DE4434093A1 - Verfahren zum qualitativen oder/und quantitativen Nachweis einer zu bestimmenden Substanz - Google Patents

Verfahren zum qualitativen oder/und quantitativen Nachweis einer zu bestimmenden Substanz

Info

Publication number
DE4434093A1
DE4434093A1 DE4434093A DE4434093A DE4434093A1 DE 4434093 A1 DE4434093 A1 DE 4434093A1 DE 4434093 A DE4434093 A DE 4434093A DE 4434093 A DE4434093 A DE 4434093A DE 4434093 A1 DE4434093 A1 DE 4434093A1
Authority
DE
Germany
Prior art keywords
solid phase
inactivated
active
biotin
substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE4434093A
Other languages
German (de)
English (en)
Inventor
Werner Dr Rer Nat Naser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Priority to DE4434093A priority Critical patent/DE4434093A1/de
Priority to US08/531,838 priority patent/US5935780A/en
Priority to EP95114973A priority patent/EP0703452B1/de
Priority to DE59510661T priority patent/DE59510661D1/de
Priority to ES95114973T priority patent/ES2199233T3/es
Priority to JP7246505A priority patent/JP2902581B2/ja
Publication of DE4434093A1 publication Critical patent/DE4434093A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/969—Multiple layering of reactants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/823—Immunogenic carrier or carrier per se
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/825—Pretreatment for removal of interfering factors from sample

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE4434093A 1994-09-23 1994-09-23 Verfahren zum qualitativen oder/und quantitativen Nachweis einer zu bestimmenden Substanz Withdrawn DE4434093A1 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DE4434093A DE4434093A1 (de) 1994-09-23 1994-09-23 Verfahren zum qualitativen oder/und quantitativen Nachweis einer zu bestimmenden Substanz
US08/531,838 US5935780A (en) 1994-09-23 1995-09-21 Method for the qualitative or/and quantitative detection of an analyte
EP95114973A EP0703452B1 (de) 1994-09-23 1995-09-22 Verfahren zum qualitativen und/oder quantitativen Nachweis einer zu bestimmenden Substanz
DE59510661T DE59510661D1 (de) 1994-09-23 1995-09-22 Verfahren zum qualitativen und/oder quantitativen Nachweis einer zu bestimmenden Substanz
ES95114973T ES2199233T3 (es) 1994-09-23 1995-09-22 Procedimiento para la deteccion cualitativa y cuantitativa de una sustancia que se ha de determinar.
JP7246505A JP2902581B2 (ja) 1994-09-23 1995-09-25 測定すべき物質の定性的または／および定量的検出法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4434093A DE4434093A1 (de) 1994-09-23 1994-09-23 Verfahren zum qualitativen oder/und quantitativen Nachweis einer zu bestimmenden Substanz

Publications (1)

Publication Number Publication Date
DE4434093A1 true DE4434093A1 (de) 1996-03-28

Family

ID=6529055

Family Applications (2)

Application Number Title Priority Date Filing Date
DE4434093A Withdrawn DE4434093A1 (de) 1994-09-23 1994-09-23 Verfahren zum qualitativen oder/und quantitativen Nachweis einer zu bestimmenden Substanz
DE59510661T Expired - Lifetime DE59510661D1 (de) 1994-09-23 1995-09-22 Verfahren zum qualitativen und/oder quantitativen Nachweis einer zu bestimmenden Substanz

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE59510661T Expired - Lifetime DE59510661D1 (de) 1994-09-23 1995-09-22 Verfahren zum qualitativen und/oder quantitativen Nachweis einer zu bestimmenden Substanz

Country Status (5)

Country Link
US (1) US5935780A (https=)
EP (1) EP0703452B1 (https=)
JP (1) JP2902581B2 (https=)
DE (2) DE4434093A1 (https=)
ES (1) ES2199233T3 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0799890A2 (de) 1996-04-01 1997-10-08 Roche Diagnostics GmbH Rekombinante inaktive Core-Streptavidin Mutanten

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6905816B2 (en) 2000-11-27 2005-06-14 Intelligent Medical Devices, Inc. Clinically intelligent diagnostic devices and methods
US20030003464A1 (en) * 2000-11-27 2003-01-02 Phan Brigitte C. Dual bead assays including optical biodiscs and methods relating thereto
US20040248093A1 (en) * 2000-11-27 2004-12-09 Coombs James Howard Magneto-optical bio-discs and systems including related methods
US20020172980A1 (en) * 2000-11-27 2002-11-21 Phan Brigitte Chau Methods for decreasing non-specific binding of beads in dual bead assays including related optical biodiscs and disc drive systems
AU2003211623A1 (en) * 2002-03-04 2003-09-16 The New Industry Research Organization Method of measuring the activity of dna-binding protein
EP1493029B1 (en) * 2002-04-10 2010-03-31 Response Biomedical Corporation Sensitive immunochromatographic assay
US7175992B2 (en) * 2002-04-10 2007-02-13 Response Biomedical Corporation Sensitive immunochromatographic assay
US20030198967A1 (en) * 2002-04-23 2003-10-23 Matson Robert S. Multi-functional microarrays and methods
US20050014249A1 (en) * 2003-02-21 2005-01-20 Norbert Staimer Chromatographic analysis on optical bio-discs and methods relating thereto
WO2008106021A1 (en) * 2007-02-26 2008-09-04 Response Biomedical Corporation Comparative multiple analyte assay
US20120064543A1 (en) 2009-03-02 2012-03-15 Virus Ikagaku Kenkyusho Inc. Method for detecting substance in biological sample
AU2010232305B2 (en) 2009-03-31 2015-03-05 Japan Tobacco Inc. Method for detecting substance in biological sample
US20130052669A1 (en) * 2010-04-30 2013-02-28 Amanda G. Paulovich Compositions and methods for reliably detecting and/or measuring the amount of a modified target protein in a sample
WO2013018836A1 (ja) * 2011-08-01 2013-02-07 日本たばこ産業株式会社 生物学的試料中の物質を検出する工程において、非特異的結合を抑制する方法、当該方法に使用するための剤
JP6765232B2 (ja) * 2016-06-30 2020-10-07 シスメックス株式会社 免疫複合体転移法により被検物質を検出するための抗体試薬及びその製造方法、並びにその抗体試薬の利用
CN114910635B (zh) * 2017-10-26 2025-05-02 科美诊断技术股份有限公司 一种无基质效应的均相免疫检测试剂盒及其应用
WO2019183334A1 (en) 2018-03-21 2019-09-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
AU2020308574A1 (en) * 2019-06-25 2022-03-24 Veravas, Inc. Compositions and methods for detecting and depleting sample interferences
CN116539866A (zh) * 2019-07-19 2023-08-04 科美博阳诊断技术(上海)有限公司 一种均相化学发光分析的方法及其应用
CN111812336A (zh) * 2020-08-10 2020-10-23 苏州康和顺医疗技术有限公司 用于检测冠状病毒抗体的检测试剂盒及其制备方法
CN121741173A (zh) * 2024-09-25 2026-03-27 中元汇吉生物技术股份有限公司 全液相反应层析检测试剂、方法及系统

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL51667A (en) * 1977-03-16 1979-10-31 Miles Yeda Ltd Immunoassay for the determination of a hapten
US4294817A (en) * 1977-11-25 1981-10-13 International Diagnostic Technology, Inc. Method of fluoro immunoassay
JPS5510590A (en) * 1978-05-04 1980-01-25 Wellcome Found Enzyme immunity quantity analysis
US4228237A (en) * 1978-09-21 1980-10-14 Calbiochem-Behring Corp. Methods for the detection and determination of ligands
US4243655A (en) * 1979-09-04 1981-01-06 Gunther Roland E Dental health-care aids
US4656252A (en) * 1980-01-24 1987-04-07 Giese Roger W Amidobiotin compounds useful in a avidin-biotin multiple layering process
NL8102178A (nl) * 1981-05-02 1982-12-01 Stichting Centraal Lab Werkwijze voor het aantonen van tegen bepaalde antigenen gerichte antistoffen, alsmede inrichting en reagentia voor het uitvoeren van deze werkwijze.
JPS59143960A (ja) * 1983-02-08 1984-08-17 Amano Pharmaceut Co Ltd 酵素標識抗体に含まれる非特異的吸着成分の除去法
US4490473A (en) * 1983-03-28 1984-12-25 Panab Labeled antibodies and methods
JPS60242369A (ja) * 1984-05-16 1985-12-02 Fujirebio Inc イムノアツセイにおける非特異反応吸収剤
JPS61250559A (ja) * 1985-04-30 1986-11-07 Toshiba Corp 免疫分析方法
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays
DE3705686C2 (de) * 1987-02-23 1995-11-30 Boehringer Mannheim Gmbh Verfahren zur Bestimmung von Antikörpern
DE3720983A1 (de) * 1987-06-25 1989-01-05 Hoechst Ag Immunometrisches bestimmungsverfahren
US5268306A (en) * 1988-02-29 1993-12-07 Boehringer Mannheim Gmbh Preparation of a solid phase matrix containing a bound specific binding pair
DE3806431A1 (de) * 1988-02-29 1989-09-07 Boehringer Mannheim Gmbh Verfahren zur herstellung einer festphasenmatrix
US5277589A (en) * 1988-07-08 1994-01-11 Boehringer Mannheim Gmbh Process for the determination of antibodies
US5096837A (en) * 1990-02-08 1992-03-17 Pacific Biotech, Inc. Immunochromatographic assay and method of using same
DE4024544A1 (de) * 1990-08-02 1992-02-06 Boehringer Mannheim Gmbh Analyseelement und verfahren zu seiner herstellung
DE4134833A1 (de) * 1991-09-25 1993-04-01 Boehringer Mannheim Gmbh Verfahren zur bestimmung von fibrin
US5656504A (en) * 1992-10-26 1997-08-12 Pharmacia Biosensor Ab Method of preventing undesired binding in solid phase assays
WO1995023801A1 (de) * 1994-03-05 1995-09-08 Boehringer Mannheim Gmbh Entstörmittel zum einsatz bei immunoassays

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0799890A2 (de) 1996-04-01 1997-10-08 Roche Diagnostics GmbH Rekombinante inaktive Core-Streptavidin Mutanten

Also Published As

Publication number Publication date
EP0703452A3 (https=) 1996-05-01
EP0703452A2 (de) 1996-03-27
JP2902581B2 (ja) 1999-06-07
ES2199233T3 (es) 2004-02-16
EP0703452B1 (de) 2003-05-02
JPH08114590A (ja) 1996-05-07
DE59510661D1 (de) 2003-06-05
US5935780A (en) 1999-08-10

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Legal Events

Date Code Title Description
8127 New person/name/address of the applicant

Owner name: ROCHE DIAGNOSTICS GMBH, 68305 MANNHEIM, DE

8110 Request for examination paragraph 44
8139 Disposal/non-payment of the annual fee