EP1124425A4 - Insecticidal composition - Google Patents

Insecticidal composition

Info

Publication number
EP1124425A4
EP1124425A4 EP00954174A EP00954174A EP1124425A4 EP 1124425 A4 EP1124425 A4 EP 1124425A4 EP 00954174 A EP00954174 A EP 00954174A EP 00954174 A EP00954174 A EP 00954174A EP 1124425 A4 EP1124425 A4 EP 1124425A4
Authority
EP
European Patent Office
Prior art keywords
oil
composition
extract
stipitata
insecticidally effective
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00954174A
Other languages
German (de)
French (fr)
Other versions
EP1124425A1 (en
Inventor
Brian Milgate
Albert Habib Basta
Robert Neil Spooner-Hart
David William Tayler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Australian Native Foods Resource Development Pty Ltd
Original Assignee
Australian Native Foods Resource Development Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Australian Native Foods Resource Development Pty Ltd filed Critical Australian Native Foods Resource Development Pty Ltd
Publication of EP1124425A1 publication Critical patent/EP1124425A1/en
Publication of EP1124425A4 publication Critical patent/EP1124425A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof

Definitions

  • the present invention relates to an insecticidal composition including Tasmannia stipitata extract and insecticidally effective oils such as petroleum oils, vegetable oils and/or fish oils.
  • the invention also relates to a method of controlling arthropods pests using combinations of Tasmannia stipitata extract and insecticidally effective oils such as petroleum oils, vegetable oils and/or fish oils.
  • Background Art The genus Tasmannia was formerly known as Drimys.
  • Tasmannia stipitata also known previously as Tasmannia aromatica var. pendunculata or Drimys aromatica
  • Dorrigo Pepper is commonly known as Dorrigo Pepper. It is rich in the compound polygodial.
  • Polygodial is used in foods for its hot and spicy taste. It also has been found to have antifungal, antibacterial and insecticidal activity.
  • Extracts of leave, stems and berries include polygodial as well as linalool, pinene , copaene, caryophyllene and bicyclo-germacrene.
  • Petroleum oils or mineral oils are used as insecticides. Certain vegetable oils also have insecticidal activity. The most common is canola oil (rapeseed oil). Sesame oil is also used. Other vegetable oils having insecticidal activity include mustard oil. A third group of oils having insecticidal activity is fish oil. Disclosure of Invention
  • the present invention provides an insecticidal or insect repellant composition including Tasmannia stipitata extract and an insecticidally effective oil selected from the group consisting of at least one petroleum oil. at least one vegetable oil, at least one fish oil and mixtures thereof.
  • insecticidal activity the composition acts as an insect repellent. It has also been found that insects that come into contact with a surface that has been treated with the composition exhibit reduced feeding and oviposition (egg laying) behaviour. The composition may therefore be used to control insects.
  • the present invention provides a method of controlling or killing insects comprising exposing an insect population to a composition including Tasmannia stipitata extract and an insecticidally effective oil selected from the group consisting of at least one petroleum oil, at least one vegetable oil, at least one fish oil and mixtures thereof.
  • the present invention provides for the use of Tasmannia stipitata extract to enhance the insecticidal activity of an oil selected from the group consisting of at least one petroleum oil. at least one vegetable oil. at least one fish oil and mixtures thereof.
  • the extract of Tasmannia stipitata is preferably prepared by solvent extraction of new leaves, new stems and berries.
  • the concentration of polygodial in the extract will vary with the relative proportions of leaves, stems and berries extracted and will also vary with season. Concentrations of about 10% or more polygodial are desirable in the extract, preferably about 10-40% and more preferably about 25-38%.
  • the concentration of Tasmannia stipitata extract in the insecticidal composition may range from about 0.01% w/v to about 1.25% w/v. preferably 0.02% w/v to 1.0% w/v, more preferably 0.03% w/v to 0.125%w/v.
  • the desired concentration will vary with the polygodial content of the Tasmannia stipitata extract.
  • the concentration of insecticidally effective oil in the insecticidal composition may range from about 0.25% w/v to about 1.5% w/v, preferably 0.5% to 1.0% w/v.
  • composition of the invention may be applied to insect populations in a variety of ways, spraying is one preferred means.
  • Application of a spray may be achieved using aerosols, suitably pressurised dispensers or other pumping devices.
  • Modes for Carrying Out the Invention Materials 1. Samples of Tasmannia stipitata extract (hereinafter referred to as "stipitata extract”) were prepared by solvent extraction of mixtures of new leaves, new stems and berries of Tasmannia stipitata. These samples contained an average of 27.6% polygodial.
  • the polygodial content of the samples was ascertained as follows: A known weight of stipitata extract was dissolved in 5 mL of ethanol with an internal standard of tridecane at a concentration of 2mg/mL. This solution was analysed by gas chromatography and the polygodial was assessed by area ratio to the tridecane and assumption that the response factor between the two compounds equals 1. The average percent of polygodial stated above is the average percent of volatiles in the extract.
  • D-C-Tron Plus® spray oil, supplied by Ampol, active ingredient 839g/L petroleum oil (referred to in the tables as "Petroleum Oil 2").
  • the leaf discs were placed on moist cotton wool covered with muslin netting. Water was added to the dish daily to prevent the desiccation of the leaf discs.
  • the dose of product for testing was applied by means of a Potter Precision Spray Tower and a 5ml aliquot was used for each Petrie dish.
  • the mortality (number killed) was assessed 24. 48 and 72 hours after treatment. Death was recognised by the absence of movement when the test mites were mechanically stimulated.
  • the LD 0S of Stipitata extract alone is improved from 0.12% (a) to 0.08% (e) when blended with 1% canola oil (f).
  • the LD Q5 of canola oil is improved from 3.71% (g) to 1% (f) with the addition of Stipitata extract.
  • the LD 50 of Stipitata extract alone is improved from 0.03% (t) to 0.006% (u) when blended with 0.5% petroleum oil (v).
  • the LD 50 of petroleum oil is simultaneously improved from 1.23% (w) to 0.5% (v) with the addition of Stipitata extract.
  • the LD 50 of Stipitata extract is improved from 0.03% (t) to 0.017% (y) when blended with 1% canola oil (z).
  • the LD 50 of canola oil is simultaneously improved from 1.69% (x) to 1% (z) with the addition of Stipitata extract
  • Synergistic activity as an insecticide against a broad range of insects is expected including thrips, aphids. scales, mites, caterpillars, lice and flies.
  • Industrial Applicability Combining Tasmannia stipitata extract with insecticidally effective oils including petroleum oil. vegetable oil, and fish oil gives a highly effective insecticidal composition.
  • the proportion of active ingredients in the composition required for effective control of the insect pest can be substantially reduced compared to the proportion required for each ingredient when used alone.
  • Phytotoxicity of the composition is significantly reduced by reduced actives levels, as is the cost of manufacturing the composition. It is expected that the insecticidal composition will be effective against a broad range of insects.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

An insecticidal composition is disclosed that includes Tasmannia stipitata extract in combination with an insecticidally effective oil such as petroleum oil, vegetable oil, and fish oil. The Tasmannia stipitata extract enhances the insecticidal activity of these oils. It is particularly useful against a variety of insect pests that occur on plants such as thrips, aphids, mites, scales and caterpillars. It is also useful against lice and flies.

Description

INSECTICIDAL COMPOSITION Technical Field
The present invention relates to an insecticidal composition including Tasmannia stipitata extract and insecticidally effective oils such as petroleum oils, vegetable oils and/or fish oils. The invention also relates to a method of controlling arthropods pests using combinations of Tasmannia stipitata extract and insecticidally effective oils such as petroleum oils, vegetable oils and/or fish oils. Background Art The genus Tasmannia was formerly known as Drimys. Tasmannia stipitata (also known previously as Tasmannia aromatica var. pendunculata or Drimys aromatica) is commonly known as Dorrigo Pepper. It is rich in the compound polygodial.
Polygodial is used in foods for its hot and spicy taste. It also has been found to have antifungal, antibacterial and insecticidal activity.
The active ingredients of Tasmannia stipitata appear to be contained within oil cells concentrated in new leaves, new stems and berries. Extracts of leave, stems and berries include polygodial as well as linalool, pinene , copaene, caryophyllene and bicyclo-germacrene. Petroleum oils (or mineral oils) are used as insecticides. Certain vegetable oils also have insecticidal activity. The most common is canola oil (rapeseed oil). Sesame oil is also used. Other vegetable oils having insecticidal activity include mustard oil. A third group of oils having insecticidal activity is fish oil. Disclosure of Invention
Combinations of Tasmannia stipitata extract and petroleum oil or insecticidally effective vegetable oil have been found to have unexpected synergistic activity as insecticides. It is assumed that the insecticidal activity of fish oils would be similarly enhanced in combination with Tasmannia stipitata extract.
Accordingly, the present invention provides an insecticidal or insect repellant composition including Tasmannia stipitata extract and an insecticidally effective oil selected from the group consisting of at least one petroleum oil. at least one vegetable oil, at least one fish oil and mixtures thereof. In addition to insecticidal activity, the composition acts as an insect repellent. It has also been found that insects that come into contact with a surface that has been treated with the composition exhibit reduced feeding and oviposition (egg laying) behaviour. The composition may therefore be used to control insects.
In another aspect, the present invention provides a method of controlling or killing insects comprising exposing an insect population to a composition including Tasmannia stipitata extract and an insecticidally effective oil selected from the group consisting of at least one petroleum oil, at least one vegetable oil, at least one fish oil and mixtures thereof.
In a further aspect, the present invention provides for the use of Tasmannia stipitata extract to enhance the insecticidal activity of an oil selected from the group consisting of at least one petroleum oil. at least one vegetable oil. at least one fish oil and mixtures thereof. The extract of Tasmannia stipitata is preferably prepared by solvent extraction of new leaves, new stems and berries. The concentration of polygodial in the extract will vary with the relative proportions of leaves, stems and berries extracted and will also vary with season. Concentrations of about 10% or more polygodial are desirable in the extract, preferably about 10-40% and more preferably about 25-38%.
The concentration of Tasmannia stipitata extract in the insecticidal composition may range from about 0.01% w/v to about 1.25% w/v. preferably 0.02% w/v to 1.0% w/v, more preferably 0.03% w/v to 0.125%w/v. The desired concentration will vary with the polygodial content of the Tasmannia stipitata extract.
The concentration of insecticidally effective oil in the insecticidal composition may range from about 0.25% w/v to about 1.5% w/v, preferably 0.5% to 1.0% w/v.
Although it will be appreciated that the composition of the invention may be applied to insect populations in a variety of ways, spraying is one preferred means. Application of a spray may be achieved using aerosols, suitably pressurised dispensers or other pumping devices.
Insects of course reside in a variety of environments. For those environments that are animate, such as in the case of plants and animals that are infested with insects, the present invention is advantageous in that it has reduced toxicity. This arises out of the fact that due to enhanced insecticidal activity, less active material need be applied to the plant or animal. Modes for Carrying Out the Invention Materials 1. Samples of Tasmannia stipitata extract (hereinafter referred to as "stipitata extract") were prepared by solvent extraction of mixtures of new leaves, new stems and berries of Tasmannia stipitata. These samples contained an average of 27.6% polygodial. The polygodial content of the samples was ascertained as follows: A known weight of stipitata extract was dissolved in 5 mL of ethanol with an internal standard of tridecane at a concentration of 2mg/mL. This solution was analysed by gas chromatography and the polygodial was assessed by area ratio to the tridecane and assumption that the response factor between the two compounds equals 1. The average percent of polygodial stated above is the average percent of volatiles in the extract.
2. D-C-Tron Plus®, spray oil, supplied by Ampol, active ingredient 839g/L petroleum oil (referred to in the tables as "Petroleum Oil 2").
3. Fasta® oil, supplied by Cobbett Pty Ltd. 704 g/L vegetable oil. primarily canola oil (hereinafter referred to as "canola oil"). 4. White oil spray, supplied by Yates, active ingredient 820g/L petroleum oil (hereinafter referred to as "white oil" and in the tables as "Petroleum Oil 1"). Process
Stipitata extract in amounts shown in column A of the table below was combined with oils B. C or D respectively in amounts as shown below.
Each of these blends was diluted with distilled water in a lOOnil volumetric flask to prepare the following mixtures: Stipitata extract(ml) and oil(ml) (1.25 and 10), (0.625 and 10), (0.3125 and 10) and (1.25 and 5) per 100ml dilution.
From these homogenised stock solutions, further serial dilutions to (0.125 and 1), (0.0625 and 1), (0.03125 and 1). (0.125 and 0.5), (0.0625 and 0.5) and (0.03125 and 0.5) per cent were prepared by mixing the required amount of the stock solution in distilled water. For Stipitata extract alone, one gram was dissolved in 20ml of absolute ethanol and distilled water was added to prepare a 1% stock solution. A drop weighing 40mg Triton X-100® surfactant supplied by Union Carbide Chemicals and Plastic Co, was added to the stock solution. Further serial dilutions of 0.01. 0.02, 0.04, 0.06 and 0.08% were prepared by mixing the required amount of stock solution with distilled water.
For each oil alone a 10% w/v emulsion in distilled water and further dilutions of 2.0. 1.8, 1.6, 1.4, 1.2 1,0, 0.50 and 0.25% were prepared by adding to the required amount of distilled water.
Adult female two-spotted mite [Tetranychus urticae) were collected. Tests were conducted on 80 females for each treatment, distributed on four French bean leaf discs 25mm diameter, contained in 90mm diameter Petrie dishes. Each treatment was repeated twice.
The leaf discs were placed on moist cotton wool covered with muslin netting. Water was added to the dish daily to prevent the desiccation of the leaf discs.
The dose of product for testing was applied by means of a Potter Precision Spray Tower and a 5ml aliquot was used for each Petrie dish.
The mortality (number killed) was assessed 24. 48 and 72 hours after treatment. Death was recognised by the absence of movement when the test mites were mechanically stimulated.
Probit analysis based on the concentration of Stipitata extract in the mixtures was carried out to interpret the dose mortality relationship. Results
Results are summarised in the tables below.
Combined with all the oils outlined above. Stipitata extract was more efficacious (ie. caused higher mortality) than when used alone. In addition, for oils to which Stipitata extract had been added, efficacy was significantly enhanced. For oils used alone, mortality was lowest of all treatments. The results above demonstrate synergistic insecticidal effect against
Two-spotted mite by Tasmannia stipitata extract mixed with different types of oils. The LD05 of Stipitata extract alone is improved from 0.12% (a) to 0.048% (b) when blended with 0.5% petroleum oil. Simultaneously the LDg5 of petroleum oil is improved from 2.72% (c) to 0.5% (d) with the addition of Stipitata extract.
The LD0S of Stipitata extract alone is improved from 0.12% (a) to 0.08% (e) when blended with 1% canola oil (f). Simultaneously the LDQ5 of canola oil is improved from 3.71% (g) to 1% (f) with the addition of Stipitata extract. The LD50 of Stipitata extract alone is improved from 0.03% (t) to 0.006% (u) when blended with 0.5% petroleum oil (v). The LD50 of petroleum oil is simultaneously improved from 1.23% (w) to 0.5% (v) with the addition of Stipitata extract. The LD50 of Stipitata extract is improved from 0.03% (t) to 0.017% (y) when blended with 1% canola oil (z). The LD50 of canola oil is simultaneously improved from 1.69% (x) to 1% (z) with the addition of Stipitata extract
Synergistic activity as an insecticide against a broad range of insects is expected including thrips, aphids. scales, mites, caterpillars, lice and flies. Industrial Applicability Combining Tasmannia stipitata extract with insecticidally effective oils including petroleum oil. vegetable oil, and fish oil gives a highly effective insecticidal composition. The proportion of active ingredients in the composition required for effective control of the insect pest can be substantially reduced compared to the proportion required for each ingredient when used alone. Phytotoxicity of the composition is significantly reduced by reduced actives levels, as is the cost of manufacturing the composition. It is expected that the insecticidal composition will be effective against a broad range of insects. It will be appreciated by persons skilled in the art that numerous variations and/or modifications may be made to the invention as shown in the specific embodiments without departing from the spirit or scope of the invention as broadly described. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive.

Claims

CLAIMS:
1. An insecticidal or insect repellant composition including Tasmannia stipitata extract and an insecticidally effective oil selected from the group consisting of at least one petroleum oil, at least one vegetable oil. at least one fish oil and mixtures thereof.
2. An insecticidal or insect repellant composition as claimed in claim 1 wherein the Tasmannia stipitata extract is included in the composition in an amount of from about 0.01% to about 1.25% w/v, preferably 0.02% to 1.0% w/v, most preferably 0.03% to 0.125% w/v.
3. An insecticidal or insect repellant composition as claimed in claim 1 or claim 2 wherein the insecticidally effective oil is included in the composition in an amount of from about 0.25% to about 1.5% w/v, preferably 0.5% to 1.0% w/v.
4. An insecticidal or insect repellant composition as claimed in any one of claim 1 to 3 wherein the insecticidally effective oil is petroleum oil.
5. An insecticidal or insect repellant composition as claimed in any one of claims 1 to 3 wherein the insecticidally effective oil is vegetable oil.
6. An insecticidal or insect repellant composition as claimed in any one of claims 1 to 5 wherein the composition is an emulsion with water.
7. A method of controlling or killing insects comprising exposing an insect population to a composition including Tasmannia stipitata extract and an insecticidally effective oil selected from the group consisting of at least one petroleum oil, at least one vegetable oil. at least one fish oil and mixtures thereof.
8. A method as in claim 7 wherein the composition is applied to the insect population by spraying.
9. A method as in claim 7 or claim 8 wherein the insect population is resident on a plant or an animal.
10. A method as in claim 9 wherein the insect population is resident on a plant.
11. A method as in claimed in any one of claims 7 to 10 wherein
Tasmannia stipitata extract is included in the composition in an amount of from about 0.01% to about 1.25% w/v, preferably 0.02% to 1.0% w/v, most preferably 0.03% to 0.125% w/v.
12. A method as claimed in any one of claim 7 to 11 wherein the insecticidally effective oil is included in the composition in an amount of from about 0.25% to about 1.5% w/v, preferably 0.5% to 1.0% w/v.
13. A method as claimed in any one of claim 7 to 12 wherein the insecticidally effective oil is petroleum oil.
14. A method as claimed in any one of claims 7 to 12 wherein the insecticidally effective oil is vegetable oil.
15. A method as claimed in any one of claims 7 to 14 wherein the composition is an emulsion with water.
16. Use of Tasmannia stipitata extract to enhance the insecticidal activity of an oil selected from the group consisting of at least one petroleum oil, at least one vegetable oil, at least one fish oil and mixtures thereof.
17. The use of claim 16 wherein Tasmannia stipitata extract is included in the composition in an amount of from about 0.01% to about 1.25% w/v, preferably 0.02% to 1.0% w/v, most preferably 0.03% to 0.125% w/v.
18. The use of claim 16 or claim 17 wherein the insecticidally effective oil is included in the composition in an amount of from about 0.25% to about 1.5% w/v, preferably 0.5% to 1.0% w/v.
19. The use as claimed in any one of claim 16 to 18 wherein the insecticidally effective oil is petroleum oil.
Substitute Sheet
20. The use as claimed in any one of claims 16 to 18 wherein the insecticidally effective oil is vegetable oil.
21. The use as claimed in any one of claims 16 to 20 wherein the composition is an emulsion with water.
Substitute Sheet (Rule 26) RO/AU
EP00954174A 1999-08-26 2000-08-28 Insecticidal composition Withdrawn EP1124425A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AUPQ2469A AUPQ246999A0 (en) 1999-08-26 1999-08-26 Insecticidal composition
AUPP246999 1999-08-26
PCT/AU2000/001018 WO2001015534A1 (en) 1999-08-26 2000-08-28 Insecticidal composition

Publications (2)

Publication Number Publication Date
EP1124425A1 EP1124425A1 (en) 2001-08-22
EP1124425A4 true EP1124425A4 (en) 2002-08-21

Family

ID=3816642

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00954174A Withdrawn EP1124425A4 (en) 1999-08-26 2000-08-28 Insecticidal composition

Country Status (5)

Country Link
EP (1) EP1124425A4 (en)
JP (1) JP2003508414A (en)
AU (1) AUPQ246999A0 (en)
CA (1) CA2348976A1 (en)
WO (1) WO2001015534A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030008926A1 (en) * 1997-04-30 2003-01-09 Mcpartland Tor Ant spray containing D-limonene and methods of making and using same
US6663876B2 (en) 2002-04-29 2003-12-16 Piedmont Pharmaceuticals, Llc Methods and compositions for treating ectoparasite infestation
EP2111110B1 (en) * 2007-01-22 2017-10-11 Archer-Daniels-Midland Company Water dispersible compositions comprising a naturally occurring nematicide, lecithin, and a co-surfactant
WO2009065239A1 (en) * 2007-11-19 2009-05-28 Givaudan Sa Compositions and their use
BR102012025669A2 (en) * 2012-10-08 2014-10-14 Sergio Pereira Da Conceicao Silva HEALTH ANT FIGHTING PROCESS, APPLICATION PROCESS AND INSECTICIDE FORMULATION AND PROCEDURE

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
No further relevant documents disclosed *

Also Published As

Publication number Publication date
CA2348976A1 (en) 2001-03-08
WO2001015534A1 (en) 2001-03-08
JP2003508414A (en) 2003-03-04
EP1124425A1 (en) 2001-08-22
AUPQ246999A0 (en) 1999-09-16

Similar Documents

Publication Publication Date Title
Weinzierl Botanical insecticides, soaps, and oils
Moore et al. Plant-based insect repellents
US20030060379A1 (en) Pesticides
EP2009994A2 (en) Biorational repellents obtained from terpenoids and methods related thereto
Murugesan et al. Identification insecticidal properties in common weed-Lantana camara Linn. by gas chromatography and mass spectrum (GC-MS-MS)
Kendra et al. Temporal analysis of sesquiterpene emissions from manuka and phoebe oil lures and efficacy for attraction of Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae)
Mohamad et al. The susceptibility of aphids, Aphis gossypii Glover to lauric acid based natural pesticide
Rochat et al. Male aggregation pheromone of date palm fruit stalk borer Oryctes elegans
Mona Insecticidal potential of cardamom and clove extracts on adult red palm weevil Rhynchophorus ferrugineus
US20080249186A1 (en) Fungicidal Composition
Poland et al. Development of an improved attractive lure for the pine shoot beetle, Tomicus piniperda (Coleoptera: Scolytidae).
KR100341056B1 (en) Natural mosquito repellents
WO2001015534A1 (en) Insecticidal composition
Baker et al. Citronella & citronella oil profile
Fogel Insect mycophagy: a preliminary bibliography
JP4630062B2 (en) Eremophilon and eremophyllone derivatives for pest control
EP1221854B1 (en) Vetiver oil extracts as termite repellent and toxicant
DE102004004900B4 (en) Use of a composition as an attractant for Garden Scarlet Beetle (Phyllopertha horticola)
Sharaby et al. Possibility using camphene as biorational insecticide against the red palm weevil Rhynchophorus ferrugineus (Coleoptera: Curculionedae)
Rongai et al. Vegetable oil formulation as biopesticide to control California red scale (Aonidiella aurantii Maskell)
AU6672700A (en) Insecticidal composition
Mead Acaricidal activity of essential oil of lemongrass, Chymbopogon citratus (DC.) Stapf against Tetranychus urticae Koch
Singh et al. Biochemical pesticides
Uniyal et al. Larvicidal and oviposition deterrent activity of twenty three essential oils against Aedes aegypti
Krall et al. Chemoecological studies reveal causes for increased population densities of Zonocerus (Orth.: Pyrgomorphidae) and offer new means for management

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20010511

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

AX Request for extension of the european patent

Free format text: AL;LT;LV;MK;RO;SI

A4 Supplementary search report drawn up and despatched

Effective date: 20020709

AK Designated contracting states

Kind code of ref document: A4

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

RIC1 Information provided on ipc code assigned before grant

Free format text: 7A 01N 65/00 A, 7A 01N 63/02 -, 7A 01N 61/02 -

17Q First examination report despatched

Effective date: 20021008

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20030303