DK2611290T3 - Fremgangsmåde til kryopræservering af humane sperma-tozoer uden sædplasma ved anvendelse af en hurtig og enkel aseptisk vitrifikation-devitrifikationsproces - Google Patents
Fremgangsmåde til kryopræservering af humane sperma-tozoer uden sædplasma ved anvendelse af en hurtig og enkel aseptisk vitrifikation-devitrifikationsproces Download PDFInfo
- Publication number
- DK2611290T3 DK2611290T3 DK11713518.6T DK11713518T DK2611290T3 DK 2611290 T3 DK2611290 T3 DK 2611290T3 DK 11713518 T DK11713518 T DK 11713518T DK 2611290 T3 DK2611290 T3 DK 2611290T3
- Authority
- DK
- Denmark
- Prior art keywords
- spermatozoa
- vitrification
- medium
- devitrification
- straw
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Mechanical Engineering (AREA)
- Hematology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Claims (5)
- PATENTKRAY1. Fremgangsmåde til kryopræservering af humane spermatozoer uden sædplasma ved anvendelse af en hurtig og enkel aseptisk vitrifikation-devitrifikationsproces, nævnte fremgangsmåde omfattende: (a) tilvejebringelse af spermatozoer uden sædvæske; (b) resuspendering og blanding af spermatozoerne opnået i trin (a) i et 1:1 volumetrisk forhold med et vitrifikationsmedium omfattende: i) buffermedium til spermatozoer, ii) et ikke-permeabelt kryobeskyttelsesmiddel med en slutkoncentrati-on på 0,15 til 0,30 M, og iii) et serum suppleret med Dextran; (c) blanding og justering af slutkoncentrationen af spermatozoer i et område fra 0,2 x 106 til 1,8 x 106 spermatozoer pr. 100 pi af nævnte vitrifikationsmedium; (d) anbringelse af 100 pi af blandingen af spermatozoer og vitrifikationsmedium umiddelbart efter trin (c) i et 0,25 ml strå, efterladende 1 cm uden væske i begge ender, dette første strå placeres indeni i et andet 0,5 mi strå der forsegles i begge ender, det første og andet strå placeres vandret i en termiskledende beholder; (e) nedsænkning af 0,5 ml strået fra trin d) med sædindholdet i vandret position i flydende N2; (i) opbevaring af sædprøven indeni stråene ved en temperatur mellem - 75°C og -85°C; (g) devitrifikation af prøven ved indføring af strået indeholdende sædprøven i et devitrifikationsmedium omfattende: i) buffermedium til spermatozoer, ii) Humant tubalfiuid suppleret med 1% vægt / volumen serumaibumin fra kvæg, og iii) et serum suppleret med Dextran; hvor devitrifikation udføres først ved placering af prøverne der skal devitrifike-res i en beholder med flydende N2 og derefter indføring af tre 0,25 ml strå med prøve i et rør med 5 ml devitrifikationsmedium inkuberet ved en temperatur i området fra 36,5 til 37,5°C i en varmeblok.
- 2. Fremgangsmåde til kryo præserve ring af spermatozoer ifølge krav 1, hvor den ikke-permeabie kryobeskyttelsesmiddel af trin (b) er sakkarose.
- 3. Fremgangsmåde til kryopræservering af spermatozoer ifølge krav 1, hvor slutmængden af spermatozoer i trin (c) justeres i et område fra 0,5 x 1Q6 til 1,8 x 106.
- 4. Fremgangsmåde til kryopræservering af spermatozoer ifølge krav 1, hvor det termiskledende materiale i beholderen under vitrifikationstrinnel i trin (d) er plastik.
- 5. Fremgangsmåde til kryopræservering af spermatozoer ifølge krav 1, hvor nævnte nedsænkning i flydende N2 i trin (e) er gjort i løbet af 5 sekunder.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CL2010000920A CL2010000920A1 (es) | 2010-08-30 | 2010-08-30 | Metodo para criopreservar espermatozoides humanos libres de plasma seminal, mediante un proceso rapido y simple de vitrificacion - desvitrificacion aseptica; un kit portable para el montaje del metodo. |
| PCT/IB2011/050651 WO2012028967A2 (en) | 2010-08-30 | 2011-02-16 | Method for cryopreservation of human spermatozoa free from seminal plasma using a fast and simple aseptic vitrification-devitrification process; portable kit for carrying out the method; and use of the same for treatment of disorders related to reproductive failures |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2611290T3 true DK2611290T3 (da) | 2017-07-31 |
Family
ID=43971246
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK11713518.6T DK2611290T3 (da) | 2010-08-30 | 2011-02-16 | Fremgangsmåde til kryopræservering af humane sperma-tozoer uden sædplasma ved anvendelse af en hurtig og enkel aseptisk vitrifikation-devitrifikationsproces |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US9005886B2 (da) |
| EP (1) | EP2611290B1 (da) |
| AU (1) | AU2011298099A1 (da) |
| CA (1) | CA2809571A1 (da) |
| CL (1) | CL2010000920A1 (da) |
| DK (1) | DK2611290T3 (da) |
| ES (1) | ES2633123T3 (da) |
| WO (1) | WO2012028967A2 (da) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3152291B1 (en) | 2014-06-09 | 2021-07-21 | Somnio Global Holdings, LLC | Capillary assisted vitrification processes and devices |
| MX2017000231A (es) * | 2014-07-09 | 2017-06-27 | Genentech Inc | Ajuste del ph para mejorar la recuperacion por descongelamiento de bancos de celulas. |
| WO2017099865A1 (en) | 2015-12-07 | 2017-06-15 | Coopersurgical, Inc. | Low temperature specimen carriers and related methods |
| US20210186007A1 (en) | 2018-05-25 | 2021-06-24 | Universidad De La Frontera | Method for the vitrification of human semen and portable kit for the application of said method |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE62578T1 (de) * | 1984-11-09 | 1991-05-15 | Cryologic Pty Ltd | Kryogensystem fuer biologisches material. |
| US20060046243A1 (en) | 2004-08-27 | 2006-03-02 | Tyho-Galileo Research Laboratories | Method for vitrification of mammalian cells |
| US9538745B2 (en) | 2006-04-14 | 2017-01-10 | The General Hospital Corporation | Methods for the cryopreservation of cells |
| WO2007119892A1 (en) * | 2006-04-17 | 2007-10-25 | Chabiotech Co., Ltd. | Methods for vitrification of human oocytes |
| US8435729B2 (en) | 2006-06-12 | 2013-05-07 | The Jackson Laboratory | Sperm cryoprotective media |
| EP2156735A1 (en) * | 2008-08-13 | 2010-02-24 | Dr. H. Zech GmbH | Method and instrument for vitrification and storing of biological specimen |
-
2010
- 2010-08-30 CL CL2010000920A patent/CL2010000920A1/es unknown
-
2011
- 2011-02-16 CA CA2809571A patent/CA2809571A1/en not_active Abandoned
- 2011-02-16 AU AU2011298099A patent/AU2011298099A1/en not_active Abandoned
- 2011-02-16 DK DK11713518.6T patent/DK2611290T3/da active
- 2011-02-16 ES ES11713518.6T patent/ES2633123T3/es active Active
- 2011-02-16 WO PCT/IB2011/050651 patent/WO2012028967A2/en active Application Filing
- 2011-02-16 EP EP11713518.6A patent/EP2611290B1/en active Active
- 2011-02-16 US US13/820,142 patent/US9005886B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012028967A9 (en) | 2012-12-27 |
| ES2633123T3 (es) | 2017-09-19 |
| WO2012028967A2 (en) | 2012-03-08 |
| WO2012028967A3 (en) | 2012-09-13 |
| CA2809571A1 (en) | 2012-03-08 |
| EP2611290B1 (en) | 2017-04-12 |
| AU2011298099A1 (en) | 2013-03-14 |
| EP2611290A2 (en) | 2013-07-10 |
| US9005886B2 (en) | 2015-04-14 |
| US20130157250A1 (en) | 2013-06-20 |
| CL2010000920A1 (es) | 2010-12-17 |
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