DE3419823A1 - Verfahren und einrichtung zur vorreinigung und anreicherung von partikeln aus zellen - Google Patents
Verfahren und einrichtung zur vorreinigung und anreicherung von partikeln aus zellenInfo
- Publication number
- DE3419823A1 DE3419823A1 DE19843419823 DE3419823A DE3419823A1 DE 3419823 A1 DE3419823 A1 DE 3419823A1 DE 19843419823 DE19843419823 DE 19843419823 DE 3419823 A DE3419823 A DE 3419823A DE 3419823 A1 DE3419823 A1 DE 3419823A1
- Authority
- DE
- Germany
- Prior art keywords
- particles
- chloroplasts
- cells
- concentration
- filling cylinder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 31
- 239000002245 particle Substances 0.000 title claims abstract description 25
- 238000000746 purification Methods 0.000 title abstract description 3
- 238000000926 separation method Methods 0.000 claims abstract description 4
- 239000011521 glass Substances 0.000 claims description 13
- 238000011049 filling Methods 0.000 claims description 12
- 238000004140 cleaning Methods 0.000 claims description 9
- 210000004027 cell Anatomy 0.000 abstract description 24
- 210000003763 chloroplast Anatomy 0.000 abstract description 21
- 238000002955 isolation Methods 0.000 abstract description 7
- 238000001914 filtration Methods 0.000 abstract description 3
- 239000011324 bead Substances 0.000 description 11
- 229930002875 chlorophyll Natural products 0.000 description 9
- 235000019804 chlorophyll Nutrition 0.000 description 9
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 9
- 239000000725 suspension Substances 0.000 description 7
- 241000195493 Cryptophyta Species 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 238000004062 sedimentation Methods 0.000 description 3
- 241000195619 Euglena gracilis Species 0.000 description 2
- 229910052793 cadmium Inorganic materials 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 210000003463 organelle Anatomy 0.000 description 2
- 230000000243 photosynthetic effect Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 239000010791 domestic waste Substances 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 231100000317 environmental toxin Toxicity 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000013213 extrapolation Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000001738 isopycnic centrifugation Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000010801 sewage sludge Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/005—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19843419823 DE3419823A1 (de) | 1984-05-26 | 1984-05-26 | Verfahren und einrichtung zur vorreinigung und anreicherung von partikeln aus zellen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19843419823 DE3419823A1 (de) | 1984-05-26 | 1984-05-26 | Verfahren und einrichtung zur vorreinigung und anreicherung von partikeln aus zellen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3419823A1 true DE3419823A1 (de) | 1985-11-28 |
| DE3419823C2 DE3419823C2 (enrdf_load_stackoverflow) | 1992-11-12 |
Family
ID=6237015
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19843419823 Granted DE3419823A1 (de) | 1984-05-26 | 1984-05-26 | Verfahren und einrichtung zur vorreinigung und anreicherung von partikeln aus zellen |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE3419823A1 (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104174475A (zh) * | 2013-05-28 | 2014-12-03 | 四川好医生药业集团有限公司 | 匀浆瓶 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3647070A (en) * | 1970-06-11 | 1972-03-07 | Technicon Corp | Method and apparatus for the provision of fluid interface barriers |
| US3972812A (en) * | 1975-05-08 | 1976-08-03 | Becton, Dickinson And Company | Blood serum separation filter disc |
| DE2912675A1 (de) * | 1978-04-03 | 1979-10-04 | Du Pont | Vorrichtung zum trennen von fluiden |
-
1984
- 1984-05-26 DE DE19843419823 patent/DE3419823A1/de active Granted
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3647070A (en) * | 1970-06-11 | 1972-03-07 | Technicon Corp | Method and apparatus for the provision of fluid interface barriers |
| US3972812A (en) * | 1975-05-08 | 1976-08-03 | Becton, Dickinson And Company | Blood serum separation filter disc |
| DE2912675A1 (de) * | 1978-04-03 | 1979-10-04 | Du Pont | Vorrichtung zum trennen von fluiden |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104174475A (zh) * | 2013-05-28 | 2014-12-03 | 四川好医生药业集团有限公司 | 匀浆瓶 |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3419823C2 (enrdf_load_stackoverflow) | 1992-11-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| 8127 | New person/name/address of the applicant |
Owner name: GSF - FORSCHUNGSZENTRUM FUER UMWELT UND GESUNDHEIT |
|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |