DE618789C - Process for obtaining physiologically active substances from the anterior pituitary gland - Google Patents
Process for obtaining physiologically active substances from the anterior pituitary glandInfo
- Publication number
- DE618789C DE618789C DEI35149D DEI0035149D DE618789C DE 618789 C DE618789 C DE 618789C DE I35149 D DEI35149 D DE I35149D DE I0035149 D DEI0035149 D DE I0035149D DE 618789 C DE618789 C DE 618789C
- Authority
- DE
- Germany
- Prior art keywords
- active substances
- anterior pituitary
- pituitary gland
- physiologically active
- obtaining physiologically
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims description 6
- 210000004198 Pituitary Gland, Anterior Anatomy 0.000 title claims description 4
- 239000000126 substance Substances 0.000 title claims description 4
- 230000029087 digestion Effects 0.000 claims description 4
- 206010057248 Cell death Diseases 0.000 claims description 3
- 210000004907 Glands Anatomy 0.000 claims description 3
- 239000006286 aqueous extract Substances 0.000 claims description 3
- 239000003125 aqueous solvent Substances 0.000 claims description 3
- 230000018867 autolysis Effects 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 239000003960 organic solvent Substances 0.000 claims description 3
- 230000028043 self proteolysis Effects 0.000 claims description 3
- 230000004048 modification Effects 0.000 claims 1
- 238000006011 modification reaction Methods 0.000 claims 1
- CSCPPACGZOOCGX-UHFFFAOYSA-N acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 239000000284 extract Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229940055729 Papain Drugs 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000002349 favourable Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000000977 initiatory Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 230000001817 pituitary Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002335 preservative Effects 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000003799 water insoluble solvent Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
Description
Verfahren zur Gewinnung physiologisch wirksamer Stoffe aus Hypophysenvorderlappen In. dem Patent 614861 ist ein Verfahren zur Gewinnung von physiologisch wirksamen Stoffen aus Hypophysenvorderlappen beschrieben, welches darin besteht, daß die Drüsen einer kombinierten Behandlung mit organischen und wässerrigen Lösungsmitteln unterwoifen werden.Process for the extraction of physiologically active substances from the anterior pituitary gland In. the patent 614861 is a method for the production of physiologically active Substances from the anterior pituitary gland described, which consists in the glands subject to a combined treatment with organic and aqueous solvents will.
Brei weiterer Bearbeitung des Verfahrens wurde nun gefunden, daß man zu günstigeren Resultaten, namentlich bezüglich der Stärke der wässerigen Extrakte, gelangen kann, wenn man die Drüsen nach erfolgter Behandlung mit organischen Lösungsmitteln bei der anschließenden Extraktion mit wässerigen Lösungsmitteln gleichzeitig einem Autolyse- bzw. Verdauungsprozeß und gegebenenfalls die erhaltenen wässerigen. Extrakte einer Nachbehandlung nach dem Verfahren des Hauptpatents unterwirft. Die letztere Maßnahme ist besonders angezeigt, um bei der Autolyse oder Verdauung evtl. entstandene Hemmungskörper zu entfernen.Much further processing of the process has now been found that one to more favorable results, especially with regard to the strength of the aqueous extracts, can get if the glands after treatment with organic solvents in the subsequent extraction with aqueous solvents simultaneously one Autolysis or digestion process and optionally the aqueous ones obtained. Extracts subject to post-treatment according to the procedure of the main patent. The latter Measure is particularly indicated to avoid any resulting during autolysis or digestion Remove the inhibitor.
Die wässerigen Extrakte können dann durch Weiterbehandlung mit wasserunlöslichen Lösungsmitteln von Ballaststoffen, wie Lipoiden usw., befreit werden. Beispiele i. io g vorbehandelte Hypophysenvorderlappen werden mit i oö ccm Wasser unter Zugabe eines Konservierun.gsmitt@els bei 2o bis 25° C mehrere Tage autolysiert, das Ganze zweimal mit je der doppelten Menge Aceton versetzt rund das. Gesamtextrakt im Vakuum von Aceton befreit. Der wässerige Anteil wird mit Äther ausgeschüttelt und nach Entfernung des Äthers am Tier ausgewertet.The aqueous extracts can then be treated with water-insoluble Solvents of dietary fiber, such as lipoids, etc., can be freed. Examples i. 10 g pretreated anterior pituitary lobes are mixed with 100 cc of water with the addition of a Conservierun.gsmitt@els autolyzed at 2o to 25 ° C for several days, the whole thing twice with twice the amount of acetone added around the total extract in vacuo freed from acetone. The watery part is shaken out with ether and after Evaluation of the removal of the ether on the animal.
2. 5 g Trockenpräparat werden mit o,5 g Papain (i :8o) und 5o ccm Wasser mit oder ohne Pufferlösung unter Zusatz eines Konservierungsmittels (nach Einleitung der Verdauung bei erhöhter Temperatur) mehrere Tage bei 20° C verdaut. Durch scharfes Zentrifugieren erhält man eine klare Lösung mit geringem Eiweißgehalt, die am Tier ausgewertet rund gegebenenfalls mach Beispiel i weiter gereinigt werden kann.2. 5 g dry preparation are mixed with 0.5 g papain (i: 8o) and 50 ccm Water with or without a buffer solution with the addition of a preservative (according to Initiation of digestion at elevated temperature) digested for several days at 20 ° C. Sharp centrifugation gives a clear solution with a low protein content, which are evaluated on the animal and possibly further cleaned according to example i can.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEI35149D DE618789C (en) | 1928-08-05 | 1928-08-05 | Process for obtaining physiologically active substances from the anterior pituitary gland |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEI35149D DE618789C (en) | 1928-08-05 | 1928-08-05 | Process for obtaining physiologically active substances from the anterior pituitary gland |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE618789C true DE618789C (en) | 1935-09-14 |
Family
ID=7188905
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DEI35149D Expired DE618789C (en) | 1928-08-05 | 1928-08-05 | Process for obtaining physiologically active substances from the anterior pituitary gland |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE618789C (en) |
-
1928
- 1928-08-05 DE DEI35149D patent/DE618789C/en not_active Expired
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