DK1133697T3 - Method for modifying and identifying functional sites in proteins - Google Patents
Method for modifying and identifying functional sites in proteinsInfo
- Publication number
- DK1133697T3 DK1133697T3 DK99958116T DK99958116T DK1133697T3 DK 1133697 T3 DK1133697 T3 DK 1133697T3 DK 99958116 T DK99958116 T DK 99958116T DK 99958116 T DK99958116 T DK 99958116T DK 1133697 T3 DK1133697 T3 DK 1133697T3
- Authority
- DK
- Denmark
- Prior art keywords
- tag
- target protein
- proteins
- protein
- functional sites
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6818—Sequencing of polypeptides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
- G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/976—Trypsin; Chymotrypsin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Optics & Photonics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a method for identifying one or more functional sites in proteins. Said method is characterized by the following steps: a) contacting the target protein with a binding partner (BP tag) linked with a laser-activatable marker (tag), resulting in a complex consisting of target protein and BP tag, b) irradiating the complex consisting of target protein and BP tag with laser light, resulting in the formation of free radicals which selectively modify the bound target protein at the binding site, and c) identifying the selectively modified region of the protein by a combination of protein cleavage and mass spectrometry. The invention further relates to a device for carrying out the inventive method.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19854196A DE19854196C2 (en) | 1998-11-24 | 1998-11-24 | Process for the modification and identification of functional sites in proteins |
PCT/EP1999/009052 WO2000031544A1 (en) | 1998-11-24 | 1999-11-23 | Method for modifying and identifying functional sites in proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
DK1133697T3 true DK1133697T3 (en) | 2002-12-02 |
Family
ID=7888853
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK99958116T DK1133697T3 (en) | 1998-11-24 | 1999-11-23 | Method for modifying and identifying functional sites in proteins |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP1133697B1 (en) |
JP (1) | JP2002531810A (en) |
AT (1) | ATE221998T1 (en) |
AU (1) | AU760221B2 (en) |
CA (1) | CA2351667A1 (en) |
DE (2) | DE19854196C2 (en) |
DK (1) | DK1133697T3 (en) |
ES (1) | ES2182584T3 (en) |
PT (1) | PT1133697E (en) |
WO (1) | WO2000031544A1 (en) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE0003566D0 (en) * | 2000-10-02 | 2000-10-02 | Amersham Pharm Biotech Ab | A method for the quantitative determination of one or more compounds |
US6653067B1 (en) * | 2001-02-22 | 2003-11-25 | Isis Pharmaceuticals, Inc. | Methods for identifying ligand binding sites in a biomolecule |
US6858114B2 (en) | 2001-07-03 | 2005-02-22 | The University Of Alabama | Laser hydrolysis of polypeptides |
JP4288571B2 (en) * | 2003-05-21 | 2009-07-01 | 独立行政法人科学技術振興機構 | Method for analyzing physiological function of target substance |
GB2422903A (en) * | 2003-11-21 | 2006-08-09 | Nec Corp | Method of analyzing protein |
KR101708038B1 (en) * | 2014-10-31 | 2017-02-17 | 한국과학기술연구원 | Novel BODIPY compound, method thereof, and method for labelling a protein or a cell by photo-crosslinking |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5565325A (en) * | 1992-10-30 | 1996-10-15 | Bristol-Myers Squibb Company | Iterative methods for screening peptide libraries |
ATE319855T1 (en) * | 1996-12-10 | 2006-03-15 | Sequenom Inc | SEPARABLE, NON-VOLATILE MOLECULES FOR MASS MARKING |
WO1999020190A1 (en) * | 1997-10-23 | 1999-04-29 | President And Fellows Of Harvard College | Laser inactivation of inhibitory molecules in central nervous sy stem myelin |
WO1999041607A2 (en) * | 1998-02-14 | 1999-08-19 | Gmd Forschungszentrum Informationstechnik Gmbh | Fluorescent energy for elucidating the 3-d structure of biological macromolecules |
-
1998
- 1998-11-24 DE DE19854196A patent/DE19854196C2/en not_active Expired - Fee Related
-
1999
- 1999-11-23 CA CA002351667A patent/CA2351667A1/en not_active Abandoned
- 1999-11-23 PT PT99958116T patent/PT1133697E/en unknown
- 1999-11-23 DK DK99958116T patent/DK1133697T3/en active
- 1999-11-23 ES ES99958116T patent/ES2182584T3/en not_active Expired - Lifetime
- 1999-11-23 EP EP99958116A patent/EP1133697B1/en not_active Expired - Lifetime
- 1999-11-23 AT AT99958116T patent/ATE221998T1/en not_active IP Right Cessation
- 1999-11-23 WO PCT/EP1999/009052 patent/WO2000031544A1/en active IP Right Grant
- 1999-11-23 JP JP2000584307A patent/JP2002531810A/en active Pending
- 1999-11-23 AU AU15567/00A patent/AU760221B2/en not_active Ceased
- 1999-11-23 DE DE59902301T patent/DE59902301D1/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
DE19854196C2 (en) | 2001-03-15 |
DE19854196A1 (en) | 2000-08-24 |
EP1133697A1 (en) | 2001-09-19 |
JP2002531810A (en) | 2002-09-24 |
CA2351667A1 (en) | 2000-06-02 |
EP1133697B1 (en) | 2002-08-07 |
ES2182584T3 (en) | 2003-03-01 |
AU760221B2 (en) | 2003-05-08 |
DE59902301D1 (en) | 2002-09-12 |
ATE221998T1 (en) | 2002-08-15 |
AU1556700A (en) | 2000-06-13 |
PT1133697E (en) | 2002-12-31 |
WO2000031544A1 (en) | 2000-06-02 |
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