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DE2758036A1 - Adsorbent for separating bio-polymers - pref. comprising polymeric carrier with grafted dye gps. with nucleic acid affinity - Google Patents

Adsorbent for separating bio-polymers - pref. comprising polymeric carrier with grafted dye gps. with nucleic acid affinity

Info

Publication number
DE2758036A1
DE2758036A1 DE19772758036 DE2758036A DE2758036A1 DE 2758036 A1 DE2758036 A1 DE 2758036A1 DE 19772758036 DE19772758036 DE 19772758036 DE 2758036 A DE2758036 A DE 2758036A DE 2758036 A1 DE2758036 A1 DE 2758036A1
Authority
DE
Germany
Prior art keywords
pref
affinity
acryloylamino
adsorbent
specific
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19772758036
Other languages
German (de)
Inventor
Werner Prof Dr Rer Nat Mueller
Hans Dr Rer Nat Buenemann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Priority to DE19772758036 priority Critical patent/DE2758036A1/en
Publication of DE2758036A1 publication Critical patent/DE2758036A1/en
Application status is Withdrawn legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26Synthetic macromolecular compounds
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26Synthetic macromolecular compounds
    • B01J20/265Synthetic macromolecular compounds modified or post-treated polymers
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08FMACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F267/00Macromolecular compounds obtained by polymerising monomers on to polymers of unsaturated polycarboxylic acids or derivatives thereof as defined in group C08F22/00
    • C08F267/10Macromolecular compounds obtained by polymerising monomers on to polymers of unsaturated polycarboxylic acids or derivatives thereof as defined in group C08F22/00 on to polymers of amides or imides
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08FMACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F291/00Macromolecular compounds obtained by polymerising monomers on to macromolecular compounds according to more than one of the groups C08F251/00 - C08F289/00
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; MISCELLANEOUS COMPOSITIONS; MISCELLANEOUS APPLICATIONS OF MATERIALS
    • C09BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00Diaryl- or thriarylmethane dyes
    • C09B11/04Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/10Amino derivatives of triarylmethanes
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; MISCELLANEOUS COMPOSITIONS; MISCELLANEOUS APPLICATIONS OF MATERIALS
    • C09BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B15/00Acridine dyes
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; MISCELLANEOUS COMPOSITIONS; MISCELLANEOUS APPLICATIONS OF MATERIALS
    • C09BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B17/00Azine dyes
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; MISCELLANEOUS COMPOSITIONS; MISCELLANEOUS APPLICATIONS OF MATERIALS
    • C09BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B19/00Oxazine dyes
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; MISCELLANEOUS COMPOSITIONS; MISCELLANEOUS APPLICATIONS OF MATERIALS
    • C09BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B21/00Thiazine dyes
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; MISCELLANEOUS COMPOSITIONS; MISCELLANEOUS APPLICATIONS OF MATERIALS
    • C09BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B69/00Dyes not provided for by a single group of this subclass
    • C09B69/10Polymeric dyes; Reaction products of dyes with monomers or with macromolecular compounds

Abstract

(A) Adsorbent for affinity-specific sepn. of macromolecular materials, esp. biopolymers, comprises a polymeric carrier to which a gp. with affinity for the biopolymer is covalently bonded either directly or via a polymeric "spacer". Pref. the carrier is a microporous, low compressibility polymer to which >=2 comonomers are grafted as spacer, one of these monomers having affinity for the biopolymer. Pref. the polymer carries the residue of a dye of formula (I) or (II) as a base- and/or structure-specific complex former for nucleic acids. (where X is CH or N; Y is O, S, NH or R'-R4 are each H or Me; A circled negative is an anion, e.g. chloride, perchlorate or oxalate), or the residue of an intercalating dye contg. a polycyclic (e.g. 2-4 ring) planar condensed ring system opt. contg. heteroatoms, e.g. N, O or S. (B) Dyes of formulae (I) and (II) are new, provided that at least one R' gp. is acryloyl. Specific new dyes are acryloylamino-malachite green (III), acryloylamino-phenyl neutral red (IV), acridine derivs. (V) and acryloylamino-phenanthridine derivs. (VI). (R is H or acryloyl; R1 is H or Me).
DE19772758036 1977-12-24 1977-12-24 Adsorbent for separating bio-polymers - pref. comprising polymeric carrier with grafted dye gps. with nucleic acid affinity Withdrawn DE2758036A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE19772758036 DE2758036A1 (en) 1977-12-24 1977-12-24 Adsorbent for separating bio-polymers - pref. comprising polymeric carrier with grafted dye gps. with nucleic acid affinity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19772758036 DE2758036A1 (en) 1977-12-24 1977-12-24 Adsorbent for separating bio-polymers - pref. comprising polymeric carrier with grafted dye gps. with nucleic acid affinity

Publications (1)

Publication Number Publication Date
DE2758036A1 true DE2758036A1 (en) 1979-07-05

Family

ID=6027301

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19772758036 Withdrawn DE2758036A1 (en) 1977-12-24 1977-12-24 Adsorbent for separating bio-polymers - pref. comprising polymeric carrier with grafted dye gps. with nucleic acid affinity

Country Status (1)

Country Link
DE (1) DE2758036A1 (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0339869A2 (en) * 1988-04-26 1989-11-02 Minnesota Mining And Manufacturing Company Novel phenazine dyes
EP0342052A2 (en) * 1988-05-13 1989-11-15 S.A. Vilmax Novel monomers for producing coloured polymers; coloured polymers; process for preparing said polymers and for separating substances biologically active trough the use of said polymers
EP0366042A2 (en) * 1988-10-24 1990-05-02 Mitsubishi Kasei Corporation Adsorbent for optical resolution and resolution method employing it
DE4028355A1 (en) * 1989-09-06 1991-03-14 Sartorius Gmbh Microporous adsorbent
US5019350A (en) * 1986-02-13 1991-05-28 Pfizer Hospital Products, Inc. Fluorescent polymers
DE4122851A1 (en) * 1990-07-10 1992-01-16 Sartorius Gmbh Prodn. of porous, non-particulate permeable matrix
US5428161A (en) * 1984-10-01 1995-06-27 Minnesota Mining And Manufacturing Company Phenazine dyes
CN103189356A (en) * 2010-09-29 2013-07-03 英特维特国际股份有限公司 N-heteroaryl compounds
CN105001665A (en) * 2014-04-15 2015-10-28 苏州嘉恒生物科技有限公司 Preparation and applications of novel nucleic acid dye

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5428161A (en) * 1984-10-01 1995-06-27 Minnesota Mining And Manufacturing Company Phenazine dyes
US5019350A (en) * 1986-02-13 1991-05-28 Pfizer Hospital Products, Inc. Fluorescent polymers
EP0339869A3 (en) * 1988-04-26 1991-11-13 Minnesota Mining And Manufacturing Company Novel phenazine dyes
EP0339869A2 (en) * 1988-04-26 1989-11-02 Minnesota Mining And Manufacturing Company Novel phenazine dyes
EP0342052A3 (en) * 1988-05-13 1990-06-13 S.A. Vilmax Novel monomers for producing coloured polymers; coloured polymers; process for preparing said polymers and for separating substances biologically active trough the use of said polymers
EP0342052A2 (en) * 1988-05-13 1989-11-15 S.A. Vilmax Novel monomers for producing coloured polymers; coloured polymers; process for preparing said polymers and for separating substances biologically active trough the use of said polymers
EP0366042A3 (en) * 1988-10-24 1991-08-21 Mitsubishi Kasei Corporation Adsorbent for optical resolution and resolution method employing it
EP0366042A2 (en) * 1988-10-24 1990-05-02 Mitsubishi Kasei Corporation Adsorbent for optical resolution and resolution method employing it
US5096971A (en) * 1988-10-24 1992-03-17 Mitsubishi Kasei Corporation Adsorbent for optical resolution and resolution method employing it
DE4028355A1 (en) * 1989-09-06 1991-03-14 Sartorius Gmbh Microporous adsorbent
DE4122851A1 (en) * 1990-07-10 1992-01-16 Sartorius Gmbh Prodn. of porous, non-particulate permeable matrix
CN103189356A (en) * 2010-09-29 2013-07-03 英特维特国际股份有限公司 N-heteroaryl compounds
JP2014500235A (en) * 2010-09-29 2014-01-09 インターベツト・インターナシヨナル・ベー・ベー N- heteroaryl compounds
CN103189356B (en) * 2010-09-29 2015-11-25 英特维特国际股份有限公司 N- heteroaryl aryl compounds
KR101916481B1 (en) * 2010-09-29 2018-11-07 인터벳 인터내셔널 비.브이. N-heteroaryl compounds
CN105001665A (en) * 2014-04-15 2015-10-28 苏州嘉恒生物科技有限公司 Preparation and applications of novel nucleic acid dye
CN105001665B (en) * 2014-04-15 2017-05-31 苏州嘉恒生物科技有限公司 A novel nucleic acid dye of Preparation and Application

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