DE69627056D1 - Method of identifying and isolating a molecule - Google Patents

Method of identifying and isolating a molecule

Info

Publication number
DE69627056D1
DE69627056D1 DE69627056T DE69627056T DE69627056D1 DE 69627056 D1 DE69627056 D1 DE 69627056D1 DE 69627056 T DE69627056 T DE 69627056T DE 69627056 T DE69627056 T DE 69627056T DE 69627056 D1 DE69627056 D1 DE 69627056D1
Authority
DE
Germany
Prior art keywords
host cells
molecule
identifying
lysates
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69627056T
Other languages
German (de)
Other versions
DE69627056T2 (en
Inventor
Michael Pfreundschuh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ludwig Institute for Cancer Research New York
Original Assignee
Ludwig Institute for Cancer Research New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/580,980 external-priority patent/US6025191A/en
Priority claimed from US08/644,116 external-priority patent/US6140464A/en
Application filed by Ludwig Institute for Cancer Research New York filed Critical Ludwig Institute for Cancer Research New York
Publication of DE69627056D1 publication Critical patent/DE69627056D1/en
Application granted granted Critical
Publication of DE69627056T2 publication Critical patent/DE69627056T2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4748—Tumour specific antigens; Tumour rejection antigen precursors [TRAP], e.g. MAGE
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57438—Specifically defined cancers of liver, pancreas or kidney
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

The invention describes methods for identifying a molecule of interest, as well as nucleic acid molecules which encode it, and binding partners for it. A cDNA library from a cell expressing the target is prepared, and expressed in host cells. Lysates of the host cells are screened with a sample, treated to remove interfering binding partners. The treatment involves contact of the sample to lysates of untransfected host cells, and host cells transfected or transformed with the same vector used to make the cDNA library. Also a part of the invention are antigens and cDNA identified using the methodology.
DE69627056T 1995-06-07 1996-06-07 Method of identifying and isolating a molecule Expired - Lifetime DE69627056T2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/479,328 US5698396A (en) 1995-06-07 1995-06-07 Method for identifying auto-immunoreactive substances from a subject
US08/580,980 US6025191A (en) 1995-06-07 1996-01-03 Isolated nucleic acid molecules which encode a melanoma specific antigen and uses thereof
US08/644,116 US6140464A (en) 1995-06-07 1996-05-10 Nonapeptides that bind a HLA-A2.1 molecule

Publications (2)

Publication Number Publication Date
DE69627056D1 true DE69627056D1 (en) 2003-04-30
DE69627056T2 DE69627056T2 (en) 2004-01-29

Family

ID=23903564

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69627056T Expired - Lifetime DE69627056T2 (en) 1995-06-07 1996-06-07 Method of identifying and isolating a molecule
DE69623014T Expired - Lifetime DE69623014T2 (en) 1995-06-07 1996-06-07 METHOD FOR IDENTIFYING AND ISOLATING A MOLECULE AND MOLECULE DETECTED BY IT

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69623014T Expired - Lifetime DE69623014T2 (en) 1995-06-07 1996-06-07 METHOD FOR IDENTIFYING AND ISOLATING A MOLECULE AND MOLECULE DETECTED BY IT

Country Status (11)

Country Link
US (2) US5698396A (en)
EP (1) EP0831879B1 (en)
JP (1) JP2002502354A (en)
CN (1) CN1136914C (en)
AT (2) ATE222116T1 (en)
CA (1) CA2223566C (en)
DE (2) DE69627056T2 (en)
DK (1) DK0831879T3 (en)
ES (1) ES2180782T3 (en)
PT (1) PT831879E (en)
WO (1) WO1996040209A1 (en)

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Also Published As

Publication number Publication date
DE69623014T2 (en) 2003-05-08
US5698396A (en) 1997-12-16
AU6165296A (en) 1996-12-30
JP2002502354A (en) 2002-01-22
US5798264A (en) 1998-08-25
EP0831879A4 (en) 2000-01-26
WO1996040209A1 (en) 1996-12-19
PT831879E (en) 2002-12-31
ATE235248T1 (en) 2003-04-15
AU704109B2 (en) 1999-04-15
CN1190894A (en) 1998-08-19
CA2223566A1 (en) 1996-12-19
CN1136914C (en) 2004-02-04
CA2223566C (en) 2002-09-03
EP0831879A1 (en) 1998-04-01
DE69627056T2 (en) 2004-01-29
DE69623014D1 (en) 2002-09-19
EP0831879B1 (en) 2002-08-14
ES2180782T3 (en) 2003-02-16
ATE222116T1 (en) 2002-08-15
DK0831879T3 (en) 2002-12-16

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