVRINEVMVYVADITLAIGDFILTCISYGFIIVAILRIRTVEGKRKAFSTCSSHL 187
M ill M M I II +I+II +III II ++I I+I+ II I IIII1II I III OLR3 123 LLSCSPTYINSVMTLVADAFYGCINFVLTLLSYGCIIASVLRMRSAEGKRKAFSTCSSHL 182 NOV6 188 TVVTLYYSPVIYTYIRPASSYTFERDKVVAALYTLVTPTLN 228 (SEQ ID NO. 55)
I I++M I I 1+ I II 11+ II II + I I++++I I I I
OLR3 183 IVVSVYYSSVFCAYVSPASSYSPERSKVTSVLYSILSPTLN 223 (SEQ ID NO. 56)
Where | indicates identity and + indicates similarity.
NOV7 (AC026038_B, CG50341-02) A NOV7 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins. A NOV7 nucleic acid is found on human chromosome 11. A NOV7 nucleic acid and its encoded polypeptide include the sequences shown in Table 8 A. The disclosed nucleic acid (SEQ ID NO.: 13) is 995 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 21-23 and ends with a TAA stop codon at nucleotides 966-968. A putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 8A, and the start and stop codons are in bold letters. The representative ORF encodes a 315 amino acid polypeptide (SEQ ID NO.: 14) with a predicted molecular weight of 34934.2 Da. PSORT analysis of a NOV7 polypeptide predicts a plasma membrane protein with a certainty of 0.6000. SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 46 and 47 in SEQ ID NO.: 14.
TABLE 8A. GGCGCTTATAATTTTGAACTATGACCAATCAGACACAGATGATGGAATTCTTGCTT GTGAGATTTACTGAGAATTGGGTGCTCCTGAGGCTGCATGCTTTGCTCTTCTCACT GATCTACCTCACGGCTGTGCTGATGAATTTAGTCATCATTCTCCTCATGATTCTGG ACCATCGTCTCCACATGGCAATGTACTTTTTCCTCCGACATTTGTCCTTCTTAGACC TGTGTCTCATTTCTGCCACAGTCCCCAAATCCATCCTCAACTCTGTCGCCTCCACTG ACTCCATCTCCTTCCTGGGGTGTGTGTTGCAGCTCTTCTTGGTGGTACTGCTGGCTG GATCAGAGATTGGCATCCTTACTGCCATGTCCTATGACCGCTATGCTGCCATCTGC TGCCCCCTACACTGTGAGGCTGTCATGAGCAGAGGGCTCTGTGTCCAGTTGATGGC
TCTGTCCTGGCTCAACAGAGGGGCCTTGGGACTCTTGTACACAGCTGGAACATTCT
CTCTGAATTTTTATGGCTCTGATGAGCTACATCAGTTCTTCTGCGATGTCCCTGCCC
TACTAAAGCTCACTTGTTCTAAAGAACATGCCATCATTAGTGTCAGTGTGGCCATT
GGGGTCTGTTATGCATTTTCATGTTTAGTTTGCATTGTAGTTTCCTATGTGTACATT
TTCTCTGCTGTGTTAAGGATATCACAGAGACAGAGACAATCCAAAGCCTTTTCCAA
CTGTGTGCCTCACCTCATTGTTGTCACTGTGTTTCTTGTAACAGGTGCTGTTGCTTA
TTTAAAGCCAGGGTCTGATGCACCTTCTATTCTAGACTTGCTGGTGTCTGTGTTCTA
TTCTGTCGCACCTCCAACCTTGAACCCTGTTATCTACTGTCTGAAGAACAAGGACA
TTAAATCCGCTCTGAGTAAAGTCCTGTGGAATGTTAGAAGCAGTGGGGTAATGAA
AGATGACTAAAGTTGAAGATGGGAAGTACTTTTTTTG (SEQ ID NO.: 13)
MTNQTQMMEFLLVRFTENWVLLRLHALLFSLIYLTAVLMNLVIILLMILDIiRLHlViAM YFFLRHLSFLDLCLISATVPKSILNSVASTDSISFLGCVLQLFLVVLLAGSEIGILTAMSY DRYAAICCPLHCEAVMSRGLCVQLMALS WLNRGALGLLYTAGTFSLNFYGSDELHQ FFCDVPALLKLTCSKEHAIISVSVAIGVCYAFSCLVCIVVSYVYIFSAVLRISQRQRQSK AFSNCWHLIVVTVFLVTGAVAYLKPGSDAPSILDLLVSVFYSVAPPTLNPVIYCLKNK DLKSALSKVLWNVRSSGVMKDD (SEQ LD NO.: 14)
A NOV7 nucleic acid has homology (38% identity) with a Pan tro lodytes GPCR mRNA (PAN1; GENBANK-ID: AF101730), as is shown in Table 8B. ANOV7 polypeptide has homology (49% identity, 64% similarity) with an olfactory-like receptor protein from Homo sapiens (OLR3; SPTREMBL-ACC: CAB65799), as is shown in Table 8C. In addition, this protein contains a seven transmembrane receptor domain (rhodopsin family) (LPR000276) from amino acid residues 40 to 149 (as defined by Interpro).
TABLE 8B.
NOV7 23 GACCAA-TCAGACACAG-ATGATGGAATTCTTGCTTGTGAGATTTACTGAGAATTGGGTG 80
M l I I I I I I I I I I I I I I I I I M l I 1 1 1 1 I I I I OLR3 8 GACAAAATCAAAC-CAGCATCTCAGACTTCCTGCTCCTGGGCCTGCCCATCCAACCAGAG 66 NOV7 81 CTCCTGAGGCTGCATGCTTTGCTCTTCTCACTG—ATCTACCTCACGGCTGTGCTGATGA 138
I I I III I II I I I I I I II I II I I I III I I I II I II OLR3 67 CAGCAAAACCTG—TGCTATGCCCTGTTCTTGGCCATGTATCTCACCACCCTCCTGGGGA 124 NOV7 139 ATTTAGTCATCATTCTCCTCATGATTCTGGACCATCGTCTCCACATGGCAATGTACTTTT 198
I I I II II I I I II II II 1 I I I I II III I I I III I I I I OLR3 125 ACCTCCTCATCATTGTCCTCATTCGACTGGACTCCCATCTCCACACGCCTATGTATTTGT 184 NOV7 199 TCCTCCGACATTTGTCCTTCTTAGACCTGTGTCTCATTTCTGCCACAGTCCCCAAATCCA 258
I I I I I I I III II MM lllll ll II III I II 1 111 I I I OLR3 185 TTCTCAGCAACTTGTCCTTCTCTGACCTCTGCTTCTCTTCCGTGACCATTCCCAAGTTGT 244 NOV7 259 TCCTCAACTCTGTCG—CCTCCACTGACTCCATCTCCT-TCCTGGGGTGTGTGTTGCAGC 315
I I III II I II II 1 1 III I I I II I I II M II II OLR3 245 TACAGAACA-TGCAGAACCAGGACCCA-TCCATCCCCTATGC-GGACTGCCTGACCCAAA 301 NOV7 316 TCTTCTTGGTGGTACTGCTGGCTGGATCAG-AGATTGGCATCCTTACTGCCATGTCCTAT 374
M i l l I I I I I I l l l l l l l M U M I I I I I I I I I I I OLR3 302 TGTACTTCTTCCTATTATTTGGAG-ACCTGGAGAGCTTCCTCCTTGTGGCCATGGCCTAT 360 NOV7 375 GACCGCTATGCTGCCATCTGCTGCCCCCTACACTGTGAGGCTGTCATGAGCAGAGGGCTC 434 II III I I I II II I II I II I I I I I II I I I I I II I III I III II I I
OLR3 361 GACCGCTATGTGGCCATCTGCTTCCCCCTGCACTACACCGCCATCATGAGCCCCATGCTC 420 NOV7 435 TGTGTC-CAGTTGATGGCTCTGTCCTGGCTCAACAGAGGGGCCTTGGGACTCTTGT-ACA 492
III II I I 1 1 I 111 I I II I I I II I I II II II l lll lll OLR3 421 TGTCTCTCCGTGG-TGGCGCTGTCCTGGGTG—CTGACCA-CCTTCCATGCCATGTTACA 476 NOV7 493 CAGCTGGAA-CATTCTCTC-T-GAATTTTTATGGCTCTGATGAGCTACATCAGTTCTTCT 549
I I I I I I I I I I I l l l l l l l l l l l l l I I I I I I I I OLR3 477 CA-CTTTACTCATGGCCAGGTTGTGTTTTTGTGCAGACAATGTGATCCCCCACTTTTTCT 535 NOV7 550 GCGATGTCCCTGCCCTACTAAAGCTCACTTGTTCTAAAGAACATGCCA-TCATTAGTGTC 608
I I II I II I I II I I III II l ll lll l I I l l l l l ll OLR3 536 GTGATATGTCTGCTCTGCTGAAGCTGGCCTGCTCTGACACTCGAGTTAATGAATGG-GTG 594 NOV7 609 AGTGTGGCCATTGG-GGTCTGTTATGCATTTTCATGT—TTAGTTTGCATTGTAGTTTCC 665
I I Ml M M II I I M I I I I I II I Ml I I Ml OLR3 595 ATATTTATCATGGGAGGGCTCAT-T-C-TTGTCATCCCATTCCTACTCATCCTTGGGTCC 651 NOV7 666 TATGTGTACATTTTCTCTGCTGTGTT-AAGGATATCA-CAGAGACAGAGACAATCCAAAG 723
I II I lll llll I I I II I I I I 1 I II I I I I I I OLR3 652 TATGCAAGAATTGTCTCCTCCATCCTCAAGG-TCCCTTCTTCTA-AGGGTATCTGCAAGG 709 NOV7 724 CCTTTTCCAACTGTGTGC-CTCACCTCATTGT—TGTCACTGTGTTTCTTGTAACAGGTG 780 llll ll l 11 I I II I II I I II 11 I I M II III I I I I I I I I I OLR3 710 CCTTGTCTACTTGTG-GCTCCCACCTCTCTGTGGTGTCACTGT-TCTATGGGACC-GTTA 766 N0V7 781 CTGTTGCT-TATTTAAAGCCAGGGTCTGATGCACCTTCTATTCTAGACT-TGCTGGTGTC 838
I I I I I I I I II III II I I I I I I I 111 II I II I OLR3 767 TTGGT-CTCTACTTATGCCCATCAGCTAATAGTTCTACTCTAAAGGACACTG-TCATGGC 824 NOV7 839 TGTGTTCTATTCTGTCGC-ACCTCCAACCTTGAACCCTGTTATCTACTGTCTGAAGAACA 897
I I I I II mi l III III ii mm i nm m mi l 1111 OLR3 825 TATGATATACACTGTGGTGACCCCCATGCT-GAACCCCTTCATCTACAGCCTGAGGAACA 883 NOV7 898 AGGACATTAAATCCGCTCTGAGTAAAGTCCT 928 (SEQ ID NO. 57) OLR3 884 GAGACATGAAGGGAGCCCTGAGCAGAGTCAT 914 (SEQ ID NO. 58)
TABLE 8C.
NOV7 1 MTNQTQMMEFLLVRFTEN VLLRLHALLFSLIYLTAVLMNLVIILLMILDHRLHMAMYFF 60
M M III+ I++ I I I I l + l + I I 1+ M + ll ++ +1 111 I l + l PAN1 1 MVNLTSMSGFLLMGFSDERKLQILHALVFLVTYLLALTGNLLIITIITVDRRLHSPMYYF 60 NOV7 61 LRHLSFLDLCLISATVPKSILNSVASTDSISFLGCVLQLFLVVLLAGSEIGILTAMSYDR 120 l + l I I I III II I M + ll 11+ II + l + l l + l + 11 I 1+ II I II II I PAN1 61 LKHLSLLDLCFISVTVPQSIANSLMGNGYISLVQCILQVFFFIALASSEVAILTVMSYDR 120 NOV7 121 YAAICCPLHCEAVMSRGLCVQLMALS LNRGALGLLYTAGTFSLNFYGSDELHQFFCDVP 180
MIM MM +I I + 1+ I M++ I 11+ I +1 I I I I I I I PAN1 121 YAAICQPLHYETIMDPRACRHAVIAV IAGGLSGLMHAAINFSIPLCGKRVIHQFFCDVP 180 NOV7 181 ALLKLTCSKEHAIISVSVAIGVCYAFSCLVCIVVSYVYIFSAVLRISQRQRQSKAFSNCV 240 + 111 II I I M M+ M + M+ IM MM + ++I IM + PAN1 181 QMLKLACSYEFINEIALAAFTTSAAFICLISIVLSYIRIFSTVLRIPSAEGRTKVFSTCL 240 NOV7 241 PHLIVVTVFLVTGAVAYLKPGSDAPSILDLLVSVFYSVAPPTLNPVIYCLKNKDIKSALS 300 lll l l ll +1+ I 1+ I +11+ I I I l + l I I I I I I I I I l + l +1 + 1 I PAN1 241 PHLFVATFFLSAAGFEFLRLPSDSSSTVDLVFSVFYTVIPPTLNPVIYSLRNDSMKAALR 300 NOV7 301 KVL 303 (SEQ ID NO. 59) l + l PA 1 301 KML 303 (SEQ ID NO. 60)
Where | indicates identity and + indicates similarity. One or more consensus positions of NOV7 have been identified as SNPs as shown in Table 8D. "Depth" represents the number of clones covering the region of the SNP. The Putative Allele Frequency (Putative Allele Freq.) is the fraction of all the clones containing the SNP. The sign ">" means "is changed to".
Table 8D.
NOV7 expression was analyzed by TaqMan analysis. Results are shown in Example 4, Table 17B, 17C and 17E. Tissue expression of NOV7 (AC026038_B) is overall at a low level in many tissues, as shown in Example 4 at Table 17B. The highest expression is seen in testis. Thus, NOV7 (AC026038_B) may be used as a marker for male germ cells and may have therapeutic applications in fertility disorders as a potential target. NOV7 (AC026038_B) is overexpressed in tumors derived from tissues responsive to steroid hormones- ovarian, uterine and prostate cancers as shown in Example 4 at Table 17C (Panel 2D). It is therefore a marker for cells, especially tumor cells responsive to steroid hormones. It can therefore be used to differentiate hormone-responsive and non-hormone responsive tumors, that are known to lead to different clinical outcomes. Being a GPCR, the protein can be used to screen candidate therapeutics for molecules able to modulate tumor growth, preferably small molecule therapeutic and human monoclonal antibodies. The pattern of expression in Tables 17C and 17E (Panel 4D) shows that Agl201 may have a potential role in inflammation, since NOV7 is expressed in activated basophils. Basophils are one of the key cell mediators of inflammation during asthma and allergy (lfnmunopharmacolo y 2000 Jul 25;48(3):269-81. Immunologically mediated signaling in basophils and mast cells: finding therapeutic targets for allergic diseases in the human Fcvar epsilonRl signaling pathway. Oliver JM, Kepley CL, Ortega E, Wilson BS.). This molecule may be important in allowing these cells to extravasate into the site of inflammation and/or in the activation of these cells. Antibody therapeutics to Agl201 may inhibit nasal and lung inflammation due to basophil activation and effectively reduce or eliminate symptoms of asthma, emphysema, and allergic rhinitis.
The high affinity IgE receptor, Fcvar epsilon RI, plays key roles in an array of acute and chronic human allergic reactions including asthma, allergic rhinitis, atopic dermatitis, urticaria and anaphylaxis. In humans and rodents, this receptor is found at high levels on basophils and mast cells where its activation by IgE and multivalent antigen produces mediators and cytokines responsible for Fcvar epsilon Rl-dependent acute inflammation. Mast cells can additionally contribute to sustained inflammatory responses by internalizing antigen bound to IgE-Fcvar epsilon RI complexes for processing to peptides and presentation to T cells. In humans, the Fcvar epsilon RI is also expressed, at lower density, on monocytes, macrophages and dendritic cells (DC), where its likely functions again include both signaling to mediator and cytokine production and antigen presentation. There have been studies focused on defining the earliest steps in the Fcvar epsilon RI signaling cascade in basophils and mast cells and on developing new routes to control allergic inflammation based on inhibiting these events. Antigen-stimulated Fcvar epsilon RI signaling may be limited by: (1) sequestering the Fcvar epsilon Rl-associated protein-tyrosine kinase, Lyn, that initiates FcvarepsilonRIsignaling; (2) eliminating; or (3) inactivating the protein-tyrosine kinase, Syk, that propagates FcvarepsilonRI signaling; and (4) establishing inhibitory crosstalk between Fcvar epsilon RI and a co-expressed receptor, FcgammaRII, that again limits Fc varepsilonRI- mediated Syk activation. These strategies may form the basis for new therapies for allergic inflammation.
NOV8 A NOV8 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins. A NOV8 nucleic acid is found on human chromosome 11. A NOV8 nucleic acid and its encoded polypeptide include the sequences shown in Table 9A. The disclosed nucleic acid (SEQ ID NO.: 15) is 969 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 27-29 and ends with a TAA stop codon at nucleotides 951-953. A putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 9A, and the start and stop codons are in bold letters. The representative ORF encodes a 308 amino acid polypeptide (SEQ LD NO.: 16) with a predicted molecular weight of 33960.9 Da. PSORT analysis of a NOV8 polypeptide predicts a plasma membrane protein with a certainty of 0.6400. SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 41 and 42 in SEQ LD NO.: 16.
TABLE 9A.
CTCTGTTTTCTGGTTTCAGTACCACAATGGACACAGGCAACAAAACTCTGCCCCA GGACTTTCTCTTACTGGGCTTTCCTGGTTCTCAAACTCTTCAGCTCTCTCTCTTTAT GCTTTTTCTGGTGATGTACATCCTCACAGTTAGTGGTAATGTGGCTATCTTGATGTT GGTGAGC ACCTCCC ATCAGTTGC ATACCCCC ATGTACTTCTTTCTGAGCAACCTCT CCTTCCTGGAGATTTGGTATACAACAGCAGCAGTGCCCAAAGCACTGGCCATCCT ACTGGGGAGAAGTCAGACCATATCATTTACAAGCTGTCTTTTGCAGATGTACTTTG TTTTCTCATTAGGCTGCACAGAGTACTTCCTCCTGGCAGCCATGGCTTATGACCGC TGTCTTGCCATCTGCTATCCTTTACACTACGGAGCCATCATGAGTAGCCTGCTCTC AGCGC AGCTGGCCCTGGGCTCCTGGGTGTGTGGTTTCGTGGCCATTGCAGTGCCCA CAGCCCTCATCAGTGGCCTGTCCTTCTGTGGCCCCCGTGCCATCAACCACTTCTTC TGTGACATTGCACCCTGGATTGCCCTGGCCTGCACCAACACACAGGCAGTAGAGC TTGTGGCCTTTGTGATTGCTGTTGTGGTTATCCTGAGTTCATGCCTCATCACCTTTG TCTCCTATGTGTACATCATCAGCACCATCCTCAGGATCCCCTCTGCCAGTGGCCGG AGCAAAGCCTTCTCCACGTGCTCCTCGCATCTCACCGTGGTGCTCATTTGGTATGG GTCCACAGTTTTCCTTCACGTCCGCACCTCTATCAAAGATGCCTTGGATCTGATCA AAGCTGTCCACGTCCTGAACACTGTGGTGACTCCAGTTTTAAACCCCTTCATCTAT ACGCTTCGTAATAAGGAAGTAAGAGAGACTCTGCTGAAGAAATGGAAGGGAAAA TAAATCTCCTCTACCACAA (SEQ ID NO.: 15)
MDTGNKTLPQDFLLLGFPGSQTLQLSLFMLFLVMYILTVSGNVAILMLVSTSHQLHTP MYFFLSNLSFLEIWYTTAAVPKALAILLGRSQTISFTSCLLQMYFVFSLGCTEYFLLAA MAYDRCLAICYPLHYGA-lMSSLLSAQLALGSWVCGFVAIA TALISGLSFCGPRArN HFFCDIAPWIALACTNTQAVELVAFVIAVVVILSSCLITFVSYVYIISTILRIPSASGRSKA FSTCSSHLTVVLIWYGSTVFLHVRTSIKDALDLIKAVHVLNTVVTPVLNPFIYTLRNKE VRETLLKKWKGK (SEQ ID NO.: 16)
A NOV8 nucleic acid has homology (71% identity) to a Rattus norvegicus GPCR mRNA (RAT1; GENBANK-ID: M64378) as is shown in Table 9B, and aNOV8 nucleic acid reverse complementary sequence has a high degree of homology (97% identity) to a nucleic acid encoding a human olfactory receptor-like polypeptide (HUM2; SeqCalling Accession No.:87740262) as is shown in Table 9C. A NOV8 polypeptide has homology (68% identity, 79%o similarity) to an olfactory-like receptor protein from Rattus norvegicus (OLR4; SPTREMBL-ACC: O95007), as is shown in Table 9D. The global sequence homology (as defined by GAP alignment with the full length sequence of this protein) is 67 % amino acid identity and 73 % amino acid similarity. In addition, this protein contains a seven transmembrane receptor domain (rhodopsin family) (LPR000276) from amino acid residue 41 to 290 (as defined by Interpro).
TABLE9B.
NOV8 28 TGGAC-ACAGGCAACAAAACTCTGC-C-CCAGGACT-TTC-TCTTACTGGGCTTTCCTGG 82
I II I II II I I II II I I I II I I II I 11 I I III II II III I I I I I RAT1 7 TGGAGTACTGGCCAGAAC-CTGTCCACACCAGGACCATTCATCTTGCTGGGCTTCCCAGG 65 NOV8 83 TTCTCAAACT-CTTCAGCTCTCT-CTCTTTATGCTTTTTCTGGTGATGTACATCCTCACA 140
I III I I II I III I I III Ml I II I II Ml II I I I I I RAT1 66 G-C-CAAGGAGCATGCGCATTGGGCTCTTCCTGCTTTTCCTGGTCATGTATCTGCTTACG 123 NOV8 141 GTTAGT-GGTAATGTGGCTATCTTGATGTTGGTGAGCACCTCCCATCAGT-TGCATACCC 198
II I I I I I M I I I I I I I I II I I I I I II I l ll ll l RAT1 124 GT-AGTTGGAAACCTAGCCATCATCTCCCTGGTAGGTGCC-CACAGATGCCTACAGACAC 181 NOV8 199 CCATGTACTTCTTTCTGAGCAACCTCTCCTTCCTGGAGATTTGGTATACAACAGCA-GCA 257
III II III II II I M III II I II I II I III II I II II MM II Mill II RAT1 182 CCATGTACTTCTTCCTCTGCAACCTCTCCTTCCTGGAGATCTGGTTCACCACAGCCTGC- 240 NOV8 258 GTGCCCAAAGCACTGGCCATCCTACTGGGGAGAAGTCAGAC-CATATCATTTACAAGCTG 316 II Mill l lllll II I I I M II Ml II II I MM RAT1 241 GTACCCAAGACCCTGGCCACATTTGCGCCTCGGGGTG-GAGTCATTTCCTTGGCTGGCTG 299 NOV8 317 TCTTTTGCAGATGTACTTTGTTTTCTCATTAGGCTGCACAGAGTACTTCCTCCTGGCAGC 376
I 1 I Mill III I I II I I II ll I ll I I I I I III I I I II II II II 1 1 RAT1 300 TGCCACACAGATGTACTTTGTCTTTTCTTTGGGCTGTACCGAGTACTTCCTGCTGGCTGT 359 NOV8 377 CATGGCTTATGACCGCTGTCTTGCCATCTGCTATCCTTTACACTACGGAGCCATCATGAG 436
I I I II M l Ml Ml I I II II II II I I I I I' I I I II I I I I MM I I I RAT1 360 GATGGCTTATGACCGCTACCTGGCCATCTGCCTGCCACTGCGCTATGGTGGCATCATGAC 419 NOV8 437 TAGCCTGCTCTCAGCGCAGCTGGCCCTGGGCTCCTGGGTGTGTGG-TTTCGTGGCCATTG 495
I III I II I III II III II I III I I III I II I I 11 II 1 1 I RAT1 420 TCCTGGGCTGGCGATGCGGTTGGCCCTGGGATCCTGGCTGTGTGGGTTTTCTG-CAATCA 478 NOV8 496 CAGTGCCCACAGCCCTCATCAGTGGCCTGTCCTTCTGTGGCCCCCGTGCCATCAACCACT 555
II II II I II II III III I I I I Ml III II I I II I II Ml Ml Ml RAT1 479 CAGTTCCTGCTACCCTCATTGCCCGCCTCTCTTTCTGTGGCTCACGTGTCATCAACCACT 538 NOV8 556 TCTTCTGTGACATTGCACCCTGGATTGCCCTGGCCTGCACCAACACACAGGCAGTAGAGC 615
II I ill II I Mill I I III III I I II III II II I I II I I II I I I I I I RAT1 539 TCTTCTGTGACATTTCGCCCTGGATAGTGCTTTCCTGCACCGACACGCAGGTGGTGGAAC 598 NOV8 616 TTGTGGCCTTTGTGATTGCTGT-TGTGGTTATCCTGAGTTCATGCCTCATCACCTTTGTC 67
I I I I I Mill Mill I ll I I I II I I II I I I I I I II II I Ml RAT1 599 TGGTGTCCTTTGGCATTGCCTTCTGTG-TTATTCTGGGCTCGTGTGGTATCACACTAGTC 657 NOV8 675 TCCTATGTGTACATCATCAGCACCATCCTCAGGATCCCCTCTGCCAGTGGCCGGAGCAAA 734 II III I I II II II II II II I Ml Ml Ml III II II II I MM II I RAT1 658 TCCTATGCTTACATCATCACTACCATCATCAAGATTCCCTCTGCCCGGGGCCGGCACCGC 717 NOV8 735 GCCTTCTCCACGTGCTCCTCGCATCTCACCGTGGTGCTCATTTGGTATGGGTCCACAGTT 794
II III III II II II I II I III II II Ml I I I I I III II I II II I Mill I RAT1 718 GCCTTCTCAACCTGCTCATCCCATCTCACTGTGGTGCTGATTTGGTATGGCTCCACCATC 777 NOV8 795 TTCCTTCACGTCCGCACCTCTATCAA-AGATGCCTTGGATCTGATCAAAGCTGTCCAC-G 852
Ml I II II I Mill M I II I Mill II M I II Mill M M I RATl 778 TTCTTGCATGTGAGGACCTCGGTAGAGAGCT-CCTTGGACCTCACCAAAGCTATC-ACAG 835 NOV8 853 TCCTGAACACTGTGGTGACTCCAGTTTTAAACCCCTTCATCTATACGCTTCGTAATAAGG 912 I I I I II II I I I I I I I II I I II II II II I I Ml I II l ll llll RATl 836 TGCTCAACACCATTGTCACACCTGTGCTGAACCCTTTCATATATACTCTGAGGAACAAGG 895 NOV8 : 913 AAGT-AAGAGAGACTCTGCTGAAGAAATG-GAAGGGAAAATAA 953 ( SEQ ID NO . 61 ) l l l l l l I I M M I I I I I I M i l l I I I 1 1 I
RATl: 896 ATGTCAAGGAAG-CTCTGC-GCAGGACGGTGAAGGGGAAGTGA 936 (SEQ ID NO. 62)
TABLE 9C.
NOV8 969 TTGTGGTAGAGGAGATTTATTTTCCCTTCCATTTCTTCAGCAGAGTCTCTCTTACTTCCT 910
M i l l M M I M i l l M i l l I I I I I I 1 I I I I I I I M i l l I M i l l M M I I HUM2 11 TTGTGGTAGAGGAGATTTATTTTCCCTTCCATTTCTTCAGCAGAGTCTCTCTTACTTCCT 70 NOV8 909 TATTACGAAGCGTATAGATGAAGGGGTTTAAAACTGGAGTCACCACAGTGTTCAGGACGT 850 II II I III II 111 II II I II II II II II I 11 I II II II II II II II II I II III II II II HUM2 71 TATTACGAAGCGTATAGATGAAGGGGTTTAAAACTGGAGTCACCACAGTGTTCAGGACGT 130 NOV8 849 GGACAGCTTTGATCAGATCCAAGGCATCTTTGATAGAGGTGCGGACGTGAAGGAAAACTG 790
I III 1 I I I I 11 II I 11 III Mill II II I III III I I II Mill I II 1 I MM II I II I 1 HUM2 131 GGACAGCTTTGATCAGATCCAAGGCATCTTTGATAGAGGTGCGGACGTGAAGGAAAACTG 190 NOV8 789 TGGACCCATACCAAATGAGCACCACGGTGAGATGCGAGGAGCACGTGGAGAAGGCTTTGC 730
I III I Ml I III Mill I II MM 11 II I III 11 MM 11 III Mill llllll Ml II I HUM2 191 TGGACCCATACCAAATGAGCACCACGGTGAGATGCGAGGAGCACGTGGAGAAGGCTTTGC 250 NOV8 729 TCCGGCCACTGGCAGAGGGGATCCTGAGGATGGTGCTGAT-GATGTACACATAGGAG 674
II I I I II II II II Mill 11 I II I MM I Ml II III II I 11 I II I III I HUM2 251 TCCGGCCACTGGCAGAGGGGATCCTGAGGATGGTGCTCATTGATGG-CAGA-AGGGG 305 NOV8 (SEQ ID NO. 63) HUM2 (SEQ ID NO. 64)
TABLE 9D.
NOV8 3 TG-NKTLPQDFLLLGFPGSQTLQLSLFMLFLVMYILTVSGNVAILMLVSTSHQLHTPMYF 61
I I I + I 1 + 1 1 I I I I +++++ I l + l I I I I l + l l l 1 1 + 1 1 + I I I M i l l OLR4 5 TGQNLSTPGPFILLGFPGPRSMRIGLFLLFLVMYLLTVVGNLAIISLVGAHRCLQTPMYF 64 NOV8 62 FLSNLSFLEIWYTTAAVPKALAILLGRSQTISFTSCLLQMYFVFSLGCTEYFLLAAMAYD 121
II I II II I 11 + 111 11 I I I I II I III Ml I II I 111 I II I II I I OLR4 65 FLCNLSFLEIWFTTACVPKTLATFAPRGGVISLAGCATQMYFVFSLGCTEYFLLAVMAYD 124 NOV8 122 RCLAICYPLHYGAIMSSLLSAQLALGSWVCGFVAIAVPTALISGLSFCGPRAINHFFCDI 181
I II II II 1 I I 1+ 1+ +1 I I I) l + lll I I I I 11+ 111 I I I II II II I I OLR4 125 RYLAICLPLRYGGIMTPGLAMRLALGSWLCGFSAITVPATLIARLSFCGSRVINHFFCDI 184 NOV8 182 AP IALACTNTQAVELVAFVIAVVVILSSCLITFVSYVYIISTILRIPSASGRSKAFSTC 241
+ 111 l + l l + l I I I I l + l II 111 II II III I I l + l I++I 1 I I II +IMM OLR4 185 SPWIVLSCTDTQVVELVSFGIAFCVILGSCGITLVSYAYIITTIIKIPSARGRHRAFSTC 244 NOV8 242 SSHLTVVLIWYGSTVFLHVRTSIKDALDLIKAVHVLNTVVTPVLNPFIYTLRNKEVRETL 301
I I I I I I I I I I I I I l + l I I I I I I++ +1 I I 11+ llll + lllllllllllllll + l + l I OLR4 245 SSHLTVVLI YGSTIFLHVRTSVESSLDLTKAITVLNTIVTPVLNPFIYTLRNKDVKEAL 304 NOV8 302 LKK KGK 308 (SEQ ID NO. 65)
+ III OLR4 305 RRTVKGK 311 (SEQ ID NO. 66)
Where | indicates identity and + indicates similarity.
NOV8 expression was analyzed by TaqMan analysis. A NOV8 nucleic acid is expressed in several tumor cell lines, including ovarian carcinoma OVCAR-8, and lung cancer (non-small cell) HOP-62, indicating a utility as a marker to identify tumor cells, preferably from ovarian and lung tumors, as shown in Example 4 at Table 18B. Being a GPCR, the protein can be used to screen for molecules able to modulate tumor growth, preferably small molecule therapeutics and human monoclonal antibodies. The expression in the adipose is tainted by genomic contamination. The expression in the testis indicates a utility to specifically identify male germ cells.
NOV9
A NOV9 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins. A NOV9 nucleic acid is found on human chromosome 11. A NOV9 nucleic acid and its encoded polypeptide include the sequences shown in Table 10A. The disclosed nucleic acid (SEQ ID NO.: 17) is 936 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 5-7 and ends with a TAA stop codon at nucleotides 929-931. A putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 10A, and the start and stop codons are in bold letters. The representative ORF encodes a 308 amino acid polypeptide (SEQ ID NO.: 18). PSORT analysis of a NOV9 polypeptide predicts a plasma membrane protein with a certainty of 0.6400. SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 41 and 42 in SEQ LD NO.: 18.
TABLE 10A.
TACAATGGACACAGGCAACAAAACTCTGCCCCAGGACTTTCTCTTACTGGGCTTTCCTGG 60 TTCTCAAACTCTTCAGCTCTCTCTCTTTATGCTTTTTCTGGTGATGTACATCCTCACAGT 120 TAGTGGTAATGTGGCTATCTTGATGTTGGTGAGCACCTCCCATCAGCTGCATACCCCCAT 180 GTACTTCTTTCTGAGCAACCTCTCCTTCCTGGAGATTTGGTATACCACAGCAGCAGTGCC 240 CAAAGCACTGGCCATCCTACTGGGGAGAAGTCAGACCATATCATTTACAAGCTGTCTTTT 300 GCAGATGTACTTTGTTTTCTCATTAGGCTGCACAGAGTACTTCCTCCTGGCAGCCATGGC 360 TTATGACCGCTGTCTTGCCATCTGCTATCCTTTACACTACGGAGCCATCATGAGTAGCCT 420 GCTCTCAGCGCAGCTGGCCCTGGGCTCCTGGGTGTGTG ΪTTTCGTGGCCATTGCAGTGCC 480 CACAGCCCTCATCAGTGGCCTGTCCTTCTGTGGCCCCCGTGCCATCAACCACTTCTTCTG 540 TGACATTGCACCCTGGATTGCCCTGGCCTGCACCAACACACAGGCAGTAGAGCTTGTGGC 600 CTTTGTGATTGCTGTTGTGGTTATCCTGAGTTCATGCCTCATCACCTTTGTCTCCTATGT 660 GTACATCATCAGCACCATCCTCAGGATCCCCTCTGCCAGTGGCCGGAGCAAAGCCTTCTC 720 CACGTGCTCCTCGCATCTCACCGTGGTGCTCATTTGGTATGGGTCCACAGTTTTCCTTCA 780 CGTCCGCACTTCTATCAAAGATGCCTTGGATCTGATCAAAGCTGTCCACGTCCTGAACAC 840 TGTGGTGACTCCAGTTTTAAACCCCTTCATCTATACGCTTCGTAATAAGGAAGTAAGAGA 900 GACTCTGCTGAAGAAATGGAAGGGAAAATAAATCTA 936 (SEQ LD NO.: 17)
MDTGNKTLPQDFLLLGFPGSQTLQLSLFMLFLVMYILTVSGNVAILMLVSTSHQLHTP MYFFLSNLSFLEIWYTTAAVPKALAILLGRSQTISFTSCLLQMYFVFSLGCTEYFLLAA MAYDRCLAICYPLHYGALMSSLLSAQLALGSWVCGFVAIAVPTALISGLSFCGPRALN IiFFCDIAPWLALACTNTQAVELVAFVIAVVVILSSCLITFVSYVYπSTILRLPSASGRSKA FSTCSSHLTVVLlN YGST LHVTlTSiKDALDLlKAVHVLNTVVTPVLNPFIYTLR VRETLLKKWKGK (SEQ LD NO.: 18)
A NOV9 nucleic acid has a high degree of homology (98% identity) to a Gorilla gorilla olfactory receptor mRNA (GORl ; GENBANK-ID: AF179756|acc: AF179756.1), as is shown in Table 10B. A NOV9 polypeptide has homology (68%> identity, 79% similarity) to an olfactory-like receptor protein from Rattus norvegicus (OLR5; SWISSPROT-ACC: P23267), as is shown in Table IOC. In addition, this protein contains a seven transmembrane receptor domain (rhodopsin family) (1PR000276) from amino acid residues 41 to 290 (as defined by Interpro).
TABLE 10B.
N0V9 373 TCTTGCCATCTGCTATCCTTTACACTACGGAGCCATCATGAGTAGCCTGCTCTCAGCGCA 432
Ml I II II I I I II I I Ml 11 I I I II II 11 Ml II 1 I I I II 11 I I 11 I I I 11 I II I 111 GORl 1 TCTTGCCATCTGCTATCCTTTACACTACGGAGCCATGATGAGTAGCCTGCTCTCAGTGCA 60 N0V9 433 GCTGGCCCTGGGCTCCTGGGTGTGTGGTTTCGTGGCCATTGCAGTGCCCACAGCCCTCAT 492
I I I Ml I I III I I I I MM I II I 11 III I I 1111 I I II I I II I I 11 II I 11 GORl 61 GTTGGCCCTGGGCTCCTGGGTTTGTGGTTTCATGGCCATTGCAGTGCCCACAGCCCTCAT 120 NOV9 493 CAGTGGCCTGTCCTTCTGTGGCCCCCGTGCCATCAACCACTTCTTCTGTGACATTGCACC 552
Ml I I I I I I I I II I III II III I Ml I I I II II III II I I III I II II I I I III III I 11 GORl 121 CAGTGGCCTGTCCTTCTGTGGCCCCCGTGCCATCAACCACTTCTTCTGTGACATTGCACC 180 NOV9 553 CTGGATTGCCCTGGCCTGCACCAACACACAGGCAGTAGAGCTTGTGGCCTTTGTGATTGC 612
I I I I I II I I II II I MM II II II I I I I I II II I II II I II I I MM I I I I III Ml I I 1 GORl 181 CTGGATTGCCCTGGCCTGCACCAACACACAGGCAGTAGAGCTTGTGGCCTTTGTGATTGC 240 NOV9 613 TGTTGTGGTTATCCTGAGTTCATGCCTCATCACCTTTGTCTCCTATGTGTACATCATCAG 672
II I I II I I I 1111 I I MM llll lll I II III I I 11 I III I I MM I I I Mill I I I II GORl 241 TGTTGTGGTTATCCTGAGTTCATGCCTCATCACCCTTGTCTCCTATGTGTACATCATCAG 300 NOV9 673 CACCATCCTCAGGATCCCCTCTGCCAGTGGCCGGAGCAAAGCCTTCTCCACGTGCTCCTC 732
II I III II II III II III II I III II III III II II I II III II III I II III III II II GORl 301 CACCATCCTCAGGATCCCCTCTGCCAGTGGCCGGAGCAAAGCCTTCTCCACGTGCTCCTC 360 NOV9 733 GCATCTCACCGTGGTGCTCATTTGGTATGGGTCCACAGTTTTCCTTCACGTCCGCACTTC 792
I I I III II III I II MM III III II III II III II I II I II III II II III III I II GORl 361 GCATCTCACCGTGGTGCTCATTTGGTATGGGTCCACAATTTTCCTTCACGTCCGCACCTC 420 NOV9 793 TATCAAAGATGCCTTGGATCTGATCAAAGCTGTCCACGTCCTGAACACTGTGGTGACTCC 852
I I 1 III I I I I I 1 Mill II III II III I II III 111 I III I II III I II III I I I II I I GORl 421 TATCAAAGACGCCTTGGATCTGATCAAAGCTGTCCACGTCCTGAACACTGTGGTGACTCC NOV9 853 AGTTTTAA 860 (SEQ ID NO. 67)
I I 1 II II I GORl 481 AGTTTTAA 488 (SEQ ID NO. 68)
Where | indicates identity.
TABLE IOC.
NOV9 3 TG-NKTLPQDFLLLGFPGSQTLQLSLFMLFLVMYILTVSGNVAILMLVSTSHQLHTPMYF 61
11 l + l l + l 11111 +++++ I l + l l I I I l + l I I 11 + 11+ I I l l l l l l OLR5 5 TGQNLSTPGPFILLGFPGPRSMRIGLFLLFLVMYLLTVVGNLAIISLVGAHRCLQTPMYF 64 NOV9 62 FLSNLSFLEI YTTAAVPKALAILLGRSQTISFTSCLLQMYFVFSLGCTEYFLLAAMAYD 121
I I III I 111 l + l I I III 11 I II 1 III II III 11 II III I I I III OLR5 65 FLCNLSFLEI FTTACVP TLATFAPRGGVISLAGCATQMYFVFSLGCTEYFLLAVMAYD 124 NOV9 122 RCLAICYPLHYGAIMSSLLSAQLALGS VCGFVAIAVPTALISGLSFCGPRAINHFFCDI 181
I I II I I I I I 11+ 1+ +1 I I II l + l 11 II I I 11+ II I I I I I I I I I II I OLR5 125 RYLAICLPLRYGGIMTPGLAMRLALGSWLCGFSAITVPATLIARLSFCGSRVINHFFCDI 184 NOV9 182 AP IALACTNTQAVELVAFVIAVVVILSSCLITFVSYVYIISTILRIPSASGRSKAFSTC 241
+ 111 l + l l + l I I I 1 l + l II III II 11 III I I l + l I++I I I I II +IIIII OLR5 185 SPWIVLSCTDTQVVELVSFGIAFCVILGSCGITLVSYAYIITTIIKIPSARGRHRAFSTC 244 NOV9 242 SSHLTVVLIWYGSTVFLHVRTSIKDALDLIKAVHVLNTWTPVLNPFIYTLRNKEVRETL 301
I I I I I I I I I I I I I l + l I I I II I++ +1 I I ll+ l l l l + l l l lllll l llll ll + l + l I OLR5 245 SSHLTWLI YGSTIFLHVRTSVESSLDLTKAITVLNTIVTPVLNPFIYTLRNKDVKEAL 304 NOV9 302 LKK G 308 (SEQ ID NO. 69)
+ 1 I I OLR5 305 RRTVKGK 311 (SEQ ID NO. 70)
Where indicates identity and + indicates similarity. NOV10
A NOV10 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins. A NOV10 nucleic acid is found on human chromosome 11. A NOV10 nucleic acid and its encoded polypeptide include the sequences shown in Table 11 A. The disclosed nucleic acid (SEQ LD NO.: 19) is 971 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 17-19 and ends with a TGA stop codon at nucleotides 950-952. A putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 11 A, and the start and stop codons are in bold letters. The representative ORF encodes a 311 amino acid polypeptide (SEQ LD NO.: 20) with a predicted molecular weight of 35147.4 Da. PSORT analysis of a NOV10 polypeptide predicts a plasma membrane protein with a certainty of 0.6000. SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 59 and 60 in SEQ ID NO.: 20.
TABLE 11 A.
CTCTCTTTAGGAAGAAATGGAACGACCACAAAGTGATTTTAACCAAACTGAAGTT
GCTGAATTTTTCCTCATGGGATTTTCGAATTCCTGGGATATTCAGATTGTACATGCT
GCTCTATTCTTCCTAGTTTACCTGGCAGCTGTCATAGGAAATCTCCTAATCATCAT ACTTACCACTCTGGATGTTCACCTCCAAACCCCAATGTATTTCTTTTTGAGAAACT TGTCTTTCTTAGATTTTTGTTACATCTCTGTCACAATTCCAAAATCTATTGTTAGTT CCTTGACTCATGATACTTCCATTTCTTTCTTTGGGTGTGCTCTGCAAGCCTTCTTTT TCATGGACTTGGCAACTACGGAGGTAGCCATCCTTACAGTGATGTCCTATGACCGC TATATGGCCATCTGCCGGCCTTTACATTATGAGGTCATCATAAACCAAGGTGTCTG TCTGAGGATGATGGCCATGTCGTGGCTCAGTGGGGTGATCTGTGGATTCATGCATG TGATAGCAACATTCTCATTACCATTCTGTGGGCGCAATAGAATACGTCAATTTTTC TGTAATATTCCACAGCTCCTAAGCCTCTTAGACCCCAAAGTAATTACCATTGAGAT TGGAGTCATGGTTTTTGGTACAAGTCTTGTGATAATCTCCTTTGTTGTAATTACTCT CTCCTACATGTACATTTTTTCTGTCATCATGAGGATTCCTTCTAAGGAGGGTAGAT CAAAAACATTTTCTACCTGCATTCCACATCTTGTGGTTGTAACACTCTTTATGATAT CTGGCAGCATTGCCTATGTGAAGCCAATTTCAAATTCTCCCCCCGTTCTGGATGTT TTCCTGTCTGCGTTCTACACAGTCGTGCCCCCGACCCTGAACCCCGTCATCTATAG TCTGAGGAATAGGGACATGAAGGCAGCCCTGAGAAGGCAGTGTGGTCCCTGAGA AGGCAGTGTGGTATGCT (SEQ ID NO.: 19)
MERPQSDFNQTEVAEFFLMGFSNSWDIQIVΉAALFFLVYLAAVIGNLLIΠLTTLDVHL QTPMYFFLRNLSFLDFCYISVTLPKSIVSSLTHDTSISFFGCALQAFFFMDLATTEVAILT VMSYDRYMAICRPLITYEVILNQGVCLRMM-AMSWLSGVICGFM--WIATFSLPFCGR-NR RQFFC-NILPQLLSLLDPKVITIEIGV-MVFGTSLVΠSFVVITLSYMYIFSVIMRPSK^ TFSTCIPHLVVVTLFMISGSIAYVKPISNSPPVLDVFLSAFYTVVPPTLNPVIYSLRNRD MKAALRRQCGP (SEQ LD NO.: 20) A NOV10 nucleic acid has homology (63% identity) to a Homo sapiens GPCR mRNA (GPCR2; GENBANK-LD: AL096770), as is shown in Table 1 IB. A NOV10 polypeptide has homology (51% identity, 68% similarity) to an olfactory receptor-like protein from Homo sapiens (OLR3; SPTREMBL-ACC: CAB65799), as is shown in Table 1 lC. The global sequence homology is 50.49% amino acid identity and 57.37% amino acid similarity. In addition, this protein contains a seven transmembrane receptor domain (rhodopsin family) (LPR000276) from amino acid residues 47 to 296 (as defined by Interpro).
TABLE 11B.
NOV10 956 CTTCTCAGGGACCACACTGCCTTCTCAGGGCTGCCTTCATGTCCCTATTCC-TCAGACTA 898
II I I I 111 II I I I 111 II 11 II I 111 I I II I I II I I I I II I GPCR2 81213 CTTC-CTTTGACAGCA-T-CTTCCTCAGTGCTGCCTTCATGGAATCATTCCGTAAG-CTA 81268 NOV10 897 TAGATGACGGGGTTCAGGGTCGGGGGCACGACTGTGTAGAACGCAGACAGGAAAACATCC 838
I I I II II 11 II I I I I I I II I I I I I I I I I I I I I I I II III GPCR2 81269 TAAATGACTGGATTGAGTGTTGGAGGTATCACAGTATAGAATACGGAGAATACAAGGTCC 81328 NOV10 837 AGAA-CGGGGGGAGAATTTGAAATTGGCTTCACATAGGCAATGC-TGCCAGATATCATAA 780
I I M l l llll II I I I I I I I II I I I II I I I 1 11 GPCR2 81329 ACAGTCGATGAG-GAATCAGAAGGCAGTCTGAGAAACTCAAAGCCTGC-AGCTGAΆAGAA 81386
NOV10: 779 AGAGTGTTACAACCACAAGATGTGGAATGCAGGTAGAAAATGTTTTTGATCTA-CCCTCC 721
I I I 1 1 I I I I 1 1 I I I 1 1 1 1 1 1 I I I 1 I I I I 1 1 I I I m i l
GPCR2: 81387 AGAAGGTGGCTACAAATAGGTGTGGTAGGCAGGTGGAGAA-GACCTTGGTCCGGCCCTCA 81445
NOV10: 720 TTAGAAGGAATCCTCATGA-TGACAGAAAAAATGTACATGTAGGAGAGAGTAATT-ACAA 663
I I 11 11 Ml I I II 111 II I I I I II 111 I I I II III I I GPCR2: 81446 GCTGATGGGATTCTCAGCACTGT-AGAGAAGATGCGAATGTAGGAGAGCACAATGGAGAT 81504
NOV10: 662 CAAAGGAGATTA-T-CACAAGAC-TTGTACCAAAAACCATGACTCCAATCTCAATGGT-A 607 n i i I I I I I I I m i m i i n i i i m u m i i i
GPCR2: 81505 CAAAC-AGATAAATGCTGCAGACGTTGTGAATGCAGCCA-G—TGCAATCTCATTAATGA 81560
NOV10: 606 ATTACTTTGGGGTCTAAGAGGCTTAGGAGCTGTGGAATATTACAGAAAAATTGACGTATT 547
III I I I 11 I I I I III III I 11 III III III I I I II GPCR2: 81561 ATT-CATAAGAACAGGCTAGTTTCAGCATCTGAGGAACATCACAGAAGAATTGGTGAATG 81619
NOV10: 546 -CTATTGCGCCCACAGAATGGTAATGAGAATGTTGCTATCA-CATGCATGAATCCACAGA 489 n i ni m 11 mi mu m i 11 m mi 11 m m
GPCR2: 81620 ACTCTCTT-CCCACAGAGAGGTATGGAGAA-GTTAATGGCAGCATGCATGAGCCCAGAGA 81677 NOV10: 488 TCACCCCACTGAGCCACGACATGGCCATCA-TCC-T-CAGACAGACACCTTGGTTTATGA 432 i mi i in i ni i i mi i i i i mi ni i i n i
GPCR2: 81678 GGCCCCCAGCAATCCAC-ACA-G-CTATCACTGCATGCCTACAGGCACGGGGATCCATAA 81734 NOV10: 431 TGACCTCATAATGTAAAGGCCGGCAGATGGCCATATAGCGGTCATAGGACATCACTGTAA 372
1 III 1 11 I 11111 11 II I nm I 111 II II III I I
GPCR2: 81735 TAGTCTCATAATGAAGTGGTTGACAGATTGCTGCGTACCTGTCATAAGACATCACTGTGA 81794
NOV10: 371 GGATGGCTACCTCCGTAGTTGCCA-AGTCCATGAAAAAGAAGGCTTGCAGAGCACACCCA 313
I I I III I Ml I I III Ml I I II II I III! l ll lll 111 1 GPCR2: 81795 GAATGGCGACTTCTGATGAGGCCAGAG-CTATGAAGAAGAAAACCTGAAGAATGCACTGA 81853
NOV10: 312 AAGAAAGAAATGGAAGTATCATGAGTCAAG-GAACTAACAATAGATTTTGGAATTGTGAC 254
1 I III I I 11 II I I 111 III I llll ll l II I GPCR2: 81854 ACAAGAGAAATGTAACCGTTGCCCAT-AAGTGAATTTGCAATGGACTGGGGGACTGTGAC 81912
NOV10: 253 AGAGATGTAACAAAAATCTAAGAA-AGACAAGTTTCTCAAAAAGAAATACATTGGGGTTT 195 n i i i i i i i i i i i m n n i i 1 1 i m i n i n m i i i n i
GPCR2: 81913 AGAGATGAAGCAGAGGTCCA-GAAGAGAGAGGTGCTTTAAAAAGTAATACATGGGGGAAT 81971 NOV10: 194 GGAGGTGAACATCCAGAGTGGTAAGTATGATGATTAGGAGATTTCCTATGACAGCTGCCA 135 GPCR2 81972 GGAGACGACGGTCCACGGTAATGATGGTGATAATGAGGAGGTTGCCTGTCAAGGCCAGCA 82031 NOV10 134 GGTAAACTAGGAAGAATAGAGCAGCATGTACAATCTGAATATCCCAGGAATTCGAAAATC 75 i m i i m i m i in in m n i i II i mi i GPCR2 82032 GGTATGTCACCAGAAATACCAATGCATGTAAAATCTGAAGCTTACGCTCATCAGAAAACC 82091 NOV10 74 CCATGAGGAAAAATTCAGCAACTTCAGT 47 (SEQ ID NO. 71) III I 11 I III 11 1 1 111 GPCR2 82092 CCATAAGAAGGAATCCACTCATTGAAGT 82119 (SEQ ID NO. 72)
TABLE llC.
NOV10: 9 NQTEVAEFFLMGFSNS DIQIVHAALFFLVYLAAVIGNLLIIILTTLDVHLQTPMYFFLR 68
I I ++ I I I I I 1+ +11 + 11 +1 + 11 1+ + 1 + 1 I +111 + 11 +
OLR3: 3 NLTSMSGFLLMGFSDERKLQILHALVFLVTYLLALTGNLLIITIITVDRRLHSPMYYFLK 62
NOV10: 69 NLSFLDFCYISVTIPKSIVSSLTHDTSISFFGCALQAFFFMDLATTEVAILTVMSYDRYM 128
+ 1 1 I I I + 1 I I I + 1 + 1 I + 1 1 + I I I I I 1 1 1 + M ++ M M I M M M M
OLR3: 63 HLSLLDLCFISVTVPQSIANSLMGNGYISLVQCILQVFFFIALASSEVAILTVMSYDRYA 122
NOV10: 129 AICRPLHYEVIINQGVCLRMMAMSWLSGVICGFMHVIATFSLPFCGRNRIRQFFCNIPQL 188
I I I + 1 1 1 I I I ++ I + I ++ I + I I I 1 1 + 1 1 1 + I I I I 1 ++ I 1 +
OLR3: 123 AICQPLHYETIMDPRACRHAVIAV IAGGLSGLMHAAINFSIPLCGKRVIHQFFCDVPQM 182
NOV10: 189 LSLLDPKVITIEIGVMVFGTSLVIISFVVITLSYMYIFSVIMRIPSKEGRSKTFSTCIPH 248
I I 11 + I II 1 + I I I 1+ I I I ++I I I I II l + l II I l + l I
OLR3: 183 LKLACSYEFINEIALAAFTTSAAFICLISIVLSYIRIFSTVLRIPSAEGRTKVFSTCLPH 242
NOV10: 249 LVVVTLFMISGSIAYVKPISNSPPVLDVFLSAFYTWPPTLNPVIYSLRNRDMKAALRR 307
I I 1 1+ + +++ l + l +1+ I I I I l + l I I I I I II I I I II II II I 1 +
OLR3: 243 LFVATFFLSAAGFEFLRLPSDSSSTVDLVFSVFYTVIPPTLNPVIYSLRNDSMKAALRK 301
NOV10: (SEQ ID NO. 73) OLR3: (SEQ ID NO. 74)
Where | indicates identity and + indicates similarity.
NOV10 expression was analyzed by TaqMan analysis, as is shown in Table 19B.
NOV11
A NOVl 1 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins. A NOVl 1 nucleic acid is found on human chromosome 11. A NOVl 1 nucleic acid and its encoded polypeptide include the sequences shown in Table 12A. The disclosed nucleic acid (SEQ ID NO.: 21) is 1010 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 37-39 and ends with a TAA stop codon at nucleotides 982-984. A putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 12A, and the start and stop codons are in bold letters. The representative ORF encodes a 315 amino acid polypeptide (SEQ ID NO.: 22) with a predicted molecular weight of 35620.1 Da. PSORT analysis of a NOVl 1 polypeptide predicts a plasma membrane protein with a certainty of 0.6000. SIGNALP analysis suggests the presence of a signal peptide with the two possible cleavage sites: one occuring between positions 49 and 50, and one between positions 59 and 60 in SEQ LD NO.: 22.
TABLE 12A.
GCTCTCATCTCTGATACAAGCCTTAAAGAAGGGTAAATGAGGCAGAATAACAATA
TTACAGAATTTGTCCTCCTGGGCTTCTCTCAGTATCCTGATGTGCAAAATGCATTA
TTTGTCATGTTTTTACTCATATATATTGTGACTATGGTGGGGAACCTGCTCATTGTG GTGTCTATTATTGCCAGTCCCTTTTTGGGCTCCCCAGTGTACTTCTTCCTTGCCTGC CTGTCATTTATAGATGCTGTGTATTCCACCACCATTTCTCCTGTATTGATTGTAGAC TTACTCTGTGATAAAAAGACTATTTCCTTCCCAGCTTGCATGGGTCAGCTATTTAT AGAGCACTTGTTTGGTGATACTGACGTCTTCCTTCTGGTGGTGATGGCCTATGATC GCTACGTGGCCACCTGTAAGCCACTGCGCTATTTGACCATCATGAATAGACAGGTT TGCATCCTTCTGTTGGTGGTGGCTGTGACTGGAGGTTTTCTGCATTCTGTGTTTCAA ATTTTAGTTGTGTACAGTCTCCCTTTCTGTGGCCCCAATGTCATTTATCACTTTTTC TGTAACATATACCCTTTATTGGACCTGGAATGCACTGACACCTACTTCGTAGGCCT CGCTGTGGTTTTCAATGGTGGAGCAATCTGTATGGTCATCTTCACCCTTCTACTAA TCTCCTATGGGGTCATCCTAAACTCCCTTAAAACTTATAGTCCGGAAGGGAGGCAT AAAGCTCCGTTTATCTGCAGCTCCCACTTTATCATGGTTATCTTGTTTTTTGTTCCC TGTATTTTCTTATATGTTAGACCCGTTTCAAACTTTCCTATTGATAAATTCCTGACT GTGTTTTATTCAGTTATCACACCCAAGTTGAATCCTTTTATATACATGTTGAGAAA TTCAGAGATGAGAAATGCTATAGAAAATCTCTTGGGATACCAAAGTGGGAAGACA GGATTTAGATGCTCCAAGCTCAATTAAGAAGTCATCCTATCTTGGCATCTGTT (SEQ ID NO.: 21)
MRQNNNITEFVLLGFSQYPDVQNALFVMFLLIYIVTMVGNLLΓVVSIIASPFLGSPVYFF LACLSFIDAVYSTTISPVLIVDLLCDKKTISFPACMGQLFIEHLFGDTDVFLLVVMAYD RYVATCKPLRYLTI NRQVCILLLVVAVTGGFLHSVFQILVVYSLPFCGPNV-YHFFCN IYPLLDLECTDTYFVGLAVVFNGGAICMV1FTLLLISYGVILNSLKTYSPEGRHKAPFIC SSOTIMVILFF CIFLYVRPVSNFPLDKFLTVFYSVITPKLNPFIYMLRNSEMRNAIENL LGYQSGKTGFRCSKLN (SEQ ID NO.: 22)
A NOVl 1 nucleic acid has homology (88% identity) to a Homo sapiens GPCR mRNA (GPCR3; GENBANK-ID: AF065869), as is shown in Table 12B. A NOVl 1 polypeptide has homology (69%> identity, 81% similarity) to an odorant receptor protein Rattus norvegicus (ODOR1; SPTREMBL-ACC: G264618), as is shown in Table 12C. The global sequence homology (as defined by GAP alignment with the full length sequence of this protein) is 48% amino acid identity and 57% amino acid similarity. In addition, this protein contains a seven transmembrane receptor domain (rhodopsin family) (LPR000276) from amino acid residues 39 to 285 (as defined by Interpro). TABLE 12B.
NOVll: 37 ATGAGGCAGAATAACAATATTACAGAATTTGTCCTCCTGGGCTTCTCTCAGTATCCTGAT 96
III II II II II II II M M III M II II II II Ml Ml Ml Ml II M I III II 1
GPCR3 : 1 ATGAGACAGAATAATAATATTACAGAATTTGTCCTCCTGGGCTTCTCTCAGGATCTGGAT 60
NOVll: 97 GTGCAAAATGCATTATTTGTCATGTTTTTACTCATATATATTGTGACTATGGTGGGGAAC 156
M I II M I M II II M II II II III M II Ml III I II II I MM I III III
GPCR3 : 61 GTGCAAAAAGCATTATTTGTCATATTTTTACTCACATACTTGGTGACAGTGGTGGGGAAC 120
NOVll: 157 CTGCTCATTGTGGTGTCTATTATTGCCAGTCCCTTT-TTGGGCTCCCCAGTGTACTTCTT 215
11 III II MM I 11 I I I I 11 III III I II II I I Mill III I 11 II II I III I I
GPCR3 : 121 CTGCTCATTGTGGTGACTATTATTACCAG-CCCTTCCTTGGGCTCCCCAATGTACTTCTT 179
NOVll: 216 CCTTGCCTGCCTGTCATTTATAGATGCTGTGTATTCCACCACCATTTCTCCTGTATTGAT 275
I I M M I I I I I I I I M l I I M i l l I I I I I I M l I I I 1 I I I 1 1 I I I I I l l l l l l
GPCR3 : 180 CCTTGCCTGCCTGTCATTTATAGATGCTGCATATTCCACTACAATTTCTCCCAAATTGAT 239
NOVll: 276 TGTAGACTTACTCTGTGATAAAAAGACTATTTCCTTCCCAGCTTGCATGGGTCAGCTATT 335
II II III I III II I I III II I Ml I Ml II I I I II II M III III II II I III II II
GPCR3 : 240 TGTAGACTTACTCTGTGATAAAAAGACTATTTCTTTCCCAGCTTGCATGGGCCAGTTATT 299
NOVll: 336 TATAGAGCACTTGTTTGGTGATACTGACGTCTTCCTTCTGGTGGTGATGGCCTATGATCG 395
III I I III III II II I I I I I II I I 11 II II I I II II I II II 11 MM Mill
GPCR3: 300 TATATACCACTTGTTTGGTGGTTCTGAGGTCTTCCTTCTTGTGGTGATGGCCTGTGATCA 359
NOVll: 396 CTACGTGGCCACCTGTAAGCCACTGCGCTATTTGACCATCATGAATAGACAGGTTTGCAT 455 n i i i I I i I I n i i I I i n m n m I I I I I I i i i n m n m 1 1
GPCR3 : 360 CTATGTGGCCATCTGTAAGCCACTGCACTATTTGACCATCATGAATCGACAGGTTTGAAT 419
NOVll: 456 CCTTCTGTTGGTGGTGGCTGTGACTGGAGGTTTTCTGCATTCTGTGTTTCAAATTTTAGT 515
GPCR3 : 420 CCTTCTGTTGGTGGTGGTCGTGACTGGAGGTTTTCTGCATTCTGTGTTTCAAATTGTTGT 479
NOVll: 516 TGTGTACAGTCTCCCTTTCTGTGGCCCCAATGTCATTTATCACTTTTTCTGTAACATATA 575
111 11 I I I II I I II I III I I I III II 1111 MM 1 I I I 111 Mill 1 II I II
GPCR3: 480 TGTATACAGTCTCGCTTTCTGTGGCCCCAATGTCATTGACTACTTTGTCTGTGACATGTA 539
NOVll: 576 CCCTTTATTGGACCTGGAATGCACTGACACCTACTTCGTAGGCCTCGCTGTGGTTTTCAA 635
III 11 llllll III I Mill I II I II II II Ml I II II I I I II II II II
GPCR3 : 540 CCCATTATTGGAACTGGTATGCACTGACACCTACTTTATTGGCCTTACTGTTTTTGTCAA 599
NOVll: 636 TGGTGGAGCAATCTGTATGGTCATCTTCACCCTTCTACTAATCTCCTATGGGGTCATCCT 695 lllllll I II I II II I I III II II I II II II II I II II I II II I II II 1 I III II I
GPCR3 : 600 TGGTGGAACAATCTGTATAGTCGTCTTCACCCTTCTACTAATCTCCTATGGAGTCATCCT 659
NOVll: 696 AAACTCCCTTAAAACTTATAGTCCGGAAGGGAGGCATAAAGCTCC-GTTTATCTGCAGCT 754 II Ml II I III I I I I III MM III I II III I I 111 II III 1 I III 11 II 11 I I
GPCR3 : 660 AAACTCCCTTAAAACTTACAGTCAAGAAGGGAGGCATAAAG-TCCTGTTTACCTGCAGCT 718
NOVll: 755 CCCACTTTATCATGGTTATCTTGTTTTTTGTTCCCTGTATTTTCTTATATGTTAGACCCG 814
Mill II Ml I II I I II II II II II II II II I 11 II I Ml II I II II I I
GPCR3 : 719 CCCACATTATCGTCTTTGCCCTCTTTTTTGTTCCCTGTATTTTCATGTATGTTAGACCTG 778
NOVll: 815 TTTCAAACTTTCCTATTGATAAATTCCTGACTGTGTTTTATTCAGTTATCACACCCAAGT 874
II I III II I III III I II II II II II II II 111 II I I I I I I II I MM I I II 11
GPCR3 : 779 TTTCAAACAT-CCTTTTGATAAATTCCTGACAGTGTTTTATACAGTTATCACACCCATGT 837
NOVll: 875 TGAATCCTTTTATATACATGTTGAGAAATTCAGAGATGAGAAATGCTATAGAAAATCTCT 934
II I II II II I 11 III III III III II I 11 MM I II llllll 11 I I
GPCR3 : 838 TGAATCCTTTAATATACACATTGAGAAATTCAGAGATGAGAAATTCTGTAGAAACACTCT 897
NOVll: 935 TGGGATACCAAAGT 948 (SEQ ID NO. 75)
II I I I I II 1 I GPCR3: 898 TGTG-TA—AAAGT 908 (SEQ ID NO. 76)
TABLE 12C.
NOVll: 1 MRQNNNITEFVLLGFSQYPDVQNALFVMFLLIYIVTMVGNLLIVVSIIASPFLGSPVYFF 60
I +1 I I I I I l + l I I +1 II + MM + I I I I I I I l + l I I I I I I++I I I I I I I l + l I I
ODOR1: 1 MGENNNITEFILLGLTQDPDGRKALFVIFFLIYIVTMMGNLLIVVTVIASPSLGSPMYFF 60
NOVll: 61 LACLSFIDAVYSTTISPVLIVDLLCDKKTISFPACMGQLFIEHLFGDTDVFLLVVMAYDR 120
II II +M++M III II III l + M II I III I I I I I I I I I 1+ +11 II II I
ODOR1 : 61 LASLSLLDALFSTAISPKLIADLLYDQKTISFRACMSQLFIEHLFGGVDIVILVAMAYDR 120
NOVll : 121 YVATCKPLRYLTIMNRQVCILLLWAVTGGFLHSVFQILVVYSLPFCGPNVIYHFFCNIY 180
III I I I I II I I I l + l I I 11+ I I I I I 11+ 11+ I l + l I I I I I I I I 11 I++
ODOR1 : 121 YVAICKPLHYLAIMNRRVCITLLIFAWTGGFTHSLIQIVFVYNLPFCGPNVIDHFICDMS 180
NOVll: 181 PLLDLECTDTYFVGLAVVFNGGAICMVIFTLLLISYGVILNSLKTYSPEGRHKAPFICSS 240
I II I I II 11 l + M 1+ III l + l I I II II II l + l I I I I I I III II 111
ODOR1 : 181 PLLVLACTDTYFIGLTVIANGGVNCIVIFTLLLGSYGIILRSLKTQSQEGRRKALSTCSS 240
NOVll: 241 HFIMVILFFVPCIFLYVRPVSNFPIDKFLTVFYSVITPKLNPFIYMLRNSEMRNAIENL 299
I ++I I I I I I I I I l + l ill +1 II I ++ II I III II I I I II+++ ++ I
ODOR1 : 241 HILVVILFFVPCIFMYARPVYNFPIDKCITVFYTIITPMLNPLIYTLRNSEIKSCMKKL 299
NOVll: (SEQ ID NO. 77)
ODOR1: (SEQ ID NO. 78)
Where | indicates identity and + indicates similarity.
NOV12 A NOVl 2 sequence according to the invention includes a nucleic acid sequence encoding a polypeptide related to the GPCR family of proteins. A NOV12 nucleic acid is found on human chromosome 11. A NOV12 nucleic acid and its encoded polypeptide include the sequences shown in Table 13A. The disclosed nucleic acid (SEQ LD NO.: 23) is 988 nucleotides in length and contains an open reading frame (ORF) that begins with an ATG initiation codon at nucleotides 23-25 and ends with a TAA stop codon at nucleotides 959-961. A putative untranslated region upstream from the initiation codon and downstream from the termination codon is underlined in Table 13 A, and the start and stop codons are in bold letters. The representative ORF encodes a 312 amino acid polypeptide (SEQ ID NO.: 24) with a predicted molecular weight of 35354.0 Da. PSORT analysis of a NOV12 polypeptide predicts a plasma membrane protein with a certainty of 0.6000. SIGNALP analysis suggests the presence of a signal peptide with the most likely cleavage site occuring between positions 38 and 39 in SEQ ID NO.: 24. TABLE 13A.
GTTGTCACAGCCGACAGAGGCAATGGATGAAGCCAATCACTCTGTGGTCTCTGAG TTTGTGTTCCTGGGACTCTCTGACTCGCGGAAGATCCAGCTCCTCCTCTTCCTCTTT TTCTC AGTGTTCTATGTATCAAGCCTGATGGGAAATCTCCTC ATTGTGCTAACTGT GACCTCTGACCCTCGTTTACAGTCCCCCATGTACTTCCTGCTGGCCAACCTTTCCAT CATCAATTTGGTATTTTGTTCCTCCACAGCTCCCAAGATGATTTATGACCTTTTCAG GAAGCACAAGACCATCTCTTTTGGGGGCTGTGTAGTTCAGATCTTCTTTATCCATG CAGTTGGGGGAACTGAGATGGTGCTGCTCATAGCCATGGCTTTTGACCGATATGT GGCCATATGTAAGCCTCTCCACTACCTGACCATCATGAACCCACAAAGGTGCATTT TGTTTTTAGTCATTTCCTGGATTATAGGTATTATTCACTCAGTGATTCAGTTGGCTT TTGTTGTAGACCTGCTGTTCTGTGGCCCTAATGAATTAGATAGTTTCTTTTGTGATC TTCCTCGATTTATCAAACTGGCTTGCATAGAGACCTACACATTGGGATTCATGGTT ACTGCCAATAGTGGATTTATTTCTCTGGCTTCTTTTTTAATTCTCATAATCTCTTAC ATCTTTATTTTGGTGACTGTTCAGAAAAAATCTTCAGGTGGTATATTCAAGGCTTT CTCTATGCTGTCAGCTCATGTCATTGTGGTGGTTTTGGTCTTTGGGCCATTAATCTT TTTCTATATTTTTCCATTTCCCACATCACATCTTGATAAATTCCTTGCCATCTTTGA TGCAGTTATCACTCCCGTTTTGAATCCAGTCATCTATACTTTTAGAAATAAAGAGA TGATGGTGGCAATGAGAAGACGATGCTCTCAGTTTGTGAATTACAGTAAAATCTTT TAAATATATTGAGAATATACAAAAAGGCAA (SEQ LD NO.: 23)
MDEANΉSVVSEFVFLGLSDSRKIQLLLFLFFSVFYVSSLMGNLLIVLTVTSDPRLQSPM YFLLANLSILNLVFCSSTAPKMIYDLFR-EAIKTISFGGCVVQIFFIHAVGGTEMVLLIAMA
FDRYVAICKPLHYLTLMNPQRCILFLVISWIIGIIHSVIQLAFVVDLLFCGPNELDSFFCD LPRFIKLACIETYTLGFMVTANSGFISLASFLILΠSYIFILVTVQKKSSGGIFKAFSMLSA LW VVLVFGPL--FFY--FPFPTSHLDKFLAIFDAVITPVLNPVIYTFR-NKEMMVAMRRRC
SQFVNYSKIF (SEQ LD NO.: 24)
The reverse complement (SEQ LD NO. 75) of aNOV12 nucleic acid sequence (SEQ ID NO. 23) has homology (73% identity) to a Homo sapiens GPCR mRNA (GPCR4;
GENBANK-LD: AL031259), as is shown in Table 13B. A NOV12 polypeptide has homology (48%o identity, 60% similarity) with a Mus musculus odorant receptor protein (ODOR2; SPTREMBL-ACC: BAA86125), as is shown in Table 13C. The global sequence homology (as defined by GAP alignment with the full length sequence of this protein) is 48 % amino acid identity and 57 % amino acid similarity. In addition, this protein contains a seven transmembrane receptor domain (rhodopsin family) (LPR000276) from amino acid residues 41 to 287 (as defined by Interpro).
TABLE 13B.
NOVl 2: 966 TAT-ATTTAAAAGATTTTACTGTAATTCACAAACTGAGAGCATCGTCTTCTCATTGCCAC 908
I I I I III I llll ll II III III I I II I 1111 III l lllll I
GPCR4 : 8393 TATCATTTATGAGATCTTCTTGTAAATCACTAGCTGTTTGCATACTCTCTTTATTGCTGC 8452
NOVl 2 : 907 CATCATCTCTTTATTTCTAAAAGTATAGATGACTGGATTCAAAACGGGAGTGATAACTGC 848
I II II I II II MM II II II II l
GPCR4 : 8453 CTTCATCTCCTTATTCCTGAATGTATAGACAACTGGATTCAGAAAAGGAGTGAGAACTGC 8512
NOVl 2: 847 ATCAΆAGATGGCAAGGAATTTATCAAGATGTGATGTGGGAAATGGAAAAATATAGAAAΆΆ 788
II II I I I I II II II II I I I II III I II Ml I Ml I I Ml
GPCR4 : 8513 ATCAΆAAATAGCCAGAAACTTGTCCATCTGTGAATTAGGGTGTGGCCATGTATACACAAA 8572
NOV12: 87 GATTAATGGCCCAΆAGACCAAAACCACCACAATGACATGAGCTGACAGCATAGAGAAAGC 728
II II I I III Ml II III II I I II II I I 11 II I I I I I I II
GPCR4 : 8573 CATGGGTGGACCAAΆGAΆCAAAAGGACCGCTGTGCTGTGAGCTGAAAGAGTGGAΆAGGGC 8632
NOVl 2 : 727 CTTGAATATACCACCTGAAGATTTTTTCTGAACAGTCACCAAAATAAAGATGTAAGAGAT 668 llll ll II II I II II II Mill II 1111 I I 1 I 1 ) I II 11 II I 11 I I
GPCR4 : J633 CTTGGATGAACCACCTGAGGAATGTTTCCAAACAGTAAACAGGATGAAGATGTAGGAGAT 8692
NOVl 2 : 667 TATGAGAATTAAAAAAGAAGCCAGAGAAATAAATCCACTATTGGCAGTAACCATGAATCC 608
II 11 II II MM I II I I I Mill I I I II II I I I I II III III
GPCR4 : 8693 TAGAAGTATGAAGAAAGTACCCACACAGATAAACCCACTGTTAACAGTGACCATGAACTG 8752
NOVl 2 : 607 CAATGTGTAGGTCTCTATGCAAGCCAGTTTGATAAATCGAGGAAGATCACAAAAGAAACT 548
I III I II II II II I II I I II I I II I I I I I 111 I I I I I I llll l l
GPCR4 : 8753 CAATCTGTAGGTGTCGGTACAGGCTAGTCTGAGAAGCTGAGGAAGGTCACAGTAGAAGCT 8812
NOVl 2: 547 ATCTAATTCATTAGGGCCACAGAACAGCAGGTCTACAACAAAAGCCAACTGAATCACTGA 488
II II II II III III II II II I I I II II II I I II I II I II II
GPCR : .813 GTCCAACACATTAGGGCCACAGAAGGGTAAATTAACAAGAAATGCCAGTTGGAACAGGGA 8872
NOV12 : 487 GTGAATAATACCTATAATCCAGGAAATGACTAAAAACAAAATGCACCTTTGTGGGTTCAT 428
II II I I II I I II II II II M ill II I I II I II II II II II GPCR4 : 8873 GTGACTGACACCAAGGGTCCAGGCAACAGCCAGAAATGAAΆGGCACATTCTTGGGCTCAT 8932
NOVl2: 427 GATGGTCAGGTAGTGGAGAGGCTTACATATGGCCACATATCGGTCAAAAGCCATGGCTAT 368
II III I I I I I II II II III I II III II II II II I I llllll II III III II I
GPCR4 : 8933 AATGGTCAGATAGTGGAGGGGCTTA-ATAGGGCCACATAACTGTCAAAGGCCATGGCTAT 8991
NOVl2: 367 GAGCAGCACCATCTCAGTTCCCCCAACTG-CATGGATAAAGAAGATCTGAACTACACAGC 309
GPCR4 : 8992 GAGCAGCACCATCTCCACACCACCAA-TGACGTGGATGAAGAAGATTTGAGCGATGCAGC 9050
NOVl2: 308 CCCCAAAAGAGATGGTCTTGTGCTTCCTGAAAAGGTCATAAATCATCTTGGGAGCTGTGG 249
1 I Ml I III I II I 11 I II III II 111 I 111 III MM III III
GPCR4 : 9051 CTCCAAAGGAGATGACTTTGCGCTTTCTGAACAGGTCATAAATCATCTTGGGAGAAGTGA 9110
NOVl2 : 248 AGGAACAAAATACCAAATTGATGATGGAAAGGTTGGCCAGCAGGAAGTACATGGGGGACT 189
I I I I I I I I I I I I I M l I I I I I I I I I I I I I I I I I I I I I I I I I I
GPCR4 : 9111 CAGAGCAGGCTCCTAAGTCAATGAAGGAGAGACTGGCCAGTAGAAAGTACATGGGGGAGT 9170
NOVl2: 188 GTAAACGAGGGTCAGAGGTCACAGTTAGCACAATGAGGAGATTTCCCATCAGGCTTGATA 129
GPCR4 : 9171 GTAAGTGAGGGTCAGTGGTCACAGAAAACACAATGAGGATGTTTCCAGTAATGCTTGCCA 9230
NOVl 2: 128 CATAGAACACTGAGAAAAAGAGGAAGAGGAGGAGCTGGATCTTCCGCGAGTCAGAGAGTC 69 llllll III III I 11 I I I I II I I III II I I Ml I 1 II II I 11 II I I I
GPCR4 : 9231 CATAGAGCACAGAGGAAAACACTAGGAGGAGGAGCTGGATCTCCCATGAATGAGTGAGTC 9290
NOVl2 ; 68 CCAGGAACACAAACTCAGAGACCACAGAGTGATTGGCTTCATCCATTGCCTCTGTCGGCT
I II I I I II II I II III I II II Mill II II II 1 I III II II 1 1 1 II
GPCR4 : 9291 CCAGAAACAAAAACTCAGATACCACTGAGTGATTCTCTCCATCCATTGGTCCAGCCAACT 9350
NOV12: 8 GTG 6 (SEQ ID NO. 79) I I
GPCR4: 9351 GGG 9353 (SEQ ID NO. 80)
TABLE 13C.
NOV12: 1 MDEANHSVVSEFVFLGLSDSRKIQLLLFLFFSVFYVSSLMGNLLIVLTVTSDPRLQSPMY 60 I I + I+II+IIII+I+ +++I I+ I+I I+ +I+II III+I+ III +111
ODOR2: 1 MGALNQTRVTEFIFLGLTDN VLEILFFVPFTVTYMLTLLGNFLIVVTIVFTPRLHNPMY 60
NOVl2 : 61 FLLANLSIINLVFCSSTAPKMIYDLFRKHKTISFGGCVVQIFFIHAVGGTEMVLLIAMAF 120
I l + l I I I++ I I I I 1+ I + I I I I I I + l + l l + l +I+ M 11 +
ODOR2 : 61 FFLSNLSFIDICHSSVTVPKMLEGLLLERKTISFDNCIAQLFFLHLFACSEIFLLTIMAY 120
NOVl2: 121 DRYVAICKPLHYLTIMNPQRCILFLVISWIIGIIHSVIQLAFVVDLLFCGPNELDSFFCD 180 l llll l l lll l +11 + 1+ + I+ I IM++I + I +1111 +11 + 111
ODOR2 : 121 DRYVAICIPLHYSNVMNMKVCVQLVFAL LGGTIHSLVQTFLTIRLPYCGPNIIDSYFCD 180 NOVl2: 181 LPRFIKLACIETYTLGFMVTANSGFISLASFLILIISYIFILVTVQKKSSGGIFKAFSML 240
+ 1 I I I II +11 I ++ +11 I 11 I 1 I 1+ II 11 +++II 1+ I II I
ODOR2 : 181 VPPVIKLACTDTYLTGILIVSNSGTISLVCFLALVTSYTVILFSLRKKSAEGRRKALSTC 240
NOVl2 : 241 SAHVIVWLVFGPLIFFYIFPFPTSHLDKFLAIFDAVITPVLNPVIYTFRNKEMMVAM— 298
I I I + 1 1 M M I I I I + + M +++ I I + M + I M + I M M + I + I I
ODOR2 : 241 SAHFMVVTLFFGPCIFLYTRPDSSFSIDKVVSVFYTVVTPLLNPLIYTLRNEEVKTAMKH 300
NOVl2: 917 -RRRCS 303 (SEQ ID NO. 81) II I I ODOR2: 301 LRQRRICS 308 (SEQ ID NO. 82)
Where | indicates identity and + indicates similarity.
Novel SNP variants of NOV12 and their corresponding polypeptides are shown in Table 13D. The method of identifying and confirming novel SNPs is described in Example 3.
Table 13D. 1(a). Nucleotide sequence of variant 1 (variant 1 at position 368).
1
TTATATTTAAAAGATTTTACTGTAATTCACAAACTGAGAGCATCGTCTTCTCATTGCCACCATCATCTCTTTATTTC
TAA 8i
AAGTATAGATGACTGGATTCAAAACGGGAGTGATAACTGCATCAAAGATGGCAAGGAATTTATCAAGATGTGATGTG
GGA 161
-^TGGAAAAATATAGAAAAAGATTAATGGCCCAAAGACCAAAACCACCACAATGACATGAGCTGACAGCATAGAGAA AGC 241
CTTGAATATACCACCTGAAGATTTTTTCTGAACAGTCACCA-a-AATAAAGATGT-^GAGATTATGAG- TTA-\AAAAG AAG 321 CCAGAGAAATAAATCCACTATTGGCAGTAACCATGAATCCCAATGTGCAGGTCTCTATGCAAGCCAGTTTGATAAAT
CGA 401
GGAAGATCACAAAAGAAACTATCTAATTCATTAGGGCCACAGAACAGCAGGTCTACAACAAAAGCCAACTGAATCAC
TGA 481 GTGAATAATACCTATAATCCAGGAAATGACTAAAAACAAAATGCACCTTTGTGGGTTCATGATGGTCAGGTAGTGGA
GAG 561 GCTTACATATGGCCACATATCGGTCAAAAGCCATGGCTATGAGCAGCACCATCTCAGTTCCCCCAACTGCATGGATA AAG 641
AAGATCTGAACTACACAGCCCCCAAAAGAGATGGTCTTGTGCTTCCTGAAAAGGTCATAAATCATCTTGGGAGCTGT GGA 721 GGAACAAAATACCAAATTGATGATGGAAAGGTTGGCCAGCAGGAAGTACATGGGGGACTGTAAACGAGGGTCAGAGG
TCA 801
CAGTTAGCACAATGAGGAGATTTCCCATCAGGCTTGACACATAGAACACTGAGAAAAAGAGGAAGAGGAGGAGCTGG ATC 881 TTCCGCGAGTCAGAGAGTCCCAGGAACACAAACTCAGAGACCACAGAGTGATTGGCTTCATCCATTGCCTAAGG (SEQ ID NO. : 83)
1(b). Protein Sequence of variant 1.
1 MDEANHSWSEFVFLGLSDSRK1QLLLFLFFSVFYVSSLMGNLLIVLTVTSDPRLQSPMYFLLANLSIINLVFCSST
APK 81
MIYDLFRKHKTISFGGCWQIFFIHAVGGTE VLLIAMAFDRYVAICKPLHYLTIMNPQRCILFLVISWIIGIIHSV IQL 161
AFWDLLFCGPNELDSFFCDLPRFIKLACIETCTLGFMVTANSGFISLASFLILIISYIFILVTVQKKSSGGIFKAF SML
241 SAHVIVWLVFGPLIFFYIFPFPTSHLDKFLAIFDAVITPVLNPVIYTFRNKEMMVAMRRRCSQFVNYSKIF (SEQ ID NO. : 84)
1(c) Effect of variant 1 on amino acid residue
Tyr to Cys at position 193.
2(a) Nucleotide sequence of variant 2 (variant atpostion 600).
1
TTATATTTAAAAGATTTTACTGTAATTCACAAACTGAGAGCATCGTCTTCTCATTGCCACCATCATCTCTTTATTTC
TAA
81 AAGTATAGATGACTGGATTCAAAACGGGAGTGATAACTGCATCAAAGATGGCAAGGAATTTATCAAGATGTGATGTG
GGA
161
AATGGAAAAATATAGA-iAAAGATTAATGGCCCAAAGACCAAAACCACCACAATGACATGAGCTGACAGCATAGAGAA AGC 241
CTTGAATATACCACCTGAAGATTTTTTCTGAACAGTCACCAAAATAAAGATGTAAGAGATTATGAGAATTAAAAAAG AAG 321
CCAGAGAAATAAATCCACTATTGGCAGTAACCATGAATCCCAATGTGTAGGTCTCTATGCAAGCCAGTTTGATAAAT CGA 401
GGAAGATCACAAAAGAAACTATCTAATTCATTAGGGCCACAGAACAGCAGGTCTACAACAAAAGCCAACTGAATCAC TGA 481 GTGAATAATACCTATAATCCAGGAAATGACTAAAAACAAAATGCACCTTTGTGGGTTCATGATGGTCAGGTAGTGGA
GAG 561
GCTTACATATGGCCACATATCGGTCAAAAGCCATGGCTACGAGCAGCACCATCTCAGTTCCCCCAACTGCATGGATA AAG 641
AAGATCTGAACTACACAGCCCCCAAAAGAGATGGTCTTGTGCTTCCTGAAAAGGTCATAAATCATCTTGGGAGCTGT GGA 721
GGAACAAAATACCAAATTGATGATGGAAAGGTTGGCCAGCAGGAAGTACATGGGGGACTGTAAACGAGGGTCAGAGG TCA 801
CAGTTAGCACAATGAGGAGATTTCCCATCAGGCTTGACACATAGAACACTGAGAAAAAGAGGAAGAGGAGGAGCTGG ATC
881 TTCCGCGAGTCAGAGAGTCCCAGGAACACAAACTCAGAGACCACAGAGTGATTGGCTTCATCCATTGCCTAAGG (SEQ ID NO. : 85)
2(b) Protein Sequence of variant 2.
1
MDEANHSWSEFVFLGLSDSRKIQLLLFLFFSVFYVSSLMGNLLIVLTVTSDPRLQSPMYFLLANLSIINLVFCSST APK 81
MIYDLFRKHKTISFGGCVVQIFFIHAVGGTEMVLLIAMAFDRYVAICKPLHYLTIMNPQRCILFLVIS IIGIIHSV IQL 161 AFWDLLFCGPNELDSFFCDLPRFIKLACIETYTLGFMVTANSGFISLASFLILIISYIFILVTVQKKSSGGIFKAF
SML
241 SAHVIVWLVFGPLIFFYIFPFPTSHLDKFLAIFDAVITPVLNPVIYTFRNKEMMVAMRRRCSQFVNYSKIF (SEQ
ID NO . : 86) 2(c) Effect of variant 2 on amino acid residue
Tyr to Cys at position 193 .
3(a) Nucleotide sequence of variant 3.
At position 130, A > G. 3(b) Protein Sequence of variant 3.
No change from NOV12 protein sequence.
NOVl 2 expression was analyzed by TaqMan analysis. The expression of NOV12 in indicates highest levels in skeletal muscle and lower levels in the adipose tissue, as shown in Example 4 at Table 20B (Panel 1.2). The expression in skeletal muscle could indicate that this GPCR may be involved in energy metabolism and regulation of either carbohydrate or lipid as energy sources. It therefore could be a potential small molecule target in the treatment of diabetes and/or obesity.
Polypeptides encoded by NOV1-NOV12 nucleic acids were analyzed by CLUSTALW analysis, as is shown in Table 21. TABLE 21.
NOV9 MDTG-NKTLPQDFLLLGFPGSQTLQLSLFMLFLVMYILTVSGNVAILMLVSTSHQLH
NOV8 MDTG-NKTLPQDFLLLGFPGSQTLQLSLFMLFLVMYILTVSGNVAILMLVSTSHQLH rOR -MAWSTGQNLSTPGPFILLGFPGPRSMRIGLFLLFLVMYLLTVVGNLAIISLVGAHRCLQ NOV12 MDEANHSVVSEFVFLGLSDSRKIQLLLFLFFSVFYVSSL GNLLIVLTVTSDPRLQ
NOVll MRQNNN—ITEFVLLGFSQYPDVQNALFVMFLLIYIVTMVGNLLIVVSIIASPFLG
NOV6
NOV10 MERPQSDFNQTEVAEFFLMGFSNSWDIQIVHAALFFLVYLAAVIGNLLIIILTTLDVHLQ NOV7 MT NQTQMMEFLLVRFTEN VLLRLHALLFSLIYLTAVLMNLVIILLMILDHRLH NOV5 GLEYAHIWISIPICSMYLIAILGNCTILFIIKTEPSLH
NOV3 — SI1NTSYVEITTFFLVG PGLEYAHIWISIPICSMYLIA1LGNGTILFIIKTEPSLH NOV4 —MSIINTSYVEITTFFLVGMPGLEYAHI ISIPICSMYLIAILGNGTILFIIKTEPSLH NOV2 —MSIINTSYVEITTFFLVGMPGLEYAHIWISIPICSMYLIAILGNGTILFIIKTEPSLH NOVl —MFLPNNTQFHPSSFLLLGIPGLETLHI IGFPFCAVYIIALIGRFTILLVIKTDSSLY
NOV9 TP YFFLSNLSFLEIWYTTAAVPKALAILLGRSQTISFTSCLLQMYFVFSLGCTEYFLLA NOV8 TPMYFFLSNLSFLEI YTTAAVPKALAILLGRSQTISFTSCLLQMYFVFSLGCTEYFLLA rOR TPMYFFLCNLSFLEIWFTTACVPKTLATFAPRGGVISLAGCATQMYFVFSLGCTEYFLLA NOV12 SPMYFLLANLSIINLVFCSSTAPKMIYDLFRKHKTISFGGCWQIFFIHAVGGTEMVLLI NOVll SPVYFFLACLSFIDAVYSTTISPVLIVDLLCDKKTISFPACMGQLFIEHLFGDTDVFLLV NOV6 —MYVFLLTLAVVDI1CTTSIIPKMLGTMLTSENTISYAGCMSQLFLFT SLGAEMVLFT NOV10 TPMYFFLRNLSFLDFCYISVTIPKSIVSSLTHDTSISFFGCALQAFFFMDLATTEVAILT NOV7 AMYFFLRHLSFLDLCLISATVPKSILNSVASTDSISFLGCVLQLFLWLLAGSEIGILT NOV5 EPMYYFLSMLAMSDLGLSLSSLPTVLSIFLFNAPEISSNACFAQEFFIHGFSVLESSVLL NOV3 EPMYYFLS LAMSDLGLSLSSLPTVLSIFLFNAPEISSNACFAQEFFIHGFSVLESSVLL
NOV4 GPMYYFLSMLAMSDLGLSLSSLPTVLSIFLFNAPETSSNACFAQEFFIHGFSVLESSVLL
NOV2 GPMYYFLS LAMSDLGLSLSSLPTVLSIFLFNAPETSSSACFAQEFFIHGFSVLESSVLL
NOVl QPMFYFLAMLATIDLGLSTATIPKMLGIF FSLREIICDACLIQMFFIHNFTGMESAALV
.. .* *. . * . * * .. . .
NOV9 AMAYDRCLAICYPLHYGAIMSSLLSAQLALGS VCGFVAIAVPTALISGLSFCGPRAINH NOV8 AAYDRCLAICYPLHYGAIMSSLLSAQLALGS VCGFVAIAVPTALISGLSFCGPRAINH rOR VMAYDRYLAICLPLRYGGI TPGLAMRLALGSWLCGFSAITVPATLIARLSFCGSRVINH NOV12 AMAFDRYVAICKPLHYLTIMNPQRCILFLVIS IIGIIHSVIQLAFVVDLLFCGPNELDS NOVll VMAYDRYVATCKPLRYLTI NRQVCILLLVVAVTGGFLHSVFQILWYSLPFCGPNVIYH NOV6 TMAYDRYVAICFPLHYSTVMNHHMCVALLSMV AI VTNS VHTA IMRLTFCGPNTIDH NOV10 V SYDRYt^ICRPLHYEVIINQGVCLRfctMAMSWLSGVICGFMHVIATFSLPFCGRNRIRQ NOV7 A SYDRYAAICCPLHCEAVMSRGLCVQLMALS LNRGALGLLYTAGTFSLNFYGSDELHQ NOV5 I SFDRFLAIHNPLRYTSILTTVRVAQIGIVFSFKSMLLVLPFPFTLRNLRYCKKNQLSH NOV3 IMSFDRFLAIHNPLRYTSILTTVRVAQIGIVFSFKSMLLVLPFPFTLRNLRYCKKNQLSH NOV4 IMSFDRFLAIHNPLRYTSILTTVRVAQIGIVFSFKSMLLVLPFPFTLRSLRYCKKNQLSH NOV2 IMSFDRFLAIHNPLRYTSILTTVRVAQIGIVFSFKSMLLVLPFPFTLRSLRYCKKNQLSH NOVl G AYDHFVAICNPLRYSIILTKKAVSVIGLGVLVRSFMSVIPFVFLILRLPFCGDHVIPH
NOV9 FFCDIAPWIALACTNTQAVELVAFVIAWVILSSCLITFVSYVYIISTILRIPSASGRSK NOV8 FFCDIAPWIALACTNTQAVELVAFVIAVWILSSCLITFVSYVYIISTILRIPSASGRSK rOR FFCDISPWIVLSCTDTQWELVSFGIAFCVILGSCGITLVSYAYIITTIIKIPSARGRHR NOV12 FFCDLPRFIKLACIETYTLGFMVTANSGFISLASFLILIISYIF1LVTVQKK-SSGGIFK NOVll FFCNIYPLLDLECTDTYFVGLAVVFNGGAICMVIFTLLLISYGVILNSLKTY-SPEGRHK NOV6 FFCEIPPLLALSCSPVRINEVMVYVAD1TLAIGDFILTCISYGF1IVAILRIRTVEGKRK NOV10 FFCNIPQLLSLLDPKVITIEIGV VFGTSLVIISFVVITLSYMYIFSVIMRIPSKEGRSK
NOV7 FFCDVPALLKLTCSKEHAIISVSVAIGVCYAFSCLVCIWSYVYIFSAVLRISQRQRQSK
NOV5 SYCLHQDVMKLACSDNR-IDVIYGFFGALCLMVDFILIAVSYTLILKTVLGIASKKEQLK
NOV3 SYCLHQDVMKLACSDNR-IDVIYGFFGALCLMVDFILIAVSYTLILKTVLGIASKKEQLK NOV4 SYCLHQDVMKLACSDNR-IDVIYGFFGALCLMVDFILIAVSYTLILKTVPGIASKKEQLK
NOV2 SYCLHQDVMKLACSDNR-IDVIYGFFGALCLMVDFILIAVSYTLILKTVPGIASKKEELK
NOVl TNCEHMGLAHLSCSSIK-INIIYGLGAISILVFDIIAIALSYVQILHAVFHLPSCKARLK * * . :** *: : : NOV9 AFSTCSSHLTWLIWYGSTVFLHVRT—SIKDALDLIKAVHVLNTWTPVLNPFIYTLRN
NOV8 AFSTCSSHLTWLIWYGSTVFLHVRT—SIKDALDLIKAVHVLNTVVTPVLNPFIYTLRN
ROR AFSTCSSHLTWLIWYGSTIFLHVRT—SVESSLDLTKAITVLNTIVTPVLNPFIYTLRN
NOV12 AFS LSAHVIWVLVFGPLIFFYIFP—FP—TSHLDKFLAIFDAVITPVLNPV1YTFRN
NOVll APFICSSHFIMVILFFVPCIFLYVRP—VS—NFPIDKFLTVFYSVITPKLNPFIYMLRN NOV6 AFSTCSSHLTWTLYYSPVIYTYIRP—AΞSYTFERDKWAALYTLVTPTLNPMVYSFQN
NOV10 TFSTCIPHLWVTLFMISGSIAYVKP—ISNSPPVLDVFLSAFYTWPPTLNPVIYSLRN
NOV7 AFSNCVPHLIWTVFLVTGAVAYLKP—GSDAPSILDLLVSVFYSVAPPTLNPVIYCLKN
NOV5 ALNTCVSHICAVIIFYLPIINLAVVHRFARHVSPLINVLMANVLLLVPPLMNPIVYCVKT
NOV3 ALNTCVSHICAVIIFYLPIINLAWHRFARHVSPLINVLMANVLLLVPPLTNPIVYCVKT NOV4 ALNTCVSHICAVIIFYLPIINLAVVHRFARHVSPLINVLMANVLLLVPPLMKPIVYCVKT
NOV2 ALNTCVSHICAVIIFYLPIINLAWHRFAGHVSPLINVLMANVLLLVPPLMKPIVYCVKT
NOVl SLSTCGSHVCVILAFYTPALFSFVTHRFGQNVPRYIHILLANLYWVPPMLNPVIYGVRT
NOV9 KEVRETLL KKWKGK (SEQ ID NO. : 18)
NOV8 KEVRETLL KKWKGK (SEQ ID NO. : : 16) rOR KDVKEALR RTVKGK (SEQ ID NO. : : 70)
NOV12 KEMMVAMR RRCSQFVNYSKIF (SEQ ID NO. : : 24)
NOVll SEMRNAIENLLGYQSGKTGFRCSKLN (SEQ ID NO. : : 22)
NOV6 REMQAGIR KVFAFLKH- (SEQ ID NO. : : 12)
NOV10 RD KAALR RQCGP (SEQ ID NO. : : 20)
NOV7 KDIKSALSKVLWNVRSSGVMKDD (SEQ ID NO. : ; 14)
NOV5 KQIRVRVV AKLCQRKI- (SEQ ID NO. : : 10)
NOV3 KQIRVRVV AKLCQRKI- (SEQ ID NO. : : 6)
NOV4 KQIRVRVV AKLCQWKI- (SEQ ID NO. : : 8)
NOV2 KQIRVRVV AKLCQWKI- (SEQ ID NO. ; : 4)
NOV1 KQIYVCVK NIFLQK (SEQ ID NO. : : 2)
Where * indicates identity, : indicates strong identity and . indicates weak identity. rOR indicates an olfactory-like receptor protein from Rattus norvegicus (SWISSPROT-ACC: P23267).
NOVl through NOV12 represent new members of the GPCR family. NOVl through NOV12 can be used as markers for human chromosome 11. NOVl through NOV12 are useful in determining changes in expression of genes contained within the GPCR protein family. NOVl through NOV12 satisfy a need in the art by providing new diagnostic or therapeutic compositions useful in the treatment of disorders associated with alterations in the expression of members of the GPCR-associated protein families. Therefore, NOVl through NOV12, nucleic acids and proteins, and other compositions of the present invention identified here may be useful in potential therapeutic applications implicated in (but not limited to) various pathologies and disorders as indicated below. The potential therapeutic applications for this invention include, but are not limited to: protein therapeutic, small molecule drug target, antibody target (therapeutic, diagnostic, drug targeting/cytotoxic antibody), diagnostic and/or prognostic marker, gene therapy (gene delivery/gene ablation), research tools, tissue regeneration in vivo and in vitro of all tissues and cell types composing (but not limited to) those defined here.
The nucleic acids and proteins of the invention are useful in potential therapeutic applications, including the treatment of infections such as bacterial, fungal, protozoal and viral infections (particularly infections caused by HIV-1 or HLV-2), pain, cancer (including but not limited to neoplasm, adenocarcinoma, lymphoma, prostate cancer, and uterine cancer), anorexia, bulimia, asthma, Parkinson's disease, acute heart failure, hypotension, hypertension, urinary retention, osteoporosis, Crohn's disease, multiple sclerosis, Albright hereditary ostoeodystrophy, angina pectoris, myocardial infarction, ulcers, asthma, allergies, benign prostatic hypertrophy, and psychotic and neurological disorders, including anxiety, schizophrenia, manic depression, delirium, dementia, severe mental retardation and dyskinesias, such as Huntington's disease or Gilles de la Tourette syndrome, and/or other pathologies and disorders. The polypeptides can be used as immunogens to produce antibodies specific for the invention, and as vaccines. They can also be used to screen for potential agonist and antagonist compounds. For example, a cDNA encoding the GPCR-like protein may be useful in gene therapy, and the GPCR-like protein may be useful when administered to a subject in need thereof. By way of nonlimiting example, the compositions of the present invention will have efficacy for treatment of patients suffering from bacterial, fungal, protozoal and viral infections (particularly infections caused by HLV-1 or HIV-2), pain, cancer (including but not limited to neoplasm, adenocarcinoma, lymphoma, prostate cancer, and uterine cancer), anorexia, bulimia, asthma, Parkinson's disease, acute heart failure, hypotension, hypertension, urinary retention, osteoporosis, Crohn's disease, multiple sclerosis, Albright hereditary ostoeodystrophy, angina pectoris, myocardial infarction, ulcers, asthma, allergies, benign prostatic hypertrophy, and psychotic and neurological disorders, including anxiety, schizophrenia, manic depression, delirium, dementia, severe mental retardation and dyskinesias, such as Huntington's disease or Gilles de la Tourette syndrome, and/or other pathologies and disorders. The novel nucleic acid encoding GPCR-like protein, and the GPCR-like protein of the invention, or fragments thereof, may further be useful in diagnostic applications, wherein the presence or amount of the nucleic acid or the protein are to be assessed. These materials are further useful in the generation of antibodies that bind immunospecifically to the novel substances of the invention for use in therapeutic or diagnostic methods. NOVX Nucleic Acids
The nucleic acids of the invention include those that encode a NOVX polypeptide or protein. As used herein, the terms "polypeptide" and "protein" are interchangeable.
In some embodiments, a NOVX nucleic acid encodes a mature NOVX polypeptide. As used herein, a "mature" form of a polypeptide or protein relates to the product of a naturally occurring polypeptide or precursor form or proprotein. The naturally occurring polypeptide, precursor or proprotein includes, by way of nonlimiting example, the full-length gene product, encoded by the corresponding gene. Alternatively, it may be defined as the polypeptide, precursor or proprotein encoded by an open reading frame described herein. The product "mature" form arises, again by way of nonlimiting example, as a result of one or more naturally occurring processing steps that may take place within the cell in which the gene product arises. Examples of such processing steps leading to a "mature" form of a polypeptide or protein include the cleavage of the N-teπninal methionine residue encoded by the initiation codon of an open reading frame, or the proteolytic cleavage of a signal peptide or leader sequence. Thus a mature form arising from a precursor polypeptide or protein that has residues 1 to N, where residue 1 is the N-terminal methionine, would have residues 2 through N remaining after removal of the N-terminal methionine. Alternatively, a mature form arising from a precursor polypeptide or protein having residues 1 to N, in which an N-terminal signal sequence from residue 1 to residue M is cleaved, would have the residues from residue M+l to residue N remaining. Further as used herein, a "mature" form of a polypeptide or protein may arise from a step of post-translational modification other than a proteolytic cleavage event. Such additional processes include, by way of non-limiting example, glycosylation, myristoylation or phosphorylation. In general, a mature polypeptide or protein may result from the operation of only one of these processes, or a combination of any of them. Among the NOVX nucleic acids is the nucleic acid whose sequence is provided in
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or a fragment thereof. Additionally, the invention includes mutant or variant nucleic acids of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or a fragment thereof, any of whose bases may be changed from the corresponding bases shown in SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, while still encoding a protein that maintains at least one of its NOVX-like activities and physiological functions. The invention further includes the complement of the nucleic acid sequence of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, including fragments, derivatives, analogs and homologs thereof. The invention additionally includes nucleic acids or nucleic acid fragments, or complements thereto, whose structures include chemical modifications.
One aspect of the invention pertains to isolated nucleic acid molecules that encode NOVX proteins or biologically active portions thereof. Also included are nucleic acid fragments sufficient for use as hybridization probes to identify NOVX-encoding nucleic acids (e.g., NOVX mRNA) and fragments for use as polymerase chain reaction (PCR) primers for the amplification or mutation of NOVX nucleic acid molecules. As used herein, the term "nucleic acid molecule" is intended to include DNA molecules (e.g., cDNA or genomic DNA), RNA molecules (e.g., mRNA), analogs of the DNA or RNA generated using nucleotide analogs, and derivatives, fragments and homologs thereof. The nucleic acid molecule can be single-stranded or double-stranded, but preferably is double-stranded DNA.
"Probes" refer to nucleic acid sequences of variable length, preferably between at least about 10 nucleotides (nt), 100 nt, or as many as about, e.g., 6,000 nt, depending on use. Probes are used in the detection of identical, similar, or complementary nucleic acid sequences. Longer length probes are usually obtained from a natural or recombinant source, are highly specific and much slower to hybridize than oligomers. Probes may be single- or double-stranded and designed to have specificity in PCR, membrane-based hybridization technologies, or ELISA-like technologies.
An "isolated" nucleic acid molecule is one that is separated from other nucleic acid molecules that are present in the natural source of the nucleic acid. Examples of isolated nucleic acid molecules include, but are not limited to, recombinant DNA molecules contained in a vector, recombinant DNA molecules maintained in a heterologous host cell, partially or substantially purified nucleic acid molecules, and synthetic DNA or RNA molecules. Preferably, an "isolated" nucleic acid is free of sequences which naturally flank the nucleic acid (i.e., sequences located at the 5' and 3' ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. For example, in various embodiments, the isolated NOVX nucleic acid molecule can contain less than about 50 kb, 25 kb, 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb or 0.1 kb of nucleotide sequences which naturally flank the nucleic acid molecule in genomic DNA of the cell from which the nucleic acid is derived. Moreover, an "isolated" nucleic acid molecule, such as a cDNA molecule, can be substantially free of other cellular material or culture medium when produced by recombinant techniques, or of chemical precursors or other chemicals when chemically synthesized. A nucleic acid molecule of the present invention, e.g., a nucleic acid molecule having the nucleotide sequence of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or a complement of any of this nucleotide sequence, can be isolated using standard molecular biology techniques and the sequence information provided herein. Using all or a portion of the nucleic acid sequence of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, as a hybridization probe, NOVX nucleic acid sequences can be isolated using standard hybridization and cloning techniques (e.g., as described in Sambrook et αl, eds., MOLECULAR CLONING: A LABORATORY MANUAL 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989; and Ausubel, et αl, eds., CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, New York, NY, 1993.)
A nucleic acid of the invention can be amplified using cDNA, mRNA or alternatively, genomic DNA, as a template and appropriate oligonucleotide primers according to standard PCR amplification techniques. The nucleic acid so amplified can be cloned into an appropriate vector and characterized by DNA sequence analysis. Furthermore, oligonucleotides corresponding to NOVX nucleotide sequences can be prepared by standard synthetic techniques, e.g., using an automated DNA synthesizer.
As used herein, the term "oligonucleotide" refers to a series of linked nucleotide residues, which oligonucleotide has a sufficient number of nucleotide bases to be used in a PCR reaction. A short oligonucleotide sequence may be based on, or designed from, a genomic or cDNA sequence and is used to amplify, confirm, or reveal the presence of an identical, similar or complementary DNA or RNA in a particular cell or tissue. Oligonucleotides comprise portions of a nucleic acid sequence having about 10 nt, 50 nt, or 100 nt in length, preferably about 15 nt to 30 nt in length. In one embodiment, an oligonucleotide comprising a nucleic acid molecule less than 100 nt in length would further comprise at lease 6 contiguous nucleotides of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or a complement thereof. Oligonucleotides may be chemically synthesized and may be used as probes.
In another embodiment, an isolated nucleic acid molecule of the invention comprises a nucleic acid molecule that is a complement of the nucleotide sequence shown in SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or a portion of this nucleotide sequence. A nucleic acid molecule that is complementary to the nucleotide sequence shown in SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 is one that is sufficiently complementary to the nucleotide sequence shown in SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 that it can hydrogen bond with little or no mismatches to the nucleotide sequence shown in SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, thereby forming a stable duplex.
As used herein, the term "complementary" refers to Watson-Crick or Hoogsteen base pairing between nucleotide units of a nucleic acid molecule, and the term "binding" means the physical or chemical interaction between two polypeptides or compounds or associated polypeptides or compounds or combinations thereof. Binding includes ionic, non-ionic, Von der Waals, hydrophobic interactions, etc. A physical interaction can be either direct or indirect. Indirect interactions may be through or due to the effects of another polypeptide or compound. Direct binding refers to interactions that do not take place through, or due to, the effect of another polypeptide or compound, but instead are without other substantial chemical intermediates.
Moreover, the nucleic acid molecule of the invention can comprise only a portion of the nucleic acid sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, e.g., a fragment that can be used as a probe or primer, or a fragment encoding a biologically active portion of NOVX. Fragments provided herein are defined as sequences of at least 6
(contiguous) nucleic acids or at least 4 (contiguous) amino acids, a length sufficient to allow for specific hybridization in the case of nucleic acids or for specific recognition of an epitope in the case of amino acids, respectively, and are at most some portion less than a full length sequence. Fragments may be derived from any contiguous portion of a nucleic acid or amino acid sequence of choice. Derivatives are nucleic acid sequences or amino acid sequences formed from the native compounds either directly or by modification or partial substitution. Analogs are nucleic acid sequences or amino acid sequences that have a structure similar to, but not identical to, the native compound but differs from it in respect to certain components or side chains. Analogs may be synthetic or from a different evolutionary origin and may have a similar or opposite metabolic activity compared to wild type.
Derivatives and analogs may be full length or other than full length, if the derivative or analog contains a modified nucleic acid or amino acid, as described below. Derivatives or analogs of the nucleic acids or proteins of the invention include, but are not limited to, molecules comprising regions that are substantially homologous to the nucleic acids or proteins of the invention, in various embodiments, by at least about 70%, 80%, 85%, 90%, 95%o, 98%, or even 99% identity (with a preferred identity of 80-99%) over a nucleic acid or amino acid sequence of identical size or when compared to an aligned sequence in which the alignment is done by a computer homology program known in the art, or whose encoding nucleic acid is capable of hybridizing to the complement of a sequence encoding the aforementioned proteins under stringent, moderately stringent, or low stringent conditions. See e.g. Ausubel, et al, CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, New York, NY, 1993, and below. An exemplary program is the Gap program (Wisconsin Sequence Analysis Package, Version 8 for UNIX, Genetics Computer Group, University
Research Park, Madison, WI) using the default settings, which uses the algorithm of Smith and Waterman (Adv. Appl. Math., 1981, 2: 482-489, which is incorporated herein by reference in its entirety).
A "homologous nucleic acid sequence" or "homologous amino acid sequence," or variations thereof, refer to sequences characterized by a homology at the nucleotide level or amino acid level as discussed above. Homologous nucleotide sequences encode those sequences coding for isoforms of a NOVX polypeptide. Isoforms can be expressed in different tissues of the same organism as a result of, for example, alternative splicing of RNA. Alternatively, isoforms can be encoded by different genes. In the present invention, homologous nucleotide sequences include nucleotide sequences encoding for a NOVX polypeptide of species other than humans, including, but not limited to, mammals, and thus can include, e.g., mouse, rat, rabbit, dog, cat cow, horse, and other organisms. Homologous nucleotide sequences also include, but are not limited to, naturally occurring allelic variations and mutations of the nucleotide sequences set forth herein. A homologous nucleotide sequence does not, however, include the nucleotide sequence encoding huma NOVX protein. Homologous nucleic acid sequences include those nucleic acid sequences that encode conservative amino acid substitutions (see below) SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24, as well as a polypeptide having NOVX activity. Biological activities of the NOVX proteins are described below. A homologous amino acid sequence does not encode the amino acid sequence of a huma NOVX polypeptide.
The nucleotide sequence determined from the cloning of the huma NOVX gene allows for the generation of probes and primers designed for use in identifying and/or cloning NOVX homologues in other cell types, e.g., from other tissues, as well as NOVX homologues from other mammals. The probe/primer typically comprises a substantially purified oligonucleotide. The oligonucleotide typically comprises a region of nucleotide sequence that hybridizes under stringent conditions to at least about 12, 25, 50, 100, 150, 200, 250, 300, 350 or 400 or more consecutive sense strand nucleotide sequence of SEQ ED NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23; or an anti-sense strand nucleotide sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23; or of anaturally occurring mutant of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23.
Probes based on the human NOVX nucleotide sequence can be used to detect transcripts or genomic sequences encoding the same or homologous proteins. In various embodiments, the probe further comprises a label group attached thereto, e.g., the label group can be a radioisotope, a fluorescent compound, an enzyme, or an enzyme co-factor. Such probes can be used as a part of a diagnostic test kit for identifying cells or tissue which misexpress a NOVX protein, such as by measuring a level of a NOVX-encoding nucleic acid in a sample of cells from a subject e.g., detecting NOVX mRNA levels or determining whether a genomic NOVX gene has been mutated or deleted.
A "polypeptide having a biologically active portion of NOVX" refers to polypeptides exhibiting activity similar, but not necessarily identical to, an activity of a polypeptide of the present invention, including mature forms, as measured in a particular biological assay, with or without dose dependency. A nucleic acid fragment encoding a "biologically active portion of NOVX" can be prepared by isolating a portion of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 that encodes a polypeptide having a NOVX biological activity (biological activities of the NOVX proteins are described below), expressing the encoded portion of NOVX protein (e.g., by recombinant expression in vitro) and assessing the activity of the encoded portion of NOVX. For example, a nucleic acid fragment encoding a biologically active portion of NOVX can optionally include an ATP -binding domain. In another embodiment, a nucleic acid fragment encoding a biologically active portion of NOVX includes one or more regions.
NOVX Variants
The invention further encompasses nucleic acid molecules that differ from the nucleotide sequences shown in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 due to the degeneracy of the genetic code. These nucleic acids thus encode the same NOVX protein as that encoded by the nucleotide sequence shown in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 e.g., the polypeptide of SEQ D NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24. In another embodiment, an isolated nucleic acid molecule of the invention has a nucleotide sequence encoding a protein having an amino acid sequence shown SEQ ED NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24.
In addition to the huma NOVX nucleotide sequence shown in SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, it will be appreciated by those skilled in the art that DNA sequence polymorphisms that lead to changes in the amino acid sequences of NOVX may exist within a population (e.g., the human population). Such genetic polymorphism in the NOVX gene may exist among individuals within a population due to natural allelic variation. As used herein, the terms "gene" and "recombinant gene" refer to nucleic acid molecules comprising an open reading frame encoding a NOVX protein, preferably a mammalia NOVX protein. Such natural allelic variations can typically result in 1-5% variance in the nucleotide sequence of the NOVX gene. Any and all such nucleotide variations and resulting amino acid polymorphisms in NOVX that are the result of natural allelic variation and that do not alter the functional activity of NOVX are intended to be within the scope of the invention. Moreover, nucleic acid molecules encoding NOVX proteins from other species, and thus that have a nucleotide sequence that differs from the human sequence of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 are intended to be within the scope of the invention. Nucleic acid molecules corresponding to natural allelic variants and homologues of the NOVX cDNAs of the invention can be isolated based on their homology to the huma NOVX nucleic acids disclosed herein using the human cDNAs, or a portion thereof, as a hybridization probe according to standard hybridization techniques under stringent hybridization conditions. For example, a soluble huma NOVX cDNA can be isolated based on its homology to human membrane-bound NOVX. Likewise, a membrane-bound huma NOVX cDNA can be isolated based on its homology to soluble huma NOVX. Accordingly, in another embodiment, an isolated nucleic acid molecule of the invention is at least 6 nucleotides in length and hybridizes under stringent conditions to the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23. In another embodiment, the nucleic acid is at least 10, 25, 50, 100, 250, 500 or 750 nucleotides in length. In another embodiment, an isolated nucleic acid molecule of the invention hybridizes to the coding region. As used herein, the term "hybridizes under stringent conditions" is intended to describe conditions for hybridization and washing under which nucleotide sequences at least 60% homologous to each other typically remain hybridized to each other.
Homologs (i.e., nucleic acids encoding NOVX proteins derived from species other than human) or other related sequences (e.g., paralogs) can be obtained by low, moderate or high stringency hybridization with all or a portion of the particular human sequence as a probe using methods well known in the art for nucleic acid hybridization and cloning. As used herein, the phrase "stringent hybridization conditions" refers to conditions under which a probe, primer or oligonucleotide will hybridize to its target sequence, but to no other sequences. Stringent conditions are sequence-dependent and will be different in different circumstances. Longer sequences hybridize specifically at higher temperatures than shorter sequences. Generally, stringent conditions are selected to be about 5°C lower than the thermal melting point (T- for the specific sequence at a defined ionic strength and pH. The Tm is the temperature (under defined ionic strength, pH and nucleic acid concentration) at which 50% of the probes complementary to the target sequence hybridize to the target sequence at equilibrium. Since the target sequences are generally present at excess, at Tm, 50% of the probes are occupied at equilibrium. Typically, stringent conditions will be those in which the salt concentration is less than about 1.0 M sodium ion, typically about 0.01 to 1.0 M sodium ion (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30°C for short probes, primers or oligonucleotides (e.g., 10 nt to 50 nt) and at least about 60°C for longer probes, primers and oligonucleotides. Stringent conditions may also be achieved with the addition of destabilizing agents, such as formamide.
Stringent conditions are known to those skilled in the art and can be found in CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, NN. (1989), 6.3.1-6.3.6. Preferably, the conditions are such that sequences at least about 65%, 70%, 75%, 85%, 90%), 95%, 98%, or 99% homologous to each other typically remain hybridized to each other. A non-limiting example of stringent hybridization conditions is hybridization in a high salt buffer comprising 6X SSC, 50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.02% BSA, and 500 mg/ml denatured salmon sperm DΝA at 65°C. This hybridization is followed by one or more washes in 0.2X SSC, 0.01% BSA at 50°C. An isolated nucleic acid molecule of the invention that hybridizes under stringent conditions to the sequence of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 corresponds to a naturally occurring nucleic acid molecule. As used herein, a "naturally-occurring" nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that occurs in nature (e.g., encodes a natural protein).
In a second embodiment, a nucleic acid sequence that is hybridizable to the nucleic acid molecule comprising the nucleotide sequence of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or fragments, analogs or derivatives thereof, under conditions of moderate stringency is provided. A non-limiting example of moderate stringency hybridization conditions are hybridization in 6X SSC, 5X Denhardt's solution, 0.5% SDS and 100 mg/ml denatured salmon sperm DNA at 55°C, followed by one or more washes in IX SSC, 0.1 % SDS at 37°C. Other conditions of moderate stringency that may be used are well known in the art. See, e.g., Ausubel et al. feds.), 1993, CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, NY, and Kriegler, 1990, GENE TRANSFER AND EXPRESSION, A LABORATORY MANUAL, Stockton Press, NY.
In a third embodiment, a nucleic acid that is hybridizable to the nucleic acid molecule comprising the nucleotide sequence of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or fragments, analogs or derivatives thereof, under conditions of low stringency, is provided. A non-limiting example of low stringency hybridization conditions are hybridization in 35% formamide, 5X SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 mg/ml denatured salmon sperm DNA, 10% (wt/vol) dextran sulfate at 40°C, followed by one or more washes in 2X SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS at 50°C. Other conditions of low stringency that may be used are well known in the art (e.g., as employed for cross-species hybridizations). See, e.g., Ausubel et al. (eds.), 1993, CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, NY, and Kriegler, 1990, GENE TRANSFER AND EXPRESSION, A LABORATORY MANUAL, Stockton Press, NY; Shilo and Weinberg, 1981, Proc Natl Acad Sci USA 78: 6789-6792.
Conservative mutations In addition to naturally-occurring allelic variants of the NOVX sequence that may exist in the population, the skilled artisan will further appreciate that changes can be introduced by mutation into the nucleotide sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, thereby leading to changes in the amino acid sequence of the encoded NOVX protein, without altering the functional ability of the NOVX protein. For example, nucleotide substitutions leading to amino acid substitutions at "non-essential" amino acid residues can be made in the sequence of SEQ ED NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23. A "non-essential" amino acid residue is a residue that can be altered from the wild-type sequence of NOVX without altering the biological activity, whereas an "essential" amino acid residue is required for biological activity. For example, amino acid residues that are conserved among the NOVX proteins of the present invention, are predicted to be particularly unamenable to alteration. Another aspect of the invention pertains to nucleic acid molecules encoding NOVX proteins that contain changes in amino acid residues that are not essential for activity. Such NOVX proteins differ in amino acid sequence from SEQ LD NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24, yet retain biological activity. In one embodiment, the isolated nucleic acid molecule comprises a nucleotide sequence encoding a protein, wherein the protein comprises an amino acid sequence at least about 75% homologous to the amino acid sequence of SEQ LD NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24 . Preferably, the protein encoded by the nucleic acid is at least about 80% homologous to SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24, more preferably at least about 90%, 95%, 98%, and most preferably at least about 99% homologous to SEQ LD NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24.
An isolated nucleic acid molecule encoding a NOVX protein homologous to the protein of can be created by introducing one or more nucleotide substitutions, additions or deletions into the nucleotide sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, such that one or more amino acid substitutions, additions or deletions are introduced into the encoded protein.
Mutations can be introduced into the nucleotide sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 by standard techniques, such as site-directed mutagenesis and PCR-mediated mutagenesis. Preferably, conservative amino acid substitutions are made at one or more predicted non-essential amino acid residues. A "conservative amino acid substitution" is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains (e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains (e.g., alanine, valine, leucine, isoleucine, praline, phenylalanine, methionine, tryptophan), beta-branched side chains (e.g., threonine, valine, isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine). Thus, a predicted nonessential amino acid residue in NOVX is replaced with another amino acid residue from the same side chain family. Alternatively, in another embodiment, mutations can be introduced randomly along all or part of a NOVX coding sequence, such as by saturation mutagenesis, and the resultant mutants can be screened for NOVX biological activity to identify mutants that retain activity. Following mutagenesis of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 the encoded protein can be expressed by any recombinant technology known in the art and the activity of the protein can be determined.
In one embodiment, a mutant NOVX protein can be assayed for (1) the ability to form proteimprotein interactions with other NOVX proteins, other cell-surface proteins, or biologically active portions thereof, (2) complex formation between a mutant NOVX protein and a NOVX receptor; (3) the ability of a mutant NOVX protein to bind to an intracellular target protein or biologically active portion thereof; (e.g., avidin proteins); (4) the ability to bind NOVX protein; or (5) the ability to specifically bind an anti-NOVX protein antibody.
Antisense NOVX Nucleic Acids
Another aspect of the invention pertains to isolated antisense nucleic acid molecules that are hybridizable to or complementary to the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or fragments, analogs or derivatives thereof. An "antisense" nucleic acid comprises a nucleotide sequence that is complementary to a "sense" nucleic acid encoding a protein, e.g., complementary to the coding strand of a double-stranded cDNA molecule or complementary to an mRNA sequence. In specific aspects, antisense nucleic acid molecules are provided that comprise a sequence complementary to at least about 10, 25, 50, 100, 250 or 500 nucleotides or an entire NOVX coding strand, or to only a portion thereof. Nucleic acid molecules encoding fragments, homologs, derivatives and analogs of a NOVX protein of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24 or antisense nucleic acids complementary to a NOVX nucleic acid sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 are additionally provided. In one embodiment, an antisense nucleic acid molecule is antisense to a "coding region" of the coding strand of a nucleotide sequence encoding NOVX. The term "coding region" refers to the region of the nucleotide sequence comprising codons which are translated into amino acid residues (e.g., the protein coding region of huma NOVX corresponds to SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24). In another embodiment, the antisense nucleic acid molecule is antisense to a "noncoding region" of the coding strand of a nucleotide sequence encoding NOVX. The term "noncoding region" refers to 5' and 3' sequences which flank the coding region that are not translated into amino acids (i.e., also referred to as 5' and 3' untranslated regions).
Given the coding strand sequences encoding NOVX disclosed herein (e.g., SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23), antisense nucleic acids of the invention can be designed according to the rules of Watson and Crick or Hoogsteen base pairing. The antisense nucleic acid molecule can be complementary to the entire coding region of NOVX mRNA, but more preferably is an oligonucleotide that is antisense to only a portion of the coding or noncoding region of NOVX mRNA. For example, the antisense oligonucleotide can be complementary to the region surrounding the translation start site of NOVX mRNA. An antisense oligonucleotide can be, for example, about 5, 10, 15, 20, 25, 30, 35, 40, 45 or 50 nucleotides in length. An antisense nucleic acid of the invention can be constructed using chemical synthesis or enzymatic ligation reactions using procedures known in the art. For example, an antisense nucleic acid (e.g., an antisense oligonucleotide) can be chemically synthesized using naturally occurring nucleotides or variously modified nucleotides designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between the antisense and sense nucleic acids, e.g., phosphorothioate derivatives and acridine substituted nucleotides can be used. Examples of modified nucleotides that can be used to generate the antisense nucleic acid include: 5-fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4-acetylcytosine, 5-(carboxyhydroxylmethyl) uracil, 5-carboxymethylaminomethyl- 2-thiouridine, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-D-galactosylqueosine, inosine, N6-isopentenyladenine, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 5-methylcytosine, N6-adenine, 7-methylguanine, 5-methylaminomethyluracil, 5-methoxyaminomethyl-2-thiouracil, beta-D-mannosylqueosine, 5'-methoxycarboxymethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid (v), wybutoxosine, pseudouracil, queosine, 2-thiocytosine, 5-methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid (v), 5-methyl-2-thiouracil,
3-(3-amino-3-N-2-carboxypropyl) uracil, (acp3)w, and 2,6-diaminopurine. Alternatively, the antisense nucleic acid can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation (i.e., RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest, described further in the following subsection).
The antisense nucleic acid molecules of the invention are typically administered to a subject or generated in situ such that they hybridize with or bind to cellular mRNA and/or genomic DNA encoding a NOVX protein to thereby inhibit expression of the protein, e.g., by inhibiting transcription and/or translation. The hybridization can be by conventional nucleotide complementarity to form a stable duplex, or, for example, in the case of an antisense nucleic acid molecule that binds to DNA duplexes, through specific interactions in the major groove of the double helix. An example of a route of administration of antisense nucleic acid molecules of the invention includes direct injection at a tissue site. Alternatively, antisense nucleic acid molecules can be modified to target selected cells and then administered systemically. For example, for systemic administration, antisense molecules can be modified such that they specifically bind to receptors or antigens expressed on a selected cell surface, e.g., by linking the antisense nucleic acid molecules to peptides or antibodies that bind to cell surface receptors or antigens. The antisense nucleic acid molecules can also be delivered to cells using the vectors described herein. To achieve sufficient intracellular concentrations of antisense molecules, vector constructs in which the antisense nucleic acid molecule is placed under the control of a strong pol II or pol III promoter are preferred.
In yet another embodiment, the antisense nucleic acid molecule of the invention is an α-anomeric nucleic acid molecule. An α-anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual β-units, the strands run parallel to each other (Gaultier et al. (1987) Nucleic Acids Res 15: 6625-6641). The antisense nucleic acid molecule can also comprise a 2'-o-methylribonucleotide (Inoue et al (1987) Nucleic Acids Res 15: 6131-6148) or a chimeric RNA -DNA analogue (Inoue et al (1987) FEBSLett 215: 327-330).
Such modifications include, by way of nonlimiting example, modified bases, and nucleic acids whose sugar phosphate backbones are modified or derivatized. These modifications are carried out at least in part to enhance the chemical stability of the modified nucleic acid, such that they may be used, for example, as antisense binding nucleic acids in therapeutic applications in a subject.
NOVX Ribozymes and PNA moieties
In still another embodiment, an antisense nucleic acid of the invention is a ribozyme.
Ribozymes are catalytic RNA molecules with ribonuclease activity that are capable of cleaving a single-stranded nucleic acid, such as a mRNA, to which they have a complementary region. Thus, ribozymes (e.g., hammerhead ribozymes (described in Haselhoff and Gerlach (1988) Nature 334:585-591)) can be used to catalytically cleave NOVX mRNA transcripts to thereby inhibit translation of NOVX mRNA. A ribozyme having specificity for a NOVX-encoding nucleic acid can be designed based upon the nucleotide sequence of a NOVX DNA disclosed herein (i.e., SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23). For example, a derivative of a Tetrahymena L-19 IVS RNA can be constructed in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be cleaved in a NOVX-encoding mRNA. See, e.g., Cech et al. U.S. Pat. No. 4,987,071; and Cech et al. U.S. Pat. No. 5,116,742. Alternatively, NOVX mRNA can be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA molecules. See, e.g., Bartel et al, (1993) -Science 261:1411-1418.
Alternatively, NOVX gene expression can be inhibited by targeting nucleotide sequences complementary to the regulatory region of the NOVX (e.g. , the NOVX promoter and/or enhancers) to form triple helical structures that prevent transcription of the NOVX gene in target cells. See generally, Helene. (1991) Anticancer DrugDes. 6: 569-84; Helene. et al. (1992) Ann. NY. Acad. Sci. 660:27-36; and Maher (1992) Bioassays 14: 807-15.
In various embodiments, the nucleic acids of NOVX can be modified at the base moiety, sugar moiety or phosphate backbone to improve, e.g. , the stability, hybridization, or solubility of the molecule. For example, the deoxyribose phosphate backbone of the nucleic acids can be modified to generate peptide nucleic acids (see Hyrup et al. (1996) BioorgMed Chem 4: 5-23). As used herein, the terms "peptide nucleic acids" or "PNAs" refer to nucleic acid mimics, e.g., DNA mimics, in which the deoxyribose phosphate backbone is replaced by a pseudopeptide backbone and only the four natural nucleobases are retained. The neutral backbone of PNAs has been shown to allow for specific hybridization to DNA and RNA under conditions of low ionic strength. The synthesis of PNA oligomers can be performed using standard solid phase peptide synthesis protocols as described in Hyrup et al. (1996) above; Perry-O'Keefe et β/. (1996) EN S 93: 14670-675. PΝAs of ΝOVX can be used in therapeutic and diagnostic applications. For example,
PΝAs can be used as antisense or antigene agents for sequence-specific modulation of gene expression by, e.g., inducing transcription or translation arrest or inhibiting replication. PΝAs of ΝOVX can also be used, e.g., in the analysis of single base pair mutations in a gene by, e.g., PΝA directed PCR clamping; as artificial restriction enzymes when used in combination with other enzymes, e.g., SI nucleases (Hyrup B. (1996) above); or as probes or primers for DΝA sequence and hybridization (Hyrup et al. (1996), above; Perry-O'Keefe (1996), above). In another embodiment, PΝAs of ΝOVX can be modified, e.g., to enhance their stability or cellular uptake, by attaching lipophilic or other helper groups to PΝA, by the formation of PΝA-DΝA chimeras, or by the use of liposomes or other techniques of drug delivery known in the art. For example, PΝA-DΝA chimeras of ΝOVX can be generated that may combine the advantageous properties of PΝA and DΝA. Such chimeras allow DΝA recognition enzymes, e.g., RΝase H and DΝA polymerases, to interact with the DΝA portion while the PΝA portion would provide high binding affinity and specificity. PΝA-DΝA chimeras can be linked using linkers of appropriate lengths selected in terms of base stacking, number of bonds between the nucleobases, and orientation (Hyrup (1996) above). The synthesis of PNA-DNA chimeras can be performed as described in Hyrup (1996) above and Finn et al. (1996) Nucl Acids Res 24: 3357-63. For example, a DNA chain can be synthesized on a solid support using standard phosphoramidite coupling chemistry, and modified nucleoside analogs, e.g., 5'-(4-methoxytrityl) amino-5'-deoxy-thymidine phosphoramidite, can be used between the PNA and the 5' end of DNA (Mag et al. (1989) Nucl Acid Res 17: 5973-88). PNA monomers are then coupled in a stepwise manner to produce a chimeric molecule with a 5' PNA segment and a 3' DNA segment (Finn et al. (1996) above). Alternatively, chimeric molecules can be synthesized with a 5' DNA segment and a 3' PNA segment. See, Petersen et al. (1975) Bioorg Med Chem Lett 5: 1119-11124.
In other embodiments, the oligonucleotide may include other appended groups such as peptides (e.g., for targeting host cell receptors in vivo), or agents facilitating transport across the cell membrane (see, e.g., Letsinger et al, 1989, Proc. Natl. Acad. Sci. U.S.A. 86:6553-6556; Lemaifre et al, 1987, Proc. Natl. Acad. Sci. 84:648-652; PCT Publication No. W088/09810) or the blood-brain barrier (see, e.g., PCT Publication No. W089/10134). In addition, oligonucleotides can be modified with hybridization triggered cleavage agents (See, e.g., Krol et al, 1988, BioTechniques 6:958-976) or intercalating agents. (See, e.g., Zon, 1988, Pharm. Res. 5: 539-549). To this end, the oligonucleotide may be conjugated to another molecule, e.g., a peptide, a hybridization triggered cross-linking agent, a transport agent, a hybridization-triggered cleavage agent, etc.
NOVX Polypeptides
A NOVX polypeptide of the invention includes the NOVX-like protein whose sequence is provided SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24. The invention also includes a mutant or variant protein any of whose residues may be changed from the corresponding residue shown SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24 while still encoding a protein that maintains its NOVX-like activities and physiological functions, or a functional fragment thereof. In some embodiments, up to 20% or more of the residues may be so changed in the mutant or variant protein, hi some embodiments, the NOVX polypeptide according to the invention is a mature polypeptide.
In general, a NOVX -like variant that preserves NOVX-like function includes any variant in which residues at a particular position in the sequence have been substituted by other amino acids, and further include the possibility of inserting an additional residue or residues between two residues of the parent protein as well as the possibility of deleting one or more residues from the parent sequence. Any amino acid substitution, insertion, or deletion is encompassed by the invention. In favorable circumstances, the substitution is a conservative substitution as defined above.
One aspect of the invention pertains to isolated NOVX proteins, and biologically active portions thereof, or derivatives, fragments, analogs or homologs thereof. Also provided are polypeptide fragments suitable for use as immunogens to raise anti-NOVX antibodies. In one embodiment, native NOVX proteins can be isolated from cells or tissue sources by an appropriate purification scheme using standard protein purification techniques. In another embodiment, NOVX proteins are produced by recombinant DNA techniques. Alternative to recombinant expression, a NOVX protein or polypeptide can be synthesized chemically using standard peptide synthesis techniques.
An "isolated" or "purified" protein or biologically active portion thereof is substantially free of cellular material or other contaminating proteins from the cell or tissue source from which the NOVX protein is derived, or substantially free from chemical precursors or other chemicals when chemically synthesized. The language "substantially free of cellular material" includes preparations of NOVX protein in which the protein is separated from cellular components of the cells from which it is isolated or recombinantly produced. In one embodiment, the language "substantially free of cellular material" includes preparations of NOVX protein having less than about 30% (by dry weight) of non-NOVX protein (also referred to herein as a "contaminating protein"), more preferably less than about 20% of non-NOVX protein, still more preferably less than about 10% of non-NOVX protein, and most preferably less than about 5% non-NOVX protein. When the NOVX protein or biologically active portion thereof is recombinantly produced, it is also preferably substantially free of culture medium, i.e., culture medium represents less than about 20%, more preferably less than about 10%, and most preferably less than about 5% of the volume of the protein preparation. The language "substantially free of chemical precursors or other chemicals" includes preparations of NOVX protein in which the protein is separated from chemical precursors or other chemicals that are involved in the synthesis of the protein. In one embodiment, the language "substantially free of chemical precursors or other chemicals" includes preparations of NOVX protein having less than about 30% (by dry weight) of chemical precursors or non-NOVX chemicals, more preferably less than about 20%o chemical precursors or non-NOVX chemicals, still more preferably less than about 10% chemical precursors or non-NOVX chemicals, and most preferably less than about 5%> chemical precursors or non-NOVX chemicals.
Biologically active portions of a NOVX protein include peptides comprising amino acid sequences sufficiently homologous to or derived from the amino acid sequence of the NOVX protein, e.g., the amino acid sequence shown SEQ LD NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24 that include fewer amino acids than the full length NOVX proteins, and exhibit at least one activity of a NOVX protein. Typically, biologically active portions comprise a domain or motif with at least one activity of the NOVX protein. A biologically active portion of a NOVX protein can be a polypeptide which is, for example, 10, 25, 50, 100 or more amino acids in length.
A biologically active portion of a NOVX protein of the present invention may contain at least one of the above-identified domains conserved between the NOVX proteins, e.g. TSR modules. Moreover, other biologically active portions, in which other regions of the protein are deleted, can be prepared by recombinant techniques and evaluated for one or more of the functional activities of a native NOVX protein.
In an embodiment, the NOVX protein has an amino acid sequence shown SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24. In other embodiments, the NOVX protein is substantially homologous to SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24 and retains the functional activity of the protein of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24 yet differs in amino acid sequence due to natural allelic variation or mutagenesis, as described in detail below. Accordingly, in another embodiment, the NOVX protein is a protem that comprises an amino acid sequence at least about 45% homologous to the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24 and retains the functional activity of the NOVX proteins of SEQ LD NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24.
Determining homology between two or more sequences
To determine the percent homology of two amino acid sequences or of two nucleic acids, the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in either of the sequences being compared for optimal alignment between the sequences). The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in the first sequence is occupied by the same amino acid residue or nucleotide as the corresponding position in the second sequence, then the molecules are homologous at that position (i.e., as used herein amino acid or nucleic acid "homology" is equivalent to amino acid or nucleic acid "identity").
The nucleic acid sequence homology may be determined as the degree of identity between two sequences. The homology may be determined using computer programs known in the art, such as GAP software provided in the GCG program package. See, Needleman and Wunsch 1970 J Mol Biol 48: 443-453. Using GCG GAP software with the following settings for nucleic acid sequence comparison: GAP creation penalty of 5.0 and GAP extension penalty of 0.3, the coding region of the analogous nucleic acid sequences referred to above exhibits a degree of identity preferably of at least 70%, 75%, 80%, 85%, 90%, 95%, 98%, or 99%, with the CDS (encoding) part of the DNA sequence shown in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23.
The term "sequence identity" refers to the degree to which two polynucleotide or polypeptide sequences are identical on a residue-by-residue basis over a particular region of comparison. The term "percentage of sequence identity" is calculated by comparing two optimally aligned sequences over that region of comparison, determining the number of positions at which the identical nucleic acid base (e.g., A, T, C, G, U, or I, in the case of nucleic acids) occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the region of comparison (i.e., the window size), and multiplying the result by 100 to yield the percentage of sequence identity. The term "substantial identity" as used herein denotes a characteristic of a polynucleotide sequence, wherein the polynucleotide comprises a sequence that has at least 80 percent sequence identity, preferably at least 85 percent identity and often 90 to 95 percent sequence identity, more usually at least 99 percent sequence identity as compared to a reference sequence over a comparison region. The term "percentage of positive residues" is calculated by comparing two optimally aligned sequences over that region of comparison, determining the number of positions at which the identical and conservative amino acid substitutions, as defined above, occur in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the region of comparison (i.e., the window size), and multiplying the result by 100 to yield the percentage of positive residues. Chimeric and fusion proteins
The invention also provides NOVX chimeric or fusion proteins. As used herein, a
NOVX "chimeric protein" or "fusion protein" comprises a NOVX polypeptide operatively linked to a non-NOVX polypeptide. An "NOVX polypeptide" refers to a polypeptide having an amino acid sequence corresponding to NOVX, whereas a "non-NOVX polypeptide" refers to a polypeptide having an amino acid sequence corresponding to a protein that is not substantially homologous to the NOVX protein, e.g., a protein that is different from the NOVX protein and that is derived from the same or a different organism. Within a NOVX fusion protein the NOVX polypeptide can correspond to all or a portion of a NOVX protein. In one embodiment, a NOVX fusion protein comprises at least one biologically active portion of a NOVX protein. In another embodiment, a NOVX fusion protein comprises at least two biologically active portions of a NOVX protein. Within the fusion protein, the term "operatively linked" is intended to indicate that the NOVX polypeptide and the non-NOVX polypeptide are fused in-frame to each other. The non-NOVX polypeptide can be fused to the N-terminus or C-terminus of the NOVX polypeptide.
For example, in one embodiment a NOVX fusion protein comprises a NOVX polypeptide operably linked to the extracellular domain of a second protein. Such fusion proteins can be further utilized in screening assays for compounds that modulate NOVX activity (such assays are described in detail below). In another embodiment, the fusion protein is a GST-NO VX fusion protein in which the
NOVX sequences are fused to the C-terminus of the GST (i.e., glutathione S-transferase) sequences. Such fusion proteins can facilitate the purification of recombinant NOVX.
In another embodiment, the fusion protein is a NOVX-immunoglobulin fusion protein in which the NOVX sequences comprising one or more domains are fused to sequences derived from a member of the immunoglobulin protein family. The NOVX-immunoglobulin fusion proteins of the invention can be incorporated into pharmaceutical compositions and administered to a subject to inhibit an interaction between a NOVX ligand and a NOVX protein on the surface of a cell, to thereby suppress NOVX-mediated signal transduction in vivo. In one nonlimiting example, a contemplated NOVX ligand of the invention is the NOVX receptor. The NOVX-immunoglobulin fusion proteins can be used to affect the bioavailability of a NOVX cognate ligand. Inhibition of the NOVX ligand/NOVX interaction may be useful therapeutically for both the treatment of proliferative and differentiative disorders, e,g., cancer as well as modulating (e.g., promoting or inhibiting) cell survival, as well as acute and chronic inflammatory disorders and hyperplastic wound healing, e.g. hypertrophic scars and keloids. Moreover, the NOVX-immunoglobulin fusion proteins of the invention can be used as immunogens to produce anti-NOVX antibodies in a subject, to purify NOVX ligands, and in screening assays to identify molecules that inhibit the interaction of NOVX with a NOVX ligand.
A NOVX chimeric or fusion protein of the invention can be produced by standard recombinant DNA techniques. For example, DNA fragments coding for the different polypeptide sequences are ligated together in-frame in accordance with conventional techniques, e.g., by employing blunt-ended or stagger-ended termini for ligation, restriction enzyme digestion to provide for appropriate termini, filling-in of cohesive ends as appropriate, alkaline phosphatase treatment to avoid undesirable joining, and enzymatic ligation. In another embodiment, the fusion gene can be synthesized by conventional techniques including automated DNA synthesizers. Alternatively, PCR amplification of gene fragments can be carried out using anchor primers that give rise to complementary overhangs between two consecutive gene fragments that can subsequently be annealed and reamplified to generate a chimeric gene sequence (see, for example, Ausubel et al. (eds.) CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, 1992). Moreover, many expression vectors are commercially available that already encode a fusion moiety (e.g., a GST polypeptide). A NOVX-encoding nucleic acid can be cloned into such an expression vector such that the fusion moiety is linked in-frame to the NOVX protein.
NOVX agonists and antagonists
The present invention also pertains to variants of the NOVX proteins that function as either NOVX agonists (mimetics) or as NOVX antagonists. Variants of the NOVX protein can be generated by mutagenesis, e.g., discrete point mutation or truncation of the NOVX protein. An agonist of the NOVX protein can retain substantially the same, or a subset of, the biological activities of the naturally occurring form of the NOVX protein. An antagonist of the NOVX protein can inhibit one or more of the activities of the naturally occurring form of the NOVX protein by, for example, competitively binding to a downstream or upstream member of a cellular signaling cascade which includes the NOVX protein. Thus, specific biological effects can be elicited by treatment with a variant of limited function. In one embodiment, treatment of a subject with a variant having a subset of the biological activities of the naturally occurring form of the protein has fewer side effects in a subject relative to treatment with the naturally occurring form of the NOVX proteins.
Variants of the NOVX protein that function as either NOVX agonists (mimetics) or as NOVX antagonists can be identified by screening combinatorial libraries of mutants, e.g., truncation mutants, of the NOVX protein for NOVX protein agonist or antagonist activity. In one embodiment, a variegated library of NOVX variants is generated by combinatorial mutagenesis at the nucleic acid level and is encoded by a variegated gene library. A variegated library of NOVX variants can be produced by, for example, enzymatically ligating a mixture of synthetic oligonucleotides into gene sequences such that a degenerate set of potential NOVX sequences is expressible as individual polypeptides, or alternatively, as a set of larger fusion proteins (e.g., for phage display) containing the set of NOVX sequences therein. There are a variety of methods which can be used to produce libraries of potential NOVX variants from a degenerate oligonucleotide sequence. Chemical synthesis of a degenerate gene sequence can be performed in an automatic DNA synthesizer, and the synthetic gene then ligated into an appropriate expression vector. Use of a degenerate set of genes allows for the provision, in one mixture, of all of the sequences encoding the desired set of potential NOVX sequences. Methods for synthesizing degenerate oligonucleotides are known in the art (see, e.g., Narang (1983) Tetrahedron 39:3; Itakura et al. (1984) Annu Rev Biochem 53:323; Itakura et al. (1984) Science 198:1056; Lke et al. (1983) Nucl Acid Res 11:477.
Polypeptide libraries
In addition, libraries of fragments of the NOVX protein coding sequence can be used to generate a variegated population of NOVX fragments for screening and subsequent selection of variants of a NOVX protein. In one embodiment, a library of coding sequence fragments can be generated by treating a double stranded PCR fragment of a NOVX coding sequence with a nuclease under conditions wherein nicking occurs only about once per molecule, denaturing the double stranded DNA, renaturing the DNA to form double stranded DNA that can include sense/antisense pairs from different nicked products, removing single stranded portions from reformed duplexes by treatment with SI nuclease, and ligating the resulting fragment library into an expression vector. By this method, an expression library can be derived which encodes N-terminal and internal fragments of various sizes of the NOVX protein. Several techniques are known in the art for screening gene products of combinatorial libraries made by point mutations or truncation, and for screening cDNA libraries for gene products having a selected property. Such techniques are adaptable for rapid screening of the gene libraries generated by the combinatorial mutagenesis of NOVX proteins. The most widely used techniques, which are amenable to high throughput analysis, for screening large gene libraries typically include cloning the gene library into replicable expression vectors, transforming appropriate cells with the resulting library of vectors, and expressing the combinatorial genes under conditions in which detection of a desired activity facilitates isolation of the vector encoding the gene whose product was detected. Recrusive ensemble mutagenesis (REM), a new technique that enhances the frequency of functional mutants in the libraries, can be used in combination with the screening assays to identify NOVX variants (Arkin and Yourvan (1992) PNAS 89:7811-7815; Delgrave et al. (1993) Protein Engineering 6:327-331).
NOVX Antibodies
Also included in the invention are antibodies to NOVX proteins, or fragments of
NOVX proteins. The term "antibody" as used herein refers to immunoglobulin molecules and immunologically active portions of immxmoglobulin (Ig) molecules, i.e., molecules that contain an antigen binding site that specifically binds (immunoreacts with) an antigen. Such antibodies include, but are not limited to, polyclonal, monoclonal, chimeric, single chain, Fab, Fab. and F(ab.)2 fragments, and an Fab expression library. In general, an antibody molecule obtained from humans relates to any of the classes IgG, IgM, IgA, IgE and IgD, which differ from one another by the nature of the heavy chain present in the molecule. Certain classes . have subclasses as well, such as IgGl5 IgG2, and others. Furthermore, in humans, the light chain may be a kappa chain or a lambda chain. Reference herein to antibodies includes a reference to all such classes, subclasses and types of human antibody species.
An isolated NOVX-related protein of the invention may be intended to serve as an antigen, or a portion or fragment thereof, and additionally can be used as an immunogen to generate antibodies that immunospecifically bind the antigen, using standard techniques for polyclonal and monoclonal antibody preparation. The full-length protein can be used or, alternatively, the invention provides antigenic peptide fragments of the antigen for use as immunogens. An antigenic peptide fragment comprises at least 6 amino acid residues of the amino acid sequence of the full length protein, such as an amino acid sequence shown in from SEQ LD NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 or 24, and encompasses an epitope thereof such that an antibody raised against the peptide forms a specific immune complex with the full length protein or with any fragment that contains the epitope. Preferably, the antigenic peptide comprises at least 10 amino acid residues, or at least 15 amino acid residues, or at least 20 amino acid residues, or at least 30 amino acid residues. Preferred epitopes encompassed by the antigenic peptide are regions of the protein that are located on its surface; commonly these are hydrophilic regions.
In certain embodiments of the invention, at least one epitope encompassed by the antigenic peptide is a region of NOVX-related protein that is located on the surface of the protein, e.g., a hydrophilic region. A hydrophobicity analysis of the huma NOVX-related protein sequence will indicate which regions of a NOVX-related protein are particularly hydrophilic and, therefore, are likely to encode surface residues useful for targeting antibody production. As a means for targeting antibody production, hydropathy plots showing regions of hydrophilicity and hydrophobicity may be generated by any method well known in the art, including, for example, the Kyte Doolittle or the Hopp Woods methods, either with or without Fourier transformation. See, e.g., Hopp and Woods, 1981, Proc. Nat. Acad. Sci. USA 78: 3824-3828; Kyte and Doolittle 1982, J. Mol. Biol. 157: 105-142, each of which is incorporated herein by reference in its entirety. Antibodies that are specific for one or more domains within an antigenic protein, or derivatives, fragments, analogs or homologs thereof, are also provided herein.
A protein of the invention, or a derivative, fragment, analog, homolog or ortholog thereof, may be utilized as an immunogen in the generation of antibodies that immunospecifically bind these protein components.
Various procedures known within the art may be used for the production of polyclonal or monoclonal antibodies directed against a protein of the invention, or against derivatives, fragments, analogs homologs or orthologs thereof (see, for example, Antibodies: A Laboratory Manual, Harlow E, and Lane D, 1988, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, incorporated herein by reference). Some of these antibodies are discussed below.
Polyclonal Antibodies
For the production of polyclonal antibodies, various suitable host animals (e.g., rabbit, goat, mouse or other mammal) may be immunized by one or more injections with the native protein, a synthetic variant thereof, or a derivative of the foregoing. An appropriate immunogenic preparation can contain, for example, the naturally occurring immunogenic protein, a chemically synthesized polypeptide representing the immunogenic protein, or a recombinantly expressed immunogenic protein. Furthermore, the protein may be conjugated to a second protein known to be immunogenic in the mammal being immunized. Examples of such immunogenic proteins include but are not limited to keyhole limpet hemocyanin, serum albumin, bovine thyroglobulin, and soybean trypsin inhibitor. The preparation can further include an adjuvant. Various adjuvants used to increase the immunological response include, but are not limited to, Freund's (complete and incomplete), mineral gels (e.g., aluminum hydroxide), surface active substances (e.g., lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, dinitrophenol, etc.), adjuvants usable in humans such as Bacille Calmette-Guerin and Corynebacterium parvum, or similar immunostimulatory agents. Additional examples of adjuvants which can be employed include MPL-TDM adjuvant (monophosphoryl Lipid A, synthetic trehalose dicorynomycolate).
The polyclonal antibody molecules directed against the immunogenic protein can be isolated from the mammal (e.g., from the blood) and further purified by well known techniques, such as affinity chromatography using protein A or protein G, which provide primarily the IgG fraction of immune serum. Subsequently, or alternatively, the specific antigen which is the target of the immxmoglobulin sought, or an epitope thereof, may be immobilized on a column to purify the immune specific antibody by immunoaffinity chromatography. Purification of immunoglobulins is discussed, for example, by D. Wilkinson (The Scientist, published by The Scientist, Inc., Philadelphia PA, Vol. 14, No. 8 (April 17, 2000), pp. 25-28).
Monoclonal Antibodies The term "monoclonal antibody" (MAb) or "monoclonal antibody composition", as used herein, refers to a population of antibody molecules that contain only one molecular species of antibody molecule consisting of a unique light chain gene product and a unique heavy chain gene product. In particular, the complementarity determining regions (CDRs) of the monoclonal antibody are identical in all the molecules of the population. MAbs thus contain an antigen binding site capable of immunoreacting with a particular epitope of the antigen characterized by a unique binding affinity for it.
Monoclonal antibodies can be prepared using hybridoma methods, such as those described by Kohler and Milstein, Nature, *256:495 (1975). In a hybridoma method, a mouse, hamster, or other appropriate host animal, is typically immunized with an immunizing agent to elicit lymphocytes that produce or are capable of producing antibodies that will specifically bind to the immunizing agent. Alternatively, the lymphocytes can be immunized in vitro.
The immunizing agent will typically include the protein antigen, a fragment thereof or a fusion protein thereof. Generally, either peripheral blood lymphocytes are used if cells of human origin are desired, or spleen cells or lymph node cells are used if non-human mammalian sources are desired. The lymphocytes are then fused with an immortalized cell line using a suitable fusing agent, such as polyethylene glycol, to form a hybridoma cell (Goding, Monoclonal Antibodies: Principles and Practice, Academic Press, (1986) pp. 59- 103). Immortalized cell lines are usually transformed mammalian cells, particularly myeloma cells of rodent, bovine and human origin. Usually, rat or mouse myeloma cell lines are employed. The hybridoma cells can be cultured in a suitable culture medium that preferably contains one or more substances that inhibit the growth or survival of the unfused, immortalized cells. For example, if the parental cells lack the enzyme hypoxanthine guanine phosphoribosyl transferase (HGPRT or HPRT), the culture medium for the hybridomas typically will include hypoxanthine, aminopterin, and thymidine ("HAT medium"), which substances prevent the growth of HGPRT-deficient cells.
Preferred immortalized cell lines are those that fuse efficiently, support stable high level expression of antibody by the selected antibody-producing cells, and are sensitive to a medium such as HAT medium. More preferred immortalized cell lines are murine myeloma lines, which can be obtained, for instance, from the Salk Institute Cell Distribution Center, San Diego, California and the American Type Culture Collection, Manassas, Virginia. Human myeloma and mouse-human heteromyeloma cell lines also have been described for the production of human monoclonal antibodies (Kozbor, J. Immunol., 133:3001 (1984); Brodeur et al., Monoclonal Antibody Production Techniques and Applications, Marcel Dekker, Inc., New York, (1987) pp. 51-63).
The culture medium in which the hybridoma cells are cultured can then be assayed for the presence of monoclonal antibodies directed against the antigen. Preferably, the binding specificity of monoclonal antibodies produced by the hybridoma cells is determined by immunoprecipitation or by an in vitro binding assay, such as radioimmunoassay (RIA) or enzyme-linked immunoabsorbent assay (ELISA). Such techniques and assays are known in the art. The binding affinity of the monoclonal antibody can, for example, be determined by the Scatchard analysis of Munson and Pollard, Anal. Biochem., 107:220 (1980). Preferably, antibodies having a high degree of specificity and a high binding affinity for the target antigen are isolated.
After the desired hybridoma cells are identified, the clones can be subcloned by limiting dilution procedures and grown by standard methods. Suitable culture media for this purpose include, for example, Dulbecco's Modified Eagle's Medium and RPMI-1640 medium. Alternatively, the hybridoma cells can be grown iv vivo as ascites in a mammal.
The monoclonal antibodies secreted by the subclones can be isolated or purified from the culture medium or ascites fluid by conventional immunoglobulin purification procedures such as, for example, protein A-Sepharose, hydroxylapatite chromatography, gel electrophoresis, dialysis, or affinity chromatography.
The monoclonal antibodies can also be made by recombinant DNA methods, such as those described in U.S. Patent No. 4,816,567. DNA encoding the monoclonal antibodies of the invention can be readily isolated and sequenced using conventional procedures (e.g., by using oligonucleotide probes that are capable of binding specifically to genes encoding the heavy and light chains of murine antibodies). The hybridoma cells of the invention serve as a preferred source of such DNA. Once isolated, the DNA can be placed into expression vectors, which are then transfected into host cells such as simian COS cells, Chinese hamster ovary (CHO) cells, or myeloma cells that do not otherwise produce immunoglobulin protein, to obtain the synthesis of monoclonal antibodies in the recombinant host cells. The DNA also can be modified, for example, by substituting the coding sequence for human heavy and light chain constant domains in place of the homologous murine sequences (U.S. Patent No. 4,816,567; Morrison, Nature 368, 812-13 (1994)) or by covalently joining to the immunoglobulin coding sequence all or part of the coding sequence for a non-immuno globulin polypeptide. Such a non-immunoglobulin polypeptide can be substituted for the constant domains of an antibody of the invention, or can be substituted for the variable domains of one antigen-combining site of an antibody of the invention to create a chimeric bivalent antibody.
Humanized Antibodies
The antibodies directed against the protein antigens of the invention can further comprise humanized antibodies or human antibodies. These antibodies are suitable for administration to humans without engendering an immune response by the human against the administered immunoglobulin. Humanized forms of antibodies are chimeric immunoglobulins, immunoglobulin chains or fragments thereof (such as Fv, Fab, Fab', F(ab')2 or other antigen- binding subsequences of antibodies) that are principally comprised of the sequence of a human immunoglobulin, and contain minimal sequence derived from a non-human immunoglobulin. Humanization can be performed following the method of Winter and co-workers (Jones et al., Nature. 321:522-525 (1986); Riechmann et al., Nature, 332:323-327 (1988); Verhoeyen et al., Science. 239:1534-1536 (1988)), by substituting rodent CDRs or CDR sequences for the corresponding sequences of a human antibody. (See also U.S. Patent No. 5,225,539.) In some instances, Fv framework residues of the human immunoglobulin are replaced by corresponding non-human residues. Humanized antibodies can also comprise residues which are found neither in the recipient antibody nor in the imported CDR or framework sequences. In general, the humanized antibody will comprise substantially all of at least one, and typically two, variable domains, in which all or substantially all of the CDR regions correspond to those of a non-human immunoglobulin and all or substantially all of the framework regions are those of a human immunoglobulin consensus sequence. The humanized antibody optimally also will comprise at least a portion of an immunoglobulin constant region (Fc), typically that of a human immunoglobulin (Jones et al., 1986; Riechmann et al., 1988; and Presta, Curr. Op. Struct. Biol.. 2:593-596 (1992)).
Human Antibodies
Fully human antibodies relate to antibody molecules in which essentially the entire sequences of both the light chain and the heavy chain, including the CDRs, arise from human genes. Such antibodies are termed "human antibodies", or "fully human antibodies" herein. Human monoclonal antibodies can be prepared by the trioma technique; the human B-cell hybridoma technique (see Kozbor, et al., 1983 Immunol Today 4: 72) and the EBV hybridoma technique to produce human monoclonal antibodies (see Cole, et al., 1985 In: MONOCLONAL ANTIBODIES AND CANCER THERAPY, Alan R. Liss, Inc., pp. 77-96). Human monoclonal antibodies may be utilized in the practice of the present invention and may be produced by using human hybridomas (see Cote, et al., 1983. Proc Natl Acad Sci USA 80: 2026-2030) or by transforming human B-cells with Epstein Barr Virus in vitro (see Cole, et al., 1985 In: MONOCLONAL ANTIBODIES AND CANCER THERAPY, Alan R. Liss, Inc., pp. 77-96). In addition, human antibodies can also be produced using additional techniques, including phage display libraries (Hoogenboom and Winter, J. Mol. Biol.. 227:381 (1991); Marks et al, J. Mol. Biol.. 222:581 (1991)). Similarly, human antibodies can be made by introducing human immunoglobulin loci into transgenic animals, e.g., mice in which the endogenous immunoglobulin genes have been partially or completely inactivated. Upon challenge, human antibody production is observed, which closely resembles that seen in humans in all respects, including gene rearrangement, assembly, and antibody repertoire. This approach is described, for example, in U.S. Patent Nos. 5,545,807; 5,545,806; 5,569,825; 5,625,126; 5,633,425; 5,661,016, and in Marks et al. (Bio/Technology 10, 779-783 (1992)); Lonberg et al. (Nature 368 856-859 (1994)); Morrison ( Nature 368. 812-13 (1994)); Fishwild et al,( Nature Biotechnology 14, 845-51 (1996)); Neuberger (Nature Biotechnology 14, 826 (1996)); and Lonberg and Huszar (Intern. Rev. Immunol. 13 65-93 (1995)).
Human antibodies may additionally be produced using transgenic nonhuman animals which are modified so as to produce fully human antibodies rather than the animal's endogenous antibodies in response to challenge by an antigen. (See PCT publication WO94/02602). The endogenous genes encoding the heavy and light immunoglobulin chains in the nonhuman host have been incapacitated, and active loci encoding human heavy and light chain immunoglobulins are inserted into the host's genome. The human genes are incorporated, for example, using yeast artificial chromosomes containing the requisite human DNA segments. An animal which provides all the desired modifications is then obtained as progeny by crossbreeding intermediate transgenic animals containing fewer than the full complement of the modifications. The preferred embodiment of such a nonhuman animal is a mouse, and is termed the Xenomouse™ as disclosed in PCT publications WO 96/33735 and WO 96/34096. This animal produces B cells which secrete fully human immunoglobulins. The antibodies can be obtained directly from the animal after immunization with an immunogen of interest, as, for example, a preparation of a polyclonal antibody, or alternatively from immortalized B cells derived from the animal, such as hybridomas producing monoclonal antibodies. Additionally, the genes encoding the immunoglobulins with human variable regions can be recovered and expressed to obtain the antibodies directly, or can be further modified to obtain analogs of antibodies such as, for example, single chain Fv molecules.
An example of a method of producing a nonhuman host, exemplified as a mouse, lacking expression of an endogenous immunoglobulin heavy chain is disclosed in U.S. Patent No. 5,939,598. It can be obtained by a method including deleting the J segment genes from at least one endogenous heavy chain locus in an embryonic stem cell to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged immunoglobulin heavy chain locus, the deletion being effected by a targeting vector containing a gene encoding a selectable marker; and producing from the embryonic stem cell a transgenic mouse whose somatic and germ cells contain the gene encoding the selectable marker.
A method for producing an antibody of interest, such as a human antibody, is disclosed in U.S. Patent No. 5,916,771. It includes introducing an expression vector that contains a nucleotide sequence encoding a heavy chain into one mammalian host cell in culture, introducing an expression vector containing a nucleotide sequence encoding a light chain into another mammalian host cell, and fusing the two cells to form a hybrid cell. The hybrid cell expresses an antibody containing the heavy chain and the light chain.
In a further improvement on this procedure, a method for identifying a clinically relevant epitope on an immunogen, and a correlative method for selecting an antibody that binds immunospecifically to the relevant epitope with high affinity, are disclosed in PCT publication WO 99/53049.
Fab Fragments and Single Chain Antibodies According to the invention, techniques can be adapted for the production of single-chain antibodies specific to an antigenic protein of the invention (see e.g., U.S. Patent No. 4,946,778). In addition, methods can be adapted for the construction of Fab expression libraries (see e.g., Huse, et al., 1989 Science 246: 1275-1281) to allow rapid and effective identification of monoclonal Fab fragments with the desired specificity for a protein or derivatives, fragments, analogs or homologs thereof. Antibody fragments that contain the idiotypes to a protein antigen may be produced by techniques known in the art including, but not limited to: (i) an F(ab,)2 fragment produced by pepsin digestion of an antibody molecule; (ii) an Fab fragment generated by reducing the disulfide bridges of an F(ab,)2 fragment; (iii) an Fab fragment generated by the treatment of the antibody molecule with papain and a reducing agent and (iv) Fv fragments.
Bispecific Antibodies
Bispecific antibodies are monoclonal, preferably human or humanized, antibodies that have binding specificities for at least two different antigens. In the present case, one of the binding specificities is for an antigenic protein of the invention. The second binding target is any other antigen, and advantageously is a cell-surface protein or receptor or receptor subunit.
Methods for making bispecific antibodies are known in the art. Traditionally, the recombinant production of bispecific antibodies is based on the co-expression of two immunoglobulin heavy-chain/light-chain pairs, where the two heavy chains have different specificities (Milstein and Cuello, Nature. 305:537-539 (1983)). Because of the random assortment of immunoglobulin heavy and light chains, these hybridomas (quadromas) produce a potential mixture often different antibody molecules, of which only one has the correct bispecific structure. The purification of the correct molecule is usually accomplished by affinity chromatography steps. Similar procedures are disclosed in WO 93/08829, published 13 May 1993, and in Traunecker et al, 1991 EMBO , 10:3655-3659.
Antibody variable domains with the desired binding specificities (antibody-antigen combining sites) can be fused to immunoglobulin constant domain sequences. The fusion preferably is with an immunoglobulin heavy-chain constant domain, comprising at least part of the hinge, CH2, and CH3 regions. It is preferred to have the first heavy-chain constant region (CHI) containing the site necessary for light-chain binding present in at least one of the fusions. DNAs encoding the immunoglobulin heavy-chain fusions and, if desired, the immunoglobulin light chain, are inserted into separate expression vectors, and are co- transfected into a suitable host organism. For further details of generating bispecific antibodies see, for example, Suresh et al., Methods in Enzvmology, 121:210 (1986).
According to another approach described in WO 96/27011, the interface between a pair of antibody molecules can be engineered to maximize the percentage of heterodimers which are recovered from recombinant cell culture. The preferred interface comprises at least a part of the CH3 region of an antibody constant domain. In this method, one or more small amino acid side chains from the interface of the first antibody molecule are replaced with larger side chains (e.g. tyrosine or tryptophan). Compensatory "cavities" of identical or similar size to the large side chain(s) are created on the interface of the second antibody molecule by replacing large amino acid side chains with smaller ones (e.g. alanine or threonine). This provides a mechanism for increasing the yield of the heterodimer over other unwanted end-products such as homodimers.
Bispecific antibodies can be prepared as full length antibodies or antibody fragments (e.g. F(ab')2 bispecific antibodies). Techniques for generating bispecific antibodies from antibody fragments have been described in the literature. For example, bispecific antibodies can be prepared using chemical linkage. Brennan et al., Science 229:81 (1985) describe a procedure wherein intact antibodies are proteolytically cleaved to generate F(ab')2 fragments. These fragments are reduced in the presence of the dithiol complexing agent sodium arsenite to stabilize vicinal dithiols and prevent intermolecular disulfide formation. The Fab' fragments generated are then converted to thionitrobenzoate (TNB) derivatives. One of the Fab'-TNB derivatives is then reconverted to the Fab'-tbiol by reduction with mercaptoethylamine and is mixed with an equimolar amount of the other Fab'-TNB derivative to form the bispecific antibody. The bispecific antibodies produced can be used as agents for the selective immobilization of enzymes. Additionally, Fab' fragments can be directly recovered from E. coli and chemically coupled to form bispecific antibodies. Shalaby et al., J. Exp. Med. 175:217-225 (1992) describe the production of a fully humanized bispecific antibody F(ab')2 molecule. Each Fab' fragment was separately secreted from E. coli and subjected to directed chemical coupling in vitro to form the bispecific antibody. The bispecific antibody thus formed was able to bind to cells overexpressing the ErbB2 receptor and normal human T cells, as well as trigger the lytic activity of human cytotoxic lymphocytes against human breast tumor targets.
Various techniques for making and isolating bispecific antibody fragments directly from recombinant cell culture have also been described. For example, bispecific antibodies have been produced using leucine zippers. Kostelny et al., J. Immunol. 148(5): 1547-1553 (1992). The leucine zipper peptides from the Fos and Jun proteins were linked to the Fab' portions of two different antibodies by gene fusion. The antibody homodimers were reduced at the hinge region to form monomers and then re-oxidized to form the antibody heterodimers. This method can also be utilized for the production of antibody homodimers. The "diabody" technology described by Hollinger et al., Proc. Natl. Acad. Sci. USA 90:6444-6448 (1993) has provided an alternative mechanism for making bispecific antibody fragments. The fragments comprise a heavy-chain variable domain (VH) connected to a light-chain variable domain (VL) by a linker which is too short to allow pairing between the two domains on the same chain. Accordingly, the VH and VL domains of one fragment are forced to pair with the complementary VL and VH domains of another fragment, thereby forming two antigen-binding sites. Another strategy for making bispecific antibody fragments by the use of single-chain Fv (sFv) dimers has also been reported. See, Gruber et al., J. Immunol. 152:5368 (1994).
Antibodies with more than two valencies are contemplated. For example, trispecific antibodies can be prepared. Tutt et al, J. Immunol. 147:60 (1991).
Exemplary bispecific antibodies can bind to two different epitopes, at least one of which originates in the protein antigen of the invention. Alternatively, an anti-antigenic arm of an immunoglobulin molecule can be combined with an arm which binds to a triggering molecule on a leukocyte such as a T-cell receptor molecule (e.g. CD2, CD3, CD28, or B7), or Fc receptors for IgG (Fc R), such as Fc RI (CD64), Fc RII (CD32) and Fc RIII (CD 16) so as to focus cellular defense mechanisms to the cell expressing the particular antigen. Bispecific antibodies can also be used to direct cytotoxic agents to cells which express a particular antigen. These antibodies possess an antigen-binding arm and an arm which binds a cytotoxic agent or a radionuclide chelator, such as EOTUBE, DPT A, DOT A, or TETA. Another bispecific antibody of interest binds the protein antigen described herein and further binds tissue factor (TF).
Heteroconjugate Antibodies
Heteroconjugate antibodies are also within the scope of the present invention. Heteroconjugate antibodies are composed of two covalently joined antibodies. Such antibodies have, for example, been proposed to target immune system cells to unwanted cells (U.S. Patent No. 4,676,980), and for treatment of HIV infection (WO 91/00360; WO 92/200373; EP 03089). It is contemplated that the antibodies can be prepared in vitro using known methods in synthetic protein chemistry, including those involving crosslinking agents. For example, immunotoxins can be constructed using a disulfide exchange reaction or by forming a thioether bond. Examples of suitable reagents for this purpose include iminothiolate and methyl-4-mercaptobutyrimidate and those disclosed, for example, in U.S. Patent No. 4,676,980.
Effector Function Engineering
It can be desirable to modify the antibody of the invention with respect to effector function, so as to enhance, e.g., the effectiveness of the antibody in treating cancer. For example, cysteine residue(s) can be introduced into the Fc region, thereby allowing interchain disulfide bond formation in this region. The homodimeric antibody thus generated can have improved intemalization capability and/or increased complement-mediated cell killing and antibody-dependent cellular cytotoxicity (ADCC). See Caron et al., J. Exp Med., 176: 1191- 1195 (1992) and Shopes, J. Immunol., 148: 2918-2922 (1992). Homodimeric antibodies with enhanced anti-tumor activity can also be prepared using heterobifunctional cross-linkers as described in Wolff et al. Cancer Research, 53: 2560-2565 (1993). Alternatively, an antibody can be engineered that has dual Fc regions and can thereby have enhanced complement lysis and ADCC capabilities. See Stevenson et al., Anti-Cancer Drug Design, 3: 219-230 (1989). Immunoconjugates
The invention also pertains to immunoconjugates comprising an antibody conjugated to a cytotoxic agent such as a chemotherapeutic agent, toxin (e.g., an enzymatically active toxin of bacterial, fungal, plant, or animal origin, or fragments thereof), or a radioactive isotope (i.e., a radioconjugate).
Chemotherapeutic agents useful in the generation of such immunoconjugates have been described above. Enzymatically active toxins and fragments thereof that can be used include diphtheria A chain, nonbinding active fragments of diphtheria toxin, exotoxin A chain (from Pseudomonas aeruginosa), ricin A chain, abrin A chain, modeccin A chain, alpha-sarcin, Aleurites fordii proteins, dianthin proteins, Phytolaca americana proteins (PAPI, PAPII, and PAP-S), momordica charantia inhibitor, curcin, crotin, sapaonaria officinalis inhibitor, gelonin, mitogellin, restrictocin, phenomycin, enomycin, and the tricothecenes. A variety of radionuclides are available for the production of radioconjugated antibodies. Examples include 12Bi, 1311, 131In, 90Y, and 186Re. Conjugates of the antibody and cytotoxic agent are made using a variety of bifunctional protein-coupling agents such as N-succinimidyl-3-(2-pyridyldithiol) propionate (SPDP), iminothiolane (IT), bifunctional derivatives of imidoesters (such as dimethyl adipimidate HCL), active esters (such as disuccinimidyl suberate), aldehydes (such as glutareldehyde), bis- azido compounds (such as bis (p-azidobenzoyl) hexanediamine), bis-diazonium derivatives (such as bis-(p-diazoniumbenzoyl)-ethylenediamine), diisocyanates (such as tolyene 2,6- diisocyanate), and bis-active fluorine compounds (such as l,5-difluoro-2,4-dinitrobenzene). For example, a ricin immunotoxin can be prepared as described in Vitetta et al, Science, 238: 1098 (1987). Carbon- 14-labeled l-isothiocyanatobenzyl-3-methyldiethylene triaminepentaacetic acid (MX-DTPA) is an exemplary chelating agent for conjugation of radionucleotide to the antibody. See WO94/11026.
In another embodiment, the antibody can be conjugated to a "receptor" (such streptavidin) for utilization in tumor pretargeting wherein the antibody-receptor conjugate is administered to the patient, followed by removal of unbound conjugate from the circulation using a clearing agent and then administration of a "ligand" (e.g., avidin) that is in turn conjugated to a cytotoxic agent. NOVX Recombinant Expression Vectors and Host Cells
Another aspect of the invention pertains to vectors, preferably expression vectors, containing a nucleic acid encoding a NOVX protein, or derivatives, fragments, analogs or homologs thereof. As used herein, the term "vector" refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a "plasmid", which refers to a circular double stranded DNA loop into which additional DNA segments can be ligated. Another type of vector is a viral vector, wherein additional DNA segments can be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors (e.g., non-episomal mammalian vectors) are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively-linked. Such vectors are referred to herein as "expression vectors". In general, expression vectors of utility in recombinant DNA techniques are often in the form of plasmids. In the present specification, "plasmid" and "vector" can be used interchangeably as the plasmid is the most commonly used form of vector. However, the invention is intended to include such other forms of expression vectors, such as viral vectors (e.g., replication defective retro viruses, adenoviruses and adeno-associated viruses), which serve equivalent functions. The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, that is operatively-linked to the nucleic acid sequence to be expressed. Within a recombinant expression vector, "operably-linked" is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner that allows for expression of the nucleotide sequence (e.g., in an in vitro transcription/translation system or in a host cell when the vector is introduced into the host cell).
The term "regulatory sequence" is intended to includes promoters, enhancers and other expression control elements (e.g., polyadenylation signals). Such regulatory sequences are described, for example, in Goeddel, GENE EXPRESSION TECHNOLOGY: METHODS IN ENZYMOLOGY 185, Academic Press, San Diego, Calif. (1990). Regulatory sequences include those that direct constitutive expression of a nucleotide sequence in many types of host cell and those that direct expression of the nucleotide sequence only in certain host cells (e.g., tissue-specific regulatory sequences). It will be appreciated by those skilled in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce proteins or peptides, including fusion proteins or peptides, encoded by nucleic acids as described herein (e.g., NOVX proteins, mutant forms of NOVX proteins, fusion proteins, etc.).
The recombinant expression vectors of the invention can be designed for expression of NOVX proteins in prokaryotic or eukaryotic cells. For example, NOVX proteins can be expressed in bacterial cells such as Escherichia coli, insect cells (using baculovirus expression vectors) yeast cells or mammalian cells. Suitable host cells are discussed further in Goeddel, GENE EXPRESSION TECHNOLOGY: METHODS IN ENZYMOLOGY 185, Academic Press, San Diego, Calif. (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and T7 polymerase. Expression of proteins in prokaryotes is most often carried out in Escherichia coli with vectors containing constitutive or inducible promoters directing the expression of either fusion or non-fusion proteins. Fusion vectors add a number of amino acids to a protein encoded therein, usually to the amino terminus of the recombinant protein. Such fusion vectors typically serve three purposes: (i) to increase expression of recombinant protein; (ii) to increase the solubility of the recombinant protein; and (Hi) to aid in the purification of the recombinant protein by acting as a ligand in affinity purification. Often, in fusion expression vectors, a proteolytic cleavage site is introduced at the junction of the fusion moiety and the recombinant protein to enable separation of the recombinant protein from the fusion moiety subsequent to purification of the fusion protein. Such enzymes, and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase. Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; Smith and Johnson, 1988. Gene 67: 31-40), pMAL (New England Biolabs, Beverly, Mass.) and pRIT5 (Pharmacia, Piscataway, N.J.) that fuse glutathione S-transferase (GST), maltose E binding protein, or protein A, respectively, to the target recombinant protein. Examples of suitable inducible non-fusion E. coli expression vectors include pTrc
(Amrann et al, (1988) Gene 69:301-315) and pET lid (Srudier et al, GENE EXPRESSION TECHNOLOGY: METHODS IN ENZYMOLOGY 185, Academic Press, San Diego, Calif. (1990) 60-89). One strategy to maximize recombinant protein expression in E. coli is to express the protein in a host bacteria with an impaired capacity to proteolytically cleave the recombinant protein. See, e.g., Gottesman, GΕNΕ EXPRESSION TECHNOLOGY: METHODS IN ENZYMOLOGY 185, Academic Press, San Diego, Calif. (1990) 119-128. Another strategy is to alter the nucleic acid sequence of the nucleic acid to be inserted into an expression vector so that the individual codons for each amino acid are those preferentially utilized in E. coli (see, e.g., Wada, et al, 1992. Nucl. Acids Res. 20: 2111-2118). Such alteration of nucleic acid sequences of the invention can be carried out by standard DNA synthesis techniques.
In another embodiment, the NOVX expression vector is a yeast expression vector. Examples of vectors for expression in yeast Saccharomyces cerivisae include pYepSecl (Baldari, et al, 1987. EMBO J. 6: 229-234), pMFa (Kurjan and Herskowitz, 1982. Cell 30: 933-943), pJRY88 (Schultz et al, 1987. Gene 54: 113-123), pYES2 (Invifrogen Corporation, San Diego, Calif.), and picZ (InVitrogen Corp, San Diego, Calif).
Alternatively, NOVX can be expressed in insect cells using baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells (e.g., SF9 cells) include the pAc series (Smith, et al, 1983. Mol. Cell. Biol. 3: 2156-2165) and the pVL series (Lucklow and Summers, 1989. Virology 170: 31-39).
In yet another embodiment, a nucleic acid of the invention is expressed in mammalian cells using a mammalian expression vector. Examples of mammalian expression vectors include pCDM8 (Seed, 1987. Nature 329: 840) and pMT2PC (Kaufman, et al, 1987. EMBO J. 6: 187-195). When used in mammalian cells, the expression vector's control functions are often provided by viral regulatory elements. For example, commonly used promoters are derived from polyoma, adenoviras 2, cytomegaloviras, and simian virus 40. For other suitable expression systems for both prokaryotic and eukaryotic cells see, e.g., Chapters 16 and 17 of Sambrook, et al, MOLECULAR CLONLNG: A LABORATORY MANUAL. 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989. hi another embodiment, the recombinant mammalian expression vector is capable of directing expression of the nucleic acid preferentially in a particular cell type (e.g., tissue-specific regulatory elements are used to express the nucleic acid). Tissue-specific regulatory elements are known in the art. Non-limiting examples of suitable tissue-specific promoters include the albumin promoter (liver-specific; Pinkert, et al, 1987. Genes Dev. 1: 268-277), lymphoid-specific promoters (Calame and Eaton, 1988. Adv. Immunol. 43: 235-275), in particular promoters of T cell receptors (Winoto and Baltimore, 1989. EMBO J. 8: 729-733) and immunoglobulins (Banerji, et al, 1983. Cell 33: 729-740; Queen and Baltimore, 1983. Cell 33: 741-748), neuron-specific promoters (e.g., the neurofilament promoter; Byrne and Ruddle, 1989. Proc. Natl. Acad. Sci. USA 86: 5473-5477), pancreas-specific promoters (Edlund, et al, 1985. Science 230: 912-916), and mammary gland-specific promoters (e.g., milk whey promoter; U.S. Pat. No. 4,873,316 and European Application Publication No. 264,166). Developmentally-regulated promoters are also encompassed, e.g., the murine hox promoters (Kessel and Grass, 1990. Science 249: 374-379) and the α-fetoprotein promoter (Campes and Tilghman, 1989. Genes Dev. 3: 537-546).
The invention further provides a recombinant expression vector comprising a DNA molecule of the invention cloned into the expression vector in an antisense orientation. That is, the DNA molecule is operatively-linked to a regulatory sequence in a manner that allows for expression (by transcription of the DNA molecule) of an RNA molecule that is antisense to NOVX mRNA. Regulatory sequences operatively linked to a nucleic acid cloned in the antisense orientation can be chosen that direct the continuous expression of the antisense RNA molecule in a variety of cell types, for instance viral promoters and/or enhancers, or regulatory sequences can be chosen that direct constitutive, tissue specific or cell type specific expression of antisense RNA. The antisense expression vector can be in the form of a recombinant plasmid, phagemid or attenuated virus in which antisense nucleic acids are produced under the control of a high efficiency regulatory region, the activity of which can be determined by the cell type into which the vector is introduced. For a discussion of the regulation of gene expression using antisense genes see, e.g., Weintraub, et al, "Antisense RNA as a molecular tool for genetic analysis," Reviews-Trends in Genetics, Vol. 1(1) 1986.
Another aspect of the invention pertains to host cells into which a recombinant expression vector of the invention has been introduced. The terms "host cell" and "recombinant host cell" are used interchangeably herein. It is understood that such terms refer not only to the particular subject cell but also to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein. A host cell can be any prokaryotic or eukaryotic cell. For example, NOVX protein can be expressed in bacterial cells such as E. coli, insect cells, yeast or mammalian cells (such as human, Chinese hamster ovary cells (CHO) or COS cells). Other suitable host cells are known to those skilled in the art. Vector DNA can be introduced into prokaryotic or eukaryotic cells via conventional transformation or transfection techniques. As used herein, the terms "transformation" and "fransfection" are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid (e.g., DNA) into a host cell, including calcium phosphate or calcium chloride co-precipitation, DEAE-dextran-mediated transfection, hpofection, or elecfroporation. Suitable methods for transforming or transfecting host cells can be found in Sambrook, et al. (MOLECULAR CLONING: A LABORATORY MANUAL. 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989), and other laboratory manuals. For stable transfection of mammalian cells, it is known that, depending upon the expression vector and transfection technique used, only a small fraction of cells may integrate the foreign DNA into their genome. In order to identify and select these integrants, a gene that encodes a selectable marker (e.g., resistance to antibiotics) is generally introduced into the host cells along with the gene of interest. Various selectable markers include those that confer resistance to drags, such as G418, hygromycin and methotrexate. Nucleic acid encoding a selectable marker can be introduced into a host cell on the same vector as that encoding NOVX or can be introduced on a separate vector. Cells stably transfected with the introduced nucleic acid can be identified by drag selection (e.g., cells that have incorporated the selectable marker gene will survive, while the other cells die). A host cell of the invention, such as a prokaryotic or eukaryotic host cell in culture, can be used to produce (i.e., express) NOVX protein. Accordingly, the invention further provides methods for producing NOVX protein using the host cells of the invention. In one embodiment, the method comprises culturing the host cell of invention (into which a recombinant expression vector encoding NOVX protein has been introduced) in a suitable medium such that NOVX protein is produced. In another embodiment, the method further comprises isolating NOVX protein from the medium or the host cell.
Transgenic NOVX Animals
The host cells of the invention can also be used to produce non-human transgenic animals. For example, in one embodiment, a host cell of the invention is a fertilized oocyte or an embryonic stem cell into which NOVX protein-coding sequences have been introduced. Such host cells can then be used to create non-human transgenic animals in which exogenous NOVX sequences have been introduced into their genome or homologous recombinant animals in which endogenous NOVX sequences have been altered. Such animals are useful for studying the function and/or activity of NOVX protein and for identifying and/or evaluating modulators of NOVX protein activity. As used herein, a "transgenic animal" is a non-human animal, preferably a mammal, more preferably a rodent such as a rat or mouse, in which one or more of the cells of the animal includes a fransgene. Other examples of transgenic animals include non-human primates, sheep, dogs, cows, goats, chickens, amphibians, etc. A fransgene is exogenous DNA that is integrated into the genome of a cell from which a transgenic animal develops and that remains in the genome of the mature animal, thereby directing the expression of an encoded gene product in one or more cell types or tissues of the transgenic animal. As used herein, a "homologous recombinant animal" is a non-human animal, preferably a mammal, more preferably a mouse, in which an endogenous NOVX gene has been altered by homologous recombination between the endogenous gene and an exogenous DNA molecule introduced into a cell of the animal, e.g., an embryonic cell of the animal, prior to development of the animal. A transgenic animal of the invention can be created by introducing NOVX-encoding nucleic acid into the male pronuclei of a fertilized oocyte (e.g., by microinjection, retroviral infection) and allowing the oocyte to develop in a pseudopregnant female foster animal. Sequences including SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 can be introduced as a fransgene into the genome of a non-human animal. Alternatively, a non-human homologue of the huma NOVX gene, such as a mouse NOVX gene, can be isolated based on hybridization to the huma NOVX cDNA (described further supra) and used as a fransgene. Intronic sequences and polyadenylation signals can also be included in the fransgene to increase the efficiency of expression of the fransgene. A tissue-specific regulatory sequence(s) can be operably-linked to the NOVX fransgene to direct expression of NOVX protein to particular cells. Methods for generating transgenic animals via embryo manipulation and microinjection, particularly ammals such as mice, have become conventional in the art and are described, for example, in U.S. Patent Nos. 4,736,866; 4,870,009; and 4,873,191; and Hogan, 1986. In: MANIPULATING THE MOUSE EMBRYO, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. Similar methods are used for production of other fransgenic animals. A transgenic founder animal can be identified based upon the presence of the NOVX fransgene in its genome and/or expression of NOVX mRNA in tissues or cells of the animals. A fransgenic founder animal can then be used to breed additional animals carrying the fransgene. Moreover, fransgenic animals carrying a fransgene-encoding NOVX protein can further be bred to other fransgenic animals carrying other transgenes.
To create a homologous recombinant animal, a vector is prepared which contains at least a portion of a NOVX gene into which a deletion, addition or substitution has been introduced to thereby alter, e.g., functionally disrupt, the NOVX gene. The NOVX gene can be a human gene (e.g., the DNA of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23), but more preferably, is a non-human homologue of a huma NOVX gene. For example, a mouse homologue of huma NOVX gene of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 can be used to construct a homologous recombination vector suitable for altering an endogenous NOVX gene in the mouse genome. In one embodiment, the vector is designed such that, upon homologous recombination, the endogenous NOVX gene is functionally disrupted (i.e., no longer encodes a functional protein; also referred to as a "knock out" vector).
Alternatively, the vector can be designed such that, upon homologous recombination, the endogenous NOVX gene is mutated or otherwise altered but still encodes functional protein (e.g., the upstream regulatory region can be altered to thereby alter the expression of the endogenous NOVX protein). In the homologous recombination vector, the altered portion of the NOVX gene is flanked at its 5'- and 3'-termini by additional nucleic acid of the NOVX gene to allow for homologous recombination to occur between the exogenous NOVX gene carried by the vector and an endogenous NOVX gene in an embryonic stem cell. The additional flanking NOVX nucleic acid is of sufficient length for successful homologous recombination with the endogenous gene. Typically, several kilobases of flanking DNA (both at the 5'- and 3'-termini) are included in the vector. See, e.g., Thomas, et al, 1987. Cell 51: 503 for a description of homologous recombination vectors. The vector is ten introduced into an embryonic stem cell line (e.g., by elecfroporation) and cells in which the introduced NOVX gene has homologously-recombined with the endogenous NOVX gene are selected. See, e.g., U, et al, 1992. Cell 69: 915.
The selected cells are then injected into a blastocyst of an animal (e.g., a mouse) to form aggregation chimeras. See, e.g., Bradley, 1987. fri: TERATOCARCINOMAS AND EMBRYONIC STEM CELLS: A PRACTICAL APPROACH, Robertson, ed. LRL, Oxford, pp. 113-152. A chimeric embryo can then be implanted into a suitable pseudopregnant female foster animal and the embryo brought to term. Progeny harboring the homologously-recombined DNA in their germ cells can be used to breed animals in which all cells of the animal contain the homologously-recombined DNA by germline transmission of the fransgene. Methods for constructing homologous recombination vectors and homologous recombinant animals are described further in Bradley, 1991. Curr. Opin. Biotechnol. 2: 823-829; PCT International Publication Nos.: WO 90/11354; WO 91/01140; WO 92/0968; and WO 93/04169.
In another embodiment, fransgenic non-humans animals can be produced that contain selected systems that allow for regulated expression of the fransgene. One example of such a system is the cre/loxP recombinase system of bacteriophage PI. For a description of the cre/loxP recombinase system, See, e.g., Lakso, et al, 1992. Proc. Natl. Acad. Sci. USA 89: 6232-6236. Another example of a recombinase system is the FLP recombinase system of Saccharomyces cerevisiae. See, O' 'Gorman, et al, 1991. Science 251:1351-1355. Ifa cre/loxP recombinase system is used to regulate expression of the fransgene, animals containing transgenes encoding both the Cre recombinase and a selected protein are required. Such animals can be provided through the construction of "double" fransgenic animals, e.g., by mating two transgenic animals, one containing a fransgene encoding a selected protein and the other containing a fransgene encoding a recombinase. Clones of the non-human transgenic animals described herein can also be produced according to the methods described in Wilmut, et al, 1997. Nature 385: 810-813. In brief, a cell (e.g., a somatic cell) from the transgenic animal can be isolated and induced to exit the growth cycle and enter G0 phase. The quiescent cell can then be fused, e.g., through the use of electrical pulses, to an enucleated oocyte from an animal of the same species from which the quiescent cell is isolated. The reconstracted oocyte is then cultured such that it develops to morula or blastocyte and then transferred to pseudopregnant female foster animal. The offspring borne of this female foster animal will be a clone of the animal from which the cell (e.g., the somatic cell) is isolated.
Pharmaceutical Compositions
The NOVX nucleic acid molecules, NOVX proteins, and anti-NOVX antibodies (also referred to herein as "active compounds") of the invention, and derivatives, fragments, analogs and homologs thereof, can be incorporated into pharmaceutical compositions suitable for administration. Such compositions typically comprise the nucleic acid molecule, protein, or antibody and a pharmaceutically acceptable carrier. As used herein, "pharmaceutically acceptable carrier" is intended to include any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration. Suitable carriers are described in the most recent edition of Remington's Pharmaceutical Sciences, a standard reference text in the field, which is incorporated herein by reference. Preferred examples of such carriers or diluents include, but are not limited to, water, saline, finger's solutions, dexfrose solution, and 5% human serum albumin. Liposomes and non-aqueous vehicles such as fixed oils may also be used. The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active compound, use thereof in the compositions is contemplated. Supplementary active compounds can also be incorporated into the compositions.
The antibodies disclosed herein can also be formulated as innmmoiiposom.es. Liposomes containing the antibody are prepared by methods known in the art, such as described in Epstein et al., Proc. Natl. Acad. Sci. USA, 82: 3688 (1985); Hwang et al., Proc. Natl Acad. Sci. USA, 77: 4030 (1980); and U.S. Pat. Nos. 4,485,045 and 4,544,545. Liposomes with enhanced circulation time are disclosed in U.S. Patent No. 5,013,556. Particularly useful liposomes can be generated by the reverse-phase evaporation method with a lipid composition comprising phosphatidylcholine, cholesterol, and PEG- derivatized phosphatidylethanolamine (PEG-PE). Liposomes are extruded through filters of defined pore size to yield liposomes with the desired diameter. Fab' fragments of the antibody of the present invention can be conjugated to the liposomes as described in Martin et al ., J. Biol. Chem., 257: 286-288 (1982) via a disulfide-interchange reaction. A chemotherapeutic agent (such as Doxorabicin) is optionally contained within the liposome. See Gabizon et al., J. National Cancer Inst., 81(19): 1484 (1989).
A pharmaceutical composition of the invention is formulated to be compatible with its intended route of administration. Examples of routes of administration include parenteral, e.g., intravenous, intradermal, subcutaneous, oral (e.g., inhalation), transdermal (i.e., topical), transmucosal, and rectal administration. Solutions or suspensions used for parenteral, intradermal, or subcutaneous application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetefraacetic acid (EDTA); buffers such as acetates, citrates or phosphates, and agents for the adjustment of tonicity such as sodium chloride or dexfrose. The pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide. The parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic.
Pharmaceutical compositions suitable for injectable use include sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. For intravenous administration, suitable carriers include physiological saline, bacteriostatic water, Cremophor EL™ (BASF, Parsippany, N.J.) or phosphate buffered saline (PBS). In all cases, the composition must be sterile and should be fluid to the extent that easy syringeability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. Prevention of the action of microorganisms can be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars, polyalcohols such as manitol, sorbitol, sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate and gelatin.
Sterile injectable solutions can be prepared by incorporating the active compound (e.g., a NOVX protein or anti-NOVX antibody) in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the active compound into a sterile vehicle that contains a basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, methods of preparation are vacuum drying and freeze-drying that yields a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.
Oral compositions generally include an inert diluent or an edible carrier. They can be enclosed in gelatin capsules or compressed into tablets. For the purpose of oral therapeutic administration, the active compound can be incorporated with excipients and used in the form of tablets, froches, or capsules. Oral compositions can also be prepared using a fluid carrier for use as a mouthwash, wherein the compound in the fluid carrier is applied orally and swished and expectorated or swallowed. Pharmaceutically compatible binding agents, and/or adjuvant materials can be included as part of the composition. The tablets, pills, capsules, troches and the like can contain any of the following ingredients, or compounds of a similar nature: a binder such as microcrystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose, a disintegrating agent such as alginic acid, Primogel, or corn starch; a lubricant such as magnesium stearate or Sterotes; a glidant such as colloidal silicon dioxide; a sweetening agent such as sucrose or saccharin; or a flavoring agent such as peppermint, methyl salicylate, or orange flavoring.
For administration by inhalation, the compounds are delivered in the form of an aerosol spray from pressured container or dispenser which contains a suitable propellant, e.g., a gas such as carbon dioxide, or a nebulizer.
Systemic administration can also be by fransmucosal or transdermal means. For fransmucosal or transdermal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art, and include, for example, for fransmucosal administration, detergents, bile salts, and fusidic acid derivatives. Transmucosal administration can be accomplished through the use of nasal sprays or suppositories. For transdermal administration, the active compounds are formulated into ointments, salves, gels, or creams as generally known in the art.
The compounds can also be prepared in the form of suppositories (e.g., with conventional suppository bases such as cocoa butter and other glycerides) or retention enemas for rectal delivery.
In one embodiment, the active compounds are prepared with carriers that will protect the compound against rapid elimination from the body, such as a controlled release formulation, including implants and microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Methods for preparation of such formulations will be apparent to those skilled in the art. The materials can also be obtained commercially from Alza Corporation and Nova Pharmaceuticals, Inc. Liposomal suspensions (including liposomes targeted to infected cells with monoclonal antibodies to viral antigens) can also be used as pharmaceutically acceptable carriers. These can be prepared according to methods known to those skilled in the art, for example, as described in U.S. Patent No. 4,522,811.
It is especially advantageous to formulate oral or parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subject to be freated; each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. The specification for the dosage unit forms of the invention are dictated by and directly dependent on the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and the limitations inherent in the art of compounding such an active compound for the freatment of individuals.
The nucleic acid molecules of the invention can be inserted into vectors and used as gene therapy vectors. Gene therapy vectors can be delivered to a subject by, for example, intravenous injection, local administration (see, e.g., U.S. Patent No. 5,328,470) or by stereotactic injection (see, e.g., Chen, et al, 1994. Proc. Natl. Acad. Sci. USA 91: 3054-3057). The pharmaceutical preparation of the gene therapy vector can include the gene therapy vector in an acceptable diluent, or can comprise a slow release matrix in which the gene delivery vehicle is imbedded. Alternatively, where the complete gene delivery vector can be produced intact from recombinant cells, e.g., retroviral vectors, the pharmaceutical preparation can include one or more cells that produce the gene delivery system.
Antibodies specifically binding a protein of the invention, as well as other molecules identified by the screening assays disclosed herein, can be administered for the treatment of various disorders in the form of pharmaceutical compositions. Principles and considerations involved in preparing such compositions, as well as guidance in the choice of components are provided, for example, in Remington : The Science And Practice Of Pharmacy 19th ed. (Alfonso R. Gennaro, et al., editors) Mack Pub. Co., Easton, Pa. : 1995; Drug Absorption Enhancement : Concepts, Possibilities, Limitations, And Trends, Harwood Academic Publishers, Langhorne, Pa., 1994; and Peptide And Protein Drug Delivery (Advances In Parenteral Sciences, Vol. 4), 1991, M. Dekker, New York. If the antigenic protein is infracellular and whole antibodies are used as inhibitors, internalizing antibodies are preferred. However, liposomes can also be used to deliver the antibody, or an antibody fragment, into cells. Where antibody fragments are used, the smallest inhibitory fragment that specifically binds to the binding domain of the target protein is preferred. For example, based upon the variable-region sequences of an antibody, peptide molecules can be designed that retain the ability to bind the target protein sequence. Such peptides can be synthesized chemically and or produced by recombinant DNA technology. See, e.g., Marasco et al, 1993 Proc. Natl. Acad. Sci. USA, 90: 7889-7893. The formulation herein can also contain more than one active compound as necessary for the particular indication being freated, preferably those with complementary activities that do not adversely affect each other. Alternatively, or in addition, the composition can comprise an agent that enhances its function, such as, for example, a cytotoxic agent, cytokine, chemotherapeutic agent, or growth-inhibitory agent. Such molecules are suitably present in combination in amounts that are effective for the purpose intended. The active ingredients can also be entrapped in microcapsules prepared, for example, by coacervation techniques or by interfacial polymerization, for example, hydroxymethylcellulose or gelatin-microcapsules and poly-(methylmethacrylate) microcapsules, respectively, in colloidal drag delivery systems (for example, liposomes, albumin microspheres, microemulsions, nano-particles, and nanocapsules) or in macroemulsions.
The formulations to be used for iv vivo administration must be sterile. This is readily accomplished by filtration through sterile filtration membranes.
Sustained-release preparations can be prepared. Suitable examples of sustained-release preparations include semipermeable matrices of solid hydrophobic polymers containing the antibody, which matrices are in the form of shaped articles, e.g., films, or microcapsules. Examples of sustained-release matrices include polyesters, hydrogels (for example, poly(2- hydroxyethyl-methacrylate), or poly(vinylalcohol)), polylactides (U.S. Pat. No. 3,773,919), copolymers of L-glutamic acid and ethyl-L-glutamate, non-degradable ethylene- vinyl acetate, degradable lactic acid-glycolic acid copolymers such as the LUPRON DEPOT ™ (injectable microspheres composed of lactic acid-glycolic acid copolymer and leuprolide acetate), and poly-D-(-)-3-hydroxybutyric acid. While polymers such as ethylene-vinyl acetate and lactic acid-glycolic acid enable release of molecules for over 100 days, certain hydrogels release proteins for shorter time periods.
The pharmaceutical compositions can be included in a container, pack, or dispenser together with instructions for administration. Screening and Detection Methods
The isolated nucleic acid molecules of the invention can be used to express NOVX protein (e.g., via a recombinant expression vector in a host cell in gene therapy applications), to detect NOVX mRNA (e.g., in a biological sample) or a genetic lesion in a NOVX gene, and to modulate NOVX activity, as described further, below. In addition, the NOVX proteins can be used to screen drugs or compounds that modulate the NOVX protein activity or expression as well as to treat disorders characterized by insufficient or excessive production of NOVX protein or production of NOVX protein forms that have decreased or aberrant activity compared to NOVX wild-type protein. In addition, the anti-NOVX antibodies of the invention can be used to detect and isolate NOVX proteins and modulate NOVX activity. For example, NOVX activity includes growth and differentiation, antibody production, and tumor growth.
The invention further pertains to novel agents identified by the screening assays described herein and uses thereof for treatments as described, supra.
Screening Assays
The invention provides a method (also referred to herein as a "screening assay") for identifying modulators, i.e., candidate or test compounds or agents (e.g., peptides, peptidomimetics, small molecules or other drugs) that bind to NOVX proteins or have a stimulatory or inhibitory effect on, e.g., NOVX protein expression or NOVX protein activity. The invention also includes compounds identified in the screening assays described herein.
In one embodiment, the invention provides assays for screening candidate or test compounds which bind to or modulate the activity of the membrane-bound form of a NOVX protem or polypeptide or biologically-active portion thereof. The test compounds of the invention can be obtained using any of the numerous approaches in combinatorial library methods known in the art, including: biological libraries; spatially addressable parallel solid phase or solution phase libraries; synthetic library methods requiring deconvolution; the "one-bead one-compound" library method; and synthetic library methods using affinity chromatography selection. The biological library approach is limited to peptide libraries, while the other four approaches are applicable to peptide, non-peptide oligomer or small molecule libraries of compounds. See, e.g., Lam, 1997 '. Anticancer Drug Design 12: 145.
A "small molecule" as used herein, is meant to refer to a composition that has a molecular weight of less than about 5 kD and most preferably less than about 4 kD. Small molecules can be, e.g., nucleic acids, peptides, polypeptides, peptidomimetics, carbohydrates, lipids or other organic or inorganic molecules. Libraries of chemical and/or biological mixtures, such as fungal, bacterial, or algal extracts, are known in the art and can be screened with any of the assays of the invention.
Examples of methods for the synthesis of molecular libraries can be found in the art, for example in: DeWitt, et al, 1993. Proc. Natl. Acad. Sci. U.S.A. 90: 6909; Erb, et al, 1994. Proc. Natl. Acad. Sci. U.S.A. 91: 11422; Zuckermann, et al, 1994. J Med. Chem. 37: 2678; Cho, et al, 1993. Science 261: 1303; Carrell, et al, 1994. Angew. Chem. Int. Ed. Engl. 33: 2059; Carell, et al, 1994. Angew. Chem. Int. Ed. Engl. 33: 2061; and Gallop, et al, 1994. J. Med. Chem. 37: 1233. Libraries of compounds may be presented in solution (e.g., Houghten, 1992.
Biotechniques 13: 412-421), or on beads (Lam, 1991. Nature 354: 82-84), on chips (Fodor, 1993. Nature 364: 555-556), bacteria (Ladner, U.S. Patent No. 5,223,409), spores (Ladner, U.S. Patent 5,233,409), plasmids (Cull, et al, 1992. Proc. Natl. Acad. Sci. USA 89: 1865-1869) or on phage (Scott and Smith, 1990. Science 249: 386-390; Devlin, 1990. Science 249: 404-406; Cwirla, et al, 1990. Proc. Natl. Acad. Sci. U.S.A. 87: 6378-6382; Felici, 1991. J. Mol. Biol. 222: 301-310; Ladner, U.S. Patent No. 5,233,409.).
In one embodiment, an assay is a cell-based assay in which a cell which expresses a membrane-bound form of NOVX protein, or a biologically-active portion thereof, on the cell surface is contacted with a test compound and the ability of the test compound to bind to a NOVX protein determined. The cell, for example, can be of mammalian origin or a yeast cell. Determining the ability of the test compound to bind to the NOVX protein can be accomplished, for example, by coupling the test compound with a radioisotope or enzymatic label such that binding of the test compound to the NOVX protein or biologically-active portion thereof can be determined by detecting the labeled compound in a complex. For example, test compounds can be labeled with 1251, 35S, 14C, or 3H, either directly or indirectly, and the radioisotope detected by direct counting of radioemission or by scintillation counting. Alternatively, test compounds can be enzymatically-labeled with, for example, horseradish peroxidase, alkaline phosphatase, or luciferase, and the enzymatic label detected by determination of conversion of an appropriate substrate to product. In one embodiment, the assay comprises contacting a cell which expresses a membrane-bound form of NOVX protein, or a biologically-active portion thereof, on the cell surface with a known compound which binds NOVX to form an assay mixture, contacting the assay mixture with a test compound, and determining the ability of the test compound to interact with a NOVX protein, wherein determining the ability of the test compound to interact with a NOVX protein comprises determining the ability of the test compound to preferentially bind to NOVX protein or a biologically-active portion thereof as compared to the known compound.
In another embodiment, an assay is a cell-based assay comprising contacting a cell expressing a membrane-bound form of NOVX protem, or a biologically-active portion thereof, on the cell surface with a test compound and determining the ability of the test compound to modulate (e.g., stimulate or inhibit) the activity of the NOVX protem or biologically-active portion thereof. Determining the ability of the test compound to modulate the activity of NOVX or a biologically-active portion thereof can be accomplished, for example, by determining the ability of the NOVX protein to bind to or interact with a NOVX target molecule. As used herein, a "target molecule" is a molecule with which a NOVX protein binds or interacts in nature, for example, a molecule on the surface of a cell which expresses a NOVX interacting protein, a molecule on the surface of a second cell, a molecule in the extracellular milieu, a molecule associated with the internal surface of a cell membrane or a cytoplasmic molecule. A NOVX target molecule can be a non-NOVX molecule or a NOVX protein or polypeptide of the invention In one embodiment, a NOVX target molecule is a component of a signal fransduction pathway that facilitates fransduction of an extracellular signal (e.g. a signal generated by binding of a compound to a membrane-bound NOVX molecule) through the cell membrane and into the cell. The target, for example, can be a second intercellular protein that has catalytic activity or a protein that facilitates the association of downstream signaling molecules with NOVX.
Determining the ability of the NOVX protein to bind to or interact with a NOVX target molecule can be accomplished by one of the methods described above for determining direct binding. In one embodiment, determining the ability of the NOVX protein to bind to or interact with a NOVX target molecule can be accomplished by determining the activity of the target molecule. For example, the activity of the target molecule can be determined by detecting induction of a cellular second messenger of the target (i.e. infracellular Ca2+, diacylglycerol, LP3, etc.), detecting catalytic/enzymatic activity of the target an appropriate substrate, detecting the induction of a reporter gene (comprising a NOVX-responsive regulatory element operatively linked to a nucleic acid encoding a detectable marker, e.g., luciferase), or detecting a cellular response, for example, cell survival, cellular differentiation, or cell proliferation. In yet another embodiment, an assay of the invention is a cell-free assay comprising contacting a NOVX protein or biologically-active portion thereof with a test compound and determining the ability of the test compound to bind to the NOVX protein or biologically- active portion thereof. Binding of the test compound to the NOVX protein can be determined either directly or indirectly as described above. In one such embodiment, the assay comprises contacting the NOVX protein or biologically-active portion thereof with a known compound which binds NOVX to form an assay mixture, contacting the assay mixture with a test compound, and determining the ability of the test compound to interact with a NOVX protein, wherein determining the ability of the test compound to interact with a NOVX protein comprises determining the ability of the test compound to preferentially bind to NOVX or biologically-active portion thereof as compared to the known compound.
In still another embodiment, an assay is a cell-free assay comprising contacting NOVX protein or biologically-active portion thereof with a test compound and determining the ability of the test compound to modulate (e.g. stimulate or inhibit) the activity of the NOVX protem or biologically-active portion thereof. Determining the ability of the test compound to modulate the activity of NOVX can be accomplished, for example, by determining the ability of the NOVX protein to bind to a NOVX target molecule by one of the methods described above for determining direct binding. In an alternative embodiment, determining the ability of the test compound to modulate the activity of NOVX protein can be accomplished by determining the ability of the NOVX protein further modulate a NOVX target molecule. For example, the catalytic/enzymatic activity of the target molecule on an appropriate substrate can be determined as described above.
In yet another embodiment, the cell-free assay comprises contacting the NOVX protein or biologically-active portion thereof with a known compound which binds NOVX protein to form an assay mixture, contacting the assay mixture with a test compound, and determining the ability of the test compound to interact with a NOVX protein, wherein determining the ability of the test compound to interact with a NOVX protein comprises determining the ability of the NOVX protein to preferentially bind to or modulate the activity of a NOVX target molecule. The cell-free assays of the invention are amenable to use of both the soluble form or the membrane-bound form of NOVX protein. In the case of cell-free assays comprising the membrane-bound form of NOVX protein, it may be desirable to utilize a solubilizing agent such that the membrane-bound form of NOVX protein is maintained in solution. Examples of such solubilizing agents include non-ionic detergents such as n-octylglucoside, n-dodecylglucoside, n-dodecylmaltoside, octanoyl-N-methylglucamide, decanoyl-N-methylglucamide, Triton® X-100, Triton® X-114, Thesit®, Isotridecypoly(ethylene glycol ether)n, N-dodecyl— N,N-dimethyl-3-ammonio-l-propane sulfonate, 3-(3-cholamidopropyl) dhnethylamminiol-1-propane sulfonate (CHAPS), or
3-(3-cholamidopropyl)dimethylamminiol-2-hydroxy-l-propane sulfonate (CHAPSO).
In more than one embodiment of the above assay methods of the invention, it may be desirable to immobilize either NOVX protein or its target molecule to facilitate separation of complexed from uncomplexed forms of one or both of the proteins, as well as to accommodate automation of the assay. Binding of a test compound to NOVX protein, or interaction of NOVX protein with a target molecule in the presence and absence of a candidate compound, can be accomplished in any vessel suitable for containing the reactants. Examples of such vessels include microtiter plates, test tubes, and micro-centrifuge tubes, hi one embodiment, a fusion protein can be provided that adds a domain that allows one or both of the proteins to be bound to a matrix. For example, GST-NO VX fusion proteins or GST-target fusion proteins can be adsorbed onto glutathione sepharose beads (Sigma Chemical, St. Louis, MO) or glutathione derivatized microtiter plates, that are then combined with the test compound or the test compound and either the non-adsorbed target protein or NOVX protein, and the mixture is incubated under conditions conducive to complex formation (e.g., at physiological conditions for salt and pH). Following incubation, the beads or microtiter plate wells are washed to remove any unbound components, the matrix immobilized in the case of beads, complex determined either directly or indirectly, for example, as described, supra. Alternatively, the complexes can be dissociated from the matrix, and the level of NOVX protein binding or activity determined using standard techniques. Other techniques for immobilizing proteins on matrices can also be used in the screening assays of the invention. For example, either the NOVX protein or its target molecule can be immobilized utilizing conjugation of biotin and streptavidin. Biotinylated NOVX protein or target molecules can be prepared from biotin-NHS (N-hydroxy-succinimide) using techniques well-known within the art (e.g., biotinylation kit, Pierce Chemicals, Rockford, 111.), and immobilized in the wells of sfreptavidin-coated 96 well plates (Pierce Chemical). Alternatively, antibodies reactive with NOVX protem or target molecules, but which do not interfere with binding of the NOVX protein to its target molecule, can be derivatized to the wells of the plate, and unbound target or NOVX protein trapped in the wells by antibody conjugation. Methods for detecting such complexes, in addition to those described above for the GST-immobilized complexes, include immunodetection of complexes using antibodies reactive with the NOVX protein or target molecule, as well as enzyme-linked assays that rely on detecting an enzymatic activity associated with the NOVX protein or target molecule.
In another embodiment, modulators of NOVX protein expression are identified in a method wherein a cell is contacted with a candidate compound and the expression of NOVX mRNA or protein in the cell is determined. The level of expression of NOVX mRNA or protein in the presence of the candidate compound is compared to the level of expression of NOVX mRNA or protein in the absence of the candidate compound. The candidate compound can then be identified as a modulator of NOVX mRNA or protein expression based upon this comparison. For example, when expression of NOVX mRNA or protein is greater (i.e., statistically significantly greater) in the presence of the candidate compound than in its absence, the candidate compound is identified as a stimulator of NOVX mRNA or protein expression. Alternatively, when expression of NOVX mRNA or protein is less (statistically significantly less) in the presence of the candidate compound than in its absence, the candidate compound is identified as an inhibitor of NOVX mRNA or protein expression. The level of NOVX mRNA or protein expression in the cells can be determined by methods described herein for detecting NOVX mRNA or protein. In yet another aspect of the invention, the NOVX proteins can be used as "bait proteins" in a two-hybrid assay or three hybrid assay (see, e.g., U.S. Patent No. 5,283,317; Zervos, et al, 1993. Ce// 72: 223-232; Madura, et al, 1993. J. Biol. Chem. 268: 12046-12054; Bartel, et al, 1993. Biotechniques 14: 920-924; Iwabuchi, et al, 1993. Oncogene 8: 1693-1696; and Brent WO 94/10300), to identify other proteins that bind to or interact with NOVX ("NOVX-binding proteins" or "NOVX-bp") and modulate NOVX activity. Such NOVX-binding proteins are also likely to be involved in the propagation of signals by the NOVX proteins as, for example, upstream or downstream elements of the NONX pathway. The two-hybrid system is based on the modular nature of most transcription factors, which consist of separable DNA-binding and activation domains. Briefly, the assay utilizes two different DNA constructs. In one constract, the gene that codes for NOVX is fused to a gene encoding the DNA binding domain of a known transcription factor (e.g., GAL-4). In the other constract, a DNA sequence, from a library of DNA sequences, that encodes an unidentified protein ("prey" or "sample") is fused to a gene that codes for the activation domain of the known transcription factor. If the "bait" and the "prey" proteins are able to interact, in vivo, forming a NOVX-dependent complex, the DNA-binding and activation domains of the transcription factor are brought into close proximity. This proximity allows transcription of a reporter gene (e.g., LacZ) that is operably linked to a transcriptional regulatory site responsive to the transcription factor. Expression of the reporter gene can be detected and cell colonies containing the functional transcription factor can be isolated and used to obtain the cloned gene that encodes the protein which interacts with NOVX.
The invention further pertains to novel agents identified by the aforementioned screening assays and uses thereof for treatments as described herein.
Detection Assays
Portions or fragments of the cDNA sequences identified herein (and the corresponding complete gene sequences) can be used in numerous ways as polynucleotide reagents. By way of example, and not of limitation, these sequences can be used to: (/) identify an individual from a minute biological sample (tissue typing); and (ii) aid in forensic identification of a biological sample. Some of these applications are described in the subsections, below.
Tissue Typing
The NOVX sequences of the invention can be used to identify individuals from minute biological samples. In this technique, an individual's genomic DNA is digested with one or more restriction enzymes, and probed on a Southern blot to yield unique bands for identification. The sequences of the invention are useful as additional DNA markers for RFLP ("restriction fragment length polymorphisms," described in U.S. Patent No. 5,272,057). Furthermore, the sequences of the invention can be used to provide an alternative technique that determines the actual base-by-base DNA sequence of selected portions of an individual's genome. Thus, the NOVX sequences described herein can be used to prepare two PCR primers from the 5'- and 3'-termini of the sequences. These primers can then be used to amplify an individual's DNA and subsequently sequence it.
Panels of corresponding DNA sequences from individuals, prepared in this manner, can provide unique individual identifications, as each individual will have a unique set of such DNA sequences due to allelic differences. The sequences of the invention can be used to obtain such identification sequences from individuals and from tissue. The NOVX sequences of the invention uniquely represent portions of the human genome. Allelic variation occurs to some degree in the coding regions of these sequences, and to a greater degree in the noncoding regions. It is estimated that allelic variation between individual humans occurs with a frequency of about once per each 500 bases. Much of the allelic variation is due to single nucleotide polymorphisms (SNPs), which include restriction fragment length polymorphisms (RFLPs). Each of the sequences described herein can, to some degree, be used as a standard against which DNA from an individual can be compared for identification purposes. Because greater numbers of polymorphisms occur in the noncoding regions, fewer sequences are necessary to differentiate individuals. The noncoding sequences can comfortably provide positive individual identification with a panel of perhaps 10 to 1,000 primers that each yield a noncoding amplified sequence of 100 bases. If predicted coding sequences, such as those in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23 are used, a more appropriate number of primers for positive individual identification would be 500-2,000.
Predictive Medicine The invention also pertains to the field of predictive medicine in which diagnostic assays, prognostic assays, pharmacogenomics, and monitoring clinical trials are used for prognostic (predictive) purposes to thereby treat an individual prophylactically. Accordingly, one aspect of the invention relates to diagnostic assays for determining NOVX protein and/or nucleic acid expression as well as NOVX activity, in the context of a biological sample (e.g., blood, serum, cells, tissue) to thereby determine whether an individual is afflicted with a disease or disorder, or is at risk of developing a disorder, associated with aberrant NOVX expression or activity. Disorders associated with aberrant NOVX expression of activity include, for example, disorders characterized by aberrant cell proliferation, differentiation and migration, e.g. cancer, angiogenesis, atherosclerosis and obesity, neurological disorders, e.g. stroke, Pendred syndrome, multiple sclerosis and Alzheimer's disease, keratinocyte defects, e.g. lesional psoriatic skin, ischemic disorders, e.g. diabetic retinopathy, hepatic disorders, e.g. cirrhotic hepatitis, and pancreatic disorders e.g. acute pancreatitis.
The invention also provides for prognostic (or predictive) assays for determining whether an individual is at risk of developing a disorder associated with NOVX protein, nucleic acid expression or activity. For example, mutations in a NOVX gene can be assayed in a biological sample. Such assays can be used for prognostic or predictive purpose to thereby prophylactically treat an individual prior to the onset of a disorder characterized by or associated with NOVX protein, nucleic acid expression, or biological activity. Another aspect of the invention provides methods for determining NOVX protein, nucleic acid expression or activity in an individual to thereby select appropriate therapeutic or prophylactic agents for that individual (referred to herein as "pharmacogenomics"). Pharmacogenomics allows for the selection of agents (e.g., drags) for therapeutic or prophylactic freatment of an individual based on the genotype of the individual (e.g., the genotype of the individual examined to determine the ability of the individual to respond to a particular agent.)
Yet another aspect of the invention pertains to monitoring the influence of agents (e.g., drags, compounds) on the expression or activity of NOVX in clinical trials. These and other agents are described in further detail in the following sections.
Diagnostic Assays
An exemplary method for detecting the presence or absence of NOVX in a biological sample involves obtaining a biological sample from a test subject and contacting the biological sample with a compound or an agent capable of detecting NOVX protein or nucleic acid (e.g., mRNA, genomic DNA) that encodes NOVX protein such that the presence of NOVX is detected in the biological sample. An agent for detecting NOVX mRNA or genomic DNA is a labeled nucleic acid probe capable of hybridizing to NOVX mRNA or genomic DNA. The nucleic acid probe can be, for example, a full-length NOVX nucleic acid, such as the nucleic acid of SEQ LD NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 or 23, or a portion thereof, such as an oligonucleotide of at least 15, 30, 50, 100, 250 or 500 nucleotides in length and sufficient to specifically hybridize under stringent conditions to NOVX mRNA or genomic DNA. Other suitable probes for use in the diagnostic assays of the invention are described herein.
One agent for detecting NOVX protein is an antibody capable of binding to NOVX protein, preferably an antibody with a detectable label. Antibodies directed against a protein of the invention may be used in methods known within the art relating to the localization and/or quantitation of the protein (e.g., for use in measuring levels of the protein within appropriate physiological samples, for use in diagnostic methods, for use in imaging the protein, and the like). In a given embodiment, antibodies against the proteins, or derivatives, fragments, analogs or homologs thereof, that contain the antigen binding domain, are utilized as pharmacologically-active compounds.
An antibody specific for a protein of the invention can be used to isolate the protein by standard techniques, such as immunoaffinity chromatography or immunoprecipitation. Such an antibody can facilitate the purification of the natural protein antigen from cells and of recombinantly produced antigen expressed in host cells. Moreover, such an antibody can be used to detect the antigenic protein (e.g., in a cellular lysate or cell supernatant) in order to evaluate the abundance and pattern of expression of the antigenic protein. Antibodies directed against the protein can be used diagnostically to monitor protein levels in tissue as part of a clinical testing procedure, e.g., to, for example, determine the efficacy of a given freatment regimen. Detection can be facilitated by coupling (i.e., physically linking) the antibody to a detectable substance. Examples of detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials, bioluminescent materials, and radioactive materials. Examples of suitable enzymes include horseradish peroxidase, alkaline phosphatase, β-galactosidase, or acetylcholinesterase; examples of suitable prosthetic group complexes include streptavidin/biotin and avidin/biotin; examples of suitable fluorescent materials include umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; an example of a luminescent material includes luminol; examples of bioluminescent materials include luciferase, luciferin, and aequorin, and examples of suitable radioactive material include !25I, 131I, 35S or 3H.
Antibodies can be polyclonal, or more preferably, monoclonal. An intact antibody, or a fragment thereof (e.g., Fab or F(ab')2) can be used. The term "labeled", with regard to the probe or antibody, is intended to encompass direct labeling of the probe or antibody by coupling (i.e., physically linking) a detectable substance to the probe or antibody, as well as indirect labeling of the probe or antibody by reactivity with another reagent that is directly labeled. Examples of indirect labeling include detection of a primary antibody using a fluorescently-labeled secondary antibody and end-labeling of a DNA probe with biotin such that it can be detected with fluorescently-labeled streptavidin. The term "biological sample" is intended to include tissues, cells and biological fluids isolated from a subject, as well as tissues, cells and fluids present within a subject. That is, the detection method of the invention can be used to detect NOVX mRNA, protein, or genomic DNA in a biological sample in vitro as well as in vivo. For example, in vitro techniques for detection of NOVX mRNA include Northern hybridizations and in situ hybridizations. In vitro techniques for detection of NOVX protein include enzyme linked immunosorbent assays (ELISAs), Western blots, immunoprecipitations, and immunofluorescence. In vitro techniques for detection of NOVX genomic DNA include Southern hybridizations. Furthermore, in vivo techniques for detection of NOVX protein include introducing into a subject a labeled anti-NOVX antibody. For example, the antibody can be labeled with a radioactive marker whose presence and location in a subject can be detected by standard imaging techniques.
In one embodiment, the biological sample contains protein molecules from the test subject. Alternatively, the biological sample can contain mRNA molecules from the test subject or genomic DNA molecules from the test subject. A preferred biological sample is a peripheral blood leukocyte sample isolated by conventional means from a subject.
In one embodiment, the methods further involve obtaining a control biological sample from a control subject, contacting the control sample with a compound or agent capable of detecting NOVX protein, mRNA, or genomic DNA, such that the presence of NOVX protein, mRNA or genomic DNA is detected in the biological sample, and comparing the presence of NOVX protein, mRNA or genomic DNA in the control sample with the presence of NOVX protein, mRNA or genomic DNA in the test sample.
The invention also encompasses kits for detecting the presence of NOVX in a biological sample. For example, the kit can comprise: a labeled compound or agent capable of detecting NOVX protein or mRNA in a biological sample; means for determining the amount of NOVX in the sample; and means for comparing the amount of NOVX in the sample with a standard. The compound or agent can be packaged in a suitable container. The kit can further comprise instructions for using the kit to detect NOVX protein or nucleic acid.
Prognostic Assays
The diagnostic methods described herein can furthermore be utilized to identify subjects having or at risk of developing a disease or disorder associated with aberrant NOVX expression or activity. For example, the assays described herein, such as the preceding diagnostic assays or the following assays, can be utilized to identify a subject having or at risk of developing a disorder associated with NOVX protein, nucleic acid expression or activity. Such disorders include for example, disorders characterized by aberrant cell proliferation, differentiation and migration, e.g. cancer, angiogenesis, atherosclerosis and obesity, neurological disorders, e.g. stroke, Pendred syndrome, multiple sclerosis and Alzheimer's disease, keratinocyte defects, e.g. lesional psoriatic skin, ischemic disorders, e.g. diabetic retinopathy, hepatic disorders, e.g. cirrhotic hepatitis, and pancreatic disorders e.g. acute pancreatitis. Alternatively, the prognostic assays can be utilized to identify a subject having or at risk for developing a disease or disorder. Thus, the invention provides a method for identifying a disease or disorder associated with aberrant NOVX expression or activity in which a test sample is obtained from a subject and NOVX protein or nucleic acid (e.g., mRNA, genomic DNA) is detected, wherein the presence of NOVX protein or nucleic acid is diagnostic for a subject having or at risk of developing a disease or disorder associated with aberrant NOVX expression or activity. As used herein, a "test sample" refers to a biological sample obtained from a subject of interest. For example, a test sample can be a biological fluid (e.g., serum), cell sample, or tissue. Furthermore, the prognostic assays described herein can be used to determine whether a subject can be administered an agent (e.g., an agonist, antagonist, peptidomimetic, protein, peptide, nucleic acid, small molecule, or other drag candidate) to treat a disease or disorder associated with aberrant NOVX expression or activity. For example, such methods can be used to determine whether a subject can be effectively treated with an agent for a disorder. Thus, the invention provides methods for determining whether a subject can be effectively treated with an agent for a disorder associated with aberrant NOVX expression or activity in which a test sample is obtained and NOVX protein or nucleic acid is detected (e.g., wherein the presence of NOVX protein or nucleic acid is diagnostic for a subject that can be administered the agent to treat a disorder associated with aberrant NOVX expression or activity).
The methods of the invention can also be used to detect genetic lesions in a NOVX gene, thereby determining if a subject with the lesioned gene is at risk for a disorder characterized by aberrant cell proliferation and/or differentiation. In various embodiments, the methods include detecting, in a sample of cells from the subject, the presence or absence of a genetic lesion characterized by at least one of an alteration affecting the integrity of a gene encoding a NOVX-protein, or the misexpression of the NOVX gene. For example, such genetic lesions can be detected by ascertaining the existence of at least one of: (i) a deletion of one or more nucleotides from a NOVX gene; (ii) an addition of one or more nucleotides to a NOVX gene; (Hi) a substitution of one or more nucleotides of a NOVX gene, (iv) a chromosomal rearrangement of a NOVX gene; (v) an alteration in the level of a messenger RNA transcript of a NOVX gene, (vi) aberrant modification of a NOVX gene, such as of the methylation pattern of the genomic DNA, (vπ) the presence of a non-wild-type splicing pattern of a messenger RNA transcript of a NOVX gene, (viii) a non- wild-type level of a NOVX protein, (ix) allelic loss of a NOVX gene, and (x) inappropriate post-translational modification of a NOVX protein. As described herein, there are a large number of assay techniques known in the art which can be used for detecting lesions in a NOVX gene. A preferred biological sample is a peripheral blood leukocyte sample isolated by conventional means from a subject. However, any biological sample containing nucleated cells may be used, including, for example, buccal mucosal cells.
In certain embodiments, detection of the lesion involves the use of a probe/primer in a polymerase chain reaction (PCR) (see, e.g., U.S. Patent Nos. 4,683,195 and 4,683,202), such as anchor PCR or RACE PCR, or, alternatively, in a ligation chain reaction (LCR) (see, e.g., Landegran, et al, 1988. Science 241: 1077-1080; and Nakazawa, et al, 1994. Proc. Natl.
Acad. Sci. USA 91: 360-364), the latter of which can be particularly useful for detecting point mutations in the NOVX-gene (see, Abravaya, et al, 1995. Nucl. Acids Res. 23: 675-682). This method can include the steps of collecting a sample of cells from a patient, isolating nucleic acid (e.g., genomic, mRNA or both) from the cells of the sample, contacting the nucleic acid sample with one or more primers that specifically hybridize to a NOVX gene under conditions such that hybridization and amplification of the NOVX gene (if present) occurs, and detecting the presence or absence of an amplification product, or detecting the size of the amplification product and comparing the length to a control sample. It is anticipated that PCR and/or LCR may be desirable to use as a preliminary amplification step in conjunction with any of the techniques used for detecting mutations described herein.
Alternative amplification methods include: self sustained sequence replication (see, Guatelli, et al, 1990. Proc. Natl. Acad. Sci. USA 87: 1874-1878), transcriptional amplification system (see, Kwoh, et al, 1989. Proc. Natl. Acad. Sci. USA 86: 1173-1177); Qβ Replicase (see, Lizardi, et al, 1988. BioTechnology 6: 1197), or any other nucleic acid amplification method, followed by the detection of the amplified molecules using techniques well known to those of skill in the art. These detection schemes are especially useful for the detection of nucleic acid molecules if such molecules are present in very low numbers.
In an alternative embodiment, mutations in a NOVX gene from a sample cell can be identified by alterations in restriction enzyme cleavage patterns. For example, sample and control DNA is isolated, amplified (optionally), digested with one or more restriction endonucleases, and fragment length sizes are determined by gel electrophoresis and compared. Differences in fragment length sizes between sample and control DNA indicates mutations in the sample DNA. Moreover, the use of sequence specific ribozymes (see, e.g., U.S. Patent No. 5,493,531) can be used to score for the presence of specific mutations by development or loss of a ribozyme cleavage site.
In other embodiments, genetic mutations in NOVX can be identified by hybridizing a sample and control nucleic acids, e.g., DNA or RNA, to high-density arrays containing hundreds or thousands of oligonucleotides probes. See, e.g., Cronin, et al, 1996. Human Mutation 7: 244-255; Kozal, et al, 1996. Nat. Med. 2: 753-759. For example, genetic mutations in NOVX can be identified in two dimensional arrays containing light-generated DNA probes as described in Cronin, et al, supra. Briefly, a first hybridization array of probes can be used to scan through long stretches of DNA in a sample and control to identify base changes between the sequences by making linear arrays of sequential overlapping probes. This step allows the identification of point mutations. This is followed by a second hybridization array that allows the characterization of specific mutations by using smaller, specialized probe arrays complementary to all variants or mutations detected. Each mutation array is composed of parallel probe sets, one complementary to the wild-type gene and the other complementary to the mutant gene.
In yet another embodiment, any of a variety of sequencing reactions known in the art can be used to directly sequence the NOVX gene and detect mutations by comparing the sequence of the sample NOVX with the corresponding wild-type (control) sequence. Examples of sequencing reactions include those based on techniques developed by Maxim and Gilbert, 1977. Proc. Natl. Acad. Sci. USA 74: 560 or Sanger, 1977. Proc. Natl. Acad. Sci. USA 74: 5463. It is also contemplated that any of a variety of automated sequencing procedures can be utilized when performing the diagnostic assays (see, e.g., Naeve, et al, 1995. Biotechniques 19: 448), including sequencing by mass specfromefry (see, e.g., PCT International Publication No. WO 94/16101; Cohen, et al, 1996. Adv. Chromatography 36: 127-162; and Griffin, et al, 1993. Appl. Biochem. Biotechnol. 38: 147-159).
Other methods for detecting mutations in the NOVX gene include methods in which protection from cleavage agents is used to detect mismatched bases in RNA/RNA or RNA/DNA heteroduplexes. See, e.g., Myers, et al, 1985. Science 230: 1242. In general, the art technique of "mismatch cleavage" starts by providing heteroduplexes of formed by hybridizing (labeled) RNA or DNA containing the wild-type NOVX sequence with potentially mutant RNA or DNA obtained from a tissue sample. The double-sfranded duplexes are freated with an agent that cleaves single-stranded regions of the duplex such as which will exist due to basepair mismatches between the control and sample strands. For instance, RNA/DNA duplexes can be freated with RNase and DNA/DNA hybrids freated with Sx nuclease to enzymatically digesting the mismatched regions, hi other embodiments, either DNA/DNA or RNA/DNA duplexes can be freated with hydroxylamine or osmium tetroxide and with piperidine in order to digest mismatched regions. After digestion of the mismatched regions, the resulting material is then separated by size on denaturing polyacrylamide gels to determine the site of mutation. See, e.g., Cotton, et al, 1988. Proc. Natl. Acad. Sci. USA 85: 4397; Saleeba, et al, 1992. Methods Enzymol. 217: 286-295. In an embodiment, the control DNA or RNA can be labeled for detection.
In still another embodiment, the mismatch cleavage reaction employs one or more proteins that recognize mismatched base pairs in double-sfranded DNA (so called "DNA mismatch repair" enzymes) in defined systems for detecting and mapping point mutations in NOVX cDNAs obtained from samples of cells. For example, the mutY enzyme of E. coli cleaves A at G/A mismatches and the thymidine DNA glycosylase from HeLa cells cleaves T at G/T mismatches. See, e.g., Hsu, et al, 1994. Carcinogenesis 15: 1657-1662. According to an exemplary embodiment, a probe based on a NOVX sequence, e.g., a wild-type NOVX sequence, is hybridized to a cDNA or other DNA product from a test cell(s). The duplex is treated with a DNA mismatch repair enzyme, and the cleavage products, if any, can be detected from electrophoresis protocols or the like. See, e.g., U.S. Patent No. 5,459,039.
In other embodiments, alterations in electrophoretic mobility will be used to identify mutations in NOVX genes. For example, single sfrand conformation polymorphism (SSCP) may be used to detect differences in electrophoretic mobility between mutant and wild type nucleic acids. See, e.g., Orita, et al, 1989. Proc. Natl. Acad. Sci. USA: 86: 2766; Cotton, 1993. Mutat. Res. 285: 125-144; Hayashi, 1992. Genet. Anal. Tech. Appl. 9: 73-79. Single-stranded DNA fragments of sample and control NOVX nucleic acids will be denatured and allowed to renature. The secondary structure of single-stranded nucleic acids varies according to sequence, the resulting alteration in electrophoretic mobility enables the detection of even a single base change. The DNA fragments may be labeled or detected with labeled probes. The sensitivity of the assay may be enhanced by using RNA (rather than DNA), in which the secondary structure is more sensitive to a change in sequence. In one embodiment, the subject method utilizes heteroduplex analysis to separate double stranded heteroduplex molecules on the basis of changes in electrophoretic mobility. See, e.g., Keen, et al, 1991. Trends Genet. 7: 5. In yet another embodiment, the movement of mutant or wild-type fragments in polyacrylamide gels containing a gradient of denaturant is assayed using denaturing gradient gel electrophoresis (DGGE). See, e.g., Myers, et al, 1985. Nature 313: 495. When DGGE is used as the method of analysis, DNA will be modified to insure that it does not completely denature, for example by adding a GC clamp of approximately 40 bp of high-melting GC-rich DNA by PCR. In a further embodiment, a temperature gradient is used in place of a denaturing gradient to identify differences in the mobility of control and sample DNA. See, e.g., Rosenbaum and Reissner, 1987. Biophys. Chem. 265: 12753.
Examples of other techniques for detecting point mutations include, but are not limited to, selective oligonucleotide hybridization, selective amplification, or selective primer extension. For example, oligonucleotide primers may be prepared in which the known mutation is placed centrally and then hybridized to target DNA under conditions that permit hybridization only if a perfect match is found. See, e.g., Saiki, et al, 1986. Nature 324: 163; Saiki, et al, 1989. Proc. Natl. Acad. Sci. USA 86: 6230. Such allele specific oligonucleotides are hybridized to PCR amplified target DNA or a number of different mutations when the oligonucleotides are attached to the hybridizing membrane and hybridized with labeled target DNA.
Alternatively, allele specific amplification technology that depends on selective PCR amplification may be used in conjunction with the instant invention. Oligonucleotides used as primers for specific amplification may carry the mutation of interest in the center of the molecule (so that amplification depends on differential hybridization; see, e.g., Gibbs, et al, 1989. Nucl. Acids Res. 17: 2437-2448) or at the extreme 3'-terminus of one primer where, under appropriate conditions, mismatch can prevent, or reduce polymerase extension (see, e.g., Prossner, 1993. Tibtech. 11 : 238). In addition it may be desirable to introduce a novel restriction site in the region of the mutation to create cleavage-based detection. See, e.g., Gasparini, et al, 1992. Mol. Cell Probes 6: . It is anticipated that in certain embodiments amplification may also be performed using Taq ligase for amplification. See, e.g., Barany, 1991. Proc. Natl. Acad. Sci. USA 88: 189. In such cases, ligation will occur only if there is a perfect match at the 3'-terminus of the 5' sequence, making it possible to detect the presence of a known mutation at a specific site by looking for the presence or absence of amplification. The methods described herein may be performed, for example, by utilizing pre-packaged diagnostic kits comprising at least one probe nucleic acid or antibody reagent described herein, which may be conveniently used, e.g., in clinical settings to diagnose patients exhibiting symptoms or family history of a disease or illness involving a NOVX gene.
Furthermore, any cell type or tissue, preferably peripheral blood leukocytes, in which NOVX is expressed may be utilized in the prognostic assays described herein. However, any biological sample containing nucleated cells may be used, including, for example, buccal mucosal cells.
Pharmacogenomics
Agents, or modulators that have a stimulatory or inhibitory effect on NOVX activity (e.g., NOVX gene expression), as identified by a screening assay described herein can be administered to individuals to treat (prophylactically or therapeutically) disorders characterized by aberrant cell proliferation, differentiation and migration, e.g. cancer, angiogenesis, atherosclerosis and obesity, neurological disorders, e.g. stroke, Pendred syndrome, multiple sclerosis and Alzheimer's disease, keratinocyte defects, e.g. lesional psoriatic skin, ischemic disorders, e.g. diabetic retinopathy, hepatic disorders, e.g. cirrhotic hepatitis, and pancreatic disorders e.g. acute pancreatitis. In conjunction with such freatment, the pharmacogenomics (i.e., the study of the relationship between an individual's genotype and that individual's response to a foreign compound or drag) of the individual may be considered. Differences in metabolism of therapeutics can lead to severe toxicity or therapeutic failure by altering the relation between dose and blood concentration of the pharmacologically active drug. Thus, the pharmacogenomics of the individual permits the selection of effective agents (e.g., drugs) for prophylactic or therapeutic treatments based on a consideration of the individual's genotype. Such pharmacogenomics can further be used to determine appropriate dosages and therapeutic regimens. Accordingly, the activity of NOVX protein, expression of NOVX nucleic acid, or mutation content of NOVX genes in an individual can be determined to thereby select appropriate agent(s) for therapeutic or prophylactic treatment of the individual.
Pharmacogenomics deals with clinically significant hereditary variations in the response to drugs due to altered drug disposition and abnormal action in affected persons. See e.g., Eichelbaum, 1996. Clin. Exp. Pharmacol Physiol, 23: 983-985; Linder, 1997. Clin. Chem., 43: 254-266. In general, two types of pharmacogenetic conditions can be differentiated. Genetic conditions transmitted as a single factor altering the way drags act on the body (altered drug action) or genetic conditions transmitted as single factors altering the way the body acts on drags (altered drug metabolism). These pharmacogenetic conditions can occur either as rare defects or as polymorphisms. For example, glucose-6-phosphate dehydrogenase (G6PD) deficiency is a common inherited enzymopathy in which the main clinical complication is hemolysis after ingestion of oxidant drugs (anti-malarials, sulfonamides, analgesics, nitrofurans) and consumption of fava beans.
As an illustrative embodiment, the activity of drag metabolizing enzymes is a major determinant of both the intensity and duration of drug action. The discovery of genetic polymorphisms of drag metabolizing enzymes (e.g., N-acetyltransferase 2 (NAT 2) and cytochrome P450 enzymes CYP2D6 and CYP2C19) has provided an explanation as to why some patients do not obtain the expected drag effects or show exaggerated drug response and serious toxicity after taking the standard and safe dose of a drug. These polymorphisms are expressed in two phenotypes in the population, the extensive metabolizer (EM) and poor metabolizer (PM). The prevalence of PM is different among different populations. For example, the gene coding for CYP2D6 is highly polymorphic and several mutations have been identified in PM, which all lead to the absence of functional CYP2D6. Poor metabohzers of CYP2D6 and CYP2C19 quite frequently experience exaggerated drug response and side effects when they receive standard doses. If a metabolite is the active therapeutic moiety, PM show no therapeutic response, as demonstrated for the analgesic effect of codeine mediated by its CYP2D6-formed metabolite morphine. At the other extreme are the so called ultra-rapid metabohzers who do not respond to standard doses. Recently, the molecular basis of ultra-rapid metabolism has been identified to be due to CYP2D6 gene amplification.
Thus, the activity of NOVX protein, expression of NOVX nucleic acid, or mutation content of NOVX genes in an individual can be determined to thereby select appropriate agent(s) for therapeutic or prophylactic freatment of the individual. In addition, pharmacogenetic studies can be used to apply genotyping of polymoφhic alleles encoding drag-metabolizing enzymes to the identification of an individual's drug responsiveness phenotype. This knowledge, when applied to dosing or drug selection, can avoid adverse reactions or therapeutic failure and thus enhance therapeutic or prophylactic efficiency when treating a subject with a NOVX modulator, such as a modulator identified by one of the exemplary screening assays described herein. Monitoring of Effects During Clinical Trials
Monitoring the influence of agents (e.g., drugs, compounds) on the expression or activity of NOVX (e.g., the ability to modulate aberrant cell proliferation) can be applied not only in basic drag screening, but also in clinical trials. For example, the effectiveness of an agent determined by a screening assay as described herein to increase NOVX gene expression, protein levels, or upregulate NOVX activity, can be monitored in clinical trails of subjects exhibiting decreased NOVX gene expression, protein levels, or downregulated NOVX activity. Alternatively, the effectiveness of an agent determined by a screening assay to decrease NOVX gene expression, protein levels, or downregulate NOVX activity, can be monitored in clinical trails of subjects exhibiting increased NOVX gene expression, protein levels, or upregulated NOVX activity. In such clinical trials, the expression or activity of NOVX and, preferably, other genes that have been implicated in, for example, a cellular proliferation or immune disorder can be used as a "read out" or markers of the immune responsiveness of a particular cell.
By way of example, and not of limitation, genes, including NOVX, that are modulated in cells by treatment with an agent (e.g., compound, drag or small molecule) that modulates NOVX activity (e.g., identified in a screening assay as described herein) can be identified. Thus, to study the effect of agents on cellular proliferation disorders, for example, in a clinical trial, cells can be isolated and RNA prepared and analyzed for the levels of expression of
NOVX and other genes implicated in the disorder. The levels of .gene expression (i.e., a gene expression pattern) can be quantified by Northern blot analysis or RT-PCR, as described herein, or alternatively by measuring the amount of protem produced, by one of the methods as described herein, or by measuring the levels of activity of NOVX or other genes. In this manner, the gene expression pattern can serve as a marker, indicative of the physiological response of the cells to the agent. Accordingly, this response state may be determined before, and at various points during, treatment of the individual with the agent.
In one embodiment, the invention provides a method for monitoring the effectiveness of treatment of a subject with an agent (e.g., an agonist, antagonist, protein, peptide, peptidomimetic, nucleic acid, small molecule, or other drag candidate identified by the screening assays described herein) comprising the steps of (i) obtaining a pre-administration sample from a subject prior to administration of the agent; (ii) detecting the level of expression of a NOVX protein, mRNA, or genomic DNA in the preadminisfration sample; (Hi) obtaining one or more post-administration samples from the subject; (iv) detecting the level of expression or activity of the NOVX protein, mRNA, or genomic DNA in the post-administration samples; (v) comparing the level of expression or activity of the NOVX protein, mRNA, or genomic DNA in the pre-administration sample with the NOVX protein, mRNA, or genomic DNA in the post administration sample or samples; and (vi) altering the admimsfration of the agent to the subject accordingly. For example, increased administration of the agent may be desirable to increase the expression or activity of NOVX to higher levels than detected, i.e., to increase the effectiveness of the agent. Alternatively, decreased administration of the agent may be desirable to decrease expression or activity of NOVX to lower levels than detected, i.e., to decrease the effectiveness of the agent.
Methods of Treatment
The invention provides for both prophylactic and therapeutic methods of treating a subject at risk of (or susceptible to) a disorder or having a disorder associated with aberrant NOVX expression or activity. Disorders associated with aberrant NOVX expression include, for example, disorders characterized by aberrant cell proliferation, differentiation and migration, e.g. cancer, angiogenesis, atherosclerosis and obesity, neurological disorders, e.g. stroke, Pendred syndrome, multiple sclerosis and Alzheimer's disease, keratinocyte defects, e.g. lesional psoriatic skin, ischemic disorders, e.g. diabetic retinopathy, hepatic disorders, e.g. cirrhotic hepatitis, and pancreatic disorders e.g. acute pancreatitis. These methods of freatment will be discussed more fully, below.
Disease and Disorders
Diseases and disorders that are characterized by increased (relative to a subject not suffering from the disease or disorder) levels or biological activity may be freated with
Therapeutics that antagonize (i.e., reduce or inhibit) activity. Therapeutics that antagonize activity may be administered in a therapeutic or prophylactic manner. Therapeutics that may be utilized include, but are not limited to: (i) an aforementioned peptide, or analogs, derivatives, fragments or homologs thereof; (ii) antibodies to an aforementioned peptide; (Hi) nucleic acids encoding an aforementioned peptide; (iv) administration of antisense nucleic acid and nucleic acids that are "dysfunctional" (i.e., due to a heterologous insertion within the coding sequences of coding sequences to an aforementioned peptide) that are utilized to "knockout" endogenous function of an aforementioned peptide by homologous recombination (see, e.g., Capecchi, 1989. Science 244: 1288-1292); or (v) modulators ( i.e., inhibitors, agonists and antagonists, including additional peptide mimetic of the invention or antibodies specific to a peptide of the invention) that alter the interaction between an aforementioned peptide and its binding partner.
Diseases and disorders that are characterized by decreased (relative to a subject not suffering from the disease or disorder) levels or biological activity may be treated with
Therapeutics that increase (i.e., are agonists to) activity. Therapeutics that upregulate activity may be administered in a therapeutic or prophylactic manner. Therapeutics that may be utilized include, but are not limited to, an aforementioned peptide, or analogs, derivatives, fragments or homologs thereof; or an agonist that increases bioavailability. Increased or decreased levels can be readily detected by quantifying peptide and/or
RNA, by obtaining a patient tissue sample (e.g., from biopsy tissue) and assaying it in vitro for RNA or peptide levels, structure and/or activity of the expressed peptides (or mRNAs of an aforementioned peptide). Methods that are well-known within the art include, but are not limited to, immunoassays (e.g., by Western blot analysis, immunoprecipitation followed by sodium dodecyl sulfate (SDS) polyacrylamide gel elecfrophoresis, immunocytochemistry, etc.) and/or hybridization assays to detect expression of mRNAs (e.g., Northern assays, dot blots, in situ hybridization, and the like).
Prophylactic Methods Lti one aspect, the invention provides a method for preventing, in a subject, a disease or condition associated with an aberrant NOVX expression or activity, by administering to the subject an agent that modulates NOVX expression or at least one NOVX activity. Subjects at risk for a disease that is caused or contributed to by aberrant NOVX expression or activity can be identified by, for example, any or a combination of diagnostic or prognostic assays as described herein. Administration of a prophylactic agent can occur prior to the manifestation of symptoms characteristic of the NOVX aberrancy, such that a disease or disorder is prevented or, alternatively, delayed in its progression. Depending upon the type of NOVX aberrancy, for example, a NOVX agonist or NOVX antagonist agent can be used for treating the subject. The appropriate agent can be determined based on screening assays described herein. The prophylactic methods of the invention are further discussed in the following subsections. Therapeutic Methods
Another aspect of the invention pertains to methods of modulating NOVX expression or activity for therapeutic purposes. The modulatory method of the invention involves contacting a cell with an agent that modulates one or more of the activities of NOVX protein activity associated with the cell. An agent that modulates NOVX protein activity can be an agent as described herein, such as a nucleic acid or a protein, a naturally-occurring cognate ligand of a NOVX protein, a peptide, a NOVX peptidomimetic, or other small molecule. In one embodiment, the agent stimulates one or more NOVX protein activity. Examples of such stimulatory agents include active NOVX protein and a nucleic acid molecule encoding NOVX that has been infroduced into the cell. In another embodiment, the agent inhibits one or more NOVX protein activity. Examples of such inhibitory agents include antisense NOVX nucleic acid molecules and anti-NOVX antibodies. These modulatory methods can be performed in vitro (e.g., by culturing the cell with the agent) or, alternatively, in vivo (e.g., by administering the agent to a subject). As such, the invention provides methods of treating an individual afflicted with a disease or disorder characterized by aberrant expression or activity of a NOVX protein or nucleic acid molecule. In one embodiment, the method involves administering an agent (e.g., an agent identified by a screening assay described herein), or combination of agents that modulates (e.g., up-regulates or down-regulates) NOVX expression or activity. In another embodiment, the method involves administering a NOVX protein or nucleic acid molecule as therapy to compensate for reduced or aberrant NOVX expression or activity.
Stimulation of NOVX activity is desirable in situations in which NOVX is abnormally downregulated and/or in which increased NOVX activity is likely to have a beneficial effect. One example of such a situation is where a subject has a disorder characterized by aberrant cell proliferation and/or differentiation (e.g., cancer or immune associated ). Another example of such a situation is where the subject has an immunodeficiency disease (e.g., ALDS).
Antibodies of the invention, including polyclonal, monoclonal, humanized and fully human antibodies, may used as therapeutic agents. Such agents will generally be employed to treat or prevent a disease or pathology in a subject. An antibody preparation, preferably one having high specificity and high affinity for its target antigen, is administered to the subject and will generally have an effect due to its binding with the target. Such an effect may be one of two kinds, depending on the specific nature of the interaction between the given antibody molecule and the target antigen in question. In the first instance, administration of the antibody may abrogate or inhibit the binding of the target with an endogenous ligand to which it naturally binds. In this case, the antibody binds to the target and masks a binding site of the naturally occurring ligand, wherein the ligand serves as an effector molecule. Thus the receptor mediates a signal fransduction pathway for which ligand is responsible.
Alternatively, the effect may be one in which the antibody elicits a physiological result by virtue of binding to an effector binding site on the target molecule. In this case the target, a receptor having an endogenous ligand which may be absent or defective in the disease or pathology, binds the antibody as a surrogate effector ligand, initiating a receptor-based signal fransduction event by the receptor.
A therapeutically effective amount of an antibody of the invention relates generally to the amount needed to achieve a therapeutic objective. As noted above, this may be a binding interaction between the antibody and its target antigen that, in certain cases, interferes with the functioning of the target, and in other cases, promotes a physiological response. The amount required to be administered will furthermore depend on the binding affinity of the antibody for its specific antigen, and will also depend on the rate at which an administered antibody is depleted from the free volume other subject to which it is administered. Common ranges for therapeutically effective dosing of an antibody or antibody fragment of the invention may be, by way of nonlimiting example, from about 0.1 mg/kg body weight to about 50 mg/kg body weight. Common dosing frequencies may range, for example, from twice daily to once a week.
Determination of the Biological Effect of the Therapeutic
In various embodiments of the invention, suitable in vitro or in vivo assays are performed to determine the effect of a specific Therapeutic and whether its administration is indicated for treatment of the affected tissue. In various specific embodiments, in vitro assays may be performed with representative cells of the type(s) involved in the patient's disorder, to determine if a given Therapeutic exerts the desired effect upon the cell type(s). Compounds for use in therapy may be tested in suitable animal model systems including, but not limited to rats, mice, chicken, cows, monkeys, rabbits, and the like, prior to testing in human subjects. Similarly, for in vivo testing, any of the animal model system known in the art may be used prior to administration to human subjects.
The invention will be further described in the following examples, which do not limit the scope of the invention described in the claims. EXAMPLES
Example 1.: Method of Identifying the Nucleic Acids of the Present Invention.
Novel nucleic acid sequences were identified by TBlastN using CuraGen Coφoration's sequence file for GPCRs or homologs run against the Genomic Daily Files made available by GenBank or from files downloaded from the individual sequencing centers. Exons were predicted by homology and the intron/exon boundaries were determined using standard genetic rules. Exons were further selected and refined by means of similarity determination using multiple BLAST (e.g., TBlastN, BlastX, and BlastN) searches, and in some instances,
GeneScan™ and Grail. Expressed sequences from both public and proprietary databases were also added when available to further define and complete gene sequences. The sequences were then manually corrected for apparent inconsistencies, thereby obtaining the sequences encoding the full-length protein.
Example 2.: Method of Cloning a NO V4 (AC020597 FG nucleic acid. The sequence of NOV4 (AC020597_FG) was derived by laboratory cloning of cDNA fragments, by in silico prediction of the sequence. cDNA fragments covering either the full length of the DNA sequence, or part of the sequence, or both, were cloned. In silico prediction was based on sequences available in CuraGen's proprietary sequence databases or in the public human sequence databases, and provided either the full length DNA sequence, or some portion thereof.
The laboratory cloning was performed using one or more of the methods summarized below:
SeqCalling™ Technology: cDNA was derived from various human samples representing multiple tissue types, normal and diseased states, physiological states, and developmental states from different donors. Samples were obtained as whole tissue, primary cells or tissue cultured primary cells or cell lines. Cells and cell lines may have been freated with biological or chemical agents that regulate gene expression, for example, growth factors, chemokines or steroids. The cDNA thus derived was then sequenced using CuraGen's proprietary SeqCalling technology. Sequence traces were evaluated manually and edited for corrections if appropriate. cDNA sequences from all samples were assembled together, sometimes including public human sequences, using bioinformatic programs to produce a consensus sequence for each assembly. Each assembly is included in CuraGen Coφoration's database. Sequences were included as components for assembly when the extent of identity with another component was at least 95%> over 50 bp. Each assembly represents a gene or portion thereof and includes information on variants, such as splice forms single nucleotide polymoφhisms (SNPs), insertions, deletions and other sequence variations.
Exon Linking: The cDNA coding for the AC020597_FG sequence was cloned by the polymerase chain reaction (PCR) using the primers:
5' AATCATGTCCATTATCAACACATCATATGTTG3' (SEQ ID NO.: 87) and
5' ACTGTTAAATCTTCCATTGACACAATTTTGC 3' (SEQ ID NO.: 88).
Primers were designed at the 5' and the 3' extremities of the predicted cDNA and were used to amplify a cDNA from a pool containing expressed human sequences derived from the following tissues: adrenal gland, bone marrow, brain - amygdala, brain - cerebellum, brain - hippocampus, brain - substantia nigra, brain - thalamus, brain -whole, fetal brain, fetal kidney, fetal liver, fetal lung, heart, kidney, lymphoma - Raji, mammary gland, pancreas, pituitary gland, placenta, prostate, salivary gland, skeletal muscle, small intestine, spinal cord, spleen, stomach, testis, thyroid, trachea and uterus. Multiple clones were sequenced and these fragments were assembled together, sometimes including public human sequences, using bioinformatic programs to produce a consensus sequence for each assembly. Each assembly is included in CuraGen Coφoration's database. Sequences were included as components for assembly when the extent of identity with another component was at least 95% over 50 bp. Each assembly represents a gene or portion thereof and includes information on variants, such as splice forms single nucleotide polymoφhisms (SNPs), insertions, deletions and other sequence variations.
Physical clone: The PCR product derived by exon linking, covering the entire open reading frame, was cloned into the pCR2.1 vector from Invitrogen to provide clone AC020597 FG.699003J5 FLC EL.
Example 3.: Method of identifying and confirming novel SNPs.
SeqCalling™ Technology: cDNA was derived from various human samples representing multiple tissue types, normal and diseased states, physiological states, and developmental states from different donors. Samples were obtained as whole tissue, cell lines, primary cells or tissue cultured primary cells and cell lines. Cells and cell lines may have been freated with biological or chemical agents that regulate gene expression for example, growth factors, chemokines, steroids. The cDNA thus derived was then sequenced using CuraGen's proprietary SeqCalling™ technology. Sequence traces were evaluated manually and edited for corrections if appropriate. cDNA sequences from all samples were assembled with themselves and with public ESTs using bioinformatics programs to generate CuraGen's human SeqCalling™ database of SeqCalling™ assemblies. Each assembly contains one or more overlapping cDNA sequences derived from one or more human samples. Fragments and ESTs were included as components for an assembly when the extent of identity with another component of the assembly was at least 95% over 50 bp. Each assembly can represent a gene and or its variants such as splice forms and/or single nucleotide polymoφhisms (SNPs) and their combinations. Variant sequences are included in this application. A variant sequence can include a
SNP. A SNP can, in some instances, be referred to as a "cSNP" to denote that the nucleotide sequence containing the SNP originates as a cDNA. A SNP can arise in several ways. For example, a SNP may be due to a substitution of one nucleotide for another at the polymoφhic site. Such a substitution can be either a transition or a transversion. A SNP can also arise from a deletion of a nucleotide or an insertion of a nucleotide, relative to a reference allele. In this case, the polymoφhic site is a site at which one allele bears a gap with respect to a particular nucleotide in another allele. SNPs occurring within genes may result in an alteration of the amino acid encoded by the gene at the position of the SNP. Intragenic SNPs may also be silent, however, in the case that a codon including a SNP encodes the same amino acid as a result of the redundancy of the genetic code. SNPs occurring outside the region of a gene, or in an infron within a gene, do not result in changes in any amino acid sequence of a protein but may result in altered regulation of the expression pattern for example, alteration in temporal expression, physiological response regulation, cell type expression regulation, intensity of expression, stability of franscribed message. Method of novel SNP Identification: SNPs are identified by analyzing sequence assemblies using CuraGen's proprietary SNPTool algorithm. SNPTool identifies variation in assemblies with the following criteria: SNPs are not analyzed within 10 base pairs on both ends of an alignment; Window size (number of bases in a view) is 10; The allowed number of mismatches in a window is 2; Minimum SNP base quality (PHRED score) is 23; Minimum number of changes to score an SNP is 2/assembly position. SNPTool analyzes the assembly and displays SNP positions, associated individual variant sequences in the assembly, the depth of the assembly at that given position, the putative assembly allele frequency, and the SNP sequence variation. Sequence traces are then selected and brought into view for manual validation. The consensus assembly sequence is imported into CuraTools along with variant sequence changes to identify potential amino acid changes resulting from the SNP sequence variation. Comprehensive SNP data analysis is then exported into the SNPCalling database. Method of novel SNP Confirmation: SNPs are confirmed employing a validated method know as Pyrosequencing. Detailed protocols for Pyrosequencing can be found in: Alderborn et al. Determination of Single Nucleotide Polymoφhisms by Real-time Pyrophosphate DNA Sequencing. (2000). Genome Research. 10, Issue 8, August. 1249- 1265.
In brief, Pyrosequencing is a real time primer extension process of genotyping. This protocol takes double-sfranded, biotinylated PCR products from genomic DNA samples and binds them to streptavidin beads. These beads are then denatured producing single stranded bound DNA. SNPs are characterized utilizing a technique based on an indirect bioluminometric assay of pyrophosphate (PP;) that is released from each dNTP upon DNA chain elongation. Following Klenow polymerase-mediated base incoφoration, PP; is released and used as a substrate, together with adenosine 5'-ρhosphosulfate (APS), for ATP sulfurylase, which results in the formation of ATP. Subsequently, the ATP accomplishes the conversion of luciferinto its oxi-derivative by the action of luciferase. The ensuing light output becomes proportional to the number of added bases, up to about four bases. To allow processivity of the method dNTP excess is degraded by apyrase, which is also present in the starting reaction mixture, so that only dNTPs are added to the template during the sequencing. The process has been fully automated and adapted to a 96-well format, which allows rapid screening of large SNP panels.
Example 4.: Quantitative expression analysis of NOVl -NOVl 2 in various cells and tissues. The quantitative expression of various clones was assessed using microtiter plates containing RNA samples from a variety of normal and pathology-derived cells, cell lines and tissues using real time quantitative PCR (RTQ PCR; TAQMAN®). RTQ PCR was performed on a Perkin-Elmer Biosystems ABI PRISM® 7700 Sequence Detection System. Various collections of samples are assembled on the plates, and referred to as Panel 1 (containing cells and cell lines from normal and cancer sources), Panel 2 (containing samples derived from tissues, in particular from surgical samples, from normal and cancer sources), Panel 3 (containing samples derived from a wide variety of cancer sources) and Panel 4 (containing cells and cell lines from normal cells and cells related to inflammatory conditions).
First, the RNA samples were normalized to constitutively expressed genes such as β- actin and GAPDH. RNA (~50 ng total or ~1 ng polyA+) was converted to cDNA using the TAQMAN® Reverse Transcription Reagents Kit (PE Biosystems, Foster City, CA; Catalog No. N808-0234) and random hexamers according to the manufacturer's protocol. Reactions were performed in 20 ul and incubated for 30 min. at 48°C. cDNA (5 ul) was then transferred to a separate plate for the TAQMAN® reaction using β-actin and GAPDH TAQMAN® Assay Reagents (PE Biosystems; Catalog Nos. 4310881E and 4310884E, respectively) and TAQMAN® universal PCR Master Mix (PE Biosystems; Catalog No. 4304447) according to the manufacturer's protocol. Reactions were performed in 25 ul using the following parameters: 2 min. at 50°C; 10 min. at 95°C; 15 sec. at 95°C/1 min. at 60°C (40 cycles). Results were recorded as CT values (cycle at which a given sample crosses a threshold level of fluorescence) using a log scale, with the difference in RNA concentration between a given sample and the sample with the lowest CT value being represented as 2 to the power of delta CT. The percent relative expression is then obtained by taking the reciprocal of this RNA difference and multiplying by 100. The average CT values obtained for β-actin and GAPDH were used to normalize RNA samples. The RNA sample generating the highest CT value required no further diluting, while all other samples were diluted relative to this sample according to their β-actin /GAPDH average CT values. -
Normalized RNA (5 ul) was converted to cDNA and analyzed via TAQMAN® using One Step RT-PCR Master Mix Reagents (PE Biosystems; Catalog No. 4309169) and gene- specific primers according to the manufacturer's instructions. Probes and primers were designed for each assay according to Perkin Elmer Biosystem's Primer Express Software package (version I for Apple Computer's Macintosh Power PC) or a similar algorithm using the target sequence as input. Default settings were used for reaction conditions and the following parameters were set before selecting primers: primer concenfration = 250 nM, primer melting temperature (T--,) range = 58°-60° C, primer optimal Tm = 59° C, maximum primer difference = 2° C, probe does not have 5' G, probe Tm must be 10° C greater than primer Tm, amplicon size 75 bp to 100 bp. The probes and primers selected (see below) were synthesized by Synthegen (Houston, TX, USA). Probes were double purified by HPLC to remove uncoupled dye and evaluated by mass specfroscopy to verify coupling of reporter and quencher dyes to the 5' and 3' ends of the probe, respectively. Their final concenfrations were: forward and reverse primers, 900 nM each, and probe, 200nM.
PCR conditions: Normalized RNA from each tissue and each cell line was spotted in each well of a 96 well PCR plate (Perkin Elmer Biosystems). PCR cocktails including two probes (a probe specific for the target clone and another gene-specific probe multiplexed with the target probe) were set up using IX TaqMan™ PCR Master Mix for the PE Biosystems 7700, with 5 mM MgC12, dNTPs (dA, G, C, U at 1:1:1:2 ratios), 0.25 U/ml AmpliTaq Gold™ (PE Biosystems), and 0.4 U/μl RNase inhibitor, and 0.25 U/μl reverse franscriptase. Reverse transcription was performed at 48° C for 30 minutes followed by amplification PCR cycles as follows: 95° C 10 min, then 40 cycles of 95° C for 15 seconds, 60° C for 1 minute.
In the results for Panel 1, the following abbreviations are used: ca. *= carcinoma,
* = established from metastasis, met = metastasis, s cell var*= small cell variant, non-s = non-sm =non-small, squam = squamous, pi. eff = pi effusion = pleural effusion, glio = glioma, astro = asfrocytoma, and neuro = neuroblastoma.
Panel 2 The plates for Panel 2 generally include 2 control wells and 94 test samples composed of RNA or cDNA isolated from human tissue procured by surgeons working in close cooperation with the National Cancer Institute's Cooperative Human Tissue Network (CHTN) or the National Disease Research Initiative (NDRI). The tissues are derived from human malignancies and in cases where indicated many malignant tissues have "matched margins" obtained from noncancerous tissue just adjacent to the tumor. These are termed normal adjacent tissues and are denoted "NAT" in the results below. The tumor tissue and the "matched margins" are evaluated by two independent pathologists (the surgical pathologists and again by a pathologists at NDRI or CHTN). This analysis provides a gross histopathological assessment of tumor differentiation grade. Moreover, most samples include the original surgical pathology report that provides information regarding the clinical stage of the patient. These matched margins are taken from the tissue surrounding (i.e. immediately proximal) to the zone of surgery (designated "NAT", for normal adjacent tissue, in Table RR). In addition, RNA and cDNA samples were obtained from various human tissues derived from autopsies performed on elderly people or sudden death victims (accidents, etc.). These tissue were ascertained to be free of disease and were purchased from various commercial sources such as Clontech (Palo Alto, CA), Research Genetics, and Invitrogen.
RNA integrity from all samples is controlled for quality by visual assessment of agarose gel electropherograms using 28S and 18S ribosomal RNA staining intensity ratio as a guide (2:1 to 2.5:1 28s:18s) and the absence of low molecular weight RNAs that would be indicative of degradation products. Samples are controlled against genomic DNA contamination by RTQ PCR reactions ran in the absence of reverse transcriptase using probe and primer sets designed to amplify across the span of a single exon.
Panel 4
Panel 4 includes samples on a 96 well plate (2 control wells, 94 test samples) composed of RNA (Panel 4r) or cDNA (Panel 4d) isolated from various human cell lines or tissues related to inflammatory conditions. Total RNA from control normal tissues such as colon and lung (Stratagene ,La Jolla, CA) and thymus and kidney (Clontech) were employed. Total RNA from liver tissue from cirrhosis patients and kidney from lupus patients was obtained from BioChain (Biochain Institute, Inc., Hayward, CA). Intestinal tissue for RNA preparation from patients diagnosed as having Crohn's disease and ulcerative colitis was obtained from the National Disease Research Interchange (NDRI) (Philadelphia, PA).
Astrocytes, lung fibroblasts, dermal fibroblasts, coronary artery smooth muscle cells, small airway epithelium, bronchial epithelium, microvascular dermal endothelial cells, microvascular lung endothelial cells, human pulmonary aortic endothelial cells, human umbilical vein endothelial cells were all purchased from Clonetics (Walkersville, MD) and grown in the media supplied for these cell types by Clonetics. These primary cell types were activated with various cytokines or combinations of cytokines for 6 and/or 12-14 hours, as indicated. The following cytokines were used; IL-1 beta at approximately 1-5 ng/ml, TNF alpha at approximately 5-10 ng/ml, IFN gamma at approximately 20-50 ng/ml, IL-4 at approximately 5-10 ng/ml, IL-9 at approximately 5-10 ng/ml, IL-13 at approximately 5-10 ng/ml. Endothelial cells were sometimes starved for various times by culture in the basal media from Clonetics with 0.1% serum.
Mononuclear cells were prepared from blood of employees at CuraGen Coφoration, using Ficoll. LAK cells were prepared from these cells by culture in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco/Life Technologies, Rockville, MD), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5 x 10"5 M (Gibco), and 10 mM Hepes (Gibco) and Interleukin 2 for 4-6 days. Cells were then either activated with 10-20 ng/ml PMA and 1-2 μg/ml ionomycin, IL-12 at 5-10 ng/ml, LFN gamma at 20-50 ng/ml and IL-18 at 5-10 ng/ml for 6 hours. In some cases, mononuclear cells were cultured for 4-5 days in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5 x 10"5 M (Gibco), and 10 mM Hepes (Gibco) with PHA (phytohemagglutinin) or PWM (pokeweed mitogen) at approximately 5 μg/ml. Samples were taken at 24, 48 and 72 hours for RNA preparation. MLR (mixed lymphocyte reaction) samples were obtained by taking blood from two donors, isolating the mononuclear cells using Ficoll and mixing the isolated mononuclear cells 1 : 1 at a final concentration of approximately 2x106 cells/ml in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol (5.5 x 10"5 M) (Gibco), and 10 mM Hepes (Gibco). The MLR was cultured and samples taken at various time points ranging from 1- 7 days for RNA preparation. Monocytes were isolated from mononuclear cells using CD 14 Miltenyi Beads, +ve VS selection columns and a Vario Magnet according to the manufacturer's instructions. Monocytes were differentiated into dendritic cells by culture in DMEM 5% fetal calf serum (FCS) (Hyclone, Logan, UT), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5 x 10"5 M (Gibco), and 10 mM Hepes (Gibco), 50 ng/ml GMCSF and 5 ng/ml IL-4 for 5-7 days. Macrophages were prepared by culture of monocytes for 5-7 days in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5 x 10"5 M (Gibco), 10 mM Hepes (Gibco) and 10% AB Human Serum or MCSF at approximately 50 ng/ml. Monocytes, macrophages and dendritic cells were stimulated for 6 and 12-14 hours with lipopolysaccharide (LPS) at 100 ng/ml. Dendritic cells were also stimulated with anti-CD40 monoclonal antibody (Pharmingen) at 10 μg/ml for 6 and 12-14 hours. CD4 lymphocytes, CD8 lymphocytes and NK cells were also isolated from mononuclear cells using CD4, CD8 and CD56 Miltenyi beads, positive VS selection columns and a Vario Magnet according to the manufacturer's instructions. CD45RA and CD45RO CD4 lymphocytes were isolated by depleting mononuclear cells of CD8, CD56, CD14 and CD19 cells using CD8, CD56, CD14 and CD19 Miltenyi beads and +ve selection. Then CD45RO beads were used to isolate the CD45RO CD4 lymphocytes with the remaining cells being CD45RA CD4 lymphocytes. CD45RA CD4, CD45RO CD4 and CD8 lymphocytes were placed in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5 x 10"5 M (Gibco), and 10 mM Hepes (Gibco) and plated at 106 cells/ml onto Falcon 6 well tissue culture plates that had been coated overnight with 0.5 μg/ml anti-CD28 (Pharmingen) and 3 ug/ml anti-CD3 (OKT3, ATCC) in PBS. After 6 and 24 hours, the cells were harvested for RNA preparation. To prepare chronically activated CD8 lymphocytes, we activated the isolated CD8 lymphocytes for 4 days on anti-CD28 and anti-CD3 coated plates and then harvested the cells and expanded them in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5 x 10"5 M (Gibco), and 10 mM Hepes (Gibco) and IL-2. The expanded CD8 cells were then activated again with plate bound anti-CD3 and anti-CD28 for 4 days and expanded as before. RNA was isolated 6 and 24 hours after the second activation and after 4 days of the second expansion culture. The isolated NK cells were cultured in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5 x 10"5 M (Gibco), and 10 mM Hepes (Gibco) and IL-2 for 4-6 days before RNA was prepared.
To obtain B cells, tonsils were procured from NDRI. The tonsil was cut up with sterile dissecting scissors and then passed through a sieve. Tonsil cells were then spun down and resupended at 106 cells/ml in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5 x 10"5 M (Gibco), and 10 mM Hepes (Gibco). To activate the cells, we used PWM at 5 μg/ml or anti-CD40 (Pharmingen) at approximately 10 μg/ml and IL-4 at 5-10 ng/ml. Cells were harvested for RNA preparation at 24,48 and 72 hours. To prepare the primary and secondary Thl/Th2 and Trl cells, six-well Falcon plates were coated overnight with 10 μg/ml anti-CD28 (Pharmingen) and 2 μg/ml OKT3 (ATCC), and then washed twice with PBS. Umbilical cord blood CD4 lymphocytes (Poietic Systems, 5 6
German Town, MD) were cultured at 10 -10 cells/ml in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5 x 10"5 M (Gibco), 10 mM Hepes (Gibco) and IL-2 (4 ng/ml). IL-12 (5 ng/ml) and anti-IL4 (1 μg/ml) were used to direct to Thl, while IL-4 (5 ng/ml) and anti-IFN gamma (1 μg/ml) were used to direct to Th2 and IL-10 at 5 ng/ml was used to direct to Trl . After 4-5 days, the activated Thl, Th2 and Trl lymphocytes were washed once in DMEM and expanded for 4-7 days in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 M sodium pyruvate (Gibco), mercaptoethanol 5.5 x 10"5 M (Gibco), 10 mM Hepes (Gibco) and IL-2 (1 ng/ml). Following this, the activated Thl, Th2 and Trl lymphocytes were re-stimulated for 5 days with anti-CD28/OKT3 and cytokines as described above, but with the addition of anti-CD95L (1 μg/ml) to prevent apoptosis. After 4-5 days, the Thl, Th2 and Trl lymphocytes were washed and then expanded again with IL-2 for 4-7 days. Activated Thl and Th2 lymphocytes were maintained in this way for a maximum of three cycles. RNA was prepared from primary and secondary Thl, Th2 and Trl after 6 and 24 hours following the second and third activations with plate bound anti-CD3 and anti-CD28 mAbs and 4 days into the second and third expansion cultures in Interleukin 2.
The following leukocyte cells lines were obtained from the ATCC: Ramos, EOL-1, KU-812. EOL cells were further differentiated by culture in 0.1 mM dbcAMP at 5 xlO5 cells/ml for 8 days, changing the media every 3 days and adjusting the cell concentration to 5 xlO5 cells/ml. For the culture of these cells, we used DMEM or RPMI (as recommended by the ATCC), with the addition of 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5 x 10"5 M (Gibco), 10 mM Hepes (Gibco). RNA was either prepared from resting cells or cells activated with PMA at 10 ng/ml and ionomycin at 1 μg/ml for 6 and 14 hours. Keratinocyte line CCD106 and an airway epithelial tumor line NCI-H292 were also obtained from the ATCC. Both were cultured in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5 x 10"5 M (Gibco), and 10 mM Hepes (Gibco). CCD1106 cells were activated for 6 and 14 hours with approximately 5 ng/ml TNF alpha and 1 ng/ml IL-1 beta, while NCI-H292 cells were activated for 6 and 14 hours with the following cytokines: 5 ng/ml IL-4, 5 ng/ml IL-9, 5 ng/ml IL-13 and 25 ng/ml LFN gamma.
For these cell lines and blood cells, RNA was prepared by lysing approximately 107 cells/ml using Trizol (Gibco BRL). Briefly, 1/10 volume of bromochloropropane (Molecular Research Coφoration) was added to the RNA sample, vortexed and after 10 minutes at room temperature, the tubes were spun at 14,000 φm in a Sorvall SS34 rotor. The aqueous phase was removed and placed in a 15 ml Falcon Tube. An equal volume of isopropanol was added and left at -20 degrees C overnight. The precipitated RNA was spun down at 9,000 rpm for 15 min in a Sorvall SS34 rotor and washed in 70% ethanol. The pellet was redissolved in 300 μl of RNAse-free water and 35 μl buffer (Promega) 5 μl DTT, 7 μl RNAsin and 8 μl DNAse were added. The tube was incubated at 37 degrees C for 30 minutes to remove contaminating genomic DNA, extracted once with phenol chloroform and re-precipitated with 1/10 volume of 3 M sodium acetate and 2 volumes of 100% ethanol. The RNA was spun down and placed in RNAse free water. RNA was stored at -80 degrees C.
A. NOVl (AC020597 E 1
Expression of gene AC020597_E was assessed using the primer-probe set Ag607, described in Table 14A. Results of the RTQ-PCR runs are shown in Tables 14B and 14C.
Table 14A. Probe Name: Ag607
Table 14B.
Table 14C. RTQ-PCR Panel 2
RTQ-PCR probe/primer sets generated against GPCRs amplify the corresponding genomic region because GPCRs are single exon genes. This is further confirmed by the expression in the genomic confrol well on each panel. The data generated in panel 2 is tainted by the genomic contamination present in panel 2 as revealed by running a probe/primer set able to amplify genomic DNA in the absence of the RT enzyme therefore in the absence of any cDNA generation. The conclusion is that the sequence has very low to undetectable expression in the panels analyzed, 1.2 and 2.
B. NOV2 (AC020597 F
Expression of gene AC020597_F was assessed using the primer-probe set Ag608, described in Table 15 A. Results of the RTQ-PCR runs for Panels 1 and 4 are shown in Table 15B. Table 15 A. Probe Name: Ag608
Table 15B. RTQ-PCR of the Primer-Probe set Ag608 using Panels 1 and 4
RTQ-PCR probe/primer sets generated against GPCRs amplify the corresponding genomic region because GPCRs are single exon genes. The expression of this gene in panel 1.1 is low to undetectable. Expression in one sample of lung cancer is unreliable due to the high Ct value. The expression of this gene in the colitis sample in panel 4 is due to genomic contamination.
C. NOV3 (A-C020597 Gl Expression of gene AC020597_G was assessed using the primer-probe set Ag609, described in Table 16 A. Results of the RTQ-PCR runs for Panels 1 and 4 are shown in Table 16B.
Table 16A. Probe Name: Ag609
Table 16B.
RTQ-PCR probe/primer sets generated against GPCRs amplify the corresponding genomic region because GPCRs are single exon genes. P. NOV7 (AC026038 B. 0050341-02^ Expression of gene AC026038_B was assessed using the primer-probe set Agl201, described in Table 17 A. Results of the RTQ-PCR runs for Panel 1 are presented in Table 17B, and those for Panels 2 and 4 are shown in Table 7C.
Expression of gene CG50341-02 was assessed using the primer-probe set Agll75, described in Table 17D. Results of the RTQ-PCR runs for Panels 1 and 4 are shown in Table 17E.
Table 17A. Probe name: Agl201
Table 17C.
RTQ-PCR probe/primer sets generated against GPCRs amplify the corresponding genomic region because GPCRs are single exon genes. This is further confirmed by the expression in the genomic control well on each panel. Table 17D. Probe Name: Agll75
Table 17E.
RTQ-PCR probe/primer sets generated against GPCRs amplify the corresponding genomic region because GPCRs are single exon genes. This is further confirmed by the expression in the genomic control well on each panel. The expression in panel 1.2 is tainted by genomic contamination, as revealed by the high expression in adipose. Expression in testis and cancer cell lines is low to undetectable (occurring at high Ct values). E. NON8 CGM 87740262 A)
Expression of gene GM_87740262_A was assessed using the primer-probe set Agll76, described in Table 18 A. Results of the RTQ-PCR runs for Panels 1 and 4 are shown in Table 18B.
Table 18A. Probe Name: Agll76
Table 18B. RTQ-PCR expression analysis of GM_87740262_A using primer-probe set Agl 176 in Panels 1 and 4.
F. NON10 (GM_ 33202597_A)
Expression of gene GM_ 33202597_A was assessed using the primer-probe set Agl 173, described in Table 19 A. Results of the RTQ-PCR runs for Panels 1 and 4 are shown in Table 19B.
Table 19A. Probe name : Agl 173
Table 19B.
RTQ-PCR probe/primer sets generated against GPCRs amplify the corresponding genomic region because GPCRs are single exon genes. This is further confirmed by the expression in the genomic control well on each panel. Expression in adipose in panel 1.2 is tainted by genomic contamination as indicated by comparison with the genomic DNA control. Tissue-specific expression of Ag1173 in panel 4D is not consistent and is therefore not amenable to analyzing utility for inflammation and immune disorders.
G. NOV12 (nh341b07_A)
Expression of gene nh341b07_A was assessed using the primer-probe set Agl 177, described in Table 20 A. Results of the RTQ-PCR runs for Panels 1 and 4 are shown in Table 20B.
Table 20A. Probe name: Agl 177
Table 20B.
RTQ-PCR probe/primer sets generated against GPCRs amplify the corresponding genomic region because GPCRs are single exon genes. This is further confirmed by the expression in the genomic control well on each panel. The level of expression of gene nh341b07_A in panel 4D is low and nonspecific.
OTHER EMBODIMENTS
While the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Other aspects, advantages, and modifications are within the scope of the following claims.

Claims

WHAT IS CLAIMED IS:
1. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
(a) a mature form of an amino acid sequence selected from the group consisting of SEQ ID NOS:2, 4, 6 , 8, 10, 12, 14, 16, 18, 20, 22 and 24;
(b) a variant of a mature form of an amino acid sequence selected from the group consisting of SEQ ID NOS:2, 4, 6 , 8, 10, 12, 14, 16, 18, 20, 22 and 24, wherein one or more amino acid residues in said variant differs from the amino acid sequence of said mature form, provided that said variant differs in no more than 15% of the amino acid residues from the amino acid sequence of said mature form;
(c) an amino acid sequence selected from the group consisting of SEQ ID NOS:2, 4, 6 , 8, 10, 12, 14, 16, 18, 20, 22 and 24; and
(d) a variant of an amino acid sequence selected from the group consisting of SEQ ID NOS.-2, 4, 6 , 8, 10, 12, 14, 16, 18, 20, 22 and 24, wherein one or more amino acid residues in said variant differs from the amino acid sequence of said mature form, provided that said variant differs in no more than 15% of amino acid residues from said amino acid sequence.
2 The polypeptide of claim 1 , wherein said polypeptide comprises the amino acid sequence of a naturally-occurring allelic variant of an amino acid sequence selected from the group consisting of SEQ ID NOS:2, 4, 6 , 8, 10, 12, 14, 16, 18, 20, 22 and 24.
3. The polypeptide of claim 2, wherein said allelic variant comprises an amino acid sequence that is the translation of a nucleic acid sequence differing by a single nucleotide from a nucleic acid sequence selected from the group consisting of SEQ ID NOS:l, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 and 23.
4. The polypeptide of claim 1, wherein the amino acid sequence of said variant comprises a conservative amino acid substitution.
5. An isolated agent that binds specifically to the polypeptide of claim 1.
6. The isolated agent of claim 5, wherein said agent is a G-protein coupled receptor ligand.
7. An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence selected from the group consisting of:
(a) a mature form of an amino acid sequence selected from the group consisting of SEQ TD NOS:2, 4, 6 , 8, 10, 12, 14, 16, 18, 20, 22 and 24;
(b) a variant of a mature form of an amino acid sequence selected from the group consisting of SEQ D NOS:2, 4, 6 , 8, 10, 12, 14, 16, 18, 20, 22 and 24, wherein one or more amino acid residues in said variant differs from the amino acid sequence of said mature form, provided that said variant differs in no more than 15% of the amino acid residues from the amino acid sequence of said mature form;
(c) an amino acid sequence selected from the group consisting of SEQ D NOS:2,
4, 6 , 8, 10, 12, 14, 16, 18, 20, 22 and 24;
(d) a variant of an amino acid sequence selected from the group consisting of SEQ ID NOS:2, 4, 6 , 8, 10, 12, 14, 16, 18, 20, 22 and 24, wherein one or more amino acid residues in said variant differs from the amino acid sequence of said mature form, provided that said variant differs in no more than 15% of amino acid residues from said amino acid sequence;
(e) a nucleic acid fragment encoding at least a portion of a polypeptide comprising an amino acid sequence chosen from the group consisting of SEQ D NOS:2, 4, 6 , 8, 10, 12, 14, 16, 18, 20, 22 and 24, or a variant of said polypeptide, wherein one or more amino acid residues in said variant differs from the amino acid sequence of said mature form, provided that said variant differs in no more than 15% of amino acid residues from said amino acid sequence; and
(f) a nucleic acid molecule comprising the complement of (a), (b), (c), (d) or (e).
8. The nucleic acid molecule of claim 7, wherein the nucleic acid molecule comprises the nucleotide sequence of a naturally-occurring allelic nucleic acid variant.
9. The nucleic acid molecule of claim 7, wherein the nucleic acid molecule encodes a polypeptide comprising the amino acid sequence of a naturally-occurring polypeptide variant.
10. The nucleic acid molecule of claim 7, wherein the nucleic acid molecule differs by a single nucleotide from a nucleic acid sequence selected from the group consisting of SEQ ID NOS:l, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 and 23.
11. The nucleic acid molecule of claim 7, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence selected from the group consisting of SEQ ID NOS: 1, 3,
5, 7, 9, 11, 13, 15, 17, 19, 21 and 23;
(b) a nucleotide sequence differing by one or more nucleotides from a nucleotide sequence selected from the group consisting of SEQ ID NOS:l, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 and 23, provided that no more than 20% of the nucleotides differ from said nucleotide sequence;
(c) a nucleic acid fragment of (a); and
(d) a nucleic acid fragment of (b).
12. The nucleic acid molecule of claim 7, wherein said nucleic acid molecule hybridizes under stringent conditions to a nucleotide sequence chosen from the group consisting of SEQ ID NOS:l, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 and 23, or a complement of said nucleotide sequence.
13. The nucleic acid molecule of claim 7, wherein the nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
(a) a first nucleotide sequence comprising a coding sequence differing by one or more nucleotide sequences from a coding sequence encoding said amino acid sequence, provided that no more than 20% of the nucleotides in the coding sequence in said first nucleotide sequence differ from said coding sequence;
(b) an isolated second polynucleotide that is a complement of the first polynucleotide; and
(c) a nucleic acid fragment of (a) or (b).
14. A vector comprising the nucleic acid molecule of claim 13.
15. The vector of claim 14, further comprising a promoter operably-linked to said nucleic acid molecule.
16. A cell comprising the vector of claim 14.
17. An antibody that binds immunospecifically to the polypeptide of claim 1.
18. The antibody of claim 17, wherein said antibody is a monoclonal antibody.
19. The antibody of claim 17, wherein the antibody is a humanized antibody.
20. A method for determining the presence or amount of the polypeptide of claim 1 in a sample, the method comprising:
(a) providing the sample;
(b) contacting the sample with an antibody that binds immunospecifically to the polypeptide; and
(c) determining the presence or amount of antibody bound to said polypeptide, thereby determining the presence or amount of polypeptide in said sample.
21. A method for determining the presence or amount of the nucleic acid molecule of claim 7 in a sample, the method comprising:
(a) providing the sample;
(b) contacting the sample with a probe that binds to said nucleic acid molecule; and
(c) determining the presence or amount of the probe bound to said nucleic acid molecule, thereby determining the presence or amount of the nucleic acid molecule in said sample.
22. The method of claim 21 wherein presence or amount of the nucleic acid molecule is used as a marker for cell or tissue type.
23. The method of claim 22 wherein the cell or tissue type is cancerous.
24. A method of identifying an agent that binds to a polypeptide of claim 1, the method comprising:
(a) contacting said polypeptide with said agent; and
(b) determining whether said agent binds to said polypeptide.
25. The method of claim 24 wherein the agent is a cellular receptor or a downstream effector.
26. The method of claim 24 wherein the agent is a G-protein coupled receptor ligand.
27. A method for identifying an agent that modulates the expression or activity of the polypeptide of claim 1, the method comprising:
(a) providing a cell expressing said polypeptide;
(b) contacting the cell with said agent, and
(c) determining whether the agent modulates expression or activity of said polypeptide, whereby an alteration in expression or activity of said peptide indicates said agent modulates expression or activity of said polypeptide.
28. A method for modulating the activity of the polypeptide of claim 1 , the method comprising contacting a cell sample expressing the polypeptide of said claim with a compound that binds to said polypeptide in an amount sufficient to modulate the activity of the polypeptide.
29. A method of treating or preventing a NOVX-associated disorder, said method comprising administering to a subject in which such treatment or prevention is desired the polypeptide of claim 1 in an amount sufficient to treat or prevent said NOVX- associated disorder in said subject.
30. The method of claim 29 wherein the disorder is selected from the group consisting of cardiomyopathy and atherosclerosis.
31. The method of claim 29 wherein the disorder is related to cell signal processing and metabolic pathway modulation.
32. The method of claim 29, wherein said subject is a human.
33. A method of treating or preventing a NOVX-associated disorder, said method comprising administering to a subject in which such treatment or prevention is desired the nucleic acid of claim 7 in an amount sufficient to treat or prevent said NOVX- associated disorder in said subject.
34. The method of claim 33 wherein the disorder is selected from the group consisting of cardiomyopathy and atherosclerosis.
35. The method of claim 33 wherein the disorder is related to cell signal processing and metabolic pathway modulation.
36. The method of claim 33, wherein said subject is a human.
37. A method of treating or preventing a NOVX-associated disorder, said method comprising administering to a subject in which such treatment or prevention is desired the antibody of claim 17 in an amount sufficient to treat or prevent said NOVX- associated disorder in said subject.
38. The method of claim 37 wherein the disorder is diabetes.
39. The method of claim 37 wherein the disorder is related to cell signal processing and metabolic pathway modulation.
40. The method of claim 37, wherein the subject is a human.
41. A pharmaceutical composition comprising the polypeptide of claim 1 and a pharmaceutically-acceptable carrier.
42. A pharmaceutical composition comprising the nucleic acid molecule of claim 7 and a pharmaceutically-acceptable carrier.
43. A pharmaceutical composition comprising the antibody of claim 17 and a pharmaceutically-acceptable carrier.
44. A kit comprising in one or more containers, the pharmaceutical composition of claim 41.
45. A kit comprising in one or more containers, the pharmaceutical composition of claim 42.
46. A kit comprising in one or more containers, the pharmaceutical composition of claim 43.
47. A method for determining the presence of or predisposition to a disease associated with altered levels of the polypeptide of claim 1 in a first mammalian subject, the method comprising:
(a) measuring the level of expression of the polypeptide in a sample from the first mammalian subject; and
(b) comparing the amount of said polypeptide in the sample of step (a) to the amount of the polypeptide present in a control sample from a second mammalian subject known not to have, or not to be predisposed to, said disease; wherein an alteration in the expression level of the polypeptide in the first subject as compared to the control sample indicates the presence of or predisposition to said disease.
48 . The method of claim 47 wherein the predisposition is to cancers.
49. A method for determining the presence of or predisposition to a disease associated with altered levels of the nucleic acid molecule of claim 7 in a first mammalian subject, the method comprising:
(a) measuring the amount of the nucleic acid in a sample from the first mammalian subject; and
(b) comparing the amount of said nucleic acid in the sample of step (a) to the amount of the nucleic acid present in a control sample from a second mammalian subject known not to have or not be predisposed to, the disease; wherein an alteration in the level of the nucleic acid in the first subject as compared to the control sample indicates the presence of or predisposition to the disease.
50. The method of claim 49 wherein the predisposition is to a cancer.
51. A method of treating a pathological state in a mammal, the method comprising administering to the mammal a polypeptide in an amount that is sufficient to alleviate the pathological state, wherein the polypeptide is a polypeptide having an amino acid sequence at least 95% identical to a polypeptide comprising an amino acid sequence of at least one of SEQ ID NOS:2, 4, 6 , 8, 10, 12, 14, 16, 18, 20, 22 and 24, or a biologically active fragment thereof.
52. A method of treating a pathological state in a mammal, the method comprising administering to the mammal the antibody of claim 17 in an amount sufficient to alleviate the pathological state.
EP01930484A 2000-04-11 2001-04-11 Gpcr-proteins and nucleic acids encoding same Withdrawn EP1272520A2 (en)

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US19599400P 2000-04-11 2000-04-11
US19653800P 2000-04-11 2000-04-11
US195994P 2000-04-11
US196538P 2000-04-11
US19990200P 2000-04-26 2000-04-26
US199902P 2000-04-26
US199964P 2000-04-26
US19994800P 2000-04-27 2000-04-27
US19995600P 2000-04-27 2000-04-27
US19996400P 2000-04-27 2000-04-27
US19995500P 2000-04-27 2000-04-27
US20017600P 2000-04-27 2000-04-27
US199955P 2000-04-27
US199956P 2000-04-27
US200176P 2000-04-27
US199948P 2000-04-27
US21899500P 2000-07-17 2000-07-17
US218995P 2000-07-17
US22064400P 2000-07-25 2000-07-25
US220644P 2000-07-25
US25964101P 2001-01-04 2001-01-04
US259641P 2001-01-04
US26485101P 2001-01-29 2001-01-29
US264851P 2001-01-29
US26856701P 2001-02-14 2001-02-14
US268567P 2001-02-14
US09/826,734 US20030017457A1 (en) 2000-04-06 2001-04-05 Novel polynucleotides and polypeptides encoded thereby
US826734P 2001-04-05
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US5874243A (en) * 1997-03-28 1999-02-23 Smithkline Beecham Corporation OLRCC15 receptor
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