EP1141382A4 - Prokaryotic system designed to monitor protease activity - Google Patents

Prokaryotic system designed to monitor protease activity

Info

Publication number
EP1141382A4
EP1141382A4 EP00908220A EP00908220A EP1141382A4 EP 1141382 A4 EP1141382 A4 EP 1141382A4 EP 00908220 A EP00908220 A EP 00908220A EP 00908220 A EP00908220 A EP 00908220A EP 1141382 A4 EP1141382 A4 EP 1141382A4
Authority
EP
European Patent Office
Prior art keywords
protease activity
system designed
prokaryotic system
monitor protease
monitor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00908220A
Other languages
German (de)
French (fr)
Other versions
EP1141382A1 (en
Inventor
Pramathesh Patel
David Lach
Michael Wittekind
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bristol Myers Squibb Co
Original Assignee
Bristol Myers Squibb Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Myers Squibb Co filed Critical Bristol Myers Squibb Co
Publication of EP1141382A1 publication Critical patent/EP1141382A1/en
Publication of EP1141382A4 publication Critical patent/EP1141382A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP00908220A 1999-01-08 2000-01-06 Prokaryotic system designed to monitor protease activity Withdrawn EP1141382A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11527099P 1999-01-08 1999-01-08
US115270P 1999-01-08
PCT/US2000/000308 WO2000040745A1 (en) 1999-01-08 2000-01-06 Prokaryotic system designed to monitor protease activity

Publications (2)

Publication Number Publication Date
EP1141382A1 EP1141382A1 (en) 2001-10-10
EP1141382A4 true EP1141382A4 (en) 2003-01-08

Family

ID=22360285

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00908220A Withdrawn EP1141382A4 (en) 1999-01-08 2000-01-06 Prokaryotic system designed to monitor protease activity

Country Status (5)

Country Link
EP (1) EP1141382A4 (en)
JP (1) JP2002534095A (en)
AU (1) AU764132B2 (en)
CA (1) CA2360022A1 (en)
WO (1) WO2000040745A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2364384A1 (en) * 1999-03-05 2000-09-08 Glaxo Group Limited Assays for inhibitors of ftsh
AU2001281734A1 (en) * 2000-08-29 2002-03-13 Novozymes A/S Method for screening highly active proteases and inhibitors
KR20050096954A (en) * 2003-02-04 2005-10-06 가부시키가이샤 야쿠루트 혼샤 Breast cancer resistance protein(bcrp) inhibitor
AU2005336856A1 (en) * 2005-09-27 2007-04-05 Oncalis Ag Genetic selection system to identify proteases, protease substrates and protease inhibitors
EP3600365A4 (en) 2017-03-29 2021-01-06 President and Fellows of Harvard College Methods of regulating gene expression in a cell

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5599906A (en) * 1990-04-13 1997-02-04 Schering Corporation Protease assays

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DASMAHAPATRA B ET AL: "A GENETIC SYSTEM FOR STUDYING THE ACTIVITY OF A PROTEOLYTIC ENZYME", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 89, May 1992 (1992-05-01), pages 4159 - 4162, XP000946643, ISSN: 0027-8424 *
FIELDS S ET AL: "THE TWO-HYBRID SYSTEM: AN ASSAY FOR PROTEIN-PROTEIN INTERACTIONS", TRENDS IN GENETICS, ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, NL, vol. 10, no. 8, 1 August 1994 (1994-08-01), pages 286 - 292, XP000647708, ISSN: 0168-9525 *
LAWLER JOSEPH F JR ET AL: "Viral protease assay based on GAL4 inactivation is applicable to high-throughput screening in mammalian cells.", ANALYTICAL BIOCHEMISTRY, vol. 269, no. 1, 10 April 1999 (1999-04-10), pages 133 - 138, XP002220088, ISSN: 0003-2697 *
LIEBIG H D ET AL: "PROTEINASE TRAPPING: SCREENING FOR VIRAL PROTEINASE MUTANTS BY ALPHA COMPLEMENTATION", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 88, no. 14, 15 July 1991 (1991-07-15), pages 5979 - 5983, XP000248161, ISSN: 0027-8424 *
See also references of WO0040745A1 *

Also Published As

Publication number Publication date
WO2000040745A1 (en) 2000-07-13
EP1141382A1 (en) 2001-10-10
JP2002534095A (en) 2002-10-15
AU2960700A (en) 2000-07-24
CA2360022A1 (en) 2000-07-13
AU764132B2 (en) 2003-08-14

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Legal Events

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Inventor name: WITTEKIND, MICHAEL

Inventor name: LACH, DAVID

Inventor name: PATEL, PRAMATHESH

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