DE69434242D1 - Novel lipase variants for use in detergents - Google Patents

Novel lipase variants for use in detergents

Info

Publication number
DE69434242D1
DE69434242D1 DE69434242T DE69434242T DE69434242D1 DE 69434242 D1 DE69434242 D1 DE 69434242D1 DE 69434242 T DE69434242 T DE 69434242T DE 69434242 T DE69434242 T DE 69434242T DE 69434242 D1 DE69434242 D1 DE 69434242D1
Authority
DE
Germany
Prior art keywords
detergents
lipase variants
methionine
novel lipase
lipases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69434242T
Other languages
German (de)
Other versions
DE69434242T2 (en
Inventor
Der Laan Metske Van
Bernardus Lenting
Johannes Mulleners
Mathilde Cox
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Application granted granted Critical
Publication of DE69434242D1 publication Critical patent/DE69434242D1/en
Publication of DE69434242T2 publication Critical patent/DE69434242T2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase

Abstract

Modified lipases are disclosed whereby at least the methionine at a position corresponding to position 21 in wild-type Pseudomonas pseudoalcaligenes lipase is substituted by another amino acid, which lipases exhibit a desired property change, in particular improved wash performance. Preferred embodiments are substitutions of said methionine by leucine, serine or alanine.
DE69434242T 1993-04-27 1994-04-27 Novel lipase variants for use in detergents Expired - Lifetime DE69434242T2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP93201212 1993-04-27
EP93201212 1993-04-27
PCT/EP1994/001435 WO1994025578A1 (en) 1993-04-27 1994-04-27 New lipase variants for use in detergent applications

Publications (2)

Publication Number Publication Date
DE69434242D1 true DE69434242D1 (en) 2005-03-03
DE69434242T2 DE69434242T2 (en) 2006-01-12

Family

ID=8213797

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69434242T Expired - Lifetime DE69434242T2 (en) 1993-04-27 1994-04-27 Novel lipase variants for use in detergents

Country Status (12)

Country Link
EP (1) EP0652946B1 (en)
JP (1) JP3618748B2 (en)
KR (1) KR950702240A (en)
CN (1) CN1108457A (en)
AT (1) ATE287946T1 (en)
AU (1) AU673078B2 (en)
CA (1) CA2138519C (en)
DE (1) DE69434242T2 (en)
DK (1) DK0652946T3 (en)
FI (1) FI116728B (en)
PL (1) PL306812A1 (en)
WO (1) WO1994025578A1 (en)

Families Citing this family (562)

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EP0652946A1 (en) 1995-05-17
PL306812A1 (en) 1995-04-18
AU6723394A (en) 1994-11-21
CN1108457A (en) 1995-09-13
DE69434242T2 (en) 2006-01-12
JPH07508659A (en) 1995-09-28
EP0652946B1 (en) 2005-01-26
DK0652946T3 (en) 2005-05-30
CA2138519A1 (en) 1994-11-10
JP3618748B2 (en) 2005-02-09

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