DK0415296T4 - New highly alkaline proteases - Google Patents

New highly alkaline proteases

Info

Publication number
DK0415296T4
DK0415296T4 DK90116322T DK90116322T DK0415296T4 DK 0415296 T4 DK0415296 T4 DK 0415296T4 DK 90116322 T DK90116322 T DK 90116322T DK 90116322 T DK90116322 T DK 90116322T DK 0415296 T4 DK0415296 T4 DK 0415296T4
Authority
DK
Denmark
Prior art keywords
dna sequences
amino acids
alkaline proteases
alkaline
point mutation
Prior art date
Application number
DK90116322T
Other languages
Danish (da)
Other versions
DK0415296T3 (en
Inventor
Roman Vetter
Detlef Wilke
Andre Clippe
Wolfgang Aehle
Dietmar Schomburg
Antoine Amory
Original Assignee
Biotechnolog Forschung Gmbh
Genencor Internat Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25884604&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK0415296(T4) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Biotechnolog Forschung Gmbh, Genencor Internat Gmbh filed Critical Biotechnolog Forschung Gmbh
Publication of DK0415296T3 publication Critical patent/DK0415296T3/en
Application granted granted Critical
Publication of DK0415296T4 publication Critical patent/DK0415296T4/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Abstract

Optimised high-alkaline proteases which are suitable for use in detergent formulations are described. They are prepared by employing mutated DNA sequences of transformed microorganisms. These DNA sequences are obtained by starting from DNA sequences which code for the high-alkaline protease which is normally produced by Bacillus species, modifying these DNA sequences in defined positions by directed mutagenesis (point mutation) in such a way that the codon in which the point mutation is located now codes for an amino acid which is more strongly basic than the original amino acid. The original amino acids have been replaced in the resulting high-alkaline proteases by more strongly basic amino acids, preferably by the amino acids lysine or arginine. Synthetic oligonucleotides, DNA sequences, vectors and transformed microorganisms used to generate and obtain the optimised high-alkaline protease are likewise described.
DK90116322T 1989-08-31 1990-08-25 New highly alkaline proteases DK0415296T4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3928804 1989-08-31
DE4023458A DE4023458A1 (en) 1989-08-31 1990-07-24 NEW HIGH ALKALINE PROTEASES

Publications (2)

Publication Number Publication Date
DK0415296T3 DK0415296T3 (en) 1996-11-18
DK0415296T4 true DK0415296T4 (en) 2001-11-12

Family

ID=25884604

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90116322T DK0415296T4 (en) 1989-08-31 1990-08-25 New highly alkaline proteases

Country Status (7)

Country Link
EP (1) EP0415296B2 (en)
JP (1) JPH03139277A (en)
KR (1) KR100196785B1 (en)
AT (1) ATE143689T1 (en)
DE (2) DE4023458A1 (en)
DK (1) DK0415296T4 (en)
ES (1) ES2093624T5 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
DE69128094T2 (en) * 1990-12-21 1998-03-19 Novo Nordisk As ENZYME MUTANTS WITH A LOW VARIATION OF MOLECULAR CHARGE OVER A PH RANGE
DE4106525A1 (en) * 1991-03-01 1992-09-10 Solvay Enzymes Gmbh & Co Kg METHOD FOR IMPROVING THE STABILITY OF HIGH ALKALINE PROTEASES
DK58391D0 (en) * 1991-04-03 1991-04-03 Novo Nordisk As HIS UNKNOWN PROTEAS
DE4224125A1 (en) * 1991-07-27 1993-01-28 Solvay Enzymes Gmbh & Co Kg METHOD FOR IMPROVING THE STABILITY OF ENZYMS AND STABILIZED ENZYMES
ATE241704T1 (en) * 1991-08-27 2003-06-15 Hoffmann La Roche PRIMERS AND BUT FOR DETECTING HEPATITIS C
US5371198A (en) * 1991-12-16 1994-12-06 Novo Nordisk A/S Method for protection of proteolysis-susceptible protein during protein production in a fluid medium
US5256557A (en) * 1991-12-27 1993-10-26 Solvay Enzymes, Inc. Purified alkaline protease concentrate and method of preparation
US5623059A (en) * 1992-03-09 1997-04-22 Novo Nordisk A/S Method for protection of proteolysis-susceptible protein during protein production in a fluid medium
EP0571014B1 (en) * 1992-05-18 2004-03-31 Genencor International, Inc. Bacteria producing alkaline proteases, and production of these alkaline proteases
PL178045B1 (en) * 1992-07-17 2000-02-29 Genencor Int Highly alkaline serine proteases
DE4304161A1 (en) * 1993-02-12 1994-08-25 Solvay Enzymes Gmbh & Co Kg Improved highly alkaline proteases
DE4411223A1 (en) * 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Use of alkaline proteases in commercial textile washing processes
EP2258836B1 (en) 2004-09-10 2016-05-04 Novozymes North America, Inc. Methods for preventing, removing, reducing, or disrupting biofilm
PL2566960T3 (en) * 2010-05-06 2017-08-31 The Procter And Gamble Company Consumer products with protease variants
EP2588665B1 (en) 2010-07-01 2019-05-08 Novozymes A/S Bleaching of pulp
WO2012151534A1 (en) 2011-05-05 2012-11-08 Danisco Us Inc. Compositions and methods comprising serine protease variants
US9284687B2 (en) 2011-09-09 2016-03-15 Novozymes A/S Properties of paper materials
US10047352B2 (en) 2012-10-03 2018-08-14 Agrivida, Inc. Intein-modified proteases, their production and industrial applications
EP2740840A1 (en) 2012-12-07 2014-06-11 Novozymes A/S Improving drainage of paper pulp
WO2016007309A1 (en) 2014-07-07 2016-01-14 Novozymes A/S Use of prehydrolysate liquor in engineered wood
WO2021018751A1 (en) 2019-07-26 2021-02-04 Novozymes A/S Enzymatic treatment of paper pulp
CA3182928A1 (en) 2020-05-29 2021-12-02 Novozymes A/S Method for controlling slime in a pulp or paper making process
WO2022263553A1 (en) 2021-06-16 2022-12-22 Novozymes A/S Method for controlling slime in a pulp or paper making process
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3770587A (en) * 1971-02-09 1973-11-06 Pfizer Chemically modified proteolytic enzymes
NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
FR2598430B1 (en) * 1986-05-06 1990-02-02 Roussel Uclaf NOVEL EXPRESSION VECTORS, PROCESS FOR OBTAINING SAME, AND APPLICATION THEREOF FOR EXPRESSING ANY PROTEIN IN ESCHERICHIA COLI
DK6488D0 (en) * 1988-01-07 1988-01-07 Novo Industri As ENZYMES
CN1056187C (en) * 1988-02-11 2000-09-06 金克克国际有限公司 Proteolytic enzymes and their use in detergents
DK316989D0 (en) * 1989-06-26 1989-06-26 Novo Nordisk As ENZYMES

Also Published As

Publication number Publication date
ES2093624T3 (en) 1997-01-01
ES2093624T5 (en) 2001-08-16
KR910004806A (en) 1991-03-29
DE59010522D1 (en) 1996-11-07
EP0415296B2 (en) 2001-04-25
DK0415296T3 (en) 1996-11-18
ATE143689T1 (en) 1996-10-15
JPH03139277A (en) 1991-06-13
EP0415296A2 (en) 1991-03-06
DE4023458A1 (en) 1991-03-07
EP0415296B1 (en) 1996-10-02
EP0415296A3 (en) 1991-10-02
KR100196785B1 (en) 1999-06-15

Similar Documents

Publication Publication Date Title
DK0415296T3 (en) New highly alkaline proteases
JP4086207B2 (en) Subtilisin mutant
DE69635444D1 (en) CLEANED COPRINUS LACCASES AND NUCLEIC ACIDS THAT CODE FOR THIS
NO20050998L (en) DNA encoding a biologically active fragment of bactericidal / premeability-increasing protein process for its preparation, as well as a pharmaceutical preparation containing this
BR9007477A (en) MUTANT SUBTILISIN PROTEASE AND PROCESS FOR DETERMINING OR SELECTING THE POSITION (S) AND THE AMINO ACID (S) TO REMOVE, REPLACE OR INSERT IN THE PLACE OF THE MAINE ENZYME (S)
ATE168130T1 (en) STABILIZED ENZYMES AND DETERGENT COMPOSITIONS
ES2125224T3 (en) SUBTILISIN MUTANTS.
ID27693A (en) NUCLEOTIDE CIRCUITS THAT encode PCK GEN
EA200100419A1 (en) TRANSFORMATION SYSTEM IN THE FIELD OF MUSHROOMS GIFOMYCET-HOSTS
KR930703438A (en) enzyme mutants with low molecular potential variability over a range of pH
DK1294888T3 (en) Interferon-like protein Zcyto21
AU1003888A (en) Pancreatic secretory trypsin inhibitor and variants thereof produced by a recombinant host, process, expression vector and recombinant host therefore and pharmaceutical use thereof
BR9104613A (en) PROTEASE, DNA SEQUENCE, EXPRESSION VECTOR, TRANSFORMANT, PROTEASE PRODUCTION METHOD
DK0870833T3 (en) Genes for ultrathermostable proteases
FI931634A0 (en) NYTT SVAMPPROTEAS
NO944181D0 (en) Proteases for mushrooms
AU665935B2 (en) Thrombolytic peptide, production thereof and thrombolytic agent
MX9401068A (en) VERY ALKALINE PROTEASE IMPROVEMENTS THAT PRESENT A SEQUENCE OF AMINO ACIDS.
Vetter et al. Highly alkaline proteases
EP0945512A3 (en) Novel serine proteases
AU5779690A (en) Mutation of the rhinovirus system