DE487199C - Process for the preparation of purified tuberculin preparations - Google Patents
Process for the preparation of purified tuberculin preparationsInfo
- Publication number
- DE487199C DE487199C DEI30286D DEI0030286D DE487199C DE 487199 C DE487199 C DE 487199C DE I30286 D DEI30286 D DE I30286D DE I0030286 D DEI0030286 D DE I0030286D DE 487199 C DE487199 C DE 487199C
- Authority
- DE
- Germany
- Prior art keywords
- preparations
- tuberculin
- preparation
- purified tuberculin
- active substances
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 229960001005 tuberculin Drugs 0.000 title claims description 12
- 238000002360 preparation method Methods 0.000 title claims description 10
- 238000000034 method Methods 0.000 title claims description 3
- 239000013543 active substance Substances 0.000 claims description 5
- 201000008827 tuberculosis Diseases 0.000 claims description 3
- 229930013930 alkaloid Natural products 0.000 claims description 2
- 150000003797 alkaloid derivatives Chemical class 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 239000000243 solution Substances 0.000 description 9
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229940046892 lead acetate Drugs 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/04—Mycobacterium, e.g. Mycobacterium tuberculosis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Communicable Diseases (AREA)
- Pulmonology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Description
Verfahren zur Darstellung von gereinigten Tuberkulinpräparaten Die für die Therapie und Diagnose der Tuberkulose verwendeten Tuberkulinpräparate enthalten mehr oder weniger große Mengen Eiweiß. sowie Eiweißabbauprodukte, Salze und andere Verunreinigungen, welche den Bakterien oder den für die Züchtung der Bakterien verwendeten Nährlösungen entstammen. Diese Stoffe, welche an sich als unspezifisch anzusehen sind, können bekanntlich die bei der Tuberkulose angewandte Cutanreaktion in positivem Sinne begünstigen oder vortäuschen, wodurch der Wert dieser Reaktion naturgemäß sehr in Frage gestellt ist, außerdem bei der Therapie störende Nebenwirkungen hervorrufen, welche die gewünschte reine Tuberkulinwirkung in ungünstigem Sinne beeinflussen. Es ist daher von großem diagnostischem und therapeutischem Wert, Tuberkulinpräparate anzuwenden, welche weitgehendst von diesen unspezifischen Stoffen befreit sind.Process for the preparation of purified tuberculin preparations The Contain tuberculin preparations used for the therapy and diagnosis of tuberculosis more or less large amounts of protein. as well as protein breakdown products, salts and others Impurities used by the bacteria or that used to grow the bacteria Nutrient solutions come from. These substances, which in themselves are to be regarded as unspecific are, as is well known, the cutane reaction used in tuberculosis can be positive Senses favor or pretend, making the value of this response natural is very questionable, also cause disruptive side effects during therapy, which influence the desired pure tuberculin effect in an unfavorable sense. It is therefore of great diagnostic and therapeutic value to use tuberculin preparations to be used, which are largely freed from these unspecific substances.
Es wurde nun gefunden, daß Tuberkulinpräparate beliebiger Herkunft durch Behandlung mit den üblichen Enteiweißungsmhteln, wie Eisendialysat, Bleisalzen und anderen, nichts oder fast nichts von ihrer Wirksamkeit einbüßen, daß vielmehr lediglich die Eiweißstoffe und deren Spaltprodukte gefällt werden. Die so behandelten Lösungen enthalten die wirksamen Bestandteile der Tuberkuline und zeigen keine Biuretreaktion mehr. Weiter wurde gefunden, daß man die in den enteiweißten Lösungen vorhandenen wirksamen Bestandteile dadurch einer weiteren Reinigung unterziehen bzw. die wirksamen Substanzen zur Abscheidung bringen kann, daß man die Lösungen mit Aceton, Alkoholen oder anderen Lösungsmitteln, in denen die wirksamen Substanzen unlöslich sind, behandelt oder daß man die wirksamen Stoffe zunächst mit Fällungsmitteln der Alkaloidreagentiengruppe abscheidet und die erhaltenen Fällungen durch nachträgliches Zerlegen vom Fällungsmittel befreit. Beispiele i. zog humanes Trockentuberkulin, hergestellt aus Kochbouillon durch Alkoholfällung, werden in der Sofachen Menge destilliertem Wasser gelöst und die Lösung so lange mit Eisendialysat versetzt, bis im Filtrat keine .Eiweiß- und Biuretreaktion mehr feststellbar ist. Aus dem eingeengten Filtrat werden die wirksamen Substanzen mit wäßriger Pikrinsäurelösung gefällt und die entstandenen Tuberkulinfällungen abgesaugt und mit salzsäurehaltigem Wasser zerlegt. Aus den entstandenen Lösungen werden die wirksamen Stoffe mit Aceton gefällt. Statt von Trockentuberkulin kann man auch von der entsprechenden Menge Alttuberkulin »Koch« ausgehen. 2. 20 g bovine Tuberkelsäure, hergestellt durch Extraktion entfetteter und entwachster boviner Tuberkelbazillen werden in der Sofachen Menge destilliertem Wasser _ gelöst und die Lösung mit neutralem Bleiacetat enteiweißt. Das Filtrat wird zur Entfernung von noch vorhandenen geringen Mengen Bleisalz mit Schwefelwasserstoff entbleit und das Filtrat zwecks Abscheidung der wirksamen Bestandteile mit Aceton gefällt.It has now been found that tuberculin preparations of any origin by treatment with the usual protein removal agents, such as iron dialysate, lead salts and others, lose nothing or almost nothing of their effectiveness, that rather only the proteins and their breakdown products are precipitated. Those treated like that Solutions contain the active components of the tuberculins and do not show any biuret reaction more. It has also been found that those present in the deproteinized solutions can be used subject the active components to further cleaning or the active components Substances can be brought to the separation that one the solutions with acetone, alcohols or other solvents in which the active substances are insoluble or that the active substances are first treated with precipitants from the alkaloid reagent group separates and the precipitates obtained by subsequent decomposition of the precipitant freed. Examples i. pulled human dry tuberculin, made from cooking broth by alcohol precipitation, are dissolved in twice the amount of distilled water and iron dialysate is added to the solution until there is no protein and protein in the filtrate Biuret reaction is more noticeable. The concentrated filtrate becomes the effective Substances precipitated with aqueous picric acid solution and the resulting tuberculin precipitations sucked off and decomposed with hydrochloric acid-containing water. From the resulting solutions the active substances are precipitated with acetone. Instead of dry tuberculin can one can also start from the corresponding amount of old tuberculin "Koch". 2. 20 g of bovine tubercular acid, produced by extraction of defatted and dewaxed bovine tubercle bacilli are dissolved in twice the amount of distilled water and deproteinize the solution with neutral lead acetate. The filtrate is used for removal defleaded from still existing small amounts of lead salt with hydrogen sulfide and the filtrate is precipitated with acetone to separate the active components.
3. @o g abgetötete Tuberkelbazillen werden mit Alkohol extrahiert und die alkoholische Lösung im Vakuum eingedampft. Der Rückstand, der neben den Tuberkelfetten noch reich an Tuberkulin ist, wird mit Wasser extrahiert und die wäßrige Lösung, wie in Beispiel 2 angegeben, von Ballaststoffen befreit.3. @o g killed tubercle bacilli are extracted with alcohol and the alcoholic solution evaporated in vacuo. The residue that is next to the Tubercle fat is still rich in tuberculin, is extracted with water and the aqueous solution, as indicated in Example 2, freed from dietary fiber.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEI30286D DE487199C (en) | 1927-02-12 | 1927-02-12 | Process for the preparation of purified tuberculin preparations |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEI30286D DE487199C (en) | 1927-02-12 | 1927-02-12 | Process for the preparation of purified tuberculin preparations |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE487199C true DE487199C (en) | 1929-12-03 |
Family
ID=7187578
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DEI30286D Expired DE487199C (en) | 1927-02-12 | 1927-02-12 | Process for the preparation of purified tuberculin preparations |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE487199C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3969497A (en) * | 1971-05-05 | 1976-07-13 | Lincoln Laboratories, Inc. | Test substance for tuberculosis |
-
1927
- 1927-02-12 DE DEI30286D patent/DE487199C/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3969497A (en) * | 1971-05-05 | 1976-07-13 | Lincoln Laboratories, Inc. | Test substance for tuberculosis |
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