DK2986701T3 - Polypeptider med proteaseaktivitet og polynukleotider, som koder for dem - Google Patents

Polypeptider med proteaseaktivitet og polynukleotider, som koder for dem Download PDF

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Publication number
DK2986701T3
DK2986701T3 DK14720513.2T DK14720513T DK2986701T3 DK 2986701 T3 DK2986701 T3 DK 2986701T3 DK 14720513 T DK14720513 T DK 14720513T DK 2986701 T3 DK2986701 T3 DK 2986701T3
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DK
Denmark
Prior art keywords
polypeptide
protease
cell
vol
polynucleotide
Prior art date
Application number
DK14720513.2T
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English (en)
Inventor
Martin Simon Borchert
Morten Gjermansen
Peter Rahbek Oestergaard
Lone Baekgaard
Alexander Mauch
Hans Peter Heldt-Hansen
Anne Mette Bhatia Frederiksen
Christina Lunde
Miguel Duarte Guilherme Pereira Toscano
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Novozymes As
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Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK2986701T3 publication Critical patent/DK2986701T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C12C5/004—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C11/00—Fermentation processes for beer
    • C12C11/003—Fermentation of beerwort
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)

Claims (7)

1. Protease valgt fra gruppen bestående af: (a) en protease, som udviser mindst 65% sekvensidentitet med poiypeptidet ifølge SEQ ID NO: 1; og (b) en protease, der kodes for af et polynukieotid, som udviser mindst 65% sekvensidentitet med det modne polypeptids kodningssekvens ifølge SEQ ID NO: 2.
2. Protease ifølge krav 1, som udviser mindst 70%, mindst 75%, mindst 80%, mindst 85%, mindst 90%, mindst 91%, mindst 92%, mindst 93%, mindst 94%, mindst 95%, mindst 96%, mindst 97%, mindst 98%, mindst 99% eller 100% sekvensidentitet med poiypeptidet ifølge SEQ ID NO: 1.
3. Sammensætning, som omfatter proteasen ifølge et hvilket som helst af kravene 1-2.
4. Isoleret polynukieotid, som koder for proteasen ifølge et hvilket som helst af kravene 1-2.
5. Nukleinsyrekonstruktion eller ekspressionsvektor, som omfatter polynukieotidet ifølge krav 4, hvilket polynukieotid er operativt forbundet ti! en eller flere kontrolsekvenser, der styrer fremstillingen af proteasen i en ekspressionsvært.
6. Rekombinant værtscelle, som omfatter polynukieotidet ifølge krav 4, hvilket polynukieotid er operativt forbundet til en eller flere kontrolsekvenser, der styrer fremstillingen af proteasen.
7. Fremgangsmåde til fremstilling af proteasen ifølge et hvilket som helst af kravene 1-2, hvilken fremgangsmåde omfatter dyrkning af en celle, som i sin viidtypeform fremstiller proteasen, under betingelser, der er befordrende for fremstilling af proteasen.
DK14720513.2T 2013-04-18 2014-04-11 Polypeptider med proteaseaktivitet og polynukleotider, som koder for dem DK2986701T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13164263 2013-04-18
PCT/EP2014/057370 WO2014170218A1 (en) 2013-04-18 2014-04-11 Polypeptides having protease activity and polynucleotides encoding same

Publications (1)

Publication Number Publication Date
DK2986701T3 true DK2986701T3 (da) 2019-01-14

Family

ID=48095743

Family Applications (1)

Application Number Title Priority Date Filing Date
DK14720513.2T DK2986701T3 (da) 2013-04-18 2014-04-11 Polypeptider med proteaseaktivitet og polynukleotider, som koder for dem

Country Status (6)

Country Link
US (1) US10131864B2 (da)
EP (1) EP2986701B1 (da)
CN (1) CN105378050A (da)
DK (1) DK2986701T3 (da)
TR (1) TR201819061T4 (da)
WO (1) WO2014170218A1 (da)

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5989870A (en) 1986-04-30 1999-11-23 Rohm Enzyme Finland Oy Method for cloning active promoters
DK564086A (da) 1986-11-25 1988-06-17 Novo Industri As Enzymatisk detergent-additiv
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
CA2058633C (en) 1989-06-13 2000-03-21 Virgil B. Lawlis, Jr. A method for killing cells without lysis
NZ237549A (en) 1990-03-23 1993-06-25 Gist Brocades Nv Production of enhanced levels of enzymes in the seeds of transgenic plants and the use of these seeds
US6395966B1 (en) 1990-08-09 2002-05-28 Dekalb Genetics Corp. Fertile transgenic maize plants containing a gene encoding the pat protein
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
MX9606013A (es) 1994-06-03 1997-12-31 Novo Nordisk Biotech Inc Lacasas purificadas de myceliophthora y acidos nucleicos que codifican para las mismas.
DE69534185T2 (de) 1994-06-30 2006-02-23 Novozymes Biotech, Inc., Davis Nicht-toxisches, nicht-toxigenes, nicht-pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
CN1197965C (zh) 1998-10-26 2005-04-20 诺维信公司 在丝状真菌细胞内构建和筛选目的dna文库
CN1526820B (zh) 1999-03-22 2010-05-26 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
CA2431291C (en) 2000-12-07 2010-09-21 Dsm N.V. Method for the prevention or reduction of haze in beverages
US7151204B2 (en) 2001-01-09 2006-12-19 Monsanto Technology Llc Maize chloroplast aldolase promoter compositions and methods for use thereof
CN105671027A (zh) 2008-09-30 2016-06-15 诺维信股份有限公司 在丝状真菌细胞中使用阳性和阴性选择性基因的方法
RU2531522C2 (ru) * 2009-02-19 2014-10-20 Новозимс А/С Способ пивоварения
MY158663A (en) 2009-02-20 2016-10-31 Danisco Us Inc Fermentation broth formulations
WO2012022745A1 (en) * 2010-08-17 2012-02-23 Novozymes A/S A brewing method

Also Published As

Publication number Publication date
EP2986701A1 (en) 2016-02-24
TR201819061T4 (tr) 2019-01-21
US10131864B2 (en) 2018-11-20
WO2014170218A1 (en) 2014-10-23
CN105378050A (zh) 2016-03-02
US20160053211A1 (en) 2016-02-25
EP2986701B1 (en) 2018-11-14

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