EP1137940A4 - Assays for ligands for nuclear receptors - Google Patents

Assays for ligands for nuclear receptors

Info

Publication number
EP1137940A4
EP1137940A4 EP99971090A EP99971090A EP1137940A4 EP 1137940 A4 EP1137940 A4 EP 1137940A4 EP 99971090 A EP99971090 A EP 99971090A EP 99971090 A EP99971090 A EP 99971090A EP 1137940 A4 EP1137940 A4 EP 1137940A4
Authority
EP
European Patent Office
Prior art keywords
ligands
assays
nuclear receptors
receptors
nuclear
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP99971090A
Other languages
German (de)
French (fr)
Other versions
EP1137940A1 (en
Inventor
Steven Gerard Blanchard
Derek J Parks
Julie Beth Stimmel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Glaxo Group Ltd
Original Assignee
Glaxo Group Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glaxo Group Ltd filed Critical Glaxo Group Ltd
Publication of EP1137940A1 publication Critical patent/EP1137940A1/en
Publication of EP1137940A4 publication Critical patent/EP1137940A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
EP99971090A 1998-10-23 1999-10-22 Assays for ligands for nuclear receptors Withdrawn EP1137940A4 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US10539098P 1998-10-23 1998-10-23
US105390P 1998-10-23
US13509798P 1998-12-23 1998-12-23
US135097P 1998-12-23
US13483699P 1999-05-19 1999-05-19
US134836P 1999-05-19
PCT/US1999/024956 WO2000025134A1 (en) 1998-10-23 1999-10-22 Assays for ligands for nuclear receptors

Publications (2)

Publication Number Publication Date
EP1137940A1 EP1137940A1 (en) 2001-10-04
EP1137940A4 true EP1137940A4 (en) 2004-06-02

Family

ID=27379915

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99971090A Withdrawn EP1137940A4 (en) 1998-10-23 1999-10-22 Assays for ligands for nuclear receptors

Country Status (4)

Country Link
EP (1) EP1137940A4 (en)
JP (1) JP2002528721A (en)
AU (1) AU1229000A (en)
WO (1) WO2000025134A1 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000037077A1 (en) 1998-12-23 2000-06-29 Glaxo Group Limited Assays for ligands for nuclear receptors
WO2002077229A2 (en) * 2001-03-01 2002-10-03 Lion Bioscience Ag Cofactors of the liver x receptor alpha and methods of use
US7138390B2 (en) 2001-03-12 2006-11-21 Intercept Pharmaceuticals Steroids as agonists for FXR
WO2003059884A1 (en) 2001-12-21 2003-07-24 X-Ceptor Therapeutics, Inc. Modulators of lxr
US7482366B2 (en) 2001-12-21 2009-01-27 X-Ceptor Therapeutics, Inc. Modulators of LXR
US6696473B2 (en) 2001-12-21 2004-02-24 X-Ceptor Therapeutics, Inc. Heterocyclic modulators of nuclear receptors
US6987121B2 (en) 2002-04-25 2006-01-17 Smithkline Beecham Corporation Compositions and methods for hepatoprotection and treatment of cholestasis
JP4803976B2 (en) * 2003-07-09 2011-10-26 独立行政法人科学技術振興機構 Molecular sensor for intracellular IP3 measurement
DK2712617T3 (en) 2004-03-12 2017-02-13 Intercept Pharmaceuticals Inc Treatment of fibrosis with Fxr ligands.
US10987362B2 (en) 2004-03-12 2021-04-27 Intercept Pharmaceuticals, Inc. Treatment of fibrosis using FXR ligands
ITMI20050912A1 (en) 2005-05-19 2006-11-20 Erregierre Spa PROCESS OF PREPARATION OF ACIDS 3-A-YA (B) -DIDROSSI-6-A (B) -ALCHIL-5B-COLANICI
DK2080012T3 (en) * 2006-11-10 2013-06-17 Dimerix Bioscience Pty Ltd Methods for analyzing test compounds at associated receptors.
AU2008209566C1 (en) 2007-01-19 2013-02-14 Intercept Pharmaceuticals, Inc. 23-substituted bile acids as TGR5 modulators and methods of use thereof
EP2323685A2 (en) * 2008-07-11 2011-05-25 Medizinische Universität Innsbruck Agonists of nr2f6 for immunosuppression
AU2009276507B2 (en) 2008-07-30 2015-11-19 Intercept Pharmaceuticals, Inc. TGR5 modulators and methods of use thereof
EA020140B1 (en) 2008-11-19 2014-08-29 Интерсепт Фармасьютикалз, Инк. Tgr5 modulators and method of use thereof
EP4215194A1 (en) 2011-01-11 2023-07-26 Dimerix Bioscience Pty Ltd Combination therapy
DK3336097T3 (en) 2012-06-19 2020-09-28 Intercept Pharmaceuticals Inc Preparation of non-crystalline obeticholic acid
US9982008B2 (en) 2012-06-19 2018-05-29 Intercept Pharmaceuticals, Inc. Preparation and uses of obeticholic acid
US9074186B2 (en) 2012-08-15 2015-07-07 Boston Medical Center Corporation Production of red blood cells and platelets from stem cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996021742A1 (en) * 1995-01-13 1996-07-18 The Salk Institute For Biological Studies Method for modulating processes mediated by farnesoid activated receptors
WO1998032444A1 (en) * 1997-01-24 1998-07-30 The Regents Of The University Of California USE OF FXR, PPARα AND LXRα ACTIVATORS TO RESTORE BARRIER FUNCTION, PROMOTE EPIDERMAL DIFFERENTIATION AND INHIBIT PROLIFERATION
WO1999018124A1 (en) * 1997-10-07 1999-04-15 Merck & Co., Inc. Assays for nuclear receptor ligands using fret
WO1999050664A1 (en) * 1998-04-01 1999-10-07 Glaxo Group Limited Compositions and methods for detecting ligand-dependent nuclear receptor and coactivator interactions

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4568649A (en) * 1983-02-22 1986-02-04 Immunex Corporation Immediate ligand detection assay
DK39892D0 (en) * 1992-03-25 1992-03-25 Bernard Thorens PEPTIDE
US5776699A (en) * 1995-09-01 1998-07-07 Allergan, Inc. Method of identifying negative hormone and/or antagonist activities

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996021742A1 (en) * 1995-01-13 1996-07-18 The Salk Institute For Biological Studies Method for modulating processes mediated by farnesoid activated receptors
WO1998032444A1 (en) * 1997-01-24 1998-07-30 The Regents Of The University Of California USE OF FXR, PPARα AND LXRα ACTIVATORS TO RESTORE BARRIER FUNCTION, PROMOTE EPIDERMAL DIFFERENTIATION AND INHIBIT PROLIFERATION
WO1999018124A1 (en) * 1997-10-07 1999-04-15 Merck & Co., Inc. Assays for nuclear receptor ligands using fret
WO1999050664A1 (en) * 1998-04-01 1999-10-07 Glaxo Group Limited Compositions and methods for detecting ligand-dependent nuclear receptor and coactivator interactions

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
DONG DIANE ET AL: "Heterodimer formation by retinoid X receptor: Regulation by ligands and by the receptor's self-association properties", BIOCHEMISTRY, vol. 37, no. 30, 28 July 1998 (1998-07-28), &, pages 10691 - 10700, XP001180506, ISSN: 0006-2960 *
HAUSSLER M R ET AL: "THE VITAMIN D HORMONE AND ITS NUCLEAR RECEPTOR: MOLECULAR ACTIONS AND DISEASE STATES", JOURNAL OF ENDOCRINOLOGY, BRISTOL, GB, vol. 154, September 1997 (1997-09-01), pages S57 - S73, XP000867746, ISSN: 0022-0795 *
KREY G ET AL: "FATTY ACIDS, EICOSANOIDS, AND HYPOLIPIDEMIC AGENTS IDENTIFIED AS LIGANDS OF PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORS BY COACTIVATOR-DEPENDENT RECEPTOR LIGAND ASSAY", MOLECULAR ENDOCRINOLOGY, BALTIMORE, MD, US, vol. 11, no. 6, June 1997 (1997-06-01), pages 779 - 791, XP002915086, ISSN: 0888-8809 *
MINUCCI SAVERIO ET AL: "Retinoid X receptor (RXR) within the RXR-retinoic acid receptor heterodimer binds its ligand and enhances retinoid-dependent gene expression", MOLECULAR AND CELLULAR BIOLOGY, vol. 17, no. 2, 1997, &, pages 644 - 655, XP002274485, ISSN: 0270-7306 *
NICHOLS JAMES S ET AL: "Development of a scintillation proximity assay for peroxisome proliferator-activated receptor gamma ligand binding domain.", ANALYTICAL BIOCHEMISTRY, vol. 257, no. 2, 15 March 1998 (1998-03-15), pages 112 - 119, XP002214931, ISSN: 0003-2697 *
NIESOR ERIC J ET AL: "Synthetic farnesoid X receptor (FXR) agonists: A new class of cholesterol synthesis inhibitors and antiproliferative drugs", DRUGS OF THE FUTURE, vol. 24, no. 4, April 1999 (1999-04-01), &, pages 431 - 438, XP009028203, ISSN: 0377-8282 *
NOLTE R T ET AL: "LIGAND BINDING AND CO-ACTIVATOR ASSEMBLY OF THE PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR-GAMMA", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 395, 10 September 1998 (1998-09-10), pages 137 - 143, XP002906503, ISSN: 0028-0836 *
PARKS DEREK J ET AL: "Bile acids: Natural ligands for an orphan nuclear receptor.", SCIENCE (WASHINGTON D C), vol. 284, no. 5418, 21 May 1999 (1999-05-21), pages 1365 - 1368, XP000892009, ISSN: 0036-8075 *
See also references of WO0025134A1 *
ZHOU G ET AL: "NUCLEAR RECEPTORS HAVE DISTINCT AFFINITIES FOR COACTIVATORS: CHARACTERIZATION BY FLUORESCENCE RESONANCE ENERGY TRANSFER", MOLECULAR ENDOCRINOLOGY, BALTIMORE, MD, US, vol. 12, no. 10, October 1998 (1998-10-01), pages 1594 - 1604, XP002934409, ISSN: 0888-8809 *

Also Published As

Publication number Publication date
JP2002528721A (en) 2002-09-03
WO2000025134A1 (en) 2000-05-04
EP1137940A1 (en) 2001-10-04
AU1229000A (en) 2000-05-15

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