EP0573571A1

EP0573571A1 - Pneumatic controller for analyzer transport device

Info

Publication number: EP0573571A1
Application number: EP92907724A
Authority: EP
Inventors: Thomas B. Green; Gregory G. O'neil
Original assignee: Tekmar Co
Current assignee: Teledyne Tekmar Co
Priority date: 1991-03-01
Filing date: 1992-02-28
Publication date: 1993-12-15
Also published as: WO1992015875A1; AU1459592A

Abstract

Le dispositif de transport (2) d'échantillons est destiné à être utilisé dans l'analyse d'échantillons selon la technique d'analyse de l'espace libre. Le dispositif comporte un plateau cylindrique (4) doté de chambres à épaulement (16) destiné à recevoir des flacons d'échantillon (20). On chauffe et on fait tourner le plateau (4) d'un premier point de transport (86) de flacon jusqu'à un point d'échantillonnage (118), puis en arrière afin d'éjecter le flacon d'échantillon (20). Le mouvement vertical des flacons d'échantillons (20) s'effectue au moyen de tiges élévatrices (88, 104 et 120) reliées à des vis de mise à niveau (90, 106 et 122) montées au-dessous du plateau cylindrique (4). On améliore la sensibilité, la précision et l'exactitude de l'instrument d'analyse recevant un mélange de gaz et d'espace libre d'échantillon provenant du flacon d'échantillon (20) en positionnant la première unité de commande d'écoulement (148) en amont du régulateur de pression (158), dans la boucle de purge de l'aiguille et de pressurisation du flacon. Ladite boucle est dotée d'une alimentation en gaz (142) indépendante de l'alimentation en gaz destinée au système de purge de l'instrument d'analyse.The sample transport device (2) is intended for use in the analysis of samples according to the free space analysis technique. The device comprises a cylindrical plate (4) provided with shoulder chambers (16) intended to receive sample bottles (20). The tray (4) is heated and rotated from a first vial transport point (86) to a sampling point (118), then backwards in order to eject the sample vial (20) . The vertical movement of the sample bottles (20) takes place by means of lifting rods (88, 104 and 120) connected to leveling screws (90, 106 and 122) mounted below the cylindrical plate (4 ). The sensitivity, precision and accuracy of the analytical instrument receiving a mixture of gas and free sample space from the sample bottle (20) is improved by positioning the first flow control unit (148) upstream of the pressure regulator (158), in the needle purge and pressurization loop of the bottle. Said loop is provided with a gas supply (142) independent of the gas supply intended for the purge system of the analysis instrument.

Description

PNEUMATIC CONTROLLER FOR ANALYZER TR.ANSPORT DEVICE

This application is a continuation-in-part of application Serial No. 487,583, "Analyzer Transport Device", filed March 2, 1990, which was assigned to the assignee of this application. Field of the Invention

The invention relates to devices for trans¬ porting large numbers of samples to a sampling site preparatory to analysis of those samples. Background of the Invention Head space analysis techniques are employed to analyze for volatile components in largely non¬ volatile mixtures. For example, this analytical technique is used in determining the amount of alcohol in a known quantity of blood. The technique is also used for analyses of volatile components in other body fluids. Further applications include analysis of trace organic compounds in water samples, testing for the presence of solvents in drugs, for solvents or monomers in polymers, for fragrances in toiletries, for flavors or aromas in foods, and the like. It is desirable in these applications to have a transport device capable of transporting samples automatically at high rates to the analyzing instrument. It is also desirable for the transport device to be able to be programmed to automatically repeat the sampling of one or more vials one or a number of times. This feature is useful for improving the precision of the analysis. The amount of volatile components in the gaseous headspace portion over a liquid sample in a closed vial is known to vary with the temperature of the liquid sample. Therefore, it is very important to maintain the temperature of the liquid sample within a very narrow range in the transport prior to analysis. Further, because the actual quantity of material in the headspace over a liquid sample is very small, any contamination from outside sources would substantially alter the analysis of the headspace gases. Therefore, the risk of outside contamination must be minimized during the sampling process. Also, because sample vials are made in various sizes, it is desirable that the transport device be able to accommodate different sizes. Summary of the Invention

It is therefore an object of the invention to provide a transport device which is readily auto¬ mated and capable of running large numbers of indi¬ vidual samples. It is another object of the invention to provide a transport device capable of accurately regulating the temperature of samples stored therein. It is yet another object of the invention to provide a transport device capable of performing repeated analyses on a single group of samples.

It is yet a further object of the invention to provide a transport device capable of accepting and sampling vials of different sizes. It is yet a further object of the invention to provide a transport device which transfers sample to an analyzing instrument such as a gas chromatograph in a manner which produces analyses of improved sensitivity, precision, and accuracy. Further objects and advantages of the invention will become evident from the description which follows.

The invention relates to a sample vial transport device having a rotatable heated platen with a plurality of chambers to hold sample vials. The sample vials are loaded into the chambers by a vial transport which conveys the sample vials from a point above the individual chamber. The platen therefore does not have to move axially to input a vial or to sample the vial contents, as discussed below. This significantly reduces the complexity of the vial transport mechanism. Vials from which samples have already been taken are ejected from the individual chamber by reversing the operation of the vial trans¬ port.

Sampling of the contents of the sample vial is done at a point removed from the vial inlet point. The sample vial is rotated toward the sampling point, at which time the sample vial is brought into contact with a needle by mating means. The needle extracts at least a portion of the contents of the vial for sampling by puncturing a septum in the cap of the vial.

The platen is preferably heated electrical¬ ly. Electrical heating is preferred over oil bath heating, which tends to introduce trace materials attributable to the oil into the analysis instrument and thereby alter the analysis results. Further, oil vapors condense on mechanisms and hold dirt. The oil baths need to be constantly stirred to maintain temperature uniformity, and they pose a greater safety hazard to the operator.

The vial from which a sample has been extracted then continues back to the inlet point within the chamber. At that time, it is either ejected from the platen by the vial transport or alternatively remains in the chamber for an additional rotation and sampling operation. The transport device chambers are also capable of holding liner sleeves, or inserts, which permit sampling of different size vials. Typically, the vials utilized have volumes of 5ml, 10ml or 20ml.

In the preferred embodiment of the inven- tion, the needle is stationary and positioned above the platen and a second vial transport is utilized as the mating means to push the sample vial upward from the bottom of the chamber to cause puncturing of the septum with the needle. However, the needle can be movable to puncture the septum as an alternative embodiment.

The rate of transport of gaseous components from the liquid to the headspace of the vial is a function of the mean path length the components must travel through the liquid to reach the headspace. It has been found that mixing the vial contents dramat¬ ically reduces the mean path length and thus improves the transport rate. To facilitate the mixing, the invention includes a mixing device having a vertically displaceable rod positioned below the platen which rises through the bottom of the platen to contact the sample vial bottom in a chamber between the inlet point and the sampling point. The rod is preferably connected to a DC solenoid which is repeatedly ener- gized for short periods to cause the rod to pulse and thereby shake the sample vial in an up and down motion within the chamber. This mixing motion aids in equilibrating the concentration of the material to be analyzed in both the liquid and gas phases within the sample vial.

It has recently been found that the sensitivity, accuracy, and precision of the analyzing instrument receiving sample from the transport device is improved by modifying the relationship between the pressure controller and flow controller in the vial pressurization loop. Placing the flow controller upstream of the pressure controller results in a constant flow rate through this loop regardless of the pressure set at the pressure controller. This substantially eliminates carryover effects due to residual sample which is incompletely purged from the transfer lines.

Further objects, advantages and features of the invention will become apparent upon review of the detailed description of the invention and the drawings in which: Brief Description of the Drawings

Fig. 1 is a side view of the transport device, taken in an axial cross sectional view through the unit with certain features out of position to aid in understanding the invention. Fig. 2 is a side view of the device, taken with a different cross section, and showing all elevator rods in the retracted position. Fig. 3 is a schematic of the carrier gas and sample flow patterns.

Fig. 4 is a top view of the transport device. Fig. 5 is a schematic of the carrier gas and sample flow patterns modified to show a different arrangement of pressure and flow controllers from that depicted in Fig. 3.

Detailed Description of the Invention The invention in its broader aspects relates to a transport device for conveying sample vials to a sampling location for effecting withdrawal of at least a portion of the vial contents for analysis thereof, comprising a platen which is rotatable around a central axis having a plurality of counterbored chambers, a heater for the platen, a first vial transport which operates to convey a vial into a chamber from a point above the chamber and the reverse, a needle for extracting at least a portion of the vial contents from the vial, and mating means for bringing the needle into contact with the vial con¬ tents to allow extraction thereof.

In referring to the drawings. Fig. l shows the sample transport 2 having a platen 4. The platen 4 is a cylindrical block made of heat-conducting material, preferably aluminum, which is manufactured preferably by combining the throughbored block 8 with the bored plate 10 and insulation plate 12. The throughbored holes in block 8 run axially through the block and are positioned near the periphery of the block. These holes mate with the bored holes in plate io, the holes having a smaller diameter than those in block 8. Mating is facilitated by the use of dowel pins 14 in the bored plate 10 which key into mating holes in the block 8. The mating of throughbored block 8 with bored plate 10 creates individual cham- bers 16 for holding a sampie vial 20 having a septum 22 within cap 23. Alternatively, a single block can be counterbored to form the same chambers as are created by combining block 8 with plate 10. However, the preferred embodiment is easier to manufacture and therefore of lower cost.

Below insulation plate 12 is positioned an encoder wheel 24. This wheel has a series of slots 26, an example of which are shown in Fig. 4, which are read by a photodiode transmissive switch assembly 28 to thereby provide a means both for determining the position of the platen 4 and for centering the cham- bers 16 over the various mechanisms, to be described in detail below. The transmissive. switch 28 has a light generating element and a light detecting ele- ent, and the encoder wheel rotates between these two elements. Position is determined by the number and location on the encoder wheel 24 of slots passing light through to the detecting element at any one time. Below the encoder wheel 24 is a support plate 30. Plate 12 is preferably manufactured from a machineable glass fiber, one example of which is known as MARINITE I. Ml-iRINITE I, manufactured by Johns- Manville Corp., Denver, CO is comprised of calcium silicate with inert filters and reinforcing agents. Alternatively, other materials meeting the require¬ ments of machineability and heat insulative ability may be used. For purposes of discussion, the platen 4 will include the throughbored block 8, bored plate 10 insulation plate 12 and support plate 30. The insu¬ lation plate 12, support plate 30 and the encoder wheel 24 are bored to match the borings in plate 10. The platen 4 with encoder wheel 24 is secured to the guide track 36 through screws 38 and spacers 40, several of which being depicted in Figs. 1 and 2. Mating is facilitated by matching dowel pins 42 (only one shown) with mating holes in bored plate 10, insulation plate 12, encoder wheel 24 and support plate 30. The guide track 36 in turn is supported on a ball bearing cage assembly 50. The cage assembly 50 is retained in position below the guide track 36 by the inside shoulder of platen gear 52, which is secured to the bottom side of guide track 36. The bearings comprising the ball bearing cage assembly 50 rest on a thrust washer 54, made preferably from hardened tool steel, which is positioned onto the bas plate 56 and maintained in position by pins (no shown) driven into the base plate 56 adjacent th outside diameter of the thrust washer 54. Alterna- tively, a channel corresponding to the diameter and thickness of the thrust washer 54 could be cut into the base plate 56 and the washer positioned therein. However, this operation requires additional machining and is not necessary to the effective operation of the sample transport.

The guide track 36, and therefore the platen 4, is caused to align properly around a single axis during rotation by the presence of roller wheels 60 mounted onto the base plate 56 by bolt and sleeve assemblies 64. The roller wheel 60 mates with the edge of the guide track 36 along its periphery to maintain the proper orientation. The platen 4 is caused to rotate by actuation of drive motor 66 having a drive gear 68 attached thereto which cooperates with the teeth on platen gear 52.

The throughbored block 8 and bored plate 10 are heated preferably by cartridge heaters 76. The temperature of the block 8 and bored plate 10 is measured by temperature measurement probes 78. These probes preferably are thermocouples, but may be resistance temperature devices or other like device. The cartridge heaters 76 may be easily removed for servicing by removal of an epoxy plug 80 secured to the platen 4 by platen bolt 82, which is shown in Fig. 2.

Sample vials 20 are lowered into the platen 4 and ejected therefrom by a first vial transport 86 consisting of an elevator rod 88 connected to a level winding screw 90 by screw follower 92. The elevator rod 88 is displaced vertically by rotating level winding screw 90 through use of transport motor 94. The elevator rod 88 contacts the bottom of sample vial 20 by travelling upward through aperture 100, which is of smaller diameter than chamber 16. The aperture 100 is bored through bored plate 10, insulation plate 12, encoder wheel 24 and support plate 30. The extent of travel of the elevator rod 88 is regulated by lower limit switch 96 and upper limit switches 97, 98 and 99. Switch 97 stops the upward travel of the elevator rod 88 when the sample vial is to be manually loaded or unloaded. When the vials are to be automatically loaded and unloaded, switches 98 and 99 are used. Switch 98 stops the elevator rod 88 at the auto load point, and switch 99 is for the auto unload, or eject, point.

The sample vial 20 shown in the center chamber position in Fig. 1 is mixed by the mixer device 102, consisting of an elevator rod 104 which rises vertically through aperture 100 by rotation of level winding screw 106 connected to the elevator rod 104 by screw follower 108. Rotation is effected by actuation of mixer motor 109. The DC solenoid 110 located on the screw follower 108 and connected to the elevator rod 104 pulses the rod to provide the neces¬ sary mixing. The solenoid 111 is supplied with a variable voltage input which varies the power supplied to the elevator rod 104. The higher the power, the further the vial 20 rises in the chamber 16 in response to the pulse. This variable voltage feature allows varying of the intensity of mixing.

As discussed above, the transport device 2 as presently configured is capable of accepting vials of 5, 10 and 20 ml volumes. The figures show the 20 ml vials. The smaller vials require liner sleeves, or inserts (not shown) , which support the cap 23 and maintain the septum 22 uniformly near the top of the throughbored block 8. The inserts also have a hole in the bottom through which the elevator rods 104 and 88 travel. Because the caps of the different-sized vials will all be at approximately the same height in the chamber 16, it follows that the bottoms of the vials will rest different distances above the bottom of the chamber 16. During mixing, the elevator rod 104 raises the vial only about 1/16 inch above a rest position. Therefore, with different size vials, the elevator rod 104 must rise different lengths to contact and mix the vials. The limit switches Ilia, b, c, d indicate the elevator rod 104 rest position, and mixing points for the 20ml, 10ml and 5ml vials, respectively. The sample vial 20 in the right chamber is shown in Fig. 1 to be proximate to the needle assembly 112 and is in position for sampling. The vial 20 is raised from a rest position to puncturing contact of the septum 22 with needle 114 by a second vial trans- port 118, which serves as the mating means to bring the needle into contact with the vial contents. The second vial transport consists of an elevator rod 120 terminating with a rod tip 121 which is raised verti¬ cally to contact the bottom of the sample vial 20 (or insert supporting a sample vial) by rotation of level winding screw 122 connected to the elevator rod 120 by screw follower 124. The rod tip has a larger diameter than the hole bored into the bottom of a vial insert to raise the combination of vial and insert. Where the 20ml vial is used, the rod tip 121 contacts the bottom of vial 20 directly. Rotation of the level winding screw is effected by actuating transport motor 126. After sampling is completed, the sample vial 20 is returned into the chamber 16 by reverse movement of the elevator rod 120. The septum 22 is freed from needle 114 with the assistance of a wiper plate 128 which pushes against the sample vial cap 23 via spring 130 loaded against needle flange 132. The wiper plate 128 is maintained in position relative to the needle 114 by means of guide rods 134. The extent of travel of the elevator rod 120 is regulated by lower limit switch 136 and upper limit switch 138.

Fig. 2 shows the sample transport 2 with all elevator rods in the fully retracted position. It can be seen that the needle 114 is positioned directly above the platen 4. The close positioning of the needle assembly 112 to the top of the platen 4 permits the sample vials 20 to be sampled while only being lifted a short distance inside the chamber. This close arrangement of needle assembly 112 to the top of the platen 4 minimizes temperature fluctuation within the individual sample vials as the sample is with¬ drawn.

The various components of the platen are secured to each other and to the epoxy plug 80 by the platen bolt 82. Fig. 4 shows the top view of the transport device and the relationship of the components. The platen 4 has positioned near its outside circumference a plurality of chambers 16. Inside the circumference of the chambers are located the temperature measure- ent probes 78. Near the axis of the platen 4 are the cartridge heaters 76. The various components com¬ prising the platen 4 are secured by the platen bolt 82. Below the platen 4 at the nine o'clock position is the first vial transport 86. The tip of the elevator rod 88 can be seen in position ready to rise through aperture 100. At the twelve o'clock position below the platen 4 is the mixer device 102, with the tip of elevator rod 104 appearing in aperture 100. At the three o'clock position below the platen is located the second vial transport 118, with the tip of eleva¬ tor rod 20 showing. In phantom is depicted the needle flange 132 which is located over the chamber corre¬ sponding to the second vial transport 118.

The sampling of the headspace contents operates as follows. The headspace contents within a sample vial 20 are removed from the sample vial 20 and conducted to a gas chromatograph (G.C.) for analysis by the following procedure. Fig. 3 is a schematic showing one embodiment of the gas flow pattern into the sample transport, depicted in part as the heated zone 140 and separate platen heated zone 141, out the sample transport and then to the gas chromatograph. Zone 141 is the platen 4 itself. The heating of this zone 141 has been generally discussed and will be described in more detail below. The zone 140 is heated to prevent condensation in the lines between the needle 114 and the gas chromatograph. The temperature in this zone is typically set about 10° to about 25"C higher than the platen 4 temperature. Carrier gas, typically helium, enters gas inlet 142 from a tank controlled with a pressure regulator (not shown) . The gas supply is split at T-connection 144. The tubing and connections through 5 which the carrier gas flows are made from nickel alloy, copper, stainless steel, or other material which does not evolve any compounds which might affect the G.C. analysis and which is not permeable to compounds in the air which might diffuse through the 10 material and be carried to the G.C. Carrier gas flows through first line 146 at a pressure set at the tank regulator, typically about 60 psi, to a gas chromato¬ graph flow controller 148. The controller 148 is connected in line to pressure gauge 150. Carrier gas 15 then flows into six port valve 152 having ports A, B, C, D, E and F. The carrier gas flowing through the first line 146 ultimately enters six port valve 152 at port C, exits through port D and continues through the heated transfer line 154 into the gas chromatograph. 2₀. This flow of gas is always on to constantly purge the gas chromatograph. This portion of the gas transport system including the lines, valves, regulators and the connections and fittings between first line 146 and heated transfer line 154 is the analyzing instrument 5 purge system.

The remaining flow of carrier gas after splitting passes through second line 156. The carrier gas flowing through this second line is used to purge the needle 114 and to pressurize the sample vial 20 after the septum 22 has been punctured by needle 114. This portion of the gas transport system including the lines, valves, regulators and the connections and fittings between second line 156 and needle 114 is the needle purge and vial pressurization loop. Pressure is adjusted by regulator 158 within a range of about 5 to about 30 psi, depending on the application. The flow is regulated by flow controller 160. Carrier gas flow through the needle 114 is turned on and off by a solenoid operated pressurization valve 162. The flow controller 160 is set at a level sufficient to pres¬ surize the sample vial in a reasonable time, but not so high as to waste excessive amounts of carrier gas as the needle is being purged. Typically, the valve 162 is open to constantly purge the needle 114. With the pressurization valve 162 on, and vent valve 164 off, carrier gas flows through T-connection 168 to inlet line 170 and then into the six port valve 152 at port A. The carrier gas flows from port A into port B, through sample loop 172, into port E, out port F and through the needle 114 into the sample vial 20. After a programmed amount of time sufficient to pressurize the vial 20 to at least the same pressure as that set at the regulator 158, the pressurization valve 162 is turned off and the pressure within the lines from that valve to the sample vial is allowed to equilibrate.

After a preprogrammed equilibration time of several seconds to several minutes, the vent valve 164 is opened to allow the vial gases, consisting of carrier gas and headspace contents, to vent. Carrier gas with the headspace contents flows back through needle 114, into the six port valve at port F and into the sample loop 172 and out port A toward the vent valve 164. After an additional programmed time, the vent valve 164 is closed and the six port valve 152 is cycled. The carrier gas in the first line 146, which remained on and flowed out port D to the gas chromato¬ graph, is now caused to flow through port C to port B after the cycling. The carrier gas flows into the sample loop 172 and flushes the contents into port E which is now connected with port D. This allows the materials to flow into the gas chromatograph through heated transfer line 154. After the sample contents have been injected into the gas chromatograph, the six port valve 152 returns to its home position, the sample vial 20 is removed from needle 114 and the pressurization valve 162 is again opened to purge the needle of any residual headspace material. When a new sample vial 20 is brought in contact with the needle, the procedure repeats. Surprising improvements in sensitivity, precision, and accuracy of the analyzing instrument, such as a gas chromatograph, have been obtained by changing the arrangement of pressure regulator 158 and flow controller 160 from that shown in Fig. 3 to that depicted in Fig. 5, which shows a second, preferred, embodiment of the gas flow pattern. The sensitivity of the instrument relates as to the minimum amount of sample which can be detected. The precision of the instrument relates to the reproducibility of results after multiple runs of the same material. The higher the precision, the smaller the standard deviation over a series of runs for a particular sample. The accuracy of the instrument directly relates to the extent of agreement between the analyzed physical properties for a particular sample and the actual physical properties of the sample. The closer the agreement, the more accurate the instrument.

In the original configuration in Fig. 3, the pressure regulator 158 was positioned upstream of flow controller 160 in the loop used to purge the needle

114 and to pressurize the sample vial 20. Both the pressure and flow rate of the carrier gas flowing toward needle 114 was adjustable in this configuration. However, it is known that the flow of a gas exiting the flow controller 160 varies directly with the pressure of the gas at the flow controller 160. Thus, if the flow controller 160 in Fig. 3 is nominally set at a flow rate of 10 cubic centimeters

(cc) per minute at a pressure of 20 psig, the flow will decrease when the pressure is decreased by adjusting pressure regulator 158, even though no subsequent adjustment was made at the flow controller

160.

As a result of the pressure decrease, the flow of carrier gas through the inlet line 170, six port valve 152, sample loop 172, needle 114 and ultimately into the headspace of sample vial 20 decreases. In like manner, an increase in pressure set at regulator 158 in Fig. 3 results in an increase in flow over that originally set at flow controller 160.

The precision, accuracy and sensitivity of analysis of a specific run by the gas chromatograph is adversely affected by the variation of the concentration of sample to be analyzed in the carrier gas, and by the presence of trace amounts of residual sample from other runs. Where the flow rate changes as a result of pressure regulator change, the concentration of sample in carrier gas in the sample vial 20 and ultimately in the gas chromatograph also changes due to the variation in total volume of carrier gas added to the sample vial 20 per unit time. Further, a decrease in the flow rate due to a decrease in pressure set at the pressure regulator 158 opens the possibility that the amount of carrier gas purging needle 114 will be insufficient to completely remove any trace residual sample from an earlier run. The preferred second embodiment as depicted in Fig. 5 involves placing the flow controller 160 upstream of pressure regulator 158. Further, T-connection 168 is positioned inside heated zone 140a. The temperature in heated zone 140a is set according to the same parameters previously disclosed for heated zone 140 in the first embodiment. Finally, the gas supplied to the analyzing instrument purge system through gas inlet 142 in the second embodiment is independent of the gas supplied to the needle purge and vial pressurization loop through gas inlet 180. Flow controller 160 is supplied with gas from gas inlet 180 through line 182. Because the gas pressures upstream of flow controllers 148 and 160 are typically maintained at a constant value by conventional two-stage regulators (not shown) at the carrier gas supply such as a gas bottle, the flow rates within the analyzing instrument purge system and the needle purge and vial pressurization loop are only changed by adjustment of the flow controllers 148 and 160 respectively. Adjustment of the pressure by regulator 158 does not vary the flow in the needle purge and vial pressurization loop. Therefore, the flow rate during the needle purge step remains constant until the source of the carrier gas is exhausted. Further, the amount of gas supplied to the headspace in sample vial 20 during vial pressurization is constant per unit time regardless of the desired pressure in sample vial 20 as set at pressure regulator 158. Thus, the amount of carrier gas flowing from flow controller 160 through the intermediate connections to needle 114 is constant per unit time during the needle purge step, which substantially eliminates the risk of residual sample from previous runs affecting analysis of the particular sample due to insufficient purge flow.

Changing the arrangement of the pressurization gas control pneumatics, namely with the flow controller 160 positioned upstream from the pressure regulator 158, provides the capability of sweeping carryover from the sample line with a constant and optimal volume of sweep gas regardless of as chromatograph run time. For instance, if it is determined empirically that a volume of 800 cubic centimeters (cc) of gas must sweep from flow controller 160, through inlet line 170, through six-port valve 152 at ports A and F, and out needle 114 in order to eliminate carryover, the flow controller 160 may be adjusted to the proper rate to achieve this goal. Thus, if 800 cc of gas is required, and the gas chromatograph run time is 10 minutes, a needle purge flow rate of 80 cc/min. will provide the necessary purge volume. A constant and sufficient sweep volume improves both the precision and accuracy of the gas chromatograph or other analyzing instrument through the elimination or reduction of carryover.

In addition, placing the pressure regulator 158 downstream of flow controller 160 permits independent control of the amount of gas that is added to the vial during vial pressurization. This gas can be the same or different from the gas supplied at gas inlet 142 for the analyzing instrument purge system. This arrangement permits utilizing the pressurization regulator 158 as a dilution control device. In general, the higher the pressurization setting on the pressure regulator 158, the greater the amount of pressurization gas introduced into the headspace of the vial. The increased pressure produces a dilution effect on the headspace sample which may be demonstrated by analysis. As the pressure increases, assuming a fixed phase? ratio (volume of headspace gas/volume of liquid or solid in vial) , the amount of sample as determined by area count from the detection system decreases. The decreased area count indicates a dilution of the headspace sample. Further, providing independent gas sources through gas inlets 142 and 180 permits the pressurization of vial 20 and subsequent sweeping of the needle purge loop with a gas other than the carrier gas supplied through gas inlet 142. Thus, enhanced removal of headspace sample from vial 20 may be obtained by pressurizing the vial through gas inlet 180 with a gas such as carbon dioxide. This gas may be impractical or uneconomical for use as a carrier gas for conveying sample along heated transfer line 154 to the gas chromatograph. The modification disclosed above and in Fig. 5 permits a separate gas to be used as the carrier gas, supplied through gas inlet 142. This arrangement permits increased flexibility in matching the vial headspace contents to the vial pressurization gas to maximize sample removal while allowing for independent selection of the optimum carrier gas.

In supplying sample to an analyzing instrument such as a gas chromatograph from a transport device such as an autosampler, it is desirable to independently control three key variables — the flow rate or back pressure at the column of the gas chromatograph, the pressure exerted on the vial by the pressurization gas, and the standby flow rate to purge the transfer lines in the transport device between runs. The analyzer transport device of this invention accomplishes independent control over these variables.

The analyzer transport device described above is used preferably for preparing samples for analysis using the headspace analysis technique, wherein the gaseous sample is heated to a set tempera¬ ture and the contents sent to a gas chromatograph. The operable temperature range is typically room temperature plus 10*C to about 200^βC. The above transport device can be automatically loaded and unloaded, and large numbers of samples can be pro¬ cessed without constant operator supervision. The electrically powered functions of the device are controlled through a keypad or other similar type of control unit (not shown) which, among other functions, adjusts the sample vial processing rate through the transport device, adjusts temperature, severity and time of mixing, sampling order by use of the encoder wheel in combination with the photodiode transmissive switch assembly, and number of repetitions of analysis of the headspace contents of a single sample vial.

Thus it is apparent that there has been provided in accordance with the invention, a sample transport device that fully satisfies the objects, aims, and advantages set forth above. While the invention has been described in conjunction with specific embodiments thereof, it is evident that many alternatives, modifications, and variations will be apparent to those skilled in the art in light of the foregoing description. Accordingly, it is intended to embrace all such alternatives, modi ications, and variations as fall within the spirit and broad scope of the appended claims.

Claims

What is claimed is: 1. A transport device for conveying a vial to a sampling point, removing sample from the headspace of the vial and transferring the sample to an analyzing - instrument, comprising: a platen having a plurality of counterbored chambers rotatable around a central axis; a heater for said platen; a first vial transport operating to convey a vial into a chamber from a point above said chamber, and the reverse; a needle for extracting at least a portion of the headspace sample from said vial; mating means for bringing said needle into contact with said headspace sample to allow extraction thereof; and a sample transport system to inject gas into the headspace of said vial via said needle and to convey said headspace sample mixed with said gas to 0 the analyzing instrument, said sample transport system having a needle purge and vial pressurization loop with a pressure regulator and flow controller, said flow controller positioned upstream of said pressure regulator.

2. The transport device of claim 1 having an analyzing instrument purge system.

3. The transport device of claim 2 wherein said needle purge and vial pressurization loop operates independently of an analyzing instrument purge system.

4. The transport device of claim 2 having a first gas supply for said needle purge and vial pressurization loop and a second gas supply for said analyzing instrument purge system.

5. The transport device of claim 4 wherein said first gas supply is independent from said second gas supply.

6. The transport device of claim 1 wherein the flow of said gas within said needle purge and vial pressurization loop is unaffected by changes in pressure controlled by said pressure regulator.

7. A method for minimizing fluctuations in flow rate of a gaseous sample mixed with gas input to an analyzing instrument from a transport device having a gaseous sample transport system with a flow controller and a pressure regulator, by positioning said flow controller upstream of said pressure regulator in said gaseous sample transport system.

8. A gaseous sample transport system for injecting gas into the headspace of a pressurizable sample vial having a septum to mix with headspace sample, and subsequently removing at least a portion of the resulting mixture from the sample vial, comprising: a gas source; a six port valve; a gas line connecting said six port valve and said gas source, said gas line having a flow controller and pressure regulator connected in-line for adjusting the flow and pressure, respectively, within said sample transport system, said flow controller positioned upstream of said pressure regulator; a needle connected by a transfer line to said six port valve for piercing the septum of the sample vial, which conducts gas into the headspace of said sample vial and conveys the mixture of said gas and headspace sample through said transfer line to said six port valve; a sample loop connected to said six port valve for receiving said mixture of gas and headspace sample; and a vent valve for releasing said pressure within said sample transport system, thereby causing said mixture of said gas and said headspace sample in said sample vial to flow toward said six port valve and into said sample loop.

9. The system of claim 8 wherein an analyzing instrument purge system is connected to said gas source.

10. The system of claim 9 wherein said purge system is connected to said six port valve.

11. The system of claim 10 wherein said purge system conducts said mixture of said gas and said headspace sample from said sample loop to said analyzing instrument.