EA202190867A1 - СПОСОБ ДЕТЕКЦИИ И КОЛИЧЕСТВЕННОГО АНАЛИЗА АЛЛЕРГЕН-СПЕЦИФИЧЕСКИХ АНТИТЕЛ IgE И IgG - Google Patents

СПОСОБ ДЕТЕКЦИИ И КОЛИЧЕСТВЕННОГО АНАЛИЗА АЛЛЕРГЕН-СПЕЦИФИЧЕСКИХ АНТИТЕЛ IgE И IgG

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Publication number
EA202190867A1
EA202190867A1 EA202190867A EA202190867A EA202190867A1 EA 202190867 A1 EA202190867 A1 EA 202190867A1 EA 202190867 A EA202190867 A EA 202190867A EA 202190867 A EA202190867 A EA 202190867A EA 202190867 A1 EA202190867 A1 EA 202190867A1
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EA
Eurasian Patent Office
Prior art keywords
absorbent material
serum sample
blood
quantitative analysis
allergen
Prior art date
Application number
EA202190867A
Other languages
English (en)
Inventor
Рудольф Валента
Виктория Гариб
Феликс Гастагер
Original Assignee
Медицинише Универзитет Вин
Хфд Фертрибс Гез.М.Б.Х.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Медицинише Универзитет Вин, Хфд Фертрибс Гез.М.Б.Х. filed Critical Медицинише Универзитет Вин
Publication of EA202190867A1 publication Critical patent/EA202190867A1/ru

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Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854Immunoglobulins

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Настоящее изобретение относится к способу детекции и/или количественного анализа аллерген-специфических IgE и/или IgG в образце крови или сыворотки млекопитающего, включающему стадии a) переноса образца крови или сыворотки на абсорбирующий материал, b) сушки абсорбирующего материала, содержащего образец крови или сыворотки со стадии а), c) элюирования IgE и/или IgG из высушенного абсорбирующего материала или его части, содержащего высушенный образец крови или сыворотки, посредством нанесения буфера на указанный абсорбирующий материал или его часть, d) выделения элюата со стадии с) и e) нанесения выделенного элюата со стадии d) на тест-систему для одновременной детекции и/или количественного анализа IgE и/или IgG, связывающихся по меньшей мере с двумя аллергенами, где тест-система содержит твердую подложку, содержащую по меньшей мере два аллергена и/или их функциональные фрагменты, связанные с ней, в количестве менее 1 нг каждый.
EA202190867A 2018-10-09 2019-10-08 СПОСОБ ДЕТЕКЦИИ И КОЛИЧЕСТВЕННОГО АНАЛИЗА АЛЛЕРГЕН-СПЕЦИФИЧЕСКИХ АНТИТЕЛ IgE И IgG EA202190867A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT508802018 2018-10-09
PCT/AT2019/060333 WO2020073066A1 (en) 2018-10-09 2019-10-08 METHOD FOR DETECTING AND QUANTIFYING ALLERGEN SPECIFIC IgE AND IgG ANTIBODIES

Publications (1)

Publication Number Publication Date
EA202190867A1 true EA202190867A1 (ru) 2021-07-02

Family

ID=68280634

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202190867A EA202190867A1 (ru) 2018-10-09 2019-10-08 СПОСОБ ДЕТЕКЦИИ И КОЛИЧЕСТВЕННОГО АНАЛИЗА АЛЛЕРГЕН-СПЕЦИФИЧЕСКИХ АНТИТЕЛ IgE И IgG

Country Status (3)

Country Link
EP (1) EP3864416A1 (ru)
EA (1) EA202190867A1 (ru)
WO (1) WO2020073066A1 (ru)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2087348T3 (da) * 2006-12-01 2014-07-07 Statens Seruminstitut Analysemetode under brug af prøveadsorption på filterpapir
US20160327567A1 (en) * 2015-05-07 2016-11-10 Spiriplex, Inc Methods and devices for allergy testing and treatment

Also Published As

Publication number Publication date
EP3864416A1 (en) 2021-08-18
WO2020073066A1 (en) 2020-04-16

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