DK2861756T3 - Kinetisk udelukkelsesamplifikation af nukleinsyrebiblioteker - Google Patents
Kinetisk udelukkelsesamplifikation af nukleinsyrebiblioteker Download PDFInfo
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- DK2861756T3 DK2861756T3 DK13731229.4T DK13731229T DK2861756T3 DK 2861756 T3 DK2861756 T3 DK 2861756T3 DK 13731229 T DK13731229 T DK 13731229T DK 2861756 T3 DK2861756 T3 DK 2861756T3
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00585—Parallel processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00585—Parallel processes
- B01J2219/00587—High throughput processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00608—DNA chips
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00614—Delimitation of the attachment areas
- B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00632—Introduction of reactive groups to the surface
- B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00675—In-situ synthesis on the substrate
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/537—Detection characterised by immobilisation to a surface characterised by the capture oligonucleotide acting as a primer
Claims (15)
1. Fremgangsmåde til amplificering af nukleinsyrer, som omfatter (a) tilvejebringelse af (i) et array af amplifikationsteder med et eller flere indfangningsmidler og (ii) et amplifikationsreagens, der omfatter en opløsning, der omfatter et antal af forskellige målnukleinsyrer, hvor antallet af de forskellige målnukleinsyrer i opløsningen overstiger antallet af amplifikationssteder i arrayet, hvor de forskellige målnukleinsyrer har fluidisk adgang til antallet af amplifikationssteder, og hvor hvert af amplifikationsstederne er i stand til at blive besat af adskillige målnukleinsyrer af antallet af forskellige målnukleinsyrer; og (b) reaktion af amplifikationsreagenset med arrayet af amplifikationssteder, således at et antal af amplifikationssteder hvert omfatter klonale amplikoner fra en individuel målnukleinsyre fra opløsningen, hvor reaktionen omfatter samtidigy (i) transport af de forskellige målnukleinsyrer til amplifikationsstederne til podning i amplifikationsstederne og (ii) amplificering af målnukleinsyrerne, der er podet i amplifikationsstederne, hvor amplifikationshastigheden overstiger podningshastigheden, således at de dannede amplikoner forhindrer, at yderligere målnukleinsyrer podes i hvert af antallet af amplifikationssteder, og derved forhindrer, at de amplificeres i hvert af antallet af amplifikationssteder.
2. Fremgangsmåde ifølge krav 1, hvor hvert af amplifikationsstederne omfatter et antal af indfangningsmidler, der er i stand til at binde til de forskellige målnukleinsyrer i opløsningen.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor arrayet af amplifikationssteder omfatter et array af træk på en overflade, og eventuelt hvor arealet for hvert af trækkene er større end diameteren af det udelukkede volumen af målnukleinsyrerne, der transporteres til amplifikationsstederne.
4. Fremgangsmåde ifølge krav 3, hvor trækkene er ikke-fortløbende og er adskilt af mellemrumsområder af overfladen, der mangler indfangningsmidlerne.
5. Fremgangsmåde ifølge krav 3 eller 4, hvor hvert af trækkene omfatter et korn, en brønd, en kanal, en kam, et fremspring eller en kombination deraf.
6. Fremgangsmåde ifølge krav 1 eller 2, hvor arrayet af amplifikationssteder omfatter korn i opløsning eller korn på en overflade.
7. Fremgangsmåde ifølge krav 2, hvor indfangningsmidlerne omfatter indfangningsnukleinsyrer, der er komplementære til de forskellige målnukleinsyrer, og eventuelt hvor de forskellige målnukleinsyrer omfatter universelle sekvenser, der er komplementære til indfangningsnukleinsyrerne.
8. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor hvert af amplifikationsstederne omfatter et antal primere, der anvendes til frembringelse af amplikonerne i (b).
9. Fremgangsmåde ifølge krav 8, hvor arrayet af amplifikationssteder omfatter et array af træk på en overflade, hvor trækkene er ikke-fortløbende og er adskilt af mellemrumsområder af overfladen, der mangler primerne, der anvendes til frembringelse af amplikonerne i (b).
10. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor amplifikationsreagenset yderligere omfatter rekombinase og et enkeltstrenget bindingsprotein, og eventuelt hvor amplifikationsreagenset yderligere omfatter et middel til molekylær sammenklumpning.
11. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor amplificeringen af målnukleinsyrerne, der transporteres til amplifikationsstederne, ikke indbefatter en denatureringscyklus.
12. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor antallet af amplifikationssteder, der omfatter en klonal population af amplikoner, overstiger 40 % af amplifikationsstederne, som de forskellige målnukleinsyrer har fluidisk adgang til.
13. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor de forskellige målnukleinsyrer transporteres aktivt til amplifikationsstederne assisteret af påføring af et elektrisk felt.
14. Fremgangsmåde ifølge krav 13, hvor det elektriske felt øges, efterhånden som reaktionen forløber over tid.
15. Fremgangsmåde ifølge krav 13 hvor arrayet af amplifikationssteder omfatter et array af ikke-fortløbende træk på en overflade, hvilke træk er adskilt af mellemrumsområder af overfladen, eventuelt hvor arealet for hvert af trækkene er større end diameteren af det udelukkede volumen af målnukleinsyrerne, der transporteres til amplifikationsstederne, eventuelt hvor de forskellige målnukleinsyrer aktivt frastødes fra mellemrumsområder ved forstærkning af et andet elektrisk felt.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
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US201261660487P | 2012-06-15 | 2012-06-15 | |
US201261715478P | 2012-10-18 | 2012-10-18 | |
US13/783,043 US8895249B2 (en) | 2012-06-15 | 2013-03-01 | Kinetic exclusion amplification of nucleic acid libraries |
PCT/US2013/045491 WO2013188582A1 (en) | 2012-06-15 | 2013-06-12 | Kinetic exclusion amplification of nucleic acid libraries |
Publications (1)
Publication Number | Publication Date |
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DK2861756T3 true DK2861756T3 (da) | 2018-04-09 |
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Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK18150783.1T DK3366781T3 (da) | 2012-06-15 | 2013-06-12 | Amplifikation ved kinetisk udelukkelse af nukleinsyrebiblioteker |
DK13731229.4T DK2861756T3 (da) | 2012-06-15 | 2013-06-12 | Kinetisk udelukkelsesamplifikation af nukleinsyrebiblioteker |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK18150783.1T DK3366781T3 (da) | 2012-06-15 | 2013-06-12 | Amplifikation ved kinetisk udelukkelse af nukleinsyrebiblioteker |
Country Status (12)
Country | Link |
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US (6) | US8895249B2 (da) |
EP (2) | EP2861756B1 (da) |
JP (3) | JP6014249B2 (da) |
KR (2) | KR101954343B1 (da) |
CN (2) | CN104379766B (da) |
AU (3) | AU2013274283B2 (da) |
CA (1) | CA2867716C (da) |
DK (2) | DK3366781T3 (da) |
ES (2) | ES2662098T3 (da) |
HK (2) | HK1204012A1 (da) |
IL (2) | IL234865B (da) |
WO (1) | WO2013188582A1 (da) |
Families Citing this family (132)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8383345B2 (en) | 2008-09-12 | 2013-02-26 | University Of Washington | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
US20120156728A1 (en) | 2010-12-17 | 2012-06-21 | Life Technologies Corporation | Clonal amplification of nucleic acid on solid surface with template walking |
US9309566B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
PL2556171T3 (pl) | 2010-04-05 | 2016-04-29 | Prognosys Biosciences Inc | Oznaczenia biologiczne kodowane przestrzennie |
AU2011312218B2 (en) | 2010-10-04 | 2015-11-05 | Sequencing Health, Inc. | Systems and methods for automated reusable parallel biological reactions |
US9399217B2 (en) | 2010-10-04 | 2016-07-26 | Genapsys, Inc. | Chamber free nanoreactor system |
US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
JP6017458B2 (ja) | 2011-02-02 | 2016-11-02 | ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション | 大量並列連続性マッピング |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
US9926596B2 (en) | 2011-05-27 | 2018-03-27 | Genapsys, Inc. | Systems and methods for genetic and biological analysis |
EP2771103B1 (en) | 2011-10-28 | 2017-08-16 | Illumina, Inc. | Microarray fabrication system and method |
CN106591103B (zh) | 2011-12-01 | 2021-06-04 | 吉纳普赛斯股份有限公司 | 用于高效电子测序与检测的系统和方法 |
US9012022B2 (en) | 2012-06-08 | 2015-04-21 | Illumina, Inc. | Polymer coatings |
US8895249B2 (en) * | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
US9512422B2 (en) | 2013-02-26 | 2016-12-06 | Illumina, Inc. | Gel patterned surfaces |
EP3919617A1 (en) | 2013-03-13 | 2021-12-08 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
DK2969479T3 (da) | 2013-03-13 | 2021-08-02 | Illumina Inc | Væskeanordninger i flere lag og fremgangsmåder til deres fremstilling |
US9193998B2 (en) | 2013-03-15 | 2015-11-24 | Illumina, Inc. | Super resolution imaging |
US20140274747A1 (en) | 2013-03-15 | 2014-09-18 | Illumina, Inc. | Super resolution imaging |
CN105051214B (zh) | 2013-03-15 | 2018-12-28 | 吉纳普赛斯股份有限公司 | 用于生物分析的系统和方法 |
WO2014210225A1 (en) | 2013-06-25 | 2014-12-31 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
EP3038834B1 (en) | 2013-08-30 | 2018-12-12 | Illumina, Inc. | Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces |
US10540783B2 (en) | 2013-11-01 | 2020-01-21 | Illumina, Inc. | Image analysis useful for patterned objects |
EP3080300B1 (en) | 2013-12-11 | 2020-09-02 | Genapsys Inc. | Systems and methods for biological analysis and computation |
CN105874385B (zh) * | 2013-12-19 | 2021-08-20 | Illumina公司 | 包括纳米图案化表面的基底及其制备方法 |
EP3957750A1 (en) | 2013-12-20 | 2022-02-23 | Illumina, Inc. | Preserving genomic connectivity information in fragmented genomic dna samples |
US9677132B2 (en) | 2014-01-16 | 2017-06-13 | Illumina, Inc. | Polynucleotide modification on solid support |
EP3556864B1 (en) | 2014-04-18 | 2020-12-09 | Genapsys, Inc. | Methods and systems for nucleic acid amplification |
US10443087B2 (en) | 2014-06-13 | 2019-10-15 | Illumina Cambridge Limited | Methods and compositions for preparing sequencing libraries |
GB201416422D0 (en) * | 2014-09-17 | 2014-10-29 | Illumina Cambridge Ltd | Flexible tape-based chemistry apparatus |
US9982244B2 (en) * | 2014-09-29 | 2018-05-29 | Illumina Cambridge Limited | Recombinase mutants |
RU2709655C2 (ru) | 2014-10-17 | 2019-12-19 | Иллумина Кембридж Лимитед | Транспозиция с сохранением сцепления генов |
KR102590448B1 (ko) | 2014-11-11 | 2023-10-17 | 일루미나 케임브리지 리미티드 | 핵산의 모노클로날 클러스터를 형성시키고 시퀀싱하기 위한 방법 및 어레이 |
CN107223161B (zh) * | 2014-12-15 | 2022-03-22 | 亿明达股份有限公司 | 用于基底上的单分子放置的组合物和方法 |
EP3901282B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
CN108449971B (zh) | 2015-04-14 | 2020-10-16 | 亿明达股份有限公司 | 用于改进对光发射的检测的结构化基底及涉及其的方法 |
CN113624953A (zh) | 2015-05-11 | 2021-11-09 | 亿明达股份有限公司 | 用于发现和分析治疗剂的平台 |
EP3303614B1 (en) | 2015-05-29 | 2020-03-11 | Illumina Cambridge Limited | Enhanced utilization of surface primers in clusters |
CN107849600A (zh) | 2015-06-09 | 2018-03-27 | 生命技术公司 | 用于分子标记的方法、系统、组合物、试剂盒、装置和计算机可读媒体 |
EP3763818A1 (en) | 2015-10-06 | 2021-01-13 | Pierce Biotechnology, Inc. | Devices and methods for producing proteins |
AU2017207259B2 (en) | 2016-01-11 | 2019-06-27 | Illumina Singapore Pte Ltd | Detection apparatus having a microfluorometer, a fluidic system, and a flow cell latch clamp module |
AU2017241670B2 (en) | 2016-03-28 | 2020-10-08 | Illumina, Inc. | Recombinase mutants |
EP3377226B1 (en) | 2016-03-28 | 2021-02-17 | Illumina, Inc. | Multi-plane microarrays |
ES2786974T3 (es) | 2016-04-07 | 2020-10-14 | Illumina Inc | Métodos y sistemas para la construcción de bibliotecas de ácidos nucleicos normalizadas |
US10544456B2 (en) | 2016-07-20 | 2020-01-28 | Genapsys, Inc. | Systems and methods for nucleic acid sequencing |
KR20230003255A (ko) * | 2016-07-22 | 2023-01-05 | 오레곤 헬스 앤드 사이언스 유니버시티 | 단일 세포 전체 게놈 라이브러리 및 이의 제조를 위한 조합 인덱싱 방법 |
GB201704754D0 (en) * | 2017-01-05 | 2017-05-10 | Illumina Inc | Kinetic exclusion amplification of nucleic acid libraries |
GB201701689D0 (en) | 2017-02-01 | 2017-03-15 | Illumia Inc | System and method with fiducials of non-closed shapes |
GB201701688D0 (en) | 2017-02-01 | 2017-03-15 | Illumia Inc | System and method with fiducials in non-recliner layouts |
GB201701686D0 (en) | 2017-02-01 | 2017-03-15 | Illunina Inc | System & method with fiducials having offset layouts |
WO2018185871A1 (ja) | 2017-04-05 | 2018-10-11 | 株式会社日立ハイテクノロジーズ | 核酸増幅方法および核酸解析用装置 |
EP3615691B1 (en) | 2017-04-23 | 2021-05-26 | Illumina, Inc. | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
EP3615671B1 (en) | 2017-04-23 | 2021-07-21 | Illumina Cambridge Limited | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
CA3060979C (en) | 2017-04-23 | 2023-07-11 | Illumina Cambridge Limited | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
CN110785497A (zh) | 2017-04-23 | 2020-02-11 | 伊鲁米纳剑桥有限公司 | 用于改进编索引的核酸文库中的样品鉴定的组合物和方法 |
CN107254572B (zh) * | 2017-06-01 | 2019-07-02 | 首钢集团有限公司 | 一种冷轧硅锰双相钢表面麻点缺陷的控制方法 |
SG10202108175RA (en) | 2017-06-07 | 2021-09-29 | Univ Oregon Health & Science | Single cell whole genome libraries for methylation sequencing |
EP3662482A1 (en) | 2017-07-31 | 2020-06-10 | Illumina Inc. | Sequencing system with multiplexed biological sample aggregation |
EP3662083A4 (en) | 2017-08-01 | 2021-03-03 | Illumina, Inc. | SPATIAL INDEXING OF GENETIC MATERIAL AND LIBRARY MANUFACTURING USING HYDROGEL BEADS AND FLOW CELLS |
JP7169345B2 (ja) | 2017-09-01 | 2022-11-10 | 深▲セン▼華大智造科技股▲ふん▼有限公司 | シリコンセンサと一体型の注入形成型のマイクロ流体/流体カートリッジ |
SG11202002516WA (en) | 2017-09-21 | 2020-04-29 | Genapsys Inc | Systems and methods for nucleic acid sequencing |
CN107622185B (zh) * | 2017-10-27 | 2020-08-21 | 领航基因科技(杭州)有限公司 | 一种数字pcr浓度计算方法 |
ES2937927T3 (es) * | 2018-01-29 | 2023-04-03 | St Jude Childrens Res Hospital Inc | Método para la amplificación de ácidos nucleicos |
US20190367909A1 (en) | 2018-04-02 | 2019-12-05 | Illumina, Inc. | Compositions and methods for making controls for sequence-based genetic testing |
EP3810774B1 (en) | 2018-06-04 | 2023-09-13 | Illumina, Inc. | Methods of making high-throughput single-cell transcriptome libraries |
CN113056327A (zh) | 2018-07-23 | 2021-06-29 | Dna斯克瑞普特公司 | 核酸链的大规模平行酶促合成 |
US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
SG11202101944RA (en) | 2018-08-28 | 2021-03-30 | 10X Genomics Inc | Increasing spatial array resolution |
AU2019391274A1 (en) | 2018-12-05 | 2021-01-07 | Illumina Cambridge Limited | Methods and compositions for cluster generation by bridge amplification |
KR20210104554A (ko) | 2018-12-18 | 2021-08-25 | 일루미나 케임브리지 리미티드 | 단일 표면 프라이머를 사용하는 페어드 엔드 서열분석을 위한 방법 및 조성물 |
WO2020132103A1 (en) * | 2018-12-19 | 2020-06-25 | Illumina, Inc. | Methods for improving polynucleotide cluster clonality priority |
US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
AU2020232618A1 (en) | 2019-03-01 | 2021-04-08 | Illumina, Inc. | High-throughput single-nuclei and single-cell libraries and methods of making and of using |
GB201905303D0 (en) | 2019-04-15 | 2019-05-29 | Thermo Fisher Scient Geneart Gmbh | Multiplex assembly of nucleic acid molecules |
CN110694599A (zh) * | 2019-09-26 | 2020-01-17 | 浙江海洋大学 | 一种聚丙烯亚胺修饰的磁性纳晶纤维素分子印迹聚合物的制备方法 |
WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
EP3927824A2 (en) | 2019-12-19 | 2021-12-29 | Illumina, Inc. | High-throughput single-cell libraries and methods of making and of using |
AU2020412766A1 (en) | 2019-12-23 | 2022-06-09 | 10X Genomics, Inc. | Methods for spatial analysis using RNA-templated ligation |
US11053540B1 (en) | 2020-01-17 | 2021-07-06 | Element Biosciences, Inc. | High performance fluorescence imaging module for genomic testing assay |
EP4092135A4 (en) * | 2020-01-17 | 2023-01-18 | MGI Tech Co., Ltd. | METHODS FOR SYNCHRONOUS SEQUENCING OF SENSE STRAND AND ANTISENSE STRAND OF DNA |
US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
US11821035B1 (en) | 2020-01-29 | 2023-11-21 | 10X Genomics, Inc. | Compositions and methods of making gene expression libraries |
US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
FI3969618T3 (fi) | 2020-03-09 | 2023-10-18 | Illumina Inc | Menetelmiä polynukleotidien sekvensointiin |
US20220251626A1 (en) | 2020-03-09 | 2022-08-11 | Illumina, Inc. | Improvements in nucleic acid sequencing |
MX2022012184A (es) | 2020-03-30 | 2022-10-27 | Illumina Inc | Metodos y composiciones para preparar genotecas de acido nucleico. |
CN115916999A (zh) | 2020-04-22 | 2023-04-04 | 10X基因组学有限公司 | 用于使用靶向rna耗竭进行空间分析的方法 |
US11188778B1 (en) | 2020-05-05 | 2021-11-30 | Illumina, Inc. | Equalization-based image processing and spatial crosstalk attenuator |
EP4150065A2 (en) | 2020-05-12 | 2023-03-22 | Illumina Inc | Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase |
WO2021237087A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
EP4153775A1 (en) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
EP4162074A1 (en) | 2020-06-08 | 2023-04-12 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
WO2021252617A1 (en) | 2020-06-09 | 2021-12-16 | Illumina, Inc. | Methods for increasing yield of sequencing libraries |
WO2021252591A1 (en) | 2020-06-10 | 2021-12-16 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
WO2021263111A1 (en) | 2020-06-25 | 2021-12-30 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
JP2023532231A (ja) | 2020-06-30 | 2023-07-27 | イルミナ インコーポレイテッド | 傷なしdnaを生成するための触媒的に制御された合成による配列決定 |
US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
CN116018412A (zh) | 2020-07-08 | 2023-04-25 | Illumina公司 | 作为转座体载体的小珠 |
US11913067B2 (en) | 2020-09-14 | 2024-02-27 | Illumina, Inc. | Compositions and methods for amplifying polynucleotides |
US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
WO2022140028A1 (en) | 2020-12-21 | 2022-06-30 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
KR20230154303A (ko) | 2021-01-29 | 2023-11-07 | 일루미나, 인코포레이티드 | 폴리뉴클레오티드로 플로우 셀의 시딩 효율을 개선하기위한 방법, 조성물 및 키트 |
CN117222747A (zh) | 2021-03-22 | 2023-12-12 | 伊鲁米纳剑桥有限公司 | 用于改善核酸簇克隆性的方法 |
WO2022272260A1 (en) * | 2021-06-23 | 2022-12-29 | Illumina, Inc. | Compositions, methods, kits, cartridges, and systems for sequencing reagents |
US20220414853A1 (en) | 2021-06-25 | 2022-12-29 | Illumina, Inc. | Fiducials for use in registration of a patterned surface |
KR20240025515A (ko) | 2021-06-25 | 2024-02-27 | 일루미나, 인코포레이티드 | 선형 푸리에 기점 |
US11455487B1 (en) | 2021-10-26 | 2022-09-27 | Illumina Software, Inc. | Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling |
CA3226451A1 (en) | 2021-07-21 | 2023-01-26 | Sam Reed | Method and system comprising a cartridge for sequencing target polynucleotides |
GB202110479D0 (en) | 2021-07-21 | 2021-09-01 | Dnae Diagnostics Ltd | Compositions, kits and methods for sequencing target polynucleotides |
GB202110485D0 (en) | 2021-07-21 | 2021-09-01 | Dnae Diagnostics Ltd | Compositions, kits and methods for sequencing target polynucleotides |
WO2023004357A1 (en) * | 2021-07-23 | 2023-01-26 | Illumina, Inc. | Methods for preparing substrate surface for dna sequencing |
WO2023034489A1 (en) | 2021-09-01 | 2023-03-09 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
WO2023052629A2 (en) | 2021-09-30 | 2023-04-06 | Illumina Cambridge Limited | Blocker methods |
CA3223595A1 (en) | 2021-12-16 | 2023-06-22 | Xiaoyu Ma | Hybrid clustering |
US20230212667A1 (en) | 2021-12-29 | 2023-07-06 | Illumina Cambridge Limited | Methods of nucleic acid sequencing using surface-bound primers |
WO2023175021A1 (en) | 2022-03-15 | 2023-09-21 | Illumina, Inc. | Methods of preparing loop fork libraries |
CA3223653A1 (en) | 2022-03-15 | 2023-09-21 | Aathavan KARUNAKARAN | Concurrent sequencing of forward and reverse complement strands on separate polynucleotides for methylation detection |
AU2023244497A1 (en) | 2022-03-31 | 2024-01-18 | Illumina, Inc. | Paired-end re-synthesis using blocked p5 primers |
CA3223722A1 (en) | 2022-04-07 | 2023-10-12 | Illumina, Inc. | Altered cytidine deaminases and methods of use |
WO2023209606A1 (en) | 2022-04-29 | 2023-11-02 | Illumina Cambridge Limited | Methods and systems for encapsulating lyophilised microspheres |
US20240033738A1 (en) | 2022-07-27 | 2024-02-01 | Illumina, Inc. | Flow cell based motion system calibration and control methods |
Family Cites Families (89)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US169513A (en) | 1875-11-02 | Improvement in electric telegraphs | ||
US4787963A (en) | 1987-05-04 | 1988-11-29 | Syntro Corporation | Method and means for annealing complementary nucleic acid molecules at an accelerated rate |
GB8810400D0 (en) | 1988-05-03 | 1988-06-08 | Southern E | Analysing polynucleotide sequences |
US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
WO1990002327A1 (en) | 1988-08-18 | 1990-03-08 | AUSTRALIAN MEMBRANE AND BIOTECHNOLOGY RESEARCH INSTITUTE LTD., Commonwealth Scientific and Industrial Research Organization | Improvements in sensitivity and selectivity of ion channel membrane biosensors |
CA2044616A1 (en) | 1989-10-26 | 1991-04-27 | Roger Y. Tsien | Dna sequencing |
JPH05507025A (ja) | 1990-04-11 | 1993-10-14 | ルドヴィグ・インスティテュート・フォー・キャンサー・リサーチ | 逐次的化学反応が可能な方法および装置 |
US5223414A (en) | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
US5227265A (en) | 1990-11-30 | 1993-07-13 | Eastman Kodak Company | Migration imaging system |
US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
US5605662A (en) | 1993-11-01 | 1997-02-25 | Nanogen, Inc. | Active programmable electronic devices for molecular biological analysis and diagnostics |
US6051380A (en) | 1993-11-01 | 2000-04-18 | Nanogen, Inc. | Methods and procedures for molecular biological analysis and diagnostics |
KR100230718B1 (ko) | 1994-03-16 | 1999-11-15 | 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 | 등온 가닥 변위 핵산 증폭법 |
US5552278A (en) | 1994-04-04 | 1996-09-03 | Spectragen, Inc. | DNA sequencing by stepwise ligation and cleavage |
US5641658A (en) | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
US5728532A (en) * | 1996-05-31 | 1998-03-17 | Ackley; Donald E. | Electrode configuration for matrix addressing of a molecular detection device |
GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
US6023540A (en) | 1997-03-14 | 2000-02-08 | Trustees Of Tufts College | Fiber optic sensor with encoded microspheres |
US6327410B1 (en) | 1997-03-14 | 2001-12-04 | The Trustees Of Tufts College | Target analyte sensors utilizing Microspheres |
ATE269908T1 (de) | 1997-04-01 | 2004-07-15 | Manteia S A | Methode zur sequenzierung von nukleinsäuren |
US6451616B1 (en) | 1997-05-19 | 2002-09-17 | Robert W. Odom | Analysis of molecules bound to solid surfaces using selective bond cleavage processes |
US6511803B1 (en) | 1997-10-10 | 2003-01-28 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
WO2000046408A1 (en) | 1999-02-04 | 2000-08-10 | Sloan-Kettering Institute For Cancer Research | Process for dna replication |
US6699693B1 (en) | 1999-02-04 | 2004-03-02 | Sloan-Kettering Institute For Cancer Research | Process for DNA replication |
US20050181440A1 (en) | 1999-04-20 | 2005-08-18 | Illumina, Inc. | Nucleic acid sequencing using microsphere arrays |
US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
US6355431B1 (en) | 1999-04-20 | 2002-03-12 | Illumina, Inc. | Detection of nucleic acid amplification reactions using bead arrays |
DK1923471T3 (da) | 1999-04-20 | 2013-04-02 | Illumina Inc | Detektion af nukleinsyrereaktioner på bead-arrays |
US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
CA2399733C (en) | 2000-02-07 | 2011-09-20 | Illumina, Inc. | Nucleic acid detection methods using universal priming |
US6913884B2 (en) | 2001-08-16 | 2005-07-05 | Illumina, Inc. | Compositions and methods for repetitive use of genomic DNA |
US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
US6770441B2 (en) | 2000-02-10 | 2004-08-03 | Illumina, Inc. | Array compositions and methods of making same |
AU8288101A (en) | 2000-07-07 | 2002-01-21 | Visigen Biotechnologies Inc | Real-time sequence determination |
EP1354064A2 (en) | 2000-12-01 | 2003-10-22 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
AR031640A1 (es) | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
ES2305289T3 (es) | 2001-04-20 | 2008-11-01 | The Penn State Research Foundation | Metodo de manipulacion de acidos nucleicos. |
GB0127564D0 (en) | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
US20040002090A1 (en) | 2002-03-05 | 2004-01-01 | Pascal Mayer | Methods for detecting genome-wide sequence variations associated with a phenotype |
ATE552349T1 (de) * | 2002-05-10 | 2012-04-15 | Hope City | Pyrophosphorolyse-aktivierte polymerisation (pap) |
AU2003259350A1 (en) | 2002-08-23 | 2004-03-11 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
US7595883B1 (en) | 2002-09-16 | 2009-09-29 | The Board Of Trustees Of The Leland Stanford Junior University | Biological analysis arrangement and approach therefor |
CA2498764C (en) | 2002-09-20 | 2015-11-10 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
ES2380893T3 (es) | 2003-01-29 | 2012-05-21 | 454 Life Sciences Corporation | Amplificación de ácidos nucleicos en emulsión en perlas |
CA2528577C (en) | 2003-06-20 | 2012-08-07 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
EP2918595B1 (en) | 2003-07-05 | 2019-12-11 | The Johns-Hopkins University | Method and compositions for detection and enumeration of genetic variations |
EP3673986A1 (en) | 2004-01-07 | 2020-07-01 | Illumina Cambridge Limited | Improvements in or relating to molecular arrays |
EP1790202A4 (en) | 2004-09-17 | 2013-02-20 | Pacific Biosciences California | APPARATUS AND METHOD FOR ANALYZING MOLECULES |
DK2463386T3 (da) | 2005-06-15 | 2017-07-31 | Complete Genomics Inc | Nukleinsyreanalyse ved hjælp af tilfældige blandinger af ikke-overlappende fragmenter |
ES2420831T3 (es) | 2005-07-25 | 2013-08-27 | Alere San Diego, Inc. | Procedimientos para multiplexar la ampliación de la recombinasa polimerasa |
US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
US8043837B2 (en) * | 2006-03-07 | 2011-10-25 | Cargill, Incorporated | Aldolases, nucleic acids encoding them and methods for making and using them |
US20080009420A1 (en) | 2006-03-17 | 2008-01-10 | Schroth Gary P | Isothermal methods for creating clonal single molecule arrays |
EP2018622B1 (en) | 2006-03-31 | 2018-04-25 | Illumina, Inc. | Systems for sequence by synthesis analysis |
US7702468B2 (en) | 2006-05-03 | 2010-04-20 | Population Diagnostics, Inc. | Evaluating genetic disorders |
AU2007298650B2 (en) | 2006-05-04 | 2013-10-17 | Abbott Diagnostics Scarborough, Inc. | Recombinase polymerase amplification |
CA2666517A1 (en) | 2006-10-23 | 2008-05-02 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
US20080108804A1 (en) | 2006-11-02 | 2008-05-08 | Kabushiki Kaisha Dnaform | Method for modifying RNAS and preparing DNAS from RNAS |
GB2457851B (en) | 2006-12-14 | 2011-01-05 | Ion Torrent Systems Inc | Methods and apparatus for measuring analytes using large scale fet arrays |
US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
EP2121983A2 (en) | 2007-02-02 | 2009-11-25 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
AU2008276308A1 (en) | 2007-07-13 | 2009-01-22 | The Board Of Trustees Of The Leland Stanford Junior University | Method and apparatus using electric field for improved biological assays |
AU2009251881B2 (en) | 2008-03-31 | 2015-03-26 | Pacific Biosciences Of California, Inc | Single molecule loading methods and compositions |
WO2010037085A1 (en) | 2008-09-29 | 2010-04-01 | The Board Of Trustees Of The University Of Illinois | Dna sequencing and amplification systems using nanoscale field effect sensor arrays |
US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
EP2379748A4 (en) | 2008-12-23 | 2012-08-29 | Illumina Inc | MULTIBASE RELEASE FOR LONG READINGS IN SEQUENCING BY SYNTHESIS PROTOCOLS |
US9334531B2 (en) * | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
JP5815518B2 (ja) * | 2009-07-17 | 2015-11-17 | キヤノン ユー.エス. ライフ サイエンシズ, インコーポレイテッドCanon U.S. Life Sciences, Inc. | マイクロ流体装置においてdnaを単離する方法及びシステム |
WO2011038241A1 (en) | 2009-09-25 | 2011-03-31 | President And Fellows Of Harvard College | Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides |
EP2539464B1 (en) | 2010-02-23 | 2016-11-16 | Illumina, Inc. | Amplification methods to minimise sequence specific bias |
WO2011159942A1 (en) | 2010-06-18 | 2011-12-22 | Illumina, Inc. | Conformational probes and methods for sequencing nucleic acids |
US20120083417A1 (en) * | 2010-09-23 | 2012-04-05 | Centrillion Technology Holding Corporation | Native-extension parallel sequencing |
AU2011312218B2 (en) | 2010-10-04 | 2015-11-05 | Sequencing Health, Inc. | Systems and methods for automated reusable parallel biological reactions |
US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
US20130344540A1 (en) * | 2011-02-03 | 2013-12-26 | Mark T. Reed | Methods for minimizing sequence specific bias |
CN102212621B (zh) * | 2011-05-09 | 2014-01-08 | 中国人民解放军第四军医大学 | 一种用于乙型肝炎病毒基因分型的检测试剂盒及检测方法 |
EP2718465B1 (en) * | 2011-06-09 | 2022-04-13 | Illumina, Inc. | Method of making an analyte array |
EP2771103B1 (en) | 2011-10-28 | 2017-08-16 | Illumina, Inc. | Microarray fabrication system and method |
US9012022B2 (en) | 2012-06-08 | 2015-04-21 | Illumina, Inc. | Polymer coatings |
US8895249B2 (en) * | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
-
2013
- 2013-03-01 US US13/783,043 patent/US8895249B2/en active Active
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