DE3700122A1 - Chamber for separating a particle mixture according to the countercurrent centrifugation method - Google Patents

Chamber for separating a particle mixture according to the countercurrent centrifugation method

Info

Publication number
DE3700122A1
DE3700122A1 DE19873700122 DE3700122A DE3700122A1 DE 3700122 A1 DE3700122 A1 DE 3700122A1 DE 19873700122 DE19873700122 DE 19873700122 DE 3700122 A DE3700122 A DE 3700122A DE 3700122 A1 DE3700122 A1 DE 3700122A1
Authority
DE
Grant status
Application
Patent type
Prior art keywords
chamber
particles
separating
separation
flow
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19873700122
Other languages
German (de)
Inventor
Manfred Lutz
Wolfgang Dr Rer Nat Hartmann
Gerhard Dr Med Gaedicke
Original Assignee
Manfred Lutz
Hartmann Wolfgang
Gerhard Dr Med Gaedicke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/04Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/06Centrifugal counter-current apparatus
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/04Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B04B2005/0471Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation with additional elutriation separation of different particles

Abstract

In counterstream centrifugation technology, in addition to the centrifugal force, forces of a centripetally directed flow of liquid act on a mixture of particles. An equilibrium of forces is established as a function of the size and density of the particles. By changing the force ratios, particles of different sizes and density can be isolated from the liquid flowing off in centripetal fashion. The geometric profile of the separating area, the so-called separating chamber, is decisive for the selectivity of separation. By virtue of eddy effects of the liquid flow and by virtue of non-uniform distribution of flow, the separation selectivity is unfavourably influenced in previously known separating chambers. In the described separating chamber, a uniform flow behaviour and a uniform distribution of the particles in the separating chamber are achieved by means of a central inlet opening which is aligned in the longitudinal direction of the chamber and by means of a constantly increasing cross-section of the chamber. As a result, the precise, non-damaging separation of large amounts of a mixture of living cells is made possible.

Description

Aus einem Gemisch von Teilchen sollen einzelne Fraktionen in möglichst großer Reinheit gewonnen werden. single fractions to be obtained in the highest possible purity from a mixture of particles.

PE Lindahl hat (Nature 161: 648-649; 1948) eine Technik beschrieben, bei der in einem Zentrifugen rotor zusätzlich zu der auf Teilchen wirkenden Zentrifugalkraft eine zentripetale Flüssigkeits strömung wirkt. PE has Lindahl (Nature 161: 648-649; 1948) described a technique in which in a centrifuge rotor in addition to the centrifugal force acting on particles of a centripetal liquid flow acts. Abhängig von Größe und Dichte der Teilchen bildet sich ein Gleichgewicht beider Kräfte. Depending on the size and density of particles, an equilibrium of both forces forming. Aus der nach zentral abfließenden Flüs sigkeit lassen sich durch Änderung von Rotorge schwindigkeit beziehungsweise Flüssigkeitsströmung Fraktionen unterschiedlicher Größe und Dichte ge winnen. sigkeit blank from the effluent to central flues at the speed by changing Rotorge or liquid flow fractions of different size and density ge winnen. Maßgeblich für die Schärfe der Trennung ist das geometrische Profil des Trennbereichs, die sogenannte Trennkammer. Decisive for the sharpness of the separation is the geometric profile of the separation area, the so-called separation chamber.

In Patent 43 50 283 wird von BECKMAN Instru ments ein Rotor mit Trennkammer beschrieben. In Patent 43 50 283 a rotor is described with the separation chamber of BECKMAN Instru ments.

In dieser Trennkammer treten unregelmäßige Strö mungsverteilungen und Verwirbelungen der Flüssig keitsströme auf. In this separation chamber irregular Strö occur mung distributions and turbulence of the liquid keitsströme on. Dies führt zu ungleichmäßiger Teilchenverteilung, zu unscharfer Trennung und bei lebenden Zellen zu Verklumpung und Zellzerstörung. This leads to uneven particle distribution to blurred separation and in living cells clumping and cell destruction.

Die Erfindung soll durch ein geeignetes Kammerpro fil den Strömungsverlauf gleichmäßig gestalten, Verwirbelungen vermindern und die effektive Trenn strecke vergrößern. The invention is to make the flow pattern uniformly reduce turbulence and the effective separation distance larger by a suitable Kammerpro fil. Es wird dadurch eine schonen dere und präzisere Trennung ermöglicht. It is made possible by a spare breadth and accuracy of separation. Gleichzei tig steigt die maximal mögliche Beladbarkeit mit Teilchen. Gleichzei tig the maximum possible loading capacity increases with particles.

Die Aufgabe wird erfindungsgemäß dadurch gelöst, daß eine großvolumige, zentrale, in der Längs achse ausgerichtete Einlaßöffnung ( 1 ) und ein sich konstant vergrößernder Querschnitt ( 2 ) sich dergestalt ergänzen, daß ein gleichmäßiger Strö mungsverlauf und eine gleichmäßige Verteilung der Teilchen erreicht wird. The object is inventively achieved in that a large volume, a central, aligned in the longitudinal axis of the inlet opening (1) and a constantly enlarging cross-section (2) complement each other such that a uniform Strö mung extending and a uniform distribution of the particles is achieved.

Die Trennschärfe wird erhöht, die Teilchen schonen der getrennt und die maximal mögliche Beladung mit Teilchen deutlich gesteigert. The separation efficiency is increased, the particles protect the separate and significantly increase the maximum possible loading of particles.

Ein Ausführungsbeispiel der Erfindung ist in Zeichnungen dargestellt. An embodiment of the invention is illustrated in drawings. Es zeigt It shows

Zeichnung 1 eine Aufsicht mit Einlaßöffnung ( 1 ), 1 is a plan drawing of the inlet opening (1),

Zeichnung 2 eine Seitenansicht mit Einlaßöffnung ( 1 ). Drawing 2 is a side view with the inlet opening (1).

Claims (1)

  1. Kammer zur Trennung eines Teilchengemisches nach dem Gegenstromzentrifugationsverfahren, gekennzeichnet durch eine großvolumige, zentrale, in der Längsachse ausgerichtete Einlaßöffnung ( 1 ) und einen sich konstant vergrößernden Querschnitt ( 2 ), dergestalt, daß ein gleichmäßiger Strömungs verlauf und eine gleichmäßige Verteilung der Teilchen erreicht wird. Chamber for separating a particle mixture according to the Gegenstromzentrifugationsverfahren, characterized by a large volume, a central, aligned in the longitudinal axis of the inlet opening (1) and a constantly increasing cross-section (2) such that a uniform flow running, and a uniform distribution of the particles is achieved.
DE19873700122 1987-01-03 1987-01-03 Chamber for separating a particle mixture according to the countercurrent centrifugation method Withdrawn DE3700122A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE19873700122 DE3700122A1 (en) 1987-01-03 1987-01-03 Chamber for separating a particle mixture according to the countercurrent centrifugation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19873700122 DE3700122A1 (en) 1987-01-03 1987-01-03 Chamber for separating a particle mixture according to the countercurrent centrifugation method

Publications (1)

Publication Number Publication Date
DE3700122A1 true true DE3700122A1 (en) 1988-07-14

Family

ID=6318444

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19873700122 Withdrawn DE3700122A1 (en) 1987-01-03 1987-01-03 Chamber for separating a particle mixture according to the countercurrent centrifugation method

Country Status (1)

Country Link
DE (1) DE3700122A1 (en)

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996033023A1 (en) * 1995-04-18 1996-10-24 Cobe Laboratories, Inc. Particle separation apparatus and method
NL1002569C2 (en) * 1996-03-11 1997-09-12 Univ Delft Tech A method for performing a treatment in the presence of a centrifugal force, and apparatus therefor.
US5674173A (en) * 1995-04-18 1997-10-07 Cobe Laboratories, Inc. Apparatus for separating particles
US5906570A (en) * 1995-04-18 1999-05-25 Cobe Laboratories, Inc. Particle filter apparatus
US6022306A (en) * 1995-04-18 2000-02-08 Cobe Laboratories, Inc. Method and apparatus for collecting hyperconcentrated platelets
US6051146A (en) * 1998-01-20 2000-04-18 Cobe Laboratories, Inc. Methods for separation of particles
US6053856A (en) * 1995-04-18 2000-04-25 Cobe Laboratories Tubing set apparatus and method for separation of fluid components
US6153113A (en) 1999-02-22 2000-11-28 Cobe Laboratories, Inc. Method for using ligands in particle separation
US6334842B1 (en) 1999-03-16 2002-01-01 Gambro, Inc. Centrifugal separation apparatus and method for separating fluid components
US6354986B1 (en) 2000-02-16 2002-03-12 Gambro, Inc. Reverse-flow chamber purging during centrifugal separation
WO2008156906A1 (en) * 2007-06-19 2008-12-24 Caridianbct, Inc. Blood processing apparatus with flared cell capture chamber
US7708889B2 (en) 2002-04-16 2010-05-04 Caridianbct, Inc. Blood component processing system method
US7963901B2 (en) 2008-09-12 2011-06-21 Caridianbct, Inc. Blood processing apparatus with cell capture chamber with protruding inlet
US9248446B2 (en) 2013-02-18 2016-02-02 Terumo Bct, Inc. System for blood separation with a separation chamber having an internal gravity valve

Cited By (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996033023A1 (en) * 1995-04-18 1996-10-24 Cobe Laboratories, Inc. Particle separation apparatus and method
US6071422A (en) * 1995-04-18 2000-06-06 Cobe Laboratories, Inc. Particle separation method and apparatus
US6053856A (en) * 1995-04-18 2000-04-25 Cobe Laboratories Tubing set apparatus and method for separation of fluid components
US5674173A (en) * 1995-04-18 1997-10-07 Cobe Laboratories, Inc. Apparatus for separating particles
US5722926A (en) * 1995-04-18 1998-03-03 Cobe Laboratories, Inc. Method for separating particles
US5906570A (en) * 1995-04-18 1999-05-25 Cobe Laboratories, Inc. Particle filter apparatus
US5913768A (en) * 1995-04-18 1999-06-22 Cobe Laboratories, Inc. Particle filter apparatus
US5951877A (en) * 1995-04-18 1999-09-14 Cobe Laboratories, Inc. Particle filter method
US6022306A (en) * 1995-04-18 2000-02-08 Cobe Laboratories, Inc. Method and apparatus for collecting hyperconcentrated platelets
US6180394B1 (en) 1996-03-11 2001-01-30 Bird Engineering B.V. Method of carrying out a treatment in the presence of a centrifugal force and an apparatus therefor
WO1997033687A1 (en) * 1996-03-11 1997-09-18 Bird Engineering B.V. Method of carrying out a treatment in the presence of a centrifugal force and an apparatus therefor
NL1002569C2 (en) * 1996-03-11 1997-09-12 Univ Delft Tech A method for performing a treatment in the presence of a centrifugal force, and apparatus therefor.
US6051146A (en) * 1998-01-20 2000-04-18 Cobe Laboratories, Inc. Methods for separation of particles
US6280622B1 (en) 1999-02-22 2001-08-28 Gambro, Inc. System for using ligands in particle separation
US6153113A (en) 1999-02-22 2000-11-28 Cobe Laboratories, Inc. Method for using ligands in particle separation
US6334842B1 (en) 1999-03-16 2002-01-01 Gambro, Inc. Centrifugal separation apparatus and method for separating fluid components
US6514189B1 (en) 1999-03-16 2003-02-04 Gambro, Inc. Centrifugal separation method for separating fluid components
US6354986B1 (en) 2000-02-16 2002-03-12 Gambro, Inc. Reverse-flow chamber purging during centrifugal separation
US7708889B2 (en) 2002-04-16 2010-05-04 Caridianbct, Inc. Blood component processing system method
WO2008156906A1 (en) * 2007-06-19 2008-12-24 Caridianbct, Inc. Blood processing apparatus with flared cell capture chamber
US7857744B2 (en) 2007-06-19 2010-12-28 Caridianbct, Inc. Blood processing apparatus with flared cell capture chamber and method
US7963901B2 (en) 2008-09-12 2011-06-21 Caridianbct, Inc. Blood processing apparatus with cell capture chamber with protruding inlet
US8226537B2 (en) 2008-09-12 2012-07-24 Terumo Bct, Inc. Blood processing apparatus with cell separation chamber with baffles
US9248446B2 (en) 2013-02-18 2016-02-02 Terumo Bct, Inc. System for blood separation with a separation chamber having an internal gravity valve

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