EP0374905A1 - Culture medium device and method of preparing the same - Google Patents
Culture medium device and method of preparing the same Download PDFInfo
- Publication number
- EP0374905A1 EP0374905A1 EP19890123596 EP89123596A EP0374905A1 EP 0374905 A1 EP0374905 A1 EP 0374905A1 EP 19890123596 EP19890123596 EP 19890123596 EP 89123596 A EP89123596 A EP 89123596A EP 0374905 A1 EP0374905 A1 EP 0374905A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- sheet
- culture medium
- water repellent
- hydrophilic
- property
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/801—Anerobic cultivation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/805—Test papers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T428/00—Stock material or miscellaneous articles
- Y10T428/13—Hollow or container type article [e.g., tube, vase, etc.]
- Y10T428/1303—Paper containing [e.g., paperboard, cardboard, fiberboard, etc.]
- Y10T428/1307—Bag or tubular film [e.g., pouch, flexible food casing, envelope, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T428/00—Stock material or miscellaneous articles
- Y10T428/31504—Composite [nonstructural laminate]
- Y10T428/31855—Of addition polymer from unsaturated monomers
- Y10T428/3188—Next to cellulosic
- Y10T428/31895—Paper or wood
- Y10T428/31899—Addition polymer of hydrocarbon[s] only
- Y10T428/31902—Monoethylenically unsaturated
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T428/00—Stock material or miscellaneous articles
- Y10T428/31504—Composite [nonstructural laminate]
- Y10T428/31971—Of carbohydrate
- Y10T428/31993—Of paper
Definitions
- a known sheet-form culture medium generally comprises laminated upper and lower sheet members.
- the upper sheet is peeled off at a time just before the usage thereof to drop aseptic water such as distilled water on the upper surface of the lower sheet to wet the same.
- a material or substance is thereafter inoculated to the central portion of the upper surface of the lower sheet by means of a pipette and the upper sheet is then covered over the lower sheet.
- a spreader made of a plastic material is placed on the upper surface of the upper sheet member at a portion corresponding to the location of the inoculated material on the lower sheet.
- a light pressure is then applied to the spreader to uniformly spread the material over substantially the entire upper surface of the lower sheet, whereby the material is cultured.
- Fig. 1 shows a culture medium device in the form of a sheet according to the present invention having a square configuration, for example, having a side length of 50 mm and a thin thickness of 2 mm, for instance.
- the culture medium device 1 comprises an upper sheet 6 having a water repellent property and a lower base sheet 7.
- the upper water repellent sheet 6 is made of a material selected in accordance with the bacteria or microorganism to be treated. For example, with bacteria having an aerobic property, the water repellent sheet 6 is prepared by an oxygen permeable film and with the bacteria of an anaerobic property, the sheet 6 is prepared by an impermeable film.
- the upper water repellent sheet 6 may be thus prepared of a material such as polyethylene or polypropylene having a thickness of about 20 ⁇ .
Landscapes
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A culture medium device (1) in the form of a sheet comprises a base sheet (7) composed of an upper sheet (8) having a hydrophilic property such as filter paper, a lower sheet (9) having a water repellent property covering a lower surface of the upper hydrophilic sheet (8) and a sheet having a water repellent property preferably in the form of a film having a character suitable for bacteria to be treated. A gel agent or gelatinizer containing bacteria culturing nutrient is dispersed on an upper surface of the upper hydrophilic sheet (8) of the base sheet member (7) and the gel agent or gelatinizer is absorbed in the upper hydrophilic sheet (8) and then solidified. The upper water repellent sheet (6) is applied so as to cover the upper surface of the upper hydrophilic sheet (8) of the base sheet (7).
Description
- The present invention relates to a device for culturing microorganisms, and more particularly, to a culture medium in the form of a sheet for culturing adhesive bacteria and dropping bacteria, and a method of preparing the culture medium device.
- A known sheet-form culture medium generally comprises laminated upper and lower sheet members. The upper sheet is peeled off at a time just before the usage thereof to drop aseptic water such as distilled water on the upper surface of the lower sheet to wet the same. A material or substance is thereafter inoculated to the central portion of the upper surface of the lower sheet by means of a pipette and the upper sheet is then covered over the lower sheet. A spreader made of a plastic material is placed on the upper surface of the upper sheet member at a portion corresponding to the location of the inoculated material on the lower sheet. A light pressure is then applied to the spreader to uniformly spread the material over substantially the entire upper surface of the lower sheet, whereby the material is cultured.
- In the preparation of the sheet-form culture medium in accordance with a conventional method described above, it is necessary to peel off the upper sheet and drop the aseptic water on the upper surface of the lower sheet before the usage of the culture medium, so that the aseptic water must be transferred to a portion at which the aseptic water is actually used. In addition, contamination of the environmental atmosphere to the upper surface of the sheet when the aseptic water is dropped on the upper surface of the lower sheet also has to be considered as it requires the sterilization of instruments or the like for every usage thereof. These inconveniences require users to pay close attention to the treatment and operation of the culture medium preparation, therefore making it troublesome and time consuming.
- An object of the present invention is to substantially eliminate defects or drawbacks encountered in the prior art described above and to provide a culture medium device in the form of a sheet and a method of preparing the same, and which is capable of being easily transferred and usable without requiring sterilization.
- This and other objects can be achieved in one aspect according to the present invention by providing a culture medium device in the form of a sheet comprising a base sheet member composed of an upper sheet having a hydrophilic property and a lower sheet having a water repellent property and covering a lower surface of the upper hydrophilic sheet in which a gel agent or gelatinizer containing a bacteria culturing nutrient is dispersed therein, and then solidified, and a sheet member having a water repellent property covered over the upper hydrophilic sheet of the base sheet member.
- In a preferred embodiment, the upper water repellent sheet member is formed of a film member selected in accordance with the bacteria to be treated. The hydrophilic sheet of the base sheet member is formed of a filter paper.
- In another aspect according to the present invention, there is provided a method of preparing a culture medium device in the form of a sheet comprising the steps of preparing a base sheet member composed of an upper sheet having a hydrophilic property and a lower sheet having a water repellent property covering a lower surface of the upper hydrophilic sheet and preparing a sheet member having a water repellent property to be covered over the upper hydrophilic sheet of the base sheet member, preparing a gel agent or gelatinizer containing bacteria culturing nutrient, dispersing and solidifying the gel agent or gelatinizer on an upper surface of the upper hydrophilic sheet of the base sheet member, in the upper hydrophilic sheet, and applying the upper water repellent sheet member so as to cover the upper surface of the upper hydrophilic sheet of the base sheet member.
- According to the present invention of the character described above, the culture medium is prepared by dispersing the gel agent or gelatinizer containing the bacteria culturing nutrient on an upper surface of the hydrophilic sheet having a lower surface covered by the water repellent sheet and then absorbing and solidifying the dispersed gel agent or gelatinizer. The upper surface of the hydrophilic sheet thus prepared is then covered with the water repellent sheet member. Accordingly, the culture medium can be maintained without being dried. The inoculation can be performed immediately after peeling of the water repellent sheet member.
- In the accompanying drawings:
- Fig. 1 is a perspective view of one embodiment of a culture medium device according to the present invention showing a state in which a material has just been inoculated by means of a pipette;
- Fig. 2 is a sectional view taken along the line II-II shown in Fig. 1;
- Fig. 3 is a view, according to a prior art, showing a state similar to that of Fig. 1; and
- Fig. 4 is a perspective view of a conventional culture medium plate on which a spreader is placed.
- For a better understanding of the present invention, a conventional sheet-shaped culture medium will be described hereunder with reference to Figs. 3 and 4 in the preparation thereof.
- Referring to Fig. 3, a culture medium sheet 1 generally comprises an
upper sheet member 2 and alower sheet member 3 which are laminated to each other. Theupper sheet 2 is peeled off at a time just before the usage thereof to drop aseptic water such as distilled water on the upper surface of thelower sheet 3 to wet the same. When bacteria are made to adhere by, for example, pressing an upper surface of alower sheet 3 against a sample or when a material has been inoculated by a pipette, theupper sheet 2 is then covered over thelower sheet 3. After the covering of theupper sheet 2, as shown in Fig. 5, a spreader 5 made of a plastic material is placed on the upper surface of theupper sheet 2 at a portion corresponding to the location of the inoculated material on thelower sheet 3. A light pressure is then applied to the spreader 5 to uniformly spread the material over substantially the entire upper surface of thelower sheet 3, whereby the material is cultured. - However, the preparation of the culture medium sheet on the basis of the conventional method involves the inconveniences or problems described hereinbefore.
- Preferred embodiments according to the present invention conceived by taking the above problems of the prior art into consideration will be described hereunder with reference to Figs. 1 to 3, in which like reference numerals are added to elements or members corresponding to those shown in Figs. 3 and 4.
- Fig. 1 shows a culture medium device in the form of a sheet according to the present invention having a square configuration, for example, having a side length of 50 mm and a thin thickness of 2 mm, for instance. The culture medium device 1 comprises an
upper sheet 6 having a water repellent property and alower base sheet 7. The upperwater repellent sheet 6 is made of a material selected in accordance with the bacteria or microorganism to be treated. For example, with bacteria having an aerobic property, thewater repellent sheet 6 is prepared by an oxygen permeable film and with the bacteria of an anaerobic property, thesheet 6 is prepared by an impermeable film. The upperwater repellent sheet 6 may be thus prepared of a material such as polyethylene or polypropylene having a thickness of about 20 µ. Thelower base sheet 7 is composed of an upper sheet-like filter paper 8 having a hydrophilic property and a lowerwater repellent sheet 9 made of a material such as polyethylene and having a thickness of about 20 µ. The lowerwater repellent sheet 9 is adhered to theupper filter paper 8 for maintaining the wetness of thefilter paper 8 and for reinforcing the same. The upperwater repellent sheet 6 and thelower base sheet 7 are laminated and bonded at one of their ends to allow the upperwater repellent sheet 6 to be opened or rolled up as shown in Fig. 1 with respect to thelower base sheet 7. - In order to prevent the invasion of bacteria or germs in the case where the culture medium device 1 is not used, the culture medium device 1 may be sealed in a bag made of such as a plastic material, or the outer peripheries of the upper
water repellent sheet 6 and the lowerwater repellent sheet 9 may be bonded (fused) to seal them by means of separate tapes. Any other means may be applied to seal the culture medium device 1 to prevent the invasion of germs. - When the aerobic bacteria is treated, it may be preferred to locate an air space between a gelled surface and the upper
water repellent sheet 6 to introduce the air therein. - The
final culture medium 10 for the culture medium device 1 will be completed by preparing a gel agent or gelatinizer formed of sodium alginate of 0.5 to 4.0%, containing a bacteria culturing nutrient in the gel agent for gelatinizer, and dispersing the thus prepared gel agent or gelatinizer into the sterilizedfilter paper 8 in a sterilized state. A sterilized color former ((2,3,5-Triphenyl-2H-tetrazolium chloride (TTC)) may be contained in the alginic acid. The surface of thefilter paper 8 may be held in a sterilized state by absorbing sterilized calcium chloride of 10 to 500 mM and the gel agent or gelatinizer is solidified in thefilter paper 8. As the bacteria culturing nutrient are utilized casein hydrolyzate of 1.5%, soybean pepton of 0.5%, and potassium chloride of 0.5% for the bacteria and, for the Eumycetes, are utilized glucose of 2.0%, yeast extract of 0.2%, magnesium sulfate of 0.05%, casein hydrolyzate of 0.5%, and potassium dihydrogen phosphate of 0.1%. - The culture medium device 1 in the form of a sheet of the character described above will be utilized in the following manner.
- The upper
water repellent sheet 6 is peeled off from thebase sheet 7, and bacteria are made to adhere on thefilter paper 8 by pressing a sample against thefilter paper 8 or by falling dropping bacteria on thefilter paper 8. Thewater repellent sheet 6 is then covered over thefilter paper 8 whereby the material is cultured. In a case of a liquid material, a small amount of the material is dropped thereon. - As described above, the
filter paper 8 of thebase sheet 7 has been made in the sterilized and gel state before the usage of the sheet-like culture medium device 1, so that it is not necessary to wet the filter paper with an aseptic water such as distilled water. The sterilized state of thefilter paper 8 can be maintained without being dried because the upperwater repellent sheet 6 and the lowerwater repellent sheet 9 of thebase sheet 7 are made of polyethylene, for example. - In a modification, the culture medium device 1 may be accommodated in a bag having air-impermeability with a mouth portion sealed by a heat sealing method, for example, to stably maintain the sterilized condition. The bag may be further exposed to γ-rays or ultra violet rays for the sterilization of the culture medium device. The device may be sterilized by the utilization of gas (for example EOG) or by an autoclave treatment.
- In the described embodiment, the filter paper is utilized as a hydrophilic sheet, but a plastic sheet material or ceramic sheet material may be utilized.
- The culture medium device according to the present invention may be utilized for the elapsed time capture of bacteria such as dropping bacteria for the environmental measurement and utilized as a stamp for culturing adhesion bacteria.
- Some concrete examples will be described hereunder in comparison with conventional examples.
- Sodium alginate (30 g) was dissolved in a distilled water (1000 ml) and a sterilization dry culture medium (Trypticase soy Broth; BBL Co. Ltd.: 30 g) was thereafter dissolved to prepare a solution. A qualitative filter paper No. 2 of TOYO ROSHI having a size of 4 x 5 cm and preliminarily lined by a PET film was impregnated in the thus prepared solution. The qualitative filter paper was immediately impregnated into 2% calcium chloride solution after being taken out from the afore-mentioned solution and gelled. The gelled material was then covered with the PET film and sterilized by the autoclave treatment at 121°C for 20 minutes.
- Aseptic water was applied by 1 ml to a Petrifilm SM of 3M firm in accordance with a manual of 3M firm and disposed as it is for 20 minutes.
- Bacteria adhesion tests were carried out with respect to the Example 1 and the Comparative Example 1. The tests were carried out by coating the Escherichia coli of a predetermined amount on a sterilized glass plate and then opening a cover. A sheet-form culture medium and the Petrifilm SM were pressed against the glass plate for about 10 seconds. Thereafter, the culture medium was covered and cultured at 37°C for 24 hr. and the numbers of the cultured colonies were counted. The counting results are shown in the following Table 1.
Table 1 n=10 Number of Colonies Example 1 33 Comparative Example 1 31 - A culture medium was prepared by substantially the same manner as described with respect to the Example 1, sealed in a plastic bag, and then sterilized by the irradiation of γ-rays.
- Trypticase soy Broth (30 g) and Agar (20.0 g) were dissolved in distilled water (1000 ml) into a solution. The solution was sterilized at 121°C for 20 minutes by autoclave treatment. The solution was thereafter shared into sterilization Petri dishes each having a diameter of 50 mm and then solidified therein.
- Dropping bacteria tests were carried out with respect to the Example 2 and the Comparative Example 2 by removing the cover of the culture medium and the cover of the Petri dish to catch and collect the dropping bacteria in a room for 5 minutes. Thereafter, the covers were applied and the cultured colonies were counted and the counting results are obtained as shown in the following Table 2.
Table 2 n=10 Number of Colonies Example 2 13 Comparative Example 2 15 - As described hereinabove, according to the adhesion bacteria tests and the dropping bacteria tests, substantially the identical numbers of the colonies were observed with respect to the Examples and the Comparative Examples. However, as described with respect to the Example 2, when the culture medium is preliminarily prepared, it is not necessary to prepare the distilled water and add the same to the culture medium 20 minutes before each test as is carried out in case of the usage of the Petrifilm. Moreover, it is also not necessary to prepare and sterilize the culture medium for each test as described with respect to the Comparative Example.
Claims (5)
1. A culture medium device in the form of a sheet characterized by comprising:
a base sheet (7) composed of an upper sheet (8) having a hydrophilic property and a lower sheet (9) having a water repellent property and covering a lower surface of said upper hydrophilic sheet in which a gel agent or gelatinizer containing a bacteria culturing nutrient is dispersed in and absorbed by said upper hydrophilic sheet, said gel agent or gelatinizer being then solidified; and
a sheet (6) having a water repellent property to be covered over said upper hydrophilic sheet of the base sheet member (7).
a base sheet (7) composed of an upper sheet (8) having a hydrophilic property and a lower sheet (9) having a water repellent property and covering a lower surface of said upper hydrophilic sheet in which a gel agent or gelatinizer containing a bacteria culturing nutrient is dispersed in and absorbed by said upper hydrophilic sheet, said gel agent or gelatinizer being then solidified; and
a sheet (6) having a water repellent property to be covered over said upper hydrophilic sheet of the base sheet member (7).
2. A culture medium device according to claim 1, wherein said upper hydrophilic sheet (8) of the base sheet member (7) is made of a filter paper.
3. A culture medium device according to claim 1, wherein said upper water repellent sheet (6) is made of an oxygen permeable film when a bacteria to be treated has an aerobic property.
4. A culture medium device according to claim 1, wherein said upper water repellent sheet (6) is made of an impermeable film when a bacteria to be treated has an anaerobic property.
5. A method of preparing a culture medium device in the form of a sheet characterized in that a base sheet (7) composed of an upper sheet (8) having a hydrophilic property and a lower sheet (9) having a water repellent property covering a lower surface of said upper hydrophilic sheet and preparing a sheet having a water repellent property to be covered over said upper hydrophilic sheet of the base sheet, a gel agent or gelatinizer containing bacteria culturing nutrient is prepared, the gel agent or gelatinizer is dispersed on an upper surface of said upper hydrophilic sheet of the base sheet, said gel agent or gelatinizer is absorbed in said upper hydrophilic sheet (8) and then solidified, and said upper water repellent sheet (6) is applied so as to cover the upper surface of said upper hydrophilic sheet of the base sheet.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP63322432A JPH02167067A (en) | 1988-12-21 | 1988-12-21 | Sheet-like culture medium |
JP322432/88 | 1988-12-21 |
Publications (1)
Publication Number | Publication Date |
---|---|
EP0374905A1 true EP0374905A1 (en) | 1990-06-27 |
Family
ID=18143606
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP19890123596 Withdrawn EP0374905A1 (en) | 1988-12-21 | 1989-12-20 | Culture medium device and method of preparing the same |
Country Status (3)
Country | Link |
---|---|
US (1) | US5147801A (en) |
EP (1) | EP0374905A1 (en) |
JP (1) | JPH02167067A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5232838A (en) * | 1991-12-09 | 1993-08-03 | Minnesota Mining And Manufacturing Company | Culture media device and method of use |
US8906645B2 (en) | 2010-12-29 | 2014-12-09 | 3M Innovative Properties Company | Microbial detection article having a water-absorbent filter assembly |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5462860A (en) * | 1994-06-06 | 1995-10-31 | Minnesota Mining And Manufacturing Company | Conditioned culture medium for rapid growth and detection of microbes |
US20050239200A1 (en) * | 2004-04-23 | 2005-10-27 | Beckwith Scott W | Devices for culturing anaerobic microorganisms and methods of using the same |
FR3010716B1 (en) * | 2013-09-16 | 2015-10-09 | Commissariat Energie Atomique | SUBSTRATE FOR SOLIDIFICATION OF SILICON INGOT |
FR3010715B1 (en) * | 2013-09-16 | 2017-03-10 | Commissariat Energie Atomique | LOW PERMEABLE COATING SUBSTRATE FOR SILICON SOLIDIFICATION |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3338794A (en) * | 1964-11-23 | 1967-08-29 | Swift & Co | Culturing anaerobic bacteria |
DE2055741A1 (en) * | 1969-11-14 | 1971-05-19 | National Research Development Corp , London | Microbiological growth tray |
EP0006192A1 (en) * | 1978-06-12 | 1980-01-09 | Roche Diagnostics GmbH | Device and procedure for microbiological work |
WO1982002563A1 (en) * | 1981-01-27 | 1982-08-05 | Minnesota Mining & Mfg | Dry culture media |
EP0146143A2 (en) * | 1983-12-20 | 1985-06-26 | Showa Yakuhin Kako Co., Ltd. | Simplified detection implement |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE739939C (en) * | 1938-07-29 | 1943-10-08 | Schleicher & Schuell Carl | Drawing pad made of pressboard |
FR1032900A (en) * | 1951-02-20 | 1953-07-06 | Elce Soc | Material for various packaging such as boxes, bags and similar items |
FR1306147A (en) * | 1961-09-06 | 1962-10-13 | Coating for cold sealing cellulosic film, paper or other | |
US4587213A (en) * | 1971-09-29 | 1986-05-06 | Malecki George J | Methods and means of determining microorganism population |
-
1988
- 1988-12-21 JP JP63322432A patent/JPH02167067A/en active Pending
-
1989
- 1989-12-19 US US07/452,767 patent/US5147801A/en not_active Expired - Lifetime
- 1989-12-20 EP EP19890123596 patent/EP0374905A1/en not_active Withdrawn
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3338794A (en) * | 1964-11-23 | 1967-08-29 | Swift & Co | Culturing anaerobic bacteria |
DE2055741A1 (en) * | 1969-11-14 | 1971-05-19 | National Research Development Corp , London | Microbiological growth tray |
EP0006192A1 (en) * | 1978-06-12 | 1980-01-09 | Roche Diagnostics GmbH | Device and procedure for microbiological work |
WO1982002563A1 (en) * | 1981-01-27 | 1982-08-05 | Minnesota Mining & Mfg | Dry culture media |
EP0146143A2 (en) * | 1983-12-20 | 1985-06-26 | Showa Yakuhin Kako Co., Ltd. | Simplified detection implement |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5232838A (en) * | 1991-12-09 | 1993-08-03 | Minnesota Mining And Manufacturing Company | Culture media device and method of use |
US8906645B2 (en) | 2010-12-29 | 2014-12-09 | 3M Innovative Properties Company | Microbial detection article having a water-absorbent filter assembly |
Also Published As
Publication number | Publication date |
---|---|
JPH02167067A (en) | 1990-06-27 |
US5147801A (en) | 1992-09-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA1183094A (en) | Dry culture media | |
USRE35286E (en) | Method and apparatus for culturing with microbiological dry culture medium | |
US5858770A (en) | Cell culture plate with oxygen and carbon dioxide-permeable waterproof sealing membrane | |
US7195912B2 (en) | Hydrogel thin film containing extracellular matrix components | |
JP4183288B2 (en) | Sensor device for detecting microorganisms and method therefor | |
US4801548A (en) | Petri dish for cultivating bacteria and method of inspecting drug susceptibility | |
CA2374020C (en) | Simple culture medium and method for preparation thereof | |
EP0097904A1 (en) | Method and device for rapid diagnosis of dental caries | |
JPS62228147A (en) | Biological indicator for sterilization process | |
JP2002520028A (en) | Vacuum sensing device for detecting microorganisms in blood sample and method thereof | |
CN110168070B (en) | Microbial detection devices comprising adhesive-masked nutrients and methods of using these devices | |
US6770454B2 (en) | Method for detecting microorganisms | |
EP0374905A1 (en) | Culture medium device and method of preparing the same | |
JPH08280377A (en) | Culturing apparatus | |
US6291202B1 (en) | Disc assay device with inoculation pad and methods of use | |
JP3850465B2 (en) | Simple medium and microorganism detection method | |
US5348861A (en) | Device and method for the detection of microorganisms which produce low-molecular-weight metabolites | |
JP3630737B2 (en) | Simple medium | |
JP2553279B2 (en) | Method and device for collecting and enclosing microbes by adhesive tape | |
JPH08336381A (en) | Culture apparatus | |
JP3658023B2 (en) | Equipment and method for detecting environmentally attached bacteria | |
CN2147243Y (en) | Cultivating bottle for testing effect of ultraviolet ray sterilizing | |
CA1039633A (en) | Diagnostic device for liquid samples | |
JPH08256758A (en) | Culturing apparatus | |
JPH08228758A (en) | Culture device |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): DE FR GB |
|
17P | Request for examination filed |
Effective date: 19901219 |
|
D17Q | First examination report despatched (deleted) | ||
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 19940310 |